Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,980,918 2 bp→CG coding (348‑349/1236 nt) aslB → putative arylsulfatase regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,980,9180GC100.0% 2274.7 / NA 647W116C (TGG→TGC) aslBputative arylsulfatase regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (286/361);  total (286/361)
*NC_0009133,980,9190CG99.9% 2260.5 / NA 654P117A (CCG→GCG) aslBputative arylsulfatase regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (286/367);  minor base T (0/1);  total (286/368)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 675 total aligned reads displayed.

CGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGGCCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTG  >  NC_000913/3980826‑3981006
                                                                                            ||                                                                                       
    ccgtAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGtt                                                                                     >  2:10521402/1‑94 (MQ=255)
      gtAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtc                                                                                   <  1:7338303/94‑1 (MQ=255)
      gtAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtc                                                                                   <  2:1842016/94‑1 (MQ=255)
          cgcTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcgg                                                                               >  1:7952918/1‑93 (MQ=255)
          cgcTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcgc                                                                               <  1:3976907/94‑1 (MQ=255)
          cgcTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcgc                                                                               <  1:5268996/94‑1 (MQ=255)
          cgcTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcgc                                                                               <  1:21935/94‑1 (MQ=255)
           gcTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcgcg                                                                              >  1:9053405/1‑94 (MQ=255)
           gcTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcgcg                                                                              <  2:8242803/94‑1 (MQ=255)
           gcTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcgcg                                                                              <  1:1687795/94‑1 (MQ=255)
            cTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCgg                                                                             <  2:7373239/94‑1 (MQ=255)
             tACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGa                                                                            <  2:8373890/94‑1 (MQ=255)
             tACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGa                                                                            <  2:6373808/94‑1 (MQ=255)
              aCAGGCGAAATATGGTGCTGGCAGGTAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAg                                                                           <  2:4720931/94‑1 (MQ=255)
              aCAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAg                                                                           <  1:4615518/94‑1 (MQ=255)
                aGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGca                                                                         <  2:6056515/94‑1 (MQ=255)
                aGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGca                                                                         <  2:1033209/94‑1 (MQ=255)
                aGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGca                                                                         <  2:9609846/94‑1 (MQ=255)
                aGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGca                                                                         <  1:889500/94‑1 (MQ=255)
                 ggCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcat                                                                        <  1:7244449/94‑1 (MQ=255)
                 ggCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcat                                                                        >  2:7302629/1‑94 (MQ=255)
                  gCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcatc                                                                       <  1:6443793/94‑1 (MQ=255)
                   cGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcatca                                                                      >  2:6969242/1‑94 (MQ=255)
                   cGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcatca                                                                      >  2:3355663/1‑94 (MQ=255)
                    gAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcatcat                                                                     <  1:10011711/94‑1 (MQ=255)
                     gaaTATGGTGCTTGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCAtt                                                                    <  2:3521290/93‑1 (MQ=255)
                     aaaTATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCAtt                                                                    <  2:9047253/94‑1 (MQ=255)
                     aaaTATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCAtt                                                                    >  1:5961416/1‑94 (MQ=255)
                       atatGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCAtttt                                                                  >  1:3087884/1‑94 (MQ=255)
                        tatGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTc                                                                 <  1:5065136/94‑1 (MQ=255)
                        aatGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTc                                                                 <  1:4353469/93‑1 (MQ=255)
                         atGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCt                                                                >  1:3318773/1‑94 (MQ=255)
                          tGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCtt                                                               <  2:860103/94‑1 (MQ=255)
                           ggTGCTGTCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCttg                                                              <  1:9707291/94‑1 (MQ=255)
                           ggTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCttg                                                              >  2:6660934/1‑94 (MQ=255)
                           ggTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCttg                                                              <  1:1212014/94‑1 (MQ=255)
