Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,821,415 T→C A126A (GCA→GCG)  recA ← RecA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,821,4150TC98.8% 1456.0 / ‑0.1 429A126A (GCA→GCG) recARecA
Reads supporting (aligned to +/- strand):  ref base T (4/1);  new base C (205/218);  total (210/219)
Fisher's exact test for biased strand distribution p-value = 2.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 443 total aligned reads displayed.

CGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCA  >  NC_000913/2821324‑2821505
                                                                                           |                                                                                          
 gATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGcc                                                                                         >  1:6671261/1‑94 (MQ=255)
     tCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCt                                                                                     <  1:5669462/94‑1 (MQ=255)
     tCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCt                                                                                     <  2:2373227/94‑1 (MQ=255)
      ccGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTc                                                                                    <  1:3764043/94‑1 (MQ=255)
      ccGCTCTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCAAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTc                                                                                    <  1:1094725/94‑1 (MQ=255)
        gCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGc                                                                                  >  1:221228/1‑94 (MQ=255)
         cTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCATGGCGTCACAGATTTCCACGCCCGGCTCGcg                                                                                 >  1:6251976/1‑93 (MQ=255)
         cTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGcc                                                                                 >  2:7054008/1‑94 (MQ=255)
         cTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGcc                                                                                 <  1:9299220/94‑1 (MQ=255)
           tgcGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCgg                                                                               <  2:4300982/92‑1 (MQ=255)
            tCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGgt                                                                              <  2:9201774/94‑1 (MQ=255)
            tCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGgt                                                                              <  2:7462072/94‑1 (MQ=255)
              ggCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGgtgt                                                                            <  1:3609906/94‑1 (MQ=255)
               gCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTc                                                                           <  1:2304744/94‑1 (MQ=255)
                cggcAGGGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTcc                                                                          <  1:8378980/91‑1 (MQ=255)
                cGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTcc                                                                          <  1:3030714/94‑1 (MQ=255)
                 gTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCg                                                                         <  1:1007374/94‑1 (MQ=255)
                 gTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCg                                                                         >  2:3769327/1‑94 (MQ=255)
                    aGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCt                                                                      <  2:1608661/94‑1 (MQ=255)
                     gTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTg                                                                     <  1:10013290/94‑1 (MQ=255)
                      tGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGACACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTgg                                                                    <  1:6482808/94‑1 (MQ=255)
                       gccgccACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTggg                                                                   >  1:3995034/1‑94 (MQ=255)
                            cACGGAGTCACCGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGcac                                                              <  2:3149078/94‑1 (MQ=255)
                            cACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGTTGTCCGGCTGGGAGcac                                                              <  1:4178192/94‑1 (MQ=255)
                            cACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGcac                                                              >  1:2666592/1‑94 (MQ=255)
                            cACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGcac                                                              >  2:6447987/1‑94 (MQ=255)
                             aCGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGcaca                                                             >  1:94609/1‑94 (MQ=255)
                                 aGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCAcagcag                                                         >  2:5021259/1‑94 (MQ=255)
                                 aGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCAcagcag                                                         >  2:9861519/1‑94 (MQ=255)
                                 aGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCAcagcag                                                         >  1:5477474/1‑94 (MQ=255)
                                  gTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAgg                                                        >  2:5794891/1‑94 (MQ=255)
                                     aaCGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTg                                                     >  2:6309244/1‑94 (MQ=255)
                                     aaCGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTg                                                     <  1:485256/94‑1 (MQ=255)
                                     aaCGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTg                                                     >  2:304873/1‑94 (MQ=255)
                                       cgacgaTAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTc                                                   <  2:4659253/94‑1 (MQ=255)
                                       cgacgaTAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTc                                                   >  2:10204614/1‑94 (MQ=255)
