Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,127,865 T→C A122A (GCA→GCG)  b2981 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,127,8650TC99.4% 1599.5 / ‑1.8 465A122A (GCA→GCG) b2981hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (259/203);  total (260/205)
Fisher's exact test for biased strand distribution p-value = 5.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 507 total aligned reads displayed.

CTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCTGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCGGTGCAAG  >  NC_000913/3127774‑3127958
                                                                                           |                                                                                             
cTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCcgccgc                                                                                             <  1:6200521/94‑1 (MQ=255)
cTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCcgccgc                                                                                             <  2:3767748/94‑1 (MQ=255)
 ttCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCcgcg                                                                                            >  2:1953714/1‑94 (MQ=255)
 ttCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCcgcg                                                                                            >  2:8563270/1‑94 (MQ=255)
 ttCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCcgcg                                                                                            <  1:3640316/94‑1 (MQ=255)
  tCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCTCCgcgc                                                                                           >  2:4543723/1‑94 (MQ=255)
  tCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCgcgc                                                                                           >  1:3062848/1‑94 (MQ=255)
   cAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGcc                                                                                          <  2:4392088/94‑1 (MQ=255)
    aaTCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCa                                                                                         >  2:1419876/1‑94 (MQ=255)
    aaTCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCa                                                                                         <  2:8097931/94‑1 (MQ=255)
      tCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCaaa                                                                                       <  2:2701848/94‑1 (MQ=255)
       cAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAaac                                                                                      >  2:6183513/1‑94 (MQ=255)
        aGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAaaca                                                                                     >  1:1257689/1‑94 (MQ=255)
             tCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAAcgcg                                                                                <  1:4265957/94‑1 (MQ=255)
             tCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAAcgcg                                                                                <  2:7405828/94‑1 (MQ=255)
               cGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGc                                                                              >  1:10300357/1‑94 (MQ=255)
                 cTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCaa                                                                            <  2:6683853/94‑1 (MQ=255)
                 cTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCaa                                                                            >  1:3557795/1‑94 (MQ=255)
                 cTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCaa                                                                            <  1:971512/94‑1 (MQ=255)
                    tCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCaaaaa                                                                         >  1:4142368/1‑94 (MQ=255)
                     ccTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAAc                                                                        >  2:2131386/1‑94 (MQ=255)
                      cTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAAcc                                                                       <  1:144600/94‑1 (MQ=255)
                      cTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAAcc                                                                       >  2:906387/1‑94 (MQ=255)
                      cTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTGGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAAcc                                                                       <  1:575190/94‑1 (MQ=255)
                       tgattgatATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCt                                                                      <  2:1874712/94‑1 (MQ=255)
                       tgattgatATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCt                                                                      <  1:10086007/94‑1 (MQ=255)
                       tgattgatATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCt                                                                      >  2:10360451/1‑94 (MQ=255)
                        gattgatATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTg                                                                     <  1:7921251/94‑1 (MQ=255)
                         attgatATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGc                                                                    <  2:3412411/94‑1 (MQ=255)
                         attgatATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGc                                                                    >  2:7832215/1‑94 (MQ=255)
                           tgatATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCgg                                                                  >  1:1535796/1‑94 (MQ=255)
                           tgatATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCgg                                                                  >  1:6364436/1‑94 (MQ=255)
                             atatTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGat                                                                >  1:1713557/1‑94 (MQ=255)
                              tatTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGatc                                                               >  2:6267644/1‑94 (MQ=255)
                              tatTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGatc                                                               >  2:1540161/1‑94 (MQ=255)
                              tatTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGatc                                                               <  2:8353463/94‑1 (MQ=255)
                               atTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGatca                                                              >  2:3113516/1‑94 (MQ=255)
