Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,838,044 +G coding (498/1458 nt) ascF → PTS system enzyme IIBC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,838,0431.G99.7% 1158.0 / ‑3.9 332coding (497/1458 nt)ascFPTS system enzyme IIBC
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (141/190);  total (141/191)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.35e-01

Only 100 of 378 total aligned reads displayed.

AGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑C‑GCATCTGCCGCGCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGA  >  NC_000913/2837950‑2838132
                                                                                               |                                                                                         
agaTGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcg‑c                                                                                            >  2:1944126/1‑94 (MQ=255)
 gaTGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcg‑c‑g                                                                                          <  2:655427/94‑1 (MQ=255)
  aTGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcg‑c‑gg                                                                                         >  1:3078189/1‑93 (MQ=255)
   tGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcg‑c‑ggc                                                                                        >  1:2899845/1‑92 (MQ=255)
    gAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcg‑c‑ggca                                                                                       >  1:1057649/1‑91 (MQ=255)
    gAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcg‑c‑ggca                                                                                       <  1:4131428/94‑4 (MQ=255)
     aGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTcg‑c‑ggcat                                                                                      >  2:6978195/1‑90 (MQ=255)
       cggcgTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCt                                                                                     <  2:1729442/94‑1 (MQ=255)
         gcgTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGc                                                                                   <  1:7284532/94‑1 (MQ=255)
          cgTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGcc                                                                                  <  1:148993/94‑1 (MQ=255)
           gTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCCTCTGCcg                                                                                 >  2:2255484/1‑94 (MQ=255)
           gTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCcg                                                                                 <  1:2484388/94‑1 (MQ=255)
           gTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGCGATTGGTGACGCTGCTTTCTTCTTCCTGCCGCTGATGGTTGAG‑GCATCTGctc                                                                                 >  2:2713187/1‑92 (MQ=255)
               tGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCcgccat                                                                             <  1:7541132/94‑4 (MQ=255)
               tGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCcgccat                                                                             >  2:8828483/1‑91 (MQ=255)
                gACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCcgccatc                                                                            >  2:1359450/1‑90 (MQ=255)
                gACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCcgccatc                                                                            >  2:10095904/1‑90 (MQ=255)
                 aCAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCa                                                                          <  2:1222763/94‑1 (MQ=255)
                 aCAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCa                                                                          >  1:4683400/1‑94 (MQ=255)
                  cAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCaa                                                                         >  1:5130390/1‑94 (MQ=255)
                  cAAAAGGATCGCCGACCTTAACCATTCTGAATGAGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCaa                                                                         <  2:10195310/94‑1 (MQ=255)
                    aaaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAAt                                                                       >  1:2346722/1‑94 (MQ=255)
                    aaaGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAAt                                                                       <  1:4979571/94‑1 (MQ=255)
                      aGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAAttt                                                                     >  1:968468/1‑94 (MQ=255)
                      aGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAAttt                                                                     >  2:9770425/1‑94 (MQ=255)
                       ggATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTa                                                                    >  2:410018/1‑94 (MQ=255)
                       ggATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTa                                                                    <  2:6168049/94‑1 (MQ=255)
                        gATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTaa                                                                   <  1:4803287/94‑1 (MQ=255)
                        gATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTaa                                                                   >  2:2396356/1‑94 (MQ=255)
                         aTCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTaaa                                                                  <  2:7474199/94‑1 (MQ=255)
                            gCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAAcc                                                               <  2:4262637/94‑1 (MQ=255)
                             ccGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCa                                                              >  1:8521740/1‑94 (MQ=255)
                             ccGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCa                                                              >  2:8551203/1‑94 (MQ=255)
                              cGACCTTAACCATTCTGAATGTGATTGGTGAGGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCaa                                                             >  2:4689045/1‑94 (MQ=255)
                              cGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCaa                                                             >  1:1799492/1‑94 (MQ=255)
                               gACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAat                                                            >  1:5495328/1‑94 (MQ=255)
