Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,108,630 G→A R172W (CGG→TGG)  yghD ← putative secretion pathway protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,108,6300GA99.3% 1734.3 / ‑3.4 457R172W (CGG→TGG) yghDputative secretion pathway protein
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (268/186);  total (270/187)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

Only 100 of 465 total aligned reads displayed.

AAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATATTTTTCATCCAGTGCGTTTAACCA  >  NC_000913/3108539‑3108720
                                                                                           |                                                                                          
  aGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGc                                                                                        <  2:2844612/94‑1 (MQ=255)
   gTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGca                                                                                       <  1:7057847/94‑1 (MQ=255)
    tCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTCCCCCCGTCCAAACTCCAGCCACTTcac                                                                                      <  1:7385935/94‑1 (MQ=255)
      tGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACAt                                                                                    <  2:2622888/94‑1 (MQ=255)
      tGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCACCGTACAAACTCCAGCCACTGCACAt                                                                                    >  2:1461085/1‑94 (MQ=255)
         gggAATTCCTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCa                                                                                 <  1:884631/94‑1 (MQ=255)
          ggAATTTCTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATATTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCAc                                                                                <  2:9052863/94‑1 (MQ=255)
           gAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCAcc                                                                               >  2:8982074/1‑94 (MQ=255)
           gAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCAcc                                                                               >  1:1913283/1‑94 (MQ=255)
            aaTTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCa                                                                              >  2:5561337/1‑94 (MQ=255)
              ttACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATc                                                                            <  2:4301308/94‑1 (MQ=255)
              ttACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATc                                                                            <  1:4198991/94‑1 (MQ=255)
               tACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATcc                                                                           >  2:163528/1‑94 (MQ=255)
                tctctCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATccc                                                                          <  1:2516057/93‑1 (MQ=255)
                aCTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATccc                                                                          <  2:3900386/94‑1 (MQ=255)
                 ctctCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCa                                                                         <  2:6914717/94‑1 (MQ=255)
                  tctcGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAg                                                                        >  1:9688707/1‑94 (MQ=255)
                  tctcGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAg                                                                        <  1:8245693/94‑1 (MQ=255)
                  tctcGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAg                                                                        <  2:7850847/94‑1 (MQ=255)
                  tctcGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAg                                                                        <  2:3683382/94‑1 (MQ=255)
                    tcGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGc                                                                      <  1:8079203/94‑1 (MQ=255)
                      gCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCtt                                                                    >  2:4925056/1‑94 (MQ=255)
                      gCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCtt                                                                    <  1:3279556/94‑1 (MQ=255)
                        cACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTtct                                                                  >  2:1053358/1‑94 (MQ=255)
                        cACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTtct                                                                  <  1:4608350/94‑1 (MQ=255)
                        cACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTtct                                                                  >  2:9376159/1‑94 (MQ=255)
                        cACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTtct                                                                  <  2:7738316/94‑1 (MQ=255)
                        cACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTtct                                                                  >  1:4762044/1‑94 (MQ=255)
                        cACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTtct                                                                  >  2:5293490/1‑94 (MQ=255)
                         aCGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTtctc                                                                 <  2:416166/94‑1 (MQ=255)
                          cGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAACCTCCAGCCACTGCACATTCACCATCCCAGGCTtctcg                                                                <  2:3347384/94‑2 (MQ=255)
                          cGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTca                                                                <  1:8437684/94‑1 (MQ=255)
                          cGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTca                                                                <  2:1838029/94‑1 (MQ=255)
                           gTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCATCCACTGCACATTCACCATCCCAGGCTTCTcag                                                               >  2:2208139/1‑94 (MQ=255)
                            ttAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTcagc                                                              <  1:1024739/94‑1 (MQ=255)
                             tAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTcagca                                                             >  2:9566633/1‑94 (MQ=255)
