Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,179,042	T→C	A202A (GCT→GCC)	ygiC →	putative synthetase/amidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,179,042	0	T	C	99.7%	1034.9 / NA	294	A202A (GCT→GCC)	ygiC	putative synthetase/amidase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (130/163); minor base A (1/0); total (131/163)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 295 total aligned reads displayed.

GTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAA  >  NC_000913/3178952‑3179131

  tgctgcATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTg                                                                                       <  1:1915959/93‑1 (MQ=255)

  tgctgcATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTg                                                                                       >  2:5354342/1‑93 (MQ=255)

   tctgcATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGa                                                                                      <  1:2084086/92‑1 (MQ=255)

      gcATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGtt                                                                                   >  1:641612/1‑93 (MQ=255)

      gcATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGtt                                                                                   <  1:5693389/93‑1 (MQ=255)

      gcATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGtt                                                                                   >  2:5547130/1‑93 (MQ=255)

         tctcACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCct                                                                                <  2:3404869/93‑1 (MQ=255)

         tctcACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCct                                                                                >  2:1558545/1‑93 (MQ=255)

          ctcACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCctc                                                                               >  1:3761223/1‑93 (MQ=255)

           tcACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCctct                                                                              >  1:3535030/1‑93 (MQ=255)

            cACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTa                                                                             >  2:3850904/1‑93 (MQ=255)

               ctgctgTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACAt                                                                          >  1:3346944/1‑93 (MQ=255)

               ctgctgTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACAt                                                                          >  1:4257341/1‑93 (MQ=255)

                 gctgTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCg                                                                        >  1:1335241/1‑93 (MQ=255)

                 gctgTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCg                                                                        >  2:572977/1‑93 (MQ=255)

                  ctgTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCga                                                                       <  1:24778/93‑1 (MQ=255)

                   tgTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCtat                                                                      <  2:825960/93‑1 (MQ=255)

                   tgTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCgat                                                                      <  2:1021491/93‑1 (MQ=255)

                   tgTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCgat                                                                      <  1:877157/93‑1 (MQ=255)

                     tCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCgatga                                                                    <  1:2722560/93‑1 (MQ=255)

                       gcgACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGata                                                                  <  1:3202574/93‑1 (MQ=255)

                        cgACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGatat                                                                 <  2:5651942/93‑1 (MQ=255)

                        cgACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGatat                                                                 <  1:2351127/93‑1 (MQ=255)

                         gACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATc                                                                <  2:3748845/93‑1 (MQ=255)

                          acacGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCg                                                               <  1:4045827/93‑1 (MQ=255)

                          acacGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCg                                                               >  1:6238612/1‑93 (MQ=255)

                            acGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCggg                                                             <  2:3598070/93‑1 (MQ=255)

                              ggTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGtt                                                           >  1:94892/1‑93 (MQ=255)

                              ggTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGtt                                                           >  1:1984835/1‑93 (MQ=255)

                               gTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTa                                                          >  1:4664454/1‑93 (MQ=255)

                                tGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAg                                                         >  2:2586674/1‑93 (MQ=255)

                                tGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAg                                                         <  1:5566532/93‑1 (MQ=255)

                                 ggAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAgg                                                        >  2:1133986/1‑93 (MQ=255)

                                  gAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGt                                                       <  2:5994970/93‑1 (MQ=255)

                                  gAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGt                                                       >  2:402084/1‑93 (MQ=255)

                                  gAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGt                                                       <  2:4280477/93‑1 (MQ=255)

                                  gAAGATCGCGCAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGt                                                       <  1:4966093/93‑1 (MQ=255)

                                    aGATCGCGGAACCATTCAGTATTTGCAGTACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGa                                                     <  2:1865282/93‑1 (MQ=255)

                                    aGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGa                                                     >  1:1436333/1‑93 (MQ=255)

                                    aGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGa                                                     <  2:6352100/93‑1 (MQ=255)

                                    aGATCGCGGAACCATTCAGAATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGa                                                     >  2:6308986/1‑93 (MQ=255)

                                     gATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGaa                                                    <  2:1402418/93‑1 (MQ=255)