                               atTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGatca                                                              >  2:1027898/1‑94 (MQ=255)
                               atTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGatca                                                              <  1:9327704/94‑1 (MQ=255)
                               atTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGatca                                                              <  1:2751905/94‑1 (MQ=255)
                                ttCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGatcat                                                             <  1:3785261/94‑1 (MQ=255)
                                  cAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGAtcatca                                                           >  1:5030553/1‑94 (MQ=255)
                                   aGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCaa                                                          >  1:8891367/1‑94 (MQ=255)
                                   aGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCaa                                                          >  1:3189830/1‑94 (MQ=255)
                                    ggCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAAt                                                         <  2:1052206/94‑1 (MQ=255)
                                    ggCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAAt                                                         >  2:2330839/1‑94 (MQ=255)
                                     gcgcaaaGGGCCGCTGAACTTACTTGCACCTTGTGACTTCAACCACCCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATg                                                        <  2:697338/90‑1 (MQ=255)
                                     gCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATg                                                        >  1:8111465/1‑94 (MQ=255)
                                     gCTGAAAGCACCGCTGATCTTACTTGCACCTTGTGACTTCACCCACGCTCCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATg                                                        <  1:5788104/94‑1 (MQ=255)
                                        gAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCg                                                     <  2:5594133/94‑1 (MQ=255)
                                        gAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCg                                                     <  2:3531181/94‑1 (MQ=255)
                                          aaGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTc                                                   >  2:5366225/1‑94 (MQ=255)
                                               ccGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCa                                              <  1:4699236/94‑1 (MQ=255)
                                               ccGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCa                                              <  1:4514982/94‑1 (MQ=255)
                                               ccGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCa                                              <  1:8474121/94‑1 (MQ=255)
                                                 gCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATg                                            <  2:5323569/94‑1 (MQ=255)
                                                 gCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATg                                            <  2:936416/94‑1 (MQ=255)
                                                 acTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATg                                            >  2:9590960/2‑94 (MQ=255)
                                                  cTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGc                                           >  2:1638612/1‑94 (MQ=255)
                                                  cTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGc                                           >  2:3960451/1‑94 (MQ=255)
                                                  cTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGc                                           <  2:3053751/94‑1 (MQ=255)
                                                   tGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGcc                                          <  1:9522679/94‑1 (MQ=255)
                                                   tGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGcc                                          >  2:482430/1‑94 (MQ=255)
                                                   tGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGcc                                          >  1:3903167/1‑94 (MQ=255)
                                                   tGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGcc                                          >  1:4880978/1‑94 (MQ=255)
                                                      tCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGaa                                       >  2:7579713/1‑94 (MQ=255)
                                                       cTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCACAAACCTGCGGATCATCAATGGCGTCAATCATGCCGaaa                                      >  2:4675736/1‑94 (MQ=255)
                                                       cTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGaaa                                      <  2:8961870/94‑1 (MQ=255)
                                                       cTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGaaa                                      >  2:7248336/1‑94 (MQ=255)
                                                       cTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGaaa                                      >  1:777286/1‑94 (MQ=255)
                                                        ttACTTGCACCTTGTGATTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACGTGCGGATCATCAATGGCGTCAATCATGCCGAAAt                                     <  1:8216591/94‑1 (MQ=255)
                                                        ttACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAAt                                     >  2:3243860/1‑94 (MQ=255)
                                                        ttACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAAt                                     >  1:2657004/1‑94 (MQ=255)
                                                        ttACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAAt                                     <  2:4126905/94‑1 (MQ=255)
                                                          aCTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGGTCATCAATGGCGTCAATCATGCCGAAATGa                                   <  1:5525261/94‑1 (MQ=255)
                                                          aCTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGa                                   >  2:7130904/1‑94 (MQ=255)
                                                            ttGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGAcc                                 >  2:5924710/1‑94 (MQ=255)
