Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,205,532 T→C L174P (CTG→CCG)  ttdB → L‑tartrate dehydratase subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,205,5320TC98.5% 1379.0 / 3.8 413L174P (CTG→CCG) ttdBL‑tartrate dehydratase subunit B
Reads supporting (aligned to +/- strand):  ref base T (3/3);  new base C (194/212);  total (198/215)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

Only 100 of 428 total aligned reads displayed.

ACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTC  >  NC_000913/3205441‑3205622
                                                                                           |                                                                                          
aCAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGa                                                                                          >  1:3001563/1‑94 (MQ=255)
  agagCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATa                                                                                        <  1:5441764/94‑1 (MQ=255)
   gagCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAg                                                                                       <  2:9643055/94‑1 (MQ=255)
   gagCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAg                                                                                       <  2:5495142/94‑1 (MQ=255)
     gCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGcc                                                                                     >  1:5276885/1‑94 (MQ=255)
     gCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGcc                                                                                     <  1:6714880/94‑1 (MQ=255)
     gCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGcc                                                                                     >  1:8322385/1‑94 (MQ=255)
      cTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCTGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCCATAGCCg                                                                                    >  2:4512916/1‑94 (MQ=255)
      cTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCg                                                                                    >  2:10376044/1‑94 (MQ=255)
        cGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCTACGGCAACAACCCGATAGCCGaa                                                                                  >  2:5738253/1‑94 (MQ=255)
            aTGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACa                                                                              <  1:10385994/94‑1 (MQ=255)
            aTGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACa                                                                              >  2:9006029/1‑94 (MQ=255)
             tGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACaa                                                                             >  2:1326639/1‑94 (MQ=255)
              gCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACaaa                                                                            >  1:7917012/1‑94 (MQ=255)
               ccGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACaaaa                                                                           >  1:6022833/1‑94 (MQ=255)
               ccGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACaaaa                                                                           >  2:2558033/1‑94 (MQ=255)
                cGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACaaaaa                                                                          >  2:1112077/1‑94 (MQ=255)
                 ggAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCCACAACCCGATAGCCGACAACAAACAg                                                                         >  2:1671188/1‑94 (MQ=255)
                  gAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGc                                                                        <  2:5377811/94‑1 (MQ=255)
                   aGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCt                                                                       <  2:4372169/94‑1 (MQ=255)
                     tCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGt                                                                     <  2:6555941/94‑1 (MQ=255)
                      cACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGtt                                                                    >  1:1088565/1‑94 (MQ=255)
                      cACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGtt                                                                    <  1:2279018/94‑1 (MQ=255)
                       aCTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTc                                                                   >  2:5843698/1‑94 (MQ=255)
                        cTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCg                                                                  <  1:6918753/94‑1 (MQ=255)
                           tgGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCg                                                               <  1:4039982/94‑1 (MQ=255)
                            gggTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGa                                                              <  2:8243967/94‑1 (MQ=255)
                             ggTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCGATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGaa                                                             <  2:2159314/94‑1 (MQ=255)
                              gTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAAc                                                            <  2:8664070/94‑1 (MQ=255)
                               tCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAAcg                                                           <  2:1883811/94‑1 (MQ=255)
                                cTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAAcgc                                                          >  2:10177253/1‑94 (MQ=255)
                                cTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAAtgc                                                          >  1:9370043/1‑94 (MQ=255)
                                cTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAAcgc                                                          >  2:1368267/1‑94 (MQ=255)
                                cTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAAcgc                                                          >  1:147460/1‑94 (MQ=255)
                                cTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAAcgc                                                          <  1:8480805/94‑1 (MQ=255)
                                 tGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAAcgcc                                                         <  2:2979071/94‑1 (MQ=255)