                             tAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTcagca                                                             >  1:8324781/1‑94 (MQ=255)
                              aaCGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTcaggag                                                            >  1:5048195/1‑94 (MQ=255)
                              aaCGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTcagcag                                                            >  1:5972078/1‑94 (MQ=255)
                              aaCGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTcagcag                                                            >  1:1072399/1‑94 (MQ=255)
                              aaCGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTcagcag                                                            >  2:6810970/1‑94 (MQ=255)
                              aaCGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTcagcag                                                            >  2:10435580/1‑94 (MQ=255)
                                cGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAgcagca                                                          <  1:6649034/94‑1 (MQ=255)
                                cGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAgcagca                                                          >  2:1251934/1‑94 (MQ=255)
                                 gagaGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCAc                                                         >  2:6063896/1‑94 (MQ=255)
                                 gagaGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCAc                                                         <  1:5839341/94‑1 (MQ=255)
                                   gagTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTg                                                       <  2:7238873/94‑1 (MQ=255)
                                    agTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGa                                                      >  2:8638225/1‑94 (MQ=255)
                                      tAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACa                                                    <  1:3694538/94‑1 (MQ=255)
                                        aTTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATc                                                  >  1:3458187/1‑94 (MQ=255)
                                         ttttATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCa                                                 <  2:8370310/94‑1 (MQ=255)
                                           ttATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAAt                                               <  1:5623081/94‑1 (MQ=255)
                                            tATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAAtt                                              >  1:3028673/1‑94 (MQ=255)
                                            tATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAAtt                                              >  1:391346/1‑94 (MQ=255)
                                             aTTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAAttt                                             <  2:6501874/94‑1 (MQ=255)
                                                gATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTtgtg                                          >  1:2234215/1‑94 (MQ=255)
                                                gATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTtgtg                                          <  2:7615477/94‑1 (MQ=255)
                                                gATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTtgtg                                          <  2:5861652/94‑1 (MQ=255)
                                                 atatTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTgtgt                                         >  1:1428423/1‑94 (MQ=255)
                                                  tatTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTc                                        <  2:6249066/94‑1 (MQ=255)
                                                  tatTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTc                                        >  1:7835637/1‑94 (MQ=255)
                                                  tatTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTc                                        >  2:8002765/1‑94 (MQ=255)
                                                    ttAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCAAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCAc                                      >  1:10179969/1‑94 (MQ=255)
                                                     tAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCAcc                                     <  1:6231789/94‑1 (MQ=255)
                                                     tAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCAcc                                     >  2:8850803/1‑94 (MQ=255)
                                                       aTCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCAcccc                                   >  1:5475141/1‑93 (MQ=255)
                                                       aTCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCAccca                                   <  1:3542373/94‑2 (MQ=255)
                                                        tctcCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCATTGACATCAATTTGTGTCACCCGc                                  >  1:7585046/1‑94 (MQ=255)
                                                         ctcCTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCa                                 <  1:4929367/94‑1 (MQ=255)
                                                           ccTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAgc                               <  2:7429699/94‑1 (MQ=255)
                                                           ccTGATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCACAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAgc                               >  2:9835232/1‑94 (MQ=255)
                                                              gATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCa                            >  1:10005421/1‑94 (MQ=255)
                                                              gATACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCa                            >  2:241595/1‑94 (MQ=255)
                                                               aTACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCat                           <  1:2759299/94‑1 (MQ=255)
                                                                tACTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCata                          >  2:10034322/1‑94 (MQ=255)
                                                                 aCTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCatat                         <  2:4856552/94‑1 (MQ=255)
                                                                 aCTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCatat                         >  1:813734/1‑94 (MQ=255)