                                                            ttGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGAcc                                 >  2:4922319/1‑94 (MQ=255)
                                                               cACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGt                              >  1:10162751/1‑94 (MQ=255)
                                                               cACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGt                              <  2:9827576/94‑1 (MQ=255)
                                                                 ccTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGCAt                            >  1:10015205/1‑94 (MQ=255)
                                                                  cTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATc                           <  2:1137940/94‑1 (MQ=255)
                                                                   ttGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCAGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCt                          <  1:7081524/94‑1 (MQ=255)
                                                                          ttCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGt                   >  1:7391451/1‑94 (MQ=255)
                                                                          ttCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGt                   <  1:10592155/94‑1 (MQ=255)
                                                                            cAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTaa                 >  1:6093845/1‑94 (MQ=255)
                                                                            cAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTaa                 >  1:330785/1‑94 (MQ=255)
                                                                             aaCCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAAc                >  1:5899010/1‑94 (MQ=255)
                                                                             aaCCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAAc                <  1:2304880/94‑1 (MQ=255)
                                                                             aaCCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAAc                >  1:6675603/1‑94 (MQ=255)
                                                                             aaCCACGCTTCCCCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAAc                <  1:2058384/94‑1 (MQ=255)
                                                                              aCCACGCTCCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACg               <  1:1953714/94‑1 (MQ=255)
                                                                               ccACGCTTCCGCCGCGACAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGCAATGACCGGTATCTTTGCCGTAACGc              >  1:9885854/1‑94 (MQ=255)
                                                                                 aCGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTc            >  2:2627794/1‑94 (MQ=255)
                                                                                 aCGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTc            >  1:46292/1‑94 (MQ=255)
                                                                                  cGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTcg           >  2:5224869/1‑94 (MQ=255)
                                                                                   cctcccgccgCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTcgc          <  1:2352088/90‑1 (MQ=255)
                                                                                    cTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTcgcg         >  2:1340619/1‑94 (MQ=255)
                                                                                       ccgccgCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCGGTg      <  2:2461183/94‑1 (MQ=255)
                                                                                       ccgccgCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCGGTg      <  1:9174943/94‑1 (MQ=255)
                                                                                           |                                                                                             
CTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCTGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCGGTGCAAG  >  NC_000913/3127774‑3127958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 242 total aligned reads displayed.
GTACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTCA  >  NC_000913/3130241‑3130427
                                                                                             |                                                                                             
GTACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACG                                                                                               <  SRR4302545.10103022/94‑1 (MQ=60)
GTACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACG                                                                                               >  SRR4302545.8534835/1‑94 (MQ=60)
 TACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTGCCGGCACACGAGAGCTCCAACAGACCCAGCAAGGCGCTCACGA                                                                                              <  SRR4302545.7470783/94‑1 (MQ=60)
 TACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGA                                                                                              <  SRR4302545.1520049/94‑1 (MQ=60)
  ACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGAC                                                                                             >  SRR4302545.1296430/1‑94 (MQ=60)
  ACAATTCCACAATCCCCAGTACCCAAAACAGGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGAC                                                                                             <  SRR4302545.7716856/94‑1 (MQ=60)
   CGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACA                                                                                            <  SRR4302545.8899559/94‑1 (MQ=60)
    GATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAACGCGCTCACGACAT                                                                                           >  SRR4302545.6266064/1‑94 (MQ=60)
    GATTGCACAATCACCAGTACGCCAAACATGGCGGGCCGCATCCCTTTACCGGCACACGAAAGCGCCAACAGACCCAGCAAGGCGCCCACGACAT                                                                                           >  SRR4302545.9156598/1‑94 (MQ=60)
    GATCGCAAAAGCACCAGTACGCCAAACCTGGCGGGCTTCATCCCTTTACCGCAACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACAT                                                                                           <  SRR4302545.1143744/94‑1 (MQ=60)
      TTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTA                                                                                         <  SRR4302545.9311489/94‑1 (MQ=60)
      TTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTA                                                                                         <  SRR4302545.4644969/94‑1 (MQ=60)
