Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,997,520 A→G E642G (GAA→GGA)  uvrD → DNA‑dependent ATPase I and helicase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,997,5200AG99.4% 1761.1 / ‑2.2 512E642G (GAA→GGA) uvrDDNA‑dependent ATPase I and helicase II
Reads supporting (aligned to +/- strand):  ref base A (0/3);  new base G (279/230);  total (279/233)
Fisher's exact test for biased strand distribution p-value = 9.36e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 545 total aligned reads displayed.

CTGACCTACGCGGAAACCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGAACGACAGCGGCTACAAGCT  >  NC_000913/3997429‑3997610
                                                                                           |                                                                                          
 tGACCTACGCGGAAACCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGgaga                                                                                         >  2:9717988/1‑94 (MQ=255)
  gACCTACGCGGAAACCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGagag                                                                                        >  1:1138354/1‑94 (MQ=255)
   aCCTACGCGGAAACCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAgg                                                                                       >  2:2100172/1‑94 (MQ=255)
   aCCTACGCGGAAACCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAgg                                                                                       >  2:6292583/1‑94 (MQ=255)
   aCCTACGCGGAAACCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGGGTGTGGGAGAgg                                                                                       >  1:657833/1‑94 (MQ=255)
    ccTGCGCGGAAACCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGt                                                                                      <  2:8908499/94‑1 (MQ=255)
    ccTACGCGGAAACCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGt                                                                                      >  1:7352359/1‑94 (MQ=255)
    ccTACGCGGAAACCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGt                                                                                      <  2:7204750/94‑1 (MQ=255)
     ctgcgcgGAAACCCGCCGTCTGTATGCTAAAGAGGTTTACCATCGCCCGTCGCGCTTTAGCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTg                                                                                     <  1:627659/91‑1 (MQ=255)
     cTACGCGGAAACCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGCGGTg                                                                                     >  2:2413288/1‑94 (MQ=255)
     cTACGCGGAAACCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAgggg                                                                                     >  1:7849366/1‑92 (MQ=255)
     cTACGCGGAAACCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTg                                                                                     <  1:2064842/94‑1 (MQ=255)
     cTACGCGGAAACCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTg                                                                                     >  2:5494939/1‑94 (MQ=255)
     cTACGCGGAAACCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTg                                                                                     <  2:2001677/94‑1 (MQ=255)
     cTACGCGGAAACCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTg                                                                                     <  2:8505383/94‑1 (MQ=255)
       aCGCGGAAACCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTgcg                                                                                   >  1:967344/1‑94 (MQ=255)
        cgcgGAAACCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTgcgc                                                                                  >  2:8787194/1‑94 (MQ=255)
         gcgGAAACCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGcc                                                                                 >  2:3082177/1‑94 (MQ=255)
          cgGAAACCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCt                                                                                >  1:9777168/1‑94 (MQ=255)
           ggAAACCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTg                                                                               >  2:496961/1‑94 (MQ=255)
           ggAAACCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTg                                                                               >  2:4170274/1‑94 (MQ=255)
           ggAAACCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGAGCGCCTg                                                                               >  2:8267924/1‑94 (MQ=255)
               aCCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTgcgcg                                                                           >  2:6812428/1‑94 (MQ=255)
               aCCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTgcgcg                                                                           <  2:4553710/94‑1 (MQ=255)
                    ccgTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACg                                                                      >  2:10479776/1‑94 (MQ=255)
                    ccgTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACg                                                                      >  1:1020904/1‑94 (MQ=255)
                    ccgTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACg                                                                      >  1:1762210/1‑94 (MQ=255)
                    ccgTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACg                                                                      >  1:1042346/1‑94 (MQ=255)
                     cgTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACgg                                                                     <  1:9033087/94‑1 (MQ=255)
                      gTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGTCTGCGCGCCACgga                                                                    >  1:1938811/1‑93 (MQ=255)
                      gTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACgga                                                                    >  2:7047783/1‑93 (MQ=255)
