Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 93,625 C→T P154S (CCG→TCG)  murE → meso‑diaminopimelate‑adding enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091393,6250CT98.9% 1442.7 / ‑1.1 379P154S (CCG→TCG) murEmeso‑diaminopimelate‑adding enzyme
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base T (201/173);  total (204/175)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01

Only 100 of 387 total aligned reads displayed.

TACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGC‑GACGTTTTGCGCAATGGAAG  >  NC_000913/93534‑93715
                                                                                           |                                                                                           
 aCCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGa                                                                                          >  1:7889032/1‑94 (MQ=255)
  cccAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGAc                                                                                         >  1:10520731/1‑94 (MQ=255)
  cccAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGAc                                                                                         <  2:1041857/94‑1 (MQ=255)
  cccAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGAc                                                                                         <  2:1189395/94‑1 (MQ=255)
  cccAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGAc                                                                                         >  1:372026/1‑94 (MQ=255)
  cccAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGAc                                                                                         >  1:281780/1‑94 (MQ=255)
    cAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAg                                                                                       <  1:10164858/94‑1 (MQ=255)
    cAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAg                                                                                       >  2:7155393/1‑94 (MQ=255)
     aGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGa                                                                                      <  1:629986/94‑1 (MQ=255)
      gCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGaa                                                                                     >  1:2310833/1‑94 (MQ=255)
      gCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGaa                                                                                     >  1:5575861/1‑94 (MQ=255)
      gCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGaa                                                                                     <  2:1942139/94‑1 (MQ=255)
       cTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGaaa                                                                                    >  2:5600566/1‑94 (MQ=255)
         gTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAAt                                                                                  >  1:2640747/1‑94 (MQ=255)
         gTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAAt                                                                                  <  1:7242189/94‑1 (MQ=255)
          ttGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATa                                                                                 <  1:4038956/94‑1 (MQ=255)
            ggCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACa                                                                               >  2:3811073/1‑94 (MQ=255)
              cgcAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAAc                                                                             <  2:28058/94‑1 (MQ=255)
               gcAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGCAAGTGATCTCGACAGAAAATACAAcc                                                                            >  2:8675604/1‑94 (MQ=255)
                cAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCg                                                                           <  2:324073/94‑1 (MQ=255)
                cAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCg                                                                           <  1:7656422/94‑1 (MQ=255)
                cAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCg                                                                           >  2:5926820/1‑94 (MQ=255)
                cAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCg                                                                           <  2:4099964/94‑1 (MQ=255)
                 aGTGGAGCTAACTGCTTGGCGAAAGCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCgg                                                                          <  1:10360163/94‑1 (MQ=255)
                 aGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGTACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCgg                                                                          <  2:9450299/94‑1 (MQ=255)
                 aGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCgg                                                                          >  2:5391489/1‑94 (MQ=255)
                     gAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCg                                                                      <  2:4889537/94‑1 (MQ=255)
                      aGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCgg                                                                     >  1:3828485/1‑94 (MQ=255)
                         cAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAg                                                                  >  2:4292590/1‑94 (MQ=255)
                          aaCTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGt                                                                 >  1:8690768/1‑94 (MQ=255)
                           aCTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTc                                                                <  1:6862657/94‑1 (MQ=255)
                             tgtttGGCGAATTCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGa                                                              >  1:9351169/4‑94 (MQ=255)
                             tGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGa                                                              >  1:9015952/1‑94 (MQ=255)
                              gCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGAt                                                             >  1:9990986/1‑94 (MQ=255)
                                 tGGCGAAATCAGCGCGGTAATGGGCATCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGtt                                                          <  2:9270791/94‑1 (MQ=255)
                                 tGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGtt                                                          <  1:1702798/94‑1 (MQ=255)
