Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 93,625 C→T P154S (CCG→TCG)  murE → meso‑diaminopimelate‑adding enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091393,6250CT100.0% 815.6 / NA 228P154S (CCG→TCG) murEmeso‑diaminopimelate‑adding enzyme
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (115/113);  total (115/113)

Only 100 of 235 total aligned reads displayed.

ACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAA  >  NC_000913/93535‑93714
                                                                                          |                                                                                         
aCCCAGCTGTTGGCGCAGTGTAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCg                                                                                         <  1:1730645/93‑1 (MQ=255)
aCCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCg                                                                                         <  1:4727764/93‑1 (MQ=255)
 cccAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGa                                                                                        <  2:1448947/93‑1 (MQ=255)
 cccAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGa                                                                                        >  2:3544144/1‑93 (MQ=255)
  ccAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGCAAGTGATCTCGaa                                                                                       >  1:5576238/1‑92 (MQ=255)
   cAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACa                                                                                      >  1:6339312/1‑93 (MQ=255)
   cAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACa                                                                                      >  1:1452061/1‑93 (MQ=255)
    aGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAg                                                                                     >  2:2623212/1‑93 (MQ=255)
        gTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGaaaa                                                                                 >  1:1781169/1‑93 (MQ=255)
        gTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGaaaa                                                                                 <  1:4389599/93‑1 (MQ=255)
        gTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGaaaa                                                                                 <  2:6221399/93‑1 (MQ=255)
         ttGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAAt                                                                                <  1:5366783/93‑1 (MQ=255)
         ttGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAAt                                                                                >  1:2962740/1‑93 (MQ=255)
         ttGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAAt                                                                                >  1:403008/1‑93 (MQ=255)
         ttGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAAt                                                                                <  1:4713167/93‑1 (MQ=255)
          tGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATa                                                                               <  2:5762145/93‑1 (MQ=255)
          tGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATa                                                                               <  2:2965194/93‑1 (MQ=255)
           ggCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATAc                                                                              <  1:2152651/93‑1 (MQ=255)
           ggCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATAc                                                                              <  1:1840570/93‑1 (MQ=255)
            gcgcAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACa                                                                             <  2:1066826/93‑1 (MQ=255)
             cgcAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACaa                                                                            <  1:3553151/93‑1 (MQ=255)
             cgcAGTGGAGACAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACaa                                                                            <  1:670676/93‑1 (MQ=255)
              gcAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAAc                                                                           >  2:4396999/1‑93 (MQ=255)
                aGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCg                                                                         <  1:5734328/93‑1 (MQ=255)
                 gTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCgg                                                                        >  2:3797501/1‑93 (MQ=255)
                   ggAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGtt                                                                      <  1:2185359/93‑1 (MQ=255)
                      gCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCgg                                                                   >  2:3955731/1‑93 (MQ=255)
                        cAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGCAAGTGATCTCGACAGAAAATACAACCGGTTCGGCa                                                                 >  2:2572790/1‑93 (MQ=255)
                        cAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCa                                                                 >  2:5994453/1‑93 (MQ=255)
                        cAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCa                                                                 <  1:5308899/93‑1 (MQ=255)
                        cAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCa                                                                 <  2:1309778/93‑1 (MQ=255)
                        cAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCa                                                                 <  2:3110354/93‑1 (MQ=255)
                         aaCTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAg                                                                >  1:4172851/1‑93 (MQ=255)
                         aaCTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAg                                                                >  2:2506867/1‑93 (MQ=255)
                         aaCTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAg                                                                >  1:5404089/1‑93 (MQ=255)
                         aaCTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAg                                                                >  1:1207342/1‑93 (MQ=255)
                           cTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTc                                                              <  1:3926249/93‑1 (MQ=255)
                           cTGCTTGGCGAAATCAGCGCGGTAAGGGGCACCGTTGGTAACGGCCTGCGGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTc                                                              <  2:1615691/93‑1 (MQ=255)
