Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,224,879 Δ1 bp coding (1005/1434 nt) ygjI → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,224,8780G.99.8% 1935.4 / ‑3.7 425coding (1004/1434 nt)ygjIputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base . (233/191);  minor base A (1/0);  total (234/191)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 489 total aligned reads displayed.

CCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACT  >  NC_000913/3224785‑3224966
                                                                                             |                                                                                         
ccGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCcggcgcg                                                                                           <  1:9216358/94‑1 (MQ=255)
ccGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCcggcgcg                                                                                           >  1:6877582/1‑94 (MQ=255)
 cGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCcggcgcgc                                                                                          >  1:9790792/1‑93 (MQ=255)
  tcAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCcggcgcgcg                                                                                         >  1:4079166/2‑92 (MQ=255)
  gCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCcggcgcgcg                                                                                         <  2:567354/94‑3 (MQ=255)
  gCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTCCcggcgcgcg                                                                                         <  1:1568218/94‑3 (MQ=255)
   cAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCcggcgcgcgc                                                                                        >  2:9215960/1‑91 (MQ=255)
   cAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCcggcgcgcgc                                                                                        <  2:323513/94‑4 (MQ=255)
   cAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCcggcgcgcga                                                                                        >  2:7591744/1‑91 (MQ=255)
    aaCGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCcggcgcgcgca                                                                                       >  1:1195574/1‑90 (MQ=255)
    aaCGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCcggcgcgcgca                                                                                       >  1:1387432/1‑90 (MQ=255)
    aaCGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCcggcgcgcgca                                                                                       <  2:3701504/94‑5 (MQ=255)
      cGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGc                                                                                    <  2:2005731/94‑1 (MQ=255)
       gCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCg                                                                                   >  1:2910221/1‑94 (MQ=255)
         cGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTg                                                                                 <  2:785024/94‑1 (MQ=255)
         cGGTGAAAATTTTCTTCTCCGAAATACCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGAGC‑GCGCAGCGTg                                                                                 >  2:3844344/1‑94 (MQ=255)
           gTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGa                                                                               >  2:4007147/1‑94 (MQ=255)
           gTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGa                                                                               <  2:7326255/94‑1 (MQ=255)
            tGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGAGGac                                                                              >  1:8041222/1‑93 (MQ=255)
             gAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATc                                                                             >  1:8353569/1‑94 (MQ=255)
              aaaaTTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATcc                                                                            >  2:1091243/1‑94 (MQ=255)
               aaaTTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCa                                                                           >  2:3940470/1‑94 (MQ=255)
                aaTTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAg                                                                          >  2:6141652/1‑94 (MQ=255)
                  ttttCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGtt                                                                        >  2:5501663/1‑94 (MQ=255)
                   cttcttcTCCAAAATCCCGCAAGGCATCTTTCGTAAGAAAACCGTCGAACTGAACGAAATCGGCGTGCCGGCGC‑GCGCAGCGTGGATCCAGTTc                                                                       <  1:5291821/93‑1 (MQ=255)
                    ttcttcTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTcc                                                                      >  1:1686018/1‑94 (MQ=255)
                     tcttctCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCt                                                                     >  1:8491582/1‑94 (MQ=255)
                     tcttctCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCt                                                                     <  2:2999758/94‑1 (MQ=255)
                     tcttctCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCt                                                                     >  1:2467009/1‑94 (MQ=255)
                      cttctCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTg                                                                    <  2:951983/94‑1 (MQ=255)
                      cttctCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTg                                                                    <  1:4744750/94‑1 (MQ=255)
                        tctcCGAAATCCCAGAAGGCATCTTTGGGAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGAt                                                                  <  1:9131687/94‑1 (MQ=255)
                         ctcCGAAATCCCGGAGGCCATCTGTGGTAAGAACACCGTCGAACTGAACGAAATCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATc                                                                 <  2:6229227/94‑1 (MQ=255)
                           ccGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGt                                                               <  2:4530617/94‑1 (MQ=255)
                           ccGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGt                                                               <  2:2866416/94‑1 (MQ=255)
                            cGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTc                                                              >  2:2715175/1‑94 (MQ=255)
