Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,224,926	+C	coding (1052/1434 nt)	ygjI →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,224,924	1	.	C	99.1%	758.2 / ‑1.2	217	coding (1050/1434 nt)	ygjI	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (97/117); total (99/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 239 total aligned reads displayed.

CTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑T‑CCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCA  >  NC_000913/3224831‑3225012

 tttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑T‑c                                                                                         >  1:6092620/1‑93 (MQ=255)

 tttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑T‑c                                                                                         >  2:4458879/1‑93 (MQ=255)

  ttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑T‑cc                                                                                        >  2:3172021/1‑93 (MQ=255)

  ttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑T‑cc                                                                                        >  2:1243257/1‑93 (MQ=255)

   tGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑T‑ccc                                                                                       >  1:927166/1‑92 (MQ=255)

   tGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑T‑ccc                                                                                       <  1:6010546/93‑2 (MQ=255)

   tGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑T‑ccc                                                                                       <  1:346001/93‑2 (MQ=255)

     gTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCTTGATCGTCATCCCGCTGATGATTA‑T‑cccga                                                                                     <  2:2898121/93‑4 (MQ=255)

     gTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑T‑cccga                                                                                     <  1:539869/93‑4 (MQ=255)

      tAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑T‑cccgat                                                                                    >  1:3606655/1‑89 (MQ=255)

        aGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGc                                                                                   <  1:284296/93‑1 (MQ=255)

        aGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGc                                                                                   >  1:5518978/1‑93 (MQ=255)

        aGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGc                                                                                   >  2:1313048/1‑93 (MQ=255)

         gAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCt                                                                                  >  1:2705109/1‑93 (MQ=255)

           aaaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCg                                                                                >  2:1147171/1‑93 (MQ=255)

            aaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCgg                                                                               >  2:740437/1‑93 (MQ=255)

             aCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGt                                                                              <  1:1515538/93‑1 (MQ=255)

              ccGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGGTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGtt                                                                             <  1:3468368/93‑1 (MQ=255)

               cGTCGAGCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTc                                                                            >  2:555/1‑93 (MQ=255)

                gTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTcc                                                                           >  2:3244536/1‑93 (MQ=255)

                gTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTcc                                                                           <  2:1366514/93‑1 (MQ=255)

                 tCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCa                                                                          >  1:385672/1‑93 (MQ=255)

                   gAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAAt                                                                        >  2:4715077/1‑93 (MQ=255)

                     aCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATAc                                                                      >  2:519618/1‑93 (MQ=255)

                      cTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATAcc                                                                     <  2:2481698/93‑1 (MQ=255)

                      cTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATAcc                                                                     <  1:3162339/93‑1 (MQ=255)

                      cTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATAcc                                                                     >  2:3078493/1‑93 (MQ=255)

                       tGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCg                                                                    <  1:2028764/93‑1 (MQ=255)

                        gAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGt                                                                   <  1:5306055/93‑1 (MQ=255)

                        gAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGt                                                                   <  1:5439576/93‑1 (MQ=255)

                         aaCGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTg                                                                  <  2:5161709/93‑1 (MQ=255)

                            gAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAg                                                               >  1:3914154/1‑93 (MQ=255)

                            gAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAg                                                               >  2:544572/1‑93 (MQ=255)

                            gAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAg                                                               <  2:4870695/93‑1 (MQ=255)

                              aaaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGa                                                             >  2:1959263/1‑93 (MQ=255)

                               aaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGAt                                                            >  2:3962678/1‑93 (MQ=255)

                               aaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGAt                                                            >  1:97539/1‑93 (MQ=255)

                               aaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGAt                                                            >  1:5937538/1‑93 (MQ=255)

                               aaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTACCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGAt                                                            >  2:3136926/1‑93 (MQ=255)

                                aCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGGTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATc                                                           <  1:4558753/93‑1 (MQ=255)

                                aCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATc                                                           >  2:5241464/1‑93 (MQ=255)

                                 cGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCt                                                          <  2:3925559/93‑1 (MQ=255)

                                 cGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCt                                                          <  1:4202828/93‑1 (MQ=255)

                                  ggCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCtg                                                         <  1:819993/93‑1 (MQ=255)

                                  ggCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCtg                                                         >  2:4753068/1‑93 (MQ=255)

