Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,078,245 C→G G470G (GGG→GGC)  tktA ← transketolase 1 isozyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,078,2450CG99.7% 1222.2 / NA 370G470G (GGG→GGC) tktAtransketolase 1 isozyme
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (179/190);  minor base T (0/1);  total (179/191)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 404 total aligned reads displayed.

CCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCG  >  NC_000913/3078154‑3078335
                                                                                           |                                                                                          
ccGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGgcc                                                                                          >  1:4762329/1‑94 (MQ=255)
ccGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGgcc                                                                                          <  1:8053699/94‑1 (MQ=255)
ccGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGgcc                                                                                          <  2:5154338/94‑1 (MQ=255)
ccGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGgcc                                                                                          <  1:22188/94‑1 (MQ=255)
 cgcgGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCg                                                                                         >  2:5421174/1‑94 (MQ=255)
  gcgGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGt                                                                                        >  2:6420388/1‑94 (MQ=255)
   cgGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTc                                                                                       <  1:4668340/94‑1 (MQ=255)
    ggATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCt                                                                                      >  1:7212772/1‑94 (MQ=255)
    ggATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCt                                                                                      <  1:2692359/94‑1 (MQ=255)
      aTTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCttg                                                                                    >  1:5217352/1‑93 (MQ=255)
      aTTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTc                                                                                    >  2:453038/1‑94 (MQ=255)
        tCAACCTAGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGc                                                                                  >  1:10281670/1‑94 (MQ=255)
           aCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAATCGGGCCGTCTTCGCCCa                                                                               <  1:2555281/94‑1 (MQ=255)
            ccTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAg                                                                              <  2:2990747/94‑1 (MQ=255)
            ccTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGACTTCGCGCAg                                                                              >  1:1407400/1‑94 (MQ=255)
            ccTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCAGTCTTCGccccg                                                                              >  1:4523179/1‑92 (MQ=255)
             cTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGa                                                                             >  2:1008404/1‑94 (MQ=255)
               ggTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGAcc                                                                           >  2:7335247/1‑94 (MQ=255)
                  cacaCGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAt                                                                        <  2:4061727/94‑1 (MQ=255)
                  cacaCGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAt                                                                        <  2:1899680/94‑1 (MQ=255)
                  cacaCGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAt                                                                        <  2:821803/94‑1 (MQ=255)
                   acacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATg                                                                       >  2:8742988/1‑94 (MQ=255)
                   acacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATg                                                                       >  1:6962012/1‑94 (MQ=255)
                   acacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATg                                                                       >  2:3451833/1‑94 (MQ=255)
                      cGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAg                                                                    >  2:4831147/1‑94 (MQ=255)
                      cGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAg                                                                    <  1:7684892/94‑1 (MQ=255)
                       ggACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGt                                                                   >  2:2038581/1‑94 (MQ=255)
                        gACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTc                                                                  <  2:8509200/94‑1 (MQ=255)
                        gACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTc                                                                  >  2:8611432/1‑94 (MQ=255)
                        gACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTc                                                                  >  1:3561872/1‑94 (MQ=255)
                         aCGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCg                                                                 <  2:10458092/94‑1 (MQ=255)
                         aCGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCg                                                                 <  2:9274639/94‑1 (MQ=255)
                         aCGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCg                                                                 <  2:2467619/94‑1 (MQ=255)
                          cGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGGCCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGt                                                                <  1:5468580/94‑1 (MQ=255)
                          cGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGTCGTCTTCGCCCAGACCGATGGAGTCGt                                                                >  1:8286863/1‑94 (MQ=255)
                          cGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGTCCGATGGAGTCGt                                                                >  2:3905738/1‑94 (MQ=255)
                          cGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCga                                                                >  2:44417/1‑93 (MQ=255)
