Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,424,788 C→T S7S (TCG→TCA)  hisJ ← histidine‑binding periplasmic protein of high‑affinity histidine transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,424,7880CT99.4% 1087.9 / ‑3.6 310S7S (TCG→TCA) hisJhistidine‑binding periplasmic protein of high‑affinity histidine transport system
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (140/165);  total (144/166)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.24e-01

Only 100 of 319 total aligned reads displayed.

GCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAAT  >  NC_000913/2424697‑2424878
                                                                                           |                                                                                          
gcgcATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGa                                                                                          >  1:10004237/1‑94 (MQ=255)
gcgcATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGa                                                                                          <  2:1282748/94‑1 (MQ=255)
 cgcATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGAt                                                                                         >  2:277915/1‑94 (MQ=255)
   cATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAg                                                                                       <  1:9839568/94‑1 (MQ=255)
      aGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCAc                                                                                    <  1:6853850/94‑1 (MQ=255)
       ggtcgggtcgGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCAcc                                                                                   <  1:6530956/94‑1 (MQ=255)
         tcgggtcggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAg                                                                                 >  1:875291/1‑94 (MQ=255)
         tcgggtcggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAg                                                                                 >  2:3330504/1‑94 (MQ=255)
          cgggtcggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGt                                                                                >  2:3125440/1‑94 (MQ=255)
          cgggtcggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGt                                                                                >  1:5894581/1‑94 (MQ=255)
            ggtcggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGttt                                                                              >  1:10341649/1‑94 (MQ=255)
              tcggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGttttt                                                                            <  2:2277614/94‑1 (MQ=255)
                 gTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCa                                                                         >  1:6354609/1‑94 (MQ=255)
                  tACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCAt                                                                        >  2:7049077/1‑94 (MQ=255)
                  tACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCAt                                                                        >  1:9780726/1‑94 (MQ=255)
                    ccGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATaa                                                                      <  1:5424217/94‑1 (MQ=255)
                     cGATGCGGATGTTTTGCGGAATCGCAGCAAACGCTGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAAt                                                                     <  1:1866996/94‑1 (MQ=255)
                     cGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAAt                                                                     >  2:4933677/1‑94 (MQ=255)
                      gATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATg                                                                    <  2:9855508/94‑1 (MQ=255)
                       aTGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGt                                                                   <  1:2528333/94‑1 (MQ=255)
                        tGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTc                                                                  >  2:8111388/1‑94 (MQ=255)
                         gCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTcc                                                                 <  2:10084790/94‑1 (MQ=255)
                          cGGATGTTTTGCAGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCt                                                                <  2:756409/94‑1 (MQ=255)
                           ggATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTc                                                               <  1:2998147/94‑1 (MQ=255)
                            gATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCa                                                              <  1:5318423/94‑1 (MQ=255)
                             aTGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCaa                                                             >  1:4947966/1‑94 (MQ=255)
                             aTGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCaa                                                             >  2:3256178/1‑94 (MQ=255)
                              tGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAAc                                                            >  2:10308364/1‑94 (MQ=255)
                              tGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAAc                                                            <  2:923617/94‑1 (MQ=255)
                               gTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACt                                                           >  2:8357151/1‑94 (MQ=255)
                               gTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACt                                                           <  1:1280035/94‑1 (MQ=255)
                               gTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACt                                                           >  2:6219661/1‑94 (MQ=255)
                               gTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACt                                                           >  2:6746121/1‑94 (MQ=255)
                                ttttGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTg                                                          <  2:4220681/94‑1 (MQ=255)
                                    gCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAg                                                      <  1:8284472/94‑1 (MQ=255)
                                      ggAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTc                                                    >  1:8029580/1‑94 (MQ=255)
                                       gAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGAGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCt                                                   >  1:7344166/1‑94 (MQ=255)
                                        aaTCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCtt                                                  <  2:211673/94‑1 (MQ=255)