                                         acgaTAACGTCTACTGCGCCAGAACGCGCCAGGGGGCCACAGATTTCCAGCGCCTGCTCGCCGGGGTCCGGCTGGGGGCACAGCAGGTTGtaca                                                 >  2:2756279/1‑91 (MQ=255)
                                         acgaTAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGTCTGGGAGCACAGCAGGTTGTCGa                                                 >  1:5867965/1‑94 (MQ=255)
                                         acgaTAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGa                                                 >  1:1723346/1‑94 (MQ=255)
                                         acgaTAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGa                                                 <  1:4602263/94‑1 (MQ=255)
                                          cgaTAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGat                                                >  2:9641505/1‑94 (MQ=255)
                                            aTAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGatat                                              >  2:14220/1‑94 (MQ=255)
                                             tAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATc                                             <  2:8925896/94‑1 (MQ=255)
                                             tAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATc                                             <  2:7266627/94‑1 (MQ=255)
                                              aaCGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCg                                            >  1:8711701/1‑94 (MQ=255)
                                              aaCGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCg                                            <  2:6030759/94‑1 (MQ=255)
                                              aaCGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCg                                            <  2:10050449/94‑1 (MQ=255)
                                               aCGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGa                                           <  1:1978553/94‑1 (MQ=255)
                                                cGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGAc                                          >  2:618055/1‑94 (MQ=255)
                                                cGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATATCCAGCGCCTGCTCGCCGGCGTCCGGCTGGGAGCACAGCAGGCTGTCGATATCGAc                                          >  2:6875557/1‑94 (MQ=255)
                                                 gTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATAacgacg                                         >  1:8081970/1‑94 (MQ=255)
                                                 gTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGAcc                                         >  1:10475282/1‑93 (MQ=255)
                                                 gTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACg                                         <  1:1616531/94‑1 (MQ=255)
                                                   cTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCgccgcc                                       >  1:2897363/1‑94 (MQ=255)
                                                   cTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGcc                                       <  2:8038067/94‑1 (MQ=255)
                                                     aCTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGTGAGCACAGCAGGTTGTCGATATCGACGCCCa                                     <  1:4339346/94‑1 (MQ=255)
                                                     aCTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCa                                     <  1:677326/94‑1 (MQ=255)
                                                     aCTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCa                                     >  2:3607409/1‑94 (MQ=255)
                                                       tGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGGCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGt                                   <  1:7863991/94‑1 (MQ=255)
                                                         cgcCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGttt                                 <  1:1221061/94‑1 (MQ=255)
                                                         cgcCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGttt                                 <  2:1966010/94‑1 (MQ=255)
                                                           ccAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTAc                               <  2:8983792/94‑1 (MQ=255)
                                                            cAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACg                              >  2:6603451/1‑94 (MQ=255)
                                                            cAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACg                              >  2:5508390/1‑94 (MQ=255)
                                                            cAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACg                              >  1:6811089/1‑94 (MQ=255)
                                                            cAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACg                              <  2:1193389/94‑1 (MQ=255)
                                                             aGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACgg                             >  1:5098299/1‑93 (MQ=255)
                                                              gAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTg                            <  2:8651919/94‑1 (MQ=255)
                                                              gAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTg                            <  2:7793839/94‑1 (MQ=255)
                                                               aaCGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGc                           >  2:3867579/1‑94 (MQ=255)
                                                                 cgcgCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGt                         <  2:2914055/94‑1 (MQ=255)
                                                                 cgcgCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGt                         <  2:4906662/94‑1 (MQ=255)
                                                                   cgcCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAg                       >  1:3837881/1‑94 (MQ=255)
                                                                   cgcCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAg                       >  2:10553552/1‑94 (MQ=255)
                                                                   cgcCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAg                       <  1:817023/94‑1 (MQ=255)
                                                                       aGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTg                   <  1:445458/94‑1 (MQ=255)
                                                                       aGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTg                   <  1:1911668/94‑1 (MQ=255)
