Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,221,097 2 bp→GC coding (824‑825/3093 nt) b3076 → evolved beta‑D‑galactosidase alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,221,0970CG99.8% 1458.5 / ‑5.5 420T275S (ACG→AGC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (198/221);  minor base A (0/1);  total (198/222)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01
*NC_0009133,221,0980GC100.0% 1476.6 / NA 420T275S (ACG→AGC) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (198/222);  total (198/222)

Only 100 of 434 total aligned reads displayed.

ACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCACGTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAAC  >  NC_000913/3221009‑3221185
                                                                                        ||                                                                                       
acgCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTg                                                                                     >  2:6639418/1‑94 (MQ=255)
   cTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCtt                                                                                  <  2:540162/94‑1 (MQ=255)
   cTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCtt                                                                                  >  2:6378275/1‑94 (MQ=255)
       aaTATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTAc                                                                              <  1:8102255/94‑1 (MQ=255)
       aaTATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTAc                                                                              >  1:1343700/1‑94 (MQ=255)
         tataCCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTg                                                                            <  1:9053261/94‑1 (MQ=255)
          ataCCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGt                                                                           <  1:4551427/94‑1 (MQ=255)
           taCCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTc                                                                          >  1:6789279/1‑94 (MQ=255)
            tcccTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCg                                                                         <  1:5579501/93‑1 (MQ=255)
            aCCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCg                                                                         >  2:3424865/1‑94 (MQ=255)
              ccTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGaa                                                                       <  1:7008572/94‑1 (MQ=255)
               cTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAAc                                                                      >  2:453734/1‑94 (MQ=255)
                tGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACa                                                                     >  2:2493971/1‑94 (MQ=255)
                tGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACa                                                                     >  1:4439911/1‑94 (MQ=255)
                  tttGATGGCGAACGCGTGGTGGACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGc                                                                   >  2:5082338/1‑94 (MQ=255)
                   ttGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGcc                                                                  <  2:6967406/94‑1 (MQ=255)
                   ttGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGcc                                                                  <  2:2705845/94‑1 (MQ=255)
                    tGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCg                                                                 >  1:3581857/1‑94 (MQ=255)
                    tGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCg                                                                 >  1:7540177/1‑94 (MQ=255)
                    tGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCg                                                                 <  1:10571737/94‑1 (MQ=255)
                     gATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCgc                                                                >  1:371373/1‑94 (MQ=255)
                     gATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCgc                                                                <  1:10093434/94‑1 (MQ=255)
                      aTGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCgca                                                               >  1:244858/1‑94 (MQ=255)
                      aTGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCgca                                                               >  2:5902531/1‑94 (MQ=255)
                       tGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCgcag                                                              <  2:8289913/94‑1 (MQ=255)
                        ggCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCgcagc                                                             <  1:3185186/94‑1 (MQ=255)
                          cGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCaa                                                           <  2:2390836/94‑1 (MQ=255)
                          cGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCaa                                                           >  2:3819428/1‑94 (MQ=255)
                          cGAACGCGTGGTGCACAGCAGCGCCATTGACCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCaa                                                           >  1:2811662/1‑94 (MQ=255)
                           gAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCTCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAAt                                                          >  1:1480154/1‑94 (MQ=255)
                           gAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAAt                                                          >  2:6440683/1‑94 (MQ=255)
                            aaCGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATg                                                         <  1:10279215/94‑1 (MQ=255)
                              cgcgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGt                                                       <  2:3512/94‑1 (MQ=255)
                               gcgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTc                                                      >  2:958822/1‑94 (MQ=255)
                               gcgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTc                                                      >  1:5980040/1‑94 (MQ=255)
                               gcgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTc                                                      >  2:8466578/1‑94 (MQ=255)
                               gcgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTc                                                      <  1:2063071/94‑1 (MQ=255)
                                cgTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTca                                                     <  1:7385081/94‑1 (MQ=255)
                                 gtggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcag                                                    <  2:7767197/94‑1 (MQ=255)
