Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	647	270.1	21258240	98.4%	20918108	94.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	976,048	A→G	Q167R (CAG→CGG)	mukB →	MukB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	976,048	0	A	G	97.6%	819.2 / 6.3	249	Q167R (CAG→CGG)	mukB	MukB
Reads supporting (aligned to +/- strand): ref base A (3/3); new base G (136/107); total (139/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.61e-01

Only 100 of 251 total aligned reads displayed.

ACCGAAACCCTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACAGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGACCGTAGCAAATT  >  NC_000913/975957‑976138

aCCGAAACCCTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGt                                                                                          >  1:10250692/1‑94 (MQ=255)

aCCGAAACCCTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGt                                                                                          <  2:3218030/94‑1 (MQ=255)

aCCGAAACCCTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGt                                                                                          <  1:4907170/94‑1 (MQ=255)

 ccGAAACCCTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGtt                                                                                         >  1:7550606/1‑94 (MQ=255)

 ccGAAACCCTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGtt                                                                                         >  2:5903349/1‑94 (MQ=255)

  cGAAACCCTGAACGACCGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTc                                                                                        <  1:6787376/94‑1 (MQ=255)

   gAAACCCTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCa                                                                                       >  1:5701763/1‑94 (MQ=255)

    aaaCCCTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTcca                                                                                      >  1:4723766/1‑92 (MQ=255)

    aaaCCCTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCaa                                                                                      <  2:2230807/94‑1 (MQ=255)

    aaaCCCTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCaa                                                                                      >  1:1118963/1‑94 (MQ=255)

    aaaCCCTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGCTTAAACGGTTCaa                                                                                      >  2:10551205/1‑94 (MQ=255)

        ccTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTcc                                                                                  <  2:5875365/94‑1 (MQ=255)

        ccTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTcc                                                                                  <  2:3481681/94‑1 (MQ=255)

         cTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCa                                                                                 >  2:8931888/1‑94 (MQ=255)

          tGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCAt                                                                                <  2:7194228/94‑1 (MQ=255)

          tGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCAt                                                                                <  2:6927555/94‑1 (MQ=255)

          tGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCAt                                                                                <  2:4586267/94‑1 (MQ=255)

           gaacgaacGCCGGGCGCGCGTGCTGCGGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCAtt                                                                               <  2:9012775/94‑1 (MQ=255)

           gaacgaacGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCAtt                                                                               <  1:3817440/94‑1 (MQ=255)

            aacgaacgCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTa                                                                              <  2:8294913/94‑1 (MQ=255)

             acgaacgCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTAc                                                                             <  2:5413790/94‑1 (MQ=255)

              cgaacgCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACt                                                                            <  2:2481436/94‑1 (MQ=255)

               gaacgCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTg                                                                           <  1:4151449/94‑1 (MQ=255)

               caacgCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTg                                                                           <  1:8893510/93‑1 (MQ=255)

                aacgCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGa                                                                          >  1:9982812/1‑94 (MQ=255)

                 acgCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGAt                                                                         >  1:1573607/1‑94 (MQ=255)

                 acgCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGAt                                                                         >  2:6209720/1‑94 (MQ=255)

                 acgCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGAt                                                                         >  1:1745284/1‑94 (MQ=255)

                  cgCCATGCGCGCGTGCTTCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGAtt                                                                        <  1:1152206/94‑1 (MQ=255)

                     cAGGCGCGCGTGCTGCCGCTTAACGAGCTTAAAGACAAGCGCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTAcc                                                                     <  2:2282929/94‑1 (MQ=255)

                     cAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTAcc                                                                     >  2:2111155/1‑94 (MQ=255)

                     cAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTAcc                                                                     >  2:4465141/1‑94 (MQ=255)

                     cAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTAcc                                                                     >  2:9261653/1‑94 (MQ=255)

                      aGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCa                                                                    >  2:8891464/1‑94 (MQ=255)

                      aGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCa                                                                    >  1:8285194/1‑94 (MQ=255)

                      aGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCa                                                                    >  1:2400387/1‑94 (MQ=255)

                       ggCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCAc                                                                   >  1:5948696/1‑94 (MQ=255)