                                                                       aGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTg                   <  1:1411069/94‑1 (MQ=255)
                                                                         ggCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTggg                 <  2:3446059/94‑1 (MQ=255)
                                                                         ggCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTggg                 <  2:9331121/94‑1 (MQ=255)
                                                                          gCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGt                <  1:3274598/94‑1 (MQ=255)
                                                                          gCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGt                >  1:891607/1‑94 (MQ=255)
                                                                           cGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTc               >  2:1779916/1‑94 (MQ=255)
                                                                           cGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTc               <  1:1812458/94‑1 (MQ=255)
                                                                           cGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTc               <  2:849428/94‑1 (MQ=255)
                                                                            gTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTcc              >  2:5223596/1‑94 (MQ=255)
                                                                              cacaGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAg            <  1:9894627/94‑1 (MQ=255)
                                                                              cacaGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAg            >  2:2507440/1‑94 (MQ=255)
                                                                              cacaGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAg            <  2:4406592/94‑1 (MQ=255)
                                                                                caGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAgcg          >  1:5383380/1‑94 (MQ=255)
                                                                                caGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAgcg          >  2:8229679/1‑94 (MQ=255)
                                                                                  gATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGcgcg        <  2:4264362/94‑1 (MQ=255)
                                                                                   aTTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGcgcgg       >  2:214007/1‑93 (MQ=255)
                                                                                   aTTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCgt       >  1:7660914/1‑94 (MQ=255)
                                                                                    tttCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCgtg      <  1:10198331/94‑1 (MQ=255)
                                                                                     ttCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCgtgt     >  1:7289680/1‑94 (MQ=255)
                                                                                      tCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGTAGGTTGTCGATTCTGTCGCCCAGTTTACGTGCGTAGAGTGGGGCCATCGCGTGtt    >  1:9410149/1‑94 (MQ=255)
                                                                                      tCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGtt    >  1:7086534/1‑94 (MQ=255)
                                                                                       ccAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTc   >  2:6690901/1‑94 (MQ=255)
                                                                                        cAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCa  <  1:5525903/94‑1 (MQ=255)
                                                                                           |                                                                                          
CGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCA  >  NC_000913/2821324‑2821505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 238 total aligned reads displayed.
TTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCAT  >  NC_000913/2821322‑2821509
                                                                                             |                                                                                              
TTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGC                                                                                                >  SRR4302545.10829507/1‑94 (MQ=60)
 TCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCG                                                                                               <  SRR4302545.7263803/94‑1 (MQ=60)
  CGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGC                                                                                              >  SRR4302545.1123810/1‑94 (MQ=60)
  CGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGC                                                                                              >  SRR4302545.1123811/1‑94 (MQ=60)
  CGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGC                                                                                              >  SRR4302545.4589431/1‑94 (MQ=60)
   GATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCC                                                                                             >  SRR4302545.6854187/1‑94 (MQ=60)
    ATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCT                                                                                            >  SRR4302545.43505/1‑94 (MQ=60)
    ATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCT                                                                                            >  SRR4302545.2210852/1‑94 (MQ=60)
    ATTTCCGCTTTCGGCGTCAGAGCAGCCACGGAGCCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCT                                                                                            >  SRR4302545.5650796/1‑94 (MQ=60)
     TTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTG                                                                                           <  SRR4302545.10416134/94‑1 (MQ=60)
     TTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTG                                                                                           >  SRR4302545.4035803/1‑94 (MQ=60)
     TTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTG                                                                                           <  SRR4302545.3921356/94‑1 (MQ=60)
     TTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTG                                                                                           <  SRR4302545.1727367/94‑1 (MQ=60)
       TCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCT                                                                                         <  SRR4302545.5820795/94‑1 (MQ=60)
       TCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCT                                                                                         <  SRR4302545.2866050/94‑1 (MQ=60)
        CCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTC                                                                                        <  SRR4302545.3856927/94‑1 (MQ=60)
        CCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTC                                                                                        <  SRR4302545.3838925/94‑1 (MQ=60)
        CCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTC                                                                                        <  SRR4302545.6558384/94‑1 (MQ=60)
        CCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTC                                                                                        >  SRR4302545.10189903/1‑94 (MQ=60)
        CCGCTCTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCAAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTC                                                                                        <  SRR4302545.1118483/94‑1 (MQ=60)
         CGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCG                                                                                       >  SRR4302545.3710004/1‑94 (MQ=60)
          GCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGC                                                                                      >  SRR4302545.225817/1‑94 (MQ=60)
          GCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGC                                                                                      >  SRR4302545.5107659/1‑94 (MQ=60)
           CTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCC                                                                                     <  SRR4302545.9571850/94‑1 (MQ=60)
           CTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCC                                                                                     >  SRR4302545.6422388/1‑94 (MQ=60)
           CTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCC                                                                                     <  SRR4302545.9571849/94‑1 (MQ=60)
            TTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCATGGCGTCACAGATTTCCAGCGCCTGCTCGCCG                                                                                    <  SRR4302545.2208315/94‑1 (MQ=60)
            TTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCG                                                                                    <  SRR4302545.1817885/94‑1 (MQ=60)
             TGCGGCGTAAGTGCGGCCACGGAGTCAATGTCGATAACGTCTACTGCGCCAGACCGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTAACCGG                                                                                   <  SRR4302545.4316645/94‑1 (MQ=60)
               CGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTG                                                                                 <  SRR4302545.176949/94‑1 (MQ=60)
                GGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGT                                                                                <  SRR4302545.4500313/94‑1 (MQ=60)
                GGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGT                                                                                >  SRR4302545.6981171/1‑94 (MQ=60)
                GGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGT                                                                                >  SRR4302545.6821125/1‑94 (MQ=60)
                GGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGT                                                                                <  SRR4302545.3697905/94‑1 (MQ=60)
                GGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGT                                                                                >  SRR4302545.1294448/1‑94 (MQ=60)
                GGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGT                                                                                >  SRR4302545.485481/1‑94 (MQ=60)
                 GCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTC                                                                               <  SRR4302545.5459763/94‑1 (MQ=60)
                 GCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTC                                                                               <  SRR4302545.2360877/94‑1 (MQ=60)
                  CGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCC                                                                              <  SRR4302545.3104901/94‑1 (MQ=60)
                  CGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCC                                                                              >  SRR4302545.10588716/1‑94 (MQ=60)
                  CGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCC                                                                              >  SRR4302545.9414137/1‑94 (MQ=60)
                  CGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCC                                                                              >  SRR4302545.10588717/1‑94 (MQ=60)
                  CGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCC                                                                              <  SRR4302545.1890111/94‑1 (MQ=60)
                  CGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCC                                                                              <  SRR4302545.1890112/94‑1 (MQ=60)
                  CGGCAGGGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCC                                                                              <  SRR4302545.8621563/94‑1 (MQ=60)
                   GTCAGTGCCGCCACGGAGTGAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTACG                                                                             >  SRR4302545.8796414/1‑94 (MQ=60)
                   GTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCG                                                                             <  SRR4302545.8622355/94‑1 (MQ=60)
                   GTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCG                                                                             >  SRR4302545.6539179/1‑94 (MQ=60)
                   GTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCG                                                                             <  SRR4302545.1029651/94‑1 (MQ=60)
                   GTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCG                                                                             <  SRR4302545.966796/94‑1 (MQ=60)
                   GTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCG                                                                             >  SRR4302545.7819144/1‑94 (MQ=60)
                   GTCAGTGCCGCCACGGAATCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCG                                                                             <  SRR4302545.1288167/94‑1 (MQ=60)
                      AGTGCCGCCACAGAGTCAACGACGATAACGTTTGCTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGaagaccggca                                                                          <  SRR4302545.9636833/94‑11 (MQ=60)
                       GTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTG                                                                         <  SRR4302545.10858884/94‑1 (MQ=60)
                       GTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTG                                                                         <  SRR4302545.10310093/94‑1 (MQ=60)
                       GTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTG                                                                         <  SRR4302545.10310092/94‑1 (MQ=60)
                        TGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGG                                                                        <  SRR4302545.6660072/94‑1 (MQ=60)
                         GCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGG                                                                       >  SRR4302545.4094950/1‑94 (MQ=60)
                         GCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGG                                                                       <  SRR4302545.4039882/94‑1 (MQ=60)
                           CGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGTTCGCCCGTGTCCGGCTGGGGG                                                                     >  SRR4302545.9915049/1‑94 (MQ=60)
                            GCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGC                                                                    >  SRR4302545.4040338/1‑94 (MQ=60)
                              CACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGTTGTCCGGCTGGGAGCAC                                                                  <  SRR4302545.4282490/94‑1 (MQ=60)
                              CACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCAC                                                                  >  SRR4302545.2730841/1‑94 (MQ=60)
                               ACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACA                                                                 >  SRR4302545.96795/1‑94 (MQ=60)
                                CGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGGTGGGAGGACAG                                                                >  SRR4302545.8166524/1‑94 (MQ=60)
                                 GGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGC                                                               >  SRR4302545.4388783/1‑94 (MQ=60)
                                 GGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGC                                                               >  SRR4302545.7217182/1‑94 (MQ=60)
                                 GGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGGGTCCGGCTGGGAGaaacgc                                                               >  SRR4302545.10562067/1‑88 (MQ=60)
                                  GAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCA                                                              >  SRR4302545.6406349/1‑94 (MQ=60)
                                   AGTCAACGACGATCACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCAGCTGGGAGCACAGCAG                                                             >  SRR4302545.5621345/1‑94 (MQ=60)
                                   AGTCAACGACGATAACGTCTGCTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAG                                                             <  SRR4302545.7857153/94‑1 (MQ=60)
                                   AGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAG                                                             <  SRR4302545.8968335/94‑1 (MQ=60)
                                   AGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAG                                                             >  SRR4302545.6856184/1‑94 (MQ=60)
                                   AGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAG                                                             <  SRR4302545.3081361/94‑1 (MQ=60)
                                   AGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAG                                                             >  SRR4302545.5621346/1‑94 (MQ=60)
                                   AGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAG                                                             <  SRR4302545.8968336/94‑1 (MQ=60)
                                    GTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGG                                                            >  SRR4302545.7903114/1‑94 (MQ=60)
                                    GTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGG                                                            >  SRR4302545.2592548/1‑94 (MQ=60)
                                      CAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTT                                                          >  SRR4302545.4673728/1‑94 (MQ=60)
                                      CAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTT                                                          <  SRR4302545.3457608/94‑1 (MQ=60)
                                      CAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTT                                                          <  SRR4302545.3277780/94‑1 (MQ=60)
                                      CAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTT                                                          >  SRR4302545.7362321/1‑94 (MQ=60)
                                      CAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTACGGCTGGGAGCACAGCAGGTT                                                          >  SRR4302545.1746687/1‑94 (MQ=60)
                                       AACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTG                                                         >  SRR4302545.6963294/1‑94 (MQ=60)
                                       AACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTG                                                         >  SRR4302545.1326219/1‑94 (MQ=60)
                                       AACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTG                                                         >  SRR4302545.2389385/1‑94 (MQ=60)
                                       AACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTG                                                         <  SRR4302545.496076/94‑1 (MQ=60)
                                       AACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTG                                                         >  SRR4302545.6687986/1‑94 (MQ=60)
                                       AACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTG                                                         <  SRR4302545.10847453/94‑1 (MQ=60)