                                  tggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcagc                                                   <  1:873426/94‑1 (MQ=255)
                                  tggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcagc                                                   <  1:1561354/94‑1 (MQ=255)
                                   ggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGCACAGACGCAGAAATGGAcagag                                                  >  2:7644163/1‑92 (MQ=255)
                                   ggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcagca                                                  >  2:6120245/1‑94 (MQ=255)
                                   ggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcagca                                                  <  1:8244323/94‑1 (MQ=255)
                                   ggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTcagca                                                  <  1:1820019/94‑1 (MQ=255)
                                      gCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGaa                                               <  1:936220/94‑1 (MQ=255)
                                       cacaGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAAt                                              >  1:7301075/1‑94 (MQ=255)
                                       cacaGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAAt                                              >  1:6907410/1‑94 (MQ=255)
                                        acaGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATc                                             >  2:4828877/1‑94 (MQ=255)
                                         cagcagCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATcc                                            >  2:8996956/1‑94 (MQ=255)
                                         cagcagCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATcc                                            <  2:10022725/94‑1 (MQ=255)
                                          agcagcGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATccc                                           <  2:7918122/94‑1 (MQ=255)
                                            cagcGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCt                                         <  1:9541859/94‑1 (MQ=255)
                                             agcGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCtt                                        >  1:6447846/1‑94 (MQ=255)
                                             agcGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCtt                                        >  1:7593165/1‑94 (MQ=255)
                                             agcGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCtt                                        >  1:902165/1‑94 (MQ=255)
                                              gcgcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTa                                       <  1:3092072/94‑1 (MQ=255)
                                              gcgcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTa                                       <  2:125297/94‑1 (MQ=255)
                                              gcgcCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTa                                       >  2:4300548/1‑94 (MQ=255)
                                                 ccATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCt                                    <  2:1511866/94‑1 (MQ=255)
                                                  cATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCtt                                   >  2:8842646/1‑94 (MQ=255)
                                                     tGAACATTTGGCAATTGAAAAACCTGCCCGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAc                                <  1:10164276/94‑1 (MQ=255)
                                                      gATCATTTGGCAATTGAAAAACTGACCAGGGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTAcc                               <  2:10164228/94‑1 (MQ=255)
                                                        tCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCAt                             <  2:1976848/94‑1 (MQ=255)
                                                          aTTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCt                           >  1:2310592/1‑94 (MQ=255)
                                                          aTTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAACCCCCTTATCTTTACCATCt                           <  2:1817489/94‑1 (MQ=255)
                                                           tttGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTg                          <  1:384239/94‑1 (MQ=255)
                                                            tttgCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTgg                         <  1:6583660/91‑1 (MQ=255)
                                                             tGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGt                        <  2:10348277/94‑1 (MQ=255)
                                                             tGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGt                        >  1:4873930/1‑94 (MQ=255)
                                                              ggCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTc                       <  2:7245083/94‑1 (MQ=255)
                                                               gCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCa                      <  2:2930804/94‑1 (MQ=255)
                                                               gCAATTGAAAAACTGACCAGAGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCa                      <  1:10395180/94‑1 (MQ=255)
                                                                cAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCAt                     >  2:10438267/1‑94 (MQ=255)
                                                                  aTTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGa                   <  1:98879/94‑1 (MQ=255)
                                                                  aTTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGa                   <  1:1254261/94‑1 (MQ=255)
                                                                  aTTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGa                   <  1:5748409/94‑1 (MQ=255)
                                                                  aTTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGa                   >  2:1438266/1‑94 (MQ=255)
                                                                      aaaaaCTGACCAGCGCCATCTTTGCTTTCACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCt               <  2:6317784/94‑1 (MQ=255)
                                                                       aaaaCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTg              <  2:2216634/94‑1 (MQ=255)
                                                                       aaaaCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTg              <  2:1094506/94‑1 (MQ=255)
                                                                        aaaCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGa             <  2:5625220/94‑1 (MQ=255)
                                                                        aaaCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGa             >  1:7882981/1‑94 (MQ=255)