                                  tccGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAAcgccg                                                        <  2:7951520/93‑1 (MQ=255)
                                  gCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAAcgccg                                                        <  2:10427679/94‑1 (MQ=255)
                                  gCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAAcgccg                                                        >  2:8440993/1‑94 (MQ=255)
                                  gCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAAcgccg                                                        <  1:4452397/94‑1 (MQ=255)
                                   ccGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAAcgccgc                                                       <  1:994611/94‑1 (MQ=255)
                                    cGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCcgcg                                                      >  2:7119940/1‑94 (MQ=255)
                                     gggTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGa                                                     >  1:9796934/1‑94 (MQ=255)
                                       gTCAAAGAGTTCGGCCCTCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATc                                                   <  2:4872139/94‑1 (MQ=255)
                                       gTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATc                                                   <  2:3728098/94‑1 (MQ=255)
                                         cAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATccc                                                 >  2:856914/1‑94 (MQ=255)
                                          aaaGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCa                                                <  1:10019935/94‑1 (MQ=255)
                                           aaGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCTGCGATCCCAt                                               <  1:7576065/94‑1 (MQ=255)
                                            agagTTCGGCCCGCTGATTGTCTCTATTGATACCCAGGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATc                                              <  1:10480674/94‑1 (MQ=255)
                                                ttCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTgg                                          <  1:9997419/94‑1 (MQ=255)
                                                 tCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGa                                         <  2:7688875/94‑1 (MQ=255)
                                                 tCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGa                                         >  1:8182848/1‑94 (MQ=255)
                                                  cGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGaa                                        <  2:9101309/94‑1 (MQ=255)
                                                   ggCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAag                                       >  2:6132572/1‑94 (MQ=255)
                                                   ggCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAag                                       >  1:4632107/1‑94 (MQ=255)
                                                   ggCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAag                                       <  1:10234714/94‑1 (MQ=255)
                                                    gCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAaga                                      <  2:8086306/94‑1 (MQ=255)
                                                    gCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGCGGAaga                                      >  1:3482650/1‑94 (MQ=255)
                                                     cccGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAagag                                     <  1:334020/94‑1 (MQ=255)
                                                       cGCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGAt                                   <  2:2813627/94‑1 (MQ=255)
                                                        gCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATc                                  <  1:5098452/94‑1 (MQ=255)
                                                        gCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATc                                  <  2:10068280/94‑1 (MQ=255)
                                                        gCTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATc                                  >  2:6213955/1‑94 (MQ=255)
                                                         cTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCt                                 <  2:6889573/94‑1 (MQ=255)
                                                         cTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCt                                 >  2:3526199/1‑94 (MQ=255)
                                                         cTGATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCt                                 <  2:2388034/94‑1 (MQ=255)
                                                           gATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGc                               >  1:1865856/1‑94 (MQ=255)
                                                           gATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGc                               <  1:1934810/94‑1 (MQ=255)
                                                           gATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGc                               >  1:6634254/1‑94 (MQ=255)
                                                           gATTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGc                               <  2:6435531/94‑1 (MQ=255)
                                                            aTTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCg                              <  1:4155314/94‑1 (MQ=255)
                                                            aTTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCg                              <  2:788838/94‑1 (MQ=255)
                                                            aTTGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCg                              <  2:730238/94‑1 (MQ=255)
                                                             ttGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGa                             >  1:8682604/1‑94 (MQ=255)
                                                              tGTCTCTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAg                            >  2:1920306/1‑94 (MQ=255)
                                                               gTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGc                           >  1:7298565/1‑94 (MQ=255)
                                                                tctcTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCa                          >  2:3888085/1‑94 (MQ=255)