                         cgcgcgTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTc                                                                 >  1:2944240/1‑94 (MQ=255)

                         cgcgcgTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTc                                                                 >  2:10272487/1‑94 (MQ=255)

                          gcgcgTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCg                                                                >  2:4195403/1‑94 (MQ=255)

                             cgTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTg                                                             <  1:8066910/94‑1 (MQ=255)

                             cgTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTg                                                             >  1:9545818/1‑94 (MQ=255)

                              gTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGa                                                            >  2:8339613/1‑94 (MQ=255)

                                gctgcCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATg                                                          <  1:7538233/94‑1 (MQ=255)

                                gctgcCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATg                                                          <  2:8625906/94‑1 (MQ=255)

                                 ctgcCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGt                                                         <  2:7883217/94‑1 (MQ=255)

                                  tgcCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGtt                                                        >  2:8145526/1‑94 (MQ=255)

                                  tgcCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGtt                                                        <  1:3460270/94‑1 (MQ=255)

                                   gcCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTc                                                       <  2:4766642/94‑1 (MQ=255)

                                    ccGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCg                                                      >  1:6913012/1‑94 (MQ=255)

                                    ccGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGGTCAACTCCATTACTGATTACCACTCGCTGATGTTCg                                                      >  1:10290884/1‑94 (MQ=255)

                                      gCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGAt                                                    >  1:4448094/1‑94 (MQ=255)

                                       cTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATc                                                   >  1:6286697/1‑94 (MQ=255)

                                        ttAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCt                                                  <  2:3745362/94‑1 (MQ=255)

                                        ttAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCt                                                  >  2:612059/1‑94 (MQ=255)

                                         tAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTg                                                 >  1:758048/1‑94 (MQ=255)

                                         tAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTg                                                 <  2:5193767/94‑1 (MQ=255)

                                         tAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTg                                                 <  2:5555557/94‑1 (MQ=255)

                                         tAACGAACTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGAACTg                                                 <  1:9383704/94‑1 (MQ=255)

                                          aaCGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTgg                                                <  1:9242620/94‑1 (MQ=255)

                                           aCGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTggg                                               <  2:4988915/94‑1 (MQ=255)

                                             gAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGca                                             >  1:391855/1‑94 (MQ=255)

                                             gAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGca                                             <  1:6368960/94‑1 (MQ=255)

                                             gAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGca                                             <  2:6490323/94‑1 (MQ=255)

                                              aGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGcat                                            >  1:6759451/1‑94 (MQ=255)

                                              aGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGcat                                            <  1:7400196/94‑1 (MQ=255)

                                              aGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGcat                                            >  1:9097489/1‑94 (MQ=255)

                                                cTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGcatca                                          <  2:3519563/94‑1 (MQ=255)

                                                 tGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGcatcat                                         >  1:1914760/1‑94 (MQ=255)

                                                 tGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGcatcat                                         >  1:2294464/1‑94 (MQ=255)

                                                        cAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCGCTCGCTGATGTTCGATCTGGGCATCATAGCGcgt                                  >  2:6526314/1‑94 (MQ=255)

                                                        cAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGcgt                                  >  2:7694981/1‑94 (MQ=255)

                                                         aaGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGcgtc                                 >  1:7923759/1‑94 (MQ=255)

                                                            cTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCgtcgtc                              <  1:9322221/94‑1 (MQ=255)

                                                             tagAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCTCTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCt                             <  1:5829984/92‑1 (MQ=255)

                                                               gAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTgc                           >  1:2155769/1‑94 (MQ=255)

                                                                aGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTgcg                          >  2:1991303/1‑94 (MQ=255)

                                                                   cGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTc                       >  1:8317518/1‑94 (MQ=255)

                                                                    gATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATTATCGCGCGCCGTCTGCGCttc                      >  1:6951588/1‑92 (MQ=255)

                                                                    gATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTcc                      >  2:8635789/1‑94 (MQ=255)

                                                                    gATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTcc                      <  2:1551704/94‑1 (MQ=255)

