Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 207,737 C→T T871I (ACC→ATC)  dnaE → DNA polymerase III alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913207,7370CT100.0% 725.0 / NA 202T871I (ACC→ATC) dnaEDNA polymerase III alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (109/93);  total (109/93)

Only 100 of 204 total aligned reads displayed.

TCGGTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAAC  >  NC_000913/207647‑207825
                                                                                          |                                                                                        
     gAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAg                                                                                   >  1:2448810/1‑93 (MQ=255)
      aaGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGt                                                                                  >  2:4694563/1‑93 (MQ=255)
       aGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGtt                                                                                 <  2:381757/93‑1 (MQ=255)
        ggTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTg                                                                                >  2:4888632/1‑93 (MQ=255)
        ggTCCGATTGAGCCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTg                                                                                <  2:5397122/93‑1 (MQ=255)
         gTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGa                                                                               <  2:359730/93‑1 (MQ=255)
         gTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGa                                                                               >  2:3623019/1‑93 (MQ=255)
          tCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGaa                                                                              <  2:564752/93‑1 (MQ=255)
           ccGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAAc                                                                             <  1:1218701/93‑1 (MQ=255)
           ccGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAAc                                                                             <  1:2206859/93‑1 (MQ=255)
            cGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGGTGAAcc                                                                            >  2:5247572/1‑93 (MQ=255)
             gATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCg                                                                           >  1:4033693/1‑93 (MQ=255)
             gATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCg                                                                           >  1:5425716/1‑93 (MQ=255)
               ttGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTc                                                                         >  1:4409335/1‑93 (MQ=255)
               ttGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTc                                                                         <  2:5261862/93‑1 (MQ=255)
               ttGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTc                                                                         >  1:1243539/1‑93 (MQ=255)
               ttGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATATCTGCGCCCGTACCGACATCCAAAAGTTGAACCGTc                                                                         >  1:2750930/1‑93 (MQ=255)
                tGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTcg                                                                        <  2:3750559/93‑1 (MQ=255)
                 gAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCAAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTcgc                                                                       >  1:4550194/1‑93 (MQ=255)
                  aGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTcgcg                                                                      >  1:240632/1‑93 (MQ=255)
                  aGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTcgcg                                                                      >  1:3333129/1‑93 (MQ=255)
                   ggCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGt                                                                     <  2:4464434/93‑1 (MQ=255)
                     ccATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGc                                                                   <  2:3915442/93‑1 (MQ=255)
                        tcatcGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTgg                                                                >  1:1641971/1‑93 (MQ=255)
                         catcGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGa                                                               >  2:2243517/1‑93 (MQ=255)
                              aaGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGGTGAACCGTCGCGTGCTGGAAAAAc                                                          <  2:1363954/93‑1 (MQ=255)
                               aGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACt                                                         <  1:4929494/93‑1 (MQ=255)
                               aGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACt                                                         >  1:3011836/1‑93 (MQ=255)
                                gCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTg                                                        <  1:6090302/93‑1 (MQ=255)
                                 cccGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGa                                                       >  1:2652243/1‑93 (MQ=255)
                                 cccGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGa                                                       <  2:5681990/93‑1 (MQ=255)
                                 cccGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGa                                                       <  1:311126/93‑1 (MQ=255)
                                  ccGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGAt                                                      <  2:3768766/93‑1 (MQ=255)
                                   cGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATGTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATc                                                     <  1:2882229/93‑1 (MQ=255)
                                   cGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATc                                                     >  2:703532/1‑93 (MQ=255)
                                   cGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATc                                                     <  1:1946231/93‑1 (MQ=255)
                                    gTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCa                                                    >  2:6411224/1‑93 (MQ=255)
                                    ctaataaAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCa                                                    <  2:2945370/92‑1 (MQ=255)
                                     taataaAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCAt                                                   >  1:278283/1‑93 (MQ=255)
                                      aataaAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATg                                                  >  2:6471370/1‑93 (MQ=255)
                                      aataaAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATg                                                  >  1:1145103/1‑93 (MQ=255)
                                       ataaAGGCGGCTACTTCCGCGCACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGAGTGCTGGAAAAACTGATCATGt                                                 >  2:1440372/1‑93 (MQ=255)
                                        taaAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTc                                                >  1:5094364/1‑93 (MQ=255)
                                        taaAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTc                                                >  1:272034/1‑93 (MQ=255)