                                  ggCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTc                                                         >  2:7777481/1‑94 (MQ=255)
                                    cGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAg                                                       <  2:4746151/94‑1 (MQ=255)
                                      aaaTCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCa                                                     <  1:474409/94‑1 (MQ=255)
                                       aaTCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCAt                                                    >  2:3404685/1‑94 (MQ=255)
                                        aTCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATg                                                   <  1:6766482/94‑1 (MQ=255)
                                        aTCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATg                                                   <  2:6114057/94‑1 (MQ=255)
                                         tCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGa                                                  <  1:3651366/94‑1 (MQ=255)
                                          cAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCCGTCGATGTTCAGCATGAg                                                 >  2:10543289/1‑94 (MQ=255)
                                          cAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAg                                                 <  2:1019806/94‑1 (MQ=255)
                                            gcgcGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCt                                               <  1:10011173/94‑1 (MQ=255)
                                             cgcgGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTg                                              <  2:1222547/94‑1 (MQ=255)
                                               cgGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGc                                            <  1:4880309/94‑1 (MQ=255)
                                               cgGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGc                                            <  1:8356147/94‑1 (MQ=255)
                                               cgGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGc                                            <  2:9824526/94‑1 (MQ=255)
                                                ggTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCg                                           <  1:6511275/94‑1 (MQ=255)
                                                   aaTGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCgggg                                        <  2:9655479/94‑1 (MQ=255)
                                                      gggCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCtg                                     <  1:215241/94‑1 (MQ=255)
                                                      gggCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCtg                                     >  1:7811915/1‑94 (MQ=255)
                                                        gCACCGTTTGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCtggt                                   <  2:2165745/94‑1 (MQ=255)
                                                        gCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCtggt                                   <  2:6700127/94‑1 (MQ=255)
                                                        gCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCtggg                                   >  1:5432590/1‑93 (MQ=255)
                                                           ccGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGa                                <  2:80806/94‑1 (MQ=255)
                                                            cGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGAt                               >  1:4108695/1‑94 (MQ=255)
                                                            cGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGAt                               >  2:7366100/1‑94 (MQ=255)
                                                            cGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGGGGAt                               >  1:3505842/1‑94 (MQ=255)
                                                             gTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTTAGCATGAGCTGGCGGGGCTGGTGGATc                              >  1:7051247/1‑94 (MQ=255)
                                                              ttGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGGTcg                             >  1:2573569/1‑93 (MQ=255)
                                                              ttGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGGTCa                             >  2:900897/1‑94 (MQ=255)
                                                              ttGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCa                             <  2:6269313/94‑1 (MQ=255)
                                                               tGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAg                            <  1:6671428/94‑1 (MQ=255)
                                                               tGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAg                            <  2:316039/94‑1 (MQ=255)
                                                                ggTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAgg                           >  1:977666/1‑94 (MQ=255)
                                                                ggTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAgg                           <  1:734917/94‑1 (MQ=255)
                                                                 gTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAggg                          <  1:7018058/94‑1 (MQ=255)
                                                                  tAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGgc                         <  2:8596900/94‑1 (MQ=255)
                                                                  gaaCGGCCTGTTGGGGAAAGTGACCTCGACAGTAATTACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGgc                         <  1:4523124/93‑1 (MQ=255)
                                                                   aaCGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGgcg                        <  1:8155392/94‑1 (MQ=255)
                                                                   aaCGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGgcg                        <  1:8567397/94‑1 (MQ=255)
                                                                    aCGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGgcgc                       <  2:2442584/94‑1 (MQ=255)
                                                                    aCGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGgcgc                       >  1:5137712/1‑94 (MQ=255)
                                                                     cGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGcgc‑g                     >  1:5931845/1‑94 (MQ=255)
