Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 323,547 C→T A189V (GCC→GTC)  ykgG → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913323,5470CT99.1% 1664.8 / ‑3.0 449A189V (GCC→GTC) ykgGputative transporter
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base T (235/209);  total (238/211)
Fisher's exact test for biased strand distribution p-value = 6.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 458 total aligned reads displayed.

CTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGCCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTCATGG  >  NC_000913/323456‑323637
                                                                                           |                                                                                          
 tCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCgg                                                                                         <  1:7489269/94‑1 (MQ=255)
   cgggAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTg                                                                                       <  1:2998249/92‑1 (MQ=255)
    cGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGa                                                                                      >  1:4729276/1‑94 (MQ=255)
    cGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGa                                                                                      <  1:884009/94‑1 (MQ=255)
    cGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGa                                                                                      >  1:4235810/1‑94 (MQ=255)
      gAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAAc                                                                                    >  1:7567235/1‑94 (MQ=255)
      gAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAAc                                                                                    <  2:7970993/94‑1 (MQ=255)
      gAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAAc                                                                                    >  2:8395871/1‑94 (MQ=255)
       aaTATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACg                                                                                   <  1:5943608/94‑1 (MQ=255)
       aaTATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACg                                                                                   <  2:4353223/94‑1 (MQ=255)
       aaTATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACg                                                                                   >  2:9019163/1‑94 (MQ=255)
        atatTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGa                                                                                  >  2:9315360/1‑94 (MQ=255)
         tatTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTg                                                                                       <  1:4398195/88‑1 (MQ=255)
         tatTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGaa                                                                                 >  1:1641686/1‑94 (MQ=255)
         tatTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGaa                                                                                 <  2:6746097/94‑1 (MQ=255)
         tatTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGaa                                                                                 >  1:2979702/1‑94 (MQ=255)
           ttCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATg                                                                               <  1:10172330/94‑1 (MQ=255)
            tctcTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGc                                                                              >  1:7091512/1‑94 (MQ=255)
              tctTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCt                                                                            >  2:5822758/1‑94 (MQ=255)
               ctTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCtt                                                                           <  2:5655215/94‑1 (MQ=255)
               ctTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCtt                                                                           >  1:54198/1‑94 (MQ=255)
                tttttATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTc                                                                          >  2:1009987/1‑94 (MQ=255)
                 ttttATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTcc                                                                         <  1:1310308/94‑1 (MQ=255)
                  tttATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCt                                                                        >  2:9691984/1‑94 (MQ=255)
                   ttATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTg                                                                       <  2:1707685/94‑1 (MQ=255)
                   ttATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTg                                                                       >  2:6603230/1‑94 (MQ=255)
                    tATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGACCAGGTCGGTGAACGGATGCCTTCCTGc                                                                      >  1:2809796/1‑94 (MQ=255)
                      tCCTGCGTAAAAGCACTATGCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCAt                                                                    >  1:5779670/1‑94 (MQ=255)
                      tCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCAt                                                                    <  2:4439964/94‑1 (MQ=255)
                        cTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTa                                                                  <  2:4756302/94‑1 (MQ=255)
                        cTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTa                                                                  <  2:401206/94‑1 (MQ=255)
                        cTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTa                                                                  <  1:6843417/94‑1 (MQ=255)
                        cTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTa                                                                  >  1:6963033/1‑94 (MQ=255)
                         tGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTaa                                                                 <  2:8661621/94‑1 (MQ=255)
                          gCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGCAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAAc                                                                >  1:8184348/1‑94 (MQ=255)
                            gTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAAcat                                                              >  2:3874983/1‑94 (MQ=255)
                                 aGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTa                                                         >  2:4667960/1‑94 (MQ=255)
                                  gCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCTCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAg                                                        <  2:2303601/94‑1 (MQ=255)
                                  gCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAg                                                        <  2:499848/94‑1 (MQ=255)
                                   cACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGCATGCCTTCCTGCATTAATCTCATTAgg                                                       >  2:3574085/1‑93 (MQ=255)
                                   cACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGc                                                       >  1:3181066/1‑94 (MQ=255)
                                    ccTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCg                                                      <  2:3756507/93‑1 (MQ=255)
                                    aCTATCCTGCCGCGTGTAGCGCAACTTGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCg                                                      >  1:9374343/1‑94 (MQ=255)
                                    aCTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCg                                                      <  2:2980097/94‑1 (MQ=255)
                                     cTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCgg                                                     <  1:6133790/94‑1 (MQ=255)
                                       aTCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGcc                                                   >  1:2133651/1‑94 (MQ=255)
                                         ccTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGcccc                                                 >  1:4001969/1‑94 (MQ=255)
                                         ccTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATACCTTGCTGCATTAACATCATTAGCGGcctc                                                 >  1:10514619/1‑92 (MQ=255)
                                          cTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCa                                                >  2:737919/1‑94 (MQ=255)