                      gTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGt                                                                    >  2:3381772/1‑94 (MQ=255)
                      gTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGt                                                                    >  2:5241336/1‑94 (MQ=255)
                       tCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTa                                                                   >  2:2275914/1‑94 (MQ=255)
                        cTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTaa                                                                  <  2:10212365/94‑1 (MQ=255)
                           tATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAgcc                                                               <  1:7087692/94‑1 (MQ=255)
                             tGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAgccgc                                                             <  1:4698760/94‑1 (MQ=255)
                               gTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGccc                                                           <  1:1583589/94‑1 (MQ=255)
                                tAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCg                                                          <  2:1668941/94‑1 (MQ=255)
                                 aaaGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCgg                                                         <  1:2428526/94‑1 (MQ=255)
                                 aaaGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCgg                                                         >  1:5186744/1‑94 (MQ=255)
                                  aaGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGt                                                        >  2:4381396/1‑94 (MQ=255)
                                   agagGTTTACCATCGCCCGTCGCACTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTc                                                       <  1:7255853/94‑1 (MQ=255)
                                    gagGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCa                                                      <  1:9558139/94‑1 (MQ=255)
                                     agGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAg                                                     >  2:857543/1‑94 (MQ=255)
                                       gTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGcc                                                   >  1:10103000/1‑94 (MQ=255)
                                        tttACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCa                                                  >  1:3570266/1‑94 (MQ=255)
                                           aCCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCa                                               <  1:7585629/94‑1 (MQ=255)
                                           aCCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCa                                               <  1:4290155/94‑1 (MQ=255)
                                            ccATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGAAAGACGCCCGGTCAGCCATCAg                                              >  2:6689790/1‑94 (MQ=255)
                                            ccATCGCCCGTCGCGCTTTATCGGAGGGCTGCCGGAAGAGTGAGTGGGAGAGGTGAGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAg                                              >  2:6102933/1‑94 (MQ=255)
                                              aTCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCg                                            >  2:9536515/1‑94 (MQ=255)
                                                cGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGa                                          >  2:8394484/1‑94 (MQ=255)
                                                 gCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGAt                                         <  2:2636787/94‑1 (MQ=255)
                                                  cccGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATg                                        >  2:6603137/1‑94 (MQ=255)
                                                   ccGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATgg                                       >  1:2963800/1‑94 (MQ=255)
                                                    cGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATggg                                      >  1:4067283/1‑94 (MQ=255)
                                                     gTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGt                                     <  2:2559050/94‑1 (MQ=255)
                                                      tCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTa                                    <  1:694460/94‑1 (MQ=255)
                                                       tgcgcTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTAc                                   <  2:9757955/93‑1 (MQ=255)
                                                        gcgcTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACg                                  >  1:229096/1‑94 (MQ=255)
                                                          tcTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGTTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGcc                                <  1:6745228/93‑1 (MQ=255)
                                                          gcTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGcc                                <  1:824087/94‑1 (MQ=255)
                                                          gcTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGGACGcc                                >  1:3884406/1‑94 (MQ=255)
                                                           cTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCg                               >  2:5696921/1‑94 (MQ=255)
                                                            tttATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGa                              >  1:7971080/1‑94 (MQ=255)
                                                             ttATCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATAAGCGGATGGGTACGCCGag                             >  1:4519474/1‑93 (MQ=255)