                                                                  cTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATAtt                        <  2:176489/94‑1 (MQ=255)
                                                                  cTTTACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATAtt                        >  2:6629219/1‑94 (MQ=255)
                                                                   tttACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATAttt                       >  2:10296590/1‑94 (MQ=255)
                                                                    ttACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCCGCGCATAtttt                      >  1:8994952/1‑94 (MQ=255)
                                                                    ttACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATAtttt                      >  2:4672208/1‑94 (MQ=255)
                                                                    ttACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATAtttt                      <  2:6387786/94‑1 (MQ=255)
                                                                    ttACCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATAtttt                      >  2:5058076/1‑94 (MQ=255)
                                                                      aCCCCCGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATATTTTTc                    <  1:6369324/94‑1 (MQ=255)
                                                                       cccccGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATATTTTTCa                   >  1:1042474/1‑94 (MQ=255)
                                                                         cccGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATATTTTTCATc                 <  2:3019066/94‑1 (MQ=255)
                                                                           cGTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATATTTTTCATCCa               >  2:1960709/1‑94 (MQ=255)
                                                                            gTCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATATTTTTCATCCAg              >  2:1071919/1‑94 (MQ=255)
                                                                             tCCAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATATTTTTCATCCAGt             <  1:9435785/94‑1 (MQ=255)
                                                                              ccAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATATTTTTCATCCAGTg            <  2:6974301/94‑1 (MQ=255)
                                                                              ccAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATATTTTTCATCCAGTg            >  1:9860786/1‑94 (MQ=255)
                                                                              ccAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATATTTTTCATCCAGTg            <  1:9250085/94‑1 (MQ=255)
                                                                              ccAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATATTTTTCATCCAGTg            >  2:8127484/1‑94 (MQ=255)
                                                                               cAAACTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATATTTTTCATCCAGTGc           >  2:10174084/1‑94 (MQ=255)
                                                                                  aCTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATATTTTTCATCCAGTGCGtt        <  1:6970506/94‑1 (MQ=255)
                                                                                   cTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATATTTTTCATCCAGTGCGttt       <  1:7220143/94‑1 (MQ=255)
                                                                                   cTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATATTTTTCATCCAGTGCGttt       >  1:444220/1‑94 (MQ=255)
                                                                                   cTCCAGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATATTTTTCATCCAGTGCGttt       >  1:1659204/1‑94 (MQ=255)
                                                                                       aGCCACTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATATTTTTCATCCAGTGCGTTTAAcc   >  1:2340294/1‑94 (MQ=255)
                                                                                           |                                                                                          
AAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATATTTTTCATCCAGTGCGTTTAACCA  >  NC_000913/3108539‑3108720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 233 total aligned reads displayed.
TTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTT‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACC  >  NC_000913/3115024‑3115209
                                                                                              |                                                                                             
TTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTT‑CC                                                                                              <  SRR4302545.3282384/94‑1 (MQ=60)
 TTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTT‑Cct                                                                                             <  SRR4302545.3116458/94‑3 (MQ=60)
 TTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTT‑Cct                                                                                             <  SRR4302545.2309308/94‑3 (MQ=60)
 TTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTT‑Cct                                                                                             <  SRR4302545.9297748/94‑3 (MQ=60)
   GCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGC                                                                                            >  SRR4302545.5495933/1‑94 (MQ=60)
   GCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGC                                                                                            <  SRR4302545.5782272/94‑1 (MQ=60)
    CTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCG                                                                                           >  SRR4302545.3261049/1‑94 (MQ=60)
    CTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCG                                                                                           >  SRR4302545.9788966/1‑94 (MQ=60)
    CTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGGCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTT‑CTGCG                                                                                           >  SRR4302545.4250347/1‑94 (MQ=60)
     TGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGG                                                                                          >  SRR4302545.3375879/1‑94 (MQ=60)
     TGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGG                                                                                          <  SRR4302545.10713021/94‑1 (MQ=60)
     TGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGG                                                                                          <  SRR4302545.2512853/94‑1 (MQ=60)
     TGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGG                                                                                          >  SRR4302545.8076726/1‑94 (MQ=60)
      GGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCATCCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGA                                                                                         <  SRR4302545.5582439/94‑1 (MQ=60)
        TATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATC                                                                                       >  SRR4302545.8174749/1‑94 (MQ=60)
        TATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATC                                                                                       <  SRR4302545.7316456/94‑1 (MQ=60)
        TATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATC                                                                                       <  SRR4302545.10261024/94‑1 (MQ=60)
        TATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATC                                                                                       <  SRR4302545.5327502/94‑1 (MQ=60)
        TATCTACACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATC                                                                                       <  SRR4302545.7316457/94‑1 (MQ=60)
         ATCTGCCCGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATACAAAGCCGGTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCG                                                                                      <  SRR4302545.5312252/94‑1 (MQ=60)
          TCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGA                                                                                     <  SRR4302545.7019272/94‑1 (MQ=60)
          TCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGA                                                                                     <  SRR4302545.5321797/94‑1 (MQ=60)
          TCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGA                                                                                     <  SRR4302545.8568658/94‑1 (MQ=60)
           CTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAG                                                                                    >  SRR4302545.413335/1‑94 (MQ=60)
           CTGCACGCAGCGGGATTGCATAAGGATCGTGACCCACCTGAGTCACATATTCAAAGCCGTTAAGCATCAGCAGCGGCA‑TTT‑Cccgcggctctag                                                                                   >  SRR4302545.9499522/1‑82 (MQ=60)
            TGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGA                                                                                   <  SRR4302545.4268902/94‑1 (MQ=60)
            TGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGA                                                                                   >  SRR4302545.9828265/1‑94 (MQ=60)
              CACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATC                                                                                 <  SRR4302545.10908689/94‑1 (MQ=60)
                 GCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGATAAGGATCAGCAGCGGCA‑TTTACTGCGGATCGCGATCGCC                                                                              >  SRR4302545.4301472/1‑94 (MQ=60)
                  CATCGGGATTGCATAAGGATCGATACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGtga                                                                             >  SRR4302545.4453700/1‑91 (MQ=60)
                  CAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCA                                                                             >  SRR4302545.4781508/1‑94 (MQ=60)
                  CAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCA                                                                             <  SRR4302545.1843523/94‑1 (MQ=60)
                  CAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCAGCA‑TTTCCTGCGGATCGAGATCCCCA                                                                             >  SRR4302545.3275985/1‑94 (MQ=60)
                   AGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCAT                                                                            >  SRR4302545.1449414/1‑94 (MQ=60)
                   AGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCAT                                                                            <  SRR4302545.1909381/94‑1 (MQ=60)
                   AGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCAT                                                                            <  SRR4302545.7308700/94‑1 (MQ=60)
                   AGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCAT                                                                            >  SRR4302545.3507741/1‑94 (MQ=60)
                     CGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAG                                                                          <  SRR4302545.18148/94‑1 (MQ=60)
                      GGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGC                                                                         >  SRR4302545.8015115/1‑94 (MQ=60)
                      GGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGC                                                                         >  SRR4302545.7170689/1‑94 (MQ=60)
                      GGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGC                                                                         >  SRR4302545.6047583/1‑94 (MQ=60)
                      GGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGC                                                                         >  SRR4302545.5909275/1‑94 (MQ=60)
                      GGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGC                                                                         >  SRR4302545.5595653/1‑94 (MQ=60)
                      GGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGC                                                                         >  SRR4302545.2257052/1‑94 (MQ=60)
                       GGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCT                                                                        <  SRR4302545.2622696/94‑1 (MQ=60)
                        GATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTA                                                                       <  SRR4302545.1094209/94‑1 (MQ=60)
                        GATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTA                                                                       >  SRR4302545.7697070/1‑94 (MQ=60)
                        GATGGCATAAGGATCTTTA‑CCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCACAGCTAC                                                                      <  SRR4302545.7004535/94‑1 (MQ=60)
                         ATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTAC                                                                      >  SRR4302545.1795074/1‑94 (MQ=60)