                                        aaTCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCtt                                                  <  1:10258272/94‑1 (MQ=255)
                                        aaTCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCtt                                                  >  2:8794345/1‑94 (MQ=255)
                                         aTCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCttt                                                 >  2:4112702/1‑94 (MQ=255)
                                          tCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCtttt                                                >  2:9027693/1‑94 (MQ=255)
                                           cGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCttttt                                               <  1:4308976/94‑1 (MQ=255)
                                            gcagcaAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCtttttt                                              >  2:5509984/1‑93 (MQ=255)
                                              agcaAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTtata                                            >  1:3326288/1‑94 (MQ=255)
                                               gcaAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAg                                           <  1:1099559/94‑1 (MQ=255)
                                                caAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAgg                                          <  2:9043521/94‑1 (MQ=255)
                                                caAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAgg                                          <  2:7404308/94‑1 (MQ=255)
                                                  aaCGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGa                                        >  2:6912406/1‑94 (MQ=255)
                                                  aaCGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGa                                        >  1:5616743/1‑94 (MQ=255)
                                                   aCGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATGGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGaa                                       >  1:2680230/1‑94 (MQ=255)
                                                    cgccgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAat                                      >  1:4012530/1‑94 (MQ=255)
                                                    cgccgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTCCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAat                                      <  2:7215106/94‑1 (MQ=255)
                                                     gccgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAata                                     >  2:5184481/1‑94 (MQ=255)
                                                     gccgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAata                                     >  1:7236443/1‑94 (MQ=255)
                                                      ccgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAatat                                    >  1:5196089/1‑94 (MQ=255)
                                                      ccgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAatat                                    <  2:1046496/94‑1 (MQ=255)
                                                        gcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATAttt                                  <  2:6405232/94‑1 (MQ=255)
                                                        gcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATAttt                                  <  2:6131374/94‑1 (MQ=255)
                                                         cAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTa                                 <  1:3280242/94‑1 (MQ=255)
                                                          aGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTAc                                <  1:4338142/94‑1 (MQ=255)
                                                           gTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACa                               >  1:5728447/1‑94 (MQ=255)
                                                           gTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACa                               <  1:3834144/94‑1 (MQ=255)
                                                           gTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACa                               <  1:7772505/94‑1 (MQ=255)
                                                             tGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTTCAgg                             <  1:353001/94‑1 (MQ=255)
                                                               cgcTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGtt                           <  1:1546065/94‑1 (MQ=255)
                                                               cgcTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGtt                           <  1:6748541/94‑1 (MQ=255)
                                                                gcTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGttt                          >  2:1942837/1‑94 (MQ=255)
                                                                 cTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTg                         >  1:6822366/1‑94 (MQ=255)
                                                                 cTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTg                         <  1:6040386/94‑1 (MQ=255)
                                                                  tGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGt                        <  1:3694555/94‑1 (MQ=255)
                                                                    gagaAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTa                      <  2:4990540/94‑1 (MQ=255)
                                                                    gagaAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTa                      <  2:5445063/94‑1 (MQ=255)
                                                                    gagaAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTa                      >  1:6188965/1‑94 (MQ=255)
                                                                     agaAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTac                     >  1:9538782/1‑94 (MQ=255)
                                                                     agaAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTac                     >  2:4465218/1‑94 (MQ=255)
                                                                     agaAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTac                     <  2:2513998/94‑1 (MQ=255)
                                                                     agaAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTac                     >  1:9256/1‑94 (MQ=255)
                                                                        aGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACt                  <  1:9468081/94‑1 (MQ=255)
                                                                         ggCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACtt                 <  1:8555233/94‑1 (MQ=255)
                                                                         ggCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACtt                 <  2:3631796/94‑1 (MQ=255)
