Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,450,334	G→A	A64T (GCT→ACT)	ytfT →	putative transport system permease protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,450,334	0	G	A	98.9%	943.5 / ‑5.1	273	A64T (GCT→ACT)	ytfT	putative transport system permease protein
Reads supporting (aligned to +/- strand): ref base G (0/3); new base A (135/134); total (135/138)
Fisher's exact test for biased strand distribution p-value = 2.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 288 total aligned reads displayed.

GTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCC  >  NC_000913/4450244‑4450423

  cGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTcc                                                                                       <  2:9180320/93‑1 (MQ=255)

   gATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTccc                                                                                      >  2:873527/1‑93 (MQ=255)

    aTAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCg                                                                                     >  1:5499621/1‑93 (MQ=255)

    aTAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCCTGCAACTCCCg                                                                                     >  2:9644636/1‑93 (MQ=255)

     tAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGt                                                                                    >  1:8373343/1‑93 (MQ=255)

     tAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGt                                                                                    >  2:7154158/1‑93 (MQ=255)

      aGCCTTGTAGCCCCGCATTGCTGGCAGGTGGTGCGTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGtt                                                                                   <  2:8446616/93‑1 (MQ=255)

      aGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGtt                                                                                   <  2:1211259/93‑1 (MQ=255)

      aGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGtt                                                                                   >  1:625241/1‑93 (MQ=255)

      aGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGtt                                                                                   <  1:7319457/93‑1 (MQ=255)

       gCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTg                                                                                  >  1:461969/1‑93 (MQ=255)

         cTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTCACCGTGCAACTCCCGTTGCg                                                                                >  2:5782424/1‑93 (MQ=255)

         cTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCg                                                                                <  2:746158/93‑1 (MQ=255)

         cTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCg                                                                                >  2:333036/1‑93 (MQ=255)

             tAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTAc                                                                            <  1:9180118/93‑1 (MQ=255)

             tAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTAc                                                                            <  1:1150668/93‑1 (MQ=255)

               gCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTg                                                                          <  2:1126129/93‑1 (MQ=255)

                ccccGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTgg                                                                         <  2:4120625/93‑1 (MQ=255)

                 cccGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGc                                                                        <  1:9290637/93‑1 (MQ=255)

                 cccGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGAAATTCTTAACCGTGCAACTCACGTTGCGTTACTGGc                                                                        >  2:6125902/1‑93 (MQ=255)

                  ccGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCg                                                                       >  1:3757903/1‑93 (MQ=255)

                  ccGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCg                                                                       >  1:5271307/1‑93 (MQ=255)

                   cGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGTGATACTGGCGa                                                                      >  2:7000180/1‑93 (MQ=255)

                    gCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTACCCGTGCACCTCCCGTTGCGTTACTGGCGAt                                                                     <  2:2354023/93‑1 (MQ=255)

                    gCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGAt                                                                     >  2:262207/1‑93 (MQ=255)

                    gCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGAt                                                                     <  2:1235448/93‑1 (MQ=255)

                     cATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGAtt                                                                    >  2:633269/1‑93 (MQ=255)

                     cATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGAtt                                                                    <  1:10000467/93‑1 (MQ=255)

                     cATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGAtt                                                                    >  2:8125226/1‑93 (MQ=255)

                     cATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGAtt                                                                    >  1:3063161/1‑93 (MQ=255)

                     cATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGAtt                                                                    >  2:5416671/1‑93 (MQ=255)

                       tttCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTgg                                                                  >  2:206956/1‑93 (MQ=255)

                       tttCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTgg                                                                  >  2:606924/1‑93 (MQ=255)

                         tCTGGCAGGTGTTGCTTCAGTATGGGCGTTTGTTCGGTAGCCCCATAGGCATTCTTAACCGTGCACCTCCCGTTGCGTTACTGGCGATTGGCa                                                                <  2:8630612/93‑1 (MQ=255)

                         tCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCa                                                                <  2:6807914/93‑1 (MQ=255)

                         tCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCa                                                                <  1:3428939/93‑1 (MQ=255)

                         tCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCa                                                                <  1:7833133/93‑1 (MQ=255)

                         tCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCa                                                                <  1:7232669/93‑1 (MQ=255)

                           tGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATg                                                              >  1:5080740/1‑93 (MQ=255)

                            ggCAGGTGGTGCTGCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGa                                                             <  1:4242374/93‑1 (MQ=255)

                             gCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGAc                                                            >  1:3514131/1‑93 (MQ=255)

                             gCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGAc                                                            <  2:5336655/93‑1 (MQ=255)

                              cAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACg                                                           <  1:5403331/93‑1 (MQ=255)

                               aGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGc                                                          >  1:7758593/1‑93 (MQ=255)

                                ggtggtGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCt                                                         <  1:1407363/93‑1 (MQ=255)

                                ggtggtGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCt                                                         >  1:6539593/1‑93 (MQ=255)

                                 gtggtgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTg                                                        >  2:10033038/1‑93 (MQ=255)

                                 gtggtgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTg                                                        <  2:5486715/93‑1 (MQ=255)

                                   ggtgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGt                                                      <  2:2307970/93‑1 (MQ=255)

                                    gtgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTg                                                     >  1:2848083/1‑93 (MQ=255)

                                     tgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGAGGCTGGTGa                                                    >  1:8777822/1‑93 (MQ=255)

                                     tgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGa                                                    <  2:5943483/93‑1 (MQ=255)

                                     tgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGa                                                    <  1:8587313/93‑1 (MQ=255)

                                     tgCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGa                                                    <  1:1604950/93‑1 (MQ=255)

                                      gCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAt                                                   <  1:7909130/93‑1 (MQ=255)

                                      gCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAt                                                   <  1:9865469/93‑1 (MQ=255)

                                           aGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCa                                              >  1:9589457/1‑93 (MQ=255)

                                            ggATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCAc                                             <  1:4820176/93‑1 (MQ=255)

                                             gATGGGCGTTTGTTCGGTAGCCCCATAGAGATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCAcc                                            >  2:6223218/1‑93 (MQ=255)

                                              aTGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCg                                           <  1:9994093/93‑1 (MQ=255)

                                               tGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCgg                                          >  2:8908080/1‑93 (MQ=255)

                                                 ggCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTg                                        <  1:263839/93‑1 (MQ=255)

                                                 ggCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTg                                        >  1:7403253/1‑93 (MQ=255)

                                                 ggCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTg                                        <  1:9723176/93‑1 (MQ=255)

                                                 ggCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTg                                        >  2:5218581/1‑93 (MQ=255)

                                                  gCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTgg                                       >  2:3853920/1‑93 (MQ=255)

                                                   cGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTggg                                      <  1:6811986/93‑1 (MQ=255)

                                                     tttGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGAt                                    >  2:1471842/1‑93 (MQ=255)

                                                     tttGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGAt                                    >  2:349554/1‑93 (MQ=255)

                                                       tGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCAGGTGATCGCCACCGGTGGGATTg                                  >  2:7224852/1‑93 (MQ=255)

                                                         ttCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGAt                                >  2:9004208/1‑93 (MQ=255)

                                                           cGGTAGCCCCATAGACATTCTTAACCGTGCACCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGAtct                              <  2:4433574/93‑1 (MQ=255)

                                                            ggTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGAtctc                             <  2:4581425/93‑1 (MQ=255)

                                                             gTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGAtctct                            >  2:249673/1‑93 (MQ=255)

                                                             gTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGAtctct                            <  1:3901964/93‑1 (MQ=255)

                                                              tAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGAtctctc                           <  1:8054142/93‑1 (MQ=255)

                                                               aGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTcc                          >  1:5832949/1‑93 (MQ=255)

                                                               aGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTcc                          >  1:2737709/1‑93 (MQ=255)

                                                                gCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCg                         >  2:2834068/1‑93 (MQ=255)

                                                                  cccATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTa                       <  2:3137773/93‑1 (MQ=255)

                                                                   ccATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAg                      >  1:2404340/1‑93 (MQ=255)

                                                                    cATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAgg                     >  1:7655976/1‑93 (MQ=255)

                                                                    cATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAgg                     <  1:6031769/93‑1 (MQ=255)

                                                                    cATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAgg                     >  1:3305437/1‑93 (MQ=255)

                                                                     aTAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAggg                    <  2:3059596/93‑1 (MQ=255)

                                                                      tAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAgggg                   <  2:3717828/93‑1 (MQ=255)