                               gACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAat                                                            >  1:3782830/1‑94 (MQ=255)
                                aCCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAata                                                           <  1:7180096/94‑1 (MQ=255)
                                 ccTTACCCACTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAatat                                                          <  1:4366515/94‑1 (MQ=255)
                                 ccTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAatat                                                          <  2:2972029/94‑1 (MQ=255)
                                  cTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATg                                                         >  2:4333592/1‑94 (MQ=255)
                                  cTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATg                                                         >  2:2492861/1‑94 (MQ=255)
                                  cTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATg                                                         <  2:287905/94‑1 (MQ=255)
                                   ttACCCATTCTGAATGTGATTGGTGACGGTTCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGt                                                        <  1:2155919/94‑1 (MQ=255)
                                   ttAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGt                                                        <  2:9825271/94‑1 (MQ=255)
                                    tAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTc                                                       >  1:7011243/1‑94 (MQ=255)
                                     aaCCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCg                                                      >  1:10255451/1‑94 (MQ=255)
                                     aaCCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCg                                                      >  2:4515422/1‑94 (MQ=255)
                                     aaCCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCg                                                      <  1:8305622/94‑1 (MQ=255)
                                      aCCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGc                                                     >  2:4932361/1‑94 (MQ=255)
                                      aCCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGc                                                     >  1:4526890/1‑94 (MQ=255)
                                       ccATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCt                                                    >  1:6260349/1‑94 (MQ=255)
                                       ccATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCt                                                    <  2:5607708/94‑1 (MQ=255)
                                       ccATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCt                                                    <  2:7795117/94‑1 (MQ=255)
                                        cATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTg                                                   <  2:8919197/94‑1 (MQ=255)
                                         aTTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTgg                                                  <  1:5737939/94‑1 (MQ=255)
                                          ttCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGAG‑GCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGc                                                 <  2:5123149/94‑1 (MQ=255)
                                           tCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCg                                                <  2:3329061/94‑1 (MQ=255)
                                             tGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGAt                                              <  2:4575712/94‑1 (MQ=255)
                                              gAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGAtt                                             <  1:8071483/94‑1 (MQ=255)
                                              gAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGAtt                                             <  1:201102/94‑1 (MQ=255)
                                               aaTGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTg                                            <  1:8585549/94‑1 (MQ=255)
                                                aTGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGc                                           >  2:3200980/1‑94 (MQ=255)
                                                 tgtgATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGcc                                          >  2:5347585/1‑94 (MQ=255)
                                                 tgtgATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGcc                                          >  1:9995005/1‑94 (MQ=255)
                                                  gtgATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCa                                         <  2:7015216/94‑1 (MQ=255)
                                                   tgATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCAt                                        >  2:7071108/1‑94 (MQ=255)
                                                   tgATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCAt                                        <  2:10457904/94‑1 (MQ=255)
                                                    gATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATc                                       <  1:6757844/94‑1 (MQ=255)
                                                     gttGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATcg                                      <  1:7660917/93‑1 (MQ=255)
                                                     aTTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATcg                                      <  1:2718612/94‑1 (MQ=255)
                                                      ttgttgACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATcgc                                     <  1:8091069/94‑1 (MQ=255)
                                                         gTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCggg                                  >  1:6102570/1‑94 (MQ=255)
                                                         gTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCggg                                  <  1:7949476/94‑1 (MQ=255)
                                                            aCGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGgtgt                               >  2:7968170/1‑94 (MQ=255)
                                                             cGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAat                                                            >  1:1654801/1‑64 (MQ=255)
                                                             cGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGtgtg                              <  2:7292933/94‑1 (MQ=255)