                             gAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTct                                                             >  1:7780991/1‑93 (MQ=255)
                             gAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCa                                                             >  2:672946/1‑94 (MQ=255)
                             gAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCa                                                             >  1:7345259/1‑94 (MQ=255)
                                aTCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATcc                                                          <  1:875595/94‑1 (MQ=255)
                                 tCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATccc                                                         <  2:515656/94‑1 (MQ=255)
                                   ccGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGc                                                       >  1:3131890/1‑94 (MQ=255)
                                    cGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCt                                                      <  2:219093/94‑1 (MQ=255)
                                    cGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCt                                                      >  2:8770959/1‑94 (MQ=255)
                                     ggAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCtg                                                     <  1:3678799/94‑1 (MQ=255)
                                      gAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCtgc                                                    >  1:5146803/1‑93 (MQ=255)
                                       aaGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCtgat                                                   >  1:10140076/1‑94 (MQ=255)
                                         ggCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCtgatgt                                                 >  1:7896760/1‑93 (MQ=255)
                                         ggCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCtgatga                                                 >  2:1156133/1‑94 (MQ=255)
                                           cATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGAtt                                               >  2:3419659/1‑94 (MQ=255)
                                            aTCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTa                                              <  1:2164253/94‑1 (MQ=255)
                                            aTCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTa                                              >  1:2648389/1‑94 (MQ=255)
                                              cTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑c                                           >  2:9783589/1‑94 (MQ=255)
                                              cTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑c                                           >  1:5526485/1‑94 (MQ=255)
                                               tttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cc                                          <  2:10019919/94‑1 (MQ=255)
                                                ttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑ccc                                         >  1:3251062/1‑93 (MQ=255)
                                                   gTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cccgat                                      >  1:5982463/1‑90 (MQ=255)
                                                   gTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cccgat                                      <  1:6127526/94‑5 (MQ=255)
                                                     aaGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGc                                     >  1:7424935/1‑94 (MQ=255)
                                                     aaGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGc                                     >  1:6593595/1‑94 (MQ=255)
                                                      aGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCt                                    >  1:2317378/1‑94 (MQ=255)
                                                       gAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTc                                   >  1:9530840/1‑94 (MQ=255)
                                                       gAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGAGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTc                                   <  1:5783780/94‑1 (MQ=255)
                                                        aaaaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCg                                  >  2:5288897/1‑94 (MQ=255)
                                                          aaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGt                                >  1:4322157/1‑94 (MQ=255)
                                                          aaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGt                                >  1:9923158/1‑94 (MQ=255)
                                                           aCCGTCGAACTGAACTAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCTGtt                               <  1:8178834/94‑1 (MQ=255)
                                                           aCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCAGCTGATGATTATCCCGATGCTCGGtt                               <  2:1676634/94‑1 (MQ=255)
                                                            ccGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTc                              <  1:10057244/94‑1 (MQ=255)
                                                             cGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTcc                             >  1:8916383/1‑94 (MQ=255)
                                                               tCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCaa                           >  1:6284293/1‑94 (MQ=255)
                                                               tCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCaa                           >  1:1068337/1‑94 (MQ=255)
                                                                cGAGCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAAt                          <  1:717715/94‑1 (MQ=255)
                                                                cGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAAt                          <  2:10370302/94‑1 (MQ=255)
                                                                cGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAAt                          >  2:8559212/1‑94 (MQ=255)
                                                                 gAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATa                         <  2:6837854/94‑1 (MQ=255)
                                                                   aCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAcc                       <  2:4738423/94‑1 (MQ=255)