                                        ACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGT                                                        >  SRR4302545.1582394/1‑94 (MQ=60)
                                         CGACGATAACGTCTCCTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTC                                                       <  SRR4302545.8537368/94‑1 (MQ=60)
                                         CGACGATAACGTCTACTGCGCCAGATCGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTC                                                       <  SRR4302545.5617098/94‑1 (MQ=60)
                                         CGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTC                                                       <  SRR4302545.7629565/94‑1 (MQ=60)
                                         CGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTC                                                       <  SRR4302545.10389070/94‑1 (MQ=60)
                                          GACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCG                                                      <  SRR4302545.2281482/94‑1 (MQ=60)
                                          GACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCG                                                      <  SRR4302545.2347880/94‑1 (MQ=60)
                                          GACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCG                                                      >  SRR4302545.3602906/1‑94 (MQ=60)
                                           ACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGGGCCTTCTCGCCGGTTTCCGTCTGGGAGCAGAGCAGGTTGTCGA                                                     >  SRR4302545.6026061/1‑94 (MQ=60)
                                           ACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGTCTGGGAGCACAGCAGGTTGTCGA                                                     >  SRR4302545.6026062/1‑94 (MQ=60)
                                           ACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGA                                                     <  SRR4302545.4720061/94‑1 (MQ=60)
                                           ACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGA                                                     >  SRR4302545.1764399/1‑94 (MQ=60)
                                           ACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGA                                                     >  SRR4302545.1764398/1‑94 (MQ=60)
                                            CGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGAT                                                    <  SRR4302545.5469726/94‑1 (MQ=60)
                                            CGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGCTTGTCGAT                                                    >  SRR4302545.3285076/1‑94 (MQ=60)
                                             GATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATA                                                   >  SRR4302545.3062578/1‑94 (MQ=60)
                                              ATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATAT                                                  <  SRR4302545.1947159/94‑1 (MQ=60)
                                              ATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATAT                                                  >  SRR4302545.412727/1‑94 (MQ=60)
                                              ATAACGGCTACTTCGCCAGAACGCGCCGGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATAT                                                  <  SRR4302545.537626/94‑1 (MQ=60)
                                               TAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATC                                                 <  SRR4302545.9299640/94‑1 (MQ=60)
                                                AACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCG                                                >  SRR4302545.8964453/1‑94 (MQ=60)
                                                AACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCG                                                >  SRR4302545.2002379/1‑94 (MQ=60)
                                                AACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCG                                                <  SRR4302545.716308/94‑1 (MQ=60)
                                                AACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCG                                                <  SRR4302545.1650265/94‑1 (MQ=60)
                                                AACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCGGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCG                                                <  SRR4302545.9308372/94‑1 (MQ=60)
                                                 ACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGA                                               >  SRR4302545.5342901/1‑94 (MQ=60)
                                                 ACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGA                                               <  SRR4302545.2027557/94‑1 (MQ=60)
                                                  CGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGAC                                              <  SRR4302545.5797121/94‑1 (MQ=60)
                                                   GTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACG                                             >  SRR4302545.8314157/1‑94 (MQ=60)
                                                   GTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACG                                             <  SRR4302545.1654927/94‑1 (MQ=60)
                                                   GTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACG                                             <  SRR4302545.7279121/94‑1 (MQ=60)
                                                   GTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACG                                             >  SRR4302545.7400426/1‑94 (MQ=60)
                                                   GTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACG                                             <  SRR4302545.8649014/94‑1 (MQ=60)
                                                   GTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACC                                             >  SRR4302545.10784110/1‑94 (MQ=60)
                                                   GTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATAACGACG                                             >  SRR4302545.8314156/1‑94 (MQ=60)
                                                    TCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGC                                            >  SRR4302545.238500/1‑94 (MQ=60)
                                                     CTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGCCGCC                                           >  SRR4302545.2967586/1‑94 (MQ=60)
                                                     CTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCC                                           >  SRR4302545.4480823/1‑94 (MQ=60)