                                          cTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCa                                                >  1:5742786/1‑94 (MQ=255)
                                          cTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCa                                                >  2:8851927/1‑94 (MQ=255)
                                           tGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAg                                               <  1:8335969/94‑1 (MQ=255)
                                           tGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAg                                               >  1:2340597/1‑94 (MQ=255)
                                           tGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAg                                               >  1:4963665/1‑94 (MQ=255)
                                           tGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAg                                               <  2:8046205/94‑1 (MQ=255)
                                           tGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAg                                               <  2:2073034/94‑1 (MQ=255)
                                            gCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGt                                              <  2:5441896/94‑1 (MQ=255)
                                            gCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGt                                              <  1:7220608/94‑1 (MQ=255)
                                             ccGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGtt                                             <  1:1136190/94‑1 (MQ=255)
                                              cgcgTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTc                                            >  2:2431937/1‑94 (MQ=255)
                                                 gtgtAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAAc                                         >  1:7729036/1‑94 (MQ=255)
                                                 gtgtAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGACCCAGTTCAAc                                         >  1:615361/1‑94 (MQ=255)
                                                  tgtAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAAcg                                        >  1:1238774/1‑94 (MQ=255)
                                                   gtAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAAcgg                                       >  1:2685029/1‑94 (MQ=255)
                                                     aGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAAcggcg                                     <  2:2001262/94‑1 (MQ=255)
                                                      gcgcAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAAcggcgg                                    <  2:5253052/94‑1 (MQ=255)
                                                      gcgcAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAAcggcgg                                    <  1:2554839/94‑1 (MQ=255)
                                                        gcAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGat                                  <  2:9450120/94‑1 (MQ=255)
                                                        gcAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGCCGGat                                  <  1:9045012/94‑1 (MQ=255)
                                                           aCTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATAtt                               >  2:330540/1‑94 (MQ=255)
                                                            cTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTg                              >  2:7692977/1‑94 (MQ=255)
                                                              cGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAg                            >  2:92023/1‑94 (MQ=255)
                                                               gCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGc                           <  2:2800771/94‑1 (MQ=255)
                                                               gCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGc                           <  2:5062816/94‑1 (MQ=255)
                                                               gCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGc                           >  1:7910259/1‑94 (MQ=255)
                                                               gCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCATCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGc                           <  1:3493896/94‑1 (MQ=255)
                                                                cAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCt                          >  2:1465796/1‑94 (MQ=255)
                                                                cAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCt                          >  2:647440/1‑94 (MQ=255)
                                                                  gAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCATTTCAACGGCGGATATTGAGCTTa                        >  1:5842951/1‑94 (MQ=255)
                                                                   aaaaaTTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTAt                       <  2:7654941/94‑1 (MQ=255)
                                                                       aTTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCaaa                   >  1:9927022/1‑94 (MQ=255)
                                                                        ttGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAg                  <  1:3579885/94‑1 (MQ=255)
                                                                        ttGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAg                  <  2:6153591/94‑1 (MQ=255)
                                                                           cATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAgtcg               <  2:4865380/94‑1 (MQ=255)
                                                                           cATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAgtcg               >  2:4129047/1‑94 (MQ=255)
                                                                           cATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAgtcg               >  1:6479327/1‑94 (MQ=255)
                                                                            aTCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAgtcgt              <  2:2596244/94‑1 (MQ=255)
                                                                            aTCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAgtcgt              >  2:6927709/1‑94 (MQ=255)
                                                                              cAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTTTAAAAGtggtcg            >  1:3874977/1‑94 (MQ=255)
                                                                               aGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCgg           >  2:5968564/1‑94 (MQ=255)
                                                                               aGAAAGCGCAGGCCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCgg           >  1:6596709/1‑94 (MQ=255)
                                                                                gAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGa          <  1:8657373/94‑1 (MQ=255)
                                                                                    gcgcAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTc      <  1:10083940/94‑1 (MQ=255)
                                                                                    gcgcAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTc      >  2:3123461/1‑94 (MQ=255)
                                                                                      gcAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTCAt    <  2:34128/94‑1 (MQ=255)
                                                                                      gcAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTCAt    >  2:3217502/1‑94 (MQ=255)
                                                                                      gcAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTCAt    <  2:1051830/94‑1 (MQ=255)
                                                                                       cAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTCATg   >  1:10333135/1‑94 (MQ=255)
                                                                                        aGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTCATgg  >  1:1820969/1‑94 (MQ=255)
                                                                                        aGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTCATgg  >  2:121256/1‑94 (MQ=255)
                                                                                           |                                                                                          
CTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGCCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTCATGG  >  NC_000913/323456‑323637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 236 total aligned reads displayed.
TGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGCCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTCATGG  >  NC_000913/323454‑323637
                                                                                             |                                                                                          
TGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGT                                                                                            >  SRR4302545.3654507/1‑94 (MQ=60)
 GCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTC                                                                                           <  SRR4302545.3065243/94‑1 (MQ=60)
 GCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTC                                                                                           <  SRR4302545.2741005/94‑1 (MQ=60)
 GCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTC                                                                                           >  SRR4302545.2415836/1‑94 (MQ=60)
 GCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTC                                                                                           >  SRR4302545.3425100/1‑94 (MQ=60)
 GCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTC                                                                                           >  SRR4302545.6010729/1‑94 (MQ=60)
  CTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCG                                                                                          >  SRR4302545.8871833/1‑94 (MQ=60)
  CTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCG                                                                                          <  SRR4302545.6872422/94‑1 (MQ=60)
   TCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGG                                                                                         <  SRR4302545.7701237/94‑1 (MQ=60)
    CCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGT                                                                                        >  SRR4302545.10055777/1‑94 (MQ=60)
     tgatctTATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTG                                                                                       <  SRR4302545.4509706/88‑1 (MQ=60)
     CGGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTG                                                                                       <  SRR4302545.3071372/94‑1 (MQ=60)
     CCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTG                                                                                       <  SRR4302545.3278100/94‑1 (MQ=60)
      CGGAATATTCTCTTTTTATCCTGCGTAAAATCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGA                                                                                      <  SRR4302545.6128316/94‑1 (MQ=60)
      CGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGA                                                                                      <  SRR4302545.596878/94‑1 (MQ=60)
      CGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGA                                                                                      >  SRR4302545.2004350/1‑94 (MQ=60)
      CGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGA                                                                                      >  SRR4302545.4341724/1‑94 (MQ=60)
      CGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGA                                                                                      >  SRR4302545.4341723/1‑94 (MQ=60)
      CGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGA                                                                                      <  SRR4302545.588118/94‑1 (MQ=60)
       GGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCGGAAAGCGCAGGTCGGTGAA                                                                                     <  SRR4302545.6074053/94‑1 (MQ=60)
       GGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAA                                                                                     >  SRR4302545.8792847/1‑94 (MQ=60)
       GGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAA                                                                                     >  SRR4302545.4277372/1‑94 (MQ=60)
        GAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAAC                                                                                    >  SRR4302545.292529/1‑94 (MQ=60)
        GAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAAC                                                                                    >  SRR4302545.7781116/1‑94 (MQ=60)
         AATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACG                                                                                   >  SRR4302545.1260640/1‑94 (MQ=60)
         AATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACG                                                                                   <  SRR4302545.6103968/94‑1 (MQ=60)
         AATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGGCGGTGAACG                                                                                   <  SRR4302545.1813373/94‑1 (MQ=60)
          ATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGACCGA                                                                                  >  SRR4302545.9116516/1‑94 (MQ=60)
          ATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGA                                                                                  >  SRR4302545.9116515/1‑94 (MQ=60)
           TATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAA                                                                                 >  SRR4302545.1311958/1‑94 (MQ=60)
           TATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAA                                                                                 <  SRR4302545.2620573/94‑1 (MQ=60)
           TATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAA                                                                                 >  SRR4302545.1680611/1‑94 (MQ=60)
           TATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAA                                                                                 >  SRR4302545.3588492/1‑94 (MQ=60)
            ATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAAT                                                                                <  SRR4302545.366793/94‑1 (MQ=60)
            ATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAAT                                                                                <  SRR4302545.2850198/94‑1 (MQ=60)
            ATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAAT                                                                                >  SRR4302545.3633203/1‑94 (MQ=60)
            ATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAAT                                                                                >  SRR4302545.4656757/1‑94 (MQ=60)
            ATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAAT                                                                                <  SRR4302545.1732153/94‑1 (MQ=60)
            ATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCCCGGGTACCGCACTCCGACGAAAAATTGCTTCAGAAAGCGCAGGCCGGTGAACGAtg                                                                                >  SRR4302545.6503616/1‑92 (MQ=49)
             TTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATG                                                                               <  SRR4302545.10473034/94‑1 (MQ=60)
              TCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGC                                                                              >  SRR4302545.7287894/1‑94 (MQ=60)
                TCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCT                                                                            >  SRR4302545.9091481/1‑94 (MQ=60)
                TCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCT                                                                            <  SRR4302545.5956992/94‑1 (MQ=60)
                TCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCA                                                                            <  SRR4302545.4582652/94‑1 (MQ=60)
                 CTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTT                                                                           >  SRR4302545.55426/1‑94 (MQ=60)
                 CTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTT                                                                           >  SRR4302545.10484968/1‑94 (MQ=60)
                 CTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTT                                                                           >  SRR4302545.10472982/1‑94 (MQ=60)
                   TTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCC                                                                         >  SRR4302545.4443675/1‑94 (MQ=60)
                   TTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCC                                                                         <  SRR4302545.1339394/94‑1 (MQ=60)
                    TTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCT                                                                        >  SRR4302545.4522947/1‑94 (MQ=60)
                     TTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTG                                                                       >  SRR4302545.10251640/1‑94 (MQ=60)
                     TTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTG                                                                       >  SRR4302545.7743698/1‑94 (MQ=60)
                     TTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTG                                                                       <  SRR4302545.6303142/94‑1 (MQ=60)
                     TTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTG                                                                       <  SRR4302545.4847811/94‑1 (MQ=60)
                      TATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGC                                                                      >  SRR4302545.4033322/1‑94 (MQ=60)
                      TATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGC                                                                      >  SRR4302545.6724247/1‑94 (MQ=60)
                      TATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGC                                                                      >  SRR4302545.9176106/1‑94 (MQ=60)
                      TATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGACCAGGTCGGTGAACGGATGCCTTCCTGC                                                                      >  SRR4302545.2877804/1‑94 (MQ=60)
                        TCCTGCGTAAAAGCACTATGCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCAT                                                                    >  SRR4302545.5935052/1‑94 (MQ=60)
                        TCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCAT                                                                    <  SRR4302545.3451395/94‑1 (MQ=60)
                        TCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCAT                                                                    >  SRR4302545.4954073/1‑94 (MQ=60)
                        TCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCAT                                                                    >  SRR4302545.1902878/1‑94 (MQ=60)
                         CCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATT                                                                   >  SRR4302545.9649447/1‑94 (MQ=60)
                         CCTGCGTAAAAGCACTATCCTGACGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATT                                                                   >  SRR4302545.10741659/1‑94 (MQ=60)
                          CTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTA                                                                  >  SRR4302545.7154995/1‑94 (MQ=60)
                          CTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTA                                                                  <  SRR4302545.7031383/94‑1 (MQ=60)
                          CTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTA                                                                  >  SRR4302545.10916355/1‑94 (MQ=60)
                            GCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGCAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAAC                                                                >  SRR4302545.8421139/1‑94 (MQ=60)
                            GCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAAC                                                                >  SRR4302545.1224071/1‑94 (MQ=60)
                            GCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAAC                                                                >  SRR4302545.2951739/1‑94 (MQ=60)
                             CGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACA                                                               >  SRR4302545.6282877/1‑94 (MQ=60)
                             CGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACA                                                               >  SRR4302545.214622/1‑94 (MQ=60)
                             CGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACA                                                               >  SRR4302545.6282878/1‑94 (MQ=60)
                             CGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCCGCATTAACA                                                               >  SRR4302545.2713033/1‑94 (MQ=60)
                              GTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACAT                                                              >  SRR4302545.6407071/1‑94 (MQ=60)
                               TAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATC                                                             <  SRR4302545.297198/94‑1 (MQ=60)
                                AGAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCA                                                            <  SRR4302545.509838/94‑1 (MQ=60)
                                AAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCA                                                            >  SRR4302545.4050427/1‑94 (MQ=60)
                                AAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCA                                                            >  SRR4302545.305509/1‑94 (MQ=60)
                                 AAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCAT                                                           <  SRR4302545.6038158/94‑1 (MQ=60)