      TTGCACAATCACCAGTACGCCAAACATGGCGGGCGCCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTA                                                                                         <  SRR4302545.3555910/94‑1 (MQ=60)
        GCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACT                                                                                       >  SRR4302545.2186426/1‑94 (MQ=60)
        GCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACT                                                                                       <  SRR4302545.9067235/94‑1 (MQ=60)
        GCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAACCAGCAAGGCGCTCACGACATTACT                                                                                       >  SRR4302545.3410400/1‑94 (MQ=60)
         CACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTAACGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTG                                                                                      >  SRR4302545.10707975/1‑94 (MQ=60)
         CACAACCACCAGTACGCCAAACGGGGCGGGCGTCATCCCTTTCCCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTG                                                                                      <  SRR4302545.4778844/94‑1 (MQ=60)
          ACAATCACGAGTACGCAAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGG                                                                                     <  SRR4302545.6684431/94‑1 (MQ=60)
          ACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGG                                                                                     >  SRR4302545.10329001/1‑94 (MQ=60)
          ACAACCACCAGTACGCCAACCATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAGGGCGCTCACGACATTACTGG                                                                                     <  SRR4302545.5061548/94‑1 (MQ=60)
             ATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCCACAGACCCCGCAAGGCGCTCACGACCATACTGGAGC                                                                                  >  SRR4302545.6916544/1‑94 (MQ=60)
             ATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGC                                                                                  >  SRR4302545.7409973/1‑94 (MQ=60)
              TCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAAGCGCTCACGACATTACTGGAGCC                                                                                 >  SRR4302545.3272542/1‑94 (MQ=60)
               CACCAGTACGCCAAACATGGCGGGGGTCATCCCCTTACCGGCACACGAGAGGGCCAACAGACACAGCAAGGCGCCCAAGACATTACTGGCGCCT                                                                                >  SRR4302545.3686030/1‑94 (MQ=60)
               CACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCT                                                                                <  SRR4302545.1035594/94‑1 (MQ=60)
               CACCAGTACGCAAACCAGGGCGGGCGTCCTCCCTTTCCCGGCACACGAGAGCGCCAACAGCCCCAGCAGGGCGCTCACGACATTACTGGAGCCT                                                                                <  SRR4302545.4217548/94‑1 (MQ=60)
                 CCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAA                                                                              >  SRR4302545.4204139/1‑94 (MQ=60)
                  CAGTACGCCAACCATGCGGGGCGTCATCCTTGTACCGGCACACGAGAGCGCAACCAGCCCCAGGAAGGCGCCCACGACATTACTGGAGCCTAAC                                                                             <  SRR4302545.4594461/94‑1 (MQ=32)
                  CAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAAC                                                                             <  SRR4302545.1645495/94‑1 (MQ=60)
                    GTCCGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCA                                                                           <  SRR4302545.2871809/94‑1 (MQ=60)
                    GTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCA                                                                           <  SRR4302545.10897255/94‑1 (MQ=60)
                    GTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAAACA                                                                           >  SRR4302545.9897212/1‑94 (MQ=60)
                     TACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAG                                                                          <  SRR4302545.3325044/94‑1 (MQ=60)
                      ccgcaAAACAGGGCGGGCGCCATCCCTTTCCCGCCACACGAGAGCCCCAACAGCCCCACAAGGGCGCCCACGACATTACTGGAGCCTAACCAGG                                                                         <  SRR4302545.9923527/89‑1 (MQ=24)
                      CCGCCAAACATGGCGGGCTTCATCCCTTTACGGGCACACGAGAGCCCCAACAGCCCAACCAAGGCGCTCACGACATTACTGGAGCCTAACCAGG                                                                         <  SRR4302545.6561860/94‑1 (MQ=60)
                      ACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGG                                                                         <  SRR4302545.5720835/94‑1 (MQ=60)
                      ACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGG                                                                         <  SRR4302545.7401080/94‑1 (MQ=60)
                      ACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCCAACCAGG                                                                         >  SRR4302545.9827492/1‑94 (MQ=60)
                       ccaaaaacggggggggcgtcacccctcccCCGGCACAGGAGAGCCCCAACAGCCCCAGCGGGGCGCTCACGACATTACTGGAGCCTAACCAGGC                                                                        <  SRR4302545.240471/65‑1 (MQ=34)
                       CGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGC                                                                        >  SRR4302545.4756820/1‑94 (MQ=60)
                        GCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCG                                                                       <  SRR4302545.10902132/94‑1 (MQ=60)
                        GCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAATGCGCTCACGACATTACTGGAGCCTAACCAGGCG                                                                       <  SRR4302545.5248156/94‑1 (MQ=60)
                        GCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACCTTACTGGAGGCTAACCAGGCG                                                                       >  SRR4302545.5021007/1‑94 (MQ=60)
                        GCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCG                                                                       >  SRR4302545.9414283/1‑94 (MQ=60)
                        GCAAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCG                                                                       <  SRR4302545.950835/94‑1 (MQ=60)
                          caagcggggggggccccccccctctcccgccacaggagggccccaacaccCCCAGCGAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCT                                                                     <  SRR4302545.7911031/44‑1 (MQ=60)
                          aaaaaATGGCGGGCGTCACCCCTTTACCGGCACACGAGAGCGCAAACAGACCCAGCAAGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCT                                                                     <  SRR4302545.4581902/89‑1 (MQ=60)
                          CAACCGTGGCGGGCGTCATCCTTTCCCCGGCACACGAGAGCCCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCT                                                                     <  SRR4302545.8156351/94‑1 (MQ=60)
