Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 187,433 A→G H406H (CAT→CAC)  glnD ← protein PII

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913187,4330AG99.2% 1308.1 / ‑4.7 381H406H (CAT→CAC) glnDprotein PII
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base G (169/208);  total (171/210)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 387 total aligned reads displayed.

GCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAG  >  NC_000913/187342‑187523
                                                                                           |                                                                                          
gCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTg                                                                                          <  2:10044898/94‑1 (MQ=255)
   aTAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCg                                                                                       <  1:9809254/94‑1 (MQ=255)
     aGCTCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGAc                                                                                     <  1:10347871/94‑1 (MQ=255)
     aGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACGCAGCGGTTGTTGCAGGTGGCGAc                                                                                     >  1:5876279/1‑94 (MQ=255)
     aGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGAc                                                                                     <  1:10549348/94‑1 (MQ=255)
       cccGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACgg                                                                                   <  2:10363653/94‑1 (MQ=255)
        ccGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACggg                                                                                  <  2:9737281/94‑1 (MQ=255)
          gcgcCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCa                                                                                >  2:9526996/1‑94 (MQ=255)
            gccgcACCGCTCCGGGGTGACGCAGAATGCTCAAAACCAGTTTTCGTGCTTCCGGAATATTTCACAGCGGTTGTTGCAGGTGGCGACGGGCaag                                                                              <  1:8637001/94‑3 (MQ=255)
            gccgcACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATg                                                                              >  1:10039617/1‑94 (MQ=255)
             ccgcACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATgg                                                                             <  2:4099754/94‑1 (MQ=255)
             ccgcACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATgg                                                                             >  2:661842/1‑94 (MQ=255)
              cgcACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGc                                                                            <  2:165935/94‑1 (MQ=255)
              cgcACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGc                                                                            <  2:8444315/94‑1 (MQ=255)
              cgcACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGc                                                                            >  1:3608506/1‑94 (MQ=255)
               gcACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCg                                                                           >  2:5949867/1‑94 (MQ=255)
                cACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGt                                                                          >  2:7748161/1‑94 (MQ=255)
                cACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGt                                                                          <  2:4227637/94‑1 (MQ=255)
                cACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGt                                                                          >  1:282481/1‑94 (MQ=255)
                 aCCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTa                                                                         >  1:7380268/1‑94 (MQ=255)
                 aCCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTa                                                                         >  2:9313353/1‑94 (MQ=255)
                 aCCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTa                                                                         >  1:3543230/1‑94 (MQ=255)
                 aCCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTa                                                                         <  1:101173/94‑1 (MQ=255)
                    gCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACt                                                                      <  1:3902426/94‑1 (MQ=255)
                    gCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACt                                                                      <  2:3193816/94‑1 (MQ=255)
                       ccGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGgc                                                                   <  2:3018596/94‑1 (MQ=255)
                       ccGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGgc                                                                   <  1:6909451/94‑1 (MQ=255)
                       ccGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGgc                                                                   <  1:10627633/94‑1 (MQ=255)
                        cGGGGGGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGgcg                                                                  >  2:6137611/1‑94 (MQ=255)
                         ggggTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGgcgc                                                                 >  2:8214746/1‑94 (MQ=255)
                          gggTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCa                                                                <  1:6488396/94‑1 (MQ=255)
                           ggTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAg                                                               <  2:1453941/94‑1 (MQ=255)
                           ggTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAg                                                               >  2:10535944/1‑94 (MQ=255)
                            gTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGc                                                              <  2:230047/94‑1 (MQ=255)
                              gACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCgt                                                            <  2:7056629/94‑1 (MQ=255)
                               aCGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCgtg                                                           <  1:549016/94‑1 (MQ=255)
                               aCGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCgtg                                                           <  1:7559945/94‑1 (MQ=255)