                                                               ttCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATgg                           <  2:3285475/93‑1 (MQ=255)
                                                               aTCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATgg                           >  1:3412377/1‑94 (MQ=255)
                                                                tCGGCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGt                          <  1:9175616/94‑1 (MQ=255)
                                                                  ggCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCg                        <  2:3768320/94‑1 (MQ=255)
                                                                   gCGAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCga                       >  2:10346080/1‑94 (MQ=255)
                                                                     gAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCgaga                     >  2:271742/1‑94 (MQ=255)
                                                                     gAGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCAATCAGCGGCTGGGTACGCCGATGGTCgaga                     >  2:7876289/1‑94 (MQ=255)
                                                                      gggtGCCGGAAGAGTGTGTGGGAGAGGTGCGCCGGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGaa                    <  2:6490573/91‑1 (MQ=255)
                                                                      aGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGaa                    >  2:7582228/1‑94 (MQ=255)
                                                                      aGCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGaa                    <  2:4623610/94‑1 (MQ=255)
                                                                       gCTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGAAc                   <  1:1274009/94‑1 (MQ=255)
                                                                        cTGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGACGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGaagg                  >  2:2934262/1‑92 (MQ=255)
                                                                         tGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGAACGa                 >  2:902149/1‑94 (MQ=255)
                                                                         tGCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGAACGa                 >  2:410253/1‑94 (MQ=255)
                                                                          gCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGAACGAc                >  2:813261/1‑94 (MQ=255)
                                                                          gCCGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGAACGAc                <  1:855404/94‑1 (MQ=255)
                                                                           ccGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGCACGACa               >  1:600465/1‑94 (MQ=255)
                                                                           ccGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGAACGACa               <  1:9137488/94‑1 (MQ=255)
                                                                            cGGTAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGAAGGGCGAGAACGACAg              >  1:9382719/1‑94 (MQ=255)
                                                                            cGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGAACGACAg              >  1:5011000/1‑94 (MQ=255)
                                                                            cGGAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGAACGACAg              <  2:10013356/94‑1 (MQ=255)
                                                                              gAAGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGAACGACAGCg            <  2:1158736/94‑1 (MQ=255)
                                                                               aaGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGAACGACAGCgg           >  2:9373120/1‑94 (MQ=255)
                                                                               aaGAGTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGAACGACAGCgg           <  2:3932449/94‑1 (MQ=255)
                                                                                agagTGTGTGGGATAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGAACGACAGCGGc          <  1:5566887/94‑1 (MQ=255)
                                                                                agagTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGAGCGACAGCGGc          >  1:8901652/1‑94 (MQ=255)
                                                                                agagTGTGTGGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGAACGACAGCGGc          <  1:8824534/94‑1 (MQ=255)
                                                                                agagTGTGTGGGAGAGGAGCGCCTGCGCGCCACGGTAAGACGCCCGGTCAGCCATCAGCGGATGGGGACGCCGATGGTCGAGAACGACAGGGGc          >  2:7407369/1‑94 (MQ=255)
                                                                                    tgtgtgGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGAACGACAGCGGCTACa      <  2:5912040/94‑1 (MQ=255)
                                                                                    tgtgggGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGAACGACAGCGGCTACa      <  1:5118369/94‑1 (MQ=255)
                                                                                    ggtgtgGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGAACGACAGCGGCTACa      <  2:1506259/93‑1 (MQ=255)
                                                                                     gtgtgGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGAACGACAGCGGCTACaa     <  1:139838/94‑1 (MQ=255)
                                                                                      tgtgGGAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGAACGACAGCGGCTACAAg    <  1:6341537/94‑1 (MQ=255)
                                                                                           |                                                                                          
CTGACCTACGCGGAAACCCGCCGTCTGTATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGTGTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGTACGCCGATGGTCGAGAACGACAGCGGCTACAAGCT  >  NC_000913/3997429‑3997610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 368 total aligned reads displayed.
GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑G‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTT  >  NC_000913/3998197‑3998382
                                                                                              |                                                                                             
GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑G                                                                                               <  SRR4302545.1837549/94‑1 (MQ=60)
GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑G                                                                                               <  SRR4302545.7246814/94‑1 (MQ=60)
GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑G                                                                                               <  SRR4302545.5412079/94‑1 (MQ=60)
 ACTGCACCGCAACCTGAATCTCTTTAATGGTTTTGAGGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑G‑A                                                                                             <  SRR4302545.9666356/94‑1 (MQ=60)
 ACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑G‑G                                                                                             <  SRR4302545.3751796/94‑1 (MQ=60)
 ACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑G‑G                                                                                             <  SRR4302545.977529/94‑1 (MQ=60)
 ACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAAAACGCCGCCA‑G‑G                                                                                             >  SRR4302545.3129973/1‑94 (MQ=60)
  CTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑Gga                                                                                             >  SRR4302545.734492/1‑92 (MQ=60)
   TGCACCGCAAAGTGAATTTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑Ggac                                                                                            <  SRR4302545.9510282/94‑4 (MQ=60)
   TGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGCCGAACGCTGCTGACTTTATCTCCCGCTACATAAAACACGCCGCCA‑Ggac                                                                                            <  SRR4302545.6951075/94‑4 (MQ=60)
   TGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCTACATAAAACACGCCGCCA‑Ggac                                                                                            <  SRR4302545.10739433/94‑4 (MQ=60)
   TGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑Ggac                                                                                            >  SRR4302545.2253997/1‑91 (MQ=60)
    GCACCGCACACGGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACG                                                                                           <  SRR4302545.3200512/94‑1 (MQ=60)
    GCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACG                                                                                           <  SRR4302545.3344894/94‑1 (MQ=60)
    GCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACG                                                                                           <  SRR4302545.1350507/94‑1 (MQ=60)
     CACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCATG‑ACGG                                                                                          <  SRR4302545.6988726/94‑1 (MQ=60)
       CCGCAAACTGAATCTCTTTACTGGTTTTGAAGTCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGAT                                                                                        <  SRR4302545.1886281/94‑1 (MQ=60)
        CGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATA                                                                                       >  SRR4302545.2279184/1‑94 (MQ=60)
        CGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATA                                                                                       <  SRR4302545.2827759/94‑1 (MQ=60)
         GCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATCA                                                                                      >  SRR4302545.9632214/1‑94 (MQ=60)
         GCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAA                                                                                      >  SRR4302545.4495070/1‑94 (MQ=60)
           AAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACATGCCTCCAGG‑ACGGATAAAG                                                                                    >  SRR4302545.9646168/1‑94 (MQ=60)
           AAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATATAG                                                                                    >  SRR4302545.7898463/1‑94 (MQ=60)
           AAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAG                                                                                    <  SRR4302545.1246635/94‑1 (MQ=60)
            AACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGA                                                                                   <  SRR4302545.8593325/94‑1 (MQ=60)
            AACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGA                                                                                   <  SRR4302545.2400453/94‑1 (MQ=60)
            AACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGA                                                                                   <  SRR4302545.8431764/94‑1 (MQ=60)
             ACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAA                                                                                  >  SRR4302545.8273472/1‑94 (MQ=60)
              CTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAA                                                                                 >  SRR4302545.9396112/1‑94 (MQ=60)
               TGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAAT                                                                                >  SRR4302545.2769452/1‑94 (MQ=60)
               TGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAAT                                                                                >  SRR4302545.1423176/1‑94 (MQ=60)
               TGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAAT                                                                                >  SRR4302545.4660248/1‑94 (MQ=60)
               TGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAAT                                                                                >  SRR4302545.1664239/1‑94 (MQ=60)
                GAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATT                                                                               >  SRR4302545.1826684/1‑94 (MQ=60)
                 AATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTC                                                                              >  SRR4302545.6901829/1‑94 (MQ=60)
                 AATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTC                                                                              <  SRR4302545.4161731/94‑1 (MQ=60)
                  ATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCC                                                                             >  SRR4302545.9144353/1‑94 (MQ=60)
                   TCTCTTTACTGGTTTTGAATGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCC                                                                            <  SRR4302545.4941069/94‑1 (MQ=60)
                    CTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCT                                                                           <  SRR4302545.9477071/94‑1 (MQ=60)