                                                                      tAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCCGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAgggg                   >  1:8909957/1‑93 (MQ=255)

                                                                        gACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCg                 <  1:2193055/93‑1 (MQ=255)

                                                                           aTTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTg              >  1:2073887/1‑93 (MQ=255)

                                                                            ttCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGa             >  2:1177678/1‑93 (MQ=255)

                                                                            ttCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGa             >  2:6470146/1‑93 (MQ=255)

                                                                            ttCTTAACCATGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGa             >  2:2516827/1‑93 (MQ=255)

                                                                             tCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGAt            <  2:7827791/93‑1 (MQ=255)

                                                                              cTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATg           <  1:2954/93‑1 (MQ=255)

                                                                               ttAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATgg          >  2:7993757/1‑93 (MQ=255)

                                                                                   ccGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCGCCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTAt      <  2:4917020/93‑1 (MQ=255)

                                                                                    cGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATc     <  1:100559/93‑1 (MQ=255)

                                                                                     gTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGCTGGCTATCg    >  1:8681449/1‑93 (MQ=255)

                                                                                     gTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCg    >  1:5709057/1‑93 (MQ=255)

                                                                                     gTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCg    >  1:5990440/1‑93 (MQ=255)

GTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCC  >  NC_000913/4450244‑4450423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGA  >  NC_000913/4450241‑4450426

CTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAA                                                                                              <  SRR4302540.8493012/94‑1 (MQ=60)

 TGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACTTTCTTAACCGTGCAAC                                                                                             >  SRR4302540.9074652/1‑94 (MQ=60)

 TGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAAC                                                                                             <  SRR4302540.4292713/94‑1 (MQ=60)

  GGTCGATAGCCTGGTAGCCCCGGATTTCTGGCAGGTTGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACT                                                                                            <  SRR4302540.5426908/94‑1 (MQ=60)

  GGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACT                                                                                            >  SRR4302540.7494202/1‑94 (MQ=60)

  GGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACT                                                                                            <  SRR4302540.7776027/94‑1 (MQ=60)

  GGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACT                                                                                            <  SRR4302540.2654454/94‑1 (MQ=60)

  GGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACT                                                                                            <  SRR4302540.2788205/94‑1 (MQ=60)

  GGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACT                                                                                            <  SRR4302540.4216871/94‑1 (MQ=60)

     CGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCC                                                                                         <  SRR4302540.672247/94‑1 (MQ=60)

      GATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCG                                                                                        >  SRR4302540.5560177/1‑94 (MQ=60)

       ATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGT                                                                                       >  SRR4302540.8470517/1‑94 (MQ=60)

       ATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGT                                                                                       <  SRR4302540.1600158/94‑1 (MQ=60)

       ATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGT                                                                                       >  SRR4302540.8470518/1‑94 (MQ=60)

        TAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTT                                                                                      >  SRR4302540.631891/1‑94 (MQ=60)

         AGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTG                                                                                     <  SRR4302540.7401206/94‑1 (MQ=60)

         AGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTG                                                                                     >  SRR4302540.466897/1‑94 (MQ=60)

          GCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGC                                                                                    <  SRR4302540.9712629/94‑1 (MQ=60)

           CCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCG                                                                                   >  SRR4302540.7833567/1‑94 (MQ=60)

            CTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGTCCCATAGTCCTTCTTAACCGTGCAGCTCCCGTTGCGT                                                                                  >  SRR4302540.8585423/1‑94 (MQ=60)

            CTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGT                                                                                  >  SRR4302540.3259873/1‑94 (MQ=60)

            CTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGT                                                                                  >  SRR4302540.10117408/1‑94 (MQ=60)

             TGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTT                                                                                 <  SRR4302540.9245095/94‑1 (MQ=60)

             TGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTT                                                                                 >  SRR4302540.5388526/1‑94 (MQ=60)

             TGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCCTT                                                                                 >  SRR4302540.5210357/1‑94 (MQ=60)

               GTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTAC                                                                               <  SRR4302540.9032792/94‑1 (MQ=60)

               GTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTAC                                                                               <  SRR4302540.6738759/94‑1 (MQ=60)

                TAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACT                                                                              <  SRR4302540.3359427/94‑1 (MQ=60)

                TAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACT                                                                              >  SRR4302540.2414377/1‑94 (MQ=60)

                TAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACT                                                                              <  SRR4302540.8882338/94‑1 (MQ=60)

                TAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACT                                                                              <  SRR4302540.9290224/94‑1 (MQ=60)

                TAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACT                                                                              <  SRR4302540.1162662/94‑1 (MQ=60)

                TAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACT                                                                              <  SRR4302540.4327547/94‑1 (MQ=60)

                 AGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTG                                                                             >  SRR4302540.37339/1‑94 (MQ=60)

                 AGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTG                                                                             >  SRR4302540.882126/1‑94 (MQ=60)

                    CCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCG                                                                          >  SRR4302540.3798898/1‑94 (MQ=60)

                    CCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCG                                                                          <  SRR4302540.7874085/94‑1 (MQ=60)

                    CCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCG                                                                          <  SRR4302540.7874084/94‑1 (MQ=60)

                    CCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCG                                                                          >  SRR4302540.5328847/1‑94 (MQ=60)

                    CCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCG                                                                          >  SRR4302540.6233194/1‑94 (MQ=60)

                    CCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCG                                                                          <  SRR4302540.9401667/94‑1 (MQ=60)

                    CCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCG                                                                          >  SRR4302540.7965367/1‑94 (MQ=60)

                     CCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGA                                                                         <  SRR4302540.3330015/94‑1 (MQ=60)

                       GCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATT                                                                       <  SRR4302540.10067237/94‑1 (MQ=60)

                       GCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATT                                                                       >  SRR4302540.3096765/1‑94 (MQ=60)

                        GATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTG                                                                      <  SRR4302540.2803902/94‑1 (MQ=60)

                        CATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTG                                                                      <  SRR4302540.7938372/94‑1 (MQ=60)

                        CATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTG                                                                      <  SRR4302540.10116972/94‑1 (MQ=60)

                        CATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTG                                                                      <  SRR4302540.64421/94‑1 (MQ=60)

                        CATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTG                                                                      <  SRR4302540.8089277/94‑1 (MQ=60)

                         ATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGG                                                                     <  SRR4302540.5917318/94‑1 (MQ=60)

                         ATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGG                                                                     >  SRR4302540.2271479/1‑94 (MQ=60)

                            TCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCAT                                                                  <  SRR4302540.7924659/94‑1 (MQ=60)

                            TCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCAT                                                                  <  SRR4302540.3466198/94‑1 (MQ=60)

                            TCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCAT                                                                  <  SRR4302540.7313334/94‑1 (MQ=60)

                            TCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCAT                                                                  <  SRR4302540.2918364/94‑1 (MQ=60)

                            TCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCAT                                                                  <  SRR4302540.4013645/94‑1 (MQ=60)

                             CTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATG                                                                 <  SRR4302540.746531/94‑1 (MQ=60)

                             CTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATG                                                                 >  SRR4302540.3877534/1‑94 (MQ=60)

                             CTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATG                                                                 >  SRR4302540.5135670/1‑94 (MQ=60)

                             CTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATG                                                                 >  SRR4302540.8767384/1‑94 (MQ=60)

                               GGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATCGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGGGATTGGCATGAC                                                               >  SRR4302540.3552414/1‑94 (MQ=60)

                               GGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGAC                                                               >  SRR4302540.3552415/1‑94 (MQ=60)

                               GGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGAC                                                               <  SRR4302540.932969/94‑1 (MQ=60)

                               GGCAGGTGGTGCTGCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGAC                                                               <  SRR4302540.4288527/94‑1 (MQ=60)

                                GCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACG                                                              <  SRR4302540.5088351/94‑1 (MQ=60)

                                 CAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGC                                                             >  SRR4302540.7849250/1‑94 (MQ=60)

                                 CAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGC                                                             <  SRR4302540.5462736/94‑1 (MQ=60)

                                 CAGGTGGTGCTTCAGGATGGGCGTTTCTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGC                                                             <  SRR4302540.5462737/94‑1 (MQ=60)

                                  AGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCT                                                            >  SRR4302540.6612162/1‑94 (MQ=60)

                                   GGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTG                                                           <  SRR4302540.4840766/94‑1 (MQ=60)

                                   GGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTG                                                           <  SRR4302540.3761467/94‑1 (MQ=60)