                          cGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGt                                                                >  2:1095198/1‑94 (MQ=255)
                          cGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGt                                                                >  2:6000802/1‑94 (MQ=255)
                           gCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTg                                                               >  1:8633312/1‑94 (MQ=255)
                            ccATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTgg                                                              >  2:606202/1‑94 (MQ=255)
                             cATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTggg                                                             <  2:18082/94‑1 (MQ=255)
                              aTGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTgggg                                                            >  1:2796533/1‑93 (MQ=255)
                              aTGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgt                                                            >  1:5619415/1‑94 (MQ=255)
                              aTGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgt                                                            >  2:4671156/1‑94 (MQ=255)
                                 tAGACATGTTCGGGGGTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGACGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTa                                                         >  2:6681253/1‑94 (MQ=255)
                                  aGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTCGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTaa                                                        >  1:6308369/1‑94 (MQ=255)
                                    aCATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGGCGTGGGTGTAAAc                                                      >  2:8317447/1‑94 (MQ=255)
                                     cATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAcc                                                     <  1:10009192/94‑1 (MQ=255)
                                     cATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGGCTTCGCCCAGACCGATGGAGTCGTGGGTGTaaaac                                                     >  1:7949772/1‑92 (MQ=255)
                                      aTGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCa                                                    >  2:2719289/1‑94 (MQ=255)
                                      aTGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCa                                                    <  1:1531890/94‑1 (MQ=255)
                                      aTGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCa                                                    <  1:1693637/94‑1 (MQ=255)
                                       tGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCAt                                                   >  1:6840655/1‑94 (MQ=255)
                                        gTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATc                                                  <  2:3199771/94‑1 (MQ=255)
                                        gTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATc                                                  >  2:9636531/1‑94 (MQ=255)
                                           cGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCAcc                                               <  1:1269755/94‑1 (MQ=255)
                                            ggggTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCt                                              <  2:3362053/94‑1 (MQ=255)
                                             gggTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCta                                             >  1:9103271/1‑93 (MQ=255)
                                              ggTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGa                                            >  1:6564980/1‑94 (MQ=255)
                                              ggTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGa                                            <  1:10518426/94‑1 (MQ=255)
                                               gTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGAc                                           >  1:8416508/1‑94 (MQ=255)
                                                    gcgcAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGt                                      <  1:245947/94‑1 (MQ=255)
                                                    gcgcAGAGAAGCGACCTGCTCAACCGGCTGGGGAGTCGGGCCGTCTTCGCCCAGACCGCTGGAGTCGTGGGTGTAAACCATCACCTGACGCTGt                                      >  2:10175338/1‑94 (MQ=255)
                                                     ggcAGAGAAGCGACCTGCTCAGCCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGtt                                     <  2:5859680/93‑1 (MQ=255)
                                                       cAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtc                                   >  2:168533/1‑94 (MQ=255)
                                                       cAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtc                                   >  2:9037231/1‑94 (MQ=255)
                                                        agagAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtca                                  <  2:9852127/94‑1 (MQ=255)
                                                        agagAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtca                                  >  1:1114667/1‑94 (MQ=255)
                                                            aaGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAg                              >  2:7435153/1‑94 (MQ=255)
                                                            aaGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAg                              <  1:1759830/94‑1 (MQ=255)
                                                             aGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgc                             <  1:3347882/94‑1 (MQ=255)
                                                             aGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgc                             >  1:3822265/1‑94 (MQ=255)
                                                             aGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgc                             <  2:5652356/94‑1 (MQ=255)
                                                             aGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgc                             >  1:3898275/1‑94 (MQ=255)
                                                              gCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgcg                            <  2:7510209/94‑1 (MQ=255)
                                                              gCGACCTGCTAAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgcg                            <  1:400163/94‑1 (MQ=255)
