Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,130,334	A→G	V30V (GTT→GTC)	b2983 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,130,334	0	A	G	100.0%	752.6 / NA	214	V30V (GTT→GTC)	b2983	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (90/124); total (90/124)

Only 100 of 237 total aligned reads displayed.

CGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAA  >  NC_000913/3130244‑3130423

cGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACACAACAAAGCGCCCacgac                                                                                         >  2:350221/1‑93 (MQ=255)

  aTTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCCCGACAt                                                                                       >  1:5926914/1‑93 (MQ=255)

   ttGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTCCCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACAtt                                                                                      <  1:3518802/93‑1 (MQ=255)

   ttGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACAtt                                                                                      >  2:741703/1‑93 (MQ=255)

   ttGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAACCAGCAAGGCGCTCACCACCtt                                                                                      >  2:1894060/1‑93 (MQ=255)

   ttGCACAACCACCAGTCCGCAAAACATGGCGGGCGTCATCCCTTTCCCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACAtt                                                                                      <  2:4877166/93‑1 (MQ=255)

      cacaATCACCAGTACGCCAAACATGGCGGGCGTCCTCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAAGCGCTCACGACATTACt                                                                                   >  2:2786061/1‑93 (MQ=255)

       acacTCACAAGTCCGCAAAAGTTGCGGGGCGTCATCCCTTACCCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTg                                                                                  <  2:2289869/93‑1 (MQ=255)

       acaATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTg                                                                                  <  2:6446239/93‑1 (MQ=255)

       acaATCACCAGTACGCAAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTg                                                                                  <  2:6027880/93‑1 (MQ=255)

         aaTCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGa                                                                                >  1:5862668/1‑93 (MQ=255)

         aaTAACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAGGGCGCTCACGACATTACTGGa                                                                                <  2:2039919/93‑1 (MQ=255)

          aTCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAg                                                                               <  2:1841276/93‑1 (MQ=255)

           tCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCCCACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGc                                                                              <  2:1684071/93‑1 (MQ=255)

            cACCAGTACGCAAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGcc                                                                             <  2:2568159/93‑1 (MQ=255)

              ccAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTa                                                                           >  1:1140257/1‑93 (MQ=255)

               cAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTaa                                                                          <  1:4369417/93‑1 (MQ=255)

                aGTACGCCAAACATGGCGGGCGTCATCCCTTTCCCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAAc                                                                         <  2:4161078/93‑1 (MQ=255)

                 gTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGGACACGAGAGCGGCAACAAACGCAGCAAAGGGCTCACGACATTACTTGAGCCTaaac                                                                        >  2:6104687/1‑91 (MQ=255)

                 gTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAAcc                                                                        <  2:5113832/93‑1 (MQ=255)

                  tACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCa                                                                       >  1:3246967/1‑93 (MQ=255)

                  tACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGAGCTCACGACATTACTGGAGCCTCACCa                                                                       >  1:4522500/1‑93 (MQ=255)

                    cGCAAAACATGGCGGGCGTCATCCCTTTCCCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAgg                                                                     <  2:4700747/93‑1 (MQ=255)

                      ccAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTTGAGCCTAACCAGgcg                                                                   >  2:4509396/1‑93 (MQ=255)

                      ccAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGgcg                                                                   >  1:3833560/1‑93 (MQ=255)

                       cAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGgcgc                                                                  >  1:5497994/1‑93 (MQ=255)

                         aaCATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTa                                                                >  1:893592/1‑93 (MQ=255)

                         aaCATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAAGGGCTCACGACATTACTGGAGCCTAAACAGGCGCTa                                                                >  2:4108117/1‑93 (MQ=255)

                           cagggcgggcgTCCTCCCTTTCCCGCCACACGAGAGCACCACCAGCCCCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTAtt                                                              <  2:3576867/90‑1 (MQ=255)

                           cATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTAtt                                                              >  2:3015241/1‑93 (MQ=255)

                             tGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGc                                                            <  2:5031369/93‑1 (MQ=255)