                                     gATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGaa                                                    <  2:6179592/93‑1 (MQ=255)

                                      aTCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGaaa                                                   <  2:4233616/93‑1 (MQ=255)

                                       tCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGaaaa                                                  >  2:295849/1‑93 (MQ=255)

                                        cgcgGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGaaaaa                                                 <  1:5059592/93‑1 (MQ=255)

                                        cgcaGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGaaaaa                                                 <  2:763141/93‑1 (MQ=255)

                                         gcgGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAg                                                >  1:1580937/1‑93 (MQ=255)

                                          cgGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAgg                                               <  2:2882103/93‑1 (MQ=255)

                                           ggAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGt                                              >  2:3930658/1‑93 (MQ=255)

                                           ggAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGt                                              >  2:2383555/1‑93 (MQ=255)

                                           ggAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGt                                              >  1:79404/1‑93 (MQ=255)

                                           ggAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGt                                              >  1:3926320/1‑93 (MQ=255)

                                            gAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTc                                             <  1:6126319/93‑1 (MQ=255)

                                            gAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTc                                             <  2:2291736/93‑1 (MQ=255)

                                             aaCCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCa                                            <  1:356387/93‑1 (MQ=255)

                                               ccATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGATAGGTGAAAAAGGTCAGt                                          >  2:3970443/1‑93 (MQ=255)

                                                cATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGtt                                         >  2:5564461/1‑93 (MQ=255)

                                                 aTTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTc                                        >  2:5264969/1‑93 (MQ=255)

                                                 aTTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTc                                        >  1:3086249/1‑93 (MQ=255)

                                                 aTTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTc                                        >  2:516807/1‑93 (MQ=255)

                                                 aTTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTc                                        <  2:5004154/93‑1 (MQ=255)

                                                  ttCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCa                                       <  2:3620456/93‑1 (MQ=255)

                                                    cAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACg                                     <  1:2301708/93‑1 (MQ=255)

                                                    cAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACg                                     <  2:3192099/93‑1 (MQ=255)

                                                    cAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACg                                     <  2:3297909/93‑1 (MQ=255)

                                                     aGTATGTGCAGGACTGCGCAGCGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACgg                                    <  2:1376289/93‑1 (MQ=255)

                                                      gTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGa                                   <  1:1173099/93‑1 (MQ=255)

                                                      gTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGa                                   <  2:2726856/93‑1 (MQ=255)

                                                       tATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTATATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGAt                                  <  1:3455837/93‑1 (MQ=255)

                                                        aTTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGAtt                                 <  1:1809133/93‑1 (MQ=255)

                                                            gCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACa                             <  1:4640296/93‑1 (MQ=255)

                                                            gCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGGCAGCTCACGGATTTAcg                             >  2:4932073/1‑92 (MQ=255)

                                                             cAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAg                            <  2:2185340/93‑1 (MQ=255)

                                                             cAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAg                            <  2:2015579/93‑1 (MQ=255)

                                                               ggACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGa                          >  2:1923498/1‑93 (MQ=255)

                                                               ggACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGa                          >  1:36493/1‑93 (MQ=255)

                                                               ggACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGa                          <  1:3706399/93‑1 (MQ=255)

                                                               ggACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGa                          <  2:547634/93‑1 (MQ=255)

                                                               ggACTGCGCAACGGAAGCTGAAATTGCCACTGAGGTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGa                          >  2:6453142/1‑93 (MQ=255)

                                                                  cTGCTCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCa                       <  1:5151252/93‑1 (MQ=255)

                                                                   tGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAg                      <  2:1190575/93‑1 (MQ=255)

                                                                   tGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAg                      <  2:4829135/93‑1 (MQ=255)

                                                                   cgcgcAACGGAAGCTGAAATTGCCACTGAGTTCCACTACATCGATGATATCGTGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAg                      <  1:4831/92‑1 (MQ=255)

                                                                      gcAACGGAAGCTGGAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTa                   >  2:6474813/1‑93 (MQ=255)

                                                                        aaCGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAAt                 <  2:3558785/93‑1 (MQ=255)

                                                                        aaCGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAAt                 >  2:5081746/1‑93 (MQ=255)