                                                                tctcTATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCa                          <  1:9650909/94‑1 (MQ=255)
                                                                 ctctATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCAc                         <  1:3731379/94‑1 (MQ=255)
                                                                  tctATTGATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACg                        <  2:3437124/94‑1 (MQ=255)
                                                                        gATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCAtt                  >  2:1165782/1‑94 (MQ=255)
                                                                        gATACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCAtt                  >  1:5631938/1‑94 (MQ=255)
                                                                         aTACCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTa                 <  1:1726335/94‑1 (MQ=255)
                                                                           aCCCACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACa               >  1:8961828/1‑94 (MQ=255)
                                                                              cACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCa            <  2:6657202/94‑1 (MQ=255)
                                                                              cACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCa            >  2:7557306/1‑94 (MQ=255)
                                                                              cACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCa            >  2:9192029/1‑94 (MQ=255)
                                                                              cACGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCa            <  2:9373638/94‑1 (MQ=255)
                                                                               aCGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCaa           >  1:2185923/1‑94 (MQ=255)
                                                                               aCGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCaa           <  2:3036327/94‑1 (MQ=255)
                                                                                cGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCaaa          >  2:5142138/1‑94 (MQ=255)
                                                                                cGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCaaa          >  2:4216927/1‑94 (MQ=255)
                                                                                cGGCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCaaa          <  1:9169997/94‑1 (MQ=255)
                                                                                 ggCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAAt         >  1:2612781/1‑94 (MQ=255)
                                                                                 ggCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAAt         >  1:7757100/1‑94 (MQ=255)
                                                                                  gCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATa        >  1:3545123/1‑94 (MQ=255)
                                                                                  gCAACAACCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATa        <  1:4982515/94‑1 (MQ=255)
                                                                                     acaacCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAAcc     <  1:1229166/94‑1 (MQ=255)
                                                                                      caacCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAAccc    >  2:2850959/1‑94 (MQ=255)
                                                                                        acCCGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTc  >  1:1336682/1‑94 (MQ=255)
                                                                                           |                                                                                          
ACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTC  >  NC_000913/3205441‑3205622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 309 total aligned reads displayed.
AAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGA  >  NC_000913/3208327‑3208513
                                                                                             |                                                                                             
AAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCG                                                                                               >  SRR4302545.1113482/1‑94 (MQ=60)
AAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCG                                                                                               <  SRR4302545.6766419/94‑1 (MQ=60)
AAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCG                                                                                               >  SRR4302545.8438461/1‑94 (MQ=60)
AAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCG                                                                                               >  SRR4302545.8500782/1‑94 (MQ=60)
AAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCG                                                                                               >  SRR4302545.1346310/1‑94 (MQ=60)
AAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCG                                                                                               >  SRR4302545.5047762/1‑94 (MQ=60)
 AAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTTGCGTTGATGCCAGCGGCAAACCCAATTAATCAAAGGTGAGAGGCGC                                                                                              >  SRR4302545.114212/1‑94 (MQ=60)
 AAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGC                                                                                              >  SRR4302545.10161290/1‑94 (MQ=60)
 AAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGC                                                                                              >  SRR4302545.3199397/1‑94 (MQ=60)
 AAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGC                                                                                              <  SRR4302545.10387441/94‑1 (MQ=60)
  AGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAATCCGAATTAATCAAAGGTGAGAGGCGCA                                                                                             >  SRR4302545.1281662/1‑94 (MQ=60)
  AGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCA                                                                                             <  SRR4302545.3550250/94‑1 (MQ=60)
   GCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCGGCGGCAAACCGAATTAACCAAAGGTGAGAGGCGCAT                                                                                            <  SRR4302545.6802715/94‑1 (MQ=60)
   GCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCAT                                                                                            <  SRR4302545.2283406/94‑1 (MQ=60)
    CAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATG                                                                                           <  SRR4302545.7845200/94‑1 (MQ=60)
    CAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATG                                                                                           <  SRR4302545.1494316/94‑1 (MQ=60)
    CAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATG                                                                                           >  SRR4302545.6087407/1‑94 (MQ=60)
    CAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATG                                                                                           >  SRR4302545.6087408/1‑94 (MQ=60)
    CAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATG                                                                                           <  SRR4302545.10710686/94‑1 (MQ=60)
     AGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGC                                                                                          >  SRR4302545.1287782/1‑94 (MQ=60)
     AGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGC                                                                                          >  SRR4302545.2636638/1‑94 (MQ=60)
      GTAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCC                                                                                         <  SRR4302545.4255172/94‑1 (MQ=60)
       CAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCG                                                                                        >  SRR4302545.3932902/1‑94 (MQ=60)
       CAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCG                                                                                        >  SRR4302545.5771704/1‑94 (MQ=60)
        AGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGG                                                                                       >  SRR4302545.8034692/1‑94 (MQ=60)
         GCAATTGCAGTACAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGT                                                                                      <  SRR4302545.9643138/94‑1 (MQ=60)
          CAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTA                                                                                     >  SRR4302545.287260/1‑94 (MQ=60)
           AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAA                                                                                    <  SRR4302545.2086234/94‑1 (MQ=60)
           AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAA                                                                                    >  SRR4302545.9060018/1‑94 (MQ=60)
           AATGGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAA                                                                                    >  SRR4302545.9683162/1‑94 (MQ=60)
              TGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTA                                                                                 <  SRR4302545.10002319/94‑1 (MQ=60)
               TCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAA                                                                                >  SRR4302545.7048296/1‑94 (MQ=60)
               GCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAA                                                                                <  SRR4302545.6308809/94‑1 (MQ=60)
               GCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAA                                                                                >  SRR4302545.8545370/1‑94 (MQ=60)
                 CGTAAAATTCCGCACCATGTTGAAATAAGCTGGCATTGATGCCAGCGTCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAG                                                                              <  SRR4302545.781933/94‑1 (MQ=60)
                 AGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAG                                                                              >  SRR4302545.10714512/1‑94 (MQ=60)
                    AAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTAC                                                                           <  SRR4302545.8994596/94‑1 (MQ=60)
                    AAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTAC                                                                           >  SRR4302545.8794593/1‑94 (MQ=60)
                    AAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTAC                                                                           >  SRR4302545.8267147/1‑94 (MQ=60)
                     AAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACG                                                                          <  SRR4302545.6624886/94‑1 (MQ=60)
                     AAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACG                                                                          >  SRR4302545.9824198/1‑94 (MQ=60)
                      AATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAGTTAAAGTAAGT                                                                         >  SRR4302545.4461093/1‑94 (MQ=60)
                      AATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGT                                                                         <  SRR4302545.2907785/94‑1 (MQ=60)
                       ATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTG                                                                        >  SRR4302545.7790566/1‑94 (MQ=60)
                       ATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTG                                                                        >  SRR4302545.3288904/1‑94 (MQ=60)
                       ATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTG                                                                        >  SRR4302545.10078873/1‑94 (MQ=60)
                        TTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGA                                                                       >  SRR4302545.5428497/1‑94 (MQ=60)
                         TCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAA                                                                      <  SRR4302545.9494502/94‑1 (MQ=60)
                          CCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAA                                                                     >  SRR4302545.461705/1‑94 (MQ=60)
                           CGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAC                                                                    >  SRR4302545.3501386/1‑94 (MQ=60)
                           CGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAA                                                                    <  SRR4302545.6542887/94‑1 (MQ=60)