                                  ggCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCtg                                                         >  2:807983/1‑93 (MQ=255)

                                   gCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCtga                                                        >  1:6070572/1‑93 (MQ=255)

                                   gCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCtga                                                        <  1:3566733/93‑1 (MQ=255)

                                    cGTGCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgat                                                       <  2:5683365/93‑1 (MQ=255)

                                     gTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatg                                                      >  1:2918217/1‑93 (MQ=255)

                                     gTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatg                                                      >  2:5920580/1‑93 (MQ=255)

                                      ttCCGGCGC‑GCGTAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatga                                                     >  2:4399733/1‑93 (MQ=255)

                                      ttCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatga                                                     >  1:6473792/1‑93 (MQ=255)

                                      ttCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatga                                                     >  1:206435/1‑93 (MQ=255)

                                       tCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGaa                                                    <  1:161302/93‑1 (MQ=255)

                                          ggcgc‑gcgcaGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAc                                                 <  2:5299626/93‑1 (MQ=255)

                                            gcgcgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTACC‑CCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACt                                                >  2:5350899/5‑93 (MQ=255)

                                            gcgcgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACt                                                <  1:2891539/89‑1 (MQ=255)

                                             cgcgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACta                                               >  2:3465951/4‑93 (MQ=255)

                                              gcgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtat                                              <  1:5844068/91‑1 (MQ=255)

                                               cgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtatt                                             <  2:1374353/92‑1 (MQ=255)

                                               cgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtatt                                             <  1:2263960/92‑1 (MQ=255)

                                                gcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtatta                                            <  2:4339177/93‑1 (MQ=255)

                                                 cgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtattat                                           <  1:5266273/93‑1 (MQ=255)

                                                   cAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTAttatta                                         >  2:6050576/1‑93 (MQ=255)

                                                   cAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTAttatta                                         <  1:1687365/93‑1 (MQ=255)

                                                     gCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAat                                       >  1:2586619/1‑93 (MQ=255)

                                                      ggTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAata                                      <  1:2318391/92‑1 (MQ=255)

                                                      cGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAata                                      <  2:1656792/93‑1 (MQ=255)

                                                      cGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAata                                      <  1:5771290/93‑1 (MQ=255)

                                                        tGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATg                                    <  2:194370/93‑1 (MQ=255)

                                                         ggATCCAGTTCCTGATCGTCATCCGGCTGATGATTA‑TCCCGATGCGCGGTTCCAATGCCGTGCAGGATCTGATGAATACTATTATTAATATGa                                   <  2:6353718/93‑1 (MQ=255)

                                                         ggATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGa                                   <  2:6455105/93‑1 (MQ=255)

                                                         ggATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGa                                   <  2:885023/93‑1 (MQ=255)

                                                         ggATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGa                                   <  2:1032026/93‑1 (MQ=255)

                                                         ggATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGa                                   <  1:1526747/93‑1 (MQ=255)

                                                          gATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAc                                  >  1:4288024/1‑93 (MQ=255)

                                                          gATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAc                                  <  1:3942109/93‑1 (MQ=255)

                                                            tCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAccg                                <  2:2865062/93‑1 (MQ=255)

                                                             ccAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAccgc                               >  2:169953/1‑93 (MQ=255)

                                                              cAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAccgcc                              >  2:2326509/1‑93 (MQ=255)

                                                              cAGTTCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAccgcc                              <  1:3224404/93‑1 (MQ=255)

                                                                 ttCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGACGCa                           <  2:5867415/93‑1 (MQ=255)

                                                                  tCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAg                          <  2:1285573/93‑1 (MQ=255)

                                                                  tCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAg                          >  2:2700108/1‑93 (MQ=255)

                                                                  tCCTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAg                          <  1:4144479/93‑1 (MQ=255)

                                                                    cTGATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCg                        >  2:2156922/1‑93 (MQ=255)

                                                                      gATCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTc                      >  2:3814343/1‑93 (MQ=255)

                                                                       aTCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTcc                     >  2:2532456/1‑93 (MQ=255)

                                                                        tCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCa                    >  2:6427851/1‑93 (MQ=255)

                                                                        tCGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCa                    >  1:104889/1‑93 (MQ=255)