                                                                         aaCTGCCCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGaa            <  1:5425036/94‑1 (MQ=255)
                                                                          aCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGaaa           <  2:4187834/94‑1 (MQ=255)
                                                                           cTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCGGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAg          >  1:9186093/1‑94 (MQ=255)
                                                                           cTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAg          >  1:713182/1‑94 (MQ=255)
                                                                           cTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAg          >  2:6854180/1‑94 (MQ=255)
                                                                           cTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAg          >  2:8170869/1‑94 (MQ=255)
                                                                           cTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAg          >  1:6257384/1‑94 (MQ=255)
                                                                            tGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGa         >  2:4002826/1‑94 (MQ=255)
                                                                             gACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGAc        <  1:2230416/94‑1 (MQ=255)
                                                                              aCCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACg       <  2:5305201/94‑1 (MQ=255)
                                                                              aCCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACg       >  1:5375468/1‑94 (MQ=255)
                                                                               ccAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGc      >  1:2744820/1‑94 (MQ=255)
                                                                               ccAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGc      <  2:9162724/94‑1 (MQ=255)
                                                                                cAGCGCCAGCTTTGCTTTTACTGTCGTACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGTTCATGACGCTGAAAGACGcc     >  2:9392339/1‑94 (MQ=255)
                                                                                  gcgcCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCaa   <  1:3456995/94‑1 (MQ=255)
                                                                                  gcgcCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCaa   <  2:253351/94‑1 (MQ=255)
                                                                                   cgcCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAAc  <  1:8477570/94‑1 (MQ=255)
                                                                                        ||                                                                                       
ACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCACGTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAAC  >  NC_000913/3221009‑3221185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGC‑‑CAAG‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACT  >  NC_000913/3222851‑3223027
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AACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGC‑‑A                                                                                         <  SRR4302545.1166008/94‑1 (MQ=60)
             ATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGC                                                                        >  SRR4302545.157187/1‑94 (MQ=60)
              TCAGGTGGCGTATTACGGTCGTGGACCGTGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGC‑‑‑‑AGCTC‑‑CGTCGATGCC                                                                       <  SRR4302545.4721495/94‑1 (MQ=60)
              TCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCC                                                                       >  SRR4302545.4090951/1‑94 (MQ=60)
               CAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTCGCGC‑‑‑‑AG‑‑CAGCGTCGATGCCA                                                                      >  SRR4302545.1003822/1‑94 (MQ=60)
                AGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCAT                                                                     <  SRR4302545.1970161/94‑1 (MQ=60)
                 GGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATG                                                                    >  SRR4302545.6406008/1‑94 (MQ=60)
                 GGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATG                                                                    >  SRR4302545.10148138/1‑94 (MQ=60)
                 GGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATG                                                                    >  SRR4302545.4107989/1‑94 (MQ=60)
                  GTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGT                                                                   <  SRR4302545.4073313/94‑1 (MQ=60)
                  GTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGT                                                                   <  SRR4302545.5305658/94‑1 (MQ=60)
                   TGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTT                                                                  >  SRR4302545.434966/1‑94 (MQ=60)
                    GGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTC                                                                 <  SRR4302545.7766084/94‑1 (MQ=60)
                    GGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTC                                                                 >  SRR4302545.2009536/1‑94 (MQ=60)
                    GGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTC                                                                 <  SRR4302545.8382126/94‑1 (MQ=60)
                    GGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTC                                                                 <  SRR4302545.10741794/94‑1 (MQ=60)
                     GCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCG                                                                <  SRR4302545.9589596/94‑1 (MQ=60)
                     GCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCG                                                                >  SRR4302545.7236975/1‑94 (MQ=60)
                       GTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAG                                                              <  SRR4302545.10629455/94‑1 (MQ=60)
                       GTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAG                                                              >  SRR4302545.7898824/1‑94 (MQ=60)
                       GTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAG                                                              <  SRR4302545.4159543/94‑1 (MQ=60)
                       GTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAG                                                              <  SRR4302545.3400557/94‑1 (MQ=60)
                       GTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAG                                                              <  SRR4302545.10405841/94‑1 (MQ=60)
                       GTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAG                                                              >  SRR4302545.4615691/1‑94 (MQ=60)
