Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,449,738 +G coding (1106/1254 nt) ytfR → YtfR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,449,7371.G100.0% 751.9 / NA 233C369G (TGC→GGC) ytfRYtfR
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (118/115);  total (118/115)

Only 100 of 245 total aligned reads displayed.

GCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACA  >  NC_000913/4449645‑4449824
                                                                                             |                                                                                       
gCTTCTACCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgct                                                                                          >  1:3639223/1‑93 (MQ=255)
 cTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgct‑g                                                                                        <  1:4166829/93‑1 (MQ=255)
  ttCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgct‑gg                                                                                       >  2:5492371/1‑92 (MQ=255)
   tCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgct‑ggc                                                                                      <  2:2326677/93‑3 (MQ=255)
   tCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGCGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGgct‑ggc                                                                                      >  1:913320/1‑91 (MQ=255)
       cccGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACg                                                                                   <  1:4303922/93‑1 (MQ=255)
         cGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTc                                                                                 <  1:4592381/93‑1 (MQ=255)
         cGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTc                                                                                 <  2:2203088/93‑1 (MQ=255)
         cGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGAGCGTCTGGCTACggc                                                                                 >  1:2087985/1‑91 (MQ=255)
          ggAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTcc                                                                                >  2:3955735/1‑93 (MQ=255)
           gAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTccc                                                                               <  2:1307143/93‑1 (MQ=255)
            aaGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCa                                                                              >  2:3722977/1‑93 (MQ=255)
            aaGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCa                                                                              <  2:1538656/93‑1 (MQ=255)
              gACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCAtt                                                                            <  2:4518439/93‑1 (MQ=255)
               aCAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCAttt                                                                           <  1:5834950/93‑1 (MQ=255)
                cAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTc                                                                          <  1:4628585/93‑1 (MQ=255)
                cAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTc                                                                          >  1:806509/1‑93 (MQ=255)
                  ggAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTccc                                                                        <  1:1029859/93‑1 (MQ=255)
                  ggAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTccc                                                                        >  1:4897098/1‑93 (MQ=255)
                     aaaCCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCa                                                                     >  2:3071823/1‑93 (MQ=255)
                       aCCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCaaa                                                                   <  2:5079875/93‑1 (MQ=255)
                       aCCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCaaa                                                                   <  1:1626114/93‑1 (MQ=255)
                       aCCGATAGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCaaa                                                                   >  1:6152689/1‑93 (MQ=255)
                        ccGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAg                                                                  >  2:2026164/1‑93 (MQ=255)
                        ccGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAg                                                                  >  2:6266046/1‑93 (MQ=255)
                        ccGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAg                                                                  <  1:5566822/93‑1 (MQ=255)
                        ccGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAg                                                                  <  1:5424887/93‑1 (MQ=255)
                        ccGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAg                                                                  >  2:2095053/1‑93 (MQ=255)
                          gATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGaa                                                                <  1:3096110/93‑1 (MQ=255)
                            tGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACa                                                              >  1:1767593/1‑93 (MQ=255)
                            tGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACa                                                              <  2:3407419/93‑1 (MQ=255)
                              gCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGc                                                            <  2:6167803/93‑1 (MQ=255)
                               catcatCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCa                                                           <  2:416690/93‑1 (MQ=255)
                               catcatCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCa                                                           <  2:1932941/93‑1 (MQ=255)
                                 tcatcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGAGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAag                                                         >  2:1777441/1‑93 (MQ=255)
                                    tcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAgaga                                                      >  2:2492666/1‑93 (MQ=255)
                                    tcGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAgaga                                                      >  2:4639905/1‑93 (MQ=255)
                                     cGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAt                                                     >  1:4946712/1‑93 (MQ=255)
                                     cGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAt                                                     <  2:5709145/93‑1 (MQ=255)