                            gTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCttgt                                                             <  1:10316364/94‑1 (MQ=255)
                             tGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCttgtt                                                            >  1:383636/1‑94 (MQ=255)
                                tGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGt                                                         >  2:8688815/1‑94 (MQ=255)
                                tGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGt                                                         <  1:9137503/94‑1 (MQ=255)
                                 ggCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGtt                                                        >  1:2075696/1‑94 (MQ=255)
                                  gCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGttt                                                       <  1:8081789/94‑1 (MQ=255)
                                  gCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGttt                                                       <  2:159894/94‑1 (MQ=255)
                                   cAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTa                                                      >  1:10596081/1‑94 (MQ=255)
                                    aGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAt                                                     >  2:4934731/1‑94 (MQ=255)
                                    aGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAt                                                     >  1:349230/1‑94 (MQ=255)
                                     ggAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATc                                                    <  1:5763330/94‑1 (MQ=255)
                                     ggAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATc                                                    >  1:2393745/1‑94 (MQ=255)
                                      gAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCg                                                   <  1:6083586/94‑1 (MQ=255)
                                      gAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCg                                                   <  2:1397282/94‑1 (MQ=255)
                                      gAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCg                                                   <  2:3111205/94‑1 (MQ=255)
                                       aaGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGc                                                  >  1:1386129/1‑94 (MQ=255)
                                         gATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTg                                                >  1:9322709/1‑94 (MQ=255)
                                           tAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGa                                              <  2:9318941/94‑1 (MQ=255)
                                           tAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGa                                              >  1:6184045/1‑94 (MQ=255)
                                           tAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGa                                              <  2:4300061/94‑1 (MQ=255)
                                           tAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGa                                              <  1:6639755/94‑1 (MQ=255)
                                            aaGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGAt                                             <  2:5731267/94‑1 (MQ=255)
                                            aaGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGAt                                             >  1:86612/1‑94 (MQ=255)
                                            aaGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGAt                                             <  2:789505/94‑1 (MQ=255)
                                            aaGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGAt                                             <  2:516523/94‑1 (MQ=255)
                                             aGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATg                                            >  2:4135796/1‑94 (MQ=255)
                                              gTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATgg                                           >  1:2816463/1‑94 (MQ=255)
                                               tAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGc                                          >  2:6471806/1‑94 (MQ=255)
                                                 aCAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGccc                                        >  1:9513273/1‑94 (MQ=255)
                                                 aCAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGccc                                        <  1:2669985/94‑1 (MQ=255)
                                                  cAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCg                                       <  2:2259483/94‑1 (MQ=255)
                                                  cAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCg                                       <  2:2855722/94‑1 (MQ=255)
                                                   aGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGc                                      >  1:5637793/1‑94 (MQ=255)
                                                     cTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCt                                    <  2:2151518/94‑1 (MQ=255)
                                                      ttCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTg                                   <  1:825777/94‑1 (MQ=255)
                                                         cAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTgaga                                >  2:1116740/1‑94 (MQ=255)
                                                              tAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCcac                           >  2:2699885/1‑94 (MQ=255)
                                                              tAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCcac                           <  2:7724349/94‑1 (MQ=255)
                                                              tAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCcac                           >  1:9638227/1‑94 (MQ=255)
                                                                aCGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaa                         <  1:6848332/94‑1 (MQ=255)
                                                                 cGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaat                        >  2:384982/1‑94 (MQ=255)
                                                                 cGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaat                        >  2:1969044/1‑94 (MQ=255)