                       GTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGGTCGAG                                                              >  SRR4302545.5564140/1‑94 (MQ=60)
                        TATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGA                                                             >  SRR4302545.2025511/1‑94 (MQ=60)
                        TATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGCTCGAGA                                                             >  SRR4302545.1820366/1‑94 (MQ=60)
                         ATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAA                                                            >  SRR4302545.6434769/1‑94 (MQ=60)
                         ATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAA                                                            >  SRR4302545.6434767/1‑94 (MQ=60)
                         ATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAA                                                            >  SRR4302545.321535/1‑94 (MQ=60)
                         ATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAA                                                            <  SRR4302545.7068395/94‑1 (MQ=60)
                          TTGCGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAAC                                                           <  SRR4302545.9026764/94‑1 (MQ=60)
                          TTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAAC                                                           >  SRR4302545.527360/1‑94 (MQ=60)
                           TACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACT                                                          <  SRR4302545.6113553/94‑1 (MQ=60)
                           TACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACT                                                          <  SRR4302545.2057405/94‑1 (MQ=60)
                           TACAGTCGTGGACCGGGCGAAAACTCCGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGCCGATGCCATGTTCGAGGACT                                                          >  SRR4302545.7249642/1‑94 (MQ=60)
                            ACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTA                                                         <  SRR4302545.1994054/94‑1 (MQ=60)
                             CGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCCTCGCTATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGCACTCT                                                        >  SRR4302545.4049987/1‑94 (MQ=60)
                             CGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTAT                                                        <  SRR4302545.463525/94‑1 (MQ=60)
                             CGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTAT                                                        <  SRR4302545.2251095/94‑1 (MQ=60)
                             CGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTAT                                                        >  SRR4302545.1204428/1‑94 (MQ=60)
                             CGGTCGTGAGCCGAGCGAAAACTATGACGACAGACAGTAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTAT                                                        <  SRR4302545.294123/94‑1 (MQ=38)
                              GGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATCCCATGTTCGAGAACTATC                                                       <  SRR4302545.4854821/94‑1 (MQ=60)
                               GTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCC                                                      >  SRR4302545.3072534/1‑94 (MQ=60)
                               GTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCC                                                      <  SRR4302545.2522386/94‑1 (MQ=60)
                               GTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCC                                                      <  SRR4302545.9852646/94‑1 (MQ=60)
                               GTCGTGGACAGGGCGAAACCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCC                                                      <  SRR4302545.10884767/94‑1 (MQ=60)
                                TCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCC                                                     >  SRR4302545.9371919/1‑94 (MQ=60)
                                 CGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCTCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCT                                                    >  SRR4302545.6867085/1‑94 (MQ=60)
                                 CGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGCACTATCCCT                                                    >  SRR4302545.6867086/1‑94 (MQ=60)
                                 CGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCT                                                    <  SRR4302545.1704741/94‑1 (MQ=60)
                                 CGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCT                                                    <  SRR4302545.3188834/94‑1 (MQ=60)
                                 CGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGACGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCT                                                    <  SRR4302545.8806387/94‑1 (MQ=60)
                                 CGTGCACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCT                                                    <  SRR4302545.10113412/94‑1 (MQ=60)
                                  GTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTT                                                   >  SRR4302545.5407595/1‑94 (MQ=60)
                                  GTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTT                                                   >  SRR4302545.785233/1‑94 (MQ=60)
                                   TGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTC                                                  <  SRR4302545.4962258/94‑1 (MQ=60)
                                   TGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTC                                                  >  SRR4302545.1706574/1‑94 (MQ=60)
                                    GGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGATCTATACCTTCC                                                 >  SRR4302545.4172391/1‑94 (MQ=60)
                                    GGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCC                                                 <  SRR4302545.1528461/94‑1 (MQ=60)
                                    GCACCGGGCGAAAACGGCGCAGACAGCCAGCAGGCTAACGTCACCGATATCTGGCGC‑‑‑‑AGCCC‑‑CGTCGATACCATGTTCGAGAACTATCCCTTCC                                                 <  SRR4302545.8152128/94‑1 (MQ=42)
                                     GACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCC                                                >  SRR4302545.3145206/1‑94 (MQ=60)
                                      ACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCG                                               >  SRR4302545.9614374/1‑94 (MQ=60)
                                        CGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCA                                             <  SRR4302545.724054/94‑1 (MQ=60)
                                         GGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCTCAG                                            <  SRR4302545.3374280/94‑1 (MQ=60)
