Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,424,788 C→T S7S (TCG→TCA)  hisJ ← histidine‑binding periplasmic protein of high‑affinity histidine transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,424,7880CT99.1% 795.7 / NA 232S7S (TCG→TCA) hisJhistidine‑binding periplasmic protein of high‑affinity histidine transport system
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (97/132);  total (99/133)
Fisher's exact test for biased strand distribution p-value = 1.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 236 total aligned reads displayed.

CGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAA  >  NC_000913/2424698‑2424877
                                                                                          |                                                                                          
cgcATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑a                                                                                         <  1:3609501/93‑1 (MQ=255)
cgcATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑a                                                                                         <  1:832608/93‑1 (MQ=255)
 gcATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑At                                                                                        >  1:3730508/1‑93 (MQ=255)
 gcATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑At                                                                                        >  1:4087030/1‑93 (MQ=255)
 gcATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑At                                                                                        <  2:5046922/93‑1 (MQ=255)
   aTAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAg                                                                                      <  1:884374/93‑1 (MQ=255)
     aGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCa                                                                                    >  2:4334016/1‑93 (MQ=255)
      ggtcgggtcgGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCAc                                                                                   >  2:2587531/1‑93 (MQ=255)
        tcgggtcggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCa                                                                                 >  2:2784534/1‑93 (MQ=255)
         cgggtcggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAg                                                                                <  2:483282/93‑1 (MQ=255)
         cgggtcggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAg                                                                                <  1:2230241/93‑1 (MQ=255)
          gggtcggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGt                                                                               >  1:660114/1‑93 (MQ=255)
          gggtcggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGt                                                                               <  1:255449/93‑1 (MQ=255)
              cggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGttttt                                                                           >  2:2883722/1‑93 (MQ=255)
                gTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTc                                                                         >  2:3317170/1‑93 (MQ=255)
                gTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTc                                                                         >  1:4774056/1‑93 (MQ=255)
                 tACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCa                                                                        <  2:2198383/93‑1 (MQ=255)
                 tACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCa                                                                        >  1:80588/1‑93 (MQ=255)
                 tACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCa                                                                        <  2:1939128/93‑1 (MQ=255)
                 tACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCa                                                                        <  2:5906420/93‑1 (MQ=255)
                  aCCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCAt                                                                       <  2:1735938/93‑1 (MQ=255)
                    cGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATaa                                                                     <  1:2534276/93‑1 (MQ=255)
                     gATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAAt                                                                    >  2:2590333/1‑93 (MQ=255)
                     gATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAAt                                                                    <  2:1478891/93‑1 (MQ=255)
                      aTGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATg                                                                   >  2:674379/1‑93 (MQ=255)
                      aTGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATg                                                                   >  1:5824522/1‑93 (MQ=255)
                       tGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGt                                                                  >  2:3922737/1‑93 (MQ=255)
                        gCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTc                                                                 >  1:792983/1‑93 (MQ=255)
                          ggATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGCACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCt                                                               >  1:5168691/1‑93 (MQ=255)
                           gATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGATAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTc                                                              >  1:3215153/1‑93 (MQ=255)
                           gATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTc                                                              <  1:4495782/93‑1 (MQ=255)
                           gATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTc                                                              >  1:4598427/1‑93 (MQ=255)
                            aTGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCa                                                             <  2:1153304/93‑1 (MQ=255)
                            aTGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCa                                                             <  2:6232981/93‑1 (MQ=255)
                             tGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCaa                                                            >  1:2793304/1‑93 (MQ=255)
                             tGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCaa                                                            >  2:1859903/1‑93 (MQ=255)
                              gTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAAc                                                           >  2:3050891/1‑93 (MQ=255)
                               ttttGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACt                                                          <  1:3417825/93‑1 (MQ=255)
                                   gCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACa                                                      <  1:1191230/93‑1 (MQ=255)
                                    cGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAg                                                     <  1:2645235/93‑1 (MQ=255)
                                     ggAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGt                                                    <  1:208788/93‑1 (MQ=255)
                                        aTCGCATGAAACGCCGGAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCtt                                                 <  2:4801945/93‑1 (MQ=255)
                                        aTCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCtt                                                 <  1:1669479/93‑1 (MQ=255)
                                        aTCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCtt                                                 <  1:6130044/93‑1 (MQ=255)
                                         tCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGATAGTCttt                                                <  2:6140323/93‑1 (MQ=255)
                                         tCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCttt                                                <  1:6401418/93‑1 (MQ=255)