                                                                          gCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTa                >  2:3367137/1‑94 (MQ=255)
                                                                          gCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTa                <  2:1528617/94‑1 (MQ=255)
                                                                             aGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCg             <  1:770761/94‑1 (MQ=255)
                                                                             aGAACCAGAGAGAGGGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCg             >  2:9323866/1‑94 (MQ=255)
                                                                               aaCCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTg           >  1:4119382/1‑94 (MQ=255)
                                                                               aaCCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTg           >  2:1062253/1‑94 (MQ=255)
                                                                                aCCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGc          >  2:8043834/1‑94 (MQ=255)
                                                                                 ccAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGcc         >  2:9434993/1‑94 (MQ=255)
                                                                                    gagagagTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATa      <  2:5625855/94‑1 (MQ=255)
                                                                                    gagagagTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATa      <  1:5480619/94‑1 (MQ=255)
                                                                                    gagagagTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATa      <  1:8961275/94‑1 (MQ=255)
                                                                                     agagagTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATaa     <  1:10519599/94‑1 (MQ=255)
                                                                                     agagagTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATaa     >  2:8763879/1‑94 (MQ=255)
                                                                                     agagagTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATaa     <  1:2272959/94‑1 (MQ=255)
                                                                                     agagagTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATaa     >  2:3896194/1‑94 (MQ=255)
                                                                                     agagagTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATaa     <  1:4634112/94‑1 (MQ=255)
                                                                                       agagTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATaaaa   >  1:3604564/1‑94 (MQ=255)
                                                                                       agagTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATaaaa   >  1:5844394/1‑94 (MQ=255)
                                                                                        gagTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAAt  <  2:10404934/94‑1 (MQ=255)
                                                                                           |                                                                                          
GCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAAT  >  NC_000913/2424697‑2424878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGA  >  NC_000913/2424695‑2424881
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TGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGT                                                                                               >  SRR4302545.4752308/1‑94 (MQ=60)
TGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGT                                                                                               <  SRR4302545.1659586/94‑1 (MQ=60)
 GGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG                                                                                              <  SRR4302545.9845644/94‑1 (MQ=60)
 GGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG                                                                                              <  SRR4302545.7820616/94‑1 (MQ=60)
  GCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGA                                                                                             >  SRR4302545.10300833/1‑94 (MQ=60)
   CGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGAT                                                                                            <  SRR4302545.9176527/94‑1 (MQ=60)
   CGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGAT                                                                                            >  SRR4302545.6578277/1‑94 (MQ=60)
    GCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGACGGCCAGAACCAGAGAGAGTGATA                                                                                           <  SRR4302545.6908945/94‑1 (MQ=60)
    GCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATA                                                                                           <  SRR4302545.6207492/94‑1 (MQ=60)
    GCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATA                                                                                           >  SRR4302545.1265191/1‑94 (MQ=60)
     CATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAG                                                                                          <  SRR4302545.10131926/94‑1 (MQ=60)
     CATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAG                                                                                          <  SRR4302545.9511017/94‑1 (MQ=60)
     CATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAG                                                                                          <  SRR4302545.6962181/94‑1 (MQ=60)
      ATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGC                                                                                         >  SRR4302545.3565972/1‑94 (MQ=60)
      ATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGC                                                                                         <  SRR4302545.4768603/94‑1 (MQ=60)
      ATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGC                                                                                         >  SRR4302545.5722611/1‑94 (MQ=60)
       TAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCA                                                                                        >  SRR4302545.9607943/1‑94 (MQ=60)
        AGGTCTGGTCGGTACCGATGCGGATGTTATGCGGAATCGCATCAAACGCTGCAGTTTCGCTGAAGAATGCCAGAACCAGAGAGAGTGATAGCAC                                                                                       <  SRR4302545.7042126/94‑1 (MQ=60)
        AGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCAC                                                                                       <  SRR4302545.7042131/94‑1 (MQ=60)
         GGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACC                                                                                      >  SRR4302545.2883456/1‑94 (MQ=60)
         GGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACC                                                                                      <  SRR4302545.6709611/94‑1 (MQ=60)