                                   GGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTG                                                           <  SRR4302540.1422516/94‑1 (MQ=60)

                                      GGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTG                                                        >  SRR4302540.5962020/1‑94 (MQ=60)

                                      GGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTG                                                        >  SRR4302540.2878922/1‑94 (MQ=60)

                                       GTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGAGGCTGGTGA                                                       >  SRR4302540.8879476/1‑94 (MQ=60)

                                       GTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGA                                                       >  SRR4302540.8879477/1‑94 (MQ=60)

                                        TGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAT                                                      <  SRR4302540.1622514/94‑1 (MQ=60)

                                        TGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAT                                                      <  SRR4302540.5616365/94‑1 (MQ=60)

                                        TGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAT                                                      <  SRR4302540.8687139/94‑1 (MQ=60)

                                         GCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATC                                                     <  SRR4302540.8001351/94‑1 (MQ=60)

                                         GCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATC                                                     <  SRR4302540.9980713/94‑1 (MQ=60)

                                          CTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCG                                                    >  SRR4302540.3076811/1‑94 (MQ=60)

                                            TCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCC                                                  <  SRR4302540.456964/94‑1 (MQ=60)

                                             CAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCA                                                 >  SRR4302540.9702644/1‑94 (MQ=60)

                                             CAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGCGCCA                                                 >  SRR4302540.7766453/1‑94 (MQ=60)

                                              AGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCAC                                                <  SRR4302540.6566824/94‑1 (MQ=60)

                                               GGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACC                                               <  SRR4302540.4871773/94‑1 (MQ=60)

                                                 ATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGG                                             <  SRR4302540.10110561/94‑1 (MQ=60)

                                                  TGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGT                                            <  SRR4302540.8340584/94‑1 (MQ=60)

                                                   GGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTG                                           <  SRR4302540.3483075/94‑1 (MQ=60)

                                                   GGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTG                                           >  SRR4302540.7485729/1‑94 (MQ=60)

                                                   GGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATCGGCATGACGCTGGTGATCGCCACCGGTG                                           >  SRR4302540.1083795/1‑94 (MQ=60)

                                                    GGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGG                                          <  SRR4302540.266416/94‑1 (MQ=60)

                                                    GGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGG                                          <  SRR4302540.9837390/94‑1 (MQ=60)

                                                      CGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGA                                        <  SRR4302540.6887363/94‑1 (MQ=60)

                                                      CGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGTCGCTGGTGATCGCCACCGGTGGGA                                        >  SRR4302540.4794227/1‑94 (MQ=60)

                                                      CGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGT                                        >  SRR4302540.8058685/1‑94 (MQ=60)

                                                       GTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGAT                                       >  SRR4302540.26481/1‑94 (MQ=60)

                                                         TTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGCTGCTCGCCACCGGTGGGATTG                                     >  SRR4302540.3020764/1‑94 (MQ=60)

                                                           GTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGAT                                   >  SRR4302540.1507007/1‑94 (MQ=60)

                                                           GTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGAT                                   >  SRR4302540.7243642/1‑94 (MQ=60)

                                                           GTTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGAT                                   <  SRR4302540.7369424/94‑1 (MQ=60)

                                                            TTCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATC                                  <  SRR4302540.6115530/94‑1 (MQ=60)

                                                             TCGGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCT                                 <  SRR4302540.2751822/94‑1 (MQ=60)

                                                               GGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCT                               >  SRR4302540.7390001/1‑94 (MQ=60)

                                                               GGTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCT                               <  SRR4302540.3465531/94‑1 (MQ=60)

                                                               GATAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCT                               <  SRR4302540.9597565/94‑1 (MQ=60)

                                                                GTAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTC                              <  SRR4302540.3944293/94‑1 (MQ=60)

                                                                 TAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCC                             <  SRR4302540.8147673/94‑1 (MQ=60)

                                                                 TAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCC                             <  SRR4302540.832296/94‑1 (MQ=60)

                                                                 TAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCC                             >  SRR4302540.2767260/1‑94 (MQ=60)

                                                                 TAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCC                             <  SRR4302540.2598007/94‑1 (MQ=60)

                                                                 TAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCC                             <  SRR4302540.5392495/94‑1 (MQ=60)

                                                                 TAGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCC                             >  SRR4302540.5897448/1‑94 (MQ=60)