                          CAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTTTCGACATTACTGTAGCCTAACCAGGCTCT                                                                     >  SRR4302545.9442205/1‑94 (MQ=60)
                          CAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCT                                                                     <  SRR4302545.1337506/94‑1 (MQ=60)
                           AAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTA                                                                    >  SRR4302545.4384186/1‑94 (MQ=60)
                           AAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTA                                                                    >  SRR4302545.4384185/1‑94 (MQ=60)
                           AAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTA                                                                    >  SRR4302545.345990/1‑94 (MQ=60)
                           AAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAAGCGCTCACGACATTAATGGAGCCCAACCAAGCGCTA                                                                    >  SRR4302545.9722112/1‑94 (MQ=60)
                            AACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTAT                                                                   >  SRR4302545.7993078/1‑94 (MQ=60)
                            AACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTAT                                                                   <  SRR4302545.10513105/94‑1 (MQ=60)
                             ACATGGGGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATT                                                                  <  SRR4302545.9227567/94‑1 (MQ=60)
                             ACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATT                                                                  <  SRR4302545.9262714/94‑1 (MQ=60)
                             ACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATT                                                                  >  SRR4302545.7548716/1‑94 (MQ=60)
                             ACAGGGGGGGCGTCATCCCTTTCCGGGCACACGAGAGCGCCAACAGCCCAAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATT                                                                  <  SRR4302545.662607/94‑1 (MQ=60)
                               ATGGCGGTCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGC                                                                <  SRR4302545.5781525/94‑1 (MQ=60)
                               ATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTTC                                                                >  SRR4302545.6536178/1‑94 (MQ=60)
                               ATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGC                                                                >  SRR4302545.6766681/1‑94 (MQ=60)
                               ATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCGATTGC                                                                >  SRR4302545.8814226/1‑94 (MQ=60)
                               ATGGCGGGCGTCATCCCCTTAACGGCACACCAGCGGGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGACTAACCCGGAGCTATTGC                                                                >  SRR4302545.4845246/1‑94 (MQ=53)
                                ggcgggacgtcaccccgctgccggccgacgagagagcgaagagcaccagaacGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCG                                                               <  SRR4302545.4460839/42‑1 (MQ=60)
                                TGGCGGGCGTCACCCCTTTACCGGCACAGGAGAGCGCAAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCG                                                               <  SRR4302545.4460841/94‑1 (MQ=60)
                                 GGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGA                                                              >  SRR4302545.1869526/1‑94 (MQ=60)
                                 GGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGA                                                              >  SRR4302545.1869524/1‑94 (MQ=60)
                                  GCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAA                                                             >  SRR4302545.10420933/1‑94 (MQ=60)
                                   CGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAAT                                                            <  SRR4302545.802339/94‑1 (MQ=60)
                                    gggcctacTCCCTTTCCCGGCAAACGAGAGCGCAAACAGCCCCAGCAAGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATA                                                           <  SRR4302545.8641752/86‑1 (MQ=60)
                                    GGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATA                                                           >  SRR4302545.9272822/1‑94 (MQ=60)
                                     GGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAA                                                          >  SRR4302545.10849440/1‑94 (MQ=60)
                                     GGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAA                                                          >  SRR4302545.6093245/1‑94 (MQ=60)
                                      gcttactcctttccccggcacaggagcgcccaaagagccccagcaggcCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAAT                                                         <  SRR4302545.2682168/46‑1 (MQ=60)
                                      GCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAAT                                                         >  SRR4302545.8055428/1‑94 (MQ=60)
                                       CTTCATCCTTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATG                                                        <  SRR4302545.891833/94‑1 (MQ=60)
                                       CGTCCTCCCTTTACCGGCACACGAGAGCGCCAACAGCCCCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATG                                                        <  SRR4302545.2682852/94‑1 (MQ=60)
                                       CGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATG                                                        >  SRR4302545.1000049/1‑94 (MQ=60)
                                       CGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATG                                                        <  SRR4302545.7632806/94‑1 (MQ=60)
                                       CGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATG                                                        >  SRR4302545.2313259/1‑94 (MQ=60)
                                       CGTCACCCCTTTCCCGGCCCACGAGAGCGCCACCACCCCCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATG                                                        <  SRR4302545.671843/94‑1 (MQ=60)
                                        GTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGG                                                       <  SRR4302545.10211631/94‑1 (MQ=60)
                                        GTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGG                                                       >  SRR4302545.4981870/1‑94 (MQ=60)