                                                               cGACCTGCTCAACCGGCTGGTGAGTCGGGCCGGCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgcgc                           >  1:9599777/1‑94 (MQ=255)
                                                                gACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCa                          <  2:8939693/94‑1 (MQ=255)
                                                                gACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCa                          <  2:6292669/94‑1 (MQ=255)
                                                                 aCCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAg                         <  1:6291207/94‑1 (MQ=255)
                                                                   cTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGTGTCGTGGGTGTAAACGATCCCCTGACGCTGTTTCATCAGTGCAGcc                       >  1:3136473/1‑94 (MQ=255)
                                                                   cTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGcc                       >  1:4577338/1‑94 (MQ=255)
                                                                      cTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAta                    <  2:7165981/94‑1 (MQ=255)
                                                                       tCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtac                   <  2:2337234/94‑1 (MQ=255)
                                                                        cAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacg                  >  2:210031/1‑94 (MQ=255)
                                                                         aaCCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATAACCTGACGCTGTTTCATCAGCGCAGCCAtacgt                 >  1:5343381/1‑94 (MQ=255)
                                                                          aCCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCCTCAGCGCAGCCAtacgta                >  1:587919/1‑94 (MQ=255)
                                                                           ccGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgtac               <  2:8013517/94‑1 (MQ=255)
                                                                           ccGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgtac               <  1:8955369/94‑1 (MQ=255)
                                                                           ccGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgtac               <  1:9467108/94‑1 (MQ=255)
                                                                            cGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCAtacgtacg              >  2:1268995/1‑94 (MQ=255)
                                                                             cgCTGGTGGGGCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACgg             <  2:1426710/93‑1 (MQ=255)
                                                                               cTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGCCGCTGTTTCATCAGCGCAGCCATACGTACGGCg           <  1:9897730/94‑1 (MQ=255)
                                                                                  gTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTa        <  1:8370083/94‑1 (MQ=255)
                                                                                      ggcGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTg    <  1:193797/92‑1 (MQ=255)
                                                                                      gTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTg    <  1:6708019/94‑1 (MQ=255)
                                                                                       tCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGc   <  1:7396409/94‑1 (MQ=255)
                                                                                        cGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCg  >  1:5062924/1‑94 (MQ=255)
                                                                                        cGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCg  >  2:6862831/1‑94 (MQ=255)
                                                                                           |                                                                                          
CCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGCCATACGTACGGCGTTACGTGCG  >  NC_000913/3078154‑3078335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 265 total aligned reads displayed.
AGCACATGGATTTTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGTGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGA  >  NC_000913/3084008‑3084194
                                                                                             |                                                                                             
AGCACATGGATTTTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGC                                                                                               <  SRR4302545.3585268/94‑1 (MQ=60)
AGCACATGGATTTTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGC                                                                                               >  SRR4302545.5979774/1‑94 (MQ=60)
 GCACATGGATTTTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGAAAGCG                                                                                              <  SRR4302545.9676165/94‑1 (MQ=60)
  CACATGGATTTTCCAGCAGTGAGTGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGCACGTAAGCGT                                                                                             >  SRR4302545.4660339/1‑94 (MQ=60)
  CACATGGATTTTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGT                                                                                             >  SRR4302545.7874497/1‑94 (MQ=60)
   CCATGGATTTTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTG                                                                                            <  SRR4302545.10039976/94‑1 (MQ=60)
   ACATGGATTTTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTG                                                                                            <  SRR4302545.8442713/94‑1 (MQ=60)
   ACATGGATTTTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTG                                                                                            <  SRR4302545.1103978/94‑1 (MQ=60)
    CATGGATTTTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGA                                                                                           >  SRR4302545.2486230/1‑94 (MQ=60)
    CATGGATTTTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGA                                                                                           >  SRR4302545.7284235/1‑94 (MQ=60)
    CATGGATTTTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGA                                                                                           <  SRR4302545.9274982/94‑1 (MQ=60)
     ATGGATTTTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAA                                                                                          <  SRR4302545.8139591/94‑1 (MQ=60)
     ATGGATTTTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAA                                                                                          >  SRR4302545.57436/1‑94 (MQ=60)
       GGATTTTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAG                                                                                        <  SRR4302545.2806420/94‑1 (MQ=60)
        GATTTTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGT                                                                                       >  SRR4302545.1552108/1‑94 (MQ=60)
         ATTTTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTT                                                                                      <  SRR4302545.7036508/94‑1 (MQ=60)
         ATTTTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTT                                                                                      >  SRR4302545.4858692/1‑94 (MQ=60)
          TTTTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTC                                                                                     <  SRR4302545.495773/94‑1 (MQ=60)
          TTTTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTC                                                                                     <  SRR4302545.9107353/94‑1 (MQ=60)
          TTTTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTC                                                                                     <  SRR4302545.3898543/94‑1 (MQ=60)
          TTTTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTC                                                                                     <  SRR4302545.930111/94‑1 (MQ=60)
          TTTTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTC                                                                                     <  SRR4302545.542529/94‑1 (MQ=60)
          TTTTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTC                                                                                     <  SRR4302545.4295397/94‑1 (MQ=60)
           TTTCCAGTAGTGACTGCTGACGCTCCAACTGCGCAGCCGGTTGCTTTTTCGTAACATTATTAAGCACATAGCCGAACGTAAGCGTGAAAGTTCG                                                                                    <  SRR4302545.1415155/94‑1 (MQ=60)
           TTTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCG                                                                                    <  SRR4302545.5085761/94‑1 (MQ=60)
            TTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTTATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGG                                                                                   >  SRR4302545.7230677/1‑94 (MQ=60)
            TTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGG                                                                                   >  SRR4302545.9458820/1‑94 (MQ=60)
            TTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGG                                                                                   <  SRR4302545.1604867/94‑1 (MQ=60)
            TTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGG                                                                                   >  SRR4302545.7230676/1‑94 (MQ=60)
             TCCAGAAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGC                                                                                  >  SRR4302545.10776729/1‑94 (MQ=60)
              CCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCG                                                                                 <  SRR4302545.3533090/94‑1 (MQ=60)
              CCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCG                                                                                 <  SRR4302545.7651780/94‑1 (MQ=60)
              CCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAACACATAACCGAACGTAAGCGTGAAAGTTCGGCG                                                                                 >  SRR4302545.2406878/1‑94 (MQ=60)
              CCAGCAGTGAATGCTGACGCTCCAACGGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGGTCGGCG                                                                                 <  SRR4302545.7341252/94‑1 (MQ=60)
               CAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGA                                                                                >  SRR4302545.5983524/1‑94 (MQ=60)
               CAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGA                                                                                >  SRR4302545.8550086/1‑94 (MQ=60)
                AGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAA                                                                               >  SRR4302545.6033852/1‑94 (MQ=60)
                AGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAA                                                                               >  SRR4302545.286222/1‑94 (MQ=60)
                AGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTAGTAAGCACAGAGCCGAACGTAAGCGTGAAAGTTCGGCGAA                                                                               <  SRR4302545.8862865/94‑1 (MQ=60)
                AGCAGTGAATGCTGACCCTCCAACTGCGGAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGACCGTAAGCGTGAAAGTTCGGCGAA                                                                               <  SRR4302545.8862864/94‑1 (MQ=60)
                 GCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAA                                                                              >  SRR4302545.2363711/1‑94 (MQ=60)
                  CAGTGACTGCTGACGCTCCAACTGCGCAACCGGCCACTTTTTCATAACATTATTAAGCACATAATCGAACGTAAGCGTGAAAGTTCGGCGACAC                                                                             >  SRR4302545.10212116/1‑94 (MQ=60)
                  CAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAAC                                                                             <  SRR4302545.9753065/94‑1 (MQ=60)
                  CAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAAC                                                                             <  SRR4302545.4677758/94‑1 (MQ=60)
                   AGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACTTAACCGAACGTAAGCGTGAAAGTTCGGCGAAACC                                                                            >  SRR4302545.3310124/1‑94 (MQ=60)
                   AGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTCAGCGTGAAAGTTCGGCGAAACC                                                                            <  SRR4302545.6946578/94‑1 (MQ=60)
                   AGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACC                                                                            >  SRR4302545.6693372/1‑94 (MQ=60)
                   AGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACC                                                                            >  SRR4302545.2330249/1‑94 (MQ=60)
                   AGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACC                                                                            <  SRR4302545.4677099/94‑1 (MQ=60)
                    GTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGACACCA                                                                           >  SRR4302545.9806994/1‑94 (MQ=60)