                            GCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAAC                                                                   <  SRR4302545.7989071/94‑1 (MQ=60)
                            GCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAAC                                                                   >  SRR4302545.4463585/1‑94 (MQ=60)
                            GCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGCCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAAC                                                                   <  SRR4302545.6802862/94‑1 (MQ=60)
                             CACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACG                                                                  <  SRR4302545.8612425/94‑1 (MQ=60)
                             CACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACG                                                                  >  SRR4302545.5156302/1‑94 (MQ=60)
                             CACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACG                                                                  >  SRR4302545.3394204/1‑94 (MQ=60)
                             CACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACG                                                                  >  SRR4302545.1801774/1‑94 (MQ=60)
                             AACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGACAACG                                                                  >  SRR4302545.10738611/1‑94 (MQ=60)
                               CCATTTTTAAATAAACTGGCGTTGGTGCCCGGGGCAAAACGAAATAATCAAAGGTGCGAGGGGCATGCCGGTAATTAAAGTACGTGAAAAAGAG                                                                >  SRR4302545.230065/1‑94 (MQ=34)
                               CCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAG                                                                <  SRR4302545.1920016/94‑1 (MQ=60)
                               CCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAG                                                                >  SRR4302545.9320028/1‑94 (MQ=60)
                                CATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGATAGGAGCATGCCGGTAATTAAAGGACGTGAAAACGAGC                                                               >  SRR4302545.9135832/1‑94 (MQ=60)
                                CATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGC                                                               <  SRR4302545.3283091/94‑1 (MQ=60)
                                CATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGC                                                               >  SRR4302545.3862351/1‑94 (MQ=60)
                                CATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGC                                                               <  SRR4302545.5979646/94‑1 (MQ=60)
                                CATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGC                                                               <  SRR4302545.1652176/94‑1 (MQ=60)
                                 ATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCC                                                              >  SRR4302545.5860330/1‑94 (MQ=60)
                                 ATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCC                                                              >  SRR4302545.6692004/1‑94 (MQ=60)
                                 ATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCC                                                              <  SRR4302545.10096346/94‑1 (MQ=60)
                                   tataAAATAAGCTGGCGTCAATGCAAGCGGCAAACCGAATTAAACAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGT                                                            <  SRR4302545.7002463/90‑1 (MQ=60)
                                   TTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGT                                                            <  SRR4302545.1324071/94‑1 (MQ=60)
                                    taaaaagAAGCTGGCGTTGAGGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTT                                                           <  SRR4302545.2388837/87‑1 (MQ=60)
                                    TTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTT                                                           >  SRR4302545.4788814/1‑94 (MQ=60)
                                    TTAAAATAAGCTGGCGTTGATGCTAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTT                                                           <  SRR4302545.5968106/94‑1 (MQ=60)
                                     TGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTC                                                          <  SRR4302545.3450072/94‑1 (MQ=60)
                                      GAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCG                                                         >  SRR4302545.1920644/1‑94 (MQ=60)
                                      GAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCG                                                         >  SRR4302545.5001181/1‑94 (MQ=60)
                                      GAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCG                                                         <  SRR4302545.5594394/94‑1 (MQ=60)
                                      GAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCG                                                         >  SRR4302545.8494698/1‑94 (MQ=60)
                                      GAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCG                                                         >  SRR4302545.8453952/1‑94 (MQ=60)
                                       AAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGA                                                        <  SRR4302545.5524330/94‑1 (MQ=60)
                                         ATAAGCTGGCGTTGATGCCAGCGGCAACCCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACG                                                      <  SRR4302545.5069541/94‑1 (MQ=60)
                                         ATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACG                                                      >  SRR4302545.9400355/1‑94 (MQ=60)
                                         ATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACG                                                      >  SRR4302545.4875001/1‑94 (MQ=60)
                                         ATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACG                                                      <  SRR4302545.308062/94‑1 (MQ=60)
                                         ATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACG                                                      >  SRR4302545.3521593/1‑94 (MQ=60)
                                         ATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACG                                                      <  SRR4302545.7617832/94‑1 (MQ=60)
                                           AAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTA                                                    <  SRR4302545.9714332/94‑1 (MQ=60)