                              ggcgggcgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCg                                                           >  2:6393470/1‑93 (MQ=255)

                                ggggcgTCATCCCTTTACCGGCAGACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaa                                                         <  2:857213/92‑1 (MQ=255)

                                cgggcgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaa                                                         <  1:6436654/93‑1 (MQ=255)

                                cgggcgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaa                                                         <  2:6209230/93‑1 (MQ=255)

                                  ggcgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaata                                                       >  1:1074697/1‑93 (MQ=255)

                                   gcgTCATCCCTTTCCCGGCACGCGAGAGCGCCAACAGACCCAGAAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaataa                                                      <  2:5842739/93‑1 (MQ=255)

                                   gcgTCATCCCTTTCCCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaataa                                                      <  2:2904127/93‑1 (MQ=255)

                                    cgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaataat                                                     <  2:5165062/93‑1 (MQ=255)

                                    cgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaataat                                                     <  2:1728161/93‑1 (MQ=255)

                                      tcgtCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATgg                                                   <  1:1926385/90‑1 (MQ=255)

                                       cATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGt                                                  >  2:3395236/1‑93 (MQ=255)

                                       cATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGt                                                  <  2:3118561/93‑1 (MQ=255)

                                       cATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGt                                                  <  2:711793/93‑1 (MQ=255)

                                       cATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGt                                                  >  2:5282922/1‑93 (MQ=255)

                                          cccTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGACTAACCAGGCGCTATTGCGAATAATGGTGCg                                               >  1:4159991/1‑93 (MQ=255)

                                           ccTTTACCGGCACACCAGCGCCCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGa                                              <  1:1885125/93‑1 (MQ=255)

                                            cTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGaa                                             >  1:801481/1‑93 (MQ=255)

                                            cTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGaa                                             >  2:3675131/1‑93 (MQ=255)

                                             tttACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGGGCTATTGCGAATAATGTGGCGaaa                                            >  1:3276625/1‑93 (MQ=255)

                                             tttACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGaaa                                            >  1:2683299/1‑93 (MQ=255)

                                             tttACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGaaa                                            <  2:5389499/93‑1 (MQ=255)

                                             tttACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGaaa                                            <  1:5402045/93‑1 (MQ=255)

                                             tttACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGaaa                                            >  1:4221268/1‑93 (MQ=255)

                                              ttACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAc                                           <  1:6400977/93‑1 (MQ=255)

                                              ttACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAc                                           <  1:4741700/93‑1 (MQ=255)

                                              tgaCCGGCACACGAGAGCGCAAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAc                                           <  2:2375965/91‑1 (MQ=255)

                                              tcaCCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAc                                           >  2:5134489/3‑93 (MQ=255)

                                               tACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAcg                                          >  1:944754/1‑93 (MQ=255)

                                                 ccGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAcgcg                                        <  1:4085777/93‑1 (MQ=255)

                                                  cGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACgcgc                                       >  1:1814696/1‑93 (MQ=255)

                                                    caacacGGGAGCCCAAACAGACCCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCa                                     <  2:153310/91‑1 (MQ=255)

                                                      acacGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATc                                   <  2:3920959/93‑1 (MQ=255)

                                                       cacGAGAGCGCCAACAGACCCAGCAGGCCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCt                                  <  1:231491/93‑1 (MQ=255)

                                                       cacGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCt                                  >  1:2248690/1‑93 (MQ=255)

                                                         cGAGAGCGCCAACAAACCCAGCAAGGCGCCCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAACCGCGCCATCTGc                                <  2:996235/93‑1 (MQ=255)

                                                          gagaGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCa                               >  1:5074850/1‑93 (MQ=255)

                                                             agCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAAc                            <  2:1429096/93‑1 (MQ=255)

                                                                cccAACAGCCCCGGCAGGGCCCCCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCaa                         <  2:5424816/92‑1 (MQ=255)