                                                                   gCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaatca                      <  1:4889199/94‑1 (MQ=255)
                                                                    cGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaatcaa                     >  2:10033113/1‑94 (MQ=255)
                                                                     gTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaatcaat                    <  1:5532039/94‑1 (MQ=255)
                                                                     gTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaatcaat                    >  2:3699064/1‑94 (MQ=255)
                                                                     gTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaatcaat                    >  1:7532000/1‑94 (MQ=255)
                                                                       gctgctGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAatat                  <  2:2557761/94‑1 (MQ=255)
                                                                         tgctgGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATcg                <  1:7722397/94‑1 (MQ=255)
                                                                          gctgGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATcgc               >  1:4543514/1‑94 (MQ=255)
                                                                            tgGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGt             >  1:6301513/1‑94 (MQ=255)
                                                                            tgGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGt             >  2:4038402/1‑94 (MQ=255)
                                                                             ggATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTg            <  2:7514006/94‑1 (MQ=255)
                                                                             ggATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTg            <  1:3739604/94‑1 (MQ=255)
                                                                             ggATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCATGAGATCCACAATCAATATCGCGTg            <  1:4077452/94‑1 (MQ=255)
                                                                              gATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGa           >  1:8468071/1‑94 (MQ=255)
                                                                                tGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACt         <  1:4275402/94‑1 (MQ=255)
                                                                                   cGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTaaa      >  1:193062/1‑94 (MQ=255)
                                                                                     aaTGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAgg    <  2:6581697/94‑1 (MQ=255)
                                                                                      aTGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGt   <  2:4594823/94‑1 (MQ=255)
                                                                                      aTGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGt   <  1:1532614/94‑1 (MQ=255)
                                                                                      aTGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGt   <  2:9878764/94‑1 (MQ=255)
                                                                                       tggtgcGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTg  <  1:4763309/94‑1 (MQ=255)
                                                                                       tggtgcGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTg  <  2:5386209/94‑1 (MQ=255)
                                                                                            ||                                                                                       
CGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGGCCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTG  >  NC_000913/3980826‑3981006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 330 total aligned reads displayed.
TCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCCGGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTA  >  NC_000913/3987453‑3987639
                                                                                             |                                                                                             
TCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCG                                                                                               >  SRR4302545.8304416/1‑94 (MQ=60)
CCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCG                                                                                               <  SRR4302545.1394158/94‑1 (MQ=60)
 CCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGC                                                                                              <  SRR4302545.10092546/94‑1 (MQ=60)
  CGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCC                                                                                             >  SRR4302545.7826164/1‑94 (MQ=60)
   GTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCG                                                                                            <  SRR4302545.6752848/94‑1 (MQ=60)
    TTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGC                                                                                           >  SRR4302545.2014697/1‑94 (MQ=60)
    TTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTGATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGC                                                                                           <  SRR4302545.7256432/94‑1 (MQ=60)
     TATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCTATTTGTTCGGCATCTTGCGGCGCGCCGCG                                                                                          <  SRR4302545.4102573/94‑1 (MQ=60)
     TATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCG                                                                                          >  SRR4302545.760458/1‑94 (MQ=60)
     TATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCG                                                                                          >  SRR4302545.3212722/1‑94 (MQ=60)
     TATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCG                                                                                          >  SRR4302545.8503857/1‑94 (MQ=60)
      ATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGG                                                                                         >  SRR4302545.6629567/1‑94 (MQ=60)
      ATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGG                                                                                         >  SRR4302545.7907404/1‑94 (MQ=60)
        AGGCTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCG                                                                                       <  SRR4302545.3007661/94‑1 (MQ=60)
        AGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGGG                                                                                       >  SRR4302545.6501053/1‑94 (MQ=60)
        AGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCG                                                                                       >  SRR4302545.9073109/1‑94 (MQ=60)
        AGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCG                                                                                       <  SRR4302545.7042149/94‑1 (MQ=60)
        AGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCG                                                                                       >  SRR4302545.8780904/1‑94 (MQ=60)
        AGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCG                                                                                       >  SRR4302545.2817509/1‑94 (MQ=60)
        AGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCG                                                                                       <  SRR4302545.955322/94‑1 (MQ=60)
         GACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGT                                                                                      >  SRR4302545.3777087/1‑94 (MQ=60)
          acgttagagagaATCTCGCGCGCGCCGTTATTCAGAAGCTCTTCTGCCAGCGCAATCCTCATTTGGTCGGCATCTTGCGGCGCGCCGCGGCGTT                                                                                     >  SRR4302545.8833062/13‑94 (MQ=60)
          ACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTTCGGCGCGCCGCGGCGTT                                                                                     <  SRR4302545.1922904/94‑1 (MQ=60)
          ACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTT                                                                                     >  SRR4302545.7944987/1‑94 (MQ=60)
          ACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTT                                                                                     >  SRR4302545.8031406/1‑94 (MQ=60)
          ACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTT                                                                                     >  SRR4302545.2461582/1‑94 (MQ=60)
          ACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTT                                                                                     <  SRR4302545.8548587/94‑1 (MQ=60)
            TTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCA                                                                                   >  SRR4302545.6959167/1‑94 (MQ=60)
             TCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCAC                                                                                  >  SRR4302545.5452910/1‑94 (MQ=60)
             TCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCAC                                                                                  <  SRR4302545.4006994/94‑1 (MQ=60)
              CAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACC                                                                                 >  SRR4302545.6685802/1‑94 (MQ=60)
               AGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCG                                                                                <  SRR4302545.7271145/94‑1 (MQ=60)
               AGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCG                                                                                <  SRR4302545.1713454/94‑1 (MQ=60)
                 CGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCTGCGGCGTTCTTCGCG                                                                              >  SRR4302545.9679539/1‑94 (MQ=60)
                 CGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCG                                                                              <  SRR4302545.9446363/94‑1 (MQ=60)
                 CGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCG                                                                              <  SRR4302545.5898850/94‑1 (MQ=60)
                 CGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCG                                                                              <  SRR4302545.2932304/94‑1 (MQ=60)
                 CGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCG                                                                              <  SRR4302545.2336851/94‑1 (MQ=60)
                  GAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGA                                                                             >  SRR4302545.8502105/1‑94 (MQ=60)
                   AGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAA                                                                            <  SRR4302545.4243560/94‑1 (MQ=60)
                      GTCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAA                                                                         <  SRR4302545.9099653/94‑1 (MQ=60)
                       gccgcgGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAAT                                                                        >  SRR4302545.2042396/7‑94 (MQ=60)
                       TCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAAT                                                                        <  SRR4302545.6677085/94‑1 (MQ=60)
                       TCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAAT                                                                        <  SRR4302545.6677086/94‑1 (MQ=60)
                         TCGCTCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGGTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCT                                                                      <  SRR4302545.4035959/94‑1 (MQ=60)
                         TCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCT                                                                      <  SRR4302545.1649331/94‑1 (MQ=60)
                         TCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCT                                                                      <  SRR4302545.4704033/94‑1 (MQ=60)
                         TCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCT                                                                      >  SRR4302545.7160694/1‑94 (MQ=60)
                         TCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCT                                                                      >  SRR4302545.5485740/1‑94 (MQ=60)
                         TCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCT                                                                      <  SRR4302545.3860030/94‑1 (MQ=60)
                         TCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCT                                                                      <  SRR4302545.10295846/94‑1 (MQ=60)