                                        GTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGG                                                       <  SRR4302545.10714090/94‑1 (MQ=60)
                                        GTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGG                                                       >  SRR4302545.6434539/1‑94 (MQ=60)
                                        GTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGG                                                       <  SRR4302545.9328825/94‑1 (MQ=60)
                                         TCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGT                                                      <  SRR4302545.5517002/94‑1 (MQ=60)
                                         TCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGT                                                      <  SRR4302545.5324414/94‑1 (MQ=60)
                                         TCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGT                                                      >  SRR4302545.9615729/1‑94 (MQ=60)
                                          cccctcttccccgccacgcgagagccacaacagcccgagcagggccCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTG                                                     <  SRR4302545.2053008/48‑1 (MQ=60)
                                          cacccgtttgccggcacacgaggcccccaacaccCCCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTG                                                     <  SRR4302545.2487969/60‑1 (MQ=60)
                                          cacccctgtcCCGGCACACGAGACCGCCAACAGCCCCAGCAAGCCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTG                                                     <  SRR4302545.332173/84‑1 (MQ=60)
                                          CATCCCTTTCCCGGCACACGAGAGCGCAAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTG                                                     <  SRR4302545.459409/94‑1 (MQ=60)
                                          CATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTG                                                     <  SRR4302545.10794644/94‑1 (MQ=60)
                                          CATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTG                                                     <  SRR4302545.10354051/94‑1 (MQ=60)
                                           ATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGG                                                    >  SRR4302545.7719478/1‑94 (MQ=60)
                                           ATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGC                                                    >  SRR4302545.122481/1‑94 (MQ=60)
                                           ATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGC                                                    >  SRR4302545.3534835/1‑94 (MQ=60)
                                            TCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCG                                                   <  SRR4302545.1807854/94‑1 (MQ=60)
                                             CCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGA                                                  >  SRR4302545.3435685/1‑94 (MQ=60)
                                             CCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGA                                                  >  SRR4302545.3193429/1‑94 (MQ=60)
                                             CCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGA                                                  >  SRR4302545.9566941/1‑94 (MQ=60)
                                              CCTTTCCCGGCACACGAGAGCCCCAACAGCCCCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAA                                                 <  SRR4302545.2394195/94‑1 (MQ=60)
                                              CCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAA                                                 >  SRR4302545.5267022/1‑94 (MQ=60)
                                               cttaacgggcacgcgggggcacaagcggccccagcaggcccCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAA                                                <  SRR4302545.2853301/53‑1 (MQ=60)
                                                TTTACCGGCACACGAGAGCGCCAACAGACCCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAC                                               <  SRR4302545.791058/94‑1 (MQ=60)
                                                TTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAC                                               >  SRR4302545.5883950/1‑94 (MQ=60)
                                                TTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAC                                               <  SRR4302545.8977318/94‑1 (MQ=60)
                                                TTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAC                                               <  SRR4302545.977498/94‑1 (MQ=60)
                                                 TTACCGGCACACGAGAGCGCCAACAGACCCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACG                                              <  SRR4302545.691803/94‑1 (MQ=60)
                                                 TTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACG                                              >  SRR4302545.2586856/1‑94 (MQ=60)
                                                  TACCGGCACACGAGAGCGCCAACAGACTCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGC                                             <  SRR4302545.1322170/94‑1 (MQ=60)
                                                   ACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAGTAATGGTGCGAAACGCG                                            >  SRR4302545.43037/1‑94 (MQ=60)
                                                   ACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCG                                            <  SRR4302545.1911845/94‑1 (MQ=60)
                                                   ACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCG                                            <  SRR4302545.7607978/94‑1 (MQ=60)
                                                   ACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCG                                            <  SRR4302545.1911844/94‑1 (MQ=60)
                                                    CCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGC                                           >  SRR4302545.6006517/1‑94 (MQ=60)
                                                     cgccacagaagagccccaccagcCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCC                                          <  SRR4302545.622130/71‑1 (MQ=60)
                                                       GCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCAT                                        <  SRR4302545.1352544/94‑1 (MQ=60)
                                                        CACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATC                                       >  SRR4302545.9926814/1‑94 (MQ=60)
                                                        CACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATC                                       >  SRR4302545.10082765/1‑94 (MQ=60)