                                cGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCgtgg                                                          <  1:4126586/94‑1 (MQ=255)
                                  cAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCgtggtg                                                        >  1:5646411/1‑94 (MQ=255)
                                   aGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGtggtgg                                                       <  2:2014174/94‑1 (MQ=255)
                                   aGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGtggtgg                                                       <  2:7442541/94‑1 (MQ=255)
                                    gAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGa                                                      >  2:4338667/1‑94 (MQ=255)
                                     aaTGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAg                                                     <  2:2494790/94‑1 (MQ=255)
                                      aTGCTCAAAGAACGTTTGTGTGCTTCAGGAAGATTACATAGCGGTTATTGCAGGTGGCGACGGGCATGGCGGAACTGGCGCAGCGTGGTGGAGt                                                    <  2:2961810/94‑1 (MQ=255)
                                      aTGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAga                                                    >  2:1759590/1‑93 (MQ=255)
                                           cAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATg                                               >  2:8793839/1‑94 (MQ=255)
                                            aaaaaCAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGc                                              <  1:4735590/94‑1 (MQ=255)
                                            aaaaaCAGTTTTCGTGCTTCCGGAATATTACACAGCGGGTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGc                                              >  1:10442417/1‑94 (MQ=255)
                                              aaaCAGTTTTCGTGCTTCCGGAATATTACGCAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCg                                            <  2:5764347/94‑1 (MQ=255)
                                              aaaCAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCg                                            >  1:102006/1‑94 (MQ=255)
                                               aaCAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCgg                                           >  1:6039764/1‑94 (MQ=255)
                                               aaCAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCgg                                           >  1:5336150/1‑94 (MQ=255)
                                               aaCAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGCGGAGTAAATGCCgg                                           >  1:8097087/1‑94 (MQ=255)
                                                aCAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGt                                          <  1:2984103/94‑1 (MQ=255)
                                                 cAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTg                                         <  1:4814878/94‑1 (MQ=255)
                                                 cAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTg                                         <  1:9130872/94‑1 (MQ=255)
                                                 cAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTg                                         <  2:7960831/94‑1 (MQ=255)
                                                   gTTTTCGTGCTTCCGGAATATTAAACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGAt                                       <  1:5851610/94‑1 (MQ=255)
                                                     tttCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCg                                     <  2:2599801/94‑1 (MQ=255)
                                                        cGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCAc                                  >  2:5058734/1‑94 (MQ=255)
                                                        cGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCAc                                  <  1:3306987/94‑1 (MQ=255)
                                                        cGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGCGGGGACGGGCATGGCGAAACTGGCGCAGCGCGGTGGAGTAAATGCCGGTGATCGCAc                                  >  2:5045573/1‑94 (MQ=255)
                                                         gTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGTCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACt                                 <  1:6690114/94‑1 (MQ=255)
                                                         gTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACt                                 <  1:7825417/94‑1 (MQ=255)
                                                         gTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACt                                 <  1:2914349/94‑1 (MQ=255)
                                                          tGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACtg                                <  1:2082438/94‑1 (MQ=255)
                                                           gCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACtgt                               <  2:2828065/94‑1 (MQ=255)
                                                            gttCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGAAGTAAATGCCGGTGATCGCACtgtt                              <  1:1427828/93‑1 (MQ=255)
                                                            cTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACtgtt                              <  1:9324454/94‑1 (MQ=255)
                                                            cTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGAGGTGGAGTAAATGCCGGTGATCGCACtgtt                              >  1:7216270/1‑94 (MQ=255)
                                                             ttCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACtgttg                             >  1:6097714/1‑94 (MQ=255)
                                                              tCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACtgttgt                            <  1:5236810/94‑1 (MQ=255)
                                                                 tgAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCTGTGATCGCACTGTTGTGCa                         <  1:8412062/93‑1 (MQ=255)
                                                                 ggAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCa                         <  1:434240/94‑1 (MQ=255)
                                                                 ggAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCa                         >  2:1252867/1‑94 (MQ=255)
                                                                   aaTATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCAcc                       <  1:8814230/94‑1 (MQ=255)
                                                                        tACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGgt                  <  1:653358/94‑1 (MQ=255)