                                                                   aCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAcc                       <  2:1366078/94‑1 (MQ=255)
                                                                    cTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCg                      <  1:3075539/94‑1 (MQ=255)
                                                                    cTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCg                      >  2:7128428/1‑94 (MQ=255)
                                                                     tGAACGCAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGCTCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGt                     <  1:9401486/94‑1 (MQ=255)
                                                                        aCGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCa                  >  1:3420870/1‑94 (MQ=255)
                                                                        aCGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCa                  >  2:6821191/1‑94 (MQ=255)
                                                                         cGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAg                 >  2:9291849/1‑94 (MQ=255)
                                                                           aaaaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGa               >  2:8955275/1‑94 (MQ=255)
                                                                           aaaaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGa               <  2:2514441/94‑1 (MQ=255)
                                                                            aaccGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGAt              <  1:396782/91‑1 (MQ=255)
                                                                            aaaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGAt              <  2:4272627/94‑1 (MQ=255)
                                                                            aaaCGGCGTCCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGAt              <  2:10215197/94‑1 (MQ=255)
                                                                             aaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATc             <  1:7644479/94‑1 (MQ=255)
                                                                             aaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATc             >  2:4607121/1‑94 (MQ=255)
                                                                             aaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATc             <  2:6466461/94‑1 (MQ=255)
                                                                               cGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtg           >  2:8626987/1‑94 (MQ=255)
                                                                               cGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtg           <  1:1833920/94‑1 (MQ=255)
                                                                               cGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtg           >  2:2313296/1‑94 (MQ=255)
                                                                                   gTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTTATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatga       >  1:37875/1‑94 (MQ=255)
                                                                                   gTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatga       >  2:8363096/1‑94 (MQ=255)
                                                                                   gTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatga       >  2:5386254/1‑94 (MQ=255)
                                                                                     tCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAAt     >  1:10523677/1‑94 (MQ=255)
                                                                                      ccGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATa    <  2:1545493/94‑1 (MQ=255)
                                                                                             |                                                                                         
CCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACT  >  NC_000913/3224785‑3224966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 284 total aligned reads displayed.
TTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑C‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCT  >  NC_000913/3224832‑3225019
                                                                                                |                                                                                             
TTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑C‑C                                                                                               <  SRR4302545.1570918/94‑1 (MQ=60)
TTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑C‑C                                                                                               <  SRR4302545.9480008/94‑1 (MQ=60)
 TTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑C‑CC                                                                                              <  SRR4302545.2391716/94‑1 (MQ=60)
  TGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑C‑Ccg                                                                                             <  SRR4302545.9754759/94‑3 (MQ=60)
  TGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑C‑Ccg                                                                                             <  SRR4302545.1689580/94‑3 (MQ=60)
   GGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑C‑Ccga                                                                                            >  SRR4302545.6424163/1‑91 (MQ=60)
   GGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑C‑Ccga                                                                                            >  SRR4302545.6757959/1‑91 (MQ=60)
   GGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑C‑Ccga                                                                                            >  SRR4302545.9064985/1‑91 (MQ=60)
    GTAAGAAAACCGTCGAACTGAACTAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGAT                                                                                            >  SRR4302545.9273953/1‑94 (MQ=60)
    GTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGAT                                                                                            <  SRR4302545.6294128/94‑1 (MQ=60)
    GTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑C‑Ccgaa                                                                                           <  SRR4302545.4403694/94‑5 (MQ=60)
     TAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATG                                                                                           >  SRR4302545.3341872/1‑94 (MQ=60)
     GAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATG                                                                                           <  SRR4302545.3296310/94‑1 (MQ=60)
     GAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATG                                                                                           <  SRR4302545.9051267/94‑1 (MQ=60)
      AAGAAATCCGTCGAACTGAACGAAAGCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGC                                                                                          <  SRR4302545.3799406/94‑1 (MQ=60)
      AAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGC                                                                                          >  SRR4302545.6774064/1‑94 (MQ=60)
      AAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGC                                                                                          >  SRR4302545.4299645/1‑94 (MQ=60)
      AAAAAATCCGTCGAACTGAACGAAAACGTCCTGCCGGCGC‑GCGCAGCGTGGATCCAATTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGC                                                                                          <  SRR4302545.1617799/94‑1 (MQ=60)
       AGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCT                                                                                         >  SRR4302545.9397693/1‑94 (MQ=60)
       AGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCT                                                                                         >  SRR4302545.9397694/1‑94 (MQ=60)
       AGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCT                                                                                         >  SRR4302545.8116757/1‑94 (MQ=60)
        GAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGAGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTC                                                                                        <  SRR4302545.5939272/94‑1 (MQ=60)
        AAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTGCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTC                                                                                        <  SRR4302545.3207602/94‑1 (MQ=60)
         AAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCG                                                                                       <  SRR4302545.1133786/94‑1 (MQ=60)
         AAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCG                                                                                       <  SRR4302545.325657/94‑1 (MQ=60)
          AAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGG                                                                                      >  SRR4302545.3815114/1‑94 (MQ=60)
          AAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGG                                                                                      >  SRR4302545.7564661/1‑94 (MQ=60)
           AACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGT                                                                                     >  SRR4302545.4439716/1‑94 (MQ=60)
           AACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGT                                                                                     >  SRR4302545.10217898/1‑94 (MQ=60)
            ACCGTCGAACTGAACTAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCTGTT                                                                                    <  SRR4302545.8415414/94‑1 (MQ=60)
            ACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTT                                                                                    >  SRR4302545.2083/1‑94 (MQ=60)
            ACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTT                                                                                    <  SRR4302545.10934295/94‑1 (MQ=60)
             CCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTC                                                                                   <  SRR4302545.10354903/94‑1 (MQ=60)
              CGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCC                                                                                  >  SRR4302545.9175605/1‑94 (MQ=60)
              CGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCC                                                                                  <  SRR4302545.4143457/94‑1 (MQ=60)
               GTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCA                                                                                 >  SRR4302545.5861305/1‑94 (MQ=60)
               GTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCA                                                                                 >  SRR4302545.9890764/1‑94 (MQ=60)
               GTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCA                                                                                 <  SRR4302545.898541/94‑1 (MQ=60)
                TCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAA                                                                                <  SRR4302545.7913282/94‑1 (MQ=60)
                 CGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAAT                                                                               >  SRR4302545.1257220/1‑94 (MQ=60)
                 CGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAAT                                                                               <  SRR4302545.733849/94‑1 (MQ=60)
                   AACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATAC                                                                             <  SRR4302545.7996002/94‑1 (MQ=60)
                    ACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACC                                                                            >  SRR4302545.4079999/1‑94 (MQ=60)
                    ACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACC                                                                            <  SRR4302545.7584316/94‑1 (MQ=60)
                     CTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCG                                                                           <  SRR4302545.3502687/94‑1 (MQ=60)
                     CTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCG                                                                           <  SRR4302545.3151042/94‑1 (MQ=60)
                     CTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCG                                                                           >  SRR4302545.3670005/1‑94 (MQ=60)
                      TGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGT                                                                          >  SRR4302545.1619148/1‑94 (MQ=60)
                      TGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGT                                                                          <  SRR4302545.1743931/94‑1 (MQ=60)
                      TGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGT                                                                          >  SRR4302545.2396023/1‑94 (MQ=60)
                         ACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCA                                                                       >  SRR4302545.3504438/1‑94 (MQ=60)