                                                         ACACGAGAGCCCAAACAGCCCAAGCAGGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCT                                      <  SRR4302545.2265618/94‑1 (MQ=60)
                                                          CAGGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTG                                     <  SRR4302545.419882/94‑1 (MQ=60)
                                                          CACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGCGCGAAACGCGCCATCTG                                     <  SRR4302545.8707060/94‑1 (MQ=60)
                                                          CACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTG                                     <  SRR4302545.3369665/94‑1 (MQ=60)
                                                           AGGAGAGCCCCAAAAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGC                                    <  SRR4302545.8595403/94‑1 (MQ=60)
                                                           ACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGC                                    >  SRR4302545.7529885/1‑94 (MQ=60)
                                                           ACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGC                                    >  SRR4302545.1214963/1‑94 (MQ=60)
                                                           ACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGC                                    >  SRR4302545.1648631/1‑94 (MQ=60)
                                                            CGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCA                                   >  SRR4302545.4900414/1‑94 (MQ=60)
                                                             GAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAA                                  <  SRR4302545.5109146/94‑1 (MQ=60)
                                                             GAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAA                                  <  SRR4302545.5109145/94‑1 (MQ=60)
                                                             GAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAA                                  >  SRR4302545.158028/1‑94 (MQ=60)
                                                             GAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAA                                  >  SRR4302545.10804506/1‑94 (MQ=60)
                                                              AGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAA                                 <  SRR4302545.3782994/94‑1 (MQ=60)
                                                              AGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAA                                 >  SRR4302545.3281378/1‑94 (MQ=60)
                                                                agccacaacacgcccagaatgcCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACC                               <  SRR4302545.2250110/72‑1 (MQ=60)
                                                                AGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACC                               >  SRR4302545.5000364/1‑94 (MQ=60)
                                                                 GCGCCAAAAGCCCCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCA                              <  SRR4302545.4836727/94‑1 (MQ=60)
                                                                  CGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAA                             <  SRR4302545.6955113/94‑1 (MQ=60)
                                                                  CGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAA                             <  SRR4302545.6955111/94‑1 (MQ=60)
                                                                  CGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAA                             >  SRR4302545.10865426/1‑94 (MQ=60)
                                                                  CGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAA                             <  SRR4302545.4831161/94‑1 (MQ=60)
                                                                    CCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATG                           <  SRR4302545.4787962/94‑1 (MQ=60)
                                                                    CCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATG                           <  SRR4302545.426659/94‑1 (MQ=60)
                                                                    CCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATG                           <  SRR4302545.5429035/94‑1 (MQ=60)
                                                                    CCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATG                           >  SRR4302545.2577409/1‑94 (MQ=60)
                                                                     CAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGT                          <  SRR4302545.10680460/94‑1 (MQ=60)
                                                                     CAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGT                          <  SRR4302545.10680461/94‑1 (MQ=60)
                                                                     CAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGT                          <  SRR4302545.9393553/94‑1 (MQ=60)
                                                                      cacaccCCCAGCAAGGCCCCCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTT                         <  SRR4302545.2198058/88‑1 (MQ=60)
                                                                      AACAGCCCCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTT                         <  SRR4302545.6034584/94‑1 (MQ=60)
                                                                      AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTT                         <  SRR4302545.8765196/94‑1 (MQ=60)
                                                                      AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTT                         <  SRR4302545.9178875/94‑1 (MQ=60)
                                                                      AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTT                         >  SRR4302545.249743/1‑94 (MQ=60)
                                                                      AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTT                         <  SRR4302545.3058120/94‑1 (MQ=60)
                                                                      AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTT                         >  SRR4302545.2180067/1‑94 (MQ=60)
                                                                       ACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTT                        <  SRR4302545.4753406/94‑1 (MQ=60)
                                                                       ACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTT                        <  SRR4302545.6363142/94‑1 (MQ=60)
                                                                       ACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTT                        <  SRR4302545.914162/94‑1 (MQ=60)