                                                                         cGTCATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCAt                   <  1:628500/93‑1 (MQ=255)

                                                                            cATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCt                <  2:4557505/93‑1 (MQ=255)

                                                                            cATCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCt                >  2:4207275/1‑93 (MQ=255)

                                                                             aTCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCtt               >  2:1544199/1‑93 (MQ=255)

                                                                             aTCCCGCTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCtt               >  1:1795529/1‑93 (MQ=255)

                                                                                   cTGATGATTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTccgccg         >  1:2606978/1‑93 (MQ=255)

                                                                                     gatgatTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGtt       <  1:4947777/93‑1 (MQ=255)

                                                                                       tgatTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTAATAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTAt     <  1:3772098/93‑1 (MQ=255)

                                                                                        gatTA‑TCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTAtt    <  2:223724/93‑1 (MQ=255)

CTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTA‑T‑CCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCA  >  NC_000913/3224831‑3225012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCT  >  NC_000913/3224833‑3225019

TTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CC‑C                                                                                              >  SRR4302537.933556/1‑94 (MQ=60)

 TGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCcg                                                                                              <  SRR4302537.348406/94‑3 (MQ=60)

 TGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCcg                                                                                              <  SRR4302537.6051840/94‑3 (MQ=60)

  GGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCcga                                                                                             >  SRR4302537.5449853/1‑91 (MQ=60)

   GTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GAT                                                                                            <  SRR4302537.543585/94‑1 (MQ=60)

   GTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GAT                                                                                            >  SRR4302537.3632076/1‑94 (MQ=60)

   GTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GAT                                                                                            <  SRR4302537.3750466/94‑1 (MQ=60)

    TAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATG                                                                                           >  SRR4302537.6405252/1‑94 (MQ=60)

    TAAGAAAACCATCGAACTGAACGAAAACGGCTTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATG                                                                                           >  SRR4302537.6514243/1‑94 (MQ=60)

     AAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGC                                                                                          >  SRR4302537.5556661/1‑94 (MQ=60)

      AGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCT                                                                                         >  SRR4302537.2723991/1‑94 (MQ=60)

      AGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCT                                                                                         <  SRR4302537.286278/94‑1 (MQ=60)

      AGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCT                                                                                         <  SRR4302537.1536178/94‑1 (MQ=60)

        AAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCG                                                                                       <  SRR4302537.5912544/94‑1 (MQ=60)

        AAAACCGTCGAACTGAACGAAAACGGCGTCCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCG                                                                                       <  SRR4302537.4252811/94‑1 (MQ=60)

         AAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGG                                                                                      >  SRR4302537.4519514/1‑94 (MQ=60)

           ACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTT                                                                                    <  SRR4302537.1526129/94‑1 (MQ=60)

            CCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGGTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTC                                                                                   <  SRR4302537.3492904/94‑1 (MQ=60)

             CGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCC                                                                                  >  SRR4302537.968810/1‑94 (MQ=60)

              GTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCA                                                                                 >  SRR4302537.388339/1‑94 (MQ=60)

                CGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAAT                                                                               <  SRR4302537.3622007/94‑1 (MQ=60)

                CGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAAT                                                                               <  SRR4302537.5323702/94‑1 (MQ=60)

                   ACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACC                                                                            <  SRR4302537.1930457/94‑1 (MQ=60)

                   ACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACC                                                                            <  SRR4302537.3898992/94‑1 (MQ=60)

                    CTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCG                                                                           <  SRR4302537.3184540/94‑1 (MQ=60)

                     TGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGT                                                                          >  SRR4302537.6124619/1‑94 (MQ=60)

                     TGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGT                                                                          <  SRR4302537.2042908/94‑1 (MQ=60)

                      GAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTG                                                                         <  SRR4302537.3412688/94‑1 (MQ=60)

                      GAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTG                                                                         <  SRR4302537.5342520/94‑1 (MQ=60)

                      GAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTG                                                                         <  SRR4302537.5476683/94‑1 (MQ=60)

                         CGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAG                                                                      >  SRR4302537.3941516/1‑94 (MQ=60)

                         CGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAG                                                                      <  SRR4302537.3255069/94‑1 (MQ=60)

                            AAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGAT                                                                   >  SRR4302537.98248/1‑94 (MQ=60)