                    GTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCA                                                                           >  SRR4302545.9806993/1‑94 (MQ=60)
                    GTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCA                                                                           >  SRR4302545.7979105/1‑94 (MQ=60)
                    GTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCA                                                                           <  SRR4302545.8637508/94‑1 (MQ=60)
                    GTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCA                                                                           <  SRR4302545.5812240/94‑1 (MQ=60)
                     TGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCAC                                                                          <  SRR4302545.5533715/94‑1 (MQ=60)
                     TGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCAC                                                                          <  SRR4302545.8466871/94‑1 (MQ=60)
                     TGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCAC                                                                          >  SRR4302545.3070460/1‑94 (MQ=60)
                      GAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACG                                                                         <  SRR4302545.9388536/94‑1 (MQ=60)
                      GAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACG                                                                         >  SRR4302545.6148493/1‑94 (MQ=60)
                       AATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGA                                                                        <  SRR4302545.7422192/94‑1 (MQ=60)
                        ATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAG                                                                       <  SRR4302545.5046030/94‑1 (MQ=60)
                        ATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAG                                                                       <  SRR4302545.2834053/94‑1 (MQ=60)
                            TGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAA                                                                   >  SRR4302545.4822838/1‑94 (MQ=60)
                             GACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAAC                                                                  <  SRR4302545.4025845/94‑1 (MQ=60)
                             GACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAAC                                                                  >  SRR4302545.6126991/1‑94 (MQ=60)
                              ACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACT                                                                 >  SRR4302545.759977/1‑94 (MQ=60)
                               CGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTT                                                                >  SRR4302545.2268887/1‑94 (MQ=60)
                               CGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTC                                                                >  SRR4302545.4774603/1‑94 (MQ=60)
                                 CTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGCACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTATT                                                              >  SRR4302545.8191723/1‑94 (MQ=60)
                                 CTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTT                                                              <  SRR4302545.5625633/94‑1 (MQ=60)
                                 CTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTT                                                              <  SRR4302545.5625632/94‑1 (MQ=60)
                                  TCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTG                                                             >  SRR4302545.1846413/1‑94 (MQ=60)
                                  TCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTG                                                             <  SRR4302545.1201810/94‑1 (MQ=60)
                                  TCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTG                                                             <  SRR4302545.2318101/94‑1 (MQ=60)
                                   CCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGT                                                            <  SRR4302545.1933514/94‑1 (MQ=60)
                                   CCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGT                                                            <  SRR4302545.6115043/94‑1 (MQ=60)
                                   CCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGT                                                            <  SRR4302545.6500258/94‑1 (MQ=60)
                                    CAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTT                                                           <  SRR4302545.7999037/94‑1 (MQ=60)
                                    CAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTT                                                           >  SRR4302545.5282907/1‑94 (MQ=60)
                                    CAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTT                                                           >  SRR4302545.7642472/1‑94 (MQ=60)
                                    CAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTT                                                           <  SRR4302545.896920/94‑1 (MQ=60)
                                    CAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTT                                                           >  SRR4302545.450451/1‑94 (MQ=60)
                                    CAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTT                                                           <  SRR4302545.962516/94‑1 (MQ=60)
                                     AACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTT                                                          >  SRR4302545.2224392/1‑94 (MQ=60)
                                      ACTGCGCAACTGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGTGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTT                                                         <  SRR4302545.7433234/94‑1 (MQ=60)
                                      ACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTT                                                         <  SRR4302545.3679780/94‑1 (MQ=60)
                                      ACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTT                                                         <  SRR4302545.3827654/94‑1 (MQ=60)
                                       CTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTT                                                        <  SRR4302545.6771927/94‑1 (MQ=60)