                                                                  AGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCG                            >  SRR4302540.5086567/1‑94 (MQ=60)

                                                                  AGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCG                            >  SRR4302540.1285410/1‑94 (MQ=60)

                                                                  AGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCG                            >  SRR4302540.6089960/1‑94 (MQ=60)

                                                                  AGCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCA                            >  SRR4302540.6089959/1‑94 (MQ=60)

                                                                   GCCCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGT                           >  SRR4302540.9805031/1‑94 (MQ=60)

                                                                   GCACCATAGACCTTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGT                           <  SRR4302540.2837143/94‑1 (MQ=60)

                                                                     CCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAG                         <  SRR4302540.1762038/94‑1 (MQ=60)

                                                                     CCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAG                         >  SRR4302540.2430270/1‑94 (MQ=60)

                                                                     CCCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAG                         >  SRR4302540.5588923/1‑94 (MQ=60)

                                                                      CCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCGACCGGTGGGATCGATCTCTCCGTTGG                        >  SRR4302540.9953412/1‑94 (MQ=60)

                                                                      CCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGG                        >  SRR4302540.7742175/1‑94 (MQ=60)

                                                                      CCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGG                        >  SRR4302540.3341628/1‑94 (MQ=60)

                                                                      CCATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGG                        >  SRR4302540.7595542/1‑94 (MQ=60)

                                                                       CATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGG                       <  SRR4302540.6098712/94‑1 (MQ=60)

                                                                        agagcCATTCTTCACCGTGCACCTCCCGTTGCGTTACTGGCGATTGGCATGACGCGGGTGATCGCCGCCGGTGGGATTGATCTCTCCGTAGGGG                      <  SRR4302540.2068046/89‑1 (MQ=60)

                                                                        ATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGG                      <  SRR4302540.8847297/94‑1 (MQ=60)

                                                                        ATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGG                      <  SRR4302540.2068047/94‑1 (MQ=60)

                                                                        ATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGG                      >  SRR4302540.9479238/1‑94 (MQ=60)

                                                                        ATAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCCGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGG                      >  SRR4302540.9013130/1‑94 (MQ=60)

                                                                         TAGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGC                     <  SRR4302540.13063/94‑1 (MQ=60)

                                                                          AGACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGACACCCGTGGGATTGATCTTTCCGTAGGGGCG                    >  SRR4302540.4106710/1‑94 (MQ=60)

                                                                           GACATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGG                   <  SRR4302540.2216741/94‑1 (MQ=60)

                                                                             CATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTG                 <  SRR4302540.2960886/94‑1 (MQ=60)

                                                                             CATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTG                 >  SRR4302540.9930787/1‑94 (MQ=60)

                                                                             CATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTG                 >  SRR4302540.2096188/1‑94 (MQ=60)

                                                                             CATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTG                 >  SRR4302540.9361020/1‑94 (MQ=60)

                                                                              ATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGA                >  SRR4302540.2161020/1‑94 (MQ=60)

                                                                              ATTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGA                >  SRR4302540.1054093/1‑94 (MQ=60)

                                                                               TTCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGAT               <  SRR4302540.9985227/94‑1 (MQ=60)

                                                                                TCTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATG              <  SRR4302540.2215176/94‑1 (MQ=60)

                                                                                 CTTAACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGG             <  SRR4302540.2985/94‑1 (MQ=60)

                                                                                     ACCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTAT         <  SRR4302540.5153222/94‑1 (MQ=60)

                                                                                      CCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATC        <  SRR4302540.5495183/94‑1 (MQ=60)

                                                                                      CCGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGGGATGGCTATC        >  SRR4302540.7287340/1‑94 (MQ=60)

                                                                                       CGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGCTGGCTATCG       >  SRR4302540.8782269/1‑94 (MQ=60)

                                                                                       CGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCG       >  SRR4302540.5771907/1‑94 (MQ=60)

                                                                                       CGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCG       >  SRR4302540.6056805/1‑94 (MQ=60)

                                                                                       CGTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCG       <  SRR4302540.101628/94‑1 (MQ=60)

                                                                                        GTGCAACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGC      >  SRR4302540.5896817/1‑94 (MQ=60)

                                                                                            AACTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGA  >  SRR4302540.9070323/1‑94 (MQ=60)

CTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATCGCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGA  >  NC_000913/4450241‑4450426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`