                                                                        ccTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGC‑GACg                  >  2:6832950/1‑94 (MQ=255)
                                                                         ctgctgGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGC‑GACGt                 >  1:3332577/1‑94 (MQ=255)
                                                                         ctgctgGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGGGGATCAGGGCGC‑GACGt                 >  1:3942843/1‑94 (MQ=255)
                                                                           gctgGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGC‑GACGttt               <  1:4432673/94‑1 (MQ=255)
                                                                            ctgGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGC‑GACGtttt              >  1:1464581/1‑94 (MQ=255)
                                                                            ctgGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGC‑GACGtttt              >  2:1609594/1‑94 (MQ=255)
                                                                            ctgGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGC‑GACGgttt              >  1:206526/1‑94 (MQ=255)
                                                                             tgGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGC‑GACGTTTTg             <  1:1328347/94‑1 (MQ=255)
                                                                               gggAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGGTCAGGGGGCGGAGGTTTTgg            >  1:4323883/1‑93 (MQ=255)
                                                                               gggAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGC‑GACGgttttgg           >  1:9618643/1‑91 (MQ=255)
                                                                                 gAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGC‑GACGTTTTGCGCa         >  2:3665193/1‑94 (MQ=255)
                                                                                 gAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGC‑GACGTTTTGCGCa         <  1:297190/94‑1 (MQ=255)
                                                                                  aaaGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGC‑GACGTTTTGCGCaa        >  2:6587522/1‑94 (MQ=255)
                                                                                  aaaGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGATGGCGGGGCTGGTGGATCAGGGCGG‑GACGTTTTGCGCaa        >  1:7438966/1‑94 (MQ=255)
                                                                                   aaGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGC‑GACGTTTTGCGCAAt       >  2:2043189/1‑94 (MQ=255)
                                                                                   aaGTGAACTGGACAGAAAATACAACCGTTTCCGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGC‑GACGTTTTGCGCAAt       <  1:5058697/94‑1 (MQ=255)
                                                                                    aGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGC‑GACGTTTTGCGCAATg      <  1:9271916/94‑1 (MQ=255)
                                                                                     gTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATTAGGGCGC‑GACGTTTTGCGCAATgg     >  2:5747465/1‑94 (MQ=255)
                                                                                     atGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGC‑GACGTTTTGCGCAATgg     <  1:3219934/93‑1 (MQ=255)
                                                                                      tGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGC‑GACGTTTTGCGCAATGGa    >  2:8894061/1‑94 (MQ=255)
                                                                                       gATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGTGC‑GACGTTTTGCGCAATGGaa   >  1:9491907/1‑94 (MQ=255)
                                                                                       gATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGC‑GACGTTTTGCGCAATGGaa   >  2:8504108/1‑94 (MQ=255)
                                                                                        aTCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGC‑GACGTTTTGCGCAATGGAAg  >  1:9831294/1‑94 (MQ=255)
                                                                                           |                                                                                           
TACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGC‑GACGTTTTGCGCAATGGAAG  >  NC_000913/93534‑93715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 233 total aligned reads displayed.
CTACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTT  >  NC_000913/93533‑93718
                                                                                            |                                                                                             
CTACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTC                                                                                              >  SRR4302545.1958328/1‑94 (MQ=60)
 TACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCG                                                                                             <  SRR4302545.7025426/94‑1 (MQ=60)
 TACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCG                                                                                             >  SRR4302545.3504014/1‑94 (MQ=60)
  ACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGA                                                                                            >  SRR4302545.8112903/1‑94 (MQ=60)
  ACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGA                                                                                            <  SRR4302545.4854407/94‑1 (MQ=60)
   CCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGAC                                                                                           >  SRR4302545.287653/1‑94 (MQ=60)
   CCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGAC                                                                                           <  SRR4302545.38808/94‑1 (MQ=60)
   CCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGAC                                                                                           >  SRR4302545.380202/1‑94 (MQ=60)
     CAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAG                                                                                         >  SRR4302545.9547604/1‑94 (MQ=60)
     CAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAG                                                                                         <  SRR4302545.7228661/94‑1 (MQ=60)
     CAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAG                                                                                         <  SRR4302545.10465392/94‑1 (MQ=60)
      AGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGA                                                                                        <  SRR4302545.644103/94‑1 (MQ=60)
      AGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGA                                                                                        <  SRR4302545.157218/94‑1 (MQ=60)
       GCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAA                                                                                       >  SRR4302545.2367130/1‑94 (MQ=60)
       GCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAA                                                                                       >  SRR4302545.5723489/1‑94 (MQ=60)
       GCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAA                                                                                       >  SRR4302545.408317/1‑94 (MQ=60)
       GCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAA                                                                                       >  SRR4302545.766216/1‑94 (MQ=60)
        CTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAA                                                                                      <  SRR4302545.8721138/94‑1 (MQ=60)
         TGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAA                                                                                     >  SRR4302545.4146459/1‑94 (MQ=60)
         TGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAA                                                                                     >  SRR4302545.4146460/1‑94 (MQ=60)
         TGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAA                                                                                     >  SRR4302545.7940754/1‑94 (MQ=60)
         TGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAA                                                                                     >  SRR4302545.10085538/1‑94 (MQ=60)
         TGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTCGGGAAAGTGATCTCGACAGAAAA                                                                                     >  SRR4302545.5968661/1‑94 (MQ=60)
          GTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAAT                                                                                    >  SRR4302545.2640577/1‑94 (MQ=60)
          GTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAAT                                                                                    >  SRR4302545.2704298/1‑94 (MQ=60)
          GTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAAT                                                                                    <  SRR4302545.7444849/94‑1 (MQ=60)
           TTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATA                                                                                   <  SRR4302545.8744295/94‑1 (MQ=60)
           TTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATA                                                                                   <  SRR4302545.4140090/94‑1 (MQ=60)
            TGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATAC                                                                                  <  SRR4302545.10461561/94‑1 (MQ=60)
                GCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACC                                                                              <  SRR4302545.10903315/94‑1 (MQ=60)
                GCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACC                                                                              <  SRR4302545.4747809/94‑1 (MQ=60)
                 CAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAAGCG                                                                             >  SRR4302545.10374386/1‑94 (MQ=60)
                 CAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCG                                                                             >  SRR4302545.4614548/1‑94 (MQ=60)
                 CAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCG                                                                             <  SRR4302545.7873083/94‑1 (MQ=60)
                  AGTGGAGCTAACTGCTTGGCGAAAGCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGG                                                                            <  SRR4302545.10665576/94‑1 (MQ=60)
                  AGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGG                                                                            >  SRR4302545.4950815/1‑94 (MQ=60)
                  AGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGG                                                                            >  SRR4302545.2977407/1‑94 (MQ=60)
                    TGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTT                                                                          >  SRR4302545.7309129/1‑94 (MQ=60)
                    TGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTT                                                                          >  SRR4302545.6832300/1‑94 (MQ=60)
                    TGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTT                                                                          <  SRR4302545.5680363/94‑1 (MQ=60)
                     GGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTC                                                                         <  SRR4302545.496953/94‑1 (MQ=60)
                     GGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTC                                                                         >  SRR4302545.3305399/1‑94 (MQ=60)
                     GGAGCCAACTGCTTGGCGAAATCAGCGCAGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTC                                                                         <  SRR4302545.7217575/94‑1 (MQ=60)
                      GAGCCAACTGCTTGTCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCG                                                                        <  SRR4302545.1416841/94‑1 (MQ=60)
                      GAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCG                                                                        <  SRR4302545.7654772/94‑1 (MQ=60)
                       AGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGG                                                                       >  SRR4302545.3923857/1‑94 (MQ=60)
                        GCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGC                                                                      >  SRR4302545.4103298/1‑94 (MQ=60)
                        GCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGC                                                                      <  SRR4302545.9442136/94‑1 (MQ=60)
                         CCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCA                                                                     >  SRR4302545.3372407/1‑94 (MQ=60)
                         CCAACTGCTTGGCGAAATCAGCGAGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCA                                                                     <  SRR4302545.7417631/94‑1 (MQ=60)