                                                                         acacAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGgtg                 <  2:365118/94‑1 (MQ=255)
                                                                         acacAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGgtg                 >  2:4136806/1‑94 (MQ=255)
                                                                          cacaGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGgtgt                >  2:90129/1‑94 (MQ=255)
                                                                          cacaGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGgtgt                <  1:9513992/94‑1 (MQ=255)
                                                                           acaGCGTTTGTTGCAGGTGGCGACGAGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTa               <  2:10306615/94‑1 (MQ=255)
                                                                            caGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCCGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAg              >  2:8022477/1‑94 (MQ=255)
                                                                            caGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAg              <  1:1277377/94‑1 (MQ=255)
                                                                            caGCGGTTGTTGCAGGGGGCGACGGGCATGGCGCAACTGGCGCAGCGTGGTGGAGTAAATGCACGTGATCGCACTGTTGTGCACCATGGTGTAg              >  2:5357917/1‑94 (MQ=255)
                                                                             aGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGa             <  1:8281694/94‑1 (MQ=255)
                                                                               cGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAAc           >  1:636150/1‑94 (MQ=255)
                                                                                 gttgttGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGGGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGCACAt         >  2:7244895/1‑94 (MQ=255)
                                                                                 gttgttGCAGGCGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACAt         >  1:1771883/1‑94 (MQ=255)
                                                                                  ttgttgCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATa        <  2:7600634/94‑1 (MQ=255)
                                                                                  ttgttgCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATa        >  2:6407120/1‑94 (MQ=255)
                                                                                  ttgttgCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATa        <  2:3891721/94‑1 (MQ=255)
                                                                                   tgttgCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATAc       >  2:3322359/1‑94 (MQ=255)
                                                                                    gttgCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACg      <  1:8979006/94‑1 (MQ=255)
                                                                                    gttgCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACg      >  2:3051623/1‑94 (MQ=255)
                                                                                      tgCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCa    >  1:5010855/1‑94 (MQ=255)
                                                                                      tgCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCa    <  2:8509996/94‑1 (MQ=255)
                                                                                       gCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCaa   >  2:264063/1‑94 (MQ=255)
                                                                                        cAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAg  <  2:4679445/94‑1 (MQ=255)
                                                                                        cAGGTGGCGACGGGCATAGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAg  <  2:9397217/94‑1 (MQ=255)
                                                                                           |                                                                                          
GCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAG  >  NC_000913/187342‑187523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 209 total aligned reads displayed.
TGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATG  >  NC_000913/187340‑187526
                                                                                             |                                                                                             
TGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGG                                                                                               >  SRR4302545.5244127/1‑94 (MQ=60)
TGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGG                                                                                               <  SRR4302545.1754351/94‑1 (MQ=60)
TGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGG                                                                                               <  SRR4302545.3864360/94‑1 (MQ=60)
 GGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGT                                                                                              <  SRR4302545.5506544/94‑1 (MQ=60)
  GCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTG                                                                                             <  SRR4302545.8089061/94‑1 (MQ=60)
  GCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATAATACACAGCGGTTGTTGCAGGTG                                                                                             >  SRR4302545.1163005/1‑94 (MQ=60)
   CAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGG                                                                                            >  SRR4302545.8079773/1‑94 (MQ=60)
   CAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGG                                                                                            <  SRR4302545.406694/94‑1 (MQ=60)
    AATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGC                                                                                           >  SRR4302545.5461394/1‑94 (MQ=60)
     ATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCG                                                                                          >  SRR4302545.4306484/1‑94 (MQ=60)
     ATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCG                                                                                          <  SRR4302545.10100724/94‑1 (MQ=60)
       AGCTCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGAC                                                                                        <  SRR4302545.10652884/94‑1 (MQ=60)
       AGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACGCAGCGGTTGTTGCAGGTGGCGAC                                                                                        >  SRR4302545.6034666/1‑94 (MQ=60)
       AGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGAC                                                                                        <  SRR4302545.1715992/94‑1 (MQ=60)
       AGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGAC                                                                                        <  SRR4302545.7392960/94‑1 (MQ=60)
       AGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGAC                                                                                        <  SRR4302545.10652883/94‑1 (MQ=60)
       AGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGAC                                                                                        <  SRR4302545.10860084/94‑1 (MQ=60)
         CCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGG                                                                                      <  SRR4302545.1333643/94‑1 (MQ=60)
          CCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGG                                                                                     <  SRR4302545.8917121/94‑1 (MQ=60)
          CCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGG                                                                                     >  SRR4302545.1901302/1‑94 (MQ=60)
          CCGCGCCGCACCGCGCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGG                                                                                     <  SRR4302545.320784/94‑1 (MQ=60)
           CGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGC                                                                                    <  SRR4302545.6233068/94‑1 (MQ=60)
            GCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCA                                                                                   <  SRR4302545.5356092/94‑1 (MQ=60)
              GCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAACCAGTTTTCGTGCTTCCGGAATATTTCACAGCGGTTGTTGCAGGTGGCGACGGGCAAG                                                                                 <  SRR4302545.8887477/94‑1 (MQ=60)
              GCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATG                                                                                 >  SRR4302545.10336953/1‑94 (MQ=60)
               CCGCATCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGG                                                                                <  SRR4302545.3592502/94‑1 (MQ=60)
               CCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGG                                                                                >  SRR4302545.798132/1‑94 (MQ=60)
                CGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGC                                                                               >  SRR4302545.3696474/1‑94 (MQ=60)
                 GCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCG                                                                              >  SRR4302545.564628/1‑94 (MQ=60)
                  CACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGT                                                                             >  SRR4302545.288374/1‑94 (MQ=60)
                   ACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTA                                                                            >  SRR4302545.7588432/1‑94 (MQ=60)
                   ACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTA                                                                            <  SRR4302545.103507/94‑1 (MQ=60)
                   ACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTA                                                                            >  SRR4302545.3629949/1‑94 (MQ=60)
                     CGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAAC                                                                          >  SRR4302545.3691342/1‑94 (MQ=60)
                      GCTCCGGGGTGGCGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACT                                                                         <  SRR4302545.7075969/94‑1 (MQ=60)
                      GCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACT                                                                         <  SRR4302545.3999818/94‑1 (MQ=60)
                       CTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGTTGGCGACGGGCATGGCGTAACTG                                                                        >  SRR4302545.6019493/1‑94 (MQ=60)
                       CTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTG                                                                        >  SRR4302545.5985854/1‑94 (MQ=60)
                       CTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTG                                                                        >  SRR4302545.10780894/1‑94 (MQ=60)
                        TCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGG                                                                       <  SRR4302545.6227539/94‑1 (MQ=60)
                         CCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGC                                                                      <  SRR4302545.5404239/94‑1 (MQ=60)
                         CCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGC                                                                      <  SRR4302545.7099813/94‑1 (MQ=60)
                         CCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGC                                                                      <  SRR4302545.4886904/94‑1 (MQ=60)
                         CCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGC                                                                      <  SRR4302545.10940472/94‑1 (MQ=60)
                          CGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCG                                                                     >  SRR4302545.4672414/1‑94 (MQ=60)
                          CGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCG                                                                     >  SRR4302545.10607180/1‑94 (MQ=60)
                           GGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGC                                                                    >  SRR4302545.7686628/1‑94 (MQ=60)
                           GGGGTGACGCAGAATGCTCAAAAACAGTTTGCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGC                                                                    >  SRR4302545.6707692/1‑94 (MQ=60)
                            GGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCA                                                                   <  SRR4302545.6665809/94‑1 (MQ=60)
                             GGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAG                                                                  >  SRR4302545.2327725/1‑94 (MQ=60)