          GTCGGGTCGGTCCCGATGCGGATGTTTTGCTTAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGACCCAGAGAGAGTGATATCACCA                                                                                     <  SRR4302545.6215163/94‑1 (MQ=60)
          GTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCA                                                                                     >  SRR4302545.6916795/1‑94 (MQ=60)
          GTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCA                                                                                     >  SRR4302545.4002096/1‑94 (MQ=60)
          GTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCA                                                                                     >  SRR4302545.8972435/1‑94 (MQ=60)
           TCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAG                                                                                    <  SRR4302545.1378599/94‑1 (MQ=60)
           TCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAG                                                                                    >  SRR4302545.894938/1‑94 (MQ=60)
           TCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAG                                                                                    <  SRR4302545.9631425/94‑1 (MQ=60)
            CGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGT                                                                                   >  SRR4302545.6053463/1‑94 (MQ=60)
            CGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGT                                                                                   >  SRR4302545.6052402/1‑94 (MQ=60)
             GGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTT                                                                                  <  SRR4302545.7525423/94‑1 (MQ=60)
              GGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTT                                                                                 >  SRR4302545.10646492/1‑94 (MQ=60)
              GGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTT                                                                                 >  SRR4302545.10646491/1‑94 (MQ=60)
              GGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTT                                                                                 >  SRR4302545.2671848/1‑94 (MQ=60)
               GTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTT                                                                                <  SRR4302545.8434188/94‑1 (MQ=60)
                 CGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTT                                                                              <  SRR4302545.7716576/94‑1 (MQ=60)
                  GGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCTCCAGTTTTTTC                                                                             >  SRR4302545.5609106/1‑94 (MQ=60)
                  GGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTC                                                                             >  SRR4302545.5609105/1‑94 (MQ=60)
                  GGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTC                                                                             >  SRR4302545.7997965/1‑94 (MQ=60)
                   GTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCA                                                                            >  SRR4302545.6528210/1‑94 (MQ=60)
                    TACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCAT                                                                           >  SRR4302545.9994422/1‑94 (MQ=60)
                    TACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCAT                                                                           <  SRR4302545.4605416/94‑1 (MQ=60)
                    TACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCAT                                                                           >  SRR4302545.8569203/1‑94 (MQ=60)
                    TACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCAT                                                                           >  SRR4302545.10071531/1‑94 (MQ=60)
                    TACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCAT                                                                           >  SRR4302545.10071532/1‑94 (MQ=60)
                     ACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATA                                                                          >  SRR4302545.9212221/1‑94 (MQ=60)
                      CCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAA                                                                         <  SRR4302545.5566014/94‑1 (MQ=60)
                      CCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAA                                                                         <  SRR4302545.4008934/94‑1 (MQ=60)
                       CGATGCGGATGTTTTGCGGAATCGCAGCAAACGCTGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAAT                                                                        <  SRR4302545.1912727/94‑1 (MQ=60)
                       CGATGCGAATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAAT                                                                        >  SRR4302545.10732848/1‑94 (MQ=60)
                         ATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGT                                                                      <  SRR4302545.526781/94‑1 (MQ=60)
                         ATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGT                                                                      <  SRR4302545.2589429/94‑1 (MQ=60)
                         AGGCGGATGTTATGCGAAATCGCAGCAAACACCGTAGTTGCCCTGAAGAATACCAGAACAAGAGAGAGTGATAGCACCAGTTTTTTCATAATGT                                                                      <  SRR4302545.7509067/94‑1 (MQ=38)
                           GCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCC                                                                    >  SRR4302545.7415529/1‑94 (MQ=60)
                            CGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCT                                                                   <  SRR4302545.925966/94‑1 (MQ=60)
                             GGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTC                                                                  <  SRR4302545.3071266/94‑1 (MQ=60)
                             GGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTC                                                                  >  SRR4302545.1748212/1‑94 (MQ=60)
                              GATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCA                                                                 <  SRR4302545.5456363/94‑1 (MQ=60)
                              GATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCA                                                                 <  SRR4302545.5456364/94‑1 (MQ=60)
                               ATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAA                                                                >  SRR4302545.606589/1‑94 (MQ=60)
                               ATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAA                                                                >  SRR4302545.5073364/1‑94 (MQ=60)