                                         GGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAG                                            >  SRR4302545.7371621/1‑94 (MQ=60)
                                         GGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAG                                            >  SRR4302545.1410309/1‑94 (MQ=60)
                                          GGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGA                                           >  SRR4302545.7332645/1‑94 (MQ=60)
                                           GCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAA                                          <  SRR4302545.6279704/94‑1 (MQ=60)
                                           GCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGC‑‑AGCG‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAA                                          <  SRR4302545.1789870/94‑1 (MQ=60)
                                            CGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAAC                                         >  SRR4302545.9906311/1‑94 (MQ=60)
                                            CGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAAC                                         <  SRR4302545.4821176/94‑1 (MQ=60)
                                            CGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAAC                                         >  SRR4302545.1102970/1‑94 (MQ=60)
                                             GAAAACTACGCCGACAGCCAGCAGGCTAAGATCATCGATATCTAGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGGTCGAGAACTATCTCTTCCCGCAGAACA                                        >  SRR4302545.10172266/1‑94 (MQ=60)
                                             GAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACA                                        <  SRR4302545.2055247/94‑1 (MQ=60)
                                              AAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAA                                       >  SRR4302545.10254107/1‑94 (MQ=60)
                                              AAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAA                                       >  SRR4302545.4101703/1‑94 (MQ=60)
                                               AACCTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAAC                                      <  SRR4302545.6671588/94‑1 (MQ=60)
                                               AAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAAC                                      >  SRR4302545.6537267/1‑94 (MQ=60)
                                               AAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAAC                                      >  SRR4302545.4477598/1‑94 (MQ=60)
                                                AACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACG                                     >  SRR4302545.3597524/1‑94 (MQ=60)
                                                AACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACG                                     >  SRR4302545.3620555/1‑94 (MQ=60)
                                                AACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACG                                     >  SRR4302545.4700825/1‑94 (MQ=60)
                                                AACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACG                                     >  SRR4302545.10354977/1‑94 (MQ=60)
                                                AACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACG                                     <  SRR4302545.3052192/94‑1 (MQ=60)
                                                 ACTACCCCGACAGCCAGCAGGCTAACATCATCCATATCTGGCCCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGG                                    <  SRR4302545.4719402/94‑1 (MQ=60)
                                                  CTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGT                                   >  SRR4302545.3309232/1‑94 (MQ=60)
                                                  CTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTAGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGT                                   >  SRR4302545.533107/1‑94 (MQ=60)
                                                   TACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTA                                  <  SRR4302545.912709/94‑1 (MQ=60)
                                                   TACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTA                                  >  SRR4302545.4575930/1‑94 (MQ=60)
                                                     CGCCGACAGCCAGCAGGCTCACATCATCGATATCTGGCGC‑‑‑‑AGCTC‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAC                                <  SRR4302545.9676778/94‑1 (MQ=60)
                                                     CGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAC                                <  SRR4302545.1644804/94‑1 (MQ=60)
                                                     CGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAC                                >  SRR4302545.9181477/1‑94 (MQ=60)
                                                     CGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAC                                >  SRR4302545.6858535/1‑94 (MQ=60)
                                                      GCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACC                               <  SRR4302545.1348608/94‑1 (MQ=60)
                                                      GCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACC                               >  SRR4302545.5376775/1‑94 (MQ=60)
                                                      GCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACC                               <  SRR4302545.9174418/94‑1 (MQ=60)
                                                      GCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACC                               <  SRR4302545.2412214/94‑1 (MQ=60)
                                                      GCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACC                               >  SRR4302545.8493891/1‑94 (MQ=60)
                                                      GCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACC                               >  SRR4302545.3161978/1‑94 (MQ=60)
                                                      GCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACC                               <  SRR4302545.8029098/94‑1 (MQ=60)
                                                      GCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGGAACC                               >  SRR4302545.2114896/1‑94 (MQ=60)
                                                       CCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCG                              <  SRR4302545.10355530/94‑1 (MQ=60)
                                                       CCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCG                              <  SRR4302545.9219681/94‑1 (MQ=60)
                                                       CCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCG                              <  SRR4302545.7685290/94‑1 (MQ=60)
                                                       CAGACAGCCAGCAGCCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTCCCCGCAGAACAACGGTAACCG                              <  SRR4302545.5461802/94‑1 (MQ=60)
                                                        CGACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGT                             <  SRR4302545.10660905/94‑1 (MQ=60)