                                          cGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCtttt                                               <  2:704173/93‑1 (MQ=255)
                                          cGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCtttt                                               <  1:5954864/93‑1 (MQ=255)
                                          cGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCtttt                                               <  1:5900290/93‑1 (MQ=255)
                                           gcagcaAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCttttt                                              <  1:6003703/93‑1 (MQ=255)
                                              gcaAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTtata                                           <  2:165647/93‑1 (MQ=255)
                                               caAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAg                                          >  1:4032050/1‑93 (MQ=255)
                                               caAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAg                                          <  2:5171231/93‑1 (MQ=255)
                                               caAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAg                                          >  1:1579943/1‑93 (MQ=255)
                                                aaaCGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAgg                                         <  2:4532411/93‑1 (MQ=255)
                                                   cgccgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGaa                                      <  1:24536/93‑1 (MQ=255)
                                                    gccgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAat                                     <  2:5483701/93‑1 (MQ=255)
                                                    gccgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAat                                     <  1:496551/93‑1 (MQ=255)
                                                     ccgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAata                                    >  2:3788993/1‑93 (MQ=255)
                                                     ccgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAata                                    >  1:4343621/1‑93 (MQ=255)
                                                     cagcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAata                                    <  2:4275811/91‑1 (MQ=255)
                                                       gcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATAtt                                  <  2:1542900/93‑1 (MQ=255)
                                                       gcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATAtt                                  <  1:4009991/93‑1 (MQ=255)
                                                       gcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATAtt                                  >  1:416829/1‑93 (MQ=255)
                                                        cAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATAttt                                 <  1:1084327/93‑1 (MQ=255)
                                                         aGTTGCGCTGGAAGAGGCCAGAACCAGAG‑GAGGGTATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTa                                <  2:6171452/93‑1 (MQ=255)
                                                          gTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTAc                               >  2:3360561/1‑93 (MQ=255)
                                                           ttGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACa                              <  2:1057424/93‑1 (MQ=255)
                                                           ttGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACa                              >  1:1837650/1‑93 (MQ=255)
                                                           ttGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACa                              >  1:2250972/1‑93 (MQ=255)
                                                           ttGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACa                              >  1:1504910/1‑93 (MQ=255)
                                                            tGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAg                             <  1:3043824/93‑1 (MQ=255)
                                                            tGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAg                             <  1:3270393/93‑1 (MQ=255)
                                                             gcgcTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAgg                            <  2:5187209/93‑1 (MQ=255)
                                                               gcTGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGtt                          <  1:5502693/93‑1 (MQ=255)
                                                                 tGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTg                        >  1:6065901/1‑93 (MQ=255)
                                                                 tGGAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTg                        >  2:4082569/1‑93 (MQ=255)
                                                                  ggAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGt                       <  2:2073168/93‑1 (MQ=255)
                                                                  ggAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGt                       <  1:4695472/93‑1 (MQ=255)
                                                                  ggAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGt                       <  2:4663807/93‑1 (MQ=255)
                                                                  ggAGAAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGt                       >  2:4999973/1‑93 (MQ=255)
                                                                   gagaAGGCCAGAACCAGAGAGAGTG‑ATAGCACAAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGtt                      >  1:1333176/1‑93 (MQ=255)
                                                                    agaAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTa                     <  1:4996193/93‑1 (MQ=255)
                                                                    agaAGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTa                     <  1:4071569/93‑1 (MQ=255)
                                                                      aaGGCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTaca                   >  1:4372131/1‑93 (MQ=255)
                                                                        ggCCAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACt                 >  1:5418717/1‑93 (MQ=255)
                                                                          ccAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTa               >  1:4416697/1‑93 (MQ=255)
                                                                           cAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTAt              <  1:1001791/93‑1 (MQ=255)
                                                                           cAGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTAt              >  2:2480556/1‑93 (MQ=255)
                                                                            aGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATc             <  2:3277691/93‑1 (MQ=255)
                                                                            aGAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATc             >  1:3854246/1‑93 (MQ=255)
                                                                             gAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCg            <  2:5215749/93‑1 (MQ=255)
                                                                             gAACCAGAGAGAGTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCg            <  2:3460778/93‑1 (MQ=255)
                                                                                  agagagagTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCa       <  1:4846231/93‑1 (MQ=255)
                                                                                  agagagagTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCa       >  2:5345459/1‑93 (MQ=255)
                                                                                  agagagagTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCa       >  2:596201/1‑93 (MQ=255)