                                                                         aCGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAAtt                >  1:6058904/1‑93 (MQ=255)

                                                                         aCGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAAtt                >  1:1089998/1‑93 (MQ=255)

                                                                          cGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAAttt               <  2:1278072/93‑1 (MQ=255)

                                                                           ggAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATATc              <  1:5365988/93‑1 (MQ=255)

                                                                             aaGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCa            >  1:2549445/1‑93 (MQ=255)

                                                                              aGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCaa           >  1:2282598/1‑93 (MQ=255)

                                                                               gCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAAc          >  2:4706951/1‑93 (MQ=255)

                                                                                cTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAAcc         >  1:218079/1‑93 (MQ=255)

                                                                                   aaaTTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGt      <  2:1755013/93‑1 (MQ=255)

                                                                                    aaTTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGtt     >  1:687488/1‑93 (MQ=255)

                                                                                     aTTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTc    >  1:4518381/1‑93 (MQ=255)

                                                                                     aTTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTc    <  1:4366147/93‑1 (MQ=255)

                                                                                      ttGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCa   <  1:5902443/93‑1 (MQ=255)

GTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAA  >  NC_000913/3178952‑3179131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCAGTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTG  >  NC_000913/3178949‑3179135

CCAGTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCC                                                                                               <  SRR4302537.3198456/94‑1 (MQ=60)

CCAGTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACGGCGCAACGGAAGCTGAAATTGCC                                                                                               <  SRR4302537.2131687/94‑1 (MQ=60)

 CAGTTGCTGCATCTCTCCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCA                                                                                              <  SRR4302537.6379320/94‑1 (MQ=60)

 CAGTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCA                                                                                              <  SRR4302537.100138/94‑1 (MQ=60)

 CAGTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCA                                                                                              <  SRR4302537.4733467/94‑1 (MQ=60)

 CAGTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCA                                                                                              <  SRR4302537.4752647/94‑1 (MQ=60)

  AGTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCAC                                                                                             <  SRR4302537.3346242/94‑1 (MQ=60)

   GTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACT                                                                                            >  SRR4302537.4429330/1‑94 (MQ=60)

   GTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACT                                                                                            <  SRR4302537.3605512/94‑1 (MQ=60)

     TGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGA                                                                                          <  SRR4302537.702065/94‑1 (MQ=60)

     TGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGA                                                                                          <  SRR4302537.5985502/94‑1 (MQ=60)

     TGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGA                                                                                          <  SRR4302537.1929255/94‑1 (MQ=60)

      TCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAG                                                                                         <  SRR4302537.2098771/94‑1 (MQ=60)

       CTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGT                                                                                        >  SRR4302537.2212328/1‑94 (MQ=60)

       CTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGT                                                                                        >  SRR4302537.4351636/1‑94 (MQ=60)

       CTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGT                                                                                        >  SRR4302537.3117993/1‑94 (MQ=60)

        TGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTT                                                                                       <  SRR4302537.1822225/94‑1 (MQ=60)

        TGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTT                                                                                       >  SRR4302537.195131/1‑94 (MQ=60)

        TGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTT                                                                                       <  SRR4302537.1853670/94‑1 (MQ=60)

        TGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTT                                                                                       <  SRR4302537.4739052/94‑1 (MQ=60)

        TGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTT                                                                                       >  SRR4302537.68140/1‑94 (MQ=60)

        TGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTT                                                                                       >  SRR4302537.1777642/1‑94 (MQ=60)

        TGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTT                                                                                       >  SRR4302537.645984/1‑94 (MQ=60)

        TGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTT                                                                                       <  SRR4302537.464240/94‑1 (MQ=60)

         GCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTC                                                                                      <  SRR4302537.5732328/94‑1 (MQ=60)

          CATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCC                                                                                     >  SRR4302537.843083/1‑94 (MQ=60)

           ATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCTCTGAGTTCCT                                                                                    <  SRR4302537.6514775/94‑1 (MQ=60)

           ATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCT                                                                                    >  SRR4302537.3859975/1‑94 (MQ=60)

            TCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTC                                                                                   >  SRR4302537.3787631/1‑94 (MQ=60)

            TCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTC                                                                                   >  SRR4302537.3701184/1‑94 (MQ=60)

             CTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCT                                                                                  >  SRR4302537.3560006/1‑94 (MQ=60)

             CTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCT                                                                                  <  SRR4302537.2486980/94‑1 (MQ=60)

               CACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTAC                                                                                <  SRR4302537.6224844/94‑1 (MQ=60)

                CCCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACA                                                                               <  SRR4302537.4300529/94‑1 (MQ=60)

                ACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACA                                                                               <  SRR4302537.1142602/94‑1 (MQ=60)

                ACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACA                                                                               >  SRR4302537.5093641/1‑94 (MQ=60)

                 CCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACAT                                                                              >  SRR4302537.3370639/1‑94 (MQ=60)

                 CCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACAT                                                                              >  SRR4302537.4286929/1‑94 (MQ=60)

                  CTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATC                                                                             >  SRR4302537.2825452/1‑94 (MQ=60)

                   TGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCG                                                                            >  SRR4302537.1344543/1‑94 (MQ=60)

                   TGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCG                                                                            >  SRR4302537.5770554/1‑94 (MQ=60)

                    GCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGA                                                                           >  SRR4302537.3500876/1‑94 (MQ=60)

                     CTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGAT                                                                          <  SRR4302537.24974/94‑1 (MQ=60)

                      TGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATG                                                                         <  SRR4302537.3755970/94‑1 (MQ=60)

                      TGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATG                                                                         <  SRR4302537.1525557/94‑1 (MQ=60)

                      TGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATG                                                                         <  SRR4302537.883191/94‑1 (MQ=60)

                       GTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGA                                                                        <  SRR4302537.4508673/94‑1 (MQ=60)

                        TCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGAT                                                                       <  SRR4302537.2741574/94‑1 (MQ=60)

                        TCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGAT                                                                       <  SRR4302537.458629/94‑1 (MQ=60)

                        TCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGAT                                                                       <  SRR4302537.5177101/94‑1 (MQ=60)

                          GCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATAT                                                                     <  SRR4302537.3225089/94‑1 (MQ=60)

                          GCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATAT                                                                     >  SRR4302537.3225263/1‑94 (MQ=60)

                           CGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATC                                                                    <  SRR4302537.2367680/94‑1 (MQ=60)

                            GACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCG                                                                   >  SRR4302537.4775098/1‑94 (MQ=60)

                            GACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCG                                                                   >  SRR4302537.6281506/1‑94 (MQ=60)

                            GACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCG                                                                   >  SRR4302537.4063543/1‑94 (MQ=60)

                            GACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCG                                                                   <  SRR4302537.3755737/94‑1 (MQ=60)

                             ACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGG                                                                  <  SRR4302537.4074116/94‑1 (MQ=60)

                              CACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGG                                                                 <  SRR4302537.4063584/94‑1 (MQ=60)

                              CACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGG                                                                 <  SRR4302537.6393853/94‑1 (MQ=60)

                                CGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTT                                                               >  SRR4302537.1998628/1‑94 (MQ=60)

                                CGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTT                                                               >  SRR4302537.95585/1‑94 (MQ=60)

                                CGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTT                                                               >  SRR4302537.5119640/1‑94 (MQ=60)

                                CGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTT                                                               >  SRR4302537.5250793/1‑94 (MQ=60)

                                 GGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTA                                                              >  SRR4302537.4696692/1‑94 (MQ=60)

                                 GGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTA                                                              >  SRR4302537.2342289/1‑94 (MQ=60)

                                   TGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGG                                                            <  SRR4302537.5604579/94‑1 (MQ=60)

                                     GAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG                                                          <  SRR4302537.4783608/94‑1 (MQ=60)

                                     GAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG                                                          <  SRR4302537.1803102/94‑1 (MQ=60)

                                     GAAGATCGCGCAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTG                                                          <  SRR4302537.5000321/94‑1 (MQ=60)

                                      AAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGA                                                         >  SRR4302537.1446320/1‑94 (MQ=60)

                                       AGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAA                                                        >  SRR4302537.2070995/1‑94 (MQ=60)

                                        GATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAA                                                       >  SRR4302537.5456639/1‑94 (MQ=60)