                                           AAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTA                                                    >  SRR4302545.6978807/1‑94 (MQ=60)
                                           AAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTA                                                    >  SRR4302545.2666958/1‑94 (MQ=60)
                                           AAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTA                                                    >  SRR4302545.6006056/1‑94 (MQ=60)
                                            AGCTGGCGTGGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTGG                                                   <  SRR4302545.7542284/94‑1 (MQ=60)
                                             GCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGC                                                  >  SRR4302545.5610659/1‑94 (MQ=60)
                                             GCTGCCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGC                                                  <  SRR4302545.10451880/94‑1 (MQ=60)
                                              CTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCT                                                 >  SRR4302545.6573854/1‑94 (MQ=60)
                                              CTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCT                                                 >  SRR4302545.1302013/1‑94 (MQ=60)
                                              CTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCT                                                 >  SRR4302545.3481569/1‑94 (MQ=60)
                                               TGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTC                                                >  SRR4302545.5236064/1‑94 (MQ=60)
                                               TGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTAGACGTAGCTC                                                >  SRR4302545.9358630/1‑94 (MQ=60)
                                                GGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCT                                               <  SRR4302545.7213484/94‑1 (MQ=60)
                                                GGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCT                                               <  SRR4302545.10226508/94‑1 (MQ=60)
                                                GGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCT                                               <  SRR4302545.7213483/94‑1 (MQ=60)
                                                GGCGTTGATGCCAGCGCCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCT                                               <  SRR4302545.8868525/94‑1 (MQ=60)
                                                 GCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTG                                              >  SRR4302545.743655/1‑94 (MQ=60)
                                                   GTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCG                                            >  SRR4302545.5785449/1‑94 (MQ=60)
                                                   GTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCG                                            >  SRR4302545.3363880/1‑94 (MQ=60)
                                                   GTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCG                                            >  SRR4302545.3057718/1‑94 (MQ=60)
                                                   GTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCG                                            >  SRR4302545.1472206/1‑94 (MQ=60)
                                                   GTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCG                                            <  SRR4302545.9362151/94‑1 (MQ=60)
                                                   GTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCG                                            >  SRR4302545.931247/1‑94 (MQ=60)
                                                   GTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCG                                            >  SRR4302545.1593865/1‑94 (MQ=60)
                                                   GTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACAAGCCGTTCGACGTAGCTCTGCG                                            >  SRR4302545.8821329/1‑94 (MQ=60)
                                                   GTGGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCG                                            <  SRR4302545.145458/94‑1 (MQ=60)
                                                    TTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGTGT                                           >  SRR4302545.7261076/1‑94 (MQ=60)
                                                    TTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGT                                           <  SRR4302545.6298103/94‑1 (MQ=60)
                                                    TTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGT                                           >  SRR4302545.1881890/1‑94 (MQ=60)
                                                    TTGATGCCAGCGCCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGT                                           <  SRR4302545.9045324/94‑1 (MQ=60)
                                                     TGATGCTAGCTGCAAGCCGATTTAATCAAAGGTGAGAGGCGAATGCCGGTATTAAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTC                                          <  SRR4302545.9716190/94‑1 (MQ=60)
                                                       ATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGC                                        <  SRR4302545.628196/94‑1 (MQ=60)
                                                       ATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGC                                        >  SRR4302545.7830492/1‑94 (MQ=60)
                                                       ATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGC                                        >  SRR4302545.1757176/1‑94 (MQ=60)
                                                        TGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCT                                       >  SRR4302545.3460449/1‑94 (MQ=60)
                                                        TGCCAGCGACAACCCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCT                                       <  SRR4302545.6533837/94‑1 (MQ=60)
                                                         GCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTT                                      >  SRR4302545.962306/1‑94 (MQ=60)
                                                         GCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTT                                      <  SRR4302545.8989022/94‑1 (MQ=60)
                                                          CCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTC                                     <  SRR4302545.9858620/94‑1 (MQ=60)