                                                                                   gagagagTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCAt      <  2:5662752/93‑1 (MQ=255)
                                                                                   gagagagTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCAt      >  2:3091665/1‑93 (MQ=255)
                                                                                     gagagTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTTTTACACTTATCGTGCCATaa    >  1:1689677/1‑93 (MQ=255)
                                                                                       gagTG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATaaaa  >  2:3621669/1‑93 (MQ=255)
                                                                                          |                                                                                          
CGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCG‑ATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAA  >  NC_000913/2424698‑2424877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGA  >  NC_000913/2424695‑2424881
                                                                                             |                                                                                             
TGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCCCTGGAGAAGGCAAGAACCAGAGAGAGG                                                                                               >  SRR4302537.3237960/1‑94 (MQ=60)
 GGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTG                                                                                              <  SRR4302537.5500445/94‑1 (MQ=60)
  GCGCATAGGTCGGGTCGGTACCGATGCGTATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGA                                                                                             <  SRR4302537.355622/94‑1 (MQ=60)
   CGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGAT                                                                                            >  SRR4302537.4115569/1‑94 (MQ=60)
   CGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGAT                                                                                            >  SRR4302537.3756713/1‑94 (MQ=60)
   CGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGAT                                                                                            <  SRR4302537.3634945/94‑1 (MQ=60)
   CGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGAT                                                                                            <  SRR4302537.838304/94‑1 (MQ=60)
    GCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATA                                                                                           <  SRR4302537.4416080/94‑1 (MQ=60)
      ATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGC                                                                                         <  SRR4302537.890460/94‑1 (MQ=60)
         GGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACC                                                                                      <  SRR4302537.5028983/94‑1 (MQ=60)
           GCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAG                                                                                    <  SRR4302537.4798652/94‑1 (MQ=60)
           GCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAG                                                                                    <  SRR4302537.686250/94‑1 (MQ=60)
           GCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAG                                                                                    <  SRR4302537.6044517/94‑1 (MQ=60)
            CGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGT                                                                                   >  SRR4302537.664611/1‑94 (MQ=60)
            CGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGT                                                                                   <  SRR4302537.2245971/94‑1 (MQ=60)
             GGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTT                                                                                  <  SRR4302537.257260/94‑1 (MQ=60)
             GGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTT                                                                                  >  SRR4302537.339177/1‑94 (MQ=60)
             GGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTT                                                                                  >  SRR4302537.1479276/1‑94 (MQ=60)
               GTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTT                                                                                >  SRR4302537.3428278/1‑94 (MQ=60)
                TCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTT                                                                               >  SRR4302537.5058419/1‑94 (MQ=60)
                  GGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTC                                                                             >  SRR4302537.1048232/1‑94 (MQ=60)
                  GGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTC                                                                             >  SRR4302537.4807016/1‑94 (MQ=60)
                  GGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTC                                                                             >  SRR4302537.4811174/1‑94 (MQ=60)
                   GTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCA                                                                            >  SRR4302537.81180/1‑94 (MQ=60)
                     ACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATA                                                                          >  SRR4302537.1214419/1‑94 (MQ=60)
                      CCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAA                                                                         <  SRR4302537.4179585/94‑1 (MQ=60)
                       CGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAAT                                                                        <  SRR4302537.2552061/94‑1 (MQ=60)
                       CGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAAT                                                                        >  SRR4302537.2796501/1‑94 (MQ=60)
                       CGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAAT                                                                        >  SRR4302537.808619/1‑94 (MQ=60)
                       CGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAAT                                                                        >  SRR4302537.813180/1‑94 (MQ=60)
                        GATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATG                                                                       >  SRR4302537.5864438/1‑94 (MQ=60)
                        GATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATG                                                                       >  SRR4302537.4113923/1‑94 (MQ=60)
                        GATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATG                                                                       >  SRR4302537.3253758/1‑94 (MQ=60)
                         ATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGT                                                                      <  SRR4302537.2556463/94‑1 (MQ=60)
                         ATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAACACCAGTTTTTTCATAATGT                                                                      >  SRR4302537.4335089/1‑94 (MQ=60)
                          TGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTC                                                                     >  SRR4302537.798428/1‑94 (MQ=60)
                            CGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGCACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCT                                                                   >  SRR4302537.5204289/1‑94 (MQ=60)
                             GGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGATAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTC                                                                  >  SRR4302537.3237780/1‑94 (MQ=60)
                             GGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTC                                                                  >  SRR4302537.2947237/1‑94 (MQ=60)
                             GGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTC                                                                  >  SRR4302537.4630250/1‑94 (MQ=60)