                         TCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCA                                                                      <  SRR4302545.6818186/94‑1 (MQ=60)
                          CGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTG                                                                     >  SRR4302545.3308024/1‑94 (MQ=60)
                          CGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTG                                                                     <  SRR4302545.1480312/94‑1 (MQ=60)
                          CGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTG                                                                     <  SRR4302545.6518685/94‑1 (MQ=60)
                          CGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCAAATAATCTG                                                                     >  SRR4302545.10835109/1‑94 (MQ=60)
                           GCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTtt                                                                    >  SRR4302545.568818/1‑92 (MQ=60)
                           GCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGC                                                                    >  SRR4302545.8276730/1‑94 (MQ=60)
                           GCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGC                                                                    >  SRR4302545.1897691/1‑94 (MQ=60)
                            CGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCG                                                                   >  SRR4302545.7374850/1‑94 (MQ=60)
                              CGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAA                                                                 >  SRR4302545.7876803/1‑94 (MQ=60)
                               GCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAAC                                                                <  SRR4302545.1773598/94‑1 (MQ=60)
                                CGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACC                                                               >  SRR4302545.8676594/1‑94 (MQ=60)
                                CGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACC                                                               >  SRR4302545.2118849/1‑94 (MQ=60)
                                CGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACC                                                               >  SRR4302545.4134887/1‑94 (MQ=60)
                                CGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACC                                                               <  SRR4302545.10531206/94‑1 (MQ=60)
                                 GCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCG                                                              <  SRR4302545.10634204/94‑1 (MQ=60)
                                  CCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGT                                                             >  SRR4302545.2236304/1‑94 (MQ=60)
                                  CCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGT                                                             >  SRR4302545.4435137/1‑94 (MQ=60)
                                  CAGTTATTAAGCAGCTCTTCTGCCAGCGAAATACCCATTTGTTCGGCATCTTGCGGCGCGCCGAGGCGCTCACAGCGCATAATCTGCGAccact                                                             >  SRR4302545.1140069/1‑89 (MQ=51)
                                   CGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTC                                                            >  SRR4302545.10468714/1‑94 (MQ=60)
                                   CGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTC                                                            >  SRR4302545.8856260/1‑94 (MQ=60)
                                   CGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTC                                                            <  SRR4302545.2478774/94‑1 (MQ=60)
                                    GTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCC                                                           <  SRR4302545.10039373/94‑1 (MQ=60)
                                    GTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCC                                                           <  SRR4302545.5147541/94‑1 (MQ=60)
                                    GTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCC                                                           >  SRR4302545.766790/1‑94 (MQ=60)
                                    GTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCC                                                           >  SRR4302545.716536/1‑94 (MQ=60)
                                    GTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCC                                                           <  SRR4302545.347523/94‑1 (MQ=60)
                                     TTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCG                                                          >  SRR4302545.7674401/1‑94 (MQ=60)
                                     TTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCG                                                          <  SRR4302545.5048315/94‑1 (MQ=60)
                                     TTATTAAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCTACAGTCACCGCGAATAATCTGCGAACCGTCCG                                                          >  SRR4302545.7085622/1‑94 (MQ=60)
                                      TATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGG                                                         >  SRR4302545.10026381/1‑94 (MQ=60)
                                       ATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGC                                                        >  SRR4302545.9534615/1‑94 (MQ=60)
                                       ATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGC                                                        <  SRR4302545.399465/94‑1 (MQ=60)
                                       ATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGC                                                        >  SRR4302545.9534613/1‑94 (MQ=60)
                                       ATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGC                                                        <  SRR4302545.923310/94‑1 (MQ=60)
                                       ATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGC                                                        <  SRR4302545.8422169/94‑1 (MQ=60)
                                       ATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGC                                                        <  SRR4302545.7073694/94‑1 (MQ=60)
                                        TTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCG                                                       >  SRR4302545.9260795/1‑94 (MQ=60)
                                        TTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTACGGCGTCTTGCGACGCGCCGCGGCGTTCcccgcacataaccttcgagccctccggcg                                                       <  SRR4302545.561173/94‑30 (MQ=60)
