Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,766,427 2 bp→AC coding (697‑698/864 nt) b2632 → putative GTP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,766,4270TA100.0% 1027.5 / NA 280W233T (TGG→ACG) b2632putative GTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (135/145);  total (135/145)
*NC_0009132,766,4280GC100.0% 935.8 / NA 280W233T (TGG→ACG) b2632putative GTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (135/145);  total (135/145)

Only 100 of 281 total aligned reads displayed.

AAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGCCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATTCC  >  NC_000913/2766339‑2766512
                                                                                        ||                                                                                    
aaGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTa                                                                                  <  2:3039445/94‑1 (MQ=25)
aaGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTa                                                                                  >  1:6926051/1‑94 (MQ=25)
 aGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAg                                                                                 >  2:5404912/1‑94 (MQ=21)
     tGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCg                                                                             <  2:332399/94‑1 (MQ=25)
      gATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCgg                                                                            >  2:8649246/1‑94 (MQ=25)
       aTGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCggg                                                                           <  2:2775163/94‑1 (MQ=25)
       aTGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCggg                                                                           <  2:6402695/94‑1 (MQ=25)
        tGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGa                                                                          >  1:7294263/1‑94 (MQ=21)
        tGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGa                                                                          <  1:7426715/94‑1 (MQ=21)
        tGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGa                                                                          >  2:8188601/1‑94 (MQ=25)
         ggCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAg                                                                         <  2:1917400/94‑1 (MQ=25)
          gCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGc                                                                        <  1:8213766/94‑1 (MQ=25)
          gCAGAGCGGATGATTAA‑TGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGGAGCGGTACTGCAACATTCCTTTTGGAACAAGGTAAGCCGGGAGCa                                                                       >  2:5207121/1‑94 (MQ=16)
           cAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCa                                                                       >  1:1555369/1‑94 (MQ=25)
           cAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCa                                                                       >  2:7086837/1‑94 (MQ=25)
           cAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCa                                                                       >  1:553538/1‑94 (MQ=25)
            agagCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAg                                                                      <  1:865509/94‑1 (MQ=25)
            agagCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAg                                                                      >  1:4549946/1‑94 (MQ=25)
               gCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCa                                                                   >  1:9485803/1‑94 (MQ=25)
                cGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCAc                                                                  <  2:10571596/94‑1 (MQ=255)
                 ggATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACg                                                                 >  1:5372511/1‑94 (MQ=255)
                 ggATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACg                                                                 >  1:6477207/1‑94 (MQ=255)
                   atgatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCCGGCACGTg                                                               >  2:3507045/1‑94 (MQ=255)
                   atgatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTg                                                               <  1:3057723/94‑1 (MQ=255)
                    tgatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGa                                                              >  1:4362546/1‑94 (MQ=255)
                     gatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGAc                                                             <  1:457686/94‑1 (MQ=255)
                     gatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGAc                                                             <  2:8095773/94‑1 (MQ=255)
                      atTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACg                                                            >  1:3551601/1‑94 (MQ=255)
                        tAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGAt                                                          >  1:10124258/1‑94 (MQ=255)
                         aaGTGTCTGCCTCGTGAGTCCAGCAGCGCGGTAGTATCGCTACTGCAACATCCCGTTCGAACAACGTTACCCCGGGAGCAGGCACGTGACGAtt                                                         <  2:4760873/94‑1 (MQ=255)
                         aaGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGAtt                                                         >  2:7948968/1‑94 (MQ=255)
                          aGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGAttt                                                        >  2:2438564/1‑94 (MQ=255)
                          aGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGAttt                                                        <  2:2188982/94‑1 (MQ=255)
                            tgtCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCg                                                      <  2:5090943/94‑1 (MQ=255)
                            tgtCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCg                                                      <  2:647087/94‑1 (MQ=255)
                             gtCTGCCGCGTGAGGCCAGCAGCCCGGTAGGAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCgg                                                     <  1:2839774/94‑1 (MQ=255)
                              tCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGt                                                    <  1:9147448/94‑1 (MQ=255)
                               cTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTg                                                   <  1:1529857/94‑1 (MQ=255)