                                                                        CAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTG                       >  SRR4302545.10616594/1‑94 (MQ=60)
                                                                        CAGACCCAGAAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTG                       <  SRR4302545.4862089/94‑1 (MQ=60)
                                                                         AGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGA                      <  SRR4302545.3840886/94‑1 (MQ=60)
                                                                          GCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGAT                     <  SRR4302545.4860937/94‑1 (MQ=60)
                                                                          GACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGAT                     <  SRR4302545.7265157/94‑1 (MQ=60)
                                                                          GACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGAT                     <  SRR4302545.8815418/94‑1 (MQ=60)
                                                                            CCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGT                   >  SRR4302545.6386762/1‑94 (MQ=60)
                                                                            CCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGT                   >  SRR4302545.6386764/1‑94 (MQ=60)
                                                                            CCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGT                   >  SRR4302545.2955806/1‑94 (MQ=60)
                                                                             CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTT                  <  SRR4302545.9378124/94‑1 (MQ=60)
                                                                             CCAGCAAGGCGCCCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTT                  <  SRR4302545.2803459/94‑1 (MQ=60)
                                                                              CAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTA                 >  SRR4302545.5709865/1‑94 (MQ=60)
                                                                               AGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAA                <  SRR4302545.1565597/94‑1 (MQ=60)
                                                                               AGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAA                >  SRR4302545.2555880/1‑94 (MQ=60)
                                                                                GCAGGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAA               <  SRR4302545.261079/94‑1 (MQ=60)
                                                                                GAAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAA               <  SRR4302545.4625691/94‑1 (MQ=60)
                                                                                 CAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAA              >  SRR4302545.2810470/1‑94 (MQ=60)
                                                                                  AAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAC             >  SRR4302545.10858467/1‑94 (MQ=60)
                                                                                    GGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCC           <  SRR4302545.7033402/94‑1 (MQ=60)
                                                                                     GCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCG          <  SRR4302545.7499687/94‑1 (MQ=60)
                                                                                     GCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCG          <  SRR4302545.9170457/94‑1 (MQ=60)
                                                                                     GCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCG          <  SRR4302545.5233402/94‑1 (MQ=60)
                                                                                      CGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCACCTGCAAACCAAcggttgagggtaatacccgc         >  SRR4302545.9633050/1‑74 (MQ=60)
                                                                                       GCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCC        >  SRR4302545.8581453/1‑94 (MQ=60)
                                                                                       GCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCC        >  SRR4302545.8581452/1‑94 (MQ=60)
                                                                                        CTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCA       >  SRR4302545.5653289/1‑94 (MQ=60)
                                                                                        CTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCA       <  SRR4302545.1014278/94‑1 (MQ=60)
                                                                                        CTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCA       >  SRR4302545.863530/1‑94 (MQ=60)
                                                                                        CTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGAGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCA       >  SRR4302545.3146647/1‑94 (MQ=60)
                                                                                        CCCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCA       <  SRR4302545.9530075/94‑1 (MQ=60)
                                                                                         TCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAA      >  SRR4302545.2903670/1‑94 (MQ=60)
                                                                                         TCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAA      <  SRR4302545.5395912/94‑1 (MQ=60)
                                                                                          CACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAAT     >  SRR4302545.7447892/1‑94 (MQ=60)
                                                                                          CACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAAT     >  SRR4302545.4459520/1‑94 (MQ=60)
                                                                                           ACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATT    <  SRR4302545.3912582/94‑1 (MQ=60)
                                                                                             GACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTCA  <  SRR4302545.3184393/94‑1 (MQ=60)
                                                                                             GACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCAAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTCA  <  SRR4302545.3184394/94‑1 (MQ=60)
                                                                                             |                                                                                             
GTACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTCA  >  NC_000913/3130241‑3130427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