                                 AAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCAT                                                           <  SRR4302545.6295705/94‑1 (MQ=60)
                                 AAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCAT                                                           <  SRR4302545.3650410/94‑1 (MQ=60)
                                 AAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCAT                                                           <  SRR4302545.3069134/94‑1 (MQ=60)
                                  AAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATT                                                          <  SRR4302545.10938182/94‑1 (MQ=60)
                                   AGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTA                                                         <  SRR4302545.1294425/94‑1 (MQ=60)
                                     CACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGC                                                       >  SRR4302545.3259116/1‑94 (MQ=60)
                                     CACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGC                                                       >  SRR4302545.8427045/1‑94 (MQ=60)
                                      ACTATCCTGCCGCGTGTAGCGCAACTTGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCG                                                      >  SRR4302545.9649408/1‑94 (MQ=60)
                                      ACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCG                                                      <  SRR4302545.8158165/94‑1 (MQ=60)
                                      ACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCG                                                      >  SRR4302545.2100992/1‑94 (MQ=60)
                                      ACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCG                                                      <  SRR4302545.2290152/94‑1 (MQ=60)
                                      ACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCG                                                      <  SRR4302545.2316158/94‑1 (MQ=60)
                                      ACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCG                                                      <  SRR4302545.7432337/94‑1 (MQ=60)
                                       CTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGG                                                     <  SRR4302545.6300572/94‑1 (MQ=60)
                                       CTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGG                                                     <  SRR4302545.6300571/94‑1 (MQ=60)
                                        TATCCTGCTGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGC                                                    <  SRR4302545.1344600/94‑1 (MQ=60)
                                        TATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGC                                                    >  SRR4302545.671931/1‑94 (MQ=60)
                                        TATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGC                                                    >  SRR4302545.5798707/1‑94 (MQ=60)
                                         ATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGACTGCCTTCCTGCATTAACCTCATTAGCGGCC                                                   >  SRR4302545.3131759/1‑94 (MQ=60)
                                         ATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCC                                                   >  SRR4302545.3131760/1‑94 (MQ=60)
                                         ATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCC                                                   >  SRR4302545.2185965/1‑94 (MQ=60)
                                         ATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCC                                                   >  SRR4302545.828168/1‑94 (MQ=60)
                                          TCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCC                                                  >  SRR4302545.3185240/1‑94 (MQ=60)
                                          TCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCC                                                  <  SRR4302545.10838730/94‑1 (MQ=60)
                                          TCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCC                                                  >  SRR4302545.7565734/1‑94 (MQ=60)
                                           CCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCC                                                 >  SRR4302545.4102059/1‑94 (MQ=60)
                                           CCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATACCTTGCTGCATTAACATCATTAGCGGCCTC                                                 >  SRR4302545.10824453/1‑94 (MQ=60)
                                            CTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCA                                                >  SRR4302545.3861498/1‑94 (MQ=60)
                                            CTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCA                                                >  SRR4302545.5896990/1‑94 (MQ=60)
                                            CTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCA                                                >  SRR4302545.2845071/1‑94 (MQ=60)
                                             TGCCGCGTGTAGCGCAACTGGAAGAAAAATTTCATCAGAAAGCGCAGGTCGGTGAACGAATGCTTTCCTGCTTTAACTTCATTAGCGCCCCCGG                                               >  SRR4302545.8748487/1‑94 (MQ=60)
                                             TGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAG                                               >  SRR4302545.2397581/1‑94 (MQ=60)
                                             TGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAG                                               >  SRR4302545.8927425/1‑94 (MQ=60)
                                             TGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAG                                               <  SRR4302545.8577516/94‑1 (MQ=60)
                                             TGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAG                                               >  SRR4302545.5089385/1‑94 (MQ=60)
                                             TGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCCCAGGTCGGTGAACGCATGCCTTCCTGCATTAACATCACTAGCGGCCCCAG                                               >  SRR4302545.2998380/1‑94 (MQ=60)
                                              GCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGT                                              >  SRR4302545.7110539/1‑94 (MQ=60)
                                              GCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGT                                              <  SRR4302545.9473860/94‑1 (MQ=60)
                                              GCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGT                                              <  SRR4302545.889159/94‑1 (MQ=60)
                                              GCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGT                                              <  SRR4302545.7422007/94‑1 (MQ=60)
                                              GCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGT                                              <  SRR4302545.7422006/94‑1 (MQ=60)
                                               gttgaactgggccgctaatgatgttAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTT                                             >  SRR4302545.8781202/26‑94 (MQ=60)
                                               CCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTT                                             <  SRR4302545.8626817/94‑1 (MQ=60)
                                               CCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTT                                             >  SRR4302545.8247050/1‑94 (MQ=60)
                                               CCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTT                                             <  SRR4302545.1161060/94‑1 (MQ=60)
                                               CCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTT                                             >  SRR4302545.10552052/1‑94 (MQ=60)
                                                CGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTC                                            <  SRR4302545.8667279/94‑1 (MQ=60)
                                                  CGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAA                                          >  SRR4302545.6774853/1‑94 (MQ=60)
                                                  CGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAA                                          >  SRR4302545.7014841/1‑94 (MQ=60)
                                                   GTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAAC                                         <  SRR4302545.6803262/94‑1 (MQ=60)
                                                   GTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAAC                                         >  SRR4302545.7947806/1‑94 (MQ=60)
                                                    TGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACG                                        >  SRR4302545.1266690/1‑94 (MQ=60)
                                                     GTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGG                                       >  SRR4302545.2749716/1‑94 (MQ=60)
                                                      TAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGC                                      <  SRR4302545.2343690/94‑1 (MQ=60)
                                                      TAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGC                                      >  SRR4302545.5977897/1‑94 (MQ=60)
                                                      TAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGC                                      >  SRR4302545.7931932/1‑94 (MQ=60)
                                                       AGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCG                                     <  SRR4302545.5923400/94‑1 (MQ=60)
                                                       AGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCG                                     <  SRR4302545.5075592/94‑1 (MQ=60)
                                                        GCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGG                                    <  SRR4302545.2616463/94‑1 (MQ=60)
                                                         CGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGA                                   >  SRR4302545.586312/1‑94 (MQ=60)
                                                          GCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTTGCGGCCCCAGTTCACCGGCGGAT                                  >  SRR4302545.7795378/1‑94 (MQ=60)
                                                          GCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGAT                                  >  SRR4302545.7795631/1‑94 (MQ=60)
                                                          GCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGAT                                  <  SRR4302545.8576428/94‑1 (MQ=60)
                                                          GCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGAT                                  <  SRR4302545.6317019/94‑1 (MQ=60)
                                                          GCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGCCGGAT                                  <  SRR4302545.9308835/94‑1 (MQ=60)
                                                            AACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATAT                                >  SRR4302545.5195391/1‑94 (MQ=60)
                                                            AACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATAT                                >  SRR4302545.8049512/1‑94 (MQ=60)
                                                            AACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATAT                                >  SRR4302545.8049510/1‑94 (MQ=60)
                                                             ACTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATT                               <  SRR4302545.1218537/94‑1 (MQ=60)
                                                              CTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTG                              <  SRR4302545.154753/94‑1 (MQ=60)
                                                              CTCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTG                              <  SRR4302545.3997354/94‑1 (MQ=60)
                                                               TCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGA                             <  SRR4302545.2836502/94‑1 (MQ=60)
                                                               TCGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGA                             <  SRR4302545.4219571/94‑1 (MQ=60)
                                                                CGCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAG                            <  SRR4302545.2403138/94‑1 (MQ=60)
                                                                 GCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGC                           <  SRR4302545.259938/94‑1 (MQ=60)
                                                                 GCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGC                           >  SRR4302545.8134822/1‑94 (MQ=60)
                                                                 GCAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCATCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGC                           <  SRR4302545.3579563/94‑1 (MQ=60)
                                                                  CAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCT                          <  SRR4302545.9513019/94‑1 (MQ=60)
                                                                  CAGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCT                          >  SRR4302545.4380647/1‑94 (MQ=60)
                                                                   AGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTT                         <  SRR4302545.5490516/94‑1 (MQ=60)
                                                                   AGAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTT                         >  SRR4302545.5673287/1‑94 (MQ=60)
                                                                    GAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCATTTCAACGGCGGATATTGAGCTTA                        >  SRR4302545.6000279/1‑94 (MQ=60)