                         ACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCA                                                                       >  SRR4302545.3504437/1‑94 (MQ=60)
                         ACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCA                                                                       >  SRR4302545.7643572/1‑94 (MQ=60)
                          CGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAG                                                                      <  SRR4302545.2237040/94‑1 (MQ=60)
                          CGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAG                                                                      <  SRR4302545.5390585/94‑1 (MQ=60)
                          CGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAG                                                                      <  SRR4302545.4760408/94‑1 (MQ=60)
                           GAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGG                                                                     >  SRR4302545.7017202/1‑94 (MQ=60)
                           GAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGG                                                                     >  SRR4302545.9635190/1‑94 (MQ=60)
                           GAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGG                                                                     >  SRR4302545.5216267/1‑94 (MQ=60)
                           GAAAACGGCGTTCCGGCGC‑GCGCAGCGTCGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATTCCGTGCAGG                                                                     <  SRR4302545.4177039/94‑1 (MQ=60)
                              AACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATC                                                                  <  SRR4302545.4616239/94‑1 (MQ=60)
                              AACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATC                                                                  <  SRR4302545.7860791/94‑1 (MQ=60)
                              AACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATC                                                                  <  SRR4302545.320944/94‑1 (MQ=60)
                               ACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCT                                                                 <  SRR4302545.4119504/94‑1 (MQ=60)
                               ACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCT                                                                 <  SRR4302545.9945606/94‑1 (MQ=60)
                                CGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTG                                                                <  SRR4302545.10142942/94‑1 (MQ=60)
                                 GGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGA                                                               >  SRR4302545.4049414/1‑94 (MQ=60)
                                 GGCGTTACGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGA                                                               >  SRR4302545.10835573/1‑94 (MQ=60)
                                  GCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGAT                                                              <  SRR4302545.8549993/94‑1 (MQ=60)
                                  GCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGAT                                                              >  SRR4302545.798673/1‑94 (MQ=60)
                                   CGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATG                                                             <  SRR4302545.1839379/94‑1 (MQ=60)
                                   CGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATG                                                             >  SRR4302545.8901141/1‑94 (MQ=60)
                                   CGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATG                                                             <  SRR4302545.2635923/94‑1 (MQ=60)
                                   CGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATG                                                             <  SRR4302545.10157881/94‑1 (MQ=60)
                                   CGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATG                                                             <  SRR4302545.4533641/94‑1 (MQ=60)
                                   CGTTCCGGCG‑AGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATG                                                             >  SRR4302545.1585274/1‑94 (MQ=60)
                                    cataccggcgcGCGCAGCGTGGATCCAGTTTCTGATCATCATCCCGCTGATGATTATCC‑CGATGCTCGGTACCAATACCGTGCAGGATCTGATG                                                             <  SRR4302545.395257/83‑1 (MQ=60)
                                    GTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTTATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGA                                                            >  SRR4302545.38747/1‑94 (MQ=60)
                                    GTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGA                                                            >  SRR4302545.7908869/1‑94 (MQ=60)
                                    GTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGA                                                            >  SRR4302545.1276261/1‑94 (MQ=60)
                                    GTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGA                                                            >  SRR4302545.862381/1‑94 (MQ=60)
                                    GTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGA                                                            <  SRR4302545.5967104/94‑1 (MQ=60)
                                     TTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAA                                                           <  SRR4302545.1058748/94‑1 (MQ=60)
                                      TCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAAT                                                          >  SRR4302545.10833715/1‑94 (MQ=60)
                                      TCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAAT                                                          <  SRR4302545.2044373/94‑1 (MQ=60)
                                        CGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAC                                                        >  SRR4302545.1467027/1‑94 (MQ=60)
                                        CGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAC                                                        <  SRR4302545.7551772/94‑1 (MQ=60)
                                        CGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAC                                                        >  SRR4302545.3056142/1‑94 (MQ=60)
                                         GGCGC‑GCGCAGCGTGGATCTAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACT                                                       <  SRR4302545.1837478/94‑1 (MQ=60)