                    CTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCT                                                                           >  SRR4302545.10925680/1‑94 (MQ=60)
                    CTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCT                                                                           <  SRR4302545.2615191/94‑1 (MQ=60)
                    CTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCT                                                                           >  SRR4302545.8636151/1‑94 (MQ=60)
                     TCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTT                                                                          <  SRR4302545.6125030/94‑1 (MQ=60)
                     TCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTT                                                                          <  SRR4302545.1055584/94‑1 (MQ=60)
                     TCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTT                                                                          >  SRR4302545.8472089/1‑94 (MQ=60)
                      CTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTC                                                                         >  SRR4302545.10592266/1‑94 (MQ=60)
                      CTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTC                                                                         >  SRR4302545.4638564/1‑94 (MQ=60)
                       TTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCA                                                                        <  SRR4302545.2737354/94‑1 (MQ=60)
                        TTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAACCACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCAC                                                                       <  SRR4302545.2334278/94‑1 (MQ=60)
                         TACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACC                                                                      >  SRR4302545.4302796/1‑94 (MQ=60)
                         TACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACC                                                                      >  SRR4302545.2080343/1‑94 (MQ=60)
                          ACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCT                                                                     >  SRR4302545.9158595/1‑94 (MQ=60)
                          ACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCT                                                                     <  SRR4302545.8193272/94‑1 (MQ=60)
                           CTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTG                                                                    <  SRR4302545.2175103/94‑1 (MQ=60)
                           CTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTA                                                                    >  SRR4302545.7497778/1‑94 (MQ=60)
                            TGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGGCATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTCA                                                                   >  SRR4302545.4356685/1‑94 (MQ=60)
                            TGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGA                                                                   >  SRR4302545.4557220/1‑94 (MQ=60)
                            TGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGA                                                                   >  SRR4302545.6223956/1‑94 (MQ=60)
                            TGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGA                                                                   <  SRR4302545.5880076/94‑1 (MQ=60)
                             GGTGTTGAAGGCATCCAGACGAACGATGCCTACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAA                                                                  <  SRR4302545.5142101/94‑1 (MQ=60)
                              GTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAG                                                                 <  SRR4302545.1538408/94‑1 (MQ=60)
                              GTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAG                                                                 >  SRR4302545.283839/1‑94 (MQ=60)
                               TTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGC                                                                <  SRR4302545.4551748/94‑1 (MQ=60)
                               TTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGC                                                                <  SRR4302545.3223015/94‑1 (MQ=60)
                                TTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCG                                                               <  SRR4302545.9701517/94‑1 (MQ=60)
                                 TTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGA                                                              >  SRR4302545.5416878/1‑94 (MQ=60)
                                  TGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAACAACGCCGCCAGG‑ACGGTTAAAGAAATTCCCTTCACCTGAAGCGAG                                                             >  SRR4302545.7374981/1‑94 (MQ=60)
                                  TGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAG                                                             >  SRR4302545.2735036/1‑94 (MQ=60)
                                  TGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAG                                                             >  SRR4302545.6013345/1‑94 (MQ=60)
                                    AAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGAT                                                           <  SRR4302545.7106642/94‑1 (MQ=60)
                                        CATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAA                                                       >  SRR4302545.3107084/1‑94 (MQ=60)
                                        CATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAA                                                       <  SRR4302545.6678104/94‑1 (MQ=60)
                                         ATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAATCACGCCGCCA‑GAACGGATAAAGAAATTCCCTTCCCCTGAAGAGAGATTTAAC                                                      >  SRR4302545.8577202/1‑94 (MQ=60)
                                         ATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAAC                                                      <  SRR4302545.2693272/94‑1 (MQ=60)
                                         ATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAAC                                                      <  SRR4302545.4319188/94‑1 (MQ=60)
                                         ATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAAC                                                      <  SRR4302545.2813885/94‑1 (MQ=60)
                                         ATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAAC                                                      >  SRR4302545.5942105/1‑94 (MQ=60)
                                          TCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACG                                                     >  SRR4302545.10109176/1‑94 (MQ=60)
                                          TCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACG                                                     <  SRR4302545.9246840/94‑1 (MQ=60)
