Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 588,625 A→G V518A (GTA→GCA)  nfrA ← NfrA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913588,6250AG98.5% 924.2 / ‑2.0 273V518A (GTA→GCA) nfrANfrA
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base G (139/130);  total (141/132)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 279 total aligned reads displayed.

GCAGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTACCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACAC  >  NC_000913/588533‑588714
                                                                                            |                                                                                         
 caGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGcc                                                                                         >  1:5612098/1‑94 (MQ=255)
  aGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCg                                                                                        >  1:4534034/1‑94 (MQ=255)
  aGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCg                                                                                        >  1:1215316/1‑94 (MQ=255)
    aTCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCa                                                                                      >  2:6315525/1‑94 (MQ=255)
     tCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGCTAGGTTGCCGCAc                                                                                     >  1:2313814/1‑94 (MQ=255)
      ccTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACg                                                                                    <  2:2030811/94‑1 (MQ=255)
      ccTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAAGCCGCCAGTGCGGTCGCGTAGTCCTCAACCTGATACGCCTGATAGGCGGCCGCACg                                                                                    >  1:5785080/1‑94 (MQ=255)
       cTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGa                                                                                   <  2:590139/94‑1 (MQ=255)
       cTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGa                                                                                   >  2:8193722/1‑94 (MQ=255)
         cATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATg                                                                                 <  2:2122746/94‑1 (MQ=255)
          aTTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGt                                                                                <  1:7659039/94‑1 (MQ=255)
           ttGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGtt                                                                               <  2:97652/94‑1 (MQ=255)
            tGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTg                                                                              <  1:2951741/94‑1 (MQ=255)
            tGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTg                                                                              <  2:8742575/94‑1 (MQ=255)
            tGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTg                                                                              <  2:3032518/94‑1 (MQ=255)
               tCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCa                                                                           <  2:413715/94‑1 (MQ=255)
               tCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCa                                                                           >  2:2642023/1‑94 (MQ=255)
                 aTGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAgg                                                                         >  2:5786906/1‑94 (MQ=255)
                   gTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGgcg                                                                       <  1:2525516/94‑1 (MQ=255)
                   gTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGgcg                                                                       <  2:119940/94‑1 (MQ=255)
                    tCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCACCCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGgcgc                                                                      <  1:6458426/94‑1 (MQ=255)
                     cGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCt                                                                     >  1:3960581/1‑94 (MQ=255)
                      gTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTc                                                                    >  1:1725349/1‑94 (MQ=255)
                           gACTGATTTTCTGCCAGGCCGCAAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTg                                                               <  1:10420043/94‑1 (MQ=255)
                             cTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTc                                                             >  2:9587625/1‑94 (MQ=255)
                              tGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCg                                                            >  2:1545322/1‑94 (MQ=255)
                              tGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCg                                                            >  1:326813/1‑94 (MQ=255)
                               taTTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCATGCGCTCAGTTGTCgt                                                           <  1:9042212/93‑1 (MQ=255)
                               gATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCgt                                                           <  2:5231364/94‑1 (MQ=255)
                                aTTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCgtt                                                          <  2:5103908/94‑1 (MQ=255)
                                aTTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCgtt                                                          >  2:6202584/1‑94 (MQ=255)
                                aTTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCgtt                                                          >  2:8519959/1‑94 (MQ=255)
                                   ttCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCgttgtt                                                       >  2:10582818/1‑94 (MQ=255)
                                    tCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCCCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTc                                                      <  1:6879193/94‑1 (MQ=255)
                                     cTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCg                                                     <  2:10255354/94‑1 (MQ=255)
                                      tGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCgg                                                    >  1:6808382/1‑94 (MQ=255)
                                       gCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGc                                                   <  1:8257129/94‑1 (MQ=255)
                                        ccAGGTCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGcc                                                  >  1:1056088/1‑94 (MQ=255)
                                        ccAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGcc                                                  >  1:4736535/1‑94 (MQ=255)
                                        ccAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGcc                                                  >  1:4366914/1‑94 (MQ=255)
                                        ccAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGcc                                                  >  1:9178613/1‑94 (MQ=255)