                                                                     aTGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCg                     >  1:5002093/1‑94 (MQ=255)

                                                                      tGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGCCATCATCGCGCGTCGTCTGCGCTCCGc                    >  2:3071699/1‑94 (MQ=255)

                                                                       ggAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCa                   <  2:7347916/94‑1 (MQ=255)

                                                                        gAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCAt                  >  1:7173763/1‑94 (MQ=255)

                                                                        gAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTA‑CACTCGCTGATGTTTGATCTGGGCATCATTGCGCGGCGTCTGCGCTCCGCCTc                 >  2:6890000/1‑94 (MQ=255)

                                                                         aaGGCGTGCAGTTTAAACAGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTACGCATc                 >  2:4376113/1‑94 (MQ=255)

                                                                          aGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCt                >  2:2770604/1‑94 (MQ=255)

                                                                          aGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCt                <  1:10506443/94‑1 (MQ=255)

                                                                           ggCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTg               <  2:1594806/94‑1 (MQ=255)

                                                                             cGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAc             >  1:8797149/1‑94 (MQ=255)

                                                                             cGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAc             >  2:1222711/1‑94 (MQ=255)

                                                                              gTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAcc            <  1:4625388/94‑1 (MQ=255)

                                                                              gTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAcc            >  1:8386320/1‑94 (MQ=255)

                                                                              gTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAcc            <  1:5587258/94‑1 (MQ=255)

                                                                              gTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAcc            >  1:10015769/1‑94 (MQ=255)

                                                                                 cAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGACCGTa         >  2:26186/1‑94 (MQ=255)

                                                                                  aGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGACCGTAg        >  2:3575591/1‑94 (MQ=255)

                                                                                    tttAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGACCGTAGCa      >  2:4304705/1‑94 (MQ=255)

                                                                                       aaaCGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGCCGCCTGTGCTCCGCATCTGACCGTAGAAAAt   >  2:2823565/1‑94 (MQ=255)

ACCGAAACCCTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACAGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGACCGTAGCAAATT  >  NC_000913/975957‑976138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACCGAAACCCTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACAGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGACCGTAGCAAATTCT  >  NC_000913/975957‑976140

ACCGAAACCCTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGT                                                                                            >  SRR4302545.10553267/1‑94 (MQ=60)

ACCGAAACCCTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGT                                                                                            <  SRR4302545.5031718/94‑1 (MQ=60)

ACCGAAACCCTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGT                                                                                            <  SRR4302545.6628956/94‑1 (MQ=60)

 CCGAAACCCTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTT                                                                                           >  SRR4302545.7764065/1‑94 (MQ=60)

  CGAAACCCTGAACGACCGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTC                                                                                          <  SRR4302545.6973648/94‑1 (MQ=60)

   GAAACCCTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCA                                                                                         <  SRR4302545.4032167/94‑1 (MQ=60)

   GAAACCCTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCA                                                                                         >  SRR4302545.5854468/1‑94 (MQ=60)

    AAACCCTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCCA                                                                                        >  SRR4302545.4844217/1‑94 (MQ=60)

    AAACCCTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAA                                                                                        >  SRR4302545.1143366/1‑94 (MQ=60)

      ACCCTGAACGAACGCCAGGCTCGCTTGCTGCAGCTTAACGAGCTGAAAGACAAGCGCGAGGCGATGGAAGGCTTGCAGTTTAAACGGTTCAACT                                                                                      <  SRR4302545.4524887/94‑1 (MQ=60)

         CTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCA                                                                                   >  SRR4302545.7023953/1‑94 (MQ=60)

          TGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCAT                                                                                  >  SRR4302545.966222/1‑94 (MQ=60)

          TGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCACGAGGCGATGGAAGGCGTGCAGCTTAAACGGTTCAACTCCAT                                                                                  >  SRR4302545.9620315/1‑94 (MQ=60)

           GAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATT                                                                                 <  SRR4302545.3912392/94‑1 (MQ=60)

           GAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATT                                                                                 >  SRR4302545.7759481/1‑94 (MQ=60)

            AACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTA                                                                                >  SRR4302545.8460185/1‑94 (MQ=60)

              CGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACT                                                                              >  SRR4302545.2434268/1‑94 (MQ=60)

              CGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACT                                                                              >  SRR4302545.3048787/1‑94 (MQ=60)

              CGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACT                                                                              >  SRR4302545.9348/1‑94 (MQ=60)

              CGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACT                                                                              >  SRR4302545.1144688/1‑94 (MQ=60)

               GAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTG                                                                             >  SRR4302545.711833/1‑94 (MQ=60)

               GAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTG                                                                             <  SRR4302545.4255120/94‑1 (MQ=60)

               CAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTG                                                                             <  SRR4302545.9151992/94‑1 (MQ=60)

                AACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGA                                                                            >  SRR4302545.10278874/1‑94 (MQ=60)

                AACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGA                                                                            >  SRR4302545.2744163/1‑94 (MQ=60)

                 ACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGAT                                                                           >  SRR4302545.1610714/1‑94 (MQ=60)

                 ACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGAT                                                                           >  SRR4302545.1787039/1‑94 (MQ=60)

                  CGCCATGCGCGCGTGCTTCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATT                                                                          <  SRR4302545.1177484/94‑1 (MQ=60)

                      GGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCA                                                                      <  SRR4302545.1825422/94‑1 (MQ=60)

                      AGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCA                                                                      >  SRR4302545.8525189/1‑94 (MQ=60)

                      AGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCA                                                                      >  SRR4302545.2458603/1‑94 (MQ=60)

                       GGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCAC                                                                     >  SRR4302545.6109191/1‑94 (MQ=60)

                        GCGCGCGTGCGGCCACTTAACGAGCTGAAAGACAAGCGCGAGGCGATGGAAGGCGTTCAGTTTAAACGGTTCAACTCCATTACTGATTACCACT                                                                    <  SRR4302545.8531979/94‑1 (MQ=60)

                         CGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTC                                                                   >  SRR4302545.3015871/1‑94 (MQ=60)

                         CGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTC                                                                   >  SRR4302545.5085288/1‑94 (MQ=60)

                          GCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCG                                                                  <  SRR4302545.3828686/94‑1 (MQ=60)

                             CGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTG                                                               >  SRR4302545.9827040/1‑94 (MQ=60)

                             CGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTG                                                               <  SRR4302545.8297857/94‑1 (MQ=60)

                                GCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATG                                                            <  SRR4302545.7751441/94‑1 (MQ=60)

                                 CTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGT                                                           >  SRR4302545.7948552/1‑94 (MQ=60)

                                  TGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTT                                                          <  SRR4302545.3544975/94‑1 (MQ=60)

                                    CCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCG                                                        >  SRR4302545.10594360/1‑94 (MQ=60)

                                    CCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCG                                                        >  SRR4302545.7103484/1‑94 (MQ=60)

                                    CCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGGTCAACTCCATTACTGATTACCACTCGCTGATGTTCG                                                        >  SRR4302545.10594361/1‑94 (MQ=60)

                                      GCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGAT                                                      <  SRR4302545.7789058/94‑1 (MQ=60)

                                      GCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGAT                                                      <  SRR4302545.7789059/94‑1 (MQ=60)

                                      GCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGAT                                                      >  SRR4302545.4561442/1‑94 (MQ=60)

                                       CTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATC                                                     >  SRR4302545.6458104/1‑94 (MQ=60)

                                        TTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCT                                                    >  SRR4302545.5014117/1‑94 (MQ=60)

                                         TAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTG                                                   >  SRR4302545.775101/1‑94 (MQ=60)

                                         TAACGAACTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGAACTG                                                   <  SRR4302545.9659099/94‑1 (MQ=60)

                                          agcgacgTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTGCCCCTCGCTGATGTTCGATCTGG                                                  <  SRR4302545.6046041/87‑1 (MQ=60)

                                          AACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGG                                                  <  SRR4302545.8679504/94‑1 (MQ=60)

                                          AACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGG                                                  <  SRR4302545.394889/94‑1 (MQ=60)