                                            CAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCC                                                   >  SRR4302545.7971833/1‑94 (MQ=60)
                                             AGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCA                                                  >  SRR4302545.4823047/1‑94 (MQ=60)
                                             AGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCA                                                  >  SRR4302545.5164554/1‑94 (MQ=60)
                                             AGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCA                                                  <  SRR4302545.6028984/94‑1 (MQ=60)
                                              GACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCAC                                                 <  SRR4302545.3580186/94‑1 (MQ=60)
                                              GACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCAC                                                 >  SRR4302545.7317771/1‑94 (MQ=60)
                                               ACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACC                                                >  SRR4302545.9927370/1‑94 (MQ=60)
                                                CGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCT                                               >  SRR4302545.6248935/1‑94 (MQ=60)
                                                CGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCT                                               >  SRR4302545.1736257/1‑94 (MQ=60)
                                                CGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCT                                               >  SRR4302545.10885493/1‑94 (MQ=60)
                                                 GAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTT                                              <  SRR4302545.1690098/94‑1 (MQ=60)
                                                 GAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTT                                              >  SRR4302545.7582665/1‑94 (MQ=60)
                                                 GAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTT                                              >  SRR4302545.7652537/1‑94 (MQ=60)
                                                 GAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTT                                              >  SRR4302545.6596289/1‑94 (MQ=60)
                                                 GAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTT                                              >  SRR4302545.6638464/1‑94 (MQ=60)
                                                 GAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTT                                              >  SRR4302545.10698705/1‑94 (MQ=60)
                                                  AACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTC                                             >  SRR4302545.6396300/1‑94 (MQ=60)
                                                  AACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTC                                             >  SRR4302545.5193980/1‑94 (MQ=60)
                                                  AACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTC                                             <  SRR4302545.1215304/94‑1 (MQ=60)
                                                  AACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTC                                             >  SRR4302545.676138/1‑94 (MQ=60)
                                                   ACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCT                                            <  SRR4302545.3369923/94‑1 (MQ=60)
                                                   ACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCT                                            >  SRR4302545.110247/1‑94 (MQ=60)
                                                   ACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCT                                            >  SRR4302545.1368790/1‑94 (MQ=60)
                                                   ACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCT                                            <  SRR4302545.5905986/94‑1 (MQ=60)
                                                   ACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCACTTCACCTGAAGCGAGATTTAACGCCACCTTCT                                            >  SRR4302545.564653/1‑94 (MQ=60)
                                                   ACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGACAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCT                                            <  SRR4302545.1543857/94‑1 (MQ=60)
                                                   ACGATGCCGACTTTACATCCAGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCTAGATTTAACGCCACCTTCT                                            <  SRR4302545.10668370/94‑1 (MQ=60)
                                                    CGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTG                                           >  SRR4302545.4589874/1‑94 (MQ=60)
                                                    CGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTG                                           >  SRR4302545.8345010/1‑94 (MQ=60)
                                                    CGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTG                                           <  SRR4302545.6446778/94‑1 (MQ=60)
                                                    CGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTG                                           >  SRR4302545.2994699/1‑94 (MQ=60)
                                                    CGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTG                                           >  SRR4302545.1183921/1‑94 (MQ=60)
                                                    CGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTC                                           >  SRR4302545.720867/1‑94 (MQ=60)
                                                     GATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTgtgt                                          >  SRR4302545.3506971/1‑90 (MQ=60)
                                                      ATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCT                                         >  SRR4302545.4101416/1‑94 (MQ=60)
                                                      ATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCT                                         >  SRR4302545.446967/1‑94 (MQ=60)
                                                       TGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTG                                        <  SRR4302545.6930825/94‑1 (MQ=60)
                                                       TGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTG                                        >  SRR4302545.3061698/1‑94 (MQ=60)
                                                       TGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTG                                        >  SRR4302545.773367/1‑94 (MQ=60)
                                                        GCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGA                                       >  SRR4302545.9006813/1‑94 (MQ=60)
                                                        GCCGACTTTATCTCCCCCGACATAAAACACGCCGCCATG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGGGATTTAACGCCACCTTCTGCTGG                                       >  SRR4302545.8619354/1‑94 (MQ=60)