                          CAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATAAAACCGGTTCGGCAG                                                                    >  SRR4302545.5502003/1‑94 (MQ=60)
                           AACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGT                                                                   >  SRR4302545.8882265/1‑94 (MQ=60)
                           AACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGT                                                                   <  SRR4302545.5205851/94‑1 (MQ=60)
                           AACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGT                                                                   >  SRR4302545.8942853/1‑94 (MQ=60)
                            ACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTC                                                                  <  SRR4302545.9978283/94‑1 (MQ=60)
                            ACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTC                                                                  <  SRR4302545.7051235/94‑1 (MQ=60)
                            ACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTC                                                                  <  SRR4302545.3625952/94‑1 (MQ=60)
                            ACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTC                                                                  <  SRR4302545.7897566/94‑1 (MQ=60)
                            ACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTC                                                                  <  SRR4302545.6121554/94‑1 (MQ=60)
                             ctgtggtGCGAATTCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGTGGAAAGTGATCTCGACAGAAAATACAtccgttttggttgtcg                                                                 >  SRR4302545.9684403/8‑78 (MQ=60)
                             CTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGCGATCTCGACAGAAAATACAACCGGTTCGGCAGTCG                                                                 >  SRR4302545.9848156/1‑94 (MQ=60)
                              TGTTTGGCGAATTCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGA                                                                >  SRR4302545.9625429/1‑94 (MQ=60)
                               GCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGAT                                                               >  SRR4302545.5243764/1‑94 (MQ=60)
                                CTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATG                                                              <  SRR4302545.3780600/94‑1 (MQ=60)
                                CTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATG                                                              <  SRR4302545.10725159/94‑1 (MQ=60)
                                CTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGGAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATG                                                              >  SRR4302545.6870024/1‑94 (MQ=60)
                                  TGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTT                                                            <  SRR4302545.1743296/94‑1 (MQ=60)
                                  TGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTT                                                            >  SRR4302545.9292241/1‑94 (MQ=60)
                                  TGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTT                                                            <  SRR4302545.6444748/94‑1 (MQ=60)
                                  TGGAGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTT                                                            >  SRR4302545.10266025/1‑94 (MQ=60)
                                    GCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCA                                                          <  SRR4302545.3612370/94‑1 (MQ=60)
                                    GCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCA                                                          <  SRR4302545.5108689/94‑1 (MQ=60)
                                     CGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAG                                                         >  SRR4302545.8223915/1‑94 (MQ=60)
                                     CGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAG                                                         <  SRR4302545.4038089/94‑1 (MQ=60)
                                      GAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGC                                                        <  SRR4302545.8528622/94‑1 (MQ=60)
                                      GAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGC                                                        >  SRR4302545.3437864/1‑94 (MQ=60)
                                      GAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGC                                                        <  SRR4302545.4623114/94‑1 (MQ=60)
                                       AAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCA                                                       <  SRR4302545.485001/94‑1 (MQ=60)
                                         ATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATG                                                     >  SRR4302545.5221226/1‑94 (MQ=60)
                                         ATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATG                                                     >  SRR4302545.4117736/1‑94 (MQ=60)
                                         ATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATG                                                     >  SRR4302545.5082579/1‑94 (MQ=60)
                                         ATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATG                                                     <  SRR4302545.8938927/94‑1 (MQ=60)
                                         ATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATG                                                     >  SRR4302545.2884136/1‑94 (MQ=60)
                                         ATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATG                                                     <  SRR4302545.6952058/94‑1 (MQ=60)
                                          TCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGA                                                    <  SRR4302545.3740561/94‑1 (MQ=60)
                                           acgctcttccgatctctgatcTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAG                                                   <  SRR4302545.4914606/73‑1 (MQ=60)
                                           CAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAG                                                   >  SRR4302545.10367228/1‑94 (MQ=60)
                                           CAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAG                                                   >  SRR4302545.3143591/1‑94 (MQ=60)