                                          aGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTg                                                <  2:7505054/94‑1 (MQ=255)
                                          aGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTg                                                >  2:10530841/1‑94 (MQ=255)
                                             ccgccAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAg                                             <  2:6855456/94‑1 (MQ=255)
                                             ccgccAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAg                                             <  2:1267372/94‑1 (MQ=255)
                                             ccgccAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTACCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAg                                             <  2:5284963/94‑1 (MQ=255)
                                              cgccAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGc                                            >  1:2355759/1‑94 (MQ=255)
                                                ccAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCa                                          >  2:3816349/1‑94 (MQ=255)
                                                ccAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCa                                          >  1:6633195/1‑94 (MQ=255)
                                                 cAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCAt                                         >  2:9196779/1‑94 (MQ=255)
                                                  aGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATg                                        >  2:7744749/1‑94 (MQ=255)
                                                  aGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATg                                        <  2:328551/94‑1 (MQ=255)
                                                   gTGCGGTGGCGTAGTCCTCAACCTGATACGCTTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGTTTGTCGTTGTTCGGCCTGAAGCCATGc                                       <  1:1471827/94‑1 (MQ=255)
                                                   gTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGc                                       <  2:4820866/94‑1 (MQ=255)
                                                   gTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGc                                       <  2:4375292/94‑1 (MQ=255)
                                                    tGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCa                                      >  1:3458765/1‑94 (MQ=255)
                                                     gCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCAt                                     >  2:1501306/1‑94 (MQ=255)
                                                     gCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGGTGCCAGGCGCTCGGTTGTCGTTGGTCGGCCCGAAGCCATGCAt                                     >  1:9406909/1‑94 (MQ=255)
                                                      cGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATa                                    >  2:6689719/1‑94 (MQ=255)
                                                      cGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATa                                    <  1:7893883/94‑1 (MQ=255)
                                                      cGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATa                                    <  1:348547/94‑1 (MQ=255)
                                                        gTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACa                                  >  1:8875940/1‑94 (MQ=255)
                                                         tGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACaa                                 <  1:9795439/94‑1 (MQ=255)
                                                          ggCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAAc                                <  2:7094421/94‑1 (MQ=255)
                                                            cGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGc                              <  2:5442904/94‑1 (MQ=255)
                                                            cGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTGCGGCCTGAAGCCATGCATACAACGc                              <  1:5635326/94‑1 (MQ=255)
                                                             gTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGcc                             >  2:4897702/1‑94 (MQ=255)
                                                             gTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGcc                             <  2:3498085/94‑1 (MQ=255)
                                                             cttgTCCTCAACCTGATACGCCTGATAGGCTCCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGcc                             <  1:1808351/91‑1 (MQ=255)
                                                               aGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCcac                           >  2:2396568/1‑94 (MQ=255)
                                                               aGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCcac                           >  2:8994443/1‑94 (MQ=255)
                                                                gTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCcaca                          >  1:3179789/1‑94 (MQ=255)
                                                                 tCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCacac                         >  2:8012304/1‑94 (MQ=255)
                                                                 tCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCacac                         >  1:10087581/1‑94 (MQ=255)
                                                                   cTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCg                       <  2:1283108/94‑1 (MQ=255)
                                                                   cTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCg                       >  1:5466085/1‑94 (MQ=255)
                                                                     cAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGCAGCCATGCATACAACGCCACACCggg                     >  2:318891/1‑94 (MQ=255)
                                                                     cAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCggg                     >  2:2012600/1‑94 (MQ=255)
                                                                     cAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCggg                     <  1:7501268/94‑1 (MQ=255)
                                                                     cAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCggg                     <  1:10009804/94‑1 (MQ=255)
                                                                     cAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGAGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCggg                     <  2:1783214/94‑1 (MQ=255)
                                                                      aaCCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGt                    >  1:9144321/1‑94 (MQ=255)
                                                                      aaCCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGt                    <  2:164478/94‑1 (MQ=255)
                                                                       aCCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTa                   <  1:3897076/94‑1 (MQ=255)
                                                                        ccTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAg                  <  2:3551792/94‑1 (MQ=255)
