Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 207,737 C→T T871I (ACC→ATC)  dnaE → DNA polymerase III alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913207,7370CT98.6% 1404.4 / ‑0.4 369T871I (ACC→ATC) dnaEDNA polymerase III alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (4/1);  new base T (211/153);  total (215/154)
Fisher's exact test for biased strand distribution p-value = 4.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

Only 100 of 376 total aligned reads displayed.

GTCGGTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTC  >  NC_000913/207646‑207827
                                                                                           |                                                                                          
gTCGGTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCa                                                                                          <  2:4577715/94‑1 (MQ=255)
gTCGGTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCa                                                                                          <  2:7016582/94‑1 (MQ=255)
 tCGGTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCaa                                                                                         <  2:10272307/94‑1 (MQ=255)
   ggTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCaaaa                                                                                       >  1:2007896/1‑94 (MQ=255)
     tGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCTTACCAACACAAAAAAg                                                                                     >  2:1549554/1‑94 (MQ=255)
     tGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCAAAAAg                                                                                     <  2:7175986/94‑1 (MQ=255)
       aaGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGtt                                                                                   >  1:6274133/1‑94 (MQ=255)
         ggTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGa                                                                                 >  1:10011099/1‑94 (MQ=255)
           tCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAAc                                                                               <  1:2648405/94‑1 (MQ=255)
           tCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAAc                                                                               <  1:2453626/94‑1 (MQ=255)
            ccGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAAcg                                                                              >  1:8779097/1‑93 (MQ=255)
             cGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCg                                                                             <  2:3394811/94‑1 (MQ=255)
             cGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCg                                                                             >  2:2343342/1‑94 (MQ=255)
              gATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGt                                                                            >  1:6658528/1‑94 (MQ=255)
               aTTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTc                                                                           <  1:8941680/94‑1 (MQ=255)
               aTTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTc                                                                           <  2:2926581/94‑1 (MQ=255)
               aTTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTc                                                                           <  1:1708755/94‑1 (MQ=255)
                ttGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTcg                                                                          >  1:4510105/1‑94 (MQ=255)
                 tGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGCCATCAAAAAGTTGAACCGTcgc                                                                         >  1:8111142/1‑94 (MQ=255)
                  gAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTcgcg                                                                        <  2:1018487/94‑1 (MQ=255)
                   aGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGCTGAACCGTCGCGt                                                                       >  2:2226600/1‑94 (MQ=255)
                       catcatCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTg                                                                   <  1:4907640/94‑1 (MQ=255)
                       catcatCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTg                                                                   <  2:8812426/94‑1 (MQ=255)
                       catcatCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTg                                                                   <  2:1227930/94‑1 (MQ=255)
                       catcatCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTg                                                                   >  2:1834195/1‑94 (MQ=255)
                        atcatcGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTgg                                                                  <  2:6634923/94‑1 (MQ=255)
                        atcatcGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTgg                                                                  >  1:7302464/1‑94 (MQ=255)
                          catcGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGaa                                                                >  1:400114/1‑94 (MQ=255)
                          catcGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGaa                                                                >  1:8341533/1‑94 (MQ=255)
                           atcGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGaaa                                                               >  2:10490704/1‑94 (MQ=255)
                            tcGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGaaaa                                                              >  2:2773007/1‑94 (MQ=255)
                             cGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGaaaaa                                                             >  1:5105149/1‑94 (MQ=255)
                              gAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAAc                                                            >  1:10433138/1‑94 (MQ=255)
                              gAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAAc                                                            <  2:5142573/94‑1 (MQ=255)
                              gAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAAc                                                            <  2:1879250/94‑1 (MQ=255)
                              gAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAAc                                                            >  1:3851037/1‑94 (MQ=255)
                               aaGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACt                                                           >  1:9880507/1‑94 (MQ=255)
                               aaGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACt                                                           <  1:845726/94‑1 (MQ=255)
                                aGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTg                                                          >  2:8599700/1‑94 (MQ=255)
                                 gCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGa                                                         <  2:5506521/94‑1 (MQ=255)
                                  cccGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGAt                                                        <  2:10020159/94‑1 (MQ=255)
                                  cccGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGAt                                                        <  1:6907519/94‑1 (MQ=255)
                                    cGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCa                                                      >  1:5236451/1‑94 (MQ=255)
                                    cGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCa                                                      >  1:9268704/1‑94 (MQ=255)
                                      taataaAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATg                                                    >  1:5772800/1‑94 (MQ=255)
                                      taataaAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATg                                                    <  1:5423029/94‑1 (MQ=255)
                                      taataaAAGCGGCTACTACCGCGAACAATATGATCAATGCGCAAATACCAACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATg                                                    <  2:9386279/94‑1 (MQ=255)
                                       aataaAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGt                                                   <  2:8500685/94‑1 (MQ=255)
                                       aataaAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGt                                                   <  2:1653703/94‑1 (MQ=255)