                                         aaaGGCTGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTcc                                               >  2:1940412/1‑93 (MQ=255)
                                          aaGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCg                                              >  1:5696527/1‑93 (MQ=255)
                                            ggcggcTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCggg                                            <  1:2085580/93‑1 (MQ=255)
                                            ggcggcTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCggg                                            <  2:6110755/93‑1 (MQ=255)
                                               ggcTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCg                                         >  1:5362828/1‑93 (MQ=255)
                                                  tACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGttt                                      <  2:4326668/93‑1 (MQ=255)
                                                  tACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGttt                                      >  1:2045543/1‑93 (MQ=255)
                                                    cTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGa                                    >  1:3774282/1‑93 (MQ=255)
                                                    cTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGGCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGGTTGa                                    >  2:4531765/1‑93 (MQ=255)
                                                      tCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGAcc                                  >  1:855794/1‑93 (MQ=255)
                                                       ccGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCg                                 >  2:4243297/1‑93 (MQ=255)
                                                        cgcgAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGt                                >  1:3510306/1‑93 (MQ=255)
                                                        cgcgAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGt                                >  2:2347493/1‑93 (MQ=255)
                                                        cgcgAACGGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGGTTGCCCgc                                >  1:5625951/1‑92 (MQ=255)
                                                         gcgAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTc                               <  1:3711638/93‑1 (MQ=255)
                                                         gcgAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTc                               <  2:5054609/93‑1 (MQ=255)
                                                         gcgAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTc                               >  2:1697203/1‑93 (MQ=255)
                                                         gcgAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTc                               >  1:2818268/1‑93 (MQ=255)
                                                           gAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCtt                             <  1:3684838/93‑1 (MQ=255)
                                                           gAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCtt                             <  1:1418248/93‑1 (MQ=255)
                                                           gAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCtt                             <  2:2837944/93‑1 (MQ=255)
                                                           caaCTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGGCCGTCtt                             <  2:1823890/92‑1 (MQ=255)
                                                            aaCTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTg                            >  1:2981093/1‑93 (MQ=255)
                                                             aCTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTgg                           <  2:5021233/93‑1 (MQ=255)
                                                              cTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTtgg                          >  1:1476944/1‑93 (MQ=255)
                                                               tGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCCTGTCCGGGGCGTTTGACCGTCTTGGGc                         >  2:2070084/1‑93 (MQ=255)
                                                               tGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGc                         <  1:184724/93‑1 (MQ=255)
                                                               tGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGATGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGAGc                         >  2:3130568/1‑93 (MQ=255)
                                                                gTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTCGACCGTCTTGGGcc                        <  2:5924248/93‑1 (MQ=255)
                                                                 tttGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCca                       >  1:5349095/1‑93 (MQ=255)
                                                                 tttGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCca                       >  1:2595869/1‑93 (MQ=255)
                                                                  ttGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCcac                      >  2:1311813/1‑93 (MQ=255)
                                                                  ttGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCcac                      >  1:1812718/1‑93 (MQ=255)
                                                                   tGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCcaca                     >  1:5466465/1‑93 (MQ=255)
                                                                    gATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACAt                    <  2:3240996/93‑1 (MQ=255)
                                                                    gATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACAt                    >  1:6312498/1‑93 (MQ=255)
                                                                    gATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACAGTCGCGTGCTGGAAAAACTCATTATGTCTGGGGCGTTTGACCGTCTCTGGCCACAt                    >  2:2695655/1‑93 (MQ=255)
                                                                     aTCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATc                   <  1:4785776/93‑1 (MQ=255)
                                                                     aTCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATc                   <  2:4820710/93‑1 (MQ=255)
                                                                      tctcTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATcg                  >  1:2539843/1‑93 (MQ=255)
                                                                       ctctGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATcgc                 <  2:2977709/93‑1 (MQ=255)
                                                                        tctGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATcgcg                >  2:4335141/1‑93 (MQ=255)
                                                                          tGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCa              <  2:1200633/93‑1 (MQ=255)
                                                                           gcgcCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAg             >  1:4809528/1‑93 (MQ=255)
                                                                              cccGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAgcgc          <  1:6182491/93‑1 (MQ=255)
                                                                              cccGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAgcgc          <  1:5889188/93‑1 (MQ=255)
                                                                              cccGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAgcgc          <  1:3286751/93‑1 (MQ=255)