                                        TGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTA                                                       <  SRR4302545.7277182/94‑1 (MQ=60)
                                        TGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTA                                                       >  SRR4302545.6884018/1‑94 (MQ=60)
                                        TGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTA                                                       <  SRR4302545.2995897/94‑1 (MQ=60)
                                         GCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTAC                                                      >  SRR4302545.10839698/1‑94 (MQ=60)
                                         GCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTAC                                                      <  SRR4302545.4723732/94‑1 (MQ=60)
                                          CGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACA                                                     <  SRR4302545.9308945/94‑1 (MQ=60)
                                          CGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACA                                                     <  SRR4302545.207467/94‑1 (MQ=60)
                                          CGCAACCGGTATCCATTTCATAACCTTATTAAGCACATAACCGAAAGTAAGCGTGAAAGTTCGGCCAAACCACGAGAAAACTCTTGTTTCTACA                                                     >  SRR4302545.4460491/1‑94 (MQ=60)
                                            CAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAG                                                   <  SRR4302545.10389181/94‑1 (MQ=60)
                                            CAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAG                                                   <  SRR4302545.4399614/94‑1 (MQ=60)
                                            CAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAG                                                   <  SRR4302545.3475752/94‑1 (MQ=60)
                                             AACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGA                                                  <  SRR4302545.8900531/94‑1 (MQ=60)
                                             AACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGA                                                  <  SRR4302545.8521279/94‑1 (MQ=60)
                                             AACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGA                                                  >  SRR4302545.3588938/1‑94 (MQ=60)
                                              ACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAG                                                 <  SRR4302545.5769492/94‑1 (MQ=60)
                                               CCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGC                                                >  SRR4302545.673316/1‑94 (MQ=60)
                                               CCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGC                                                <  SRR4302545.2106355/94‑1 (MQ=60)
                                               CCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGC                                                >  SRR4302545.8069675/1‑94 (MQ=60)
                                               CCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGC                                                <  SRR4302545.1367161/94‑1 (MQ=60)
                                                CGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCG                                               >  SRR4302545.7923394/1‑94 (MQ=60)
                                                CGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCG                                               <  SRR4302545.6910792/94‑1 (MQ=60)
                                                 GGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGC                                              >  SRR4302545.4018363/1‑94 (MQ=60)
                                                 GGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGC                                              >  SRR4302545.3771519/1‑94 (MQ=60)
                                                  GTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCC                                             >  SRR4302545.1263857/1‑94 (MQ=60)
                                                   TTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCC                                            <  SRR4302545.4063973/94‑1 (MQ=60)
                                                   TTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCC                                            >  SRR4302545.3677727/1‑94 (MQ=60)
                                                   TTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCC                                            >  SRR4302545.9494943/1‑94 (MQ=60)
                                                   TTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGACCGCCC                                            >  SRR4302545.4977822/1‑94 (MQ=60)
                                                    TTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCT                                           >  SRR4302545.1200484/1‑94 (MQ=60)
                                                      CTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGACACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTG                                         >  SRR4302545.7841626/1‑94 (MQ=60)
                                                      CTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTG                                         >  SRR4302545.9836974/1‑94 (MQ=60)
                                                      CTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTG                                         >  SRR4302545.7543579/1‑94 (MQ=60)
                                                       TTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGT                                        <  SRR4302545.5398379/94‑1 (MQ=60)
                                                       TTTTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGT                                        >  SRR4302545.9176755/1‑94 (MQ=60)
                                                       TATTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGT                                        <  SRR4302545.3996010/94‑1 (MQ=60)
                                                        TTTTCATAACATTATTAAGCACATAACCGAACGTAAGTGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTT                                       <  SRR4302545.7640563/94‑1 (MQ=60)
                                                         TTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTC                                      >  SRR4302545.3858208/1‑94 (MQ=60)
                                                         TTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTC                                      <  SRR4302545.2439267/94‑1 (MQ=60)
                                                         TTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTC                                      >  SRR4302545.122884/1‑94 (MQ=60)