                         ATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTAC                                                                      >  SRR4302545.1039543/1‑94 (MQ=60)
                         ATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTAC                                                                      >  SRR4302545.7317217/1‑94 (MQ=60)
                         ATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTAC                                                                      >  SRR4302545.6398853/1‑94 (MQ=60)
                           TGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTT                                                                    <  SRR4302545.2331932/94‑1 (MQ=60)
                           TGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTT                                                                    <  SRR4302545.8941761/94‑1 (MQ=60)
                              ATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAA                                                                 <  SRR4302545.4436124/94‑1 (MQ=60)
                              ATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAA                                                                 <  SRR4302545.4414729/94‑1 (MQ=60)
                              ATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAA                                                                 <  SRR4302545.4436123/94‑1 (MQ=60)
                               TAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAAT                                                                <  SRR4302545.9638928/94‑1 (MQ=60)
                               TAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAAT                                                                <  SRR4302545.3075822/94‑1 (MQ=60)
                               TAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAAT                                                                >  SRR4302545.4420261/1‑94 (MQ=60)
                               TAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAAT                                                                >  SRR4302545.4420260/1‑94 (MQ=60)
                               TAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAAT                                                                <  SRR4302545.1068737/94‑1 (MQ=60)
                               TAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAAT                                                                >  SRR4302545.8425500/1‑94 (MQ=60)
                                AAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATG                                                               >  SRR4302545.5191104/1‑94 (MQ=60)
                                AAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATG                                                               >  SRR4302545.8436363/1‑94 (MQ=60)
                                AAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATG                                                               <  SRR4302545.5063049/94‑1 (MQ=60)
                                 AGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGC                                                              >  SRR4302545.4041469/1‑94 (MQ=60)
                                 AGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGC                                                              >  SRR4302545.1388766/1‑94 (MQ=60)
                                 AGGATCGTTACCCACCTGAGACACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGGGATCGCCATAGCTACTTAAATGC                                                              >  SRR4302545.8846780/1‑94 (MQ=60)
                                  GGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCT                                                             >  SRR4302545.10605941/1‑94 (MQ=60)
                                  GGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCT                                                             >  SRR4302545.8056952/1‑94 (MQ=60)
                                   GATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTC                                                            <  SRR4302545.8613621/94‑1 (MQ=60)
                                   GATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTC                                                            <  SRR4302545.94637/94‑1 (MQ=60)
                                    ATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCA                                                           >  SRR4302545.5782316/1‑94 (MQ=60)
                                    ATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCA                                                           <  SRR4302545.1448677/94‑1 (MQ=60)
                                     TCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGGTCGAGATCGCCATAGCTACTTAGATGCTCAA                                                          >  SRR4302545.3226033/1‑94 (MQ=60)
                                     TCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAA                                                          >  SRR4302545.8171324/1‑94 (MQ=60)
                                      CGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAC                                                         <  SRR4302545.9206649/94‑1 (MQ=60)
                                        TTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAG                                                       >  SRR4302545.2905760/1‑94 (MQ=60)
                                        TTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAG                                                       <  SRR4302545.2373545/94‑1 (MQ=60)
                                        TTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAG                                                       >  SRR4302545.5110411/1‑94 (MQ=60)
                                         TACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGA                                                      <  SRR4302545.2194691/94‑1 (MQ=60)
                                         TACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGA                                                      >  SRR4302545.3517276/1‑94 (MQ=60)
                                         TACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGA                                                      <  SRR4302545.7004460/94‑1 (MQ=60)
                                         TACCCACCTGAGTCACATATTCAAAGCCGATAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGA                                                      <  SRR4302545.8006960/94‑1 (MQ=60)
                                          ACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAG                                                     <  SRR4302545.10804413/94‑1 (MQ=60)
                                           CCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGA                                                    >  SRR4302545.7589011/1‑94 (MQ=60)