                                                         GACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTC                            <  SRR4302545.6738969/94‑1 (MQ=60)
                                                         GACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTC                            >  SRR4302545.3018955/1‑94 (MQ=60)
                                                         GACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAAAAACGGTAACCGTC                            >  SRR4302545.9457061/1‑94 (MQ=60)
                                                         GACAGCCAGCAGGCTAACATCATCGATATCTGGCGC‑‑AGAA‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTC                            <  SRR4302545.9684498/94‑1 (MQ=60)
                                                          ACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCA                           <  SRR4302545.5779006/94‑1 (MQ=60)
                                                          ACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCA                           <  SRR4302545.3462712/94‑1 (MQ=60)
                                                          ACAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCA                           >  SRR4302545.4782410/1‑94 (MQ=60)
                                                           CAGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAG                          <  SRR4302545.8271560/94‑1 (MQ=60)
                                                            AGCCAGCAGGCTAGCATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGC                         >  SRR4302545.2911156/1‑94 (MQ=60)
                                                            AGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGC                         >  SRR4302545.1795409/1‑94 (MQ=60)
                                                            AGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGC                         >  SRR4302545.7708301/1‑94 (MQ=60)
                                                            AGCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATCCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGC                         <  SRR4302545.5888177/94‑1 (MQ=60)
                                                             aacaagagtCTAACATCATCGATATCTGCCGC‑‑AACA‑‑C‑‑CGTCGATGCCATGTACGAGCACAACCCCTTCCCGCAGAACAACGGTATCCGGCAGCA                        <  SRR4302545.1775573/85‑1 (MQ=27)
                                                             GCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCA                        >  SRR4302545.6978056/1‑94 (MQ=60)
                                                             GCCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCA                        >  SRR4302545.4661518/1‑94 (MQ=60)
                                                              CCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCAT                       <  SRR4302545.4289106/94‑1 (MQ=60)
                                                              CCAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCAT                       <  SRR4302545.1682670/94‑1 (MQ=60)
                                                              CCAGCAGGCTAACATCATCCATATCTCGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGATCGAGAACTATCCCTTCCCGCACAACAACGGTAACCGTCAGCAT                       >  SRR4302545.10397875/1‑94 (MQ=60)
                                                               CAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATG                      >  SRR4302545.6743764/1‑94 (MQ=60)
                                                               CAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATG                      >  SRR4302545.4258578/1‑94 (MQ=60)
                                                               CAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATG                      <  SRR4302545.6650671/94‑1 (MQ=60)
                                                               CAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATG                      <  SRR4302545.73719/94‑1 (MQ=60)
                                                               CAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATG                      >  SRR4302545.9604923/1‑94 (MQ=60)
                                                               CAGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATG                      >  SRR4302545.4914248/1‑94 (MQ=60)
                                                                AGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGT                     <  SRR4302545.10920146/94‑1 (MQ=60)
                                                                AGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGT                     <  SRR4302545.2013351/94‑1 (MQ=60)
                                                                AGCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGT                     >  SRR4302545.9859241/1‑94 (MQ=60)
                                                                 GCAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTC                    <  SRR4302545.3743131/94‑1 (MQ=60)
                                                                  CAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCC                   >  SRR4302545.8131771/1‑94 (MQ=60)
                                                                  CAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCC                   >  SRR4302545.10920556/1‑94 (MQ=60)
                                                                  CAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCC                   <  SRR4302545.968009/94‑1 (MQ=60)
                                                                  CAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCC                   <  SRR4302545.10902111/94‑1 (MQ=60)
                                                                  CAGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCC                   >  SRR4302545.6061219/1‑94 (MQ=60)
                                                                   AGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCG                  >  SRR4302545.10072938/1‑94 (MQ=60)
                                                                   AGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCG                  >  SRR4302545.2765808/1‑94 (MQ=60)
                                                                   AGGCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCG                  >  SRR4302545.6599623/1‑94 (MQ=60)
                                                                     GCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCT                >  SRR4302545.7023251/1‑94 (MQ=60)
                                                                     GCTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCT                <  SRR4302545.9016501/94‑1 (MQ=60)
                                                                      CTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCTATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTA               <  SRR4302545.7660268/94‑1 (MQ=60)
                                                                      CTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTG               <  SRR4302545.6463249/94‑1 (MQ=60)
                                                                      CTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTG               <  SRR4302545.8176312/94‑1 (MQ=60)