                                                                 ccAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAAt                        >  1:3030508/1‑93 (MQ=255)

                                                                 ccAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAAt                        <  2:2975929/93‑1 (MQ=255)

                                                                   aaCAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGt                      >  1:5187910/1‑93 (MQ=255)

                                                                   aaCAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGt                      >  2:2549587/1‑93 (MQ=255)

                                                                   aaCAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGt                      <  1:2898244/93‑1 (MQ=255)

                                                                     cAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGttt                    <  2:6085313/93‑1 (MQ=255)

                                                                      aGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTg                   >  1:3373926/1‑93 (MQ=255)

                                                                       gccccAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGa                  <  1:2757655/91‑1 (MQ=255)

                                                                       gACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGa                  <  2:5319288/93‑1 (MQ=255)

                                                                       gACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGa                  >  2:1134761/1‑93 (MQ=255)

                                                                       gACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGa                  >  2:3418259/1‑93 (MQ=255)

                                                                        aCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGAt                 <  1:2831721/93‑1 (MQ=255)

                                                                        aCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGAt                 <  2:4015806/93‑1 (MQ=255)

                                                                          ccAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGt               <  2:4274973/93‑1 (MQ=255)

                                                                          ccAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGt               <  2:3185849/93‑1 (MQ=255)

                                                                           cAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGCTTGATGta              >  1:3919844/1‑92 (MQ=255)

                                                                             gCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTaa            <  2:5194737/93‑1 (MQ=255)

                                                                             gCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTaa            >  1:4430768/1‑93 (MQ=255)

                                                                             gCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTaa            >  1:1467174/1‑93 (MQ=255)

                                                                             gCAAGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTaa            <  1:2393866/93‑1 (MQ=255)

                                                                              cAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTaaa           <  2:2211624/93‑1 (MQ=255)

                                                                               aaGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTaaaa          >  2:2510891/1‑93 (MQ=255)

                                                                                aGGCGCTCACGACATTGCTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAc         <  2:6286123/93‑1 (MQ=255)

                                                                                aGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAc         >  1:3876222/1‑93 (MQ=255)

                                                                                aGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAc         >  2:3760417/1‑93 (MQ=255)

                                                                                 ggCTCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAcc        <  1:2558387/93‑1 (MQ=255)

                                                                                 ggCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAcc        <  2:5688120/93‑1 (MQ=255)

                                                                                    gcTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCACACCAATGTTTGATGTTAAAATCCGc     >  2:718193/1‑93 (MQ=255)

                                                                                      tCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCCATGTTTGATGTTAAAACCCGCCa   >  1:6044357/1‑93 (MQ=255)

                                                                                      tCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCa   >  2:5766097/1‑93 (MQ=255)

                                                                                       cacgACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCaa  <  2:5977145/93‑1 (MQ=255)

CGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAA  >  NC_000913/3130244‑3130423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTCA  >  NC_000913/3130242‑3130427

TACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGA                                                                                              <  SRR4302537.421581/94‑1 (MQ=60)

   GATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCCCGACAT                                                                                           >  SRR4302537.5967590/1‑94 (MQ=60)

   GATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACAT                                                                                           >  SRR4302537.3931660/1‑94 (MQ=60)

   GATTGCACAATCACCAGTACGCCAAACATGGCGGGCCGCATTCCTTTACCCGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCCCGACAT                                                                                           >  SRR4302537.1228392/1‑94 (MQ=60)

     TTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTCCCGGCACACGAGAGCGCCAACAGCC‑‑CCAGCAAGGCGCTCACGACATTA                                                                                         <  SRR4302537.3543701/94‑1 (MQ=60)

     TTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACTGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTA                                                                                         <  SRR4302537.4218111/94‑1 (MQ=60)

     TTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTA                                                                                         >  SRR4302537.2550038/1‑94 (MQ=60)

        CACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTG                                                                                      >  SRR4302537.4337886/1‑94 (MQ=60)

        AACAATCACCAGTACGCTAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTG                                                                                      <  SRR4302537.1934205/94‑1 (MQ=60)

         ACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCACCAGAC‑‑CCCGCAAGGCGCTCACGACCTTACTGC                                                                                     >  SRR4302537.2453516/1‑94 (MQ=60)

         ACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACCGAC‑‑CCAGCAACGCGCTCACGACATTACTTG                                                                                     >  SRR4302537.5443283/1‑94 (MQ=60)

          CAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGA                                                                                    >  SRR4302537.5902874/1‑94 (MQ=60)

           AATCACCAGTACGCCAAACATGGCGGGCGTCATCCCCTTACCGGCACACCAGAGCGCCAACACAC‑‑CCACCAAGGCGCTCACGACATTACTGGAG                                                                                   >  SRR4302537.5465084/1‑94 (MQ=60)

           AACCACCAGTCCGCAAAACATGGCGGGCGTCATCCTTTTACCGGCACACGAGAGCGCCAACAGCC‑‑CCAGCAGGGCGCTCACGACATTACTGGAG                                                                                   <  SRR4302537.1417077/94‑1 (MQ=60)

             TCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAGGGCGCTCACGACATTACTGGAGCC                                                                                 <  SRR4302537.2868157/94‑1 (MQ=60)

               ACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTA                                                                               >  SRR4302537.1148178/1‑94 (MQ=60)

               ACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTA                                                                               <  SRR4302537.3302140/94‑1 (MQ=60)

                CCAGTACGCAAAACATGGCGGGCGTCATCCATAAGCCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAA                                                                              <  SRR4302537.240426/94‑1 (MQ=60)

                 CAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAAC                                                                             <  SRR4302537.4399614/94‑1 (MQ=60)

                  AGTACGCCAAACATGGCGGGCGGCATCCCTTTACCGGCACAAGAGAGCGCCAACAGAC‑‑CCAGCAATGCGCTCACGACATTACTGGAGCCTAAaa                                                                            >  SRR4302537.3920291/1‑92 (MQ=60)

                   gtcctcaAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCAAACAGAC‑‑CCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCA                                                                           <  SRR4302537.2685486/87‑1 (MQ=60)

                   GTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCA                                                                           >  SRR4302537.3269850/1‑94 (MQ=60)

                   GTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGAGCTCACGACATTACTGGAGCCTCACCA                                                                           >  SRR4302537.4553789/1‑94 (MQ=60)

                     ACGCAAAACATGGCGGGCGTCATCCCTTCACCGGCACACGAGAGCGCCAACAGCC‑‑CCAGAAAGGCGCTCACGACATTACTGGAGCCTAACCAGG                                                                         <  SRR4302537.1480550/94‑1 (MQ=60)

                       GCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCG                                                                       <  SRR4302537.6475132/94‑1 (MQ=60)

                       GCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCG                                                                       >  SRR4302537.3860428/1‑94 (MQ=60)

                        CCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGC                                                                      >  SRR4302537.5535529/1‑94 (MQ=60)

                          AAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTA                                                                    >  SRR4302537.899758/1‑94 (MQ=60)

                           AACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTAT                                                                   >  SRR4302537.4417433/1‑94 (MQ=60)

                           AACAGGGCGGGCTTTATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTAT                                                                   <  SRR4302537.2005870/94‑1 (MQ=60)

                             AATGGCGGGCGTCACCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTG                                                                 <  SRR4302537.2826405/94‑1 (MQ=60)

                                  CGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAAT                                                            <  SRR4302537.6480356/94‑1 (MQ=60)

                                   GGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGCC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATA                                                           <  SRR4302537.2760058/94‑1 (MQ=60)

                                   GGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATA                                                           >  SRR4302537.1082097/1‑94 (MQ=60)

                                     GCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAAT                                                         >  SRR4302537.204433/1‑94 (MQ=60)

                                     GCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAAT                                                         <  SRR4302537.704073/94‑1 (MQ=60)

