Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 588,625 A→G V518A (GTA→GCA)  nfrA ← NfrA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913588,6250AG100.0% 628.4 / NA 182V518A (GTA→GCA) nfrANfrA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (105/77);  total (105/77)

Only 100 of 189 total aligned reads displayed.

AGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTACCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACAC  >  NC_000913/588535‑588714
                                                                                          |                                                                                         
aGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGcc                                                                                         >  1:1288944/1‑93 (MQ=255)
aGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGcc                                                                                         >  2:1566147/1‑93 (MQ=255)
aGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGcc                                                                                         >  1:2622154/1‑93 (MQ=255)
 gATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCg                                                                                        >  2:2896451/1‑93 (MQ=255)
 gATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCg                                                                                        >  2:501494/1‑93 (MQ=255)
 gATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCg                                                                                        <  2:739464/93‑1 (MQ=255)
 gATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCg                                                                                        >  2:3782442/1‑93 (MQ=255)
 gATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCg                                                                                        >  2:6046716/1‑93 (MQ=255)
   tCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCa                                                                                      <  1:2798617/93‑1 (MQ=255)
   tCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCa                                                                                      >  1:3183919/1‑93 (MQ=255)
   tCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCa                                                                                      >  2:4858106/1‑93 (MQ=255)
     cTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACg                                                                                    >  2:3435912/1‑93 (MQ=255)
      tCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGa                                                                                   <  2:1135036/93‑1 (MQ=255)
        aTTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATg                                                                                 >  1:3814962/1‑93 (MQ=255)
        aTTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATg                                                                                 >  2:1798729/1‑93 (MQ=255)
         ttGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGt                                                                                >  1:5557275/1‑93 (MQ=255)
           gCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTg                                                                              >  2:4167734/1‑93 (MQ=255)
           gCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTg                                                                              >  1:3638336/1‑93 (MQ=255)
           gCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTg                                                                              >  1:2215523/1‑93 (MQ=255)
            cTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGc                                                                             >  1:2743226/1‑93 (MQ=255)
            cTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGc                                                                             <  2:4322545/93‑1 (MQ=255)
            cTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGc                                                                             <  2:3633217/93‑1 (MQ=255)
             tCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGcc                                                                            <  2:1310221/93‑1 (MQ=255)
             tCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGcc                                                                            >  2:1208255/1‑93 (MQ=255)
              cATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCa                                                                           <  1:1769725/93‑1 (MQ=255)
              cATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCa                                                                           <  1:3243196/93‑1 (MQ=255)
               aTGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAg                                                                          <  1:4521698/93‑1 (MQ=255)
               aTGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAg                                                                          >  1:1905114/1‑93 (MQ=255)
                  tCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAggag                                                                       >  2:3182172/1‑91 (MQ=255)
                  tCGTGAAGACTGAGTTTCTGCTAGGCCTCAAGTGCGGTGGCGTAGTCCTCACCCTGATACGCGTGATAGGCTGCCGCACGATGTTGCCAGgcg                                                                       <  2:470288/93‑1 (MQ=255)
                    gTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCt                                                                     >  2:5468276/1‑93 (MQ=255)
                     tGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTc                                                                    >  1:494014/1‑93 (MQ=255)
                        aGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGt                                                                 <  2:4947679/93‑1 (MQ=255)
                         gACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGtt                                                                <  2:5890391/93‑1 (MQ=255)
                         gACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGtt                                                                >  1:4158073/1‑93 (MQ=255)
                          aCTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTg                                                               <  2:2565229/93‑1 (MQ=255)
                          aCTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTg                                                               <  1:1399594/93‑1 (MQ=255)
                          aCTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTg                                                               >  1:5148838/1‑93 (MQ=255)
                            tGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTc                                                             >  1:3443802/1‑93 (MQ=255)
                            tGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTc                                                             >  2:1055924/1‑93 (MQ=255)
                               ttttCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCgtt                                                          >  2:1695676/1‑93 (MQ=255)