                              GATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCA                                                                 <  SRR4302537.4526883/94‑1 (MQ=60)
                               ATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAA                                                                >  SRR4302537.2812826/1‑94 (MQ=60)
                               ATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAA                                                                <  SRR4302537.4158029/94‑1 (MQ=60)
                                 GTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACT                                                              <  SRR4302537.4478690/94‑1 (MQ=60)
                                  TTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTG                                                             <  SRR4302537.3442020/94‑1 (MQ=60)
                                    TTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCACAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGAC                                                           <  SRR4302537.4576542/94‑1 (MQ=60)
                                      GCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAG                                                         <  SRR4302537.5061841/94‑1 (MQ=60)
                                      GCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAG                                                         <  SRR4302537.1199501/94‑1 (MQ=60)
                                       CGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGT                                                        <  SRR4302537.2663737/94‑1 (MQ=60)
                                        GGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTC                                                       <  SRR4302537.210288/94‑1 (MQ=60)
                                        GGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTC                                                       >  SRR4302537.5992163/1‑94 (MQ=60)
                                        GGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTC                                                       <  SRR4302537.6059255/94‑1 (MQ=60)
                                           ATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTT                                                    <  SRR4302537.6172181/94‑1 (MQ=60)
                                           ATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTT                                                    <  SRR4302537.1681174/94‑1 (MQ=60)
                                            TCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTT                                                   <  SRR4302537.6444995/94‑1 (MQ=60)
                                             CGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTT                                                  <  SRR4302537.5940778/94‑1 (MQ=60)
                                             CGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTT                                                  <  SRR4302537.5995742/94‑1 (MQ=60)
                                              GCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTA                                                 >  SRR4302537.4374002/1‑94 (MQ=60)
                                              GCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTA                                                 <  SRR4302537.6044946/94‑1 (MQ=60)
                                                 GCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAG                                              <  SRR4302537.2590161/94‑1 (MQ=60)
                                                 GCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAG                                              >  SRR4302537.4060270/1‑94 (MQ=60)
                                                 GCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAG                                              >  SRR4302537.1590983/1‑94 (MQ=60)
                                                 GCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAG                                              <  SRR4302537.1166438/94‑1 (MQ=60)
                                                 ACAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAG                                              <  SRR4302537.5802264/94‑1 (MQ=60)
                                                   AAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGG                                            >  SRR4302537.2464060/1‑94 (MQ=60)
                                                    AACGCCGCAGTTGTGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTCTTTATAGGGA                                           <  SRR4302537.2241243/94‑1 (MQ=60)
                                                    AACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGA                                           <  SRR4302537.4096354/94‑1 (MQ=60)
                                                      CGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAAT                                         <  SRR4302537.5461266/94‑1 (MQ=60)
                                                      CGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAAT                                         <  SRR4302537.24730/94‑1 (MQ=60)
                                                      CGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAAT                                         >  SRR4302537.4901151/1‑94 (MQ=60)
                                                      CGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTATTTATAGGGAAT                                         <  SRR4302537.6027011/94‑1 (MQ=60)
                                                       GCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATA                                        >  SRR4302537.4373743/1‑94 (MQ=60)
                                                       GCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATA                                        <  SRR4302537.499968/94‑1 (MQ=60)
                                                         CGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATT                                      >  SRR4302537.419724/1‑94 (MQ=60)
                                                          GCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT                                     <  SRR4302537.646045/94‑1 (MQ=60)
                                                          GCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTT                                     <  SRR4302537.4038064/94‑1 (MQ=60)
                                                           CAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTA                                    <  SRR4302537.403915/94‑1 (MQ=60)
                                                           CAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTA                                    <  SRR4302537.1091809/94‑1 (MQ=60)
                                                            AGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTAC                                   >  SRR4302537.4885318/1‑94 (MQ=60)
                                                            AGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTAC                                   >  SRR4302537.5209479/1‑94 (MQ=60)
                                                            AGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTAC                                   >  SRR4302537.1249284/1‑94 (MQ=60)
                                                             GTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACA                                  <  SRR4302537.4424706/94‑1 (MQ=60)
                                                             GTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACA                                  <  SRR4302537.3517353/94‑1 (MQ=60)
                                                             GTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACA                                  >  SRR4302537.2266860/1‑94 (MQ=60)
                                                             GTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACA                                  >  SRR4302537.1850442/1‑94 (MQ=60)
                                                             GTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACA                                  >  SRR4302537.1515410/1‑94 (MQ=60)
                                                              TTGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAG                                 <  SRR4302537.3918006/94‑1 (MQ=60)