                                                                              cccGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAgcgc          <  1:6153667/93‑1 (MQ=255)
                                                                               ccGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGCCTTGGGCCACATCGCGCAGCGCt         <  1:421138/93‑1 (MQ=255)
                                                                                 gTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCtga       <  1:5626948/93‑1 (MQ=255)
                                                                                  tACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCtgat      >  1:19843/1‑93 (MQ=255)
                                                                                  tACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCtgat      >  1:4748282/1‑93 (MQ=255)
                                                                                    ccGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCtgatga    >  1:4751681/1‑93 (MQ=255)
                                                                                     cGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGaa   >  2:2163439/1‑93 (MQ=255)
                                                                                      gACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAAc  >  2:2169930/1‑93 (MQ=255)
                                                                                      gACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAAc  <  2:6222900/93‑1 (MQ=255)
                                                                                          |                                                                                        
TCGGTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAAC  >  NC_000913/207647‑207825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTCGGTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCT  >  NC_000913/207645‑207830
                                                                                            |                                                                                             
GGTCGGTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATC                                                                                              <  SRR4302537.3174409/94‑1 (MQ=60)
 GTCGGTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCA                                                                                             <  SRR4302537.5388837/94‑1 (MQ=60)
     GTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAA                                                                                         >  SRR4302537.2656649/1‑94 (MQ=60)
     GTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAA                                                                                         <  SRR4302537.483037/94‑1 (MQ=60)
      TGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCTCGTACCGACATCAAAAAG                                                                                        <  SRR4302537.326306/94‑1 (MQ=60)
      TGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAG                                                                                        >  SRR4302537.2466038/1‑94 (MQ=60)
        AAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTT                                                                                      >  SRR4302537.3887989/1‑94 (MQ=60)
        AAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTT                                                                                      >  SRR4302537.5388931/1‑94 (MQ=60)
         AGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTG                                                                                     <  SRR4302537.2492906/94‑1 (MQ=60)
         AGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTG                                                                                     <  SRR4302537.2998362/94‑1 (MQ=60)
         AGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGAGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTA                                                                                     >  SRR4302537.2817098/1‑94 (MQ=60)
          GGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGA                                                                                    <  SRR4302537.3002558/94‑1 (MQ=60)
           GTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAA                                                                                   <  SRR4302537.1560331/94‑1 (MQ=60)
            TCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGCAC                                                                                  >  SRR4302537.1270803/1‑94 (MQ=60)
             CCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACC                                                                                 <  SRR4302537.1227177/94‑1 (MQ=60)
             CCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACC                                                                                 <  SRR4302537.2222418/94‑1 (MQ=60)
              CGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCG                                                                                >  SRR4302537.4061925/1‑94 (MQ=60)
              CGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCG                                                                                >  SRR4302537.5462702/1‑94 (MQ=60)
                ATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTC                                                                              >  SRR4302537.4439761/1‑94 (MQ=60)
                ATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTC                                                                              >  SRR4302537.1252201/1‑94 (MQ=60)
                ATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTC                                                                              >  SRR4302537.4804692/1‑94 (MQ=60)
                ATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTC                                                                              >  SRR4302537.5782287/1‑94 (MQ=60)
                ATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATATCTGCGCCCGTACCGACATCCAAAAGTTGAACCGTC                                                                              >  SRR4302537.2770124/1‑94 (MQ=60)
                  TGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCAAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGC                                                                            >  SRR4302537.4581684/1‑94 (MQ=60)
                   GAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCG                                                                           >  SRR4302537.242335/1‑94 (MQ=60)
                   GAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCG                                                                           >  SRR4302537.3356712/1‑94 (MQ=60)
                    AGTCCATCATCGAAGCCCGTAATAAAGGCGGCGACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGT                                                                          <  SRR4302537.238006/94‑1 (MQ=60)
                        CATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTG                                                                      <  SRR4302537.2889757/94‑1 (MQ=60)
                        CATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTG                                                                      <  SRR4302537.6050889/94‑1 (MQ=60)
                         ATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGG                                                                     >  SRR4302537.1653472/1‑94 (MQ=60)
                         ATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGG                                                                     <  SRR4302537.5255992/94‑1 (MQ=60)
                           CATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAA                                                                   <  SRR4302537.1449935/94‑1 (MQ=60)