                            tGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCg                                                             >  2:4871297/1‑93 (MQ=255)
                            tGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCg                                                             <  1:1994982/93‑1 (MQ=255)
                            ggCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCg                                                             <  2:1256626/92‑1 (MQ=255)
                             gCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGa                                                            <  1:4222291/93‑1 (MQ=255)
                             gCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGa                                                            <  1:2251841/93‑1 (MQ=255)
                              cTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGAt                                                           <  2:6098495/93‑1 (MQ=255)
                               ttGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATg                                                          <  1:1578954/93‑1 (MQ=255)
                               ttGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATg                                                          >  1:3032347/1‑93 (MQ=255)
                                 ggCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGtt                                                        <  2:2276503/93‑1 (MQ=255)
                                    gAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAg                                                     >  2:415112/1‑93 (MQ=255)
                                    gAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATATTCAg                                                     >  1:2044445/1‑93 (MQ=255)
                                     aaaTCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAga                                                    >  2:3282708/1‑92 (MQ=255)
                                      aaTCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCa                                                   <  1:5179717/93‑1 (MQ=255)
                                       aTCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCAt                                                  <  2:3378605/93‑1 (MQ=255)
                                        tCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATg                                                 <  1:4877632/93‑1 (MQ=255)
                                        tCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATg                                                 >  2:5501153/1‑93 (MQ=255)
                                         cAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGa                                                <  1:731804/93‑1 (MQ=255)
                                         cAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGATTCGGCAGTCGATGTTCAGCATGa                                                >  2:6349155/1‑93 (MQ=255)
                                          aGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAg                                               <  2:3390032/93‑1 (MQ=255)
                                           gcgcGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGc                                              >  2:736137/1‑93 (MQ=255)
                                             gcgGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTg                                            <  1:1083740/93‑1 (MQ=255)
                                              cgGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTgg                                           >  1:2721140/1‑93 (MQ=255)
                                              cgGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTgg                                           <  1:6040486/93‑1 (MQ=255)
                                              cgGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTgg                                           <  1:3769761/93‑1 (MQ=255)
                                              cgGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTgg                                           <  1:4461271/93‑1 (MQ=255)
                                                gTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCg                                         >  2:4994966/1‑93 (MQ=255)
                                                 tAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCgg                                        >  2:351110/1‑93 (MQ=255)
                                                  aaTGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCggg                                       >  2:823562/1‑93 (MQ=255)
                                                  aaTGGGCAACGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGCGCTGGCggg                                       >  2:6324300/1‑93 (MQ=255)
                                                   aTGGGCACCGTTGGTAACGGCCTGCTGGTGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCgggg                                      >  2:1173801/1‑93 (MQ=255)
                                                     gggCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCt                                    >  2:1022059/1‑93 (MQ=255)
                                                     gggCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCt                                    >  1:1164370/1‑93 (MQ=255)
                                                       gCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCtgg                                  <  2:2182905/93‑1 (MQ=255)
                                                        cACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCtggt                                 <  1:4579327/93‑1 (MQ=255)
                                                         aCCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGCGGCtggtg                                >  1:2851948/1‑93 (MQ=255)
                                                          ccGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCtggtgg                               >  1:762668/1‑93 (MQ=255)
                                                          ccGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCCGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCtggtgg                               <  1:3466308/93‑1 (MQ=255)
                                                           cGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGa                              <  1:1065617/93‑1 (MQ=255)
                                                            gTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCtggggggt                             >  2:5769567/1‑91 (MQ=255)
                                                            gTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGAt                             <  2:5182020/93‑1 (MQ=255)
                                                            gTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGAt                             <  1:5124995/93‑1 (MQ=255)
                                                             ttGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGGTc                            >  2:4124343/1‑93 (MQ=255)