                               ttttCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCgtt                                                          >  2:732218/1‑93 (MQ=255)
                                tttCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCgttg                                                         <  1:52096/93‑1 (MQ=255)
                                  tCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCgttgtt                                                       <  1:2358511/93‑1 (MQ=255)
                                   cTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTc                                                      <  1:5769991/93‑1 (MQ=255)
                                    tGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCg                                                     <  2:2935785/93‑1 (MQ=255)
                                      ccAGGCGGCCAGTGCGGTGGCGTAGTCCTCAGCCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGc                                                   <  2:302616/93‑1 (MQ=255)
                                      ccAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGc                                                   >  1:6215004/1‑93 (MQ=255)
                                      ccAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGc                                                   >  2:2796514/1‑93 (MQ=255)
                                       cAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGcc                                                  >  1:249316/1‑93 (MQ=255)
                                          gccgccAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGa                                               <  1:6010242/93‑1 (MQ=255)
                                          gccgccAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGa                                               >  2:3872614/1‑93 (MQ=255)
                                            cgccAGTGCGGTGGCGTAGTCGTCAACCTGAGACGCATGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAg                                             <  2:5996407/93‑1 (MQ=255)
                                             gccAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGc                                            <  1:498226/93‑1 (MQ=255)
                                             gccAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGc                                            >  2:4513349/1‑93 (MQ=255)
                                             gccAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGc                                            >  2:4678304/1‑93 (MQ=255)
                                             gccAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGc                                            >  1:3729623/1‑93 (MQ=255)
                                              ccAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGcc                                           <  2:14792/93‑1 (MQ=255)
                                              ccAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGcc                                           >  1:943806/1‑93 (MQ=255)
                                              ccAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGcc                                           >  1:5622043/1‑93 (MQ=255)
                                              ccAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGcc                                           >  2:4756412/1‑93 (MQ=255)
                                               cAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCa                                          <  2:6342089/93‑1 (MQ=255)
                                                aGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCAt                                         <  1:5189330/93‑1 (MQ=255)
                                                aGTGCGCTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCAt                                         <  2:2689686/93‑1 (MQ=255)
                                                 gTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATg                                        >  2:1930668/1‑93 (MQ=255)
                                                  tGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGc                                       <  1:1027152/93‑1 (MQ=255)
                                                   gCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCa                                      >  1:1450593/1‑93 (MQ=255)
                                                    cGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCAt                                     <  2:4411187/93‑1 (MQ=255)
                                                     ggTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATa                                    <  2:2758400/93‑1 (MQ=255)
                                                      gTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATAc                                   >  1:1078178/1‑93 (MQ=255)
                                                       gggCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACa                                  <  2:3215670/92‑1 (MQ=255)
                                                        ggCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACaa                                 >  2:2441984/1‑93 (MQ=255)
                                                         gCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAAc                                <  1:1746561/93‑1 (MQ=255)
                                                          cGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACg                               >  1:5262585/1‑93 (MQ=255)
                                                           gTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGc                              <  2:2533783/93‑1 (MQ=255)
                                                            tAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGcc                             >  2:1148243/1‑93 (MQ=255)
                                                               tCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCcaca                          >  1:2010432/1‑93 (MQ=255)
                                                               tCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCcaca                          >  1:197881/1‑93 (MQ=255)
                                                               tCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCcaca                          <  1:1862056/93‑1 (MQ=255)
                                                                 cTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACAcc                        >  1:6439113/1‑93 (MQ=255)
                                                                  tCAACCTGATACGCCTGATATGCTGCCGCACGATGTTGCCAGGCGCTCGGGTGTCGTTGTTCGGTCCGAAGCCATGCATACAACGCCACACCg                       >  2:5587342/1‑93 (MQ=255)
                                                                   cAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCgg                      <  2:2730739/93‑1 (MQ=255)
                                                                   cAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCgg                      <  1:81647/93‑1 (MQ=255)
                                                                   cAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCgg                      <  2:1629306/93‑1 (MQ=255)
                                                                    aaCCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCggg                     >  1:3398453/1‑93 (MQ=255)