                            ATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAANTTGAACCGTCGCGTGNTNGNAA                                                                  >  SRR4302537.5594029/1‑94 (MQ=60)
                            ATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAA                                                                  >  SRR4302537.5463930/1‑94 (MQ=60)
                            ATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAA                                                                  >  SRR4302537.1686350/1‑94 (MQ=60)
                              CGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAA                                                                >  SRR4302537.2137267/1‑94 (MQ=60)
                               GAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAAC                                                               <  SRR4302537.1248378/94‑1 (MQ=60)
                                AAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACT                                                              >  SRR4302537.3032836/1‑94 (MQ=60)
                                 AGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTG                                                             >  SRR4302537.3057085/1‑94 (MQ=60)
                                 AGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTG                                                             <  SRR4302537.371471/94‑1 (MQ=60)
                                 AGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTG                                                             <  SRR4302537.4963475/94‑1 (MQ=60)
                                  GCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGA                                                            <  SRR4302537.6132146/94‑1 (MQ=60)
                                  GCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGA                                                            >  SRR4302537.2670790/1‑94 (MQ=60)
                                   CCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGAT                                                           >  SRR4302537.1962557/1‑94 (MQ=60)
                                   CCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGAT                                                           <  SRR4302537.313275/94‑1 (MQ=60)
                                    CCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATC                                                          >  SRR4302537.5437194/1‑94 (MQ=60)
                                     CGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATGTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCA                                                         <  SRR4302537.2902458/94‑1 (MQ=60)
                                     CGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCA                                                         <  SRR4302537.1959761/94‑1 (MQ=60)
                                      GTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCAT                                                        >  SRR4302537.280225/1‑94 (MQ=60)
                                      GTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCAT                                                        <  SRR4302537.2429797/94‑1 (MQ=60)
                                      GTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCAT                                                        <  SRR4302537.267126/94‑1 (MQ=60)
                                       TAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATG                                                       >  SRR4302537.1153066/1‑94 (MQ=60)
                                       TAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATG                                                       <  SRR4302537.4192097/94‑1 (MQ=60)
                                         ATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTC                                                     >  SRR4302537.5129444/1‑94 (MQ=60)
                                         ATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTC                                                     >  SRR4302537.273946/1‑94 (MQ=60)
                                         ATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTC                                                     >  SRR4302537.4568921/1‑94 (MQ=60)
                                           AAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCG                                                   >  SRR4302537.5735509/1‑94 (MQ=60)
                                              GGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGG                                                <  SRR4302537.2100282/94‑1 (MQ=60)
                                                CGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCG                                              >  SRR4302537.5399540/1‑94 (MQ=60)
                                                  GCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTTGAAAAACTGATCATGTCCGGGGCGTT                                            <  SRR4302537.5663532/94‑1 (MQ=60)
                                                   CTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTT                                           >  SRR4302537.2059878/1‑94 (MQ=60)
                                                     ACTTCCGCGAACTGTTTTATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGA                                         <  SRR4302537.2790850/94‑1 (MQ=60)
                                                     ACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGA                                         >  SRR4302537.3800778/1‑94 (MQ=60)
                                                      CTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGAC                                        >  SRR4302537.3124834/1‑94 (MQ=60)
                                                       TTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACC                                       <  SRR4302537.6178801/94‑1 (MQ=60)
                                                       TTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACC                                       >  SRR4302537.1847728/1‑94 (MQ=60)
                                                       TTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACC                                       >  SRR4302537.861668/1‑94 (MQ=60)
                                                         CCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGT                                     >  SRR4302537.3535138/1‑94 (MQ=60)
                                                         CCGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGT                                     >  SRR4302537.2600333/1‑94 (MQ=60)
                                                         CCGCGAACGGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGgttgcccgc                                     >  SRR4302537.5664424/1‑85 (MQ=60)
                                                          CGCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTC                                    >  SRR4302537.2837973/1‑94 (MQ=60)
                                                           GCGAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCT                                   <  SRR4302537.3737729/94‑1 (MQ=60)
                                                             GAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTG                                 >  SRR4302537.3001943/1‑94 (MQ=60)
                                                             GAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTG                                 <  SRR4302537.3710754/94‑1 (MQ=60)
                                                             GAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTG                                 <  SRR4302537.6268646/94‑1 (MQ=60)
                                                             GAACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTG                                 <  SRR4302537.1428121/94‑1 (MQ=60)
                                                              AACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGG                                <  SRR4302537.6224836/94‑1 (MQ=60)