                                                               TGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGG                                <  SRR4302537.3293474/94‑1 (MQ=60)
                                                               TGCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGG                                <  SRR4302537.3065030/94‑1 (MQ=60)
                                                                GCGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGT                               <  SRR4302537.932195/94‑1 (MQ=60)
                                                                 CGCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTT                              <  SRR4302537.4726421/94‑1 (MQ=60)
                                                                  GCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTT                             >  SRR4302537.314408/1‑94 (MQ=60)
                                                                  GCTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTT                             <  SRR4302537.5540266/94‑1 (MQ=60)
                                                                   CTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTG                            >  SRR4302537.6107571/1‑94 (MQ=60)
                                                                   CTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTG                            <  SRR4302537.18571/94‑1 (MQ=60)
                                                                   CTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTG                            <  SRR4302537.1684538/94‑1 (MQ=60)
                                                                   CTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTG                            <  SRR4302537.1671323/94‑1 (MQ=60)
                                                                   CTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTG                            >  SRR4302537.1650128/1‑94 (MQ=60)
                                                                   CTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTG                            >  SRR4302537.862004/1‑94 (MQ=60)
                                                                     GGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTT                          <  SRR4302537.4727953/94‑1 (MQ=60)
                                                                     GGAGAAGGCCAGAACCAGAGAGAGTGATAGCACAAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTT                          >  SRR4302537.1342461/1‑94 (MQ=60)
                                                                       AGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTAC                        <  SRR4302537.4100001/94‑1 (MQ=60)
                                                                       AGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTAC                        >  SRR4302537.752172/1‑94 (MQ=60)
                                                                       AGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTAC                        <  SRR4302537.5030625/94‑1 (MQ=60)
                                                                       AGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTAC                        <  SRR4302537.247895/94‑1 (MQ=60)
                                                                       AGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTAC                        >  SRR4302537.225330/1‑94 (MQ=60)
                                                                        GAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACA                       >  SRR4302537.4402337/1‑94 (MQ=60)
                                                                        GAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACA                       <  SRR4302537.6397140/94‑1 (MQ=60)
                                                                         AAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACAC                      <  SRR4302537.2566571/94‑1 (MQ=60)
                                                                          AGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACT                     >  SRR4302537.5455651/1‑94 (MQ=60)
                                                                          AGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACT                     <  SRR4302537.5910329/94‑1 (MQ=60)
                                                                            GCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTA                   >  SRR4302537.4447187/1‑94 (MQ=60)
                                                                            GCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTA                   <  SRR4302537.4723114/94‑1 (MQ=60)
                                                                             CCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTAT                  <  SRR4302537.4043424/94‑1 (MQ=60)
                                                                             CCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTAT                  >  SRR4302537.6150754/1‑94 (MQ=60)
                                                                              CAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATC                 <  SRR4302537.1008703/94‑1 (MQ=60)
                                                                              CAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATC                 <  SRR4302537.2408850/94‑1 (MQ=60)
                                                                              CAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATC                 >  SRR4302537.3881229/1‑94 (MQ=60)
                                                                                GAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGT               >  SRR4302537.88391/1‑94 (MQ=60)
                                                                                 AACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTG              >  SRR4302537.3877269/1‑94 (MQ=60)
                                                                                 AACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTG              >  SRR4302537.3710165/1‑94 (MQ=60)
                                                                                  ACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGC             >  SRR4302537.628627/1‑94 (MQ=60)
                                                                                    CAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCA           >  SRR4302537.2080266/1‑94 (MQ=60)
                                                                                    CAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGCTACACTTATCGTGCCA           >  SRR4302537.1840530/1‑94 (MQ=60)
                                                                                     AGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCAT          <  SRR4302537.4879675/94‑1 (MQ=60)
                                                                                     AGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCAT          >  SRR4302537.3859997/1‑94 (MQ=60)
                                                                                       AGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTTTTACACTTATCGTGCCATAA        >  SRR4302537.1701506/1‑94 (MQ=60)
                                                                                       AGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAA        >  SRR4302537.5648787/1‑94 (MQ=60)
                                                                                         AGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAA      <  SRR4302537.1551162/94‑1 (MQ=60)
                                                                                          GAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAAT     <  SRR4302537.3004840/94‑1 (MQ=60)
                                                                                           AGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATT    >  SRR4302537.3118323/1‑94 (MQ=60)
                                                                                           AGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATT    >  SRR4302537.876659/1‑94 (MQ=60)
                                                                                            GTGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTG   <  SRR4302537.701967/94‑1 (MQ=60)
                                                                                             TGATAGCACCAGTTCTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGA  <  SRR4302537.1016540/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGA  >  NC_000913/2424695‑2424881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