                                           AAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAG                                                   >  SRR4302545.1007853/1‑94 (MQ=60)
                                            AGCGCGGTAATGGGCACCGTTGGCAACGGCCTGCTGGGGCAAGTGATCTCGACAGAAAATACTACCGGTTCGGCAGTCGATGTTCAGCATGAGC                                                  >  SRR4302545.2512850/1‑94 (MQ=60)
                                             GCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCT                                                 <  SRR4302545.10307935/94‑1 (MQ=60)
                                              CGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTG                                                <  SRR4302545.3810197/94‑1 (MQ=60)
                                                CGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGG                                              >  SRR4302545.2753787/1‑94 (MQ=60)
                                                CGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGC                                              <  SRR4302545.8598196/94‑1 (MQ=60)
                                                 GGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCG                                             <  SRR4302545.6689354/94‑1 (MQ=60)
                                                  GTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGG                                            >  SRR4302545.2320482/1‑94 (MQ=60)
                                                  GTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGG                                            >  SRR4302545.7130425/1‑94 (MQ=60)
                                                  GGAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATCCAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGG                                            <  SRR4302545.6531885/94‑1 (MQ=60)
                                                   TAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGG                                           <  SRR4302545.420521/94‑1 (MQ=60)
                                                   TAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGG                                           <  SRR4302545.10897267/94‑1 (MQ=60)
                                                    AATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGG                                          <  SRR4302545.9046652/94‑1 (MQ=60)
                                                     ATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGC                                         <  SRR4302545.1728093/94‑1 (MQ=60)
                                                     ATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGAGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGC                                         >  SRR4302545.1065531/1‑94 (MQ=60)
                                                      TGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGGT                                        >  SRR4302545.8042416/1‑94 (MQ=60)
                                                      TGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCT                                        >  SRR4302545.6908611/1‑94 (MQ=60)
                                                      TGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCT                                        <  SRR4302545.8748270/94‑1 (MQ=60)
                                                      TGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCT                                        <  SRR4302545.2213869/94‑1 (MQ=60)
                                                      TGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGACCTGGCGGGGCG                                        >  SRR4302545.95326/1‑94 (MQ=60)
                                                       GGGCACCGTTGGTAACTGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTG                                       <  SRR4302545.6511249/94‑1 (MQ=60)
                                                       GGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTG                                       >  SRR4302545.8033117/1‑94 (MQ=60)
                                                       GGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTG                                       <  SRR4302545.219710/94‑1 (MQ=60)
                                                       GGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTG                                       <  SRR4302545.7047941/94‑1 (MQ=60)
                                                       GGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTG                                       >  SRR4302545.8033116/1‑94 (MQ=60)
                                                         GCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGT                                     <  SRR4302545.387415/94‑1 (MQ=60)
                                                         GCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGG                                     >  SRR4302545.5574660/1‑94 (MQ=60)
                                                         GCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGG                                     >  SRR4302545.2137495/1‑94 (MQ=60)
                                                           ACTGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGgctgtgg                                   >  SRR4302545.9622687/1‑87 (MQ=60)
                                                           ACCGTTGGTAACGGCCTGCTGGGGTAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGG                                   >  SRR4302545.5338817/1‑94 (MQ=60)
                                                           ACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGG                                   >  SRR4302545.2698943/1‑94 (MQ=60)
                                                             CGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGAT                                 >  SRR4302545.7186036/1‑94 (MQ=60)
                                                             CGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGAT                                 >  SRR4302545.4211521/1‑94 (MQ=60)
                                                             CGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGGGGAT                                 >  SRR4302545.3591821/1‑94 (MQ=60)
                                                              GTTGGTAACGGCCTGGTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATC                                <  SRR4302545.1870413/94‑1 (MQ=60)
                                                              GTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTTAGCATGAGCTGGCGGGGCTGGTGGATC                                >  SRR4302545.7246148/1‑94 (MQ=60)
                                                              GTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCgggggggtg                                >  SRR4302545.301312/1‑85 (MQ=60)
                                                              GTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATC                                >  SRR4302545.301311/1‑94 (MQ=60)