                                          AACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGG                                                  <  SRR4302545.9513611/94‑1 (MQ=60)

                                            CGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGC                                                >  SRR4302545.7028694/1‑94 (MQ=60)

                                             GAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGGA                                               >  SRR4302545.8533816/1‑94 (MQ=60)

                                             GAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCA                                               >  SRR4302545.400582/1‑94 (MQ=60)

                                             GAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCA                                               <  SRR4302545.6543042/94‑1 (MQ=60)

                                             GAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGGTCGATCTGGGCA                                               >  SRR4302545.8533817/1‑94 (MQ=60)

                                              AGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCAT                                              <  SRR4302545.9813923/94‑1 (MQ=60)

                                              AGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCAT                                              <  SRR4302545.7609152/94‑1 (MQ=60)

                                              AGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCAT                                              >  SRR4302545.6944798/1‑94 (MQ=60)

                                              AGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCAT                                              >  SRR4302545.6944797/1‑94 (MQ=60)

                                              AGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCAT                                              >  SRR4302545.9364009/1‑94 (MQ=60)

                                                CTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCA                                            <  SRR4302545.6003663/94‑1 (MQ=60)

                                                 TGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCAT                                           >  SRR4302545.3711987/1‑94 (MQ=60)

                                                 TGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCAT                                           >  SRR4302545.2350338/1‑94 (MQ=60)

                                                 TGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCAT                                           >  SRR4302545.1962087/1‑94 (MQ=60)

                                                  GAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGCTTACCACTCGCTGATGTTCGATCTGGGCATCATC                                          >  SRR4302545.2332659/1‑94 (MQ=60)

                                                  GAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATC                                          <  SRR4302545.10784777/94‑1 (MQ=60)

                                                  GAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATC                                          <  SRR4302545.8931599/94‑1 (MQ=60)

                                                   AAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCG                                         <  SRR4302545.6508379/94‑1 (MQ=60)

                                                   AAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCG                                         <  SRR4302545.10566576/94‑1 (MQ=60)

                                                    AAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGC                                        <  SRR4302545.4176020/94‑1 (MQ=60)

                                                      GACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGC                                      >  SRR4302545.10429050/1‑94 (MQ=60)

                                                      GACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGC                                      >  SRR4302545.270010/1‑94 (MQ=60)

                                                        CAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGT                                    <  SRR4302545.1738348/94‑1 (MQ=60)

                                                        CAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGT                                    >  SRR4302545.10816896/1‑94 (MQ=60)

                                                        CAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGT                                    >  SRR4302545.946711/1‑94 (MQ=60)

                                                         AAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTC                                   >  SRR4302545.8148757/1‑94 (MQ=60)

                                                            CTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTC                                <  SRR4302545.9595551/94‑1 (MQ=60)

                                                            CTCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTC                                <  SRR4302545.9595195/94‑1 (MQ=60)

                                                             TCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCT                               <  SRR4302545.5676652/94‑1 (MQ=60)

                                                             TCGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCT                               >  SRR4302545.9107633/1‑94 (MQ=60)

                                                             TCGAGGCGATGGAAGGCGTGCAGCTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCT                               <  SRR4302545.5986912/94‑1 (MQ=60)

                                                             TAGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCTCTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCT                               <  SRR4302545.5986914/94‑1 (MQ=60)

                                                              CGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTG                              <  SRR4302545.1925283/94‑1 (MQ=60)

                                                              CGAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTG                              <  SRR4302545.5435323/94‑1 (MQ=60)

                                                               GAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGC                             <  SRR4302545.7140228/94‑1 (MQ=60)

                                                               GAGGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGC                             >  SRR4302545.2208497/1‑94 (MQ=60)

                                                                 GGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGC                           >  SRR4302545.7668330/1‑94 (MQ=60)

                                                                 GGCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGC                           >  SRR4302545.7668329/1‑94 (MQ=60)

                                                                  GCGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCT                          >  SRR4302545.9833743/1‑94 (MQ=60)

                                                                   CGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTC                         >  SRR4302545.9320088/1‑94 (MQ=60)

                                                                   CGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTC                         >  SRR4302545.8558552/1‑94 (MQ=60)