                                                           CAGCGGCAAACCGAATTAATCAAAGGGGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCA                                    >  SRR4302545.4998482/1‑94 (MQ=60)
                                                            AGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAA                                   <  SRR4302545.2743886/94‑1 (MQ=60)
                                                            AGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAA                                   <  SRR4302545.2790420/94‑1 (MQ=60)
                                                            AGCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAA                                   >  SRR4302545.8323970/1‑94 (MQ=60)
                                                             GCGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAG                                  <  SRR4302545.5642281/94‑1 (MQ=60)
                                                              TGGCAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGC                                 >  SRR4302545.5355428/1‑94 (MQ=60)
                                                                GCAAACCGATTTTATCAAAGGTGAGAGGCACATGCCGGTAATTAAACCACCTGAAAACGAGCCGTTCGACGTATCTCTGCATAGCTTCAACCGT                               >  SRR4302545.7183816/1‑94 (MQ=55)
                                                                 CAACCCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTT                              <  SRR4302545.4442247/94‑1 (MQ=60)
                                                                 CAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTT                              >  SRR4302545.58164/1‑94 (MQ=60)
                                                                 CAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTT                              >  SRR4302545.1525270/1‑94 (MQ=60)
                                                                 CAAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTT                              >  SRR4302545.1525269/1‑94 (MQ=60)
                                                                  aaaccgtattatacaggGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTC                             <  SRR4302545.9691761/77‑1 (MQ=60)
                                                                  AACCCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTC                             <  SRR4302545.6918780/94‑1 (MQ=60)
                                                                  AAACGGAATTAATCAAGGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTC                             <  SRR4302545.2602786/94‑1 (MQ=60)
                                                                  AAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTC                             >  SRR4302545.8135408/1‑94 (MQ=60)
                                                                  AAACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTC                             <  SRR4302545.8047597/94‑1 (MQ=60)
                                                                   acccgaataaaaaAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCC                            <  SRR4302545.2216494/81‑1 (MQ=60)
                                                                   AACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTAC                            >  SRR4302545.5473357/1‑94 (MQ=60)
                                                                    ACCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCT                           >  SRR4302545.8299138/1‑94 (MQ=60)
                                                                     CCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTG                          >  SRR4302545.645480/1‑94 (MQ=60)
                                                                     CCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTG                          <  SRR4302545.9806145/94‑1 (MQ=60)
                                                                     CCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTG                          >  SRR4302545.5594704/1‑94 (MQ=60)
                                                                     CCGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTG                          >  SRR4302545.645479/1‑94 (MQ=60)
                                                                      CGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGC                         >  SRR4302545.2744324/1‑94 (MQ=60)
                                                                      CGAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGC                         >  SRR4302545.1828474/1‑94 (MQ=60)
                                                                       GAATTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCG                        >  SRR4302545.2765189/1‑94 (MQ=60)
                                                                       GAATAAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCG                        <  SRR4302545.4234000/94‑1 (MQ=60)
                                                                          TTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAA                     >  SRR4302545.2755765/1‑94 (MQ=60)
                                                                          TTAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAA                     >  SRR4302545.4558709/1‑94 (MQ=60)
                                                                           TAATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAA                    >  SRR4302545.2135344/1‑94 (MQ=60)
                                                                            AATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAA                   <  SRR4302545.10102121/94‑1 (MQ=60)
                                                                            AACCAAAGGTGAGAGGCGAATGCATGTAATAAAAGTACGTGAAAGCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAA                   <  SRR4302545.10164599/94‑1 (MQ=60)
                                                                             ATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAACAAG                  >  SRR4302545.5720941/1‑94 (MQ=60)
                                                                             ATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAG                  >  SRR4302545.8007198/1‑94 (MQ=60)
                                                                             ATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAG                  >  SRR4302545.2040924/1‑94 (MQ=60)
                                                                             ATCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTAGCTTCAAGCGTTCCTGCGAAAAAG                  >  SRR4302545.1388210/1‑94 (MQ=60)
                                                                              caAAAGGTGAGAGGCGCATGCCGGTAATTAAATTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGC                 <  SRR4302545.409649/92‑1 (MQ=60)
