Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,175,745 A→G V61A (GTC→GCC)  yqiE ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,175,7450AG99.2% 1554.8 / ‑0.7 477V61A (GTC→GCC) yqiEhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base G (259/213);  total (261/216)
Fisher's exact test for biased strand distribution p-value = 3.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.93e-01

Only 100 of 518 total aligned reads displayed.

TAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTA  >  NC_000913/3175654‑3175835
                                                                                           |                                                                                          
 aGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTg                                                                                         >  1:8325250/1‑94 (MQ=255)
  gctaaGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTgc                                                                                        <  1:4895749/91‑1 (MQ=255)
  gCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTgc                                                                                        <  2:8044816/94‑1 (MQ=255)
  gCCAAGGGGGCTCTCGGTGGTCGTACGCGGCAACCCGCATCGGCTCAATCAGCACAACTTCATCACGCACTGGGTAAAAGGGGAGCAAGGCTgc                                                                                        <  2:10285517/94‑1 (MQ=255)
    cAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTgcgg                                                                                      >  2:1613333/1‑94 (MQ=255)
     aaGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTgcggc                                                                                     >  1:1922956/1‑94 (MQ=255)
     aaGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTgcggc                                                                                     <  1:2224908/94‑1 (MQ=255)
      aGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTgcggcg                                                                                    >  1:1092531/1‑94 (MQ=255)
      aGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTgcggcg                                                                                    >  2:1504265/1‑94 (MQ=255)
        gggTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTg                                                                                  >  1:8035294/1‑94 (MQ=255)
           tttCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCACGGCTGCGGAGTGAcc                                                                               >  2:3529001/1‑94 (MQ=255)
             tCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCGATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACcgc                                                                             <  1:4734490/94‑1 (MQ=255)
             tCGCTGGTGTCGTACGCGGCAACCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACcgc                                                                             >  1:2971569/1‑94 (MQ=255)
               gCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCgcgc                                                                           >  1:4583267/1‑94 (MQ=255)
                 tGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTc                                                                         >  2:8238684/1‑94 (MQ=255)
                  ggTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCa                                                                        >  2:8335955/1‑94 (MQ=255)
                     gtCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACTGCGCTCaaaa                                                                     >  1:8871376/1‑94 (MQ=255)
                     gtCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCaaaa                                                                     <  2:6345093/94‑1 (MQ=255)
                      tCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAA‑GGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAAt                                                                   <  2:7254738/94‑1 (MQ=255)
                        gTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAAtt                                                                  >  1:7275986/1‑94 (MQ=255)
                         tACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAAttt                                                                 <  2:5043158/94‑1 (MQ=255)
                          aCGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTc                                                                <  2:6470146/94‑1 (MQ=255)
                            gcgGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTccc                                                              <  2:8812989/94‑1 (MQ=255)
                            gcgGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTccc                                                              >  1:2299877/1‑94 (MQ=255)
                             cgGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAACTTTCacg                                                             >  1:6627870/1‑94 (MQ=255)
                             cgGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGAACGCGCTCAAAAATTTCccg                                                             >  1:1522640/1‑94 (MQ=255)
                              ggCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCccgc                                                            >  2:13549/1‑94 (MQ=255)
                               gCAATCCGAATCTGCTCAATCAGCACACCTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCccgcc                                                           <  1:7199572/94‑1 (MQ=255)
                               gCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCccgcc                                                           >  2:653791/1‑94 (MQ=255)
                                cAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCccgccg                                                          >  2:10574716/1‑94 (MQ=255)
                                cAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCccgccg                                                          >  2:239731/1‑94 (MQ=255)
                                    ccGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCAcc                                                      >  2:7032181/1‑94 (MQ=255)
                                    ccGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGCCAAACGGTAGCAAGGCTGCGGCGGGCGCGCGTTCCAACACTTCCTGCCGCAct                                                      >  2:8665424/1‑93 (MQ=255)
                                     cGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCt                                                     >  1:9569595/1‑94 (MQ=255)
                                     cGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCt                                                     >  1:3134483/1‑94 (MQ=255)
                                     cGAATCTGCTCAATCAGCACAACTTCATCACGCACGGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCt                                                     <  2:540609/94‑1 (MQ=255)