                                                             cGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGtgtg                              >  2:178546/1‑94 (MQ=255)
                                                                tGCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTg                           >  1:10035044/1‑94 (MQ=255)
                                                                tGCTTTCTTCTGCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTg                           <  1:6512570/94‑1 (MQ=255)
                                                                 gCTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTgg                          <  2:3991973/94‑1 (MQ=255)
                                                                  cTTTCTTCTTCCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGt                         >  2:7699327/1‑94 (MQ=255)
                                                                      cttcttcCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACAt                     >  1:1378366/1‑94 (MQ=255)
                                                                          ttcCTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGa                 >  1:120146/1‑94 (MQ=255)
                                                                            ccTGCGGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGt               <  2:6865576/94‑1 (MQ=255)
                                                                            ccTGCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGt               <  2:5885418/94‑1 (MQ=255)
                                                                             cTGCCGCTGATGGTCG‑CGGCATCGGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTGCATCCGAGtt              <  1:7258802/94‑1 (MQ=255)
                                                                              cgCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGGCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGttt             <  1:5981897/93‑1 (MQ=255)
                                                                               gCCGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGtttt            <  2:6286158/94‑1 (MQ=255)
                                                                                ccGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTa           <  2:8244764/94‑1 (MQ=255)
                                                                                ccGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTa           >  1:879958/1‑94 (MQ=255)
                                                                                 cGCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTAt          >  2:5063305/1‑94 (MQ=255)
                                                                                  gCTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTAtt         <  2:1444174/94‑1 (MQ=255)
                                                                                  gCTGATGGTCG‑CGGCATCTGCCG‑TCATCAAATCTAAAACCAACATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGCTCAGCCGAGTTTGAtg         >  2:9396745/1‑93 (MQ=255)
                                                                                   cTGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTg        >  1:9178545/1‑94 (MQ=255)
                                                                                    tGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGa       <  1:5267601/94‑1 (MQ=255)
                                                                                    tGATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGa       <  1:3319818/94‑1 (MQ=255)
                                                                                    ggATGGTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGa       <  2:3568560/93‑1 (MQ=255)
                                                                                        ggTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAGCCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTg   <  1:9492883/94‑1 (MQ=255)
                                                                                        ggTCG‑CGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTg   >  1:3901808/1‑94 (MQ=255)
                                                                                               |                                                                                         
AGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCG‑C‑GCATCTGCCGCGCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGA  >  NC_000913/2837950‑2838132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 209 total aligned reads displayed.
GATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GC‑GCATCTGCCGCGCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCG  >  NC_000913/2837951‑2838137
                                                                                               |                                                                                             
GATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GC‑G                                                                                               <  SRR4302545.724149/94‑1 (MQ=60)
 ATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GC‑GG                                                                                              >  SRR4302545.3153797/1‑94 (MQ=60)
  TGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GC‑Ggc                                                                                             >  SRR4302545.2970131/1‑92 (MQ=60)
  TGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GC‑Ggc                                                                                             >  SRR4302545.4822818/1‑92 (MQ=60)
   GAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GC‑Ggca                                                                                            <  SRR4302545.4234726/94‑4 (MQ=60)
   GAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GC‑Ggca                                                                                            >  SRR4302545.1080668/1‑91 (MQ=60)
    AGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCAT                                                                                            >  SRR4302545.6453477/1‑94 (MQ=60)
    AGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCAT                                                                                            <  SRR4302545.621200/94‑1 (MQ=60)
    AGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCAT                                                                                            <  SRR4302545.7426544/94‑1 (MQ=60)
    AGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCAT                                                                                            >  SRR4302545.8776559/1‑94 (MQ=60)
     GCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATC                                                                                           <  SRR4302545.4763448/94‑1 (MQ=60)
      CGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCT                                                                                          <  SRR4302545.7109357/94‑1 (MQ=60)
      CGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCT                                                                                          <  SRR4302545.7743170/94‑1 (MQ=60)
       GGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTG                                                                                         >  SRR4302545.1773678/1‑94 (MQ=60)
       GGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTG                                                                                         <  SRR4302545.614413/94‑1 (MQ=60)
       GGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTG                                                                                         <  SRR4302545.1357363/94‑1 (MQ=60)
        GCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGC                                                                                        >  SRR4302545.8800447/1‑94 (MQ=60)
        GCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGC                                                                                        <  SRR4302545.7488831/94‑1 (MQ=60)
         CGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCC                                                                                       <  SRR4302545.7470003/94‑1 (MQ=60)
         CGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCC                                                                                       <  SRR4302545.4884997/94‑1 (MQ=60)
         CGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCC                                                                                       <  SRR4302545.152263/94‑1 (MQ=60)
         CGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCC                                                                                       <  SRR4302545.5753327/94‑1 (MQ=60)
         CGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCC                                                                                       <  SRR4302545.5146763/94‑1 (MQ=60)
         CGGGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCC                                                                                       <  SRR4302545.7383234/94‑1 (MQ=60)
          GTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCG                                                                                      <  SRR4302545.2717683/94‑1 (MQ=60)
            GCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGCC                                                                                    <  SRR4302545.316963/94‑1 (MQ=60)
             CTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGCca                                                                                   >  SRR4302545.187198/1‑92 (MQ=60)
              TGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCTCTGATGGTC‑GCGGCATCTGCCGC‑CAT                                                                                 <  SRR4302545.8115887/94‑1 (MQ=60)
              TGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CAT                                                                                 <  SRR4302545.7754393/94‑1 (MQ=60)
               GACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATC                                                                                <  SRR4302545.6400770/94‑1 (MQ=60)
               GACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATC                                                                                >  SRR4302545.9886494/1‑94 (MQ=60)
                ACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCA                                                                               <  SRR4302545.4118878/94‑1 (MQ=60)
                ACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCA                                                                               <  SRR4302545.9394551/94‑1 (MQ=60)
                ACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCA                                                                               <  SRR4302545.2397971/94‑1 (MQ=60)
                ACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCA                                                                               >  SRR4302545.4802833/1‑94 (MQ=60)
                 CAAAAGGATCTCCGACCTTAACCATTCTGAATGTGATTGGTTACTGTGGTTTCTTCTTCCTCCCGCTGATGGTCGGG‑GCATCTGCCGC‑CATCAA                                                                              >  SRR4302545.9037385/1‑94 (MQ=55)
                 CAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAA                                                                              >  SRR4302545.5262031/1‑94 (MQ=60)
                 CAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAA                                                                              <  SRR4302545.4696009/94‑1 (MQ=60)
                  AAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAA                                                                             >  SRR4302545.7237897/1‑94 (MQ=60)
                  AAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAA                                                                             >  SRR4302545.5409161/1‑94 (MQ=60)
                  AAAAGGATAGCCGACCTTAAACATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGACGC‑CATCAAA                                                                             >  SRR4302545.1462265/1‑94 (MQ=60)
                   AAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAAT                                                                            >  SRR4302545.92549/1‑94 (MQ=60)
                   AAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAAT                                                                            >  SRR4302545.2403835/1‑94 (MQ=60)
                   AAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAAT                                                                            <  SRR4302545.5105677/94‑1 (MQ=60)
                   AAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAAT                                                                            >  SRR4302545.10897023/1‑94 (MQ=60)
                    AAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATT                                                                           <  SRR4302545.10453918/94‑1 (MQ=60)
                     AGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTT                                                                          <  SRR4302545.289348/94‑1 (MQ=60)
                     AGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTT                                                                          >  SRR4302545.990164/1‑94 (MQ=60)
                      GGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTCCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTA                                                                         <  SRR4302545.857552/94‑1 (MQ=60)
                       GATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAA                                                                        <  SRR4302545.4926065/94‑1 (MQ=60)
                       GATCGCCAGCCTTAGCCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAA                                                                        <  SRR4302545.5201221/94‑1 (MQ=60)
                        ATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAA                                                                       <  SRR4302545.7116227/94‑1 (MQ=60)
                        ATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAA                                                                       >  SRR4302545.6343397/1‑94 (MQ=60)
                         TCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAA                                                                      <  SRR4302545.2185672/94‑1 (MQ=60)
                           GCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACC                                                                    >  SRR4302545.6663461/1‑94 (MQ=60)
                            CCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCA                                                                   >  SRR4302545.8767968/1‑94 (MQ=60)
                             CGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAA                                                                  >  SRR4302545.1842991/1‑94 (MQ=60)
                              GACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAAT                                                                 >  SRR4302545.775001/1‑94 (MQ=60)
                              GACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAAT                                                                 >  SRR4302545.7420829/1‑94 (MQ=60)
                              GACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAAT                                                                 >  SRR4302545.3876450/1‑94 (MQ=60)
                              GACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAAT                                                                 >  SRR4302545.5639959/1‑94 (MQ=60)
                              CACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAAT                                                                 <  SRR4302545.2123163/94‑1 (MQ=60)
                               ACCTTATCCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATA                                                                <  SRR4302545.316366/94‑1 (MQ=60)
                               ACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATA                                                                >  SRR4302545.2199294/1‑94 (MQ=60)
                               ACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATA                                                                >  SRR4302545.5072276/1‑94 (MQ=60)
                               ACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATA                                                                <  SRR4302545.7379672/94‑1 (MQ=60)
                                CCTTACCCACTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATAT                                                               <  SRR4302545.4476906/94‑1 (MQ=60)
                                CCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATAT                                                               <  SRR4302545.1888066/94‑1 (MQ=60)
                                CCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATAT                                                               <  SRR4302545.8169336/94‑1 (MQ=60)
                                CCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATAT                                                               <  SRR4302545.961258/94‑1 (MQ=60)
                                CCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATAT                                                               <  SRR4302545.4476907/94‑1 (MQ=60)
                                CCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATAC                                                               <  SRR4302545.2427654/94‑1 (MQ=60)
                                 CTTAACCATTCTGAAGGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATG                                                              <  SRR4302545.9458501/94‑1 (MQ=60)
                                  TTACCCATTCTGAATGTGATTGGTGACGGTTCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGT                                                             <  SRR4302545.2208647/94‑1 (MQ=60)
                                  TTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGTTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGT                                                             <  SRR4302545.1721360/94‑1 (MQ=60)
                                  TTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGT                                                             >  SRR4302545.8872991/1‑94 (MQ=60)
                                  TTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGT                                                             <  SRR4302545.10160128/94‑1 (MQ=60)
                                   TAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTC                                                            >  SRR4302545.7204705/1‑94 (MQ=60)
                                    AACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCG                                                           >  SRR4302545.2922802/1‑94 (MQ=60)
                                    AACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCG                                                           >  SRR4302545.5273179/1‑94 (MQ=60)
                                    AACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCG                                                           <  SRR4302545.7475607/94‑1 (MQ=60)
                                    AACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCG                                                           >  SRR4302545.10558119/1‑94 (MQ=60)
                                    AACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCG                                                           <  SRR4302545.8546254/94‑1 (MQ=60)
                                    AACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCG                                                           <  SRR4302545.6453349/94‑1 (MQ=60)
                                     ACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGC                                                          >  SRR4302545.4642871/1‑94 (MQ=60)
                                     ACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGC                                                          >  SRR4302545.4642872/1‑94 (MQ=60)
                                      CCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCT                                                         >  SRR4302545.6431007/1‑94 (MQ=60)
                                      CCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCT                                                         <  SRR4302545.3985138/94‑1 (MQ=60)