                            AAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGAT                                                                   >  SRR4302537.5978284/1‑94 (MQ=60)

                            AAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGAT                                                                   >  SRR4302537.1965429/1‑94 (MQ=60)

                            AAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGGTCCTGATCGTCATCCCGCTGATGATTATCCC‑GCTGCTCGGTTCCAATACCGCGCAGGAC                                                                   >  SRR4302537.926243/1‑94 (MQ=60)

                             AACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATC                                                                  <  SRR4302537.5847599/94‑1 (MQ=60)

                              ACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGGTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCT                                                                 <  SRR4302537.4590308/94‑1 (MQ=60)

                               CGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTG                                                                <  SRR4302537.4232169/94‑1 (MQ=60)

                                GGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGA                                                               >  SRR4302537.6112281/1‑94 (MQ=60)

                                GGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGA                                                               <  SRR4302537.825623/94‑1 (MQ=60)

                                 GCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGAT                                                              <  SRR4302537.3591884/94‑1 (MQ=60)

                                  CGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATG                                                             >  SRR4302537.2938720/1‑94 (MQ=60)

                                  CGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATG                                                             <  SRR4302537.6434213/94‑1 (MQ=60)

                                  CGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATG                                                             >  SRR4302537.1765606/1‑94 (MQ=60)

                                   GTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGA                                                            >  SRR4302537.207914/1‑94 (MQ=60)

                                   GTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGA                                                            >  SRR4302537.6517722/1‑94 (MQ=60)

                                   GTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGA                                                            >  SRR4302537.5381297/1‑94 (MQ=60)

                                     TCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAAT                                                          >  SRR4302537.6044184/1‑94 (MQ=60)

                                     TCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAAT                                                          <  SRR4302537.999375/94‑1 (MQ=60)

                                     TCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAAT                                                          <  SRR4302537.162451/94‑1 (MQ=60)

                                      CCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATA                                                         <  SRR4302537.5637444/94‑1 (MQ=60)

                                         GCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTA                                                      <  SRR4302537.2911839/94‑1 (MQ=60)

                                         GCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTA                                                      >  SRR4302537.433979/1‑94 (MQ=60)

                                          CGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTAT                                                     <  SRR4302537.6024154/94‑1 (MQ=60)

                                            gcGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATT                                                    <  SRR4302537.5884138/92‑1 (MQ=60)

                                            gcGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATT                                                    <  SRR4302537.2056706/92‑1 (MQ=60)

                                             CGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTA                                                   <  SRR4302537.2279939/94‑1 (MQ=60)

                                              GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTAT                                                  <  SRR4302537.6253500/94‑1 (MQ=60)

                                              GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTAT                                                  <  SRR4302537.4214705/94‑1 (MQ=60)

                                               CGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATT                                                 <  SRR4302537.3630674/94‑1 (MQ=60)

                                               CGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATT                                                 >  SRR4302537.1195840/1‑94 (MQ=60)

                                               CGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATT                                                 <  SRR4302537.2767383/94‑1 (MQ=60)

                                               CGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATT                                                 <  SRR4302537.5302511/94‑1 (MQ=60)

                                               CGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATT                                                 <  SRR4302537.3946191/94‑1 (MQ=60)

                                                 CAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAA                                               <  SRR4302537.1699179/94‑1 (MQ=60)

                                                  AGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAAT                                              >  SRR4302537.2604717/1‑94 (MQ=60)

                                                    GGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATAT                                            <  SRR4302537.2334763/94‑1 (MQ=60)

                                                    CGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCTGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATAT                                            <  SRR4302537.3491219/94‑1 (MQ=60)

                                                    CGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATAT                                            <  SRR4302537.5810848/94‑1 (MQ=60)

                                                      TGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGA                                          <  SRR4302537.3109322/94‑1 (MQ=60)

                                                       GGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAC                                         >  SRR4302537.4317832/1‑94 (MQ=60)

                                                       GGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAC                                         >  SRR4302537.2299765/1‑94 (MQ=60)

                                                       GGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAC                                         <  SRR4302537.1537412/94‑1 (MQ=60)

                                                        GATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACC                                        <  SRR4302537.3969678/94‑1 (MQ=60)

                                                         ATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCG                                       >  SRR4302537.4124561/1‑94 (MQ=60)