                                                                          tGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCg                <  1:4931729/94‑1 (MQ=255)
                                                                           gATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATAAAACGCCACACCGGGTAGCgg               >  1:6242292/1‑93 (MQ=255)
                                                                            aTACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCgtg              >  1:7303796/1‑94 (MQ=255)
                                                                            aTACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCgtg              >  2:1424816/1‑94 (MQ=255)
                                                                             tACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCgtgt             <  1:855484/94‑1 (MQ=255)
                                                                             tACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCgtgt             <  1:4068212/94‑1 (MQ=255)
                                                                                 ccTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCg         <  1:3559003/94‑1 (MQ=255)
                                                                                   tGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGAt       >  1:2600221/1‑94 (MQ=255)
                                                                                    gATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATa      >  2:8839746/1‑94 (MQ=255)
                                                                                    gATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATa      <  2:10066964/94‑1 (MQ=255)
                                                                                     aTAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATaa     <  2:431716/94‑1 (MQ=255)
                                                                                     aTAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATaa     <  2:5654005/94‑1 (MQ=255)
                                                                                      tAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAac    <  1:1569787/94‑1 (MQ=255)
                                                                                       aGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAaca   >  1:5232604/1‑94 (MQ=255)
                                                                                        ggCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAacac  >  2:8363074/1‑94 (MQ=255)
                                                                                            |                                                                                         
GCAGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTACCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACAC  >  NC_000913/588533‑588714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCAGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTACCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTTTG  >  NC_000913/588532‑588718
                                                                                             |                                                                                             
AGCAGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTG                                                                                               >  SRR4302545.767937/1‑94 (MQ=60)
AGCAGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTG                                                                                               <  SRR4302545.7880715/94‑1 (MQ=60)
 GCAGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGC                                                                                              >  SRR4302545.1992346/1‑94 (MQ=60)
  CAGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCC                                                                                             <  SRR4302545.36201/94‑1 (MQ=60)
  CAGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCC                                                                                             >  SRR4302545.5761099/1‑94 (MQ=60)
   AGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGCTAGGCTGCCG                                                                                            >  SRR4302545.4650236/1‑94 (MQ=60)
   AGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCG                                                                                            >  SRR4302545.4650237/1‑94 (MQ=60)
   AGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCG                                                                                            >  SRR4302545.10758562/1‑94 (MQ=60)
   AGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCG                                                                                            >  SRR4302545.1242866/1‑94 (MQ=60)
    GATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGC                                                                                           >  SRR4302545.8912363/1‑94 (MQ=60)
     ATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCA                                                                                          <  SRR4302545.5670139/94‑1 (MQ=60)
      TCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGCTAGGTTGCCGCAC                                                                                         >  SRR4302545.2370192/1‑94 (MQ=60)
      TCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCAC                                                                                         <  SRR4302545.4724915/94‑1 (MQ=60)
       CCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACG                                                                                        >  SRR4302545.1030747/1‑94 (MQ=60)
       CCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAAGCCGCCAGTGCGGTCGCGTAGTCCTCAACCTGATACGCCTGATAGGCGGCCGCACG                                                                                        >  SRR4302545.5940626/1‑94 (MQ=60)
         TCATTGCTCATGTCGTGAAGACTGATTTGCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGAT                                                                                      <  SRR4302545.1798019/94‑1 (MQ=60)
          CATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATG                                                                                     <  SRR4302545.3052726/94‑1 (MQ=60)
          CATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATG                                                                                     <  SRR4302545.1713392/94‑1 (MQ=60)
           ATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGT                                                                                    <  SRR4302545.7875779/94‑1 (MQ=60)
             TGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTG                                                                                  <  SRR4302545.3023585/94‑1 (MQ=60)
                TCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCA                                                                               <  SRR4302545.6771199/94‑1 (MQ=60)
                TCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCA                                                                               <  SRR4302545.6768135/94‑1 (MQ=60)
                TCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCA                                                                               <  SRR4302545.537576/94‑1 (MQ=60)
                TCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCA                                                                               >  SRR4302545.9432119/1‑94 (MQ=60)