                                                             ttGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATc                            >  2:3888063/1‑93 (MQ=255)
                                                              tGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCa                           >  2:3772665/1‑93 (MQ=255)
                                                               ggTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAg                          <  1:4959235/93‑1 (MQ=255)
                                                               ggTAACGGCCTGCCGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAg                          >  2:6356544/1‑93 (MQ=255)
                                                                 tAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAggg                        >  1:4917587/1‑93 (MQ=255)
                                                                      gCCTGCTGGGGAAAGTGATCTCGACAGAAAATACCACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGGGGGTCAGGGGGCGa                   >  2:3740874/1‑93 (MQ=255)
                                                                      gCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGGGGATCAGGGCGCGa                   >  1:2619348/1‑93 (MQ=255)
                                                                        ctgctgGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACg                 >  1:3666112/1‑93 (MQ=255)
                                                                        ctgctgGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACg                 >  2:2610300/1‑93 (MQ=255)
                                                                           ctgGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGttt              >  2:1654753/1‑93 (MQ=255)
                                                                            tgGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGtttt             <  2:5150825/93‑1 (MQ=255)
                                                                            tgGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGtttt             >  1:4125516/1‑93 (MQ=255)
                                                                            tgGGGAAAGTGATCTCGACAGAAAATACAACCCGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGAGCTCGTGGAGCAGGGCGCGCTGtttt             >  2:3671399/1‑93 (MQ=255)
                                                                                gAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTgcgc         >  2:2743054/1‑93 (MQ=255)
                                                                                 aaaGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCa        >  1:3258134/1‑93 (MQ=255)
                                                                                     tGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATgg    >  1:2468370/1‑93 (MQ=255)
                                                                                      gATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGCTTTGCGCAATGGa   >  2:517510/1‑93 (MQ=255)
                                                                                       aTCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGaa  >  2:582350/1‑93 (MQ=255)
                                                                                       aTCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGaa  >  1:6245001/1‑93 (MQ=255)
                                                                                       aTCTCGACAGAAAATACAACCGGTTCGGCAGGCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGaa  >  1:5957011/1‑93 (MQ=255)
                                                                                          |                                                                                         
ACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAA  >  NC_000913/93535‑93714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTT  >  NC_000913/93532‑93717
                                                                                             |                                                                                            
ACTACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCT                                                                                              <  SRR4302537.3988173/94‑1 (MQ=60)
 CTACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTC                                                                                             <  SRR4302537.5391017/94‑1 (MQ=60)
  TACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCG                                                                                            <  SRR4302537.4102387/94‑1 (MQ=60)
  TACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCG                                                                                            <  SRR4302537.430063/94‑1 (MQ=60)
  TACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCG                                                                                            <  SRR4302537.2457085/94‑1 (MQ=60)
  TACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCG                                                                                            <  SRR4302537.275463/94‑1 (MQ=60)
   ACCCAGCTGTTGGCGCAGTGTAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGA                                                                                           <  SRR4302537.1742725/94‑1 (MQ=60)
   ACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGA                                                                                           <  SRR4302537.3581969/94‑1 (MQ=60)
   ACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGA                                                                                           <  SRR4302537.4760442/94‑1 (MQ=60)
    CCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGAC                                                                                          >  SRR4302537.1369999/1‑94 (MQ=60)
     CCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACA                                                                                         >  SRR4302537.1462179/1‑94 (MQ=60)
     CCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACA                                                                                         >  SRR4302537.6382631/1‑94 (MQ=60)
      CAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAG                                                                                        <  SRR4302537.2590029/94‑1 (MQ=60)
       CGCTGTTGGCGGAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATTGGCCCCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGA                                                                                       <  SRR4302537.4213098/94‑1 (MQ=60)
        GCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAA                                                                                      <  SRR4302537.4842323/94‑1 (MQ=60)
          TGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAA                                                                                    <  SRR4302537.6032904/94‑1 (MQ=60)
          TGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAA                                                                                    >  SRR4302537.1793582/1‑94 (MQ=60)
           GTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAAT                                                                                   >  SRR4302537.2983537/1‑94 (MQ=60)
           GTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAAT                                                                                   >  SRR4302537.6474512/1‑94 (MQ=60)
           GTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAAT                                                                                   <  SRR4302537.168039/94‑1 (MQ=60)
           GTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAAT                                                                                   >  SRR4302537.405801/1‑94 (MQ=60)
           GTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAAT                                                                                   <  SRR4302537.4419870/94‑1 (MQ=60)
            TTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATA                                                                                  <  SRR4302537.4745769/94‑1 (MQ=60)
            TTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATA                                                                                  <  SRR4302537.5403505/94‑1 (MQ=60)
             TGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATAC                                                                                 <  SRR4302537.5322708/94‑1 (MQ=60)
              GGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACA                                                                                <  SRR4302537.1853375/94‑1 (MQ=60)
              GGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACA                                                                                <  SRR4302537.2167826/94‑1 (MQ=60)
              GGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACA                                                                                <  SRR4302537.4556427/94‑1 (MQ=60)
                CGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAAC                                                                              <  SRR4302537.4109273/94‑1 (MQ=60)
                CGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAAC                                                                              >  SRR4302537.3652875/1‑94 (MQ=60)
                CGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAAC                                                                              <  SRR4302537.3578236/94‑1 (MQ=60)
                CGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAAC                                                                              <  SRR4302537.6217500/94‑1 (MQ=60)
                CGCAGTGGAGACAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAAC                                                                              <  SRR4302537.675260/94‑1 (MQ=60)
                 GCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACC                                                                             <  SRR4302537.5413695/94‑1 (MQ=60)
                  CAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCG                                                                            <  SRR4302537.6054416/94‑1 (MQ=60)
                   AGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGG                                                                           >  SRR4302537.1073880/1‑94 (MQ=60)
                   AGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGG                                                                           <  SRR4302537.5773588/94‑1 (MQ=60)
                   AGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGG                                                                           >  SRR4302537.1852423/1‑94 (MQ=60)
                     TGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTT                                                                         <  SRR4302537.1947030/94‑1 (MQ=60)
                     TGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTT                                                                         <  SRR4302537.5718109/94‑1 (MQ=60)
                      GGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGCCAGAAAATACAACCGctac                                                                        >  SRR4302537.2187381/1‑90 (MQ=60)
                      GGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTC                                                                        <  SRR4302537.2200757/94‑1 (MQ=60)
                      GGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTC                                                                        >  SRR4302537.1579036/1‑94 (MQ=60)
                        AGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGG                                                                      <  SRR4302537.2244387/94‑1 (MQ=60)
                          CCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCA                                                                    <  SRR4302537.3560160/94‑1 (MQ=60)
                           CAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAG                                                                   <  SRR4302537.5345373/94‑1 (MQ=60)
                           CAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAG                                                                   >  SRR4302537.4201994/1‑94 (MQ=60)
                           CAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAG                                                                   >  SRR4302537.5440960/1‑94 (MQ=60)
                           CAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAG                                                                   >  SRR4302537.3783124/1‑94 (MQ=60)
                           CAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAG                                                                   >  SRR4302537.1215745/1‑94 (MQ=60)
                             ACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTC                                                                 <  SRR4302537.4167212/94‑1 (MQ=60)
                              CTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCG                                                                <  SRR4302537.3953680/94‑1 (MQ=60)
                               TGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGA                                                               <  SRR4302537.2008852/94‑1 (MQ=60)
                               TGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGA                                                               >  SRR4302537.5282128/1‑94 (MQ=60)
                                GCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGAT                                                              >  SRR4302537.2668786/1‑94 (MQ=60)
                                GCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGAT                                                              <  SRR4302537.2267738/94‑1 (MQ=60)
                                GCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGAT                                                              <  SRR4302537.4251702/94‑1 (MQ=60)