                                                                      ccTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTa                   <  2:4200627/93‑1 (MQ=255)
                                                                      ccTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTa                   <  2:2183283/93‑1 (MQ=255)
                                                                      ccTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTa                   <  1:270518/93‑1 (MQ=255)
                                                                         gATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCg                >  2:1827187/1‑93 (MQ=255)
                                                                           tACGCCTGATAGGCTGCCGCACGATTTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCgtg              >  1:2792497/1‑93 (MQ=255)
                                                                               ccTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTccc          <  1:723888/93‑1 (MQ=255)
                                                                               ccTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTccc          <  1:4565863/93‑1 (MQ=255)
                                                                               ccTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTccc          >  1:3298513/1‑93 (MQ=255)
                                                                                 tGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGa        >  1:3046946/1‑93 (MQ=255)
                                                                                 tGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCAACACCGGGTAGGGTGTCCCGa        >  2:833555/1‑93 (MQ=255)
                                                                                  gATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGAt       >  1:442401/1‑93 (MQ=255)
                                                                                    tAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATaa     <  2:544603/93‑1 (MQ=255)
                                                                                      ggCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAaca   >  1:2917342/1‑93 (MQ=255)
                                                                                       gCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAacac  >  1:208031/1‑93 (MQ=255)
                                                                                       gCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAacac  <  2:4304810/93‑1 (MQ=255)
                                                                                          |                                                                                         
AGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTACCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACAC  >  NC_000913/588535‑588714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCAGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTACCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTTTG  >  NC_000913/588532‑588718
                                                                                             |                                                                                             
AGCAGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTG                                                                                               <  SRR4302537.5654366/94‑1 (MQ=60)
AGCAGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTG                                                                                               <  SRR4302537.6349965/94‑1 (MQ=60)
 GCAGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGC                                                                                              <  SRR4302537.55912/94‑1 (MQ=60)
 GCAGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGC                                                                                              <  SRR4302537.3354746/94‑1 (MQ=60)
  CAGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCTCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCC                                                                                             <  SRR4302537.4372159/94‑1 (MQ=60)
  CAGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCC                                                                                             >  SRR4302537.2640528/1‑94 (MQ=60)
  CAGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCC                                                                                             >  SRR4302537.5240580/1‑94 (MQ=60)
  CAGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCC                                                                                             >  SRR4302537.1297911/1‑94 (MQ=60)
   AGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCG                                                                                            <  SRR4302537.3118957/94‑1 (MQ=60)
   AGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCG                                                                                            >  SRR4302537.1764874/1‑94 (MQ=60)
   AGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCATGATAGGCTGCCG                                                                                            >  SRR4302537.191303/1‑94 (MQ=60)
    GATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGC                                                                                           >  SRR4302537.6376680/1‑94 (MQ=60)
     ATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCA                                                                                          >  SRR4302537.1409686/1‑94 (MQ=60)
     ATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCA                                                                                          >  SRR4302537.3206298/1‑94 (MQ=60)
      TCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCAC                                                                                         <  SRR4302537.2818183/94‑1 (MQ=60)
        CTCATTGCTCATGTCGTGAAGACTGATTTTCTTCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGA                                                                                       >  SRR4302537.1949086/1‑94 (MQ=60)
         TCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGAT                                                                                      >  SRR4302537.862661/1‑94 (MQ=60)
         TCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGATGCCGCACGAT                                                                                      >  SRR4302537.2753994/1‑94 (MQ=60)
          CATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATG                                                                                     <  SRR4302537.2964690/94‑1 (MQ=60)
          CATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATG                                                                                     >  SRR4302537.3841713/1‑94 (MQ=60)
           ATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGT                                                                                    >  SRR4302537.5595246/1‑94 (MQ=60)
           ATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGT                                                                                    >  SRR4302537.2695647/1‑94 (MQ=60)
            TTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTT                                                                                   <  SRR4302537.45802/94‑1 (MQ=60)
             TGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTG                                                                                  >  SRR4302537.2231145/1‑94 (MQ=60)
             TGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTG                                                                                  >  SRR4302537.3663953/1‑94 (MQ=60)
              TCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGC                                                                                 <  SRR4302537.4008446/94‑1 (MQ=60)
              GCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGC                                                                                 >  SRR4302537.2762362/1‑94 (MQ=60)
               CTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCC                                                                                <  SRR4302537.5048299/94‑1 (MQ=60)
                 CATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAG                                                                              <  SRR4302537.3266055/94‑1 (MQ=60)
                 CATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAG                                                                              <  SRR4302537.1782049/94‑1 (MQ=60)
                 CATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAG                                                                              >  SRR4302537.1918344/1‑94 (MQ=60)
                  ATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGG                                                                             >  SRR4302537.2378738/1‑94 (MQ=60)
                  ATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGG                                                                             <  SRR4302537.4552978/94‑1 (MQ=60)
                    GTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCG                                                                           >  SRR4302537.3764812/1‑94 (MQ=60)
                       GTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTC                                                                        >  SRR4302537.497417/1‑94 (MQ=60)
                       GTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTC                                                                        >  SRR4302537.3229985/1‑94 (MQ=60)
                         GAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGG                                                                      >  SRR4302537.3555754/1‑94 (MQ=60)
                           AGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTT                                                                    >  SRR4302537.4187112/1‑94 (MQ=60)
                           AGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTT                                                                    >  SRR4302537.588572/1‑94 (MQ=60)
                            GACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTG                                                                   >  SRR4302537.5184320/1‑94 (MQ=60)
                             ACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGT                                                                  <  SRR4302537.1409351/94‑1 (MQ=60)
                              CTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTC                                                                 >  SRR4302537.3468166/1‑94 (MQ=60)
                                   TTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGT                                                            <  SRR4302537.52484/94‑1 (MQ=60)
                                    TTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTT                                                           >  SRR4302537.2086420/1‑94 (MQ=60)
                                     TCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTC                                                          <  SRR4302537.2375150/94‑1 (MQ=60)
                                      CTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCG                                                         <  SRR4302537.5809540/94‑1 (MQ=60)
                                      CTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCG                                                         >  SRR4302537.3310714/1‑94 (MQ=60)
                                       TGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGG                                                        <  SRR4302537.1313525/94‑1 (MQ=60)
                                        GCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGC                                                       >  SRR4302537.6257801/1‑94 (MQ=60)
                                         CCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCC                                                      >  SRR4302537.251088/1‑94 (MQ=60)
                                           AGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTG                                                    <  SRR4302537.5377313/94‑1 (MQ=60)
                                           AGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTG                                                    <  SRR4302537.334201/94‑1 (MQ=60)
                                            GGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTCCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGA                                                   <  SRR4302537.2875365/94‑1 (MQ=60)
                                             GCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAA                                                  <  SRR4302537.6051534/94‑1 (MQ=60)
                                             GCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAA                                                  >  SRR4302537.95981/1‑94 (MQ=60)
                                               CGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGC                                                >  SRR4302537.3755824/1‑94 (MQ=60)
                                                GCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCC                                               <  SRR4302537.501651/94‑1 (MQ=60)
                                                GCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCC                                               >  SRR4302537.950326/1‑94 (MQ=60)
                                                GCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCC                                               >  SRR4302537.5660480/1‑94 (MQ=60)
                                                  CAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCAT                                             <  SRR4302537.4986183/94‑1 (MQ=60)
                                                  CAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCAT                                             >  SRR4302537.5755485/1‑94 (MQ=60)
                                                   AGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGGCTGAAGCCATG                                            >  SRR4302537.1191494/1‑94 (MQ=60)
                                                   AGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATG                                            <  SRR4302537.5225021/94‑1 (MQ=60)
                                                     TGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCA                                          >  SRR4302537.1460700/1‑94 (MQ=60)