                 CATGTCGTGAAGACTGATTTTCTGCCAGGCC‑CCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGG                                                                             >  SRR4302545.3547723/1‑94 (MQ=60)
                    GTCGTGAGGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCATCCTGATACGCCTGATAGGCTGCCGCGCGATGTTGCCCGGCG                                                                           <  SRR4302545.6857874/94‑1 (MQ=60)
                    GTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCG                                                                           <  SRR4302545.2586534/94‑1 (MQ=60)
                     TCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCACCCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGC                                                                          <  SRR4302545.6635003/94‑1 (MQ=60)
                     TCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGC                                                                          >  SRR4302545.583233/1‑94 (MQ=60)
                      CGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCT                                                                         >  SRR4302545.4059582/1‑94 (MQ=60)
                      CGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCT                                                                         >  SRR4302545.4059583/1‑94 (MQ=60)
                      CGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCT                                                                         >  SRR4302545.1127981/1‑94 (MQ=60)
                       GTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTC                                                                        >  SRR4302545.1766469/1‑94 (MQ=60)
                       GTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTC                                                                        <  SRR4302545.1047678/94‑1 (MQ=60)
                        TGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCG                                                                       >  SRR4302545.2446250/1‑94 (MQ=60)
                        TGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAATCCTCAACCTGATACGCCTGATAGGCTACCGCACGATGTTGCCAGGCGCTCG                                                                       >  SRR4302545.5661881/1‑94 (MQ=60)
                         GAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGG                                                                      >  SRR4302545.1699196/1‑94 (MQ=60)
                           AGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTT                                                                    >  SRR4302545.5780160/1‑94 (MQ=60)
                           AGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTAGGTT                                                                    >  SRR4302545.3559256/1‑94 (MQ=60)
                            GACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTG                                                                   >  SRR4302545.17749/1‑94 (MQ=60)
                            GACTGATTTTCTGCCAGGCCGCAAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTG                                                                   <  SRR4302545.10727156/94‑1 (MQ=60)
                              CTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTC                                                                 <  SRR4302545.988338/94‑1 (MQ=60)
                               TGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCG                                                                >  SRR4302545.4882236/1‑94 (MQ=60)
                               TGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCG                                                                <  SRR4302545.3437037/94‑1 (MQ=60)
                               TGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCG                                                                >  SRR4302545.333767/1‑94 (MQ=60)
                                TATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCATGCGCTCAGTTGTCGT                                                               <  SRR4302545.9305873/94‑1 (MQ=60)
                                GATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGT                                                               <  SRR4302545.9305874/94‑1 (MQ=60)
                                 ATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTT                                                              >  SRR4302545.2418827/1‑94 (MQ=60)
                                     TCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTC                                                          >  SRR4302545.9892433/1‑94 (MQ=60)
                                     TCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTC                                                          <  SRR4302545.6181710/94‑1 (MQ=60)
                                     TCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTC                                                          >  SRR4302545.583713/1‑94 (MQ=60)
                                     TCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCCCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTC                                                          <  SRR4302545.7068251/94‑1 (MQ=60)
                                      CTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCG                                                         >  SRR4302545.85198/1‑94 (MQ=60)
                                       TGCCAGGCCGCCAGTGCGGTGGCGTATTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCCGGCGCTCGGTTGTCGTCGTTCGG                                                        >  SRR4302545.6995485/1‑94 (MQ=60)
                                       TGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGG                                                        >  SRR4302545.6995484/1‑94 (MQ=60)
                                       TGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGG                                                        >  SRR4302545.4524965/1‑94 (MQ=60)
                                        GCCAGGCCGCCAGTGCGGTGGTGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGC                                                       >  SRR4302545.10491101/1‑94 (MQ=60)
                                        GCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGC                                                       <  SRR4302545.8496137/94‑1 (MQ=60)
                                         CCAGGTCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCC                                                      >  SRR4302545.1079054/1‑94 (MQ=60)
                                         CCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCC                                                      >  SRR4302545.4857379/1‑94 (MQ=60)
                                         CCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCC                                                      >  SRR4302545.4477317/1‑94 (MQ=60)
                                         CCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCC                                                      >  SRR4302545.9447819/1‑94 (MQ=60)
                                          CAGGCCGCCAGTGCGGTGGCGTAGTCCTCAGCCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCT                                                     <  SRR4302545.7517517/94‑1 (MQ=60)
                                          CAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCT                                                     <  SRR4302545.7517518/94‑1 (MQ=60)
                                           CGGCCGCCAGGGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTG                                                    <  SRR4302545.642855/94‑1 (MQ=60)