                                           aaGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCgg                                               <  2:3586284/94‑1 (MQ=255)
                                           aaGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCgg                                               <  2:3294529/94‑1 (MQ=255)
                                              gcggcTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGc                                            >  1:4409172/1‑94 (MQ=255)
                                               cggcTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAATTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCg                                           >  1:4144877/1‑94 (MQ=255)
                                                ggcTACTTCCGCGAACTGTTTGATCTCTGCGCCAGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGt                                          >  1:3337716/1‑94 (MQ=255)
                                                 gcTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGtt                                         >  1:3705924/1‑94 (MQ=255)
                                                  cTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGttt                                        >  2:9830314/1‑94 (MQ=255)
                                                  cTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGttt                                        >  2:9660397/1‑94 (MQ=255)
                                                  cTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGttt                                        <  1:3611771/94‑1 (MQ=255)
                                                    aCTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGa                                      >  1:600552/1‑94 (MQ=255)
                                                     cTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGAc                                     >  2:5027440/1‑94 (MQ=255)
                                                      ttCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGAcc                                    <  2:3878934/94‑1 (MQ=255)
                                                      ttCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGAcc                                    <  2:7760312/94‑1 (MQ=255)
                                                       tCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCg                                   >  2:8078868/1‑94 (MQ=255)
                                                         cgcgAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTc                                 >  1:10604900/1‑94 (MQ=255)
                                                          gcgAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCt                                >  2:2727927/1‑94 (MQ=255)
                                                            gAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTg                              >  2:1989080/1‑94 (MQ=255)
                                                            gAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTg                              >  2:6128889/1‑94 (MQ=255)
                                                              aCTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTggg                            <  2:4305903/94‑1 (MQ=255)
                                                              aCTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTggg                            <  2:4784733/94‑1 (MQ=255)
                                                                 gTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCca                         >  2:7365870/1‑94 (MQ=255)
                                                                   ttGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCcaca                       <  1:2819434/94‑1 (MQ=255)
                                                                   ttGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCcaca                       >  1:6723169/1‑94 (MQ=255)
                                                                    tGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGTCACAt                      >  1:10263798/1‑94 (MQ=255)
                                                                     gATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATc                     <  1:1106952/94‑1 (MQ=255)
                                                                     gATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATc                     >  1:6417591/1‑94 (MQ=255)
                                                                        ctctGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATcgcg                  >  1:4842017/1‑94 (MQ=255)
                                                                        ctctGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATcgcg                  >  1:9684110/1‑94 (MQ=255)
                                                                         tctGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCgcgc                 <  1:9437378/94‑1 (MQ=255)
                                                                          ctGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCa                <  1:7972072/94‑1 (MQ=255)
                                                                          ctGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCa                >  2:6429445/1‑94 (MQ=255)
                                                                           tGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAg               >  2:8956354/1‑94 (MQ=255)
                                                                            gcgcCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAgc              >  1:1016343/1‑94 (MQ=255)
                                                                             cgcCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAACAACTGATCATGTCCGCGGCGTTTGACCGTCTTGGGCCACATCGGGCAggc             >  1:3218690/1‑92 (MQ=255)
                                                                              gcCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAgcgc            <  2:3127414/94‑1 (MQ=255)
                                                                              gcCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAgcgc            <  1:2324576/94‑1 (MQ=255)
                                                                              gcCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAgcgc            >  1:7419392/1‑94 (MQ=255)
                                                                                ccGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCtg          >  2:6488075/1‑94 (MQ=255)
                                                                                ccGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCtg          >  1:1592657/1‑94 (MQ=255)
                                                                                   tACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCtgatg       <  1:1247585/94‑1 (MQ=255)
                                                                                   tACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCtgatg       >  2:6945731/1‑94 (MQ=255)
                                                                                   tACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCtgatg       <  2:7997910/94‑1 (MQ=255)
                                                                                    aCCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCtgatga      <  1:7646832/94‑1 (MQ=255)
                                                                                    aCCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCtgatga      >  1:3789226/1‑94 (MQ=255)
                                                                                       gACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACt   >  2:1384878/1‑94 (MQ=255)
                                                                                       gACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACt   >  1:5662760/1‑94 (MQ=255)
                                                                                       gACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACt   >  2:6233936/1‑94 (MQ=255)
                                                                                       gACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACt   <  2:58764/94‑1 (MQ=255)
                                                                                       gACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGCTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACt   >  1:4293498/1‑94 (MQ=255)
                                                                                        acatCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTc  <  1:1439503/94‑1 (MQ=255)
                                                                                        acatCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTc  <  1:10513272/94‑1 (MQ=255)
                                                                                           |                                                                                          
GTCGGTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTC  >  NC_000913/207646‑207827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 220 total aligned reads displayed.
GGGTCGGTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCT  >  NC_000913/207644‑207830
                                                                                             |                                                                                             
GGGTCGGTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACAT                                                                                               <  SRR4302545.5848104/94‑1 (MQ=60)
GGGTCGGTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACAT                                                                                               <  SRR4302545.10476731/94‑1 (MQ=60)
GGGTCGGTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACAT                                                                                               <  SRR4302545.10441384/94‑1 (MQ=60)
 GGTCGGTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATC                                                                                              <  SRR4302545.1184863/94‑1 (MQ=60)
  GTCGGTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCA                                                                                             <  SRR4302545.3693392/94‑1 (MQ=60)
   TCGGTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAA                                                                                            >  SRR4302545.9500279/1‑94 (MQ=60)
     GGTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAA                                                                                          >  SRR4302545.6065040/1‑94 (MQ=60)
     GGTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAA                                                                                          >  SRR4302545.12702/1‑94 (MQ=60)
      GTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAA                                                                                         <  SRR4302545.10800230/94‑1 (MQ=60)
        TAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGT                                                                                       <  SRR4302545.7033050/94‑1 (MQ=60)
        GAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGT                                                                                       >  SRR4302545.2382083/1‑94 (MQ=60)
         AAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCTAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTT                                                                                      <  SRR4302545.107363/94‑1 (MQ=60)
         AAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTT                                                                                      >  SRR4302545.6445198/1‑94 (MQ=60)
          AGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTG                                                                                     >  SRR4302545.404554/1‑94 (MQ=60)
           GGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGA                                                                                    >  SRR4302545.10307860/1‑94 (MQ=60)
            GTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAA                                                                                   >  SRR4302545.2545470/1‑94 (MQ=60)
             TCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAAC                                                                                  >  SRR4302545.4451200/1‑94 (MQ=60)
             TCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAAC                                                                                  >  SRR4302545.8811233/1‑94 (MQ=60)
             TCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAAC                                                                                  <  SRR4302545.2712171/94‑1 (MQ=60)
             TCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAAC                                                                                  <  SRR4302545.2513060/94‑1 (MQ=60)
             TCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAAC                                                                                  <  SRR4302545.679046/94‑1 (MQ=60)
              CCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACG                                                                                 >  SRR4302545.9033882/1‑94 (MQ=60)
              CCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACC                                                                                 <  SRR4302545.7881743/94‑1 (MQ=60)
              CCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACC                                                                                 <  SRR4302545.9892320/94‑1 (MQ=60)
               CGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCG                                                                                >  SRR4302545.2090777/1‑94 (MQ=60)
               CGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCG                                                                                <  SRR4302545.6680726/94‑1 (MQ=60)
                GATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGT                                                                               <  SRR4302545.7761029/94‑1 (MQ=60)
                 ATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCCAAAAGTTGAACCGTC                                                                              >  SRR4302545.2554778/1‑94 (MQ=60)
                 ATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTC                                                                              <  SRR4302545.1749410/94‑1 (MQ=60)
                 ATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTC                                                                              <  SRR4302545.1749406/94‑1 (MQ=60)
                 ATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTC                                                                              <  SRR4302545.9201751/94‑1 (MQ=60)
                 ATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTC                                                                              >  SRR4302545.6857664/1‑94 (MQ=60)
                  TTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCG                                                                             >  SRR4302545.4625538/1‑94 (MQ=60)
                  TTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCC                                                                             >  SRR4302545.4625537/1‑94 (MQ=60)
                   TGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGCCATCAAAAAGTTGAACCGTCGC                                                                            >  SRR4302545.8345124/1‑94 (MQ=60)
                   TGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGC                                                                            >  SRR4302545.6448063/1‑94 (MQ=60)
                   TGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGC                                                                            >  SRR4302545.8793966/1‑94 (MQ=60)
                    GAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCG                                                                           <  SRR4302545.6951188/94‑1 (MQ=60)
                    GAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCG                                                                           >  SRR4302545.7917013/1‑94 (MQ=60)