                                                               TTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGgtcg                               >  SRR4302545.2635561/1‑90 (MQ=60)
                                                               TTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCA                               <  SRR4302545.913265/94‑1 (MQ=60)
                                                               TTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCA                               <  SRR4302545.1973248/94‑1 (MQ=60)
                                                               TTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCA                               >  SRR4302545.2558912/1‑94 (MQ=60)
                                                                TGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAG                              <  SRR4302545.1190003/94‑1 (MQ=60)
                                                                TGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAG                              <  SRR4302545.6854361/94‑1 (MQ=60)
                                                                TGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAG                              <  SRR4302545.4022716/94‑1 (MQ=60)
                                                                TGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAG                              <  SRR4302545.10658736/94‑1 (MQ=60)
                                                                TGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAG                              <  SRR4302545.10086641/94‑1 (MQ=60)
                                                                 GGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACCACCGGTTCGGCAGTCGATGTTCAGAGTGAGCTGGCGGGGCatgaggaccacg                             >  SRR4302545.7505460/1‑82 (MQ=60)
                                                                 GGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGG                             <  SRR4302545.751447/94‑1 (MQ=60)
                                                                  GTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTTGCGGGGCTGGTGGggctggg                            >  SRR4302545.4342494/1‑87 (MQ=60)
                                                                  GTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGTGGGGCTGGTGGATCAGGG                            >  SRR4302545.9892964/1‑94 (MQ=60)
                                                                  GTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGG                            <  SRR4302545.7211748/94‑1 (MQ=60)
                                                                   TAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGC                           <  SRR4302545.3244961/94‑1 (MQ=60)
                                                                   GAACGGCCTGTTGGGGAAAGTGACCTCGACAGTAATTACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGC                           <  SRR4302545.4638983/94‑1 (MQ=60)
                                                                    AACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCG                          <  SRR4302545.8391115/94‑1 (MQ=60)
                                                                    AACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCG                          <  SRR4302545.8391114/94‑1 (MQ=60)
                                                                    AACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCG                          <  SRR4302545.8815148/94‑1 (MQ=60)
                                                                    AACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCG                          >  SRR4302545.7626687/1‑94 (MQ=60)
                                                                    AACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGGGGATCAGGGCG                          >  SRR4302545.2139493/1‑94 (MQ=60)
                                                                    AACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCGGGTGGggcacggcg                          >  SRR4302545.4295199/1‑85 (MQ=60)
                                                                      CGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGgccg                        >  SRR4302545.9905108/1‑90 (MQ=60)
                                                                      CGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCG                        >  SRR4302545.7048642/1‑94 (MQ=60)
                                                                      CGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCG                        >  SRR4302545.6091883/1‑94 (MQ=60)
                                                                       GGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGGTCAGGGCGCGA                       >  SRR4302545.9283165/1‑94 (MQ=60)
                                                                       GGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGA                       >  SRR4302545.7575637/1‑94 (MQ=60)
                                                                       GGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGGGGATCAGGGCGGGA                       >  SRR4302545.8197228/1‑94 (MQ=60)
                                                                         CCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACG                     <  SRR4302545.2660411/94‑1 (MQ=60)
                                                                         CCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACG                     <  SRR4302545.1504526/94‑1 (MQ=60)
                                                                          CTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGT                    <  SRR4302545.6591894/94‑1 (MQ=60)
                                                                          CTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGT                    >  SRR4302545.3414026/1‑94 (MQ=60)
                                                                           TGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTT                   >  SRR4302545.2247647/1‑94 (MQ=60)
                                                                            GCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTT                  <  SRR4302545.4545402/94‑1 (MQ=60)
                                                                             CTGGGTAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCTACGTTTT                 <  SRR4302545.10669239/94‑1 (MQ=60)
                                                                             CTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTT                 >  SRR4302545.1361866/1‑94 (MQ=60)
                                                                             CTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTT                 >  SRR4302545.1498129/1‑94 (MQ=60)
                                                                             CTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTT                 >  SRR4302545.7884977/1‑94 (MQ=60)