                                       CATTCTGATTGTGCTTGGTGACGGTGCTTTCTTCTACCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTG                                                        <  SRR4302545.10069261/94‑1 (MQ=60)
                                       CATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTG                                                        <  SRR4302545.984267/94‑1 (MQ=60)
                                        ATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGG                                                       <  SRR4302545.5891953/94‑1 (MQ=60)
                                         TTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGC                                                      <  SRR4302545.366243/94‑1 (MQ=60)
                                         TTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGC                                                      >  SRR4302545.1170323/1‑94 (MQ=60)
                                          TCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCG                                                     >  SRR4302545.5387823/1‑94 (MQ=60)
                                            TGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTTTCGAT                                                   >  SRR4302545.7987511/1‑94 (MQ=60)
                                             GAATGTGATTGGTGCCGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATT                                                  <  SRR4302545.6836977/94‑1 (MQ=60)
                                             GAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATT                                                  <  SRR4302545.1472269/94‑1 (MQ=60)
                                             GAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATT                                                  <  SRR4302545.8302768/94‑1 (MQ=60)
                                             GAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATT                                                  <  SRR4302545.205294/94‑1 (MQ=60)
                                              AATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTG                                                 <  SRR4302545.8833905/94‑1 (MQ=60)
                                              AATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTG                                                 <  SRR4302545.502943/94‑1 (MQ=60)
                                              AATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTG                                                 >  SRR4302545.5228221/1‑94 (MQ=60)
                                               ATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGC                                                >  SRR4302545.305723/1‑94 (MQ=60)
                                                TGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCC                                               >  SRR4302545.10291367/1‑94 (MQ=60)
                                                TGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCC                                               <  SRR4302545.7764269/94‑1 (MQ=60)
                                                 GTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCA                                              >  SRR4302545.2956977/1‑94 (MQ=60)
                                                 GTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCA                                              >  SRR4302545.2396855/1‑94 (MQ=60)
                                                 GTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCA                                              >  SRR4302545.8578150/1‑94 (MQ=60)
                                                  TGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCAT                                             <  SRR4302545.9775219/94‑1 (MQ=60)
                                                  TGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCAT                                             >  SRR4302545.318812/1‑94 (MQ=60)
                                                  TGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCAT                                             >  SRR4302545.7626598/1‑94 (MQ=60)
                                                  TGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCAT                                             <  SRR4302545.10559156/94‑1 (MQ=60)
                                                   tattatgGACGGTACTTTCTTCTTCCTGCCGCTTATGGTC‑GCGGCATCTGCCGC‑AATCAAATTTAAATCCAATATGTCGCTGGCGATTGCCATC                                            <  SRR4302545.1817925/87‑1 (MQ=60)
                                                   GATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATC                                            <  SRR4302545.555159/94‑1 (MQ=60)
                                                   GATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATC                                            <  SRR4302545.6943139/94‑1 (MQ=60)
                                                    GTTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCG                                           <  SRR4302545.7877728/94‑1 (MQ=60)
                                                    ATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCG                                           >  SRR4302545.4568232/1‑94 (MQ=60)
                                                    ATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCG                                           <  SRR4302545.2784169/94‑1 (MQ=60)
                                                    ATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCG                                           <  SRR4302545.1658831/94‑1 (MQ=60)
                                                     TTGTTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGC                                          <  SRR4302545.8324279/94‑1 (MQ=60)
                                                      TGGTGTCGGTGCTGTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCG                                         <  SRR4302545.8317829/94‑1 (MQ=60)
                                                      TGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCG                                         <  SRR4302545.8317828/94‑1 (MQ=60)
                                                        GTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGG                                       <  SRR4302545.8175366/94‑1 (MQ=60)
                                                        GTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGG                                       >  SRR4302545.6268397/1‑94 (MQ=60)
                                                         TGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGT                                      <  SRR4302545.1092457/94‑1 (MQ=60)
                                                         TGACGGTGCTTTCTTCTCCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGT                                      <  SRR4302545.3977150/94‑1 (MQ=60)
                                                          GACGTTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTG                                     <  SRR4302545.2605386/94‑1 (MQ=60)