                                     cGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAt                                                     <  2:5647095/93‑1 (MQ=255)
                                      gCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAtt                                                    <  2:1833358/93‑1 (MQ=255)
                                       cTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTg                                                   >  2:1667748/1‑93 (MQ=255)
                                         gccgccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGcc                                                 >  1:6216845/1‑93 (MQ=255)
                                          ccgccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCg                                                >  1:5924115/1‑93 (MQ=255)
                                            gccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGaa                                              >  2:1451482/1‑93 (MQ=255)
                                             ccTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAAc                                             <  1:4369277/93‑1 (MQ=255)
                                               tCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGGCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGc                                           >  1:5785159/1‑93 (MQ=255)
                                                 ggTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCtt                                         <  2:3845614/93‑1 (MQ=255)
                                                  gTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCttt                                        >  2:6002329/1‑93 (MQ=255)
                                                   tGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTa                                       <  1:999639/93‑1 (MQ=255)
                                                   tGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTa                                       <  1:4704274/93‑1 (MQ=255)
                                                     cGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATc                                     <  2:4097300/93‑1 (MQ=255)
                                                     cGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATc                                     <  1:2889073/93‑1 (MQ=255)
                                                     cGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTGCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATc                                     <  1:1703548/93‑1 (MQ=255)
                                                      gggAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATcc                                    >  2:261053/1‑93 (MQ=255)
                                                        gAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGc                                  >  1:2963255/1‑93 (MQ=255)
                                                        gAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGc                                  >  2:4594891/1‑93 (MQ=255)
                                                         aaaaTATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGcc                                 >  1:3122514/1‑93 (MQ=255)
                                                           aaTATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAg                               <  1:271560/93‑1 (MQ=255)
                                                            atatCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGc                              <  1:3823246/93‑1 (MQ=255)
                                                             tatCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCt                             <  1:4768625/93‑1 (MQ=255)
                                                              atcatcCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCtt                            <  2:5889018/93‑1 (MQ=255)
                                                               tcatcCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTg                           <  1:3796228/93‑1 (MQ=255)
                                                               tcatcCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTg                           >  2:703919/1‑93 (MQ=255)
                                                                catcCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTgg                          >  1:2188592/1‑93 (MQ=255)
                                                                catcCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTgg                          <  2:1298624/93‑1 (MQ=255)
                                                                 atcCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGc                         >  2:2874536/1‑93 (MQ=255)
                                                                 atcCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGc                         <  1:1736283/93‑1 (MQ=255)
                                                                  tcCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCa                        >  2:1321184/1‑93 (MQ=255)
                                                                   ccTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCAt                       <  1:5986252/93‑1 (MQ=255)
                                                                    cTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCAtt                      <  1:2054193/93‑1 (MQ=255)
                                                                     tCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTc                     >  1:3260397/1‑93 (MQ=255)
                                                                     tCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTc                     <  1:5197478/93‑1 (MQ=255)
                                                                       gCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGc                   <  2:5287091/93‑1 (MQ=255)
                                                                        ctctCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGca                  >  2:94327/1‑93 (MQ=255)
                                                                        ctctCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGca                  >  2:4420992/1‑93 (MQ=255)
                                                                        ctctCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGca                  <  1:6062823/93‑1 (MQ=255)
                                                                        ctctCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGca                  <  1:5406807/93‑1 (MQ=255)
                                                                         tctcCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGcac                 >  2:187159/1‑93 (MQ=255)
                                                                         tctcCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGcac                 >  1:707876/1‑93 (MQ=255)
                                                                         tctcCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCCTTATCCGCCAGCTTGGCATTCGcac                 >  2:3002204/1‑93 (MQ=255)