                                                     TGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCA                                          <  SRR4302537.1034222/94‑1 (MQ=60)
                                                        GGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATAC                                       >  SRR4302537.5247429/1‑94 (MQ=60)
                                                        GGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATAC                                       >  SRR4302537.1085615/1‑94 (MQ=60)
                                                        GGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCAAGCATAC                                       >  SRR4302537.228237/1‑94 (MQ=60)
                                                         GTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACA                                      >  SRR4302537.1619798/1‑94 (MQ=60)
                                                            GCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACG                                   <  SRR4302537.4459688/94‑1 (MQ=60)
                                                            GCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACG                                   <  SRR4302537.1758741/94‑1 (MQ=60)
                                                            GCGTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACG                                   >  SRR4302537.5298794/1‑94 (MQ=60)
                                                            GCGTAGGCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACG                                   <  SRR4302537.1816578/94‑1 (MQ=60)
                                                              GTAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCC                                 <  SRR4302537.5610184/94‑1 (MQ=60)
                                                               TAGTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCA                                >  SRR4302537.2190066/1‑94 (MQ=60)
                                                                 GTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACA                              >  SRR4302537.2024444/1‑94 (MQ=60)
                                                                 GTCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACA                              >  SRR4302537.199293/1‑94 (MQ=60)
                                                                  TCCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACAC                             <  SRR4302537.1875024/94‑1 (MQ=60)
                                                                   CCTCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACC                            >  SRR4302537.6482823/1‑94 (MQ=60)
                                                                     TCAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGG                          <  SRR4302537.814861/94‑1 (MQ=60)
                                                                      CAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGG                         >  SRR4302537.3422526/1‑94 (MQ=60)
                                                                      CAACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGG                         <  SRR4302537.82247/94‑1 (MQ=60)
                                                                        ACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTA                       <  SRR4302537.2591872/94‑1 (MQ=60)
                                                                        ACCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTA                       >  SRR4302537.4892174/1‑94 (MQ=60)
                                                                         CCTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAG                      <  SRR4302537.272423/94‑1 (MQ=60)
                                                                          CTGATACGCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGC                     >  SRR4302537.3435116/1‑94 (MQ=60)
                                                                             ATACGCCTGATAGGCTGCCGCACGATTTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTG                  >  SRR4302537.2812013/1‑94 (MQ=60)
                                                                                 GCCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCC              >  SRR4302537.3321834/1‑94 (MQ=60)
                                                                                  CCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCG             <  SRR4302537.4597468/94‑1 (MQ=60)
                                                                                  CCTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCG             <  SRR4302537.728830/94‑1 (MQ=60)
                                                                                   CTGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGA            >  SRR4302537.3068174/1‑94 (MQ=60)
                                                                                    TGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGAT           >  SRR4302537.445475/1‑94 (MQ=60)
                                                                                    TGATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGAT           >  SRR4302537.6196666/1‑94 (MQ=60)
                                                                                      ATAGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAA         >  SRR4302537.4217617/1‑94 (MQ=60)
                                                                                        AGGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACA       >  SRR4302537.2937837/1‑94 (MQ=60)
                                                                                         GGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACAC      >  SRR4302537.209527/1‑94 (MQ=60)
                                                                                         GGCTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACAC      >  SRR4302537.2800746/1‑94 (MQ=60)
                                                                                           CTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTT    <  SRR4302537.957366/94‑1 (MQ=60)
                                                                                           CTGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTT    <  SRR4302537.4685517/94‑1 (MQ=60)
                                                                                            TGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTTT   <  SRR4302537.5243158/94‑1 (MQ=60)
                                                                                            TGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTTT   <  SRR4302537.4772624/94‑1 (MQ=60)
                                                                                            TGCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTTT   >  SRR4302537.4199231/1‑94 (MQ=60)
                                                                                             GCCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTTTG  <  SRR4302537.1221787/94‑1 (MQ=60)
                                                                                             |                                                                                             
AGCAGATCCTCATTGCTCATGTCGTGAAGACTGATTTTCTGCCAGGCCGCCAGTGCGGTGGCGTAGTCCTCAACCTGATACGCCTGATAGGCTACCGCACGATGTTGCCAGGCGCTCGGTTGTCGTTGTTCGGCCTGAAGCCATGCATACAACGCCACACCGGGTAGCGTGTCCCGATAACACTTTG  >  NC_000913/588532‑588718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