                                                                      CTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTG               <  SRR4302545.6463250/94‑1 (MQ=60)
                                                                      CTAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTG               <  SRR4302545.2088949/94‑1 (MQ=60)
                                                                       TAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGG              <  SRR4302545.2350703/94‑1 (MQ=60)
                                                                       TAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGG              >  SRR4302545.1285384/1‑94 (MQ=60)
                                                                       TAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGG              <  SRR4302545.3670446/94‑1 (MQ=60)
                                                                       TAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGG              >  SRR4302545.7974908/1‑94 (MQ=60)
                                                                       TAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGG              <  SRR4302545.4228937/94‑1 (MQ=60)
                                                                       TAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGG              >  SRR4302545.10667258/1‑94 (MQ=60)
                                                                       TAACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGG              <  SRR4302545.7395852/94‑1 (MQ=60)
                                                                        AACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGA             >  SRR4302545.10870989/1‑94 (MQ=60)
                                                                        AACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGA             <  SRR4302545.10351803/94‑1 (MQ=60)
                                                                         ACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGAC            >  SRR4302545.8383304/1‑94 (MQ=60)
                                                                         ACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGAC            <  SRR4302545.8773783/94‑1 (MQ=60)
                                                                         ACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGAC            >  SRR4302545.8383305/1‑94 (MQ=60)
                                                                         ACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGAC            <  SRR4302545.10247782/94‑1 (MQ=60)
                                                                         ACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGAC            <  SRR4302545.3599214/94‑1 (MQ=60)
                                                                         ACATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGAC            <  SRR4302545.4916066/94‑1 (MQ=60)
                                                                          CATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACG           <  SRR4302545.1223222/94‑1 (MQ=60)
                                                                          CATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACG           <  SRR4302545.9969313/94‑1 (MQ=60)
                                                                           ATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGG          <  SRR4302545.2308062/94‑1 (MQ=60)
                                                                           ATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGG          <  SRR4302545.569681/94‑1 (MQ=60)
                                                                           ATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGG          >  SRR4302545.1911347/1‑94 (MQ=60)
                                                                           ATCATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGG          >  SRR4302545.3047622/1‑94 (MQ=60)
                                                                             CATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAAGCGTCAGCATGTCCTCTGGACAGCA        >  SRR4302545.3028462/1‑94 (MQ=60)
                                                                             CATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCA        <  SRR4302545.10519243/94‑1 (MQ=60)
                                                                              ATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCAC       >  SRR4302545.634467/1‑94 (MQ=60)
                                                                              ATCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCAC       >  SRR4302545.5231923/1‑94 (MQ=60)
                                                                               TCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACT      <  SRR4302545.6922239/94‑1 (MQ=60)
                                                                               TCGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACT      <  SRR4302545.10427722/94‑1 (MQ=60)
                                                                                CGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTG     <  SRR4302545.9486405/94‑1 (MQ=60)
                                                                                CGATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTG     >  SRR4302545.8725710/1‑94 (MQ=60)
                                                                                 GATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGA    >  SRR4302545.10862876/1‑94 (MQ=60)
                                                                                  ATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGGACTGAC   >  SRR4302545.8173145/1‑94 (MQ=60)
                                                                                  ATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGAC   <  SRR4302545.4587232/94‑1 (MQ=60)
                                                                                  ATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGAC   >  SRR4302545.8173144/1‑94 (MQ=60)
                                                                                  ATATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTCAC   >  SRR4302545.1630302/1‑94 (MQ=60)
                                                                                   TATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACT  <  SRR4302545.10559258/94‑1 (MQ=60)
                                                                                   TATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACT  <  SRR4302545.10559257/94‑1 (MQ=60)
                                                                                   TATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACT  <  SRR4302545.3754662/94‑1 (MQ=60)
                                                                                   TATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACT  <  SRR4302545.10221748/94‑1 (MQ=60)
                                                                                   TATCTGGCGCAGCA‑‑‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACT  <  SRR4302545.2942055/94‑1 (MQ=60)
                                                                                               |                                                                                       
AACGGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGCCAGCAGGCTAACATCATCGATATCTGGCGC‑‑CAAG‑‑C‑‑CGTCGATGCCATGTTCGAGAACTATCCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACT  >  NC_000913/3222851‑3223027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