                                tGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGa                                                  >  1:9713726/1‑94 (MQ=255)
                                tGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGa                                                  >  2:14764/1‑94 (MQ=255)
                                tGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGa                                                  >  1:2394503/1‑94 (MQ=255)
                                 gCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGaa                                                 <  1:3251851/94‑1 (MQ=255)
                                  ccGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGaaa                                                <  1:6569600/94‑1 (MQ=255)
                                   cgcgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAAc                                               <  1:6859623/94‑1 (MQ=255)
                                    gcgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAAcc                                              >  2:6697154/1‑94 (MQ=255)
                                    gcgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAAcc                                              >  2:6070171/1‑94 (MQ=255)
                                     cgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCg                                             >  1:3953323/1‑94 (MQ=255)
                                      gTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGt                                            >  1:9371804/1‑94 (MQ=255)
                                       tGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGCAACCGTa                                           >  1:587244/1‑94 (MQ=255)
                                        gAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAg                                          <  1:6329686/94‑1 (MQ=255)
                                        gAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAg                                          <  2:1273662/94‑1 (MQ=255)
                                         aggccAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGATACCGTAgg                                         >  1:1857361/4‑93 (MQ=255)
                                          ggccAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAggt                                        <  1:592008/92‑3 (MQ=255)
                                            ccAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAggtgc                                      <  2:7529628/94‑5 (MQ=255)
                                              agcagcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCa                                    <  2:8426556/94‑1 (MQ=255)
                                               gcagcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCAt                                   <  1:3519566/94‑1 (MQ=255)
                                                 agcCCGGTAGTAGCGCTANTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATNTCGGTGAAACCGTNGGTGCCATAc                                 >  1:1343875/1‑94 (MQ=255)
                                                 agcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAc                                 <  2:5800211/94‑1 (MQ=255)
                                                   cccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGCGCCATACTc                               >  1:10529033/1‑94 (MQ=255)
                                                    ccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCg                              >  1:2981365/1‑94 (MQ=255)
                                                    ccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCg                              >  1:10037909/1‑94 (MQ=255)
                                                    ccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCg                              <  1:6742776/94‑1 (MQ=255)
                                                     cGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGa                             >  2:7619829/1‑94 (MQ=255)
                                                        tagtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAc                          <  2:6891261/94‑1 (MQ=255)
                                                         agtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACg                         >  2:2381540/1‑94 (MQ=255)
                                                         agtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACg                         >  2:8295589/1‑94 (MQ=255)
                                                         agtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACg                         <  1:6915813/94‑1 (MQ=255)
                                                          gtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACgg                        <  1:4672526/94‑1 (MQ=255)
                                                          gtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACgg                        <  2:3478178/94‑1 (MQ=255)
                                                           tagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGACACCGTAGGTGCCATACTCGATACGGt                       >  2:4347737/1‑94 (MQ=255)
                                                           tagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGt                       <  1:7164392/94‑1 (MQ=255)
                                                            agCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTa                      >  2:5584623/1‑94 (MQ=255)
                                                            agCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACACGACACGGTa                      <  1:1082866/94‑1 (MQ=255)
                                                              ctcTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAGCCGTAGGTGCCATACTCGATACGGTAAg                    <  2:7340801/92‑1 (MQ=255)
                                                               gcTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGc                   >  1:9927657/1‑94 (MQ=255)
                                                                cTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCa                  >  2:4416479/1‑94 (MQ=255)
                                                                cTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCa                  <  2:6173608/94‑1 (MQ=255)
                                                                cTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCa                  <  1:806769/94‑1 (MQ=255)
                                                                 tACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAc                 <  1:6134201/94‑1 (MQ=255)
                                                                 tACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAc                 >  1:2631244/1‑94 (MQ=255)
                                                                   cTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCt               >  1:3833849/1‑94 (MQ=255)