                                      gAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTc                                                    >  1:4315277/1‑94 (MQ=255)
                                       aaTCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCa                                                   >  1:3943134/1‑94 (MQ=255)
                                        aTCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCAt                                                  <  1:8198843/94‑1 (MQ=255)
                                         tCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATg                                                 <  2:393876/94‑1 (MQ=255)
                                         tCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATg                                                 >  2:9906662/1‑94 (MQ=255)
                                           tGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGAc                                               >  2:9656908/1‑94 (MQ=255)
                                             cTCAATCAGCCCAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTc                                             <  1:893833/94‑1 (MQ=255)
                                             cTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTc                                             >  1:3422744/1‑94 (MQ=255)
                                                aaTCAGCACAACTTCCTCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGCCCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCAtt                                          <  2:3777612/94‑1 (MQ=255)
                                                aaTCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCAtt                                          >  1:7803553/1‑94 (MQ=255)
                                                aaTCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCAtt                                          <  1:2660574/94‑1 (MQ=255)
                                                 aTCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGCCCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCAttt                                         <  1:7036779/94‑1 (MQ=255)
                                                 aTCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCAttt                                         <  1:6362581/94‑1 (MQ=255)
                                                  tCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTg                                        <  1:10540749/94‑1 (MQ=255)
                                                    aGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGcc                                      >  2:2067243/1‑94 (MQ=255)
                                                       acaACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGt                                   >  1:9117634/1‑94 (MQ=255)
                                                       acaACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGt                                   >  1:5619928/1‑94 (MQ=255)
                                                        caACTTCATCCCGCCCTGGGTCAAAGGGTAGCAAGGCTGCGGCGGGCCCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGtt                                  <  1:646307/94‑1 (MQ=255)
                                                        caACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGtt                                  >  1:2902748/1‑94 (MQ=255)
                                                        caACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGtt                                  >  1:127831/1‑94 (MQ=255)
                                                        caACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCAGCCGCACCTCATGACTCATTTGCCCGtt                                  >  2:6818363/1‑94 (MQ=255)
                                                         aaCTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTg                                 <  1:9294075/94‑1 (MQ=255)
                                                          aCTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGa                                <  1:10086737/94‑1 (MQ=255)
                                                          aCTCCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGa                                <  1:4112344/94‑1 (MQ=255)
                                                            ttCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGTTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAAt                              <  2:6064064/94‑1 (MQ=255)
                                                            ctcatcaCGCACTGGGTCAAGGGTTAGCAAGTCTGCGGCGTGACCGCCCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAAt                              <  2:6659126/93‑1 (MQ=255)
                                                             tcatcaCGCACTGGGTCAAAGGGTAGCAGGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATa                             <  1:6152155/94‑1 (MQ=255)
                                                             tcatcaCGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATa                             <  1:10235707/94‑1 (MQ=255)
                                                             tcatcaCGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATa                             >  1:8567142/1‑94 (MQ=255)
                                                             tcatcaCGCACGGGGTCAAAGGGTAGCAAGCCTCCGGCGTGACCGCGCTCAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATa                             <  1:6007596/94‑1 (MQ=255)
                                                                 caCGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACg                         >  1:3287976/1‑94 (MQ=255)
                                                                 caCGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACg                         <  1:6749922/94‑1 (MQ=255)
                                                                   cGCAGTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGAt                       <  1:1706020/94‑1 (MQ=255)
                                                                   cGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGAt                       >  2:4471764/1‑94 (MQ=255)
                                                                   cGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGAt                       >  2:3090783/1‑94 (MQ=255)
                                                                   cGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGAt                       >  2:9286764/1‑94 (MQ=255)
                                                                    gCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATg                      >  1:380875/1‑94 (MQ=255)
                                                                    gCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATg                      <  1:24470/94‑1 (MQ=255)
                                                                     cattGGGTCAACGGGTAGAAGGGCTGCGGCGTGACCGCGCTCCAAAATTTCCCGCCGCCCCTCATGACTCATTTGCCCGTTGAATAGACGATGa                     <  2:5693365/91‑1 (MQ=255)