                                                                           tcCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGc                         >  2:3975453/1‑83 (MQ=255)
                                                                           tcCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGc                         <  1:3975453/83‑1 (MQ=255)
                                                                           tcCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCacac               >  1:520839/1‑93 (MQ=255)
                                                                            ccAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACAcc              >  2:252573/1‑93 (MQ=255)
                                                                            ccAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACAcc              >  1:1827738/1‑93 (MQ=255)
                                                                             cAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCt             >  1:1166502/1‑93 (MQ=255)
                                                                             cAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCt             >  1:1424839/1‑93 (MQ=255)
                                                                             cAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCt             >  2:4946171/1‑93 (MQ=255)
                                                                             cAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCt             >  2:5192372/1‑93 (MQ=255)
                                                                              aGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCtt            >  2:3307424/1‑93 (MQ=255)
                                                                               ggCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTc           <  1:2378161/93‑1 (MQ=255)
                                                                                 cccAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCaa         <  1:5595302/93‑1 (MQ=255)
                                                                                  ccAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAAc        <  2:4959665/93‑1 (MQ=255)
                                                                                   cAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACt       <  2:5397885/93‑1 (MQ=255)
                                                                                   cAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACt       <  2:727131/93‑1 (MQ=255)
                                                                                   cAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACt       >  2:853869/1‑93 (MQ=255)
                                                                                   cAGCGCGGCTGGCTACGTCCCAGTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACt       <  1:1196400/93‑1 (MQ=255)
                                                                                    aGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTg      >  2:467746/1‑93 (MQ=255)
                                                                                      cgcgGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGaa    <  2:2148667/93‑1 (MQ=255)
                                                                                             |                                                                                       
GCTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACA  >  NC_000913/4449645‑4449824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGAT  >  NC_000913/4449646‑4449830
                                                                                             |                                                                                            
CTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑GG                                                                                             <  SRR4302533.6133725/94‑1 (MQ=60)
CTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑GG                                                                                             <  SRR4302533.4192078/94‑1 (MQ=60)
CTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑GG                                                                                             <  SRR4302533.1309525/94‑1 (MQ=60)
 TTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGCGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑Ggc                                                                                            >  SRR4302533.918685/1‑92 (MQ=60)
    TGNCNGNAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTAC                                                                                          <  SRR4302533.2783379/94‑1 (MQ=60)
      CCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGT                                                                                        <  SRR4302533.4329816/94‑1 (MQ=60)
        CGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCC                                                                                      <  SRR4302533.4620199/94‑1 (MQ=60)
         GGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCC                                                                                     >  SRR4302533.1752186/1‑94 (MQ=60)
            AGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATT                                                                                  <  SRR4302533.4024885/94‑1 (MQ=60)
              ACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTC                                                                                >  SRR4302533.811247/1‑94 (MQ=60)
              ACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTC                                                                                <  SRR4302533.5870212/94‑1 (MQ=60)
              ACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTC                                                                                <  SRR4302533.5455744/94‑1 (MQ=60)
               CAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCC                                                                               <  SRR4302533.4656636/94‑1 (MQ=60)
                AGGAAAACCGATGGCATCATCGCTGCCGCCTTGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCC                                                                              >  SRR4302533.2610114/1‑94 (MQ=60)
                AGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCC                                                                              >  SRR4302533.4926770/1‑94 (MQ=60)
                 GGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCG                                                                             <  SRR4302533.1035912/94‑1 (MQ=60)
                   AAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCA                                                                           >  SRR4302533.5868495/1‑94 (MQ=60)
                   AAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCA                                                                           >  SRR4302533.5946094/1‑94 (MQ=60)
                   AAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCA                                                                           <  SRR4302533.2213854/94‑1 (MQ=60)