                                                                              TCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGC                 <  SRR4302545.593043/94‑1 (MQ=60)
                                                                              TCAAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGC                 >  SRR4302545.10225261/1‑94 (MQ=60)
                                                                                AAAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAG               >  SRR4302545.4372098/1‑94 (MQ=60)
                                                                                 AAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGG              >  SRR4302545.6750572/1‑94 (MQ=60)
                                                                                 AAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGG              >  SRR4302545.1149539/1‑94 (MQ=60)
                                                                                 AAGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGG              >  SRR4302545.4957835/1‑94 (MQ=60)
                                                                                 AAGGTGAAAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGG              >  SRR4302545.6380699/1‑94 (MQ=60)
                                                                                  AGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCTAGCGTTGCTGCGAAAAAGCAGGT             >  SRR4302545.7359216/1‑94 (MQ=60)
                                                                                  AGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGT             >  SRR4302545.10257892/1‑94 (MQ=60)
                                                                                  AGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGT             >  SRR4302545.10231877/1‑94 (MQ=60)
                                                                                  AGGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGT             >  SRR4302545.10527124/1‑94 (MQ=60)
                                                                                   GGTGAGAGGCGCATGCGGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTG            <  SRR4302545.1701816/94‑1 (MQ=60)
                                                                                   GGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTG            >  SRR4302545.4576093/1‑94 (MQ=60)
                                                                                   GGTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTG            >  SRR4302545.7739470/1‑94 (MQ=60)
                                                                                   GGTGAGAGGAGCAGGCCGGTAATTAAAGGACGTGAAAACGAGACGTTCGAAGTAGCTCTGCGGCGCTTCAAGCGTTCCTGCGACAAAGGAGGGG            >  SRR4302545.5731376/1‑94 (MQ=53)
                                                                                    GTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGT           >  SRR4302545.2054571/1‑94 (MQ=60)
                                                                                    GTGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGT           >  SRR4302545.242254/1‑94 (MQ=60)
                                                                                     TGAGAGGCGCATGCCGTTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTT          <  SRR4302545.1444543/94‑1 (MQ=60)
                                                                                     TGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCTTTCGACGTAGCTCTGTGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGCGTT          >  SRR4302545.2944269/1‑94 (MQ=60)
                                                                                     TGAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTT          <  SRR4302545.10764796/94‑1 (MQ=60)
                                                                                      GAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTC         <  SRR4302545.7675878/94‑1 (MQ=60)
                                                                                      GAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTC         >  SRR4302545.9527480/1‑94 (MQ=60)
                                                                                      GAGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTC         <  SRR4302545.3998736/94‑1 (MQ=60)
                                                                                       AGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT        <  SRR4302545.2380525/94‑1 (MQ=60)
                                                                                       AGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT        >  SRR4302545.1336576/1‑94 (MQ=60)
                                                                                       AGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT        <  SRR4302545.6866899/94‑1 (MQ=60)
                                                                                       AGAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT        >  SRR4302545.5812627/1‑94 (MQ=60)
                                                                                        GAGGCGCATGCCGTTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTG       <  SRR4302545.116374/94‑1 (MQ=60)
                                                                                        GAGGCGCATGCCGGTAATTAAAGTACGTGAAACCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTG       <  SRR4302545.8120159/94‑1 (MQ=60)
                                                                                        GAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGCGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTG       <  SRR4302545.5095799/94‑1 (MQ=60)
                                                                                        GAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTG       >  SRR4302545.7123526/1‑94 (MQ=60)
                                                                                        GAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTG       <  SRR4302545.3806161/94‑1 (MQ=60)
                                                                                        GAGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTG       >  SRR4302545.2145724/1‑94 (MQ=60)
                                                                                         AGGCGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGG      <  SRR4302545.9263724/94‑1 (MQ=60)
                                                                                            CGCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGG   >  SRR4302545.5618181/1‑94 (MQ=60)
                                                                                             GCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGA  >  SRR4302545.5531000/1‑94 (MQ=60)
                                                                                             |                                                                                             
AAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGA  >  NC_000913/3208327‑3208513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