                                            GGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGA                                                   >  SRR4302545.9441489/1‑94 (MQ=60)
                                            GGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGA                                                   >  SRR4302545.954130/1‑94 (MQ=60)
                                               CGCCAGTGGGGTGGCGTAGTCCTCAACCTGAGACGCCTGATCGGCTGCCGCAAGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGC                                                >  SRR4302545.3132353/1‑94 (MQ=60)
                                               CGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGC                                                >  SRR4302545.2413055/1‑94 (MQ=60)
                                               CGCCAGTGCGGTGGCGTAGGCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGGTGTCGTTGTTCGGCCGGAAGC                                                >  SRR4302545.3132352/1‑94 (MQ=60)
                                                GCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCC                                               >  SRR4302545.3455169/1‑94 (MQ=60)
                                                 CCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCA                                              >  SRR4302545.6814995/1‑94 (MQ=60)
                                                   AGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATG                                            <  SRR4302545.10929895/94‑1 (MQ=60)
                                                    GTGCGGTGGCGTAGTCCTCAACCTGATACGCTTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGTTTGTCGTTGTTCGGCCTGAAGCCATGC                                           <  SRR4302545.1505652/94‑1 (MQ=60)
                                                    GTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGC                                           <  SRR4302545.3966981/94‑1 (MQ=60)
                                                     TGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCA                                          >  SRR4302545.3543436/1‑94 (MQ=60)
                                                      GCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGGTGCCAGGCGCTCGGTTGTCGTTGGTCGGCCCGAAGCCATGCAT                                         >  SRR4302545.9683095/1‑94 (MQ=60)
                                                       CGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATA                                        <  SRR4302545.8117925/94‑1 (MQ=60)
                                                       CGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATA                                        <  SRR4302545.7722936/94‑1 (MQ=60)
                                                       CGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATA                                        <  SRR4302545.356030/94‑1 (MQ=60)
                                                       CGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATA                                        <  SRR4302545.1581482/94‑1 (MQ=60)
                                                        GGTGGCGTAGTCCTCAACCTGATGCGCCTGATAGGCTGCCGCACGATGTTGTCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATAC                                       <  SRR4302545.7763522/94‑1 (MQ=60)
                                                         GTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACA                                      >  SRR4302545.9133832/1‑94 (MQ=60)
                                                          TGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAA                                     <  SRR4302545.10086549/94‑1 (MQ=60)
                                                           GGCGTAGTCCTCAACCTGATACGCCTGATAGGCTACCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAAC                                    >  SRR4302545.9300865/1‑94 (MQ=60)
                                                             CGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGC                                  <  SRR4302545.7015501/94‑1 (MQ=60)
                                                             CGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTGCGGCCTGAAGCCATGCATACAACGC                                  <  SRR4302545.5785261/94‑1 (MQ=60)
                                                             CGTAGTCCTCAACCTGATACGCCTGATAGGCTGACGCACGATGTTGCTAGGAGCTCCGTTATCGTTGTTCGGCCTGAATCCATGCATACAACGA                                  >  SRR4302545.6187990/1‑94 (MQ=60)
                                                              cttGTCCTCAACCTGATACGCCTGATAGGCTCCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCC                                 <  SRR4302545.1852129/91‑1 (MQ=60)
                                                               tagacctcaccctgagccgtCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTAGGTTGCCGTTGTTCGGACTGAACCCATGCATACAACGCCA                                <  SRR4302545.4425373/74‑1 (MQ=60)
                                                               TAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCGA                                >  SRR4302545.430221/1‑94 (MQ=60)
                                                               TAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCA                                >  SRR4302545.5118649/1‑94 (MQ=60)
                                                               TAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCA                                <  SRR4302545.8414325/94‑1 (MQ=60)
                                                                AGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCAC                               >  SRR4302545.6354272/1‑94 (MQ=60)
                                                                AGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCAA                               >  SRR4302545.2855825/1‑94 (MQ=60)
                                                                 GTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACA                              >  SRR4302545.3257812/1‑94 (MQ=60)
                                                                 GTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACA                              <  SRR4302545.7439605/94‑1 (MQ=60)
                                                                  TCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACAC                             >  SRR4302545.10385921/1‑94 (MQ=60)
                                                                   CCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCCGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACACAGCCACACC                            >  SRR4302545.153610/1‑94 (MQ=60)
                                                                   CCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACC                            >  SRR4302545.9105554/1‑94 (MQ=60)
                                                                   CCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACC                            <  SRR4302545.6459745/94‑1 (MQ=60)
                                                                    CTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCG                           >  SRR4302545.5609444/1‑94 (MQ=60)
                                                                     TCAACCTGATACGTCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGG                          <  SRR4302545.8238147/94‑1 (MQ=60)
                                                                      CAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGG                         <  SRR4302545.8540193/94‑1 (MQ=60)
                                                                      CAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGG                         <  SRR4302545.365920/94‑1 (MQ=60)