                             GGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAG                                                                  <  SRR4302545.4862554/94‑1 (MQ=60)
                             GGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAG                                                                  <  SRR4302545.6060902/94‑1 (MQ=60)
                               TGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCG                                                                <  SRR4302545.9266121/94‑1 (MQ=60)
                                 ACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTG                                                              <  SRR4302545.5550661/94‑1 (MQ=60)
                                 ACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTG                                                              <  SRR4302545.561283/94‑1 (MQ=60)
                                  CGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGAGGGGCATGGCGTAACTGGCGCAGCGTGG                                                             <  SRR4302545.10665529/94‑1 (MQ=60)
                                  CGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGG                                                             >  SRR4302545.5583696/1‑94 (MQ=60)
                                  CGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGG                                                             <  SRR4302545.4229790/94‑1 (MQ=60)
                                   gctgtatgctcttAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGGTGCAGGTTGCGACGGGCATGGCGTAACTGGCGCAGCGTGGG                                                            >  SRR4302545.9625051/14‑94 (MQ=60)
                                   GCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGG                                                            >  SRR4302545.7600317/1‑94 (MQ=60)
                                   GCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGG                                                            >  SRR4302545.8815806/1‑94 (MQ=60)
                                    CAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTG                                                           >  SRR4302545.5796780/1‑94 (MQ=60)
                                    CAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTG                                                           <  SRR4302545.5667186/94‑1 (MQ=60)
                                    CAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTG                                                           <  SRR4302545.10774138/94‑1 (MQ=60)
                                     AGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGG                                                          >  SRR4302545.3200712/1‑94 (MQ=60)
                                     AGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGG                                                          >  SRR4302545.4267819/1‑94 (MQ=60)
                                     AGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGGGG                                                          >  SRR4302545.8438317/1‑94 (MQ=60)
                                      GAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGG                                                         >  SRR4302545.3626385/1‑94 (MQ=60)
                                      GAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGA                                                         >  SRR4302545.6556910/1‑94 (MQ=60)
                                      GAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGA                                                         <  SRR4302545.1747254/94‑1 (MQ=60)
                                      GAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGA                                                         >  SRR4302545.4705576/1‑94 (MQ=60)
                                       AATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAG                                                        <  SRR4302545.9370349/94‑1 (MQ=60)
                                         TGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTA                                                      <  SRR4302545.1799153/94‑1 (MQ=60)
                                         TGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCACCGTGGTGGAGTA                                                      >  SRR4302545.9096528/1‑94 (MQ=60)
                                          cttccgatctctgatctGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAA                                                     >  SRR4302545.9786907/18‑94 (MQ=60)
                                          cttccgatctctgatctGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAA                                                     >  SRR4302545.9786908/18‑94 (MQ=60)
                                          GCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAA                                                     >  SRR4302545.6748043/1‑94 (MQ=60)
                                           CTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAA                                                    >  SRR4302545.970621/1‑94 (MQ=60)
                                           CTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAA                                                    >  SRR4302545.10669677/1‑94 (MQ=60)
                                            TCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAAT                                                   <  SRR4302545.3782925/94‑1 (MQ=60)
                                            TCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAAT                                                   <  SRR4302545.7544717/94‑1 (MQ=60)
                                            TCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAAT                                                   <  SRR4302545.6569228/94‑1 (MQ=60)
                                            CCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTACCTGGCGCAGCGTGGTGGAGTAAAT                                                   <  SRR4302545.3473370/94‑1 (MQ=60)
                                             CAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATG                                                  >  SRR4302545.10062127/1‑94 (MQ=60)
                                             CAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATG                                                  >  SRR4302545.412869/1‑94 (MQ=60)
                                             CAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATG                                                  >  SRR4302545.7234493/1‑94 (MQ=60)
                                             CAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATG                                                  >  SRR4302545.2916994/1‑94 (MQ=60)
                                             CAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATG                                                  <  SRR4302545.1294506/94‑1 (MQ=60)