                                                       ACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCA                                         <  SRR4302545.692410/94‑1 (MQ=60)
                                                       ACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCA                                         >  SRR4302545.9010374/1‑94 (MQ=60)
                                                        CTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAG                                        <  SRR4302545.7331074/94‑1 (MQ=60)
                                                         TGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGT                                       <  SRR4302545.8087045/94‑1 (MQ=60)
                                                         TGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGT                                       >  SRR4302545.8243223/1‑94 (MQ=60)
                                                         TGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGGCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGT                                       <  SRR4302545.8087044/94‑1 (MQ=60)
                                                           CGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTT                                     <  SRR4302545.7892077/94‑1 (MQ=60)
                                                           CGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTT                                     <  SRR4302545.5417185/94‑1 (MQ=60)
                                                           CGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTT                                     <  SRR4302545.1248760/94‑1 (MQ=60)
                                                           CGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGATGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTT                                     >  SRR4302545.1569727/1‑94 (MQ=60)
                                                            GCCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTA                                    >  SRR4302545.6088621/1‑94 (MQ=60)
                                                             CCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTAC                                   >  SRR4302545.1971026/1‑94 (MQ=60)
                                                             CCAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTAC                                   <  SRR4302545.4684135/94‑1 (MQ=60)
                                                              CAGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACG                                  >  SRR4302545.6998265/1‑94 (MQ=60)
                                                               AGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCCGTTTACGT                                 >  SRR4302545.9915251/1‑94 (MQ=60)
                                                               AGAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGT                                 >  SRR4302545.1241860/1‑94 (MQ=60)
                                                                GAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTG                                <  SRR4302545.386046/94‑1 (MQ=60)
                                                                GAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTG                                >  SRR4302545.6903885/1‑94 (MQ=60)
                                                                GAACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTG                                <  SRR4302545.4233407/94‑1 (MQ=60)
                                                                 AGCGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCTCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGC                               <  SRR4302545.9735069/94‑1 (MQ=60)
                                                                 AACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGC                               <  SRR4302545.9735068/94‑1 (MQ=60)
                                                                  ACGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTGGTCGATATCGACGCCCAGTTTACGTGGG                              >  SRR4302545.8344951/1‑94 (MQ=60)
                                                                  ACGCGCCAGGGCGTCACAGATTCCCCGCGCCTGCTCGCCGGGGCCGGGCGGGGAGCACAGGGGGTTGTCGATATCGACGCCCAGTTTACGTGCG                              <  SRR4302545.7316032/94‑1 (MQ=53)
                                                                   CGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGT                             >  SRR4302545.10320156/1‑94 (MQ=60)
                                                                   CGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGT                             <  SRR4302545.8632799/94‑1 (MQ=60)
                                                                   CGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGT                             <  SRR4302545.1966721/94‑1 (MQ=60)
                                                                   CGCGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGT                             <  SRR4302545.5718288/94‑1 (MQ=60)
                                                                     CGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAG                           <  SRR4302545.835339/94‑1 (MQ=60)
                                                                     CGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAG                           >  SRR4302545.3933588/1‑94 (MQ=60)
                                                                     CGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAG                           <  SRR4302545.1615058/94‑1 (MQ=60)
                                                                     CGCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAG                           >  SRR4302545.3875902/1‑94 (MQ=60)
                                                                      GCCAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACATGCGTAGA                          >  SRR4302545.5700044/1‑94 (MQ=60)
                                                                        CAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATT                        >  SRR4302545.219737/1‑94 (MQ=60)
                                                                        CAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATT                        <  SRR4302545.952634/94‑1 (MQ=60)
                                                                        CAGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATAT‑GACGCCCAGTTTACGTGCGTAGATTG                       <  SRR4302545.455347/94‑1 (MQ=60)
                                                                         AGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTG                       <  SRR4302545.1958917/94‑1 (MQ=60)
                                                                         AGGGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTG                       <  SRR4302545.455348/94‑1 (MQ=60)