                                            CCTCCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCAGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAT                                                   <  SRR4302545.7201130/94‑1 (MQ=60)
                                            CCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAT                                                   <  SRR4302545.9555393/94‑1 (MQ=60)
                                            CCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAT                                                   <  SRR4302545.5956569/94‑1 (MQ=60)
                                            CCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAT                                                   <  SRR4302545.654644/94‑1 (MQ=60)
                                            CCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAT                                                   >  SRR4302545.10026817/1‑94 (MQ=60)
                                            CCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAT                                                   >  SRR4302545.2974104/1‑94 (MQ=60)
                                            CCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAT                                                   <  SRR4302545.3324606/94‑1 (MQ=60)
                                            CCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAT                                                   >  SRR4302545.906456/1‑94 (MQ=60)
                                            CCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAT                                                   <  SRR4302545.4023471/94‑1 (MQ=60)
                                             CACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATG                                                  >  SRR4302545.8196214/1‑94 (MQ=60)
                                               CCTGAGTCACATCTTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCC                                                >  SRR4302545.3942157/1‑94 (MQ=60)
                                               CCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCC                                                <  SRR4302545.3072872/94‑1 (MQ=60)
                                               CCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCC                                                <  SRR4302545.6124281/94‑1 (MQ=60)
                                               CCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCC                                                <  SRR4302545.2151297/94‑1 (MQ=60)
                                                CTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAagccg                                               >  SRR4302545.2645267/1‑89 (MQ=60)
                                                CTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCC                                               <  SRR4302545.4239174/94‑1 (MQ=60)
                                                CTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCC                                               <  SRR4302545.8144615/94‑1 (MQ=60)
                                                CTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCC                                               <  SRR4302545.7568927/94‑1 (MQ=60)
                                                 TGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCG                                              <  SRR4302545.5988372/94‑1 (MQ=60)
                                                 TGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCACAGCTACTTAAATGCTCAACAGAGATGCCCG                                              >  SRR4302545.10656612/1‑94 (MQ=60)
                                                 CGAGTCACATATTCAAAGCCGTTAAGGATCAGAAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCG                                              <  SRR4302545.5331820/94‑1 (MQ=60)
                                                   AGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCA                                            >  SRR4302545.5042654/1‑94 (MQ=60)
                                                    GTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAA                                           <  SRR4302545.2927209/94‑1 (MQ=60)
                                                     TCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAA                                          >  SRR4302545.5676060/1‑94 (MQ=60)
                                                     TCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAA                                          <  SRR4302545.3712090/94‑1 (MQ=60)
                                                     TCACATATTCAAAGACGTTAAGGATCAGCAGCGGCAGTTC‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAA                                          <  SRR4302545.3712091/94‑1 (MQ=60)
                                                       ACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAAT                                        >  SRR4302545.1145953/1‑94 (MQ=60)
                                                       ACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAAT                                        >  SRR4302545.7288095/1‑94 (MQ=60)
                                                       ACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAAT                                        <  SRR4302545.9369718/94‑1 (MQ=60)
                                                        CATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATC                                       >  SRR4302545.4022320/1‑94 (MQ=60)
                                                        CATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATC                                       >  SRR4302545.7956737/1‑94 (MQ=60)
                                                         ATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCC                                      <  SRR4302545.2497927/94‑1 (MQ=60)
                                                         ATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCC                                      <  SRR4302545.7768988/94‑1 (MQ=60)
                                                         ATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCC                                      >  SRR4302545.2537702/1‑94 (MQ=60)
                                                          TATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCG                                     <  SRR4302545.9358160/94‑1 (MQ=60)
                                                          TATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCG                                     >  SRR4302545.694609/1‑94 (MQ=60)
                                                          TATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCG                                     >  SRR4302545.8463663/1‑94 (MQ=60)
                                                          TATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCG                                     <  SRR4302545.4232272/94‑1 (MQ=60)