                                                                      aCTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGAc                    <  2:1712753/94‑1 (MQ=255)
                                                                      aCTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGAc                    >  2:5220958/1‑94 (MQ=255)
                                                                      aCTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGAc                    >  2:5327338/1‑94 (MQ=255)
                                                                      aCTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGAc                    >  1:5173579/1‑94 (MQ=255)
                                                                       cTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACg                   <  2:5898932/94‑1 (MQ=255)
                                                                           gTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAAt               >  2:4841084/1‑94 (MQ=255)
                                                                           gTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAAt               <  1:3645154/94‑1 (MQ=255)
                                                                           gTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAAt               >  2:7838434/1‑94 (MQ=255)
                                                                            tCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATc              <  2:2809940/94‑1 (MQ=255)
                                                                            tCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATc              <  2:7631529/94‑1 (MQ=255)
                                                                             cAAAGGGTAGCAGGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCt             <  1:7627733/94‑1 (MQ=255)
                                                                             cAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCt             >  2:7164962/1‑94 (MQ=255)
                                                                             cAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCt             >  2:6907634/1‑94 (MQ=255)
                                                                             cAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCt             <  1:74709/94‑1 (MQ=255)
                                                                              aaaGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCta            >  2:3384240/1‑94 (MQ=255)
                                                                                 gggTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATaa         >  2:9045765/1‑94 (MQ=255)
                                                                                 ggctAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATaa         <  2:3269142/91‑1 (MQ=255)
                                                                                    tAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGa      <  1:9716409/94‑1 (MQ=255)
                                                                                    tAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGa      <  2:10163500/94‑1 (MQ=255)
                                                                                    tAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGa      <  2:1243129/94‑1 (MQ=255)
                                                                                      gCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATc    <  1:9952539/94‑1 (MQ=255)
                                                                                      gCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATc    >  2:4386604/1‑94 (MQ=255)
                                                                                        aaGGCTGCGGCGTGACCGCGCTCAAGAATTTCCTGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGATATCTATAAAGATCTa  >  2:9419686/1‑94 (MQ=255)
                                                                                        aaGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTa  >  1:4469730/1‑94 (MQ=255)
                                                                                        aaGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTa  <  2:4641377/94‑1 (MQ=255)
                                                                                           |                                                                                          
TAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTA  >  NC_000913/3175654‑3175835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 307 total aligned reads displayed.
CCAGTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTG  >  NC_000913/3178949‑3179135
                                                                                             |                                                                                             
CCAGTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGCAGATCGCGGAACCATTCAGTATTTGCAGGACTGAGCAACGGAAGATGAAATTGCC                                                                                               >  SRR4302545.5042060/1‑94 (MQ=60)
CCAGTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCC                                                                                               >  SRR4302545.1202519/1‑94 (MQ=60)
CCAGTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCC                                                                                               <  SRR4302545.7701763/94‑1 (MQ=60)
  AGTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGCAATTGCCAC                                                                                             >  SRR4302545.462090/1‑94 (MQ=60)
   GTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACT                                                                                            <  SRR4302545.9861345/94‑1 (MQ=60)
   GTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATAGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACT                                                                                            >  SRR4302545.6604541/1‑94 (MQ=60)
    TTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTG                                                                                           <  SRR4302545.8381416/94‑1 (MQ=60)
     TGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGA                                                                                          <  SRR4302545.10160302/94‑1 (MQ=60)
     TGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGA                                                                                          <  SRR4302545.4127377/94‑1 (MQ=60)
      TCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGATGAAATTGCCACTGAG                                                                                         <  SRR4302545.3698894/94‑1 (MQ=60)
       CTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGT                                                                                        >  SRR4302545.358553/1‑94 (MQ=60)
        TGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTT                                                                                       >  SRR4302545.423428/1‑94 (MQ=60)
        TGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTT                                                                                       <  SRR4302545.3123962/94‑1 (MQ=60)
        TGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATCTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTT                                                                                       >  SRR4302545.6738321/1‑94 (MQ=60)
           ATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCT                                                                                    >  SRR4302545.4271078/1‑94 (MQ=60)
           ATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCT                                                                                    <  SRR4302545.1543599/94‑1 (MQ=60)
           ATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCT                                                                                    <  SRR4302545.5833516/94‑1 (MQ=60)
           ATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGATCCT                                                                                    >  SRR4302545.9523943/1‑94 (MQ=60)
            TCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTC                                                                                   <  SRR4302545.9087627/94‑1 (MQ=60)
             CTCGCCTGCTGTCGCGACACGGTAGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCT                                                                                  <  SRR4302545.5801969/94‑1 (MQ=60)
             CTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCT                                                                                  <  SRR4302545.10161346/94‑1 (MQ=60)
             CTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCT                                                                                  >  SRR4302545.3408512/1‑94 (MQ=60)
              TCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTA                                                                                 <  SRR4302545.6758181/94‑1 (MQ=60)
              TCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCACTCAGTATTTGCAGGTCTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTA                                                                                 <  SRR4302545.6360903/94‑1 (MQ=60)
               CACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTAC                                                                                >  SRR4302545.5863471/1‑94 (MQ=60)
               CACCTGCTGTCGCGACACGGTGGAAGATCGC‑GAACCCTTCAGTATTTGCAGGACTGCGCCACGGAAGCTGAAATTGCCACTGAGTTCCTCTACA                                                                               >  SRR4302545.495935/1‑94 (MQ=60)
                ACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACA                                                                               <  SRR4302545.1103156/94‑1 (MQ=60)
                 CCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACAT                                                                              >  SRR4302545.3280940/1‑94 (MQ=60)
                 CCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACAT                                                                              <  SRR4302545.938737/94‑1 (MQ=60)
                 CCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACAT                                                                              <  SRR4302545.1584452/94‑1 (MQ=60)
                 CCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACAT                                                                              >  SRR4302545.6474639/1‑94 (MQ=60)
                 CCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACAT                                                                              <  SRR4302545.7223014/94‑1 (MQ=60)
                 CCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACAT                                                                              >  SRR4302545.9152125/1‑94 (MQ=60)
                 CCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACAT                                                                              <  SRR4302545.2886389/94‑1 (MQ=60)
                  CTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATC                                                                             <  SRR4302545.5384977/94‑1 (MQ=60)
                  CTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATC                                                                             <  SRR4302545.2577753/94‑1 (MQ=60)
                  CTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATC                                                                             >  SRR4302545.3754532/1‑94 (MQ=60)
                   TGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCG                                                                            <  SRR4302545.8512224/94‑1 (MQ=60)
                    GCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGA                                                                           >  SRR4302545.10124592/1‑94 (MQ=60)
                     CTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGAT                                                                          <  SRR4302545.8967307/94‑1 (MQ=60)
                     CTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGAT                                                                          >  SRR4302545.5444902/1‑94 (MQ=60)
                      TGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATG                                                                         >  SRR4302545.3754034/1‑94 (MQ=60)
                      TGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATG                                                                         <  SRR4302545.3341107/94‑1 (MQ=60)
                      TGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATG                                                                         <  SRR4302545.2603320/94‑1 (MQ=60)
                       GTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGA                                                                        <  SRR4302545.9857967/94‑1 (MQ=60)
                        TCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGAT                                                                       <  SRR4302545.9034121/94‑1 (MQ=60)
                        TCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGAT                                                                       <  SRR4302545.8296891/94‑1 (MQ=60)
                         CGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATA                                                                      <  SRR4302545.2382470/94‑1 (MQ=60)
                         CGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATA                                                                      >  SRR4302545.8385199/1‑94 (MQ=60)