                                                                   cTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCt               >  1:6071105/1‑94 (MQ=255)
                                                                   cTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGCAAGCACCt               >  2:7017515/1‑94 (MQ=255)
                                                                    tGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCtt              <  2:10403495/94‑1 (MQ=255)
                                                                        aCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTccc          <  2:1688825/94‑1 (MQ=255)
                                                                         cATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcccc         >  1:6586594/1‑94 (MQ=255)
                                                                          aTCCCTTTCGAACAACGGTAGCCCGGGAGCAGGGACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCt        >  2:9431431/1‑94 (MQ=255)
                                                                          aTCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCt        >  2:4011551/1‑94 (MQ=255)
                                                                          aTCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCt        <  1:4154897/94‑1 (MQ=255)
                                                                           tCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCtt       <  2:10270384/94‑1 (MQ=255)
                                                                            ccccTTCGAACAACGGTTTCCCGGGAGCAGGCACGTGACGATTTCGGTGAATCCGTAGGTGCCATACTCGATACTGTATGCTCTTTTTCCCTTa      >  1:9387635/1‑94 (MQ=255)
                                                                            cccTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTa      >  2:5505672/1‑94 (MQ=255)
                                                                            cccTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTa      <  2:747699/94‑1 (MQ=255)
                                                                            cccTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTa      >  2:9161265/1‑94 (MQ=255)
                                                                              cTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTAtt    <  2:5025066/94‑1 (MQ=255)
                                                                              cTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTAtt    >  2:3555725/1‑94 (MQ=255)
                                                                              cTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTAtt    >  1:7867212/1‑94 (MQ=255)
                                                                              cTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTAtt    >  1:7122567/1‑94 (MQ=255)
                                                                               tttCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTc   <  2:3768863/94‑1 (MQ=255)
                                                                                ttCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTcc  <  1:1990110/94‑1 (MQ=255)
                                                                                        ||                                                                                    
AAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGCCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATTCC  >  NC_000913/2766339‑2766512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGCCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATT  >  NC_000913/2766340‑2766510
                                                                                       |                                                                                   
AGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAG                                                                               <  SRR4302545.4178126/94‑1 (MQ=60)
AGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAG                                                                               >  SRR4302545.7562837/1‑94 (MQ=60)
AGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAG                                                                               >  SRR4302545.3152764/1‑94 (MQ=60)
AGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAG                                                                               <  SRR4302545.1358990/94‑1 (MQ=60)
  GGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGGGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCC                                                                             >  SRR4302545.9733029/1‑94 (MQ=60)
   GTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCC                                                                            >  SRR4302545.2345289/1‑94 (MQ=60)
    TGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCG                                                                           <  SRR4302545.7199632/94‑1 (MQ=60)
    TGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCG                                                                           <  SRR4302545.6742442/94‑1 (MQ=60)
    TGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCG                                                                           >  SRR4302545.949124/1‑94 (MQ=60)
    TGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCG                                                                           >  SRR4302545.4181139/1‑94 (MQ=60)
     GATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGG                                                                          >  SRR4302545.6129325/1‑94 (MQ=60)
       TGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGA                                                                        >  SRR4302545.824133/1‑94 (MQ=60)
       TGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGA                                                                        >  SRR4302545.3084462/1‑94 (MQ=60)
       TGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGA                                                                        <  SRR4302545.7636740/94‑1 (MQ=60)
       TGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGA                                                                        >  SRR4302545.7498836/1‑94 (MQ=60)
        GGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAG                                                                       <  SRR4302545.2486646/94‑1 (MQ=60)
         GCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGC                                                                      <  SRR4302545.8451560/94‑1 (MQ=60)
          CAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCA                                                                     >  SRR4302545.565911/1‑94 (MQ=60)
          CAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCA                                                                     >  SRR4302545.1591953/1‑94 (MQ=60)
          CAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCA                                                                     <  SRR4302545.2452047/94‑1 (MQ=60)
           AGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAG                                                                    <  SRR4302545.884922/94‑1 (MQ=60)
           AGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAG                                                                    >  SRR4302545.4666644/1‑94 (MQ=60)
             AGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGC                                                                  >  SRR4302545.6078179/1‑94 (MQ=60)
             AGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGC                                                                  <  SRR4302545.10081613/94‑1 (MQ=60)
              GCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCA                                                                 <  SRR4302545.6796722/94‑1 (MQ=60)
              GCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCA                                                                 >  SRR4302545.9764947/1‑94 (MQ=60)
              GCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCA                                                                 >  SRR4302545.693114/1‑94 (MQ=60)
               TGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCAC                                                                >  SRR4302545.1687888/1‑94 (MQ=60)
               CGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCAC                                                                <  SRR4302545.3608129/94‑1 (MQ=60)
               CGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGCGAGCAGGCAC                                                                >  SRR4302545.10358105/1‑94 (MQ=60)
                GGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACG                                                               >  SRR4302545.5533915/1‑94 (MQ=60)
                GGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACG                                                               >  SRR4302545.5512230/1‑94 (MQ=60)
                GGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACG                                                               >  SRR4302545.6654288/1‑94 (MQ=60)
                GGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACG                                                               >  SRR4302545.2765988/1‑94 (MQ=60)
                 GATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGT                                                              >  SRR4302545.92472/1‑94 (MQ=60)
                  ATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTG                                                             <  SRR4302545.3132698/94‑1 (MQ=60)
                  ATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTG                                                             >  SRR4302545.7531269/1‑94 (MQ=60)
                   TGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGA                                                            >  SRR4302545.4472810/1‑94 (MQ=60)
                    GATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGAC                                                           <  SRR4302545.467882/94‑1 (MQ=60)
                     ATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACG                                                          >  SRR4302545.3638447/1‑94 (MQ=60)
                     ATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACG                                                          >  SRR4302545.1801806/1‑94 (MQ=60)
                     ATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACG                                                          >  SRR4302545.6234216/1‑94 (MQ=60)
                     ATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACA                                                          <  SRR4302545.3297952/94‑1 (MQ=60)
                       TAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGAT                                                        >  SRR4302545.10423654/1‑94 (MQ=60)
                        AAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATT                                                       >  SRR4302545.4686763/1‑94 (MQ=60)
                        AAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATT                                                       >  SRR4302545.9481852/1‑94 (MQ=60)
                        AAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATT                                                       <  SRR4302545.8761338/94‑1 (MQ=60)
                         AGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTT                                                      <  SRR4302545.43555/94‑1 (MQ=60)
                          GTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTC                                                     <  SRR4302545.10485208/94‑1 (MQ=60)
                          GTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTC                                                     <  SRR4302545.1585762/94‑1 (MQ=60)
                            GTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGG                                                   >  SRR4302545.9930221/1‑94 (MQ=60)
                            GTCTGCCGCGTGAGGCCAGCAGCCCGGTAGGAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGG                                                   <  SRR4302545.2908450/94‑1 (MQ=60)
                             TCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGT                                                  <  SRR4302545.2923911/94‑1 (MQ=60)
                             TCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGT                                                  <  SRR4302545.9416025/94‑1 (MQ=60)
                              CTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTG                                                 <  SRR4302545.5481052/94‑1 (MQ=60)
                              CTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTG                                                 <  SRR4302545.1565633/94‑1 (MQ=60)
                              CTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTG                                                 <  SRR4302545.7450031/94‑1 (MQ=60)
                               TGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAATAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGA                                                >  SRR4302545.4743782/1‑94 (MQ=60)
                               TGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGA                                                >  SRR4302545.10003079/1‑94 (MQ=60)
                               TGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGA                                                >  SRR4302545.2452595/1‑94 (MQ=60)
                                GCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAA                                               >  SRR4302545.9785518/1‑94 (MQ=60)