                                             CAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGGGGCGACGGGCATGGAGTAACTGGCGCAGCGTGGTGGAGTAAATG                                                  >  SRR4302545.2916995/1‑94 (MQ=60)
                                              AAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGC                                                 <  SRR4302545.4856406/94‑1 (MQ=60)
                                              AAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGC                                                 <  SRR4302545.10499336/94‑1 (MQ=60)
                                              AAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGC                                                 >  SRR4302545.10750114/1‑94 (MQ=60)
                                              AAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGC                                                 <  SRR4302545.10880281/94‑1 (MQ=60)
                                              AAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGC                                                 <  SRR4302545.4194798/94‑1 (MQ=60)
                                              AAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGGTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGC                                                 >  SRR4302545.10750115/1‑94 (MQ=60)
                                               AAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCC                                                >  SRR4302545.5021732/1‑94 (MQ=60)
                                                AAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCG                                               >  SRR4302545.8693027/1‑94 (MQ=60)
                                                AAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCG                                               >  SRR4302545.104359/1‑94 (MQ=60)
                                                AAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCG                                               >  SRR4302545.10464450/1‑94 (MQ=60)
                                                AAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCG                                               <  SRR4302545.3176571/94‑1 (MQ=60)
                                                AAACAGTTTTAGTGCTTCCGGAATATTAAACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGGAAATGCCG                                               >  SRR4302545.5346375/1‑94 (MQ=60)
                                                 AACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCGGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGGGGTGGAGTAAATGCCGG                                              >  SRR4302545.883592/1‑94 (MQ=60)
                                                 AACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGG                                              >  SRR4302545.6203112/1‑94 (MQ=60)
                                                 AACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGG                                              >  SRR4302545.5474682/1‑94 (MQ=60)
                                                 AACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGCGGAGTAAATGCCGG                                              >  SRR4302545.8330543/1‑94 (MQ=60)
                                                  ACCGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGT                                             <  SRR4302545.3056819/94‑1 (MQ=60)
                                                  ACAGTTTTCGTGCTTCCGGAATATTACACCGCGGATGTTGCAGGTCGCGACGGGCATGGCGTAACTCGCGCAGCGTGGTGGAGTAAATGCCGGT                                             >  SRR4302545.1782747/1‑94 (MQ=60)
                                                  ACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGT                                             <  SRR4302545.3056820/94‑1 (MQ=60)
                                                   catgTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTG                                            <  SRR4302545.8538547/90‑1 (MQ=60)
                                                   CAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTG                                            <  SRR4302545.9398954/94‑1 (MQ=60)
                                                   CAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTG                                            <  SRR4302545.9398955/94‑1 (MQ=60)
                                                   CAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTG                                            <  SRR4302545.4937852/94‑1 (MQ=60)
                                                   CAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGAGCAGCGGGGTGGAGTAAATGCCGGTG                                            <  SRR4302545.2929624/94‑1 (MQ=60)
                                                    AGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGA                                           <  SRR4302545.10087559/94‑1 (MQ=60)
                                                    AGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGA                                           <  SRR4302545.4602658/94‑1 (MQ=60)
                                                     GTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGAT                                          >  SRR4302545.6126211/1‑94 (MQ=60)
                                                     GTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGACGTAACTGGCGCATCCTGGGGGAGT‑AATGGCGGTGATC                                         >  SRR4302545.9136649/1‑94 (MQ=60)
                                                     GTTTTCGTGCTTCCGGAATATTAAACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGAT                                          <  SRR4302545.6009207/94‑1 (MQ=60)
                                                       TTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCG                                        <  SRR4302545.423885/94‑1 (MQ=60)
                                                       TTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCG                                        >  SRR4302545.5951562/1‑94 (MQ=60)
                                                       TTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCG                                        >  SRR4302545.6795991/1‑94 (MQ=60)
                                                       TTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCG                                        <  SRR4302545.8834255/94‑1 (MQ=60)
                                                         TCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCA                                      <  SRR4302545.7428823/94‑1 (MQ=60)
                                                         TCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCA                                      <  SRR4302545.1372627/94‑1 (MQ=60)
                                                          TGTGCTTACGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCAC                                     >  SRR4302545.10925813/1‑94 (MQ=60)