                     AGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGT                                                                          <  SRR4302545.1056742/94‑1 (MQ=60)
                     AGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGT                                                                          >  SRR4302545.8269084/1‑94 (MQ=60)
                      GGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTG                                                                         <  SRR4302545.4423827/94‑1 (MQ=60)
                       GCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGC                                                                        <  SRR4302545.3070979/94‑1 (MQ=60)
                       GCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGC                                                                        <  SRR4302545.10759407/94‑1 (MQ=60)
                        CCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCT                                                                       >  SRR4302545.7409601/1‑94 (MQ=60)
                         CATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTG                                                                      <  SRR4302545.5032197/94‑1 (MQ=60)
                         CATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTG                                                                      >  SRR4302545.1475595/1‑94 (MQ=60)
                          CTCATCGAAGCCCGTAATAAAGGCTGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTCCCGACATCAAAAAGTTGAACCGTCGCGTGCTGG                                                                     <  SRR4302545.2897107/94‑1 (MQ=60)
                          ATCATCGAAGCCCGTAATAAAGGCGGCTACTTCGGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGG                                                                     <  SRR4302545.8648715/94‑1 (MQ=60)
                          ATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGG                                                                     >  SRR4302545.7507242/1‑94 (MQ=60)
                          ATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGG                                                                     <  SRR4302545.8709443/94‑1 (MQ=60)
                          ATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGG                                                                     >  SRR4302545.6719922/1‑94 (MQ=60)
                           TCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGA                                                                    <  SRR4302545.3528186/94‑1 (MQ=60)
                            CATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAA                                                                   >  SRR4302545.7706717/1‑94 (MQ=60)
                            CATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAA                                                                   >  SRR4302545.5764858/1‑94 (MQ=60)
                            CATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAA                                                                   >  SRR4302545.409021/1‑94 (MQ=60)
                            CATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAA                                                                   >  SRR4302545.8583217/1‑94 (MQ=60)
                            CATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCAAAAAGTTATACCGTCGCGTGCTGGAA                                                                   >  SRR4302545.10379338/1‑94 (MQ=60)
                               CGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAA                                                                >  SRR4302545.4174050/1‑94 (MQ=60)
                               CGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAA                                                                >  SRR4302545.72502/1‑94 (MQ=60)
                               CGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAA                                                                >  SRR4302545.5235615/1‑94 (MQ=60)
                                GAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAAC                                                               >  SRR4302545.3947167/1‑94 (MQ=60)
                                GAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAAC                                                               <  SRR4302545.8266001/94‑1 (MQ=60)
                                 AAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACT                                                              >  SRR4302545.10174106/1‑94 (MQ=60)
                                 AAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACT                                                              >  SRR4302545.9577187/1‑94 (MQ=60)
                                 AAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACT                                                              <  SRR4302545.864692/94‑1 (MQ=60)
                                  AGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTG                                                             <  SRR4302545.1314382/94‑1 (MQ=60)
                                  AGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTG                                                             >  SRR4302545.5843248/1‑94 (MQ=60)
                                   GCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGA                                                            <  SRR4302545.5541497/94‑1 (MQ=60)
                                    CCCGTAATAAAGGCGGCTCCTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCATGCTGGAAAAACTGAT                                                           <  SRR4302545.681592/94‑1 (MQ=60)
                                    CCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGAT                                                           >  SRR4302545.1186275/1‑94 (MQ=60)
                                    CCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGAT                                                           <  SRR4302545.10874317/94‑1 (MQ=60)
                                    CCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGAT                                                           <  SRR4302545.7097832/94‑1 (MQ=60)
                                    CCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGAT                                                           <  SRR4302545.1999947/94‑1 (MQ=60)
                                     CCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATC                                                          <  SRR4302545.9566961/94‑1 (MQ=60)
                                      CGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCA                                                         <  SRR4302545.5366966/94‑1 (MQ=60)
                                      CGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCA                                                         >  SRR4302545.5371531/1‑94 (MQ=60)
                                      CGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCA                                                         >  SRR4302545.5464176/1‑94 (MQ=60)
                                      CGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCA                                                         <  SRR4302545.10539265/94‑1 (MQ=60)
                                      CGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCA                                                         >  SRR4302545.9540419/1‑94 (MQ=60)