                                                          GACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTG                                     >  SRR4302545.83750/1‑94 (MQ=60)
                                                           ACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGT                                    <  SRR4302545.10644405/94‑1 (MQ=60)
                                                            CGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATAgatcagagatcggaagagcgtcgtgtagg                                   >  SRR4302545.1693974/1‑65 (MQ=60)
                                                            CGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTG                                   <  SRR4302545.4370615/94‑1 (MQ=60)
                                                             GGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGC                                  <  SRR4302545.6301753/94‑1 (MQ=60)
                                                             GGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGC                                  >  SRR4302545.4801681/1‑94 (MQ=60)
                                                             GGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGC                                  >  SRR4302545.2626746/1‑94 (MQ=60)
                                                             GGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGGGTGC                                  >  SRR4302545.4054473/1‑94 (MQ=60)
                                                               TGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTG                                <  SRR4302545.8432690/94‑1 (MQ=60)
                                                               TGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTG                                <  SRR4302545.568366/94‑1 (MQ=60)
                                                               TGCTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTG                                >  SRR4302545.10332314/1‑94 (MQ=60)
                                                               TGCTTTCTTCTGCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTG                                <  SRR4302545.6690696/94‑1 (MQ=60)
                                                                 CTTTCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGT                              <  SRR4302545.356627/94‑1 (MQ=60)
                                                                 CTTTCTCCTTCCCCCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGT                              <  SRR4302545.2720910/94‑1 (MQ=60)
                                                                    TCTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACA                           <  SRR4302545.603611/94‑1 (MQ=60)
                                                                     CTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACAT                          >  SRR4302545.1409261/1‑94 (MQ=60)
                                                                     CTTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACAT                          >  SRR4302545.1409260/1‑94 (MQ=60)
                                                                      TTCTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATC                         <  SRR4302545.6199720/94‑1 (MQ=60)
                                                                        CTTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCG                       >  SRR4302545.2513164/1‑94 (MQ=60)
                                                                         TTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGA                      >  SRR4302545.4628399/1‑94 (MQ=60)
                                                                         TTCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGA                      >  SRR4302545.122870/1‑94 (MQ=60)
                                                                          TCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAG                     <  SRR4302545.8890669/94‑1 (MQ=60)
                                                                          TCCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAG                     <  SRR4302545.5634653/94‑1 (MQ=60)
                                                                           CCTGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGT                    <  SRR4302545.3055459/94‑1 (MQ=60)
                                                                            CTGCCGCTGATGGTC‑GCGGCATCGGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTGCATCCGAGTT                   <  SRR4302545.7462192/94‑1 (MQ=60)
                                                                            CTGCCGCTGATGGTC‑GCGGCATCGGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTGCATCCGAGTT                   <  SRR4302545.7462193/94‑1 (MQ=60)
                                                                            CTCCCGCTGATGGTC‑GCGGCATGTGCCAC‑CATCAAATTTAAAGCCAATATGTGGCTGGCGATTGCCATCGCAGGTGTGCTGGTACATCCGAGTT                   <  SRR4302545.6139315/94‑1 (MQ=57)
                                                                             CGCCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGGCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTT                  <  SRR4302545.6143282/94‑1 (MQ=60)
                                                                               CCGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTA                >  SRR4302545.899709/1‑94 (MQ=60)
                                                                                CGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTAT               >  SRR4302545.7331587/1‑94 (MQ=60)
                                                                                CGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTAT               >  SRR4302545.8258729/1‑94 (MQ=60)
                                                                                CGCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTAT               >  SRR4302545.4126433/1‑94 (MQ=60)
                                                                                 GCTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATT              <  SRR4302545.1635470/94‑1 (MQ=60)
                                                                                  CTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTG             <  SRR4302545.1043902/94‑1 (MQ=60)
                                                                                  CTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTG             >  SRR4302545.8636207/1‑94 (MQ=60)
                                                                                  CTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTG             >  SRR4302545.1799138/1‑94 (MQ=60)
                                                                                  CTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTG             >  SRR4302545.9447748/1‑94 (MQ=60)
                                                                                  CTGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTG             >  SRR4302545.10816333/1‑94 (MQ=60)