                                TGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAAC                                                               <  SRR4302545.2454585/94‑1 (MQ=60)
                                TGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAAC                                                               >  SRR4302545.6248173/1‑94 (MQ=60)
                                 GTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACT                                                              <  SRR4302545.1308571/94‑1 (MQ=60)
                                    TTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGAC                                                           <  SRR4302545.3790710/94‑1 (MQ=60)
                                     TGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACA                                                          >  SRR4302545.8066072/1‑94 (MQ=60)
                                      GCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAG                                                         <  SRR4302545.8524450/94‑1 (MQ=60)
                                       CGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGT                                                        >  SRR4302545.5966379/1‑94 (MQ=60)
                                       CGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGA                                                        <  SRR4302545.10418215/94‑1 (MQ=60)
                                       CGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGAGATAGCACCCGGTTTTTCATAATGTCCTCAACTGACAGT                                                        >  SRR4302545.7942154/1‑94 (MQ=60)
                                        GGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTC                                                       >  SRR4302545.7761831/1‑94 (MQ=60)
                                        GGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTC                                                       >  SRR4302545.2103424/1‑94 (MQ=60)
                                        GGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTC                                                       >  SRR4302545.8258317/1‑94 (MQ=60)
                                         GAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCT                                                      <  SRR4302545.442944/94‑1 (MQ=60)
                                         GAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGAGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCT                                                      >  SRR4302545.7550383/1‑94 (MQ=60)
                                          AATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTT                                                     <  SRR4302545.10561014/94‑1 (MQ=60)
                                          AATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTT                                                     <  SRR4302545.10561015/94‑1 (MQ=60)
                                           ATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTT                                                    <  SRR4302545.5488755/94‑1 (MQ=60)
                                            TCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTT                                                   >  SRR4302545.4405423/1‑94 (MQ=60)
                                            TCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTT                                                   <  SRR4302545.5782971/94‑1 (MQ=60)
                                             CGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTT                                                  <  SRR4302545.4417489/94‑1 (MQ=60)
                                             CGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTT                                                  <  SRR4302545.4417488/94‑1 (MQ=60)
                                             CGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTT                                                  <  SRR4302545.9818615/94‑1 (MQ=60)
                                             CGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTT                                                  <  SRR4302545.8661800/94‑1 (MQ=60)
                                             CGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTT                                                  <  SRR4302545.2279944/94‑1 (MQ=60)
                                              GCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTA                                                 <  SRR4302545.8439547/94‑1 (MQ=60)
                                                AGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATA                                               >  SRR4302545.3407598/1‑94 (MQ=60)
                                                 GCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAG                                              <  SRR4302545.2722563/94‑1 (MQ=60)
                                                 GCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAG                                              <  SRR4302545.1123461/94‑1 (MQ=60)
                                                  CAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGG                                             <  SRR4302545.5333995/94‑1 (MQ=60)
                                                  CAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGG                                             >  SRR4302545.4678372/1‑94 (MQ=60)
                                                   AAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGG                                            <  SRR4302545.5046596/94‑1 (MQ=60)
                                                    AACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGA                                           >  SRR4302545.5765926/1‑94 (MQ=60)
                                                     ACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATGGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAA                                          >  SRR4302545.2744812/1‑94 (MQ=60)
                                                     ACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAA                                          >  SRR4302545.5214205/1‑94 (MQ=60)
                                                     ACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAA                                          >  SRR4302545.4152365/1‑94 (MQ=60)
                                                      CGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAAT                                         <  SRR4302545.10064898/94‑1 (MQ=60)
                                                      CGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAAT                                         >  SRR4302545.4112904/1‑94 (MQ=60)
                                                       GCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATA                                        >  SRR4302545.7011462/1‑94 (MQ=60)
                                                       GCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATA                                        >  SRR4302545.7438773/1‑94 (MQ=60)
                                                        CCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATAT                                       >  SRR4302545.2980369/1‑94 (MQ=60)