                         CGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGAAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATA                                                                      >  SRR4302545.1472667/1‑94 (MQ=60)
                          GCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATAT                                                                     >  SRR4302545.573262/1‑94 (MQ=60)
                           CGACACGGTGGAAGATCGCGGAAGCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATC                                                                    <  SRR4302545.6021378/94‑1 (MQ=60)
                           CGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATC                                                                    >  SRR4302545.9244754/1‑94 (MQ=60)
                            GACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCG                                                                   <  SRR4302545.7275192/94‑1 (MQ=60)
                            GACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCG                                                                   <  SRR4302545.4833266/94‑1 (MQ=60)
                             ACACGGTGTAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGG                                                                  <  SRR4302545.1151270/94‑1 (MQ=60)
                             ACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGG                                                                  >  SRR4302545.8616639/1‑94 (MQ=60)
                             ACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGG                                                                  >  SRR4302545.9216381/1‑94 (MQ=60)
                             ACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGG                                                                  <  SRR4302545.9819430/94‑1 (MQ=60)
                                CGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTT                                                               <  SRR4302545.5851701/94‑1 (MQ=60)
                                 GGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTA                                                              <  SRR4302545.3731872/94‑1 (MQ=60)
                                 GGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTA                                                              >  SRR4302545.4791426/1‑94 (MQ=60)
                                 GGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTA                                                              <  SRR4302545.6285082/94‑1 (MQ=60)
                                 GGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTA                                                              <  SRR4302545.7205156/94‑1 (MQ=60)
                                 GGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTA                                                              <  SRR4302545.7205155/94‑1 (MQ=60)
                                  GTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAG                                                             <  SRR4302545.10548285/94‑1 (MQ=60)
                                   TGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGG                                                            <  SRR4302545.1507744/94‑1 (MQ=60)
                                   TGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGG                                                            <  SRR4302545.10100913/94‑1 (MQ=60)
                                   TGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGG                                                            <  SRR4302545.5593376/94‑1 (MQ=60)
                                   TGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATGGCCACTGAGTTCCTCTCCATCGAAGATATCGGGTTAGG                                                            >  SRR4302545.9761027/1‑94 (MQ=60)
                                     GAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACAGAAGCTGAAATTGCCACTGAGTTCTTCTACATCGATGATATCGGGGTAGGTG                                                          >  SRR4302545.7326052/1‑94 (MQ=60)
                                      AAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGA                                                         >  SRR4302545.10799923/1‑94 (MQ=60)
                                      AAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGA                                                         >  SRR4302545.7831917/1‑94 (MQ=60)
                                        GATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAA                                                       >  SRR4302545.2056370/1‑94 (MQ=60)
                                        GATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATAGGGTTAGGGGAAA                                                       >  SRR4302545.2321415/1‑94 (MQ=60)
                                         ATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAA                                                      <  SRR4302545.8610887/94‑1 (MQ=60)
                                          TCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAA                                                     <  SRR4302545.10866668/94‑1 (MQ=60)
                                           CGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAG                                                    <  SRR4302545.5078838/94‑1 (MQ=60)
                                           CGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAG                                                    >  SRR4302545.7541751/1‑94 (MQ=60)
                                           CGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAG                                                    >  SRR4302545.1510296/1‑94 (MQ=60)
                                           CGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAG                                                    >  SRR4302545.7839268/1‑94 (MQ=60)
                                           CGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAG                                                    <  SRR4302545.9414709/94‑1 (MQ=60)
                                           CGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAG                                                    >  SRR4302545.2050914/1‑94 (MQ=60)
                                             CGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGT                                                  <  SRR4302545.862260/94‑1 (MQ=60)
                                             CGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGT                                                  <  SRR4302545.6853950/94‑1 (MQ=60)
                                             CGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGT                                                  <  SRR4302545.6853949/94‑1 (MQ=60)
                                             CGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGACGATATCGGGTTAGGTGAAAAAGGT                                                  >  SRR4302545.5430406/1‑94 (MQ=60)