                                      CGTCATCCTTTTCCCGGCACACGAGAGCGCAAACAGCC‑‑CCAGCAAGCCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATG                                                        <  SRR4302537.2464039/94‑1 (MQ=60)

                                       GTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGG                                                       <  SRR4302537.4342358/94‑1 (MQ=60)

                                        TCGTCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGT                                                      <  SRR4302537.1939753/94‑1 (MQ=60)

                                         caccccttcccgGGCACACGAGAGCCCAACCAGGC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTG                                                     <  SRR4302537.4762570/82‑1 (MQ=60)

                                         CATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTG                                                     <  SRR4302537.595471/94‑1 (MQ=60)

                                           TCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCG                                                   >  SRR4302537.5733799/1‑94 (MQ=60)

                                           TCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCG                                                   >  SRR4302537.6025219/1‑94 (MQ=60)

                                           TCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGACTAACCAGGCGCTATTGCGAATAATGGTGCG                                                   >  SRR4302537.4189044/1‑94 (MQ=60)

                                            CCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGA                                                  >  SRR4302537.2743463/1‑94 (MQ=60)

                                             CCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAA                                                 >  SRR4302537.806986/1‑94 (MQ=60)

                                             CCTTTACCGGCACACCAGCGCCCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAA                                                 <  SRR4302537.1898235/94‑1 (MQ=60)

                                              CTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGGGCTATTGCGAATAATGTGGCGAAA                                                >  SRR4302537.3299747/1‑94 (MQ=60)

                                              CTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAA                                                >  SRR4302537.3586896/1‑94 (MQ=60)

                                              CTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAA                                                >  SRR4302537.2702030/1‑94 (MQ=60)

                                              CTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAA                                                >  SRR4302537.4250676/1‑94 (MQ=60)

                                               TTTACCGGCACCCGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAC                                               <  SRR4302537.3864370/94‑1 (MQ=60)

                                               TTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAC                                               <  SRR4302537.5438910/94‑1 (MQ=60)

                                                TTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACG                                              <  SRR4302537.4774470/94‑1 (MQ=60)

                                                TTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACG                                              >  SRR4302537.951283/1‑94 (MQ=60)

                                                TTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACG                                              <  SRR4302537.6444552/94‑1 (MQ=60)

                                                 TCCCGCCACACGAGAGCGCCAACAGCC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGC                                             <  SRR4302537.4629055/94‑1 (MQ=60)

                                                  ACCGGCACACGAGAGCTCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCG                                            <  SRR4302537.4094548/94‑1 (MQ=60)

                                                   CCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGC                                           <  SRR4302537.4114308/94‑1 (MQ=60)

                                                   CCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGC                                           >  SRR4302537.4497545/1‑94 (MQ=60)

                                                   CCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGC                                           >  SRR4302537.1827346/1‑94 (MQ=60)

                                                    CGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCC                                          <  SRR4302537.4773278/94‑1 (MQ=60)

                                                    CGGCACACGAGAGCCCAAACAGACCACCAGC‑‑GGCGCACACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCC                                          <  SRR4302537.2315425/94‑1 (MQ=60)

                                                      GCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCAT                                        >  SRR4302537.3347124/1‑94 (MQ=60)

                                                       CACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATC                                       >  SRR4302537.1875037/1‑94 (MQ=60)

                                                        ACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCT                                      >  SRR4302537.2264561/1‑94 (MQ=60)

                                                        ACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCT                                      <  SRR4302537.818797/94‑1 (MQ=60)

                                                         CACGAGAGCGCCAACAGAC‑‑CCAGCAGGCCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTG                                     <  SRR4302537.233121/94‑1 (MQ=60)

                                                         CACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTG                                     <  SRR4302537.6132728/94‑1 (MQ=60)

                                                         CACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTG                                     <  SRR4302537.292972/94‑1 (MQ=60)

                                                          ACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGACACGCGCCATCTGC                                    >  SRR4302537.3715609/1‑94 (MQ=60)

                                                           CGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCA                                   >  SRR4302537.5109789/1‑94 (MQ=60)