                                          TCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAA                                                     <  SRR4302537.3122012/94‑1 (MQ=60)

                                           CGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAG                                                    >  SRR4302537.1591986/1‑94 (MQ=60)

                                           CGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAG                                                    <  SRR4302537.5094423/94‑1 (MQ=60)

                                           CGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAG                                                    <  SRR4302537.1459963/94‑1 (MQ=60)

                                            GCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGG                                                   <  SRR4302537.2351552/94‑1 (MQ=60)

                                            GCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGG                                                   >  SRR4302537.3449307/1‑94 (MQ=60)

                                             CGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGT                                                  <  SRR4302537.6432089/94‑1 (MQ=60)

                                             CGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGT                                                  >  SRR4302537.79987/1‑94 (MQ=60)

                                             CGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGT                                                  >  SRR4302537.3953752/1‑94 (MQ=60)

                                              GGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTC                                                 >  SRR4302537.2939265/1‑94 (MQ=60)

                                              GGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTC                                                 >  SRR4302537.5709158/1‑94 (MQ=60)

                                              GGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTC                                                 <  SRR4302537.227697/94‑1 (MQ=60)

                                               GAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCA                                                >  SRR4302537.3348968/1‑94 (MQ=60)

                                               GAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCA                                                <  SRR4302537.6035633/94‑1 (MQ=60)

                                               GAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCA                                                >  SRR4302537.1399661/1‑94 (MQ=60)

                                               GAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCA                                                <  SRR4302537.6168427/94‑1 (MQ=60)

                                               GAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCA                                                <  SRR4302537.424881/94‑1 (MQ=60)

                                                AACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAG                                               <  SRR4302537.358864/94‑1 (MQ=60)

                                                AACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAG                                               >  SRR4302537.618392/1‑94 (MQ=60)

                                                  CCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTT                                             <  SRR4302537.2658359/94‑1 (MQ=60)

                                                  CCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTT                                             <  SRR4302537.4873086/94‑1 (MQ=60)

                                                   CATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTC                                            >  SRR4302537.3647941/1‑94 (MQ=60)

                                                   CATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTC                                            >  SRR4302537.3107753/1‑94 (MQ=60)

                                                   CATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTC                                            >  SRR4302537.3729754/1‑94 (MQ=60)

                                                    ATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCA                                           <  SRR4302537.5759599/94‑1 (MQ=60)

                                                     TTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCAC                                          <  SRR4302537.5679636/94‑1 (MQ=60)

                                                     TTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCAC                                          >  SRR4302537.3421614/1‑94 (MQ=60)

                                                      TCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACG                                         <  SRR4302537.1932430/94‑1 (MQ=60)

                                                      TCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACG                                         <  SRR4302537.962591/94‑1 (MQ=60)

                                                       CAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGG                                        <  SRR4302537.2317955/94‑1 (MQ=60)

                                                        AGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGA                                       >  SRR4302537.2302293/1‑94 (MQ=60)

                                                         GTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGAT                                      <  SRR4302537.1181261/94‑1 (MQ=60)

                                                         GTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGAT                                      <  SRR4302537.1419670/94‑1 (MQ=60)

                                                         GTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGAT                                      <  SRR4302537.5211/94‑1 (MQ=60)

                                                          TATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTATATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATT                                     <  SRR4302537.3480275/94‑1 (MQ=60)

                                                          TATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATT                                     <  SRR4302537.1332498/94‑1 (MQ=60)

                                                          TATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATT                                     >  SRR4302537.840271/1‑94 (MQ=60)

                                                           ATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTT                                    <  SRR4302537.1821752/94‑1 (MQ=60)

                                                           ATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTT                                    >  SRR4302537.2103980/1‑94 (MQ=60)

                                                             TTGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTAC                                  <  SRR4302537.6143608/94‑1 (MQ=60)

                                                              TGCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACA                                 >  SRR4302537.4681190/1‑94 (MQ=60)

                                                               GCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAG                                <  SRR4302537.4672383/94‑1 (MQ=60)

                                                               GCAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAG                                <  SRR4302537.2565476/94‑1 (MQ=60)

                                                                CAGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGG                               <  SRR4302537.3567620/94‑1 (MQ=60)

                                                                 AGGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGA                              >  SRR4302537.36771/1‑94 (MQ=60)