                    AAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCCA                                                                          >  SRR4302533.4499802/1‑94 (MQ=60)
                     AACCGATAGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAA                                                                         >  SRR4302533.6189716/1‑94 (MQ=60)
                      ACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAG                                                                        <  SRR4302533.1635963/94‑1 (MQ=60)
                       CCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGA                                                                       <  SRR4302533.5457558/94‑1 (MQ=60)
                       CCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGA                                                                       <  SRR4302533.5600471/94‑1 (MQ=60)
                        CGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAA                                                                      <  SRR4302533.5313928/94‑1 (MQ=60)
                         GATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAAC                                                                     >  SRR4302533.1700058/1‑94 (MQ=60)
                         GATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAAC                                                                     <  SRR4302533.5712410/94‑1 (MQ=60)
                         GATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAAC                                                                     <  SRR4302533.3115142/94‑1 (MQ=60)
                          ATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACA                                                                    >  SRR4302533.1778355/1‑94 (MQ=60)
                           TGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAG                                                                   >  SRR4302533.6292328/1‑94 (MQ=60)
                            GGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGC                                                                  <  SRR4302533.2859966/94‑1 (MQ=60)
                            GGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGC                                                                  <  SRR4302533.921761/94‑1 (MQ=60)
                             GCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCA                                                                 <  SRR4302533.3966586/94‑1 (MQ=60)
                             GCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCA                                                                 <  SRR4302533.5971004/94‑1 (MQ=60)
                              CATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAA                                                                >  SRR4302533.4711177/1‑94 (MQ=60)
                               ATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAG                                                               >  SRR4302533.4393864/1‑94 (MQ=60)
                                TCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGA                                                              <  SRR4302533.2441976/94‑1 (MQ=60)
                                 CATCGCTGCCGCCTCGGTGCGGGAAGATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAG                                                             <  SRR4302533.6122850/94‑1 (MQ=60)
                                 CATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGGGCGGCTGGCTACGTCCCATTTCTCGCAAAGACCAGCAAGAG                                                             >  SRR4302533.2571170/1‑94 (MQ=60)
                                 CATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAG                                                             <  SRR4302533.3797323/94‑1 (MQ=60)
                                  ATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGA                                                            >  SRR4302533.5527804/1‑94 (MQ=60)
                                   cccccccccgcgggggggggaaaattctctcccgCGCTCCCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAT                                                           <  SRR4302533.4822289/60‑1 (MQ=60)
                                   TCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGAT                                                           >  SRR4302533.4976652/1‑94 (MQ=60)
                                    CGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATT                                                          <  SRR4302533.3542270/94‑1 (MQ=60)
                                    CGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATT                                                          >  SRR4302533.5496502/1‑94 (MQ=60)
                                     GCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTG                                                         <  SRR4302533.2264667/94‑1 (MQ=60)
                                     GCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTG                                                         >  SRR4302533.3837385/1‑94 (MQ=60)
                                       TGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGCTTGCC                                                       >  SRR4302533.3125512/1‑94 (MQ=60)
                                       TGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCC                                                       >  SRR4302533.2107181/1‑94 (MQ=60)
                                       TGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCC                                                       >  SRR4302533.6254123/1‑94 (MQ=60)
                                       TGCCGCCTCGATGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCC                                                       <  SRR4302533.5603749/94‑1 (MQ=60)
                                        GCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCG                                                      >  SRR4302533.5959924/1‑94 (MQ=60)
                                          CGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAA                                                    >  SRR4302533.864256/1‑94 (MQ=60)
                                          CGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAA                                                    >  SRR4302533.5232204/1‑94 (MQ=60)
                                          CGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAA                                                    >  SRR4302533.5200593/1‑94 (MQ=60)
                                           GCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAAC                                                   >  SRR4302533.2999655/1‑94 (MQ=60)
                                            CCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACG                                                  >  SRR4302533.5637048/1‑94 (MQ=60)
                                            CCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACG                                                  <  SRR4302533.4745159/94‑1 (MQ=60)