                                              GGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTC                                                 >  SRR4302545.3346510/1‑94 (MQ=60)
                                              GGAACCATTCAGTAGTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTC                                                 <  SRR4302545.6312538/94‑1 (MQ=60)
                                               GAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCA                                                >  SRR4302545.9185907/1‑94 (MQ=60)
                                                AACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAG                                               >  SRR4302545.5010406/1‑94 (MQ=60)
                                                AACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAG                                               >  SRR4302545.4750242/1‑94 (MQ=60)
                                                AACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAG                                               <  SRR4302545.7423534/94‑1 (MQ=60)
                                                 ACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGT                                              <  SRR4302545.10402917/94‑1 (MQ=60)
                                                  CCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTT                                             >  SRR4302545.4948964/1‑94 (MQ=60)
                                                  CCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTT                                             >  SRR4302545.3858129/1‑94 (MQ=60)
                                                  CCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTT                                             <  SRR4302545.7075189/94‑1 (MQ=60)
                                                  CCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTT                                             <  SRR4302545.8539149/94‑1 (MQ=60)
                                                  CCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTT                                             <  SRR4302545.3965677/94‑1 (MQ=60)
                                                  CCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGATAGGTGAAAAAGGTCAGTT                                             >  SRR4302545.398405/1‑94 (MQ=60)
                                                   CATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTC                                            <  SRR4302545.10002650/94‑1 (MQ=60)
                                                    ATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCA                                           >  SRR4302545.10079155/1‑94 (MQ=60)
                                                    ATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCA                                           >  SRR4302545.1203831/1‑94 (MQ=60)
                                                     TTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCAC                                          <  SRR4302545.3522938/94‑1 (MQ=60)
                                                     TTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGCGAAAAAGGTCAGTTCAC                                          >  SRR4302545.7458362/1‑94 (MQ=60)
                                                      TCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACG                                         <  SRR4302545.2142319/94‑1 (MQ=60)
                                                      TCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACG                                         <  SRR4302545.3662013/94‑1 (MQ=60)
                                                      TCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACG                                         >  SRR4302545.10333300/1‑94 (MQ=60)
                                                      TCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACG                                         <  SRR4302545.5678866/94‑1 (MQ=60)
                                                       TAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGG                                        <  SRR4302545.10321163/94‑1 (MQ=60)
                                                       CAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGG                                        >  SRR4302545.7165156/1‑94 (MQ=60)
                                                         GTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGAT                                      <  SRR4302545.3375217/94‑1 (MQ=60)
                                                          TATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGATCCTCTGCATCCATGATTTCGGGATAGTTGAAAAAGGTGAGTTCACGTATT                                     >  SRR4302545.2600081/1‑94 (MQ=55)
                                                           ATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTT                                    >  SRR4302545.443555/1‑94 (MQ=60)
                                                           ATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTT                                    >  SRR4302545.10499421/1‑94 (MQ=60)
                                                           ATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTT                                    <  SRR4302545.1030227/94‑1 (MQ=60)
                                                           ATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTT                                    <  SRR4302545.1238829/94‑1 (MQ=60)
                                                           ATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTT                                    <  SRR4302545.1097697/94‑1 (MQ=60)
                                                           ATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTT                                    >  SRR4302545.10217812/1‑94 (MQ=60)
                                                            TTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTA                                   >  SRR4302545.9372225/1‑94 (MQ=60)
                                                             TTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTAC                                  <  SRR4302545.293561/94‑1 (MQ=60)
                                                              TGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACA                                 >  SRR4302545.751220/1‑94 (MQ=60)
                                                              TGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACA                                 >  SRR4302545.444949/1‑94 (MQ=60)
                                                              TGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACA                                 >  SRR4302545.4625180/1‑94 (MQ=60)
                                                              TGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACA                                 >  SRR4302545.8452477/1‑94 (MQ=60)
                                                               GCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAG                                <  SRR4302545.161992/94‑1 (MQ=60)