                                                        CCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATAT                                       <  SRR4302545.3647998/94‑1 (MQ=60)
                                                        CCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATAT                                       <  SRR4302545.3647999/94‑1 (MQ=60)
                                                        CCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATAT                                       >  SRR4302545.5329917/1‑94 (MQ=60)
                                                         CGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATT                                      >  SRR4302545.336446/1‑94 (MQ=60)
                                                         CGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATT                                      >  SRR4302545.336447/1‑94 (MQ=60)
                                                          GCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT                                     <  SRR4302545.74019/94‑1 (MQ=60)
                                                           CAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTA                                    >  SRR4302545.1685232/1‑94 (MQ=60)
                                                           CAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTA                                    <  SRR4302545.3360636/94‑1 (MQ=60)
                                                            AGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTAC                                   >  SRR4302545.4169030/1‑94 (MQ=60)
                                                            AGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTAC                                   <  SRR4302545.1135815/94‑1 (MQ=60)
                                                            AGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTAC                                   <  SRR4302545.4447601/94‑1 (MQ=60)
                                                             GTTGCGCTGGAGAAGGCCAGAACCCGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATTGGGAATATTTACC                                  >  SRR4302545.5882151/1‑94 (MQ=60)
                                                             GTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACA                                  <  SRR4302545.7992505/94‑1 (MQ=60)
                                                             GTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACA                                  <  SRR4302545.3118337/94‑1 (MQ=60)
                                                             GTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACA                                  <  SRR4302545.3929736/94‑1 (MQ=60)
                                                             GTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACA                                  >  SRR4302545.5882152/1‑94 (MQ=60)
                                                              TTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAG                                 <  SRR4302545.6161345/94‑1 (MQ=60)
                                                              TTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAG                                 <  SRR4302545.6161347/94‑1 (MQ=60)
                                                               TGCGCTGGAGAAGGCGAGAACCAGAGAGAGTGATAGCGCCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGG                                <  SRR4302545.1093649/94‑1 (MQ=60)
                                                               TGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTTCAGG                                <  SRR4302545.360601/94‑1 (MQ=60)
                                                               TGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGG                                >  SRR4302545.3733984/1‑94 (MQ=60)
                                                               TGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGG                                >  SRR4302545.2910660/1‑94 (MQ=60)
                                                               TGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGG                                >  SRR4302545.10265061/1‑94 (MQ=60)
                                                                GCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGT                               <  SRR4302545.3823056/94‑1 (MQ=60)
                                                                GCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGT                               <  SRR4302545.2335165/94‑1 (MQ=60)
                                                                 CGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTT                              <  SRR4302545.1582368/94‑1 (MQ=60)
                                                                 CGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTT                              <  SRR4302545.6410185/94‑1 (MQ=60)
                                                                 CGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTT                              <  SRR4302545.6933603/94‑1 (MQ=60)
                                                                   CTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTG                            >  SRR4302545.7009837/1‑94 (MQ=60)
                                                                   CTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTG                            <  SRR4302545.6203777/94‑1 (MQ=60)
                                                                    TGGAGGAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGGCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGT                           <  SRR4302545.3785023/94‑1 (MQ=60)
                                                                    TGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGT                           <  SRR4302545.7006962/94‑1 (MQ=60)
                                                                    TGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGT                           <  SRR4302545.3785024/94‑1 (MQ=60)
                                                                    TGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGT                           <  SRR4302545.3785025/94‑1 (MQ=60)
                                                                    TGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGT                           >  SRR4302545.156125/1‑94 (MQ=60)
                                                                     GGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTT                          <  SRR4302545.10885844/94‑1 (MQ=60)
                                                                      GAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTA                         >  SRR4302545.6357387/1‑94 (MQ=60)
                                                                      GAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTA                         <  SRR4302545.10637751/94‑1 (MQ=60)
                                                                       AGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTAC                        >  SRR4302545.9819701/1‑94 (MQ=60)
                                                                       AGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTAC                        >  SRR4302545.9476/1‑94 (MQ=60)