                                                         TTTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTC                                      <  SRR4302545.2031830/94‑1 (MQ=60)
                                                          TTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCA                                     <  SRR4302545.6671719/94‑1 (MQ=60)
                                                          TTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCA                                     >  SRR4302545.7923317/1‑94 (MQ=60)
                                                          TTCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCA                                     <  SRR4302545.8341543/94‑1 (MQ=60)
                                                           TCATAACATTATTAAGCACATACCCGAACGTAGGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGGTCAG                                    <  SRR4302545.6419240/94‑1 (MQ=60)
                                                           TCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAG                                    >  SRR4302545.3139716/1‑94 (MQ=60)
                                                           TCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAG                                    >  SRR4302545.1039342/1‑94 (MQ=60)
                                                           TCATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAG                                    <  SRR4302545.7727345/94‑1 (MQ=60)
                                                           TCATAACATTATTAAGCACATAACCGAACGTAAGCGCGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAG                                    <  SRR4302545.3070218/94‑1 (MQ=60)
                                                            CATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGT                                   >  SRR4302545.7024801/1‑94 (MQ=60)
                                                             ATAACATTATTAAGCACATACCCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTC                                  <  SRR4302545.6918296/94‑1 (MQ=60)
                                                             ATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTC                                  >  SRR4302545.5764837/1‑94 (MQ=60)
                                                             ATAACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTC                                  >  SRR4302545.741891/1‑94 (MQ=60)
                                                               AACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCT                                <  SRR4302545.4238987/94‑1 (MQ=60)
                                                               AACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCT                                <  SRR4302545.5529336/94‑1 (MQ=60)
                                                                ACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTC                               <  SRR4302545.699485/94‑1 (MQ=60)
                                                                ACATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTC                               >  SRR4302545.4532524/1‑94 (MQ=60)
                                                                ACATTATAAACCACATAACCGACCGTAAGCGGGAAAGTTGGGCGAACCCGCGAGAAACCTCTTGTTTTTACAAGAGCCCCCTTGTTCAGCCCTC                               <  SRR4302545.9547374/94‑1 (MQ=32)
                                                                  ATTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCTTCAG                             >  SRR4302545.9357111/1‑94 (MQ=60)
                                                                   TTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGT                            >  SRR4302545.10234806/1‑94 (MQ=60)
                                                                   TTATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGT                            <  SRR4302545.5627688/94‑1 (MQ=60)
                                                                    TATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTA                           <  SRR4302545.8579448/94‑1 (MQ=60)
                                                                    TATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTA                           >  SRR4302545.10434343/1‑94 (MQ=60)
                                                                     ATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAA                          <  SRR4302545.9459468/94‑1 (MQ=60)
                                                                     ATTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAA                          <  SRR4302545.6142605/94‑1 (MQ=60)
                                                                      TTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAATCTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAAC                         <  SRR4302545.6216862/94‑1 (MQ=60)
                                                                      TTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAAC                         <  SRR4302545.7165696/94‑1 (MQ=60)
                                                                      TTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAAC                         >  SRR4302545.6998658/1‑94 (MQ=60)
                                                                      TTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAAC                         <  SRR4302545.10736210/94‑1 (MQ=60)
                                                                      TTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAAC                         <  SRR4302545.2554652/94‑1 (MQ=60)
                                                                      TTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAAC                         >  SRR4302545.9391798/1‑94 (MQ=60)
                                                                      TTAAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAAC                         >  SRR4302545.5219907/1‑94 (MQ=60)
                                                                       TAAGCGCATAACCGAACGTAAGCGTAAAAGTCCGGCGAAGCCACGAGAAACCTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACT                        <  SRR4302545.705700/94‑1 (MQ=60)
                                                                        AAGCACATAACCGGACGTAAGCGTGAAAGCTAGGCGAGACCACGGGGAAACTCTTGTTATTAGAACAGAGCCCTTGTTCAGTCCTCAGTAACTG                       >  SRR4302545.7550243/1‑94 (MQ=32)
                                                                        AAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTG                       >  SRR4302545.4297294/1‑94 (MQ=60)
                                                                        AAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTG                       >  SRR4302545.5820907/1‑94 (MQ=60)
                                                                        AAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTG                       >  SRR4302545.3848653/1‑94 (MQ=60)
                                                                        AAGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTG                       >  SRR4302545.5820906/1‑94 (MQ=60)