                                          GCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTA                                                      <  SRR4302545.4423925/94‑1 (MQ=60)
                                           gcgcgctCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTA                                                      <  SRR4302545.4424229/87‑1 (MQ=60)
                                             gcGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATT                                                    <  SRR4302545.8237738/92‑1 (MQ=60)
                                             gcGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATT                                                    <  SRR4302545.754387/92‑1 (MQ=60)
                                             gcGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATT                                                    <  SRR4302545.6252803/92‑1 (MQ=60)
                                             gcGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATT                                                    <  SRR4302545.4269449/92‑1 (MQ=60)
                                              CGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTA                                                   <  SRR4302545.1108802/94‑1 (MQ=60)
                                              CGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTA                                                   >  SRR4302545.8642330/1‑94 (MQ=60)
                                              CGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTA                                                   >  SRR4302545.1895163/1‑94 (MQ=60)
                                                CGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATT                                                 >  SRR4302545.7235230/1‑94 (MQ=60)
                                                CGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATT                                                 >  SRR4302545.10141994/1‑94 (MQ=60)
                                                CGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATT                                                 <  SRR4302545.1403510/94‑1 (MQ=60)
                                                CGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATT                                                 <  SRR4302545.2459524/94‑1 (MQ=60)
                                                CGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATT                                                 >  SRR4302545.2646171/1‑94 (MQ=60)
                                                CGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATT                                                 <  SRR4302545.297731/94‑1 (MQ=60)
                                                 GCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTA                                                >  SRR4302545.5509334/1‑94 (MQ=60)
                                                  CAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAA                                               <  SRR4302545.9552674/94‑1 (MQ=60)
                                                   AGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAAT                                              >  SRR4302545.6628983/1‑94 (MQ=60)
                                                   AGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAAT                                              >  SRR4302545.1719325/1‑94 (MQ=60)
                                                    GCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATA                                             <  SRR4302545.3022422/94‑1 (MQ=60)
                                                    GCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATA                                             <  SRR4302545.6369824/94‑1 (MQ=60)
                                                     CGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATAT                                            <  SRR4302545.4382555/94‑1 (MQ=60)
                                                     CGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATAT                                            >  SRR4302545.6028147/1‑94 (MQ=60)
                                                     CGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATAT                                            >  SRR4302545.1534543/1‑94 (MQ=60)
                                                     CGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATAT                                            >  SRR4302545.1162672/1‑94 (MQ=60)
                                                      GTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATG                                           >  SRR4302545.8792803/1‑94 (MQ=60)
                                                      GTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATG                                           <  SRR4302545.2997886/94‑1 (MQ=60)
                                                      GTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATG                                           >  SRR4302545.3727442/1‑94 (MQ=60)
                                                      GTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATG                                           >  SRR4302545.8964889/1‑94 (MQ=60)
                                                       TGGATCCTGTTGCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGGTCCAATACCGTGCAGGATCTGATGAAGACTATTATTAATATGA                                          >  SRR4302545.9687457/1‑94 (MQ=60)
                                                       TGGATCCAGTTCGTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGA                                          <  SRR4302545.7132885/94‑1 (MQ=60)
                                                       TGGATCCAGTTCCTGCTCGTCATCCCGCTGCTGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGACTACTTTTATTATTATGC                                          >  SRR4302545.8751253/1‑94 (MQ=60)
                                                       TGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGACCTGATGAATACTATTATTAATATGA                                          <  SRR4302545.10450734/94‑1 (MQ=60)
                                                        GGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTGTTAATATGAC                                         >  SRR4302545.1959825/1‑94 (MQ=60)
                                                        GGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAC                                         >  SRR4302545.1481605/1‑94 (MQ=60)
                                                        GGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAC                                         >  SRR4302545.9600581/1‑94 (MQ=60)
                                                        GGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAC                                         >  SRR4302545.8130368/1‑94 (MQ=60)