                                GCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAA                                               >  SRR4302545.7980945/1‑94 (MQ=60)
                                GCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAA                                               <  SRR4302545.3331670/94‑1 (MQ=60)
                                 CCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAA                                              <  SRR4302545.6749362/94‑1 (MQ=60)
                                 CCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAA                                              <  SRR4302545.6749363/94‑1 (MQ=60)
                                  CGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAAC                                             <  SRR4302545.7048082/94‑1 (MQ=60)
                                   GCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACC                                            >  SRR4302545.9066076/1‑94 (MQ=60)
                                    CGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCG                                           <  SRR4302545.1986103/94‑1 (MQ=60)
                                    CGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCG                                           >  SRR4302545.4052109/1‑94 (MQ=60)
                                     GTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGT                                          >  SRR4302545.962716/1‑94 (MQ=60)
                                     GTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGT                                          >  SRR4302545.9646781/1‑94 (MQ=60)
                                     GTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGT                                          >  SRR4302545.599993/1‑94 (MQ=60)
                                      TGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGCAACCGTA                                         >  SRR4302545.600512/1‑94 (MQ=60)
                                       GAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAG                                        >  SRR4302545.6885034/1‑94 (MQ=60)
                                       GAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAG                                        <  SRR4302545.1148320/94‑1 (MQ=60)
                                       GAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAG                                        <  SRR4302545.6502312/94‑1 (MQ=60)
                                        AGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGATACCGTAGG                                       >  SRR4302545.1902775/1‑94 (MQ=60)
                                         GGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGT                                      <  SRR4302545.605373/94‑1 (MQ=60)
                                         GGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGT                                      >  SRR4302545.3130046/1‑94 (MQ=60)
                                         GGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGT                                      <  SRR4302545.10087448/94‑1 (MQ=60)
                                           GCAGCAGCCCTGTAGTAGCGCTACTGCATCATCCCTTACGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGgtgc                                    <  SRR4302545.3403365/94‑5 (MQ=60)
                                           CCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGgtgc                                    <  SRR4302545.4502868/94‑5 (MQ=60)
                                              GCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCAT                                 <  SRR4302545.3605890/94‑1 (MQ=60)
                                               CAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATA                                >  SRR4302545.9647492/1‑94 (MQ=60)
                                               CAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATA                                <  SRR4302545.6624103/94‑1 (MQ=60)
                                               CAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATA                                <  SRR4302545.529452/94‑1 (MQ=60)
                                                AGCCCGGTAGTAGCGCTANTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATNTCGGTGAAACCGTNGGTGCCATAC                               >  SRR4302545.1373845/1‑94 (MQ=60)
                                                AGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGgtgccctac                               >  SRR4302545.6751025/1‑85 (MQ=60)
                                                AGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAC                               <  SRR4302545.6376142/94‑1 (MQ=60)
                                                 GCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACT                              >  SRR4302545.6512997/1‑94 (MQ=60)
                                                  CCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTC                             >  SRR4302545.2652276/1‑94 (MQ=60)
                                                  CCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTC                             >  SRR4302545.8357867/1‑94 (MQ=60)
                                                  CCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGCGCCATACTC                             >  SRR4302545.10839214/1‑94 (MQ=60)
                                                   CCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCG                            >  SRR4302545.3054010/1‑94 (MQ=60)
                                                   CCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCG                            <  SRR4302545.6927640/94‑1 (MQ=60)
                                                   CCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCG                            >  SRR4302545.10335211/1‑94 (MQ=60)
                                                     GGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAT                          >  SRR4302545.731705/1‑94 (MQ=60)
                                                      GTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATA                         >  SRR4302545.7260955/1‑94 (MQ=60)
                                                        AGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATCCTCGATACG                       <  SRR4302545.7106365/94‑1 (MQ=60)
                                                        AGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACG                       <  SRR4302545.3722029/94‑1 (MQ=60)
                                                        AGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACG                       <  SRR4302545.196010/94‑1 (MQ=60)
                                                        AGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACG                       <  SRR4302545.7106364/94‑1 (MQ=60)
                                                         GTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGG                      <  SRR4302545.152128/94‑1 (MQ=60)