                                                         CCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGAC                                      >  SRR4302545.1602980/1‑94 (MQ=60)
                                                         CCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGAC                                      <  SRR4302545.1547080/94‑1 (MQ=60)
                                                         CCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGAC                                      <  SRR4302545.1030601/94‑1 (MQ=60)
                                                          CGACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACC                                     <  SRR4302545.2653748/94‑1 (MQ=60)
                                                           GACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCG                                    <  SRR4302545.831086/94‑1 (MQ=60)
                                                           GACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCG                                    >  SRR4302545.4730905/1‑94 (MQ=60)
                                                           GACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCG                                    >  SRR4302545.2916459/1‑94 (MQ=60)
                                                           GACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCG                                    >  SRR4302545.5951899/1‑94 (MQ=60)
                                                           GACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCG                                    >  SRR4302545.8748248/1‑94 (MQ=60)
                                                           GACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCG                                    <  SRR4302545.4575042/94‑1 (MQ=60)
                                                           GACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCG                                    <  SRR4302545.1166178/94‑1 (MQ=60)
                                                            ACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGT                                   >  SRR4302545.1160039/1‑94 (MQ=60)
                                                            ACTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGT                                   >  SRR4302545.8920572/1‑94 (MQ=60)
                                                             CTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTT                                  >  SRR4302545.5343497/1‑94 (MQ=60)
                                                             CTTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTGAACGCCACCTTCTGCTGACCGTT                                  >  SRR4302545.5988035/1‑94 (MQ=60)
                                                              TTTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTT                                 >  SRR4302545.10283322/1‑94 (MQ=60)
                                                              TTTACCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTT                                 <  SRR4302545.2034794/94‑1 (MQ=60)
                                                               TTATCTCCCGCGCCATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAAATTTAACGCCACCTTCTGCTGACCGTTTT                                <  SRR4302545.2487043/94‑1 (MQ=60)
                                                               TTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTT                                <  SRR4302545.3874757/94‑1 (MQ=60)
                                                               TTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTT                                <  SRR4302545.10231287/94‑1 (MQ=60)
                                                               TTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTT                                <  SRR4302545.915813/94‑1 (MQ=60)
                                                               TTATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTT                                <  SRR4302545.2089581/94‑1 (MQ=60)
                                                                TATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTC                               <  SRR4302545.7644440/94‑1 (MQ=60)
                                                                TATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTC                               <  SRR4302545.1230348/94‑1 (MQ=60)
                                                                TATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTC                               <  SRR4302545.147218/94‑1 (MQ=60)
                                                                TATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTC                               <  SRR4302545.6917315/94‑1 (MQ=60)
                                                                 ATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCG                              >  SRR4302545.8836404/1‑94 (MQ=60)
                                                                 ATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCG                              >  SRR4302545.3631790/1‑94 (MQ=60)
                                                                 ATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCG                              <  SRR4302545.6672616/94‑1 (MQ=60)
                                                                 ATCTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTACG                              >  SRR4302545.10890792/1‑94 (MQ=60)
                                                                  TCTCCCGCTACATAAAACACGCCTCCA‑GCACGGATAAAGAAATTCCCTTCACCAGAAGCGAGATTTAACGCCACCTTCTGCTGACCGCTTTCGA                             <  SRR4302545.8138492/94‑1 (MQ=60)
                                                                   CTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGAT                            >  SRR4302545.6655582/1‑94 (MQ=60)
                                                                   CTCCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGAT                            <  SRR4302545.4840930/94‑1 (MQ=60)
                                                                     CCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGT                          <  SRR4302545.3916097/94‑1 (MQ=60)
                                                                     CCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGT                          >  SRR4302545.10765859/1‑94 (MQ=60)
                                                                     CCCGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGT                          >  SRR4302545.5799682/1‑94 (MQ=60)
                                                                       CGCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTAC                        >  SRR4302545.4074442/1‑94 (MQ=60)
                                                                        GCGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACA                       >  SRR4302545.9037482/1‑94 (MQ=60)
                                                                         CGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAA                      >  SRR4302545.3858680/1‑94 (MQ=60)
                                                                         CGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAA                      >  SRR4302545.1266592/1‑94 (MQ=60)
                                                                         CGACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAA                      <  SRR4302545.4229395/94‑1 (MQ=60)
                                                                          GACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAA                     <  SRR4302545.9488528/94‑1 (MQ=60)