                                                                           GGCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGCCGCCCAGTTTACGTGCGTAGATTGGG                     <  SRR4302545.1880612/94‑1 (MQ=60)
                                                                            GCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGTGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGT                    <  SRR4302545.6846099/94‑1 (MQ=60)
                                                                            GCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGT                    <  SRR4302545.5962540/94‑1 (MQ=60)
                                                                            GCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGT                    <  SRR4302545.3354859/94‑1 (MQ=60)
                                                                            GCGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGT                    >  SRR4302545.911596/1‑94 (MQ=60)
                                                                             CGTCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTC                   <  SRR4302545.1856352/94‑1 (MQ=60)
                                                                              GGCACAGATTTCCAGCGCATGCTCACCGGTGTCCGGCTGAGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCC                  <  SRR4302545.10499156/94‑1 (MQ=60)
                                                                               TCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCA                 >  SRR4302545.7119339/1‑94 (MQ=60)
                                                                               TCACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCA                 >  SRR4302545.10510539/1‑94 (MQ=60)
                                                                                CACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAG                >  SRR4302545.2698741/1‑94 (MQ=60)
                                                                                CACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAG                <  SRR4302545.10188597/94‑1 (MQ=60)
                                                                                 ACAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGC               <  SRR4302545.1095676/94‑1 (MQ=60)
                                                                                  CAGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCG              <  SRR4302545.3319594/94‑1 (MQ=60)
                                                                                   AGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGC             >  SRR4302545.2795730/1‑94 (MQ=60)
                                                                                   AGATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGC             >  SRR4302545.2872840/1‑94 (MQ=60)
                                                                                    GATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCG            <  SRR4302545.6653982/94‑1 (MQ=60)
                                                                                     ATTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGT           >  SRR4302545.7877725/1‑94 (MQ=60)
                                                                                      TTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTG          <  SRR4302545.5935833/94‑1 (MQ=60)
                                                                                      TTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTG          >  SRR4302545.373081/1‑94 (MQ=60)
                                                                                      TTTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTG          <  SRR4302545.10499610/94‑1 (MQ=60)
                                                                                       TTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGT         >  SRR4302545.7494121/1‑94 (MQ=60)
                                                                                       TTCCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGT         >  SRR4302545.493247/1‑94 (MQ=60)
                                                                                         CCAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTC       <  SRR4302545.8562294/94‑1 (MQ=60)
                                                                                          CAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCA      <  SRR4302545.8206990/94‑1 (MQ=60)
                                                                                          CAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCA      <  SRR4302545.4869367/94‑1 (MQ=60)
                                                                                          CAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCA      <  SRR4302545.5137851/94‑1 (MQ=60)
                                                                                          CAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCA      >  SRR4302545.5591367/1‑94 (MQ=60)
                                                                                          CAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCA      >  SRR4302545.5591369/1‑94 (MQ=60)
                                                                                          CAGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCA      <  SRR4302545.5671747/94‑1 (MQ=60)
                                                                                          CAGCGCCTGCTCGCCGGTGTCCGGCTGCGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCA      <  SRR4302545.5137850/94‑1 (MQ=60)
                                                                                           AGCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAG     >  SRR4302545.9407637/1‑94 (MQ=60)
                                                                                            GCGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGC    <  SRR4302545.9947031/94‑1 (MQ=60)
                                                                                             CGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCA   <  SRR4302545.3963867/94‑1 (MQ=60)
                                                                                             CGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCA   >  SRR4302545.2987182/1‑94 (MQ=60)
                                                                                             CGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCA   <  SRR4302545.2385791/94‑1 (MQ=60)
                                                                                             CGCCTGCTCGCCGGTGTCCGGCT‑GGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCAT  >  SRR4302545.4468768/1‑94 (MQ=60)
                                                                                             |                                                                                              
TTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCAT  >  NC_000913/2821322‑2821509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