                                                           CCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCC                                     >  SRR4302537.2921262/1‑94 (MQ=60)

                                                           CCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCC                                     >  SRR4302537.2090241/1‑94 (MQ=60)

                                                           CCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCC                                     >  SRR4302537.2098828/1‑94 (MQ=60)

                                                            CAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCG                                    <  SRR4302537.3247096/94‑1 (MQ=60)

                                                            CAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCG                                    <  SRR4302537.1559976/94‑1 (MQ=60)

                                                               TTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCCGGATCTGATGAATACTATTATTAATATGACCGCCGCAG                                 >  SRR4302537.4942893/1‑94 (MQ=60)

                                                                TCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGC                                <  SRR4302537.6089023/94‑1 (MQ=60)

                                                                TCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGC                                <  SRR4302537.3870881/94‑1 (MQ=60)

                                                                TCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGC                                <  SRR4302537.4173391/94‑1 (MQ=60)

                                                                   TGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTC                             >  SRR4302537.77833/1‑94 (MQ=60)

                                                                    GATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCC                            <  SRR4302537.781382/94‑1 (MQ=60)

                                                                    GATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCC                            >  SRR4302537.2623370/1‑94 (MQ=60)

                                                                    GATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCC                            >  SRR4302537.5453983/1‑94 (MQ=60)

                                                                     ATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCA                           >  SRR4302537.105643/1‑94 (MQ=60)

                                                                     ATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCA                           >  SRR4302537.3934365/1‑94 (MQ=60)

                                                                       CGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATG                         <  SRR4302537.632780/94‑1 (MQ=60)

                                                                       CGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATG                         <  SRR4302537.5511353/94‑1 (MQ=60)

                                                                       CGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATG                         <  SRR4302537.523193/94‑1 (MQ=60)

                                                                       CGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATG                         >  SRR4302537.2745303/1‑94 (MQ=60)

                                                                         TCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCT                       <  SRR4302537.5037722/94‑1 (MQ=60)

                                                                         TCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCT                       <  SRR4302537.4064701/94‑1 (MQ=60)

                                                                          CATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTT                      >  SRR4302537.268831/1‑94 (MQ=60)

                                                                          CATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTT                      >  SRR4302537.1575748/1‑94 (MQ=60)

                                                                          CATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTT                      >  SRR4302537.1808060/1‑94 (MQ=60)

                                                                            TCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCC                    <  SRR4302537.2362332/94‑1 (MQ=60)

                                                                            TCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCC                    <  SRR4302537.2613900/94‑1 (MQ=60)

                                                                                GCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCG                >  SRR4302537.2625202/1‑94 (MQ=60)

                                                                                 CTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGT               >  SRR4302537.322353/1‑94 (MQ=60)

                                                                                 CTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGT               >  SRR4302537.6189378/1‑94 (MQ=60)

                                                                                  TGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTT              >  SRR4302537.3061749/1‑94 (MQ=60)

                                                                                   GATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTA             <  SRR4302537.4981888/94‑1 (MQ=60)

                                                                                    ATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTAT            >  SRR4302537.3507451/1‑94 (MQ=60)

                                                                                     TGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTAATAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATT           <  SRR4302537.3798579/94‑1 (MQ=60)

                                                                                       ATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCA         >  SRR4302537.6394478/1‑94 (MQ=60)

                                                                                        TTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCAT        <  SRR4302537.318287/94‑1 (MQ=60)

                                                                                        TTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCAT        >  SRR4302537.5563812/1‑94 (MQ=60)

                                                                                        TTAT‑CCTGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCAT        <  SRR4302537.2892070/94‑1 (MQ=60)

                                                                                         TATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATC       >  SRR4302537.1820062/1‑94 (MQ=60)

                                                                                           tcCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCAT     <  SRR4302537.2393723/92‑1 (MQ=60)

                                                                                           C‑CC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATG    >  SRR4302537.3013110/1‑94 (MQ=60)

                                                                                             CC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGC   >  SRR4302537.3952455/1‑94 (MQ=60)

                                                                                              C‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCT  >  SRR4302537.5922189/1‑94 (MQ=60)

                                                                                              C‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCT  <  SRR4302537.3201771/94‑1 (MQ=60)

TTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCT  >  NC_000913/3224833‑3225019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`