                                         TCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCgc                                                      <  SRR4302545.9936154/94‑3 (MQ=60)
                                         TCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCgc                                                      <  SRR4302545.8510692/94‑3 (MQ=60)
                                         TCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCgc                                                      <  SRR4302545.284334/94‑3 (MQ=60)
                                          CAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCgcg                                                     <  SRR4302545.9018232/94‑4 (MQ=60)
                                          CAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCgcg                                                     >  SRR4302545.5256231/1‑91 (MQ=60)
                                          CAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCgag                                                     >  SRR4302545.8127783/1‑91 (MQ=60)
                                          CAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCCCGAATAACCCGCGAACCGTCCGGCtcg                                                     >  SRR4302545.1369720/1‑91 (MQ=60)
                                           AGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCgcgc                                                    >  SRR4302545.1651861/1‑90 (MQ=60)
                                           AGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCgcgc                                                    >  SRR4302545.6338141/1‑90 (MQ=60)
                                            GTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCgcgcc                                                   >  SRR4302545.10301124/1‑89 (MQ=60)
                                            GTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCgcgcc                                                   >  SRR4302545.8851711/1‑89 (MQ=60)
                                             ccgcacttcggccatCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCCCCGCGGCGTTCACCGCGAATAATCTGCGACCCGTCCGGCGCGCCG                                                  <  SRR4302545.3742847/79‑1 (MQ=60)
                                             TAGCTCTTCTGCCGGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCG                                                  <  SRR4302545.10449143/94‑1 (MQ=60)
                                             TAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCG                                                  <  SRR4302545.3743187/94‑1 (MQ=60)
                                              AGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGA                                                 <  SRR4302545.5305762/94‑1 (MQ=60)
                                              AGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATATTGCGGCGCGCCGCGGCGTTAACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGA                                                 >  SRR4302545.6457855/1‑94 (MQ=60)
                                               GCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGAC                                                >  SRR4302545.2964223/1‑94 (MQ=60)
                                               GCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGAC                                                <  SRR4302545.3564522/94‑1 (MQ=60)
                                                CTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACC                                               >  SRR4302545.3394587/1‑94 (MQ=60)
                                                CTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACC                                               >  SRR4302545.10805481/1‑94 (MQ=60)
                                                 TCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCA                                              >  SRR4302545.9214969/1‑94 (MQ=60)
                                                 TCTTCTGCCAGCGAAATCCCCATTTGTGCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCA                                              <  SRR4302545.10224730/94‑1 (MQ=60)
                                                  CTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAG                                             <  SRR4302545.6794029/94‑1 (MQ=60)
                                                  CTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAG                                             >  SRR4302545.6568731/1‑94 (MQ=60)
                                                   TTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGC                                            <  SRR4302545.3703359/94‑1 (MQ=60)
                                                    TCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCG                                           <  SRR4302545.3004635/94‑1 (MQ=60)
                                                    TCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCG                                           <  SRR4302545.5909411/94‑1 (MQ=60)
                                                    TCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCG                                           <  SRR4302545.3809616/94‑1 (MQ=60)
                                                     CTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAAACGTCCGGCGCGCCGACCAGCGC                                          >  SRR4302545.4509918/1‑94 (MQ=60)
                                                      TGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCgccccgaccagccac                                         >  SRR4302545.8756922/1‑79 (MQ=60)
                                                      TGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCA                                         <  SRR4302545.1462224/94‑1 (MQ=60)
                                                       GCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCAC                                        <  SRR4302545.88611/94‑1 (MQ=60)
                                                         CAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCTGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGC                                      <  SRR4302545.10696033/94‑1 (MQ=60)
                                                         CAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGC                                      >  SRR4302545.8029070/1‑94 (MQ=60)
                                                         CAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGC                                      <  SRR4302545.10325934/94‑1 (MQ=60)
                                                         CAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGC                                      >  SRR4302545.5040584/1‑94 (MQ=60)
                                                         CAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGC                                      >  SRR4302545.3382880/1‑94 (MQ=60)