                                                              AACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGG                                <  SRR4302537.47868/94‑1 (MQ=60)
                                                               ACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTTGG                               >  SRR4302537.1487210/1‑94 (MQ=60)
                                                               ACTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGG                               <  SRR4302537.2066757/94‑1 (MQ=60)
                                                                CTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGC                              >  SRR4302537.5718116/1‑94 (MQ=60)
                                                                CTGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGC                              >  SRR4302537.6023697/1‑94 (MQ=60)
                                                                 TGTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCC                             <  SRR4302537.186042/94‑1 (MQ=60)
                                                                  GTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCA                            >  SRR4302537.5385743/1‑94 (MQ=60)
                                                                  GTTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCA                            >  SRR4302537.2614030/1‑94 (MQ=60)
                                                                   TTTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCAC                           >  SRR4302537.1825353/1‑94 (MQ=60)
                                                                    TTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACA                          >  SRR4302537.5503752/1‑94 (MQ=60)
                                                                    TTGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACA                          >  SRR4302537.2761564/1‑94 (MQ=60)
                                                                     TGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACAT                         >  SRR4302537.6355708/1‑94 (MQ=60)
                                                                     TGATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACAT                         >  SRR4302537.204165/1‑94 (MQ=60)
                                                                      GATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATC                        <  SRR4302537.3752399/94‑1 (MQ=60)
                                                                       ATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCG                       >  SRR4302537.2557654/1‑94 (MQ=60)
                                                                       ATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCG                       >  SRR4302537.716267/1‑94 (MQ=60)
                                                                       ATCTCTGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCG                       <  SRR4302537.4818810/94‑1 (MQ=60)
                                                                            TGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGTCACATCGCGCAG                  >  SRR4302537.2990864/1‑94 (MQ=60)
                                                                            TGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAG                  >  SRR4302537.4842727/1‑94 (MQ=60)
                                                                            TGCGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAG                  <  SRR4302537.5664553/94‑1 (MQ=60)
                                                                              CGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCG                >  SRR4302537.1191682/1‑94 (MQ=60)
                                                                              CGCCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCG                >  SRR4302537.4270116/1‑94 (MQ=60)
                                                                                CCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCT              <  SRR4302537.3309977/94‑1 (MQ=60)
                                                                                CCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCT              <  SRR4302537.6225076/94‑1 (MQ=60)
                                                                                CCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCT              >  SRR4302537.331494/1‑94 (MQ=60)
                                                                                CCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCT              <  SRR4302537.6196004/94‑1 (MQ=60)
                                                                                CCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCT              <  SRR4302537.3455969/94‑1 (MQ=60)
                                                                                CCCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCT              <  SRR4302537.5929594/94‑1 (MQ=60)
                                                                                 CCGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGCCTTGGGCCACATCGCGCAGCGCTG             <  SRR4302537.424056/94‑1 (MQ=60)
                                                                                  CGTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGA            >  SRR4302537.1935494/1‑94 (MQ=60)
                                                                                   GTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGAT           >  SRR4302537.4781083/1‑94 (MQ=60)
                                                                                   GTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGAT           <  SRR4302537.5665443/94‑1 (MQ=60)
                                                                                   GTACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGAT           >  SRR4302537.20002/1‑94 (MQ=60)
                                                                                    TACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATG          >  SRR4302537.5087381/1‑94 (MQ=60)
                                                                                     ACCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGA         >  SRR4302537.4784501/1‑94 (MQ=60)
                                                                                      CCGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAA        <  SRR4302537.4640119/94‑1 (MQ=60)
                                                                                      CCGACATCAAAAAGTTGAACCGCCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAA        >  SRR4302537.4889162/1‑94 (MQ=60)
                                                                                       CGACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAAC       >  SRR4302537.3709673/1‑94 (MQ=60)
                                                                                         ACATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTC     >  SRR4302537.2724939/1‑94 (MQ=60)
                                                                                           ATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGC   <  SRR4302537.4715583/94‑1 (MQ=60)
                                                                                           ATCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGC   <  SRR4302537.530831/94‑1 (MQ=60)
                                                                                            TCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCT  <  SRR4302537.5525549/94‑1 (MQ=60)
                                                                                            |                                                                                             
GGTCGGTGAAGGTCCGATTGAGGCCATCATCGAAGCCCGTAATAAAGGCGGCTACTTCCGCGAACTGTTTGATCTCTGCGCCCGTACCGACACCAAAAAGTTGAACCGTCGCGTGCTGGAAAAACTGATCATGTCCGGGGCGTTTGACCGTCTTGGGCCACATCGCGCAGCGCTGATGAACTCGCT  >  NC_000913/207645‑207830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