                                                         gcaCCAGTTCCTGATCGTCATCCCGCTGATGATTGTCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGTTGAATACTATTATTAATATGACA                                        <  SRR4302545.9621569/91‑1 (MQ=60)
                                                         GATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACC                                        <  SRR4302545.10232459/94‑1 (MQ=60)
                                                         GATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACC                                        >  SRR4302545.8434250/1‑94 (MQ=60)
                                                         GATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACC                                        <  SRR4302545.10158684/94‑1 (MQ=60)
                                                         GATCCAGTTCCTGATCGTCATCCCGCCGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACC                                        <  SRR4302545.2902772/94‑1 (MQ=60)
                                                          ATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCG                                       >  SRR4302545.10463681/1‑94 (MQ=60)
                                                          ATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCG                                       <  SRR4302545.6855260/94‑1 (MQ=60)
                                                          ATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCG                                       <  SRR4302545.1181401/94‑1 (MQ=60)
                                                          ATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCG                                       <  SRR4302545.1154152/94‑1 (MQ=60)
                                                           TCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGC                                      >  SRR4302545.9058450/1‑94 (MQ=60)
                                                           TCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGC                                      <  SRR4302545.6890474/94‑1 (MQ=60)
                                                           TCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGC                                      >  SRR4302545.5621371/1‑94 (MQ=60)
                                                           TCCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGC                                      >  SRR4302545.1278098/1‑94 (MQ=60)
                                                            CCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCC                                     <  SRR4302545.1084943/94‑1 (MQ=60)
                                                            CCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCC                                     <  SRR4302545.8758683/94‑1 (MQ=60)
                                                            CCAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCC                                     <  SRR4302545.5523866/94‑1 (MQ=60)
                                                             CAGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCG                                    <  SRR4302545.7735165/94‑1 (MQ=60)
                                                              AGTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGC                                   >  SRR4302545.3902735/1‑94 (MQ=60)
                                                               GTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCA                                  >  SRR4302545.5730308/1‑94 (MQ=60)
                                                               GTTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCA                                  <  SRR4302545.4600356/94‑1 (MQ=60)
                                                                TTCCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAG                                 <  SRR4302545.2767709/94‑1 (MQ=60)
                                                                  CCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCG                               >  SRR4302545.855586/1‑94 (MQ=60)
                                                                  CCTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCG                               <  SRR4302545.10686602/94‑1 (MQ=60)
                                                                   CTGATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGT                              >  SRR4302545.2600219/1‑94 (MQ=60)
                                                                     GATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCC                            >  SRR4302545.4104614/1‑94 (MQ=60)
                                                                     GATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACAGCCGCAGCGTCA                            >  SRR4302545.9392679/1‑94 (MQ=60)
                                                                     GATCGTCATCCCGCTGATGATTAT‑CGCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCC                            >  SRR4302545.6404851/1‑94 (MQ=60)
                                                                      ATCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCA                           >  SRR4302545.2398122/1‑94 (MQ=60)
                                                                       TCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCAT                          <  SRR4302545.10600639/94‑1 (MQ=60)
                                                                       TCGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCAT                          >  SRR4302545.7010042/1‑94 (MQ=60)
                                                                        CGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATG                         <  SRR4302545.8015747/94‑1 (MQ=60)
                                                                        CGTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATG                         <  SRR4302545.8986787/94‑1 (MQ=60)
                                                                         GTCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGC                        >  SRR4302545.6921922/1‑94 (MQ=60)
                                                                          TCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCT                       <  SRR4302545.3018070/94‑1 (MQ=60)
                                                                          TCATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCT                       >  SRR4302545.10391256/1‑94 (MQ=60)
                                                                           CATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTT                      >  SRR4302545.10262084/1‑94 (MQ=60)
                                                                           CATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTT                      >  SRR4302545.10751955/1‑94 (MQ=60)
                                                                           CATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGCCCATGCTT                      >  SRR4302545.2366154/1‑94 (MQ=60)