                                                                   CGATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTC                         >  SRR4302545.8558553/1‑94 (MQ=60)

                                                                    GATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATTATCGCGCGCCGTCTGCGCTTC                        >  SRR4302545.7143250/1‑94 (MQ=60)

                                                                    GATGGAAGGCGTGCAGTGTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCC                        <  SRR4302545.6949156/94‑1 (MQ=60)

                                                                     ATGGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCG                       >  SRR4302545.5128806/1‑94 (MQ=60)

                                                                       GGAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCA                     <  SRR4302545.2325236/94‑1 (MQ=60)

                                                                        GAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCAT                    >  SRR4302545.7373048/1‑94 (MQ=60)

                                                                        GAAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCAT                    >  SRR4302545.9294406/1‑94 (MQ=60)

                                                                         AAGGCGTGCGGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTCCGATCTGGCCATCATCGCGCGTCGTCCGCGCTCCGCATC                   >  SRR4302545.1117945/1‑94 (MQ=60)

                                                                         AAGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATC                   <  SRR4302545.5758754/94‑1 (MQ=60)

                                                                          AGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCT                  <  SRR4302545.10816099/94‑1 (MQ=60)

                                                                          AGGCGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCT                  <  SRR4302545.8240929/94‑1 (MQ=60)

                                                                             CGTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGAC               >  SRR4302545.9052446/1‑94 (MQ=60)

                                                                              GTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGACC              >  SRR4302545.10312635/1‑94 (MQ=60)

                                                                              GTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGACC              <  SRR4302545.5735288/94‑1 (MQ=60)

                                                                              GTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGACC              <  SRR4302545.6280840/94‑1 (MQ=60)

                                                                              GTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGACC              <  SRR4302545.6323951/94‑1 (MQ=60)

                                                                              GTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGACC              <  SRR4302545.7243069/94‑1 (MQ=60)

                                                                              GTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGACC              <  SRR4302545.4743647/94‑1 (MQ=60)

                                                                              GTGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGACC              >  SRR4302545.8629100/1‑94 (MQ=60)

                                                                               TGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGACCG             <  SRR4302545.7405186/94‑1 (MQ=60)

                                                                               TGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGACCG             <  SRR4302545.820627/94‑1 (MQ=60)

                                                                               TGCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCAGCATCGCGCGTCGTCTGCGCTCCGCATCTGACCG             >  SRR4302545.4553173/1‑94 (MQ=60)

                                                                                GCAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATAGCGCGTCGTCTGCGCTCCGCATCTCACCGT            >  SRR4302545.4618474/1‑94 (MQ=60)

                                                                                 CAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGACCGTA           >  SRR4302545.1419018/1‑94 (MQ=60)

                                                                                 CAGTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGACCGTA           >  SRR4302545.4888675/1‑94 (MQ=60)

                                                                                   GTTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGACCGTAGC         <  SRR4302545.4779136/94‑1 (MQ=60)

                                                                                    TTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGACCGTAGCA        <  SRR4302545.134392/94‑1 (MQ=60)

                                                                                    TTTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGACCGTAGCA        >  SRR4302545.5910990/1‑94 (MQ=60)

                                                                                     TTAAACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGACCGTAGCAA       >  SRR4302545.4577617/1‑94 (MQ=60)

                                                                                        AACGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGACCGTAGCAAATT    >  SRR4302545.2097841/1‑94 (MQ=60)

                                                                                          CGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGACCGTAGCAAATTCT  >  SRR4302545.8318304/1‑94 (MQ=60)

                                                                                          CGGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGACCGTAGCAAATTCT  >  SRR4302545.8318305/1‑94 (MQ=60)

ACCGAAACCCTGAACGAACGCCAGGCGCGCGTGCTGCCGCTTAACGAGCTGAAAGACAAGCTCGAGGCGATGGAAGGCGTGCAGTTTAAACAGTTCAACTCCATTACTGATTACCACTCGCTGATGTTCGATCTGGGCATCATCGCGCGTCGTCTGCGCTCCGCATCTGACCGTAGCAAATTCT  >  NC_000913/975957‑976140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`