                                                                         AGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGT                      <  SRR4302545.6441723/94‑1 (MQ=60)
                                                                         AGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGT                      <  SRR4302545.9229606/94‑1 (MQ=60)
                                                                         AGCACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGT                      <  SRR4302545.5236806/94‑1 (MQ=60)
                                                                           CACATAACCGAACGTAAGTGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAA                    >  SRR4302545.10307899/1‑94 (MQ=60)
                                                                            ACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAATCTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAAC                   <  SRR4302545.7966353/94‑1 (MQ=60)
                                                                            ACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAAC                   >  SRR4302545.10446657/1‑94 (MQ=60)
                                                                            ACATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAAC                   >  SRR4302545.2514153/1‑94 (MQ=60)
                                                                             CATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACC                  <  SRR4302545.1243563/94‑1 (MQ=60)
                                                                             CATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACC                  >  SRR4302545.2764074/1‑94 (MQ=60)
                                                                             CATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACC                  >  SRR4302545.8121800/1‑94 (MQ=60)
                                                                             CATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACC                  >  SRR4302545.4322539/1‑94 (MQ=60)
                                                                             CATAACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACC                  >  SRR4302545.10578786/1‑94 (MQ=60)
                                                                                AACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGC               >  SRR4302545.7088800/1‑94 (MQ=60)
                                                                                AACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGC               >  SRR4302545.7088807/1‑94 (MQ=60)
                                                                                AACCGAACGTAAGCGAGAAAGTTCGGCAAAACCACGAGAAAACTCTTGTTTTAACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGC               <  SRR4302545.8302592/94‑1 (MQ=60)
                                                                                AACCGAACCTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGC               <  SRR4302545.8302590/94‑1 (MQ=60)
                                                                                 ACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCT              <  SRR4302545.9425268/94‑1 (MQ=60)
                                                                                 ACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCT              <  SRR4302545.9735075/94‑1 (MQ=60)
                                                                                 ACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCT              <  SRR4302545.6778192/94‑1 (MQ=60)
                                                                                 ACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCT              >  SRR4302545.6485726/1‑94 (MQ=60)
                                                                                 ACCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCT              <  SRR4302545.4641925/94‑1 (MQ=60)
                                                                                  CCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTC             >  SRR4302545.160498/1‑94 (MQ=60)
                                                                                  CCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTC             <  SRR4302545.889064/94‑1 (MQ=60)
                                                                                  CCGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTC             >  SRR4302545.5506603/1‑94 (MQ=60)
                                                                                   CGAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCT            >  SRR4302545.6777172/1‑94 (MQ=60)
                                                                                    GAACGTAAGCGTGAAATTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGGCCTTGTTCAGTGCTCAGTAACTGTAACCAGCTCTT           >  SRR4302545.7766324/1‑94 (MQ=60)
                                                                                    GAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTT           >  SRR4302545.6769760/1‑94 (MQ=60)
                                                                                    GAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTT           >  SRR4302545.10227886/1‑94 (MQ=60)
                                                                                    GAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTT           >  SRR4302545.4233260/1‑94 (MQ=60)
                                                                                    GAACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTT           <  SRR4302545.7008937/94‑1 (MQ=60)
                                                                                     AACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTG          <  SRR4302545.855634/94‑1 (MQ=60)
                                                                                     AACGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGAGCCCTTGTTCAGTCCTCAGTAACTGTCACCAGCTCTTG          >  SRR4302545.3983847/1‑94 (MQ=60)
                                                                                       CGTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAA        >  SRR4302545.242925/1‑94 (MQ=60)
                                                                                        GTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAAT       <  SRR4302545.328878/94‑1 (MQ=60)
                                                                                        GTAAGCGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAAT       >  SRR4302545.5528124/1‑94 (MQ=60)
                                                                                             |                                                                                             
AGCACATGGATTTTCCAGCAGTGAATGCTGACGCTCCAACTGCGCAACCGGTTTCTTTTTCATAACATTATTAAGCACATAACCGAACGTAAGTGTGAAAGTTCGGCGAAACCACGAGAAAACTCTTGTTTTTACAAGAGCGCCCTTGTTCAGTCCTCAGTAACTGTAACCAGCTCTTGAATCCTGA  >  NC_000913/3084008‑3084194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