                                                            TTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGA                                   <  SRR4302545.7819277/94‑1 (MQ=60)
                                                             TCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGAT                                  <  SRR4302545.5224029/94‑1 (MQ=60)
                                                              CAAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATG                                 <  SRR4302545.6601346/94‑1 (MQ=60)
                                                               AAAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGG                                >  SRR4302545.7000396/1‑94 (MQ=60)
                                                                AAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGA                               >  SRR4302545.79021/1‑94 (MQ=60)
                                                                AAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGA                               <  SRR4302545.9820758/94‑1 (MQ=60)
                                                                AAGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGA                               <  SRR4302545.3159143/94‑1 (MQ=60)
                                                                 AGCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAA                              <  SRR4302545.8988071/94‑1 (MQ=60)
                                                                  GCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAG                             <  SRR4302545.902699/94‑1 (MQ=60)
                                                                  GCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAG                             <  SRR4302545.10056047/94‑1 (MQ=60)
                                                                  GCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAG                             <  SRR4302545.2384626/94‑1 (MQ=60)
                                                                  GCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAG                             >  SRR4302545.2245908/1‑94 (MQ=60)
                                                                  GCCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAG                             <  SRR4302545.3906909/94‑1 (MQ=60)
                                                                   CCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGT                            <  SRR4302545.274191/94‑1 (MQ=60)
                                                                   CCGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGT                            <  SRR4302545.2241447/94‑1 (MQ=60)
                                                                   CCGTTAAGGAGCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGGTGCCAGCAAAATCCGGATGGAAGT                            <  SRR4302545.7649589/94‑1 (MQ=60)
                                                                    CGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTC                           <  SRR4302545.696811/94‑1 (MQ=60)
                                                                    CGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTC                           >  SRR4302545.1979454/1‑94 (MQ=60)
                                                                    CGTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTC                           <  SRR4302545.7241729/94‑1 (MQ=60)
                                                                    CGTGAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTC                           <  SRR4302545.7780834/94‑1 (MQ=60)
                                                                     GTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCG                          <  SRR4302545.9291675/94‑1 (MQ=60)
                                                                     GTTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCG                          >  SRR4302545.9410431/1‑94 (MQ=60)
                                                                      TTAAGGCTCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAGAATCCGGATGGAAGTCGA                         <  SRR4302545.9698566/94‑1 (MQ=60)
                                                                      TTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGA                         >  SRR4302545.2435251/1‑94 (MQ=60)
                                                                      TTAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGA                         >  SRR4302545.9722707/1‑94 (MQ=60)
                                                                       TAAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAA                        <  SRR4302545.2535659/94‑1 (MQ=60)
                                                                        AAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAAC                       <  SRR4302545.9643191/94‑1 (MQ=60)
                                                                        AAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAAC                       <  SRR4302545.7625048/94‑1 (MQ=60)
                                                                        AAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAAC                       >  SRR4302545.6365239/1‑94 (MQ=60)
                                                                        AAGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAAC                       >  SRR4302545.4181964/1‑94 (MQ=60)
                                                                         AGGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACG                      >  SRR4302545.5860064/1‑94 (MQ=60)
                                                                          GGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGC                     <  SRR4302545.6818334/94‑1 (MQ=60)
                                                                          GGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGC                     >  SRR4302545.3512032/1‑94 (MQ=60)
                                                                          GGATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGC                     >  SRR4302545.9184431/1‑94 (MQ=60)
                                                                           GATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCA                    >  SRR4302545.8780651/1‑94 (MQ=60)
                                                                           GATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCA                    <  SRR4302545.1029724/94‑1 (MQ=60)
                                                                            ATCAGGAGCGGCA‑TTTCCTGCGGATCGAGATCCCCATAGCTACCTAAATGCTAAACAGAGATGCCCGCAAAATCCGGAGGGAAGTCGAACGCAG                   <  SRR4302545.8093230/94‑1 (MQ=60)
                                                                            ATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAG                   >  SRR4302545.5373518/1‑94 (MQ=60)
                                                                            ATCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAG                   <  SRR4302545.1433749/94‑1 (MQ=60)