                                                                        GAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACA                       >  SRR4302545.6380135/1‑94 (MQ=60)
                                                                         AAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACAC                      <  SRR4302545.7273058/94‑1 (MQ=60)
                                                                          AGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACT                     <  SRR4302545.9746523/94‑1 (MQ=60)
                                                                           GGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTT                    <  SRR4302545.3187368/94‑1 (MQ=60)
                                                                           GGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTT                    <  SRR4302545.8802577/94‑1 (MQ=60)
                                                                           GGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTT                    >  SRR4302545.6641180/1‑94 (MQ=60)
                                                                           GGCCAGAACCAGAGAGAGTGATACCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTT                    <  SRR4302545.6017739/94‑1 (MQ=60)
                                                                            GCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTA                   <  SRR4302545.4237089/94‑1 (MQ=60)
                                                                              CAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATGTTTACAGGTTTGTTACACTTATC                 >  SRR4302545.5963082/1‑94 (MQ=60)
                                                                              CAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATC                 >  SRR4302545.10632447/1‑94 (MQ=60)
                                                                               AGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCG                <  SRR4302545.5723458/94‑1 (MQ=60)
                                                                               AGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCG                <  SRR4302545.788079/94‑1 (MQ=60)
                                                                                GAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGT               >  SRR4302545.8072457/1‑94 (MQ=60)
                                                                                GAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGT               >  SRR4302545.10108437/1‑94 (MQ=60)
                                                                                 AACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATCGGGAATATTTACAGGTTTGTTACACTTATCGTG              >  SRR4302545.9806426/1‑94 (MQ=60)
                                                                                 AACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTG              >  SRR4302545.4222447/1‑94 (MQ=60)
                                                                                 AACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTG              >  SRR4302545.5607148/1‑94 (MQ=60)
                                                                                 AACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTG              >  SRR4302545.7935393/1‑94 (MQ=60)
                                                                                 AACCAGAGAGAGTGATAGCAACAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAAGATTTACAGGTTTGTTACACTTATCGTG              <  SRR4302545.8935286/94‑1 (MQ=60)
                                                                                    CAGAGAGCGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCA           >  SRR4302545.5385453/1‑94 (MQ=60)
                                                                                    CAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCA           >  SRR4302545.6995127/1‑94 (MQ=60)
                                                                                    CAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCA           <  SRR4302545.9352922/94‑1 (MQ=60)
                                                                                     AGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCAT          >  SRR4302545.354097/1‑94 (MQ=60)
                                                                                      GAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATA         <  SRR4302545.1571551/94‑1 (MQ=60)
                                                                                      GAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATA         <  SRR4302545.9221864/94‑1 (MQ=60)
                                                                                      GAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATA         <  SRR4302545.5624608/94‑1 (MQ=60)
                                                                                       AGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAA        <  SRR4302545.2328228/94‑1 (MQ=60)
                                                                                       AGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAA        <  SRR4302545.10829549/94‑1 (MQ=60)
                                                                                       AGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAA        >  SRR4302545.4708426/1‑94 (MQ=60)
                                                                                       AGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAA        <  SRR4302545.4752542/94‑1 (MQ=60)
                                                                                         AGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAA      >  SRR4302545.3692455/1‑94 (MQ=60)
                                                                                         AGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAA      >  SRR4302545.6001770/1‑94 (MQ=60)
                                                                                           AGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATT    >  SRR4302545.9913340/1‑94 (MQ=60)
                                                                                            GTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTG   <  SRR4302545.8811319/94‑1 (MQ=60)
                                                                                            GTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTG   <  SRR4302545.8811318/94‑1 (MQ=60)
                                                                                            GTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTG   >  SRR4302545.1571343/1‑94 (MQ=60)
                                                                                            GTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTG   >  SRR4302545.1571342/1‑94 (MQ=60)
                                                                                             TGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGA  >  SRR4302545.10088517/1‑94 (MQ=60)
                                                                                             TGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGA  >  SRR4302545.972752/1‑94 (MQ=60)
                                                                                             |                                                                                             
TGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGA  >  NC_000913/2424695‑2424881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