                                       GTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCAT                                                        <  SRR4302545.10020912/94‑1 (MQ=60)
                                       GTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCAT                                                        >  SRR4302545.6118351/1‑94 (MQ=60)
                                       GTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGGTGAACCGTCGCGTTCTGGAAAAATTGATCAG                                                        >  SRR4302545.8320090/1‑94 (MQ=60)
                                        TAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATG                                                       <  SRR4302545.3842445/94‑1 (MQ=60)
                                        TAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATG                                                       <  SRR4302545.5564765/94‑1 (MQ=60)
                                        TAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATG                                                       >  SRR4302545.5927946/1‑94 (MQ=60)
                                         AATAAAGGCGGCTCCTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGT                                                      <  SRR4302545.4911275/94‑1 (MQ=60)
                                         AATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGT                                                      <  SRR4302545.4249435/94‑1 (MQ=60)
                                         AATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGT                                                      <  SRR4302545.9521201/94‑1 (MQ=60)
                                         AATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGT                                                      >  SRR4302545.405699/1‑94 (MQ=60)
                                         AATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGT                                                      <  SRR4302545.108795/94‑1 (MQ=60)
                                          ATAAAGGCGGCTACTTCCGCGAAGTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTC                                                     <  SRR4302545.7465071/94‑1 (MQ=60)
                                          ATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTC                                                     >  SRR4302545.1050754/1‑94 (MQ=60)
                                           TAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCC                                                    <  SRR4302545.910073/94‑1 (MQ=60)
                                            AAAGGCGGTTACTTCCGCGCACTGTTTGATCTCTGCGCCCGTACCGACATCTAAAAGTTGAACCTTGGCGCGCTGGAAAAAGTCATCATTTCCG                                                   >  SRR4302545.2819270/1‑94 (MQ=46)
                                            AAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCG                                                   >  SRR4302545.2819271/1‑94 (MQ=60)
                                            AAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCG                                                   <  SRR4302545.10631682/94‑1 (MQ=60)
                                              AGGGGGCTACTTCCGCGAACTGTTTTATCTCTGCGCCCATACCGACATCAAGAAGTTGAACCGTCGCGTGCTGTAAAAAGGGATCATGTCCTGG                                                 >  SRR4302545.3842058/1‑94 (MQ=60)
                                              AGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGG                                                 <  SRR4302545.9132288/94‑1 (MQ=60)
                                              AGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGG                                                 >  SRR4302545.3842060/1‑94 (MQ=60)
                                                GCGGCTACTTCCGCGAATTGTTTGATCTCTGCGTCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGTC                                               <  SRR4302545.5810773/94‑1 (MQ=60)
                                                GCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGC                                               >  SRR4302545.4521137/1‑94 (MQ=60)
                                                 CGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAATTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCG                                              >  SRR4302545.4248423/1‑94 (MQ=60)
                                                 CGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCG                                              >  SRR4302545.2615667/1‑94 (MQ=60)
                                                 CGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCG                                              <  SRR4302545.4190943/94‑1 (MQ=60)
                                                  GGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGT                                             >  SRR4302545.4992688/1‑94 (MQ=60)
                                                  GGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGT                                             >  SRR4302545.4992687/1‑94 (MQ=60)
                                                  GGCTACTTCCGCGAACTGTTTGATCTCTGCGCCAGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGT                                             >  SRR4302545.3419281/1‑94 (MQ=60)
                                                   GGTACTGCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTT                                            <  SRR4302545.9036154/94‑1 (MQ=60)
                                                   GCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTT                                            >  SRR4302545.3796823/1‑94 (MQ=60)
                                                   GCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGGT                                            >  SRR4302545.3796824/1‑94 (MQ=60)
                                                    CTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTT                                           >  SRR4302545.5881672/1‑94 (MQ=60)
                                                    CTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTT                                           <  SRR4302545.3699809/94‑1 (MQ=60)
                                                    CTACTGCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTT                                           <  SRR4302545.5154336/94‑1 (MQ=60)
                                                     ttccTCCGCGTACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTG                                          >  SRR4302545.9668695/5‑94 (MQ=60)
                                                     TACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTG                                          <  SRR4302545.2858094/94‑1 (MQ=60)
                                                      ACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGA                                         >  SRR4302545.614086/1‑94 (MQ=60)
                                                       CTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGAC                                        >  SRR4302545.6312604/1‑94 (MQ=60)
                                                       CTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGAC                                        >  SRR4302545.840563/1‑94 (MQ=60)
                                                        TTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACC                                       >  SRR4302545.1636528/1‑94 (MQ=60)