                                                         GTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGG                      <  SRR4302545.4791762/94‑1 (MQ=60)
                                                          TAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGT                     <  SRR4302545.7363359/94‑1 (MQ=60)
                                                          TAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGT                     <  SRR4302545.10844835/94‑1 (MQ=60)
                                                          TAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGT                     <  SRR4302545.10844839/94‑1 (MQ=60)
                                                           AGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTA                    <  SRR4302545.7055182/94‑1 (MQ=60)
                                                           AGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACACGACACGGTA                    <  SRR4302545.1106322/94‑1 (MQ=60)
                                                           AGCGCTACTGCAACATCCCTTCCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTA                    <  SRR4302545.7055181/94‑1 (MQ=60)
                                                             CGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAG                  <  SRR4302545.107538/94‑1 (MQ=60)
                                                             CGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAG                  >  SRR4302545.3816458/1‑94 (MQ=60)
                                                             CGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAG                  <  SRR4302545.3943349/94‑1 (MQ=60)
                                                              GCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGC                 >  SRR4302545.10222486/1‑94 (MQ=60)
                                                              GCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGC                 <  SRR4302545.5900990/94‑1 (MQ=60)
                                                              GCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGC                 >  SRR4302545.3548027/1‑94 (MQ=60)
                                                              GCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGA                 >  SRR4302545.8360196/1‑94 (MQ=60)
                                                               CTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCA                <  SRR4302545.824891/94‑1 (MQ=60)
                                                                TACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAC               <  SRR4302545.6300996/94‑1 (MQ=60)
                                                                TACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAC               <  SRR4302545.6300995/94‑1 (MQ=60)
                                                                TACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAC               >  SRR4302545.2694574/1‑94 (MQ=60)
                                                                 ACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCTATACGGTAAGCACC              >  SRR4302545.1399025/1‑94 (MQ=60)
                                                                 ACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACC              <  SRR4302545.3337112/94‑1 (MQ=60)
                                                                  CTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCT             >  SRR4302545.3929432/1‑94 (MQ=60)
                                                                  CTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCT             >  SRR4302545.6235896/1‑94 (MQ=60)
                                                                  CTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCT             >  SRR4302545.3929431/1‑94 (MQ=60)
                                                                    GCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTT           <  SRR4302545.4665337/94‑1 (MQ=60)
                                                                    GCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTT           >  SRR4302545.627308/1‑94 (MQ=60)
                                                                    GCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTT           >  SRR4302545.7022235/1‑94 (MQ=60)
                                                                     CAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTC          <  SRR4302545.8055340/94‑1 (MQ=60)
                                                                     CAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTC          >  SRR4302545.7402533/1‑94 (MQ=60)
                                                                     CAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTC          <  SRR4302545.6288178/94‑1 (MQ=60)
                                                                      AACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAGCTTTCC         >  SRR4302545.5529207/1‑94 (MQ=60)
                                                                      AACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCC         <  SRR4302545.4752776/94‑1 (MQ=60)
                                                                       ACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCC        <  SRR4302545.8620895/94‑1 (MQ=60)
                                                                        CATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCC       <  SRR4302545.2937950/94‑1 (MQ=60)
                                                                        CATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCC       >  SRR4302545.6766861/1‑94 (MQ=60)
                                                                         ATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCT      <  SRR4302545.4258633/94‑1 (MQ=60)
                                                                          TCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTT     <  SRR4302545.7359527/94‑1 (MQ=60)
                                                                             CTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATT  >  SRR4302545.8147380/1‑94 (MQ=60)
                                                                             CTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATT  >  SRR4302545.7320000/1‑94 (MQ=60)
                                                                             CTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATT  >  SRR4302545.8090400/1‑94 (MQ=60)
                                                                             CTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATT  >  SRR4302545.8090401/1‑94 (MQ=60)
                                                                                       |                                                                                   
AGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGCCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATT  >  NC_000913/2766340‑2766510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