                                                                  GGACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGAT                             <  SRR4302537.3732465/94‑1 (MQ=60)

                                                                    ACTGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCA                           <  SRR4302537.2306771/94‑1 (MQ=60)

                                                                     CTGCTCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAG                          <  SRR4302537.5186748/94‑1 (MQ=60)

                                                                      TGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGG                         >  SRR4302537.4689689/1‑94 (MQ=60)

                                                                      CGCGCAACGGAAGCTGAAATTGCCACTGAGTTCCACTACATCGATGATATCGTGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGG                         <  SRR4302537.4871/94‑1 (MQ=60)

                                                                       GCGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGT                        <  SRR4302537.2046323/94‑1 (MQ=60)

                                                                        CGCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTA                       <  SRR4302537.945831/94‑1 (MQ=60)

                                                                         GCAACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAA                      >  SRR4302537.728988/1‑94 (MQ=60)

                                                                           AACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATT                    >  SRR4302537.6100529/1‑94 (MQ=60)

                                                                           AACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATT                    >  SRR4302537.1097524/1‑94 (MQ=60)

                                                                           AACGGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATT                    <  SRR4302537.4877251/94‑1 (MQ=60)

                                                                              GGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCC                 <  SRR4302537.2133204/94‑1 (MQ=60)

                                                                              GGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCC                 >  SRR4302537.2531712/1‑94 (MQ=60)

                                                                              GGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCC                 <  SRR4302537.3505998/94‑1 (MQ=60)

                                                                              GGAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATATCC                 <  SRR4302537.5402705/94‑1 (MQ=60)

                                                                               GAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCA                >  SRR4302537.5022153/1‑94 (MQ=60)

                                                                               GAAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCA                >  SRR4302537.2567305/1‑94 (MQ=60)

                                                                                AAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAA               <  SRR4302537.1300745/94‑1 (MQ=60)

                                                                                AAGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAA               >  SRR4302537.2298703/1‑94 (MQ=60)

                                                                                 AGCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAAC              >  SRR4302537.5493276/1‑94 (MQ=60)

                                                                                  GCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACC             >  SRR4302537.384526/1‑94 (MQ=60)

                                                                                  GCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACC             >  SRR4302537.219633/1‑94 (MQ=60)

                                                                                  GCTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACC             <  SRR4302537.901248/94‑1 (MQ=60)

                                                                                   CTGAAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCT            <  SRR4302537.2651011/94‑1 (MQ=60)

                                                                                      AAATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTT         >  SRR4302537.692202/1‑94 (MQ=60)

                                                                                       AATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTC        >  SRR4302537.4549636/1‑94 (MQ=60)

                                                                                        ATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCA       <  SRR4302537.4137497/94‑1 (MQ=60)

                                                                                        ATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCA       <  SRR4302537.4396332/94‑1 (MQ=60)

                                                                                        ATTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCA       <  SRR4302537.2392934/94‑1 (MQ=60)

                                                                                         TTGCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAA      <  SRR4302537.5942943/94‑1 (MQ=60)

                                                                                           GCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAAC    <  SRR4302537.2994542/94‑1 (MQ=60)

                                                                                           GCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAAC    >  SRR4302537.5395430/1‑94 (MQ=60)

                                                                                           GCCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAAC    >  SRR4302537.549944/1‑94 (MQ=60)

                                                                                            CCACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACT   <  SRR4302537.4102212/94‑1 (MQ=60)

                                                                                             CACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTG  <  SRR4302537.3387409/94‑1 (MQ=60)

                                                                                             CACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTG  <  SRR4302537.6223900/94‑1 (MQ=60)

                                                                                             CACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTG  >  SRR4302537.5758791/1‑94 (MQ=60)

CCAGTTGCTGCATCTCACCTGCTGTCGCGACACGGTGGAAGATCGCGGAACCATTCAGTATTTGCAGGACTGCGCAACGGAAGCTGAAATTGCTACTGAGTTCCTCTACATCGATGATATCGGGTTAGGTGAAAAAGGTCAGTTCACGGATTTACAGGATCAGGTAATTTCCAACCTGTTCAAACTG  >  NC_000913/3178949‑3179135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`