                                                                  GCCAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAAT                            >  SRR4302537.3051638/1‑94 (MQ=60)

                                                                    CAACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGT                          >  SRR4302537.5223590/1‑94 (MQ=60)

                                                                     AACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTT                         >  SRR4302537.5199679/1‑94 (MQ=60)

                                                                     AACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTT                         <  SRR4302537.2918609/94‑1 (MQ=60)

                                                                     AACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTT                         <  SRR4302537.3288028/94‑1 (MQ=60)

                                                                      ACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTT                        <  SRR4302537.4072160/94‑1 (MQ=60)

                                                                      ACAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTT                        <  SRR4302537.2985606/94‑1 (MQ=60)

                                                                       CAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTG                       >  SRR4302537.4819995/1‑94 (MQ=60)

                                                                       CAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTG                       >  SRR4302537.3397835/1‑94 (MQ=60)

                                                                       CAGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTG                       <  SRR4302537.3064710/94‑1 (MQ=60)

                                                                        AGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGA                      <  SRR4302537.5173366/94‑1 (MQ=60)

                                                                        AGAC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGA                      >  SRR4302537.1169295/1‑94 (MQ=60)

                                                                         GCC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGAT                     <  SRR4302537.2776904/94‑1 (MQ=60)

                                                                          AC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATG                    <  SRR4302537.674394/94‑1 (MQ=60)

                                                                          AC‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATG                    <  SRR4302537.2851506/94‑1 (MQ=60)

                                                                           C‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGT                   >  SRR4302537.959500/1‑94 (MQ=60)

                                                                           C‑‑CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGT                   >  SRR4302537.4164202/1‑94 (MQ=60)

                                                                              CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTT                  >  SRR4302537.1502001/1‑94 (MQ=60)

                                                                              CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGCTTGATGTA                  >  SRR4302537.3947240/1‑94 (MQ=60)

                                                                               CAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTA                 <  SRR4302537.5347003/94‑1 (MQ=60)

                                                                                AGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAA                >  SRR4302537.6368422/1‑94 (MQ=60)

                                                                                AGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAA                >  SRR4302537.1477378/1‑94 (MQ=60)

                                                                                AGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAA                >  SRR4302537.4461366/1‑94 (MQ=60)

                                                                                 GCAAGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAA               <  SRR4302537.2410728/94‑1 (MQ=60)

                                                                                  CAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAA              <  SRR4302537.1083653/94‑1 (MQ=60)

                                                                                   AAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCGTCTGCAAACCAATGTTTGATGTTAAAAC             >  SRR4302537.2609016/1‑94 (MQ=60)

                                                                                   AAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAC             >  SRR4302537.3903335/1‑94 (MQ=60)

                                                                                   AAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAC             >  SRR4302537.5885323/1‑94 (MQ=60)

                                                                                     GGCTCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCC           <  SRR4302537.2576318/94‑1 (MQ=60)

                                                                                     GGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTNCGAAACGCGCCNTCNGCNNNNNAATGTTNGATGNTNANANCC           >  SRR4302537.2862812/1‑94 (MQ=60)

                                                                                     GGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCC           <  SRR4302537.3497643/94‑1 (MQ=60)

                                                                                         CTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCCATGTTTGATGTTAAAACCCGCCA       >  SRR4302537.6085872/1‑94 (MQ=60)

                                                                                         CTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCGGCCA       >  SRR4302537.90972/1‑94 (MQ=60)

                                                                                           CACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAAT     >  SRR4302537.3465502/1‑94 (MQ=60)

                                                                                            ACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATT    <  SRR4302537.123875/94‑1 (MQ=60)

                                                                                              GACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTCA  <  SRR4302537.4540575/94‑1 (MQ=60)

                                                                                              GACATTACGGGAGCCTAACCAGGCGCTATTGCGAATAATGGTCCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAACCCCGCCAATTCA  <  SRR4302537.2530927/94‑1 (MQ=60)

TACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAC‑‑CCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTCA  >  NC_000913/3130242‑3130427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`