                                                                          GACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAA                     <  SRR4302545.1498511/94‑1 (MQ=60)
                                                                           ACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAC                    >  SRR4302545.4423589/1‑94 (MQ=60)
                                                                           ACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAC                    >  SRR4302545.6203959/1‑94 (MQ=60)
                                                                           ACATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAC                    <  SRR4302545.3800737/94‑1 (MQ=60)
                                                                            CATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACC                   >  SRR4302545.959813/1‑94 (MQ=60)
                                                                             ATAAAACACGGCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCG                  <  SRR4302545.5438088/94‑1 (MQ=60)
                                                                             ATAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGTTGTACAAACCG                  >  SRR4302545.1494838/1‑94 (MQ=60)
                                                                              TAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGA                 >  SRR4302545.5066237/1‑94 (MQ=60)
                                                                              TAAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGA                 <  SRR4302545.9337054/94‑1 (MQ=60)
                                                                               TAAACACGCCACCAGG‑ACGGATAAGGACATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAG                <  SRR4302545.9617473/94‑1 (MQ=60)
                                                                               AAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTATCGATGTACAAACCGAG                >  SRR4302545.1433479/1‑94 (MQ=60)
                                                                               AAAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTGCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAG                >  SRR4302545.6875771/1‑94 (MQ=60)
                                                                               AAAACACGCCGCCAGG‑ACGGATAAAGAAATTCACTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAG                <  SRR4302545.9634146/94‑1 (MQ=60)
                                                                                AAACACGCTGCCAGG‑ACGGATAAAGAAATTCCCGTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGC               <  SRR4302545.7290914/94‑1 (MQ=60)
                                                                                AAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGG               >  SRR4302545.2610976/1‑94 (MQ=60)
                                                                                AAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGC               >  SRR4302545.1708634/1‑94 (MQ=60)
                                                                                AAACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGC               <  SRR4302545.7290913/94‑1 (MQ=60)
                                                                                 ACCACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCG              <  SRR4302545.4686518/94‑1 (MQ=60)
                                                                                 AACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCG              >  SRR4302545.2976079/1‑94 (MQ=60)
                                                                                 AACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCG              <  SRR4302545.10487492/94‑1 (MQ=60)
                                                                                  ACACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGG             <  SRR4302545.8071889/94‑1 (MQ=60)
                                                                                   CACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGC            <  SRR4302545.1858568/94‑1 (MQ=60)
                                                                                    ACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCG           >  SRR4302545.6816131/1‑94 (MQ=60)
                                                                                    ACGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCG           >  SRR4302545.10792008/1‑94 (MQ=60)
                                                                                     CGCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGCACAAACCGAGCGGCGC          >  SRR4302545.2940977/1‑94 (MQ=60)
                                                                                      GCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCA         <  SRR4302545.4378326/94‑1 (MQ=60)
                                                                                      GCCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCA         >  SRR4302545.4423622/1‑94 (MQ=60)
                                                                                       CCGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTAGATGTACAAACCGAGCGGCGCAA        >  SRR4302545.7785118/1‑94 (MQ=60)
                                                                                        CGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATA       <  SRR4302545.4904112/94‑1 (MQ=60)
                                                                                        CGCCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATA       >  SRR4302545.4602224/1‑94 (MQ=60)
                                                                                          CCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGC     <  SRR4302545.5611019/94‑1 (MQ=60)
                                                                                          CCAGG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGC     >  SRR4302545.9801377/1‑94 (MQ=60)
                                                                                           cagG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCT    >  SRR4302545.10250509/4‑94 (MQ=60)
                                                                                            agG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTT   >  SRR4302545.10166453/3‑94 (MQ=60)
                                                                                            agG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTT   >  SRR4302545.230041/3‑94 (MQ=60)
                                                                                            agG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTT   <  SRR4302545.7605123/92‑1 (MQ=60)
                                                                                            agG‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTT   >  SRR4302545.1497357/3‑94 (MQ=60)
                                                                                            G‑G‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTT  <  SRR4302545.1361172/94‑1 (MQ=60)
                                                                                              |                                                                                             
GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑G‑ACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTT  >  NC_000913/3998197‑3998382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