                                                                    GAAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTA                        >  SRR4302545.7752132/1‑94 (MQ=60)
                                                                     AAAAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTTAGCTTAT                       >  SRR4302545.742152/1‑94 (MQ=60)
                                                                       AAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCA                     >  SRR4302545.10257408/1‑94 (MQ=60)
                                                                       AAATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCA                     <  SRR4302545.9067161/94‑1 (MQ=60)
                                                                        AATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAA                    <  SRR4302545.4935777/94‑1 (MQ=60)
                                                                         ATTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAA                   >  SRR4302545.10221835/1‑94 (MQ=60)
                                                                          TTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAG                  <  SRR4302545.3667276/94‑1 (MQ=60)
                                                                          TTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAG                  >  SRR4302545.2654801/1‑94 (MQ=60)
                                                                          TTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAG                  >  SRR4302545.3682443/1‑94 (MQ=60)
                                                                          TTGCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAG                  <  SRR4302545.5919424/94‑1 (MQ=60)
                                                                            GCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTC                <  SRR4302545.7139434/94‑1 (MQ=60)
                                                                            GCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTC                <  SRR4302545.7119633/94‑1 (MQ=60)
                                                                            GCATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTC                <  SRR4302545.7261687/94‑1 (MQ=60)
                                                                             CATCAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCG               >  SRR4302545.6656468/1‑94 (MQ=60)
                                                                             CATCAAAAAGTGCAGTTCGGTGAACGAACGCCTTCCTGTATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGGCG               <  SRR4302545.7110579/94‑1 (MQ=60)
                                                                                CAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTTTAAAAGTGGTCG            >  SRR4302545.3971692/1‑94 (MQ=60)
                                                                                CAGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCG            >  SRR4302545.3026434/1‑94 (MQ=60)
                                                                                 AGAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGG           >  SRR4302545.5661955/1‑94 (MQ=60)
                                                                                 AGAAAGCGCAGGCCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGG           >  SRR4302545.6777295/1‑94 (MQ=60)
                                                                                  GAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGG          >  SRR4302545.8080101/1‑94 (MQ=60)
                                                                                  GAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGA          <  SRR4302545.2601274/94‑1 (MQ=60)
                                                                                  GAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGA          <  SRR4302545.1853901/94‑1 (MQ=60)
                                                                                  GAAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGA          <  SRR4302545.8908511/94‑1 (MQ=60)
                                                                                   AAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAG         >  SRR4302545.3062975/1‑94 (MQ=60)
                                                                                   AAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAG         <  SRR4302545.3200275/94‑1 (MQ=60)
                                                                                   AAAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAG         >  SRR4302545.6633124/1‑94 (MQ=60)
                                                                                    AAGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGT        >  SRR4302545.729564/1‑94 (MQ=60)
                                                                                     AGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGTACTTATCAAACTCCTCGGAGTT       >  SRR4302545.1301488/1‑94 (MQ=60)
                                                                                     AGCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTT       <  SRR4302545.9506807/94‑1 (MQ=60)
                                                                                      GCGCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTC      <  SRR4302545.10382209/94‑1 (MQ=60)
                                                                                       CGCAGGTCGGTGGACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTCA     >  SRR4302545.10756405/1‑94 (MQ=60)
                                                                                       CGCAGGCCGGGGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTCA     >  SRR4302545.10446924/1‑94 (MQ=60)
                                                                                        GCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTCAT    >  SRR4302545.7331666/1‑94 (MQ=60)
                                                                                        GCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTCAT    <  SRR4302545.10400206/94‑1 (MQ=60)
                                                                                        GCAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTCAT    >  SRR4302545.7331665/1‑94 (MQ=60)
                                                                                         CAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTCATG   >  SRR4302545.7191940/1‑94 (MQ=60)
                                                                                         CAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTCATG   >  SRR4302545.1678708/1‑94 (MQ=60)
                                                                                         CAGGTCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTCATG   >  SRR4302545.10637771/1‑94 (MQ=60)
                                                                                             |                                                                                          
TGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGCCGGTGAACGAATGCCTTCCTGCATTAACATCATTAGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTCATGG  >  NC_000913/323454‑323637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