                                                                                   TGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGA            <  SRR4302545.5403806/94‑1 (MQ=60)
                                                                                   TGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGA            <  SRR4302545.3400961/94‑1 (MQ=60)
                                                                                   TGATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGCGTGTGCTGGTACATCCGCGGTTTATTGA            >  SRR4302545.6648708/1‑94 (MQ=60)
                                                                                    GCTGGTC‑GCGGCATCTGGCGC‑CATCAAATTTAAAACCAATATGTCGCTTGCGATTGCCATCGCGGGTGTGCTGGTACATTCGAGTTTTATTGAA           >  SRR4302545.5947804/1‑94 (MQ=60)
                                                                                    GATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAA           >  SRR4302545.2433465/1‑94 (MQ=60)
                                                                                     ATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTGCATCCGAGTTTTATTGAAC          <  SRR4302545.8255814/94‑1 (MQ=60)
                                                                                     ATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTGCATCCGAGTTTTATTGAAC          <  SRR4302545.8255815/94‑1 (MQ=60)
                                                                                     ATGGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAAC          <  SRR4302545.4313301/94‑1 (MQ=60)
                                                                                       GGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAGCCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTG        <  SRR4302545.9772296/94‑1 (MQ=60)
                                                                                       GGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTG        <  SRR4302545.9458904/94‑1 (MQ=60)
                                                                                       GGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTG        >  SRR4302545.3426752/1‑94 (MQ=60)
                                                                                       GGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTG        >  SRR4302545.3999187/1‑94 (MQ=60)
                                                                                       GGTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTG        <  SRR4302545.827537/94‑1 (MQ=60)
                                                                                        GTC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTGCATCCGAGTTTTATTGAACTGA       <  SRR4302545.1272306/94‑1 (MQ=60)
                                                                                         TC‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGAT      <  SRR4302545.3015789/94‑1 (MQ=60)
                                                                                          gcccgGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGAT      <  SRR4302545.7718365/89‑1 (MQ=60)
                                                                                          C‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGTTGGCGTTTGCCATCGCGGGTGTGCTGGTACGTCCTTGTTTTTTTGAACTGATG     <  SRR4302545.9044287/94‑1 (MQ=60)
                                                                                          C‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATG     <  SRR4302545.4413577/94‑1 (MQ=60)
                                                                                          C‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATG     >  SRR4302545.3682074/1‑94 (MQ=60)
                                                                                          C‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATG     <  SRR4302545.3473534/94‑1 (MQ=60)
                                                                                          C‑GCGGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATG     <  SRR4302545.5982462/94‑1 (MQ=60)
                                                                                          C‑GCGGCATCTGCCGC‑CATCAAATCTAAAACCAATATGTCGCTGGCGATGGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATG     <  SRR4302545.1232420/94‑1 (MQ=60)
                                                                                            gcgGCATCTGCCGC‑CATCAAATTTAAACCCAATATGTCGCGGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGAGGG    <  SRR4302545.4869380/91‑1 (MQ=60)
                                                                                            gcgGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGG    >  SRR4302545.6059781/4‑94 (MQ=60)
                                                                                            gcgGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGG    <  SRR4302545.4869381/91‑1 (MQ=60)
                                                                                            gcgGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGG    <  SRR4302545.9320699/91‑1 (MQ=60)
                                                                                            gcgGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGG    >  SRR4302545.5159175/4‑94 (MQ=60)
                                                                                             cgGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGC   <  SRR4302545.2305934/92‑1 (MQ=60)
                                                                                             cgGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGC   <  SRR4302545.5415990/92‑1 (MQ=60)
                                                                                             cgGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGC   <  SRR4302545.9780825/92‑1 (MQ=60)
                                                                                             cgGCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGC   >  SRR4302545.4606256/3‑94 (MQ=60)
                                                                                             G‑GCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCG  >  SRR4302545.394612/1‑94 (MQ=60)
                                                                                             G‑GCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCG  >  SRR4302545.9569961/1‑94 (MQ=60)
                                                                                             G‑GCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCG  >  SRR4302545.9569963/1‑94 (MQ=60)
                                                                                             G‑GCATCTGCCGC‑CATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCG  <  SRR4302545.8140106/94‑1 (MQ=60)
                                                                                               |                                                                                             
GATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTC‑GC‑GCATCTGCCGCGCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCG  >  NC_000913/2837951‑2838137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