                                                          AGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCA                                     >  SRR4302545.7770192/1‑94 (MQ=60)
                                                          AGCGAAAACCCCATTTGTTCGGCATCTTGCGCCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCA                                     <  SRR4302545.6472461/94‑1 (MQ=60)
                                                           GCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAG                                    <  SRR4302545.5113074/94‑1 (MQ=60)
                                                           GCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAG                                    <  SRR4302545.7985898/94‑1 (MQ=60)
                                                             GAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCC                                  >  SRR4302545.9060998/1‑94 (MQ=60)
                                                             GAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCC                                  >  SRR4302545.1094445/1‑94 (MQ=60)
                                                             GAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCC                                  <  SRR4302545.4779612/94‑1 (MQ=60)
                                                             GAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCC                                  >  SRR4302545.280717/1‑94 (MQ=60)
                                                              AAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCA                                 >  SRR4302545.5431127/1‑94 (MQ=60)
                                                              AAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCA                                 >  SRR4302545.4744540/1‑94 (MQ=60)
                                                              AAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCA                                 >  SRR4302545.10429971/1‑94 (MQ=60)
                                                               AATCCCCATTTGTTCGGCATCTTGCTGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAG                                >  SRR4302545.3509952/1‑94 (MQ=60)
                                                               AATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAG                                <  SRR4302545.8338117/94‑1 (MQ=60)
                                                               AATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAG                                <  SRR4302545.554881/94‑1 (MQ=60)
                                                               AATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAG                                >  SRR4302545.4021681/1‑94 (MQ=60)
                                                                   CCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTT                            <  SRR4302545.10367507/94‑1 (MQ=60)
                                                                   CCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTT                            <  SRR4302545.1068107/94‑1 (MQ=60)
                                                                   CCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTT                            <  SRR4302545.85926/94‑1 (MQ=60)
                                                                   CCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTT                            <  SRR4302545.1236044/94‑1 (MQ=60)
                                                                   CCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTT                            >  SRR4302545.2973091/1‑94 (MQ=60)
                                                                   CCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTT                            <  SRR4302545.7407099/94‑1 (MQ=60)
                                                                   CCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTT                            >  SRR4302545.9272666/1‑94 (MQ=60)
                                                                   CCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTT                            <  SRR4302545.10367290/94‑1 (MQ=60)
                                                                     CATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCG                          <  SRR4302545.8708687/94‑1 (MQ=60)
                                                                     CATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCG                          <  SRR4302545.4570665/94‑1 (MQ=60)
                                                                     CATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGACGACCAGCGCACGCCGCCAGAattcc                          >  SRR4302545.6539865/1‑89 (MQ=60)
                                                                      ATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGC                         >  SRR4302545.9098146/1‑94 (MQ=60)
                                                                      ATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGC                         <  SRR4302545.10726430/94‑1 (MQ=60)
                                                                      ATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGC                         >  SRR4302545.10526334/1‑94 (MQ=60)
                                                                      ATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGC                         >  SRR4302545.857962/1‑94 (MQ=60)
                                                                       TTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCC                        >  SRR4302545.453881/1‑94 (MQ=60)
                                                                       TTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCC                        >  SRR4302545.758901/1‑94 (MQ=60)
                                                                       TGTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCC                        <  SRR4302545.526648/94‑1 (MQ=60)
                                                                        TTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCA                       >  SRR4302545.5027806/1‑94 (MQ=60)
                                                                         TGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCAT                      >  SRR4302545.7215090/1‑94 (MQ=60)
                                                                         TGTGCGGCATCTTGCGGCGCGCAGCGGCGTTCACCGCTAATAATCTGCGAACCGTCCGGCGCGCAGACCAGCGCACGCAGCCAGATTTCGCCAT                      <  SRR4302545.10378656/94‑1 (MQ=60)
                                                                          GTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATC                     >  SRR4302545.5601293/1‑94 (MQ=60)
                                                                          GTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATC                     <  SRR4302545.5580746/94‑1 (MQ=60)