                                GCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGAT                                                              <  SRR4302537.761955/94‑1 (MQ=60)
                                 CTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATG                                                             >  SRR4302537.3053490/1‑94 (MQ=60)
                                 CTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATG                                                             <  SRR4302537.2848802/94‑1 (MQ=60)
                                  TTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGT                                                            <  SRR4302537.1589988/94‑1 (MQ=60)
                                   TGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTT                                                           >  SRR4302537.4392514/1‑94 (MQ=60)
                                      CGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATATTCAG                                                        >  SRR4302537.2058763/1‑94 (MQ=60)
                                        AAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCA                                                      <  SRR4302537.712945/94‑1 (MQ=60)
                                         AATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCAT                                                     <  SRR4302537.5215362/94‑1 (MQ=60)
                                           TCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGA                                                   <  SRR4302537.4911329/94‑1 (MQ=60)
                                            CAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAG                                                  <  SRR4302537.736799/94‑1 (MQ=60)
                                            CAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAG                                                  <  SRR4302537.5026223/94‑1 (MQ=60)
                                             AGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGC                                                 <  SRR4302537.1731981/94‑1 (MQ=60)
                                                GCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGG                                              <  SRR4302537.1091218/94‑1 (MQ=60)
                                                GCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGG                                              >  SRR4302537.2740142/1‑94 (MQ=60)
                                                 CGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGC                                             <  SRR4302537.2193401/94‑1 (MQ=60)
                                                 CGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGC                                             >  SRR4302537.799127/1‑94 (MQ=60)
                                                 CGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGC                                             <  SRR4302537.6081984/94‑1 (MQ=60)
                                                 CGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGC                                             <  SRR4302537.4492113/94‑1 (MQ=60)
                                                 CGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGC                                             <  SRR4302537.3796228/94‑1 (MQ=60)
                                                  GGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATGCAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCG                                            >  SRR4302537.2228150/1‑94 (MQ=60)
                                                  GGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCG                                            >  SRR4302537.5985363/1‑94 (MQ=60)
                                                  GGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTCACGCATGGGCTGGCG                                            >  SRR4302537.6346993/1‑94 (MQ=60)
                                                    TAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGG                                          >  SRR4302537.3431556/1‑94 (MQ=60)
                                                    TAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGG                                          <  SRR4302537.1183627/94‑1 (MQ=60)
                                                     AATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGG                                         >  SRR4302537.384147/1‑94 (MQ=60)
                                                      ATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGC                                        >  SRR4302537.2119997/1‑94 (MQ=60)
                                                       TGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCT                                       >  SRR4302537.1172464/1‑94 (MQ=60)
                                                       TGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCT                                       <  SRR4302537.3969887/94‑1 (MQ=60)
                                                       TGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCT                                       <  SRR4302537.3969888/94‑1 (MQ=60)
                                                           CACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTG                                   <  SRR4302537.4611008/94‑1 (MQ=60)
                                                           CACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGCGGCTGGTG                                   >  SRR4302537.2871929/1‑94 (MQ=60)
                                                            ACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGG                                  >  SRR4302537.767878/1‑94 (MQ=60)
                                                             CCGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCCGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGA                                 <  SRR4302537.3490822/94‑1 (MQ=60)
                                                              CGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACACCCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGAT                                <  SRR4302537.4289095/94‑1 (MQ=60)
                                                              CGTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGAT                                <  SRR4302537.1072956/94‑1 (MQ=60)
                                                               GTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGCTC                               >  SRR4302537.2391862/1‑94 (MQ=60)
                                                               GTTGGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATC                               <  SRR4302537.5160314/94‑1 (MQ=60)
                                                                  GGTAACGGCGTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGG                            <  SRR4302537.4557938/94‑1 (MQ=60)
                                                                  GGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGG                            <  SRR4302537.5172219/94‑1 (MQ=60)