                                                                      CAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGG                         <  SRR4302545.10306535/94‑1 (MQ=60)
                                                                      CAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGG                         <  SRR4302545.7713539/94‑1 (MQ=60)
                                                                       AACCTGATACGCCTGCTAGGCTGCGGCACGATGTTGCCAGGCGCTCGTTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCCCACCGGGT                        <  SRR4302545.1097821/94‑1 (MQ=60)
                                                                       AACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGT                        >  SRR4302545.4092273/1‑94 (MQ=60)
                                                                       AACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGT                        >  SRR4302545.9412819/1‑94 (MQ=60)
                                                                       AACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGT                        <  SRR4302545.2592157/94‑1 (MQ=60)
                                                                       AACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGA                        >  SRR4302545.9412813/1‑94 (MQ=60)
                                                                        ACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTA                       <  SRR4302545.3625302/94‑1 (MQ=60)
                                                                        ACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTA                       <  SRR4302545.7135684/94‑1 (MQ=60)
                                                                        ACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTA                       <  SRR4302545.3994355/94‑1 (MQ=60)
                                                                        ACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTA                       <  SRR4302545.2647552/94‑1 (MQ=60)
                                                                           TGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCG                    >  SRR4302545.2761957/1‑94 (MQ=60)
                                                                           TGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCG                    <  SRR4302545.5056785/94‑1 (MQ=60)
                                                                           TGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCG                    >  SRR4302545.1403324/1‑94 (MQ=60)
                                                                            GATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATAAAACGCCACACCGGGTAGCGG                   >  SRR4302545.6412430/1‑94 (MQ=60)
                                                                             ATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTG                  >  SRR4302545.10743182/1‑94 (MQ=60)
                                                                             ATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTG                  <  SRR4302545.212667/94‑1 (MQ=60)
                                                                             ATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTG                  >  SRR4302545.7508608/1‑94 (MQ=60)
                                                                              TACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGT                 <  SRR4302545.4170071/94‑1 (MQ=60)
                                                                              TACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGT                 <  SRR4302545.874652/94‑1 (MQ=60)
                                                                                CGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCC               >  SRR4302545.4244096/1‑94 (MQ=60)
                                                                                 GCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCC              <  SRR4302545.4088179/94‑1 (MQ=60)
                                                                                 GCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCC              <  SRR4302545.4961647/94‑1 (MQ=60)
                                                                                  CCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCG             >  SRR4302545.5591862/1‑94 (MQ=60)
                                                                                  CCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCG             <  SRR4302545.3646008/94‑1 (MQ=60)
                                                                                  CCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCG             <  SRR4302545.234184/94‑1 (MQ=60)
                                                                                    TGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGAT           >  SRR4302545.2662808/1‑94 (MQ=60)
                                                                                     GATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATA          >  SRR4302545.2396508/1‑94 (MQ=60)
                                                                                       TAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAAC        <  SRR4302545.7883535/94‑1 (MQ=60)
                                                                                       TAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAAC        <  SRR4302545.1606771/94‑1 (MQ=60)
                                                                                        AGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACA       >  SRR4302545.3285900/1‑94 (MQ=60)
                                                                                        AGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACA       >  SRR4302545.5367550/1‑94 (MQ=60)
                                                                                        AGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACAACCGGTAGCGGGGCCCGATAACA       >  SRR4302545.9583425/1‑94 (MQ=60)
                                                                                        AGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGGTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACA       >  SRR4302545.5074095/1‑94 (MQ=60)
                                                                                           CTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTT    <  SRR4302545.7108606/94‑1 (MQ=60)
                                                                                           CTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTT    >  SRR4302545.1687033/1‑94 (MQ=60)
                                                                                           CTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTT    >  SRR4302545.3623199/1‑94 (MQ=60)
                                                                                            TGCCGCACGATGTTGCGAGGCGCTCGGTTGTCGTTGTTCGGGCTGAAGCCATGCAGACAACGCCACACCTGGTAGCGTGTCCCGATAACACTTT   <  SRR4302545.10585514/94‑1 (MQ=60)
                                                                                             GCCGCACGTTGTTGCCAGGCGCTCGGTTGTCGTAGTTCAGCCTGAAGCCATGCATACAACGCCACACAGGGTAGCGTGTCCCGATAACACTTTA  <  SRR4302545.9678803/94‑1 (MQ=60)
                                                                                             GCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTTTG  <  SRR4302545.2798990/94‑1 (MQ=60)
                                                                                             |                                                                                             
AGCAGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTACCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTTTG  >  NC_000913/588532‑588718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