                                                                             CTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTT                 >  SRR4302545.392547/1‑94 (MQ=60)
                                                                             CTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGGTTT                 >  SRR4302545.210824/1‑94 (MQ=60)
                                                                              TGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTG                <  SRR4302545.1357893/94‑1 (MQ=60)
                                                                              TGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTG                >  SRR4302545.4637483/1‑94 (MQ=60)
                                                                              TGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTG                >  SRR4302545.9708319/1‑94 (MQ=60)
                                                                              TGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTG                >  SRR4302545.9708320/1‑94 (MQ=60)
                                                                              TGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTG                >  SRR4302545.5848760/1‑94 (MQ=60)
                                                                              TGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTG                <  SRR4302545.9147283/94‑1 (MQ=60)
                                                                              TGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGGTTTG                >  SRR4302545.5848761/1‑94 (MQ=60)
                                                                               GGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGG               >  SRR4302545.3796557/1‑94 (MQ=60)
                                                                                GGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGGTCAGGGggcggaggttttgg              >  SRR4302545.4432892/1‑80 (MQ=60)
                                                                                GGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGgttttgg              >  SRR4302545.9902121/1‑87 (MQ=60)
                                                                                GGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGAGCAGGGCGCGGCGTTTTGGG              >  SRR4302545.4432891/1‑94 (MQ=60)
                                                                                  GAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCA            <  SRR4302545.303411/94‑1 (MQ=60)
                                                                                   AAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAA           >  SRR4302545.7481408/1‑94 (MQ=60)
                                                                                   AAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGATGGCGGGGCTGGTGGATCAGGGCGGGACGTTTTGCGCAA           >  SRR4302545.7649499/1‑94 (MQ=60)
                                                                                    AAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAAT          <  SRR4302545.5187430/94‑1 (MQ=60)
                                                                                    AAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAAT          >  SRR4302545.5339175/1‑94 (MQ=60)
                                                                                     AGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCTGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATG         >  SRR4302545.10653100/1‑94 (MQ=60)
                                                                                     AGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTTCGCAATG         >  SRR4302545.1000231/1‑94 (MQ=60)
                                                                                     AGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATG         <  SRR4302545.6817354/94‑1 (MQ=60)
                                                                                     AGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATG         >  SRR4302545.4539982/1‑94 (MQ=60)
                                                                                     AGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATG         <  SRR4302545.9543721/94‑1 (MQ=60)
                                                                                     AGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATG         <  SRR4302545.10148510/94‑1 (MQ=60)
                                                                                      GTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGG        >  SRR4302545.7627384/1‑94 (MQ=60)
                                                                                      ATGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGG        <  SRR4302545.3298933/94‑1 (MQ=60)
                                                                                        GATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGTGCGACGTTTTGCGCAATGGAA      >  SRR4302545.9771283/1‑94 (MQ=60)
                                                                                         ATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAG     >  SRR4302545.10123397/1‑94 (MQ=60)
                                                                                         ATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAG     <  SRR4302545.7417875/94‑1 (MQ=60)
                                                                                         ATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAG     >  SRR4302545.9987735/1‑94 (MQ=60)
                                                                                          TCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGT    <  SRR4302545.3358675/94‑1 (MQ=60)
                                                                                          TCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGT    <  SRR4302545.3705356/94‑1 (MQ=60)
                                                                                          TCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGT    >  SRR4302545.496881/1‑94 (MQ=60)
                                                                                          TCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGT    <  SRR4302545.10386818/94‑1 (MQ=60)
                                                                                           CTCGACAGAAAATACAACCGGTTCGGCAGTCGATGATCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTT   >  SRR4302545.6868845/1‑94 (MQ=60)
                                                                                            TCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTT  <  SRR4302545.5490115/94‑1 (MQ=60)
                                                                                            |                                                                                             
CTACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTTT  >  NC_000913/93533‑93718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