                                                                             TCAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGC                  <  SRR4302545.6074247/94‑1 (MQ=60)
                                                                              CAGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCG                 <  SRR4302545.3324290/94‑1 (MQ=60)
                                                                               AGCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGC                >  SRR4302545.2863491/1‑94 (MQ=60)
                                                                               AGCAGCGGCA‑TTTCCTGCGGATCGAGAGCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGGCGAACGCAGCGC                >  SRR4302545.9685847/1‑94 (MQ=60)
                                                                                GCAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCT               <  SRR4302545.5698481/94‑1 (MQ=60)
                                                                                 CAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTG              >  SRR4302545.8055743/1‑94 (MQ=60)
                                                                                 CAGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTG              <  SRR4302545.1726407/94‑1 (MQ=60)
                                                                                  AGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCTGGATGGAAGTCGAACGCAGCGCCGT             >  SRR4302545.9883194/1‑94 (MQ=60)
                                                                                  AGCGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGT             >  SRR4302545.2891683/1‑94 (MQ=60)
                                                                                    CGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTT           >  SRR4302545.307556/1‑94 (MQ=60)
                                                                                    CGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTT           <  SRR4302545.1423705/94‑1 (MQ=60)
                                                                                    CGGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTT           >  SRR4302545.6036798/1‑94 (MQ=60)
                                                                                     GGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTC          <  SRR4302545.3420399/94‑1 (MQ=60)
                                                                                     GGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTC          >  SRR4302545.2857203/1‑94 (MQ=60)
                                                                                     GGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTC          >  SRR4302545.5604389/1‑94 (MQ=60)
                                                                                     GGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTC          >  SRR4302545.925542/1‑94 (MQ=60)
                                                                                     GGCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTA          <  SRR4302545.9239905/94‑1 (MQ=60)
                                                                                      GCA‑TTTCCTGCGGATCGAGATCTCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCC         >  SRR4302545.6945530/1‑94 (MQ=60)
                                                                                      GCA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCC         <  SRR4302545.3541650/94‑1 (MQ=60)
                                                                                       CA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGACCGCAGCGCTGTTTCCT        <  SRR4302545.1664111/94‑1 (MQ=60)
                                                                                       CA‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCT        >  SRR4302545.365978/1‑94 (MQ=60)
                                                                                        A‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTG       <  SRR4302545.685717/94‑1 (MQ=60)
                                                                                        A‑TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTG       >  SRR4302545.4731958/1‑94 (MQ=60)
                                                                                          TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGT      <  SRR4302545.863555/94‑1 (MQ=60)
                                                                                          TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGT      >  SRR4302545.2399616/1‑94 (MQ=60)
                                                                                          TTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGT      <  SRR4302545.5075687/94‑1 (MQ=60)
                                                                                          TTC‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTA     >  SRR4302545.2116599/1‑94 (MQ=60)
                                                                                          TTC‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTA     <  SRR4302545.9598096/94‑1 (MQ=60)
                                                                                           TC‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCGACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAA    <  SRR4302545.5567484/94‑1 (MQ=60)
                                                                                           TC‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAA    <  SRR4302545.9884245/94‑1 (MQ=60)
                                                                                           TC‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAA    <  SRR4302545.5795088/94‑1 (MQ=60)
                                                                                           TC‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAA    >  SRR4302545.3792876/1‑94 (MQ=60)
                                                                                            C‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAAC   >  SRR4302545.4698673/1‑94 (MQ=60)
                                                                                              CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACC  >  SRR4302545.983975/1‑94 (MQ=60)
                                                                                              CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACC  <  SRR4302545.3611311/94‑1 (MQ=60)
                                                                                              CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACC  <  SRR4302545.3451270/94‑1 (MQ=60)
                                                                                              |                                                                                             
TTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCA‑TTT‑CTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACC  >  NC_000913/3115024‑3115209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