                                                                            CTCCCACTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATTCTATTATTAATATGACCGCCGCAGCGTCCATGCTTC                     <  SRR4302545.2190634/94‑1 (MQ=60)
                                                                            ATCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCAGCAGCGTCCATGCTTC                     >  SRR4302545.6989327/1‑94 (MQ=60)
                                                                             TCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCC                    <  SRR4302545.1135695/94‑1 (MQ=60)
                                                                             CCCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCC                    <  SRR4302545.2359325/94‑1 (MQ=60)
                                                                              CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCG                   >  SRR4302545.5852644/1‑94 (MQ=60)
                                                                              CCCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGCCCATGCTTCCG                   <  SRR4302545.8469/94‑1 (MQ=60)
                                                                               CCGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGC                  <  SRR4302545.2592694/94‑1 (MQ=60)
                                                                                CGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCC                 >  SRR4302545.4779675/1‑94 (MQ=60)
                                                                                CGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCC                 <  SRR4302545.4088681/94‑1 (MQ=60)
                                                                                CGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCC                 >  SRR4302545.5538354/1‑94 (MQ=60)
                                                                                CGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCC                 >  SRR4302545.933997/1‑94 (MQ=60)
                                                                                CGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCC                 >  SRR4302545.5538355/1‑94 (MQ=60)
                                                                                CGCTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCC                 <  SRR4302545.4229345/94‑1 (MQ=60)
                                                                                  CTGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGT               <  SRR4302545.10047606/94‑1 (MQ=60)
                                                                                   TGATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTT              <  SRR4302545.4296431/94‑1 (MQ=60)
                                                                                    GATGATTATCC‑CGATGCTCGGTTACAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTA             <  SRR4302545.6063192/94‑1 (MQ=60)
                                                                                     ATGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGCCCATGCTTCCGCCGTTAT            >  SRR4302545.2429698/1‑94 (MQ=60)
                                                                                      TGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATT           >  SRR4302545.5038051/1‑94 (MQ=60)
                                                                                      TGATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTCCCGCCGTTATT           <  SRR4302545.7055666/94‑1 (MQ=60)
                                                                                       GATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTC          >  SRR4302545.4295536/1‑94 (MQ=60)
                                                                                       GATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTC          >  SRR4302545.2827761/1‑94 (MQ=60)
                                                                                       GATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTC          >  SRR4302545.8132151/1‑94 (MQ=60)
                                                                                       GATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTC          >  SRR4302545.3777407/1‑94 (MQ=60)
                                                                                        ATTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCA         >  SRR4302545.5851597/1‑94 (MQ=60)
                                                                                         TTATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCAT        >  SRR4302545.217450/1‑94 (MQ=60)
                                                                                          TATCC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATC       <  SRR4302545.3350043/94‑1 (MQ=60)
                                                                                           atcC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCA      >  SRR4302545.9342781/4‑94 (MQ=60)
                                                                                            tcC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCAT     <  SRR4302545.8025698/92‑1 (MQ=60)
                                                                                            tcC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCAT     <  SRR4302545.1897949/92‑1 (MQ=60)
                                                                                            tcC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCAT     >  SRR4302545.4873556/3‑94 (MQ=60)
                                                                                            tcC‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCAT     >  SRR4302545.2887562/3‑94 (MQ=60)
                                                                                            tcC‑CGATGCCCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCAT     <  SRR4302545.1897948/92‑1 (MQ=60)
                                                                                            C‑C‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATG    >  SRR4302545.10557940/1‑94 (MQ=60)
                                                                                            C‑C‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATG    <  SRR4302545.1950173/94‑1 (MQ=60)
                                                                                              C‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGC   >  SRR4302545.2637985/1‑94 (MQ=60)
                                                                                                CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCT  >  SRR4302545.8175230/1‑94 (MQ=60)
                                                                                                |                                                                                             
TTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑C‑CGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCT  >  NC_000913/3224832‑3225019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