                                                          CGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCAC                                     <  SRR4302545.3387865/94‑1 (MQ=60)
                                                          CGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCAC                                     >  SRR4302545.5071576/1‑94 (MQ=60)
                                                           GTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGTCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACT                                    <  SRR4302545.6873516/94‑1 (MQ=60)
                                                           GTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACT                                    <  SRR4302545.2985034/94‑1 (MQ=60)
                                                           GTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACT                                    <  SRR4302545.531021/94‑1 (MQ=60)
                                                           GTGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACT                                    <  SRR4302545.8047157/94‑1 (MQ=60)
                                                            TGCTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTG                                   <  SRR4302545.2133687/94‑1 (MQ=60)
                                                              GTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGAAGTAAATGCCGGTGATCGCACTGTT                                 <  SRR4302545.1460221/94‑1 (MQ=60)
                                                              CTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTT                                 >  SRR4302545.9601315/1‑94 (MQ=60)
                                                              CTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTT                                 <  SRR4302545.9597845/94‑1 (MQ=60)
                                                              CTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTT                                 >  SRR4302545.7340925/1‑94 (MQ=60)
                                                              CTTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGAGGTGGAGTAAATGCCGGTGATCGCACTGTT                                 >  SRR4302545.7417475/1‑94 (MQ=60)
                                                               TTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTG                                >  SRR4302545.6263408/1‑94 (MQ=60)
                                                               TTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTG                                >  SRR4302545.6263407/1‑94 (MQ=60)
                                                               TTCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTG                                <  SRR4302545.10354626/94‑1 (MQ=60)
                                                                TCCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGT                               <  SRR4302545.5371902/94‑1 (MQ=60)
                                                                 CCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTG                              >  SRR4302545.84711/1‑94 (MQ=60)
                                                                 CCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTG                              <  SRR4302545.757687/94‑1 (MQ=60)
                                                                 CCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTG                              >  SRR4302545.9352136/1‑94 (MQ=60)
                                                                 CCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTG                              >  SRR4302545.10866133/1‑94 (MQ=60)
                                                                 CCGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTG                              >  SRR4302545.6989091/1‑94 (MQ=60)
                                                                  CGGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGCAAATGCCGGTGATCGCACTGTTGTGC                             >  SRR4302545.10386030/1‑94 (MQ=60)
                                                                   TGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCTGTGATCGCACTGTTGTGCA                            <  SRR4302545.8655428/94‑1 (MQ=60)
                                                                   GGAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCA                            <  SRR4302545.443894/94‑1 (MQ=60)
                                                                    GAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCGTGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCAC                           >  SRR4302545.7427642/1‑94 (MQ=60)
                                                                    GAATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCAC                           >  SRR4302545.2031250/1‑94 (MQ=60)
                                                                     AATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACC                          <  SRR4302545.9070083/94‑1 (MQ=60)
                                                                      ATATTACTCTGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGgtgtgtatca                         >  SRR4302545.9625284/1‑84 (MQ=60)
                                                                      ATATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCA                         <  SRR4302545.5026392/94‑1 (MQ=60)
                                                                       aatgACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCAT                        <  SRR4302545.6173493/90‑1 (MQ=60)
                                                                       TATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGCGTAAATGCCGGTGATCGCACTGTTGTGCCCCAT                        >  SRR4302545.8499921/1‑94 (MQ=60)
                                                                       TATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCAT                        <  SRR4302545.10558316/94‑1 (MQ=60)
                                                                        ATTACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATG                       >  SRR4302545.7869561/1‑94 (MQ=60)
                                                                          TACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGT                     <  SRR4302545.667929/94‑1 (MQ=60)
                                                                          TACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGT                     <  SRR4302545.3743499/94‑1 (MQ=60)
                                                                          TACACAGCGGTGGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGT                     <  SRR4302545.667928/94‑1 (MQ=60)
                                                                           ACACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTG                    <  SRR4302545.6837889/94‑1 (MQ=60)
                                                                            CACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGT                   <  SRR4302545.9794115/94‑1 (MQ=60)