                                                               GCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAG                                <  SRR4302545.5406561/94‑1 (MQ=60)
                                                               GCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAG                                >  SRR4302545.6548703/1‑94 (MQ=60)
                                                               GCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAG                                >  SRR4302545.2538734/1‑94 (MQ=60)
                                                               GCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAG                                >  SRR4302545.6718902/1‑94 (MQ=60)
                                                                CAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGG                               <  SRR4302545.6012892/94‑1 (MQ=60)
                                                                CAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGG                               <  SRR4302545.8505546/94‑1 (MQ=60)
                                                                CAGGACTGCGCAACGGAAGCGGAAATTGCAACTAAGTTCTTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTAaaga                               >  SRR4302545.9323868/1‑90 (MQ=60)
                                                                 AGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGA                              >  SRR4302545.6917120/1‑94 (MQ=60)
                                                                 AGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGA                              <  SRR4302545.2559807/94‑1 (MQ=60)
                                                                 AGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGGGAAAAAGGTCAGTTCACGGATTTACAGGT                              >  SRR4302545.8997630/1‑94 (MQ=60)
                                                                  GGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGTTTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGAT                             <  SRR4302545.541509/94‑1 (MQ=60)
                                                                  GGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGAT                             <  SRR4302545.7109820/94‑1 (MQ=60)
                                                                  GGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGAT                             <  SRR4302545.7423696/94‑1 (MQ=60)
                                                                   GACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATC                            <  SRR4302545.8768468/94‑1 (MQ=60)
                                                                   GACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATC                            <  SRR4302545.6440016/94‑1 (MQ=60)
                                                                   GACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATC                            <  SRR4302545.10547929/94‑1 (MQ=60)
                                                                   GACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATC                            >  SRR4302545.3880168/1‑94 (MQ=60)
                                                                     CTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAG                          >  SRR4302545.10821982/1‑94 (MQ=60)
                                                                     CTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAG                          <  SRR4302545.8980235/94‑1 (MQ=60)
                                                                     CTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAG                          <  SRR4302545.147815/94‑1 (MQ=60)
                                                                     CTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAG                          >  SRR4302545.2796825/1‑94 (MQ=60)
                                                                      TGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGG                         >  SRR4302545.3686045/1‑94 (MQ=60)
                                                                      TGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACAGCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGG                         <  SRR4302545.1923652/94‑1 (MQ=60)
                                                                      CGCGCAACGGAAGCTGAAATTGCCACGGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGG                         <  SRR4302545.6214912/94‑1 (MQ=60)
                                                                        CGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTA                       <  SRR4302545.10722918/94‑1 (MQ=60)
                                                                        CGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTA                       >  SRR4302545.1720502/1‑94 (MQ=60)
                                                                        CGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTA                       >  SRR4302545.4838672/1‑94 (MQ=60)
                                                                        CGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTA                       <  SRR4302545.5298016/94‑1 (MQ=60)
                                                                        CGCAACGGAAGCTGAAATTGACACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAACAAGGTCAGTTCACGGATTTACAGGATCAGGTA                       >  SRR4302545.9075634/1‑94 (MQ=60)
                                                                         GCAACGGAAGCTGAAATTGGCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAACAGGTAAGTTCACGGCTTTACAGGATCAGGTAA                      >  SRR4302545.3731750/1‑94 (MQ=60)
                                                                         GCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAA                      >  SRR4302545.5210381/1‑94 (MQ=60)
                                                                         GCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAA                      <  SRR4302545.6207305/94‑1 (MQ=60)
                                                                          CAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAAT                     >  SRR4302545.8961829/1‑94 (MQ=60)
                                                                           AACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATT                    <  SRR4302545.9962338/94‑1 (MQ=60)
                                                                           AACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATT                    <  SRR4302545.5860511/94‑1 (MQ=60)
                                                                           AACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATT                    >  SRR4302545.5132346/1‑94 (MQ=60)
                                                                           AACGGAAGCTGAAATTGCCACTGAGTTCCTCTACAACGATGATATCGTGTTAGGTGAAAAAGGTCAGTTCCCGGATTTACAGGATCAGGTAATT                    <  SRR4302545.5860187/94‑1 (MQ=60)