                                                                          GTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATC                     >  SRR4302545.1736352/1‑94 (MQ=60)
                                                                          GTTCGGCAGCGGGCGGCGCGCCTCGGTGTTCACCGCGAATAATCTGCGAACTTTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGtcttc                     >  SRR4302545.9617824/1‑89 (MQ=40)
                                                                           TTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCTCACGCAGCCAGATTTCGCCATCA                    >  SRR4302545.6028847/1‑94 (MQ=60)
                                                                            TCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAA                   >  SRR4302545.8209335/1‑94 (MQ=60)
                                                                              GGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATA                 <  SRR4302545.1575159/94‑1 (MQ=60)
                                                                              GGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATA                 <  SRR4302545.10851116/94‑1 (MQ=60)
                                                                              GGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATA                 >  SRR4302545.6368089/1‑94 (MQ=60)
                                                                              GGCATCGTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATA                 >  SRR4302545.10836135/1‑94 (MQ=60)
                                                                               GCCTCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGTGCCGACAAGCGCCCGCAGCCAGATTTCGCCATCAATAA                <  SRR4302545.781802/94‑1 (MQ=60)
                                                                                 CTCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGC              <  SRR4302545.1880183/94‑1 (MQ=60)
                                                                                 ATCTTGCTGCGCGCCGCGGCGTTCACCGCGAATAATATGCGCACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGGCATCAATAAGC              >  SRR4302545.10795147/1‑94 (MQ=60)
                                                                                 ATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGC              >  SRR4302545.613935/1‑94 (MQ=60)
                                                                                 ATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGC              >  SRR4302545.10104045/1‑94 (MQ=60)
                                                                                 ATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGC              <  SRR4302545.938556/94‑1 (MQ=60)
                                                                                 ATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGC              >  SRR4302545.304154/1‑94 (MQ=60)
                                                                                   CTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCCGCCAGATTTCTCCATCAACAAGCTG            >  SRR4302545.4928886/1‑94 (MQ=60)
                                                                                   CTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTC            >  SRR4302545.4928887/1‑94 (MQ=60)
                                                                                   CTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTC            >  SRR4302545.5423702/1‑94 (MQ=60)
                                                                                    TTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCG           <  SRR4302545.1368086/94‑1 (MQ=60)
                                                                                    TTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCG           >  SRR4302545.217150/1‑94 (MQ=60)
                                                                                    TTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCG           >  SRR4302545.2439771/1‑94 (MQ=60)
                                                                                     TGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGG          <  SRR4302545.613562/94‑1 (MQ=60)
                                                                                     TGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGG          <  SRR4302545.2756153/94‑1 (MQ=60)
                                                                                      GCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGTGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGC         <  SRR4302545.3080428/94‑1 (MQ=60)
                                                                                      GCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGC         <  SRR4302545.2369649/94‑1 (MQ=60)
                                                                                       TGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCG        >  SRR4302545.3130106/1‑94 (MQ=60)
                                                                                       CGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCA        <  SRR4302545.9859108/94‑1 (MQ=60)
                                                                                         GCGCGCCGCGGGTTTCCCCGCAAATAATCTGCAAACCGTCCGGCCCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGGGTA      <  SRR4302545.9283857/94‑1 (MQ=60)
                                                                                         GCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTA      <  SRR4302545.3222580/94‑1 (MQ=60)
                                                                                          CGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAG     <  SRR4302545.2179723/94‑1 (MQ=60)
                                                                                           GCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGC    <  SRR4302545.9461096/94‑1 (MQ=60)
                                                                                           GCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGC    <  SRR4302545.7007908/94‑1 (MQ=60)
                                                                                            CGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCT   <  SRR4302545.8821194/94‑1 (MQ=60)
                                                                                            CGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCT   <  SRR4302545.5195118/94‑1 (MQ=60)
                                                                                             GCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTA  >  SRR4302545.378902/1‑94 (MQ=60)
                                                                                             |                                                                                             
TCCGTTATAGACTTCAGCGAGGATCTCGCGCGCGCCGTTATTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCCGGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTA  >  NC_000913/3987453‑3987639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