                                                                            CACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGT                   <  SRR4302545.6641056/94‑1 (MQ=60)
                                                                            CACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGT                   >  SRR4302545.8932229/1‑94 (MQ=60)
                                                                            CACAGCGGTTGTTGCAGGGGGCGACGGGCATGGCGTAACTGTCGCAGCGTGGTGTAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGT                   <  SRR4302545.2416153/94‑1 (MQ=60)
                                                                             ACAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTA                  >  SRR4302545.3950591/1‑94 (MQ=60)
                                                                              CAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAG                 <  SRR4302545.1305869/94‑1 (MQ=60)
                                                                              CAGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAG                 <  SRR4302545.6014976/94‑1 (MQ=60)
                                                                               AGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGA                >  SRR4302545.7086294/1‑94 (MQ=60)
                                                                               AGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGA                <  SRR4302545.8521592/94‑1 (MQ=60)
                                                                               AGCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGA                <  SRR4302545.8521593/94‑1 (MQ=60)
                                                                                GCGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAA               >  SRR4302545.810049/1‑94 (MQ=60)
                                                                                 CGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAAC              >  SRR4302545.650387/1‑94 (MQ=60)
                                                                                 CGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAAC              <  SRR4302545.10204992/94‑1 (MQ=60)
                                                                                 CGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAAC              <  SRR4302545.3022350/94‑1 (MQ=60)
                                                                                 CGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAAC              >  SRR4302545.6288798/1‑94 (MQ=60)
                                                                                 CGGTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATAGCACTGTTGTGCACCATGGTGTAGAAC              >  SRR4302545.1854955/1‑94 (MQ=60)
                                                                                   GTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACAT            <  SRR4302545.1267174/94‑1 (MQ=60)
                                                                                   GTTGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACAT            >  SRR4302545.245320/1‑94 (MQ=60)
                                                                                   GTTGTTGCAGGCGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACAT            >  SRR4302545.1814362/1‑94 (MQ=60)
                                                                                     TGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATAC          <  SRR4302545.10484130/94‑1 (MQ=60)
                                                                                     TGTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATAC          >  SRR4302545.5630651/1‑94 (MQ=60)
                                                                                      GTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACG         >  SRR4302545.9596414/1‑94 (MQ=60)
                                                                                      GTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACG         <  SRR4302545.2990635/94‑1 (MQ=60)
                                                                                      GTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACG         <  SRR4302545.9240306/94‑1 (MQ=60)
                                                                                      GTTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACG         <  SRR4302545.9107689/94‑1 (MQ=60)
                                                                                      GTTGCAGGGGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGCTGTGCCCCATGGTGTAGAACATACG         >  SRR4302545.8392281/1‑94 (MQ=60)
                                                                                       TTGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGC        <  SRR4302545.5722001/94‑1 (MQ=60)
                                                                                        TGCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCA       >  SRR4302545.5137905/1‑94 (MQ=60)
                                                                                         GCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAA      <  SRR4302545.6011102/94‑1 (MQ=60)
                                                                                         GCAGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAA      >  SRR4302545.64352/1‑94 (MQ=60)
                                                                                           AGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGA    >  SRR4302545.5465159/1‑94 (MQ=60)
                                                                                           AGGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGA    >  SRR4302545.9807342/1‑94 (MQ=60)
                                                                                            GGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGAT   >  SRR4302545.1852374/1‑94 (MQ=60)
                                                                                            GGTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGAG   >  SRR4302545.9412727/1‑94 (MQ=60)
                                                                                             GTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATG  <  SRR4302545.9378031/94‑1 (MQ=60)
                                                                                             GTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATG  <  SRR4302545.1552872/94‑1 (MQ=60)
                                                                                             GTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATG  <  SRR4302545.4184596/94‑1 (MQ=60)
                                                                                             GTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATG  <  SRR4302545.10237712/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATG  >  NC_000913/187340‑187526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