                                                                             CGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTC                  >  SRR4302545.2450967/1‑94 (MQ=60)
                                                                              GGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCC                 >  SRR4302545.10788152/1‑94 (MQ=60)
                                                                              GGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCC                 <  SRR4302545.981093/94‑1 (MQ=60)
                                                                              GGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCC                 <  SRR4302545.2635584/94‑1 (MQ=60)
                                                                               GAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCA                <  SRR4302545.10245912/94‑1 (MQ=60)
                                                                                AAGCTGAGATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAA               >  SRR4302545.8798378/1‑94 (MQ=60)
                                                                                AAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAA               >  SRR4302545.2950719/1‑94 (MQ=60)
                                                                                AAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAA               >  SRR4302545.7986154/1‑94 (MQ=60)
                                                                                 AGCTGAGATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAAC              <  SRR4302545.8735385/94‑1 (MQ=60)
                                                                                 AGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAAC              <  SRR4302545.2990455/94‑1 (MQ=60)
                                                                                   CTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCT            >  SRR4302545.2188324/1‑94 (MQ=60)
                                                                                   CTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCT            <  SRR4302545.8766798/94‑1 (MQ=60)
                                                                                   CTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCT            <  SRR4302545.2959159/94‑1 (MQ=60)
                                                                                   CTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCT            >  SRR4302545.4388587/1‑94 (MQ=60)
                                                                                   CTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCT            >  SRR4302545.10286774/1‑94 (MQ=60)
                                                                                    TGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTG           <  SRR4302545.9792816/94‑1 (MQ=60)
                                                                                    TGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTG           <  SRR4302545.5058294/94‑1 (MQ=60)
                                                                                    TGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTG           <  SRR4302545.2760416/94‑1 (MQ=60)
                                                                                    TGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTG           >  SRR4302545.2525573/1‑94 (MQ=60)
                                                                                     GAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTTACGGATTTACAGGATCAGGTAATTTCCAACCTGT          >  SRR4302545.9034581/1‑94 (MQ=60)
                                                                                     GAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGT          <  SRR4302545.2726552/94‑1 (MQ=60)
                                                                                     GAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGT          <  SRR4302545.9468825/94‑1 (MQ=60)
                                                                                     GAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGT          <  SRR4302545.9346253/94‑1 (MQ=60)
                                                                                      CAATTGCCACTGAGTTCCTCTACATCGATGATATAGGGTTAGGTGAAAAAGGGCAGTTGACGGATTTACAGGATCAAGTAATTTCCAACCTGTT         >  SRR4302545.10866760/1‑94 (MQ=60)
                                                                                      AAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTT         >  SRR4302545.1371747/1‑94 (MQ=60)
                                                                                      AAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTT         >  SRR4302545.1966460/1‑94 (MQ=60)
                                                                                      AAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTT         <  SRR4302545.5394727/94‑1 (MQ=60)
                                                                                       AATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTC        <  SRR4302545.8148713/94‑1 (MQ=60)
                                                                                       AATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTC        <  SRR4302545.8854035/94‑1 (MQ=60)
                                                                                        ATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCA       <  SRR4302545.2864186/94‑1 (MQ=60)
                                                                                        ATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCA       <  SRR4302545.10296849/94‑1 (MQ=60)
                                                                                          TGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAA     <  SRR4302545.8206000/94‑1 (MQ=60)
                                                                                          TGCCACTGAGTCCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAA     <  SRR4302545.8206001/94‑1 (MQ=60)
                                                                                           GCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAAC    >  SRR4302545.2694324/1‑94 (MQ=60)
                                                                                            CCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGGGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTACAACCTGTTCAAACT   >  SRR4302545.8887903/1‑94 (MQ=60)
                                                                                             CACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTG  >  SRR4302545.6394079/1‑94 (MQ=60)
                                                                                             CACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTG  <  SRR4302545.4152456/94‑1 (MQ=60)
                                                                                             |                                                                                             
CCAGTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTG  >  NC_000913/3178949‑3179135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