                                                                  GGTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGG                            <  SRR4302537.4993420/94‑1 (MQ=60)
                                                                   GTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATCCAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGG                           <  SRR4302537.1889075/94‑1 (MQ=60)
                                                                   GTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTTGATCAGGG                           >  SRR4302537.3129628/1‑94 (MQ=60)
                                                                   GTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGG                           >  SRR4302537.4977368/1‑94 (MQ=60)
                                                                   GTAACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGG                           >  SRR4302537.4951514/1‑94 (MQ=60)
                                                                      ACGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGC                        <  SRR4302537.3006007/94‑1 (MQ=60)
                                                                       CGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCG                       <  SRR4302537.4938306/94‑1 (MQ=60)
                                                                       CGGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCG                       <  SRR4302537.1094299/94‑1 (MQ=60)
                                                                        GGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGA                      >  SRR4302537.5367582/1‑94 (MQ=60)
                                                                        GGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGA                      >  SRR4302537.4233127/1‑94 (MQ=60)
                                                                        GGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGA                      >  SRR4302537.2538029/1‑94 (MQ=60)
                                                                        GGCCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGGGGATCAGGGCGCGA                      >  SRR4302537.2637658/1‑94 (MQ=60)
                                                                          CCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACG                    >  SRR4302537.5488239/1‑94 (MQ=60)
                                                                          CCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACG                    >  SRR4302537.3691915/1‑94 (MQ=60)
                                                                          CCTGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACG                    >  SRR4302537.2037380/1‑94 (MQ=60)
                                                                            TGCTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTGCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTT                  <  SRR4302537.991854/94‑1 (MQ=60)
                                                                              CTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTT                >  SRR4302537.476859/1‑94 (MQ=60)
                                                                              CTGGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTT                >  SRR4302537.4154310/1‑94 (MQ=60)
                                                                                GGGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGGGGGGCAGGGGGCGACGTTTTGG              >  SRR4302537.4748554/1‑94 (MQ=60)
                                                                                 GGGAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGGCGTTTTGCG             >  SRR4302537.1774000/1‑94 (MQ=60)
                                                                                   GAAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCA           >  SRR4302537.3281104/1‑94 (MQ=60)
                                                                                    AAAGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGGTTTGCGCCA          >  SRR4302537.5542001/1‑94 (MQ=60)
                                                                                      AGTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAAGGCGCGACGGTTTGCGCAcag        >  SRR4302537.5155405/1‑91 (MQ=60)
                                                                                       GTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGTCGCGACGTTTTGCGCAATGG       <  SRR4302537.2940312/94‑1 (MQ=60)
                                                                                       GTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGG       >  SRR4302537.2485724/1‑94 (MQ=60)
                                                                                       GTGATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGG       >  SRR4302537.672533/1‑94 (MQ=60)
                                                                                         GATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAA     >  SRR4302537.6287917/1‑94 (MQ=60)
                                                                                         GATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAA     >  SRR4302537.3201091/1‑94 (MQ=60)
                                                                                         GATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAA     >  SRR4302537.4662719/1‑94 (MQ=60)
                                                                                         GATCTCGACAGAAAATACAACCGGTTCGGCAGGCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAA     >  SRR4302537.5997900/1‑94 (MQ=60)
                                                                                          ATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAG    <  SRR4302537.4431723/94‑1 (MQ=60)
                                                                                          ATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAG    <  SRR4302537.2879221/94‑1 (MQ=60)
                                                                                          ATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAG    >  SRR4302537.409807/1‑94 (MQ=60)
                                                                                          ATCTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAG    >  SRR4302537.602630/1‑94 (MQ=60)
                                                                                            CTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTT  >  SRR4302537.1659731/1‑94 (MQ=60)
                                                                                            CTCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTT  >  SRR4302537.3032385/1‑94 (MQ=60)
                                                                                             |                                                                                            
ACTACCCAGCTGTTGGCGCAGTGGAGCCAACTGCTTGGCGAAATCAGCGCGGTAATGGGCACCGTTGGTAACGGCCTGCTGGGGAAAGTGATCCCGACAGAAAATACAACCGGTTCGGCAGTCGATGTTCAGCATGAGCTGGCGGGGCTGGTGGATCAGGGCGCGACGTTTTGCGCAATGGAAGTT  >  NC_000913/93532‑93717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