                                            CCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACG                                                  <  SRR4302533.4395650/94‑1 (MQ=60)
                                             CTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGC                                                 <  SRR4302533.175958/94‑1 (MQ=60)
                                             CTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGC                                                 >  SRR4302533.1671090/1‑94 (MQ=60)
                                             CTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGGCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGC                                                 >  SRR4302533.5820098/1‑94 (MQ=60)
                                               CGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTT                                               >  SRR4302533.1182910/1‑94 (MQ=60)
                                                GGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTT                                              <  SRR4302533.1832715/94‑1 (MQ=60)
                                                 GTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTA                                             >  SRR4302533.6285117/1‑94 (MQ=60)
                                                  TGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTAT                                            <  SRR4302533.58860/94‑1 (MQ=60)
                                                  TGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTAT                                            <  SRR4302533.4732788/94‑1 (MQ=60)
                                                  TGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTAT                                            <  SRR4302533.4873915/94‑1 (MQ=60)
                                                  TGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTAT                                            <  SRR4302533.1005474/94‑1 (MQ=60)
                                                   GCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATC                                           <  SRR4302533.5273962/94‑1 (MQ=60)
                                                   GCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATC                                           >  SRR4302533.6039221/1‑94 (MQ=60)
                                                    CGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCC                                          <  SRR4302533.3232156/94‑1 (MQ=60)
                                                    CGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCC                                          <  SRR4302533.2906833/94‑1 (MQ=60)
                                                    CGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCC                                          <  SRR4302533.1734892/94‑1 (MQ=60)
                                                    CGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTGCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCC                                          <  SRR4302533.1713867/94‑1 (MQ=60)
                                                      GGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGC                                        >  SRR4302533.3691073/1‑94 (MQ=60)
                                                      GGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGC                                        >  SRR4302533.2981371/1‑94 (MQ=60)
                                                       GAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCC                                       >  SRR4302533.3185713/1‑94 (MQ=60)
                                                       GAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCC                                       >  SRR4302533.3141711/1‑94 (MQ=60)
                                                          AATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGC                                    <  SRR4302533.273213/94‑1 (MQ=60)
                                                           ATATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCT                                   <  SRR4302533.3846622/94‑1 (MQ=60)
                                                           AGATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCT                                   <  SRR4302533.3710834/94‑1 (MQ=60)
                                                            TATCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTT                                  <  SRR4302533.4797529/94‑1 (MQ=60)
                                                              TCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGG                                <  SRR4302533.3819465/94‑1 (MQ=60)
                                                              TCATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGG                                >  SRR4302533.2202076/1‑94 (MQ=60)
                                                               CATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGC                               >  SRR4302533.1037598/1‑94 (MQ=60)
                                                               CATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGC                               >  SRR4302533.4784778/1‑94 (MQ=60)
                                                               CATCCTCGCTCGCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGC                               <  SRR4302533.4996117/94‑1 (MQ=60)
                                                                ctgctgtaTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCA                              <  SRR4302533.3999655/86‑1 (MQ=60)
                                                                ATCCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCA                              <  SRR4302533.1746819/94‑1 (MQ=60)
                                                                  CCTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATT                            <  SRR4302533.6022503/94‑1 (MQ=60)
                                                                   CTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTC                           >  SRR4302533.3280509/1‑94 (MQ=60)
                                                                   CTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTC                           <  SRR4302533.2066866/94‑1 (MQ=60)
                                                                   CTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTC                           >  SRR4302533.4642003/1‑94 (MQ=60)
                                                                   CTCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTC                           >  SRR4302533.8880/1‑94 (MQ=60)
                                                                    TCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCG                          <  SRR4302533.2818009/94‑1 (MQ=60)
                                                                    TCGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCG                          <  SRR4302533.5228822/94‑1 (MQ=60)
                                                                     CGCTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGC                         <  SRR4302533.1382772/94‑1 (MQ=60)