                                                          CCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGT                                     <  SRR4302545.10903842/94‑1 (MQ=60)
                                                           CGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTC                                    >  SRR4302545.10917149/1‑94 (MQ=60)
                                                           CGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTC                                    >  SRR4302545.3133288/1‑94 (MQ=60)
                                                           CGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTC                                    >  SRR4302545.6457072/1‑94 (MQ=60)
                                                           CGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTC                                    >  SRR4302545.6457076/1‑94 (MQ=60)
                                                            GCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCT                                   >  SRR4302545.3389865/1‑94 (MQ=60)
                                                            GCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCT                                   >  SRR4302545.8577395/1‑94 (MQ=60)
                                                             CGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTT                                  >  SRR4302545.1086110/1‑94 (MQ=60)
                                                              GAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTG                                 <  SRR4302545.4399644/94‑1 (MQ=60)
                                                              GAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTG                                 >  SRR4302545.9774895/1‑94 (MQ=60)
                                                              GAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTG                                 >  SRR4302545.10513626/1‑94 (MQ=60)
                                                              GAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTG                                 >  SRR4302545.4584829/1‑94 (MQ=60)
                                                               AACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGG                                <  SRR4302545.1018697/94‑1 (MQ=60)
                                                                ACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGG                               >  SRR4302545.5043463/1‑94 (MQ=60)
                                                                ACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGG                               >  SRR4302545.181918/1‑94 (MQ=60)
                                                                 CTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGC                              >  SRR4302545.63590/1‑94 (MQ=60)
                                                                  TGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCC                             >  SRR4302545.10520570/1‑94 (MQ=60)
                                                                  TGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCC                             <  SRR4302545.7591119/94‑1 (MQ=60)
                                                                   GTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCA                            <  SRR4302545.5687279/94‑1 (MQ=60)
                                                                    TTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCAC                           <  SRR4302545.1036955/94‑1 (MQ=60)
                                                                     TTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACA                          <  SRR4302545.2887681/94‑1 (MQ=60)
                                                                     TTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACA                          <  SRR4302545.1441295/94‑1 (MQ=60)
                                                                     TTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACA                          >  SRR4302545.6907487/1‑94 (MQ=60)
                                                                      TGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGTCACAT                         >  SRR4302545.10566653/1‑94 (MQ=60)
                                                                       GATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATC                        >  SRR4302545.6593059/1‑94 (MQ=60)
                                                                       GATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATC                        >  SRR4302545.6912304/1‑94 (MQ=60)
                                                                       GATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATC                        <  SRR4302545.1131061/94‑1 (MQ=60)
                                                                        ATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCG                       >  SRR4302545.8429941/1‑94 (MQ=60)
                                                                        ATCGCGGCGCCCGTCCCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGCCCGTCTTGGGCCACATCG                       <  SRR4302545.3982123/94‑1 (MQ=60)
                                                                          CTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCG                     >  SRR4302545.4965455/1‑94 (MQ=60)
                                                                           TCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGC                    <  SRR4302545.9714658/94‑1 (MQ=60)
                                                                            CTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCA                   >  SRR4302545.2494178/1‑94 (MQ=60)
                                                                            CTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCA                   <  SRR4302545.8198714/94‑1 (MQ=60)
                                                                            CTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCA                   >  SRR4302545.7540431/1‑94 (MQ=60)
                                                                            CTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCA                   >  SRR4302545.3771338/1‑94 (MQ=60)
                                                                             GGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGTAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTTGGCCACATCGCGCAG                  <  SRR4302545.1871286/94‑1 (MQ=60)
                                                                              GCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGC                 >  SRR4302545.1038755/1‑94 (MQ=60)
                                                                               CGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCG                <  SRR4302545.1302763/94‑1 (MQ=60)
                                                                               CGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCG                <  SRR4302545.5828388/94‑1 (MQ=60)
                                                                                GCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGC               >  SRR4302545.4985506/1‑94 (MQ=60)
                                                                                GCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGC               >  SRR4302545.4405573/1‑94 (MQ=60)
                                                                                GCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGC               <  SRR4302545.2381176/94‑1 (MQ=60)
                                                                                GCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGC               >  SRR4302545.7629135/1‑94 (MQ=60)
                                                                                GCCCGCACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGCCCGTCTTGGTCCACATCGCGCCGCGC               >  SRR4302545.3010240/1‑94 (MQ=60)
                                                                                 CCCGTACCGACATCGAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGGGCAGCGCT              >  SRR4302545.5110872/1‑94 (MQ=60)
                                                                                 CCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCT              <  SRR4302545.1117976/94‑1 (MQ=60)
                                                                                 CCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCT              <  SRR4302545.2344058/94‑1 (MQ=60)
                                                                                  CCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTG             >  SRR4302545.2807362/1‑94 (MQ=60)
                                                                                  CCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTG             >  SRR4302545.2238382/1‑94 (MQ=60)
                                                                                  CCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTG             >  SRR4302545.1630343/1‑94 (MQ=60)
                                                                                   CGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGA            >  SRR4302545.3707826/1‑94 (MQ=60)
                                                                                   CGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGA            >  SRR4302545.4722743/1‑94 (MQ=60)
                                                                                    GTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGAT           <  SRR4302545.3944381/94‑1 (MQ=60)
                                                                                    GTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGAT           >  SRR4302545.4248090/1‑94 (MQ=60)
                                                                                    GTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGAT           >  SRR4302545.3308754/1‑94 (MQ=60)
                                                                                    GTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGAT           >  SRR4302545.1668277/1‑94 (MQ=60)
                                                                                     TACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATG          >  SRR4302545.367553/1‑94 (MQ=60)
                                                                                     TACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATG          <  SRR4302545.1275623/94‑1 (MQ=60)
                                                                                      ACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGA         <  SRR4302545.10029878/94‑1 (MQ=60)
                                                                                      ACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGA         <  SRR4302545.10029879/94‑1 (MQ=60)
                                                                                      ACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGA         >  SRR4302545.3883074/1‑94 (MQ=60)
                                                                                      ACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGA         <  SRR4302545.7863226/94‑1 (MQ=60)
                                                                                      ACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGAGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGA         >  SRR4302545.3883075/1‑94 (MQ=60)
                                                                                         GACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACT      >  SRR4302545.148767/1‑94 (MQ=60)
                                                                                         GACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACT      >  SRR4302545.5602592/1‑94 (MQ=60)
                                                                                         GACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACT      <  SRR4302545.5438815/94‑1 (MQ=60)
                                                                                         GACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGACACAATGCGCAGCGCTGATGAACT      >  SRR4302545.9780027/1‑94 (MQ=60)
                                                                                         GACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGCTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACT      >  SRR4302545.4401520/1‑94 (MQ=60)
                                                                                          ACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTC     <  SRR4302545.1472251/94‑1 (MQ=60)
                                                                                          ACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTC     <  SRR4302545.10823085/94‑1 (MQ=60)
                                                                                           CATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGATCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCG    >  SRR4302545.1671388/1‑94 (MQ=60)
                                                                                           CATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCG    <  SRR4302545.8280412/94‑1 (MQ=60)
                                                                                           CATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCG    >  SRR4302545.1542110/1‑94 (MQ=60)
                                                                                           CATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCG    <  SRR4302545.6617351/94‑1 (MQ=60)
                                                                                           CATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCG    >  SRR4302545.7606818/1‑94 (MQ=60)
                                                                                           CATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCG    >  SRR4302545.7604914/1‑94 (MQ=60)
                                                                                            ATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGC   >  SRR4302545.421451/1‑94 (MQ=60)
                                                                                            ATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGC   >  SRR4302545.3617453/1‑94 (MQ=60)
                                                                                             TCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCT  <  SRR4302545.10222493/94‑1 (MQ=60)
                                                                                             TCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCT  >  SRR4302545.4082001/1‑94 (MQ=60)
                                                                                             |                                                                                             
GGGTCGGTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCT  >  NC_000913/207644‑207830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