                                                                       CTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCAC                       >  SRR4302533.712055/1‑94 (MQ=60)
                                                                       CTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCAC                       >  SRR4302533.3102519/1‑94 (MQ=60)
                                                                       CTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCAC                       >  SRR4302533.4236738/1‑94 (MQ=60)
                                                                       CTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCAC                       <  SRR4302533.6099525/94‑1 (MQ=60)
                                                                       CTCTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCAC                       <  SRR4302533.5439298/94‑1 (MQ=60)
                                                                         CTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACAC                     >  SRR4302533.758675/1‑94 (MQ=60)
                                                                         CTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACAC                     >  SRR4302533.523929/1‑94 (MQ=60)
                                                                         CTCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACAC                     >  SRR4302533.4351675/1‑94 (MQ=60)
                                                                          TCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACC                    >  SRR4302533.1838900/1‑94 (MQ=60)
                                                                          TCCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACC                    >  SRR4302533.4089297/1‑94 (MQ=60)
                                                                           CCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCT                   >  SRR4302533.1433457/1‑94 (MQ=60)
                                                                           CCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCT                   <  SRR4302533.6213825/94‑1 (MQ=60)
                                                                           CCAGGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCT                   >  SRR4302533.1173499/1‑94 (MQ=60)
                                                                              GGCCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCA                <  SRR4302533.2392876/94‑1 (MQ=60)
                                                                                CCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAAC              <  SRR4302533.5629110/94‑1 (MQ=60)
                                                                                CCCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAAC              <  SRR4302533.4974789/94‑1 (MQ=60)
                                                                                 CCAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACT             <  SRR4302533.2035511/94‑1 (MQ=60)
                                                                                  CAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTG            >  SRR4302533.2957019/1‑94 (MQ=60)
                                                                                  CAGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTA            >  SRR4302533.1951882/1‑94 (MQ=60)
                                                                                  CAGCGCGGCTGGCTACGTCCCAGTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTG            <  SRR4302533.1203588/94‑1 (MQ=60)
                                                                                   AGCGCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGA           >  SRR4302533.2093456/1‑94 (MQ=60)
                                                                                      GCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACA        >  SRR4302533.1600295/1‑94 (MQ=60)
                                                                                      GCGGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACA        >  SRR4302533.4635942/1‑94 (MQ=60)
                                                                                        GGCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAAC      >  SRR4302533.5825360/1‑94 (MQ=60)
                                                                                         GCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACC     <  SRR4302533.1786066/94‑1 (MQ=60)
                                                                                         GCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACC     <  SRR4302533.4855965/94‑1 (MQ=60)
                                                                                         GCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACC     <  SRR4302533.1134736/94‑1 (MQ=60)
                                                                                         GCTGGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCCTCAACTGAACAACC     >  SRR4302533.868879/1‑94 (MQ=60)
                                                                                          ctgGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCG    <  SRR4302533.5164779/91‑1 (MQ=60)
                                                                                          ctgGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCG    <  SRR4302533.1284801/91‑1 (MQ=60)
                                                                                          ctgGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTT‑GCATTCGGACCCCTTCCACTTAACAACCGA   >  SRR4302533.6123815/4‑94 (MQ=60)
                                                                                           tgGCTACGTCCCATTTCGCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGA   <  SRR4302533.3130979/92‑1 (MQ=60)
                                                                                           tgGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCATCTTGGCATTCGCACACCTTCAACTGAACAACCGA   <  SRR4302533.6179386/92‑1 (MQ=60)
                                                                                           tgGCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGA   >  SRR4302533.5933474/3‑94 (MQ=60)
                                                                                           tgGCTACGTCCCATTCCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGA   <  SRR4302533.1104929/92‑1 (MQ=60)
                                                                                           G‑GCTACGTCCCATTTCCCGGAAAGAACCGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGAT  >  SRR4302533.1870599/1‑94 (MQ=60)
                                                                                           G‑GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGAT  <  SRR4302533.1926616/94‑1 (MQ=60)
                                                                                             |                                                                                            
CTTCTGCCCGGAAGACAGGAAAACCGATGGCATCATCGCTGCCGCCTCGGTGCGGGAAAATATCATCCTCGCTCTCCAGGCCCAGCGCGGCT‑GCTACGTCCCATTTCCCGCAAAGAACAGCAAGAGATTGCCGAACGCTTTATCCGCCAGCTTGGCATTCGCACACCTTCAACTGAACAACCGAT  >  NC_000913/4449646‑4449830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