Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,004,984 +CG coding (1516/1833 nt) recQ → ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,004,9831.G99.6% 1702.9 / ‑5.5 501A505A (GCG→GCC) recQATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base G (259/240);  minor base C (1/1);  total (260/241)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009134,004,9832.C99.8% 1706.8 / ‑6.1 502A505A (GCG→GCC) recQATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base . (1/0);  major base C (258/242);  minor base A (1/0);  total (260/242)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 549 total aligned reads displayed.

TCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCT‑CGC‑‑G‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAAC  >  NC_000913/4004892‑4005061
                                                                                                 ||                                                                              
tCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑g‑‑g                                                                               <  2:6869193/94‑1 (MQ=255)
tCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑g‑‑g                                                                               <  1:10055470/94‑1 (MQ=255)
  cGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑g‑‑gcg                                                                             <  1:531841/94‑3 (MQ=255)
          gATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCctct                                                                       <  2:636234/94‑1 (MQ=255)
           aTTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCtt                                                                      <  2:739982/94‑1 (MQ=255)
            ttCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCttt                                                                     <  2:4809225/94‑1 (MQ=255)
             tCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTg                                                                    <  2:2390106/94‑1 (MQ=255)
             tCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTg                                                                    <  1:1850293/94‑1 (MQ=255)
             tCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTg                                                                    <  1:612517/94‑1 (MQ=255)
             tCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTg                                                                    <  2:5701928/94‑1 (MQ=255)
               cccTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCa                                                                  <  2:3466950/93‑1 (MQ=255)
               aCCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCa                                                                  <  2:6694792/94‑1 (MQ=255)
               aCCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCa                                                                  >  2:8108490/1‑94 (MQ=255)
               aCCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCa                                                                  >  1:7015303/1‑94 (MQ=255)
                ccTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCaa                                                                 >  1:4512115/1‑94 (MQ=255)
                ccTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCaa                                                                 >  1:10449649/1‑94 (MQ=255)
                 cTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAAc                                                                >  2:1644409/1‑94 (MQ=255)
                   cGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACtt                                                              <  1:2147410/94‑1 (MQ=255)
                    ggCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTtg                                                             >  2:654283/1‑94 (MQ=255)
                      ccTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑CGCTGCGC‑‑GGCGAATCCTCTTTGCAACTtgcc                                                           >  1:7579404/1‑94 (MQ=255)
                       cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTtgccg                                                          >  2:3014970/1‑94 (MQ=255)
                       cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTtgccg                                                          >  1:10226548/1‑94 (MQ=255)
                       cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATACTCTTTGCAACTtgccg                                                          >  1:7657029/1‑94 (MQ=255)
                        tGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTtgccgt                                                         >  2:2847867/1‑94 (MQ=255)
                        tGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTtgccgt                                                         <  1:9252467/94‑1 (MQ=255)
                        tGGTGACGCAAAATATTACCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTtgccgt                                                         <  2:9403795/94‑1 (MQ=255)
                        gggTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTtgccgt                                                         <  2:10509186/93‑1 (MQ=255)
                           tGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTtgccgtgcc                                                      >  1:5725730/1‑94 (MQ=255)
                           tGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGACGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTtgcagtgcc                                                      >  1:1928958/1‑94 (MQ=255)
                            gACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTtgccgtgccg                                                     <  1:387999/94‑1 (MQ=255)
                            gACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTtgccgtgccg                                                     >  1:3282370/1‑94 (MQ=255)
                              cGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCcgcg                                                   <  2:5887073/94‑1 (MQ=255)
                               gCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGt                                                  >  2:5422732/1‑94 (MQ=255)
                                cAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑CGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGtt                                                 >  1:4024686/1‑93 (MQ=255)
                                 aaaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTAt                                                >  2:4980969/1‑94 (MQ=255)
                                 aaaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTAt                                                <  1:2590154/94‑1 (MQ=255)
                                  aaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATc                                               <  1:8930776/94‑1 (MQ=255)
                                     tatTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTg                                            <  2:7330529/94‑1 (MQ=255)
                                     tatTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTg                                            >  2:2559681/1‑94 (MQ=255)
                                     tatTGCCCAGCATTCTGCCCTACAACTGACACAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTg                                            >  1:3805643/1‑94 (MQ=255)
                                      atTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTgg                                           <  2:199531/94‑1 (MQ=255)
                                       ttGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgc                                          <  2:3914648/94‑1 (MQ=255)
                                        tGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgcg                                         <  1:7884834/94‑1 (MQ=255)
                                          cccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTCTGCG‑CGG‑‑C‑‑GAATCCTCTTTGCCACTTGCCGTGCCGCGTATCGTGGCGCTc                                      >  1:4778720/1‑94 (MQ=255)
                                          cccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCt                                       <  1:2986603/94‑1 (MQ=255)
                                          cccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCt                                       >  1:6772442/1‑94 (MQ=255)
                                            cAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCa                                     <  2:8642633/94‑1 (MQ=255)
                                            cAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCa                                     <  1:5469873/94‑1 (MQ=255)
                                             aGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCaa                                    <  1:9723410/94‑1 (MQ=255)
                                              gCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCaaa                                   >  2:4141762/1‑94 (MQ=255)
                                               cATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc                                  <  2:5228978/94‑1 (MQ=255)
                                               cATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc                                  <  1:7236582/94‑1 (MQ=255)
                                                aTTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAcc                                 <  1:10273977/94‑1 (MQ=255)
                                                   cTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGTGAATACTCTGTGCAACTTGCCGTGCCGGGTGTCGTGGCGCTGATACCgga                              >  1:5575906/1‑92 (MQ=255)
                                                   cTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaa                              <  2:4615825/94‑1 (MQ=255)
                                                      cccTACAACTGCCAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAACGc                           >  2:2745901/1‑94 (MQ=255)
                                                      cccTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                           >  2:9629646/1‑94 (MQ=255)
                                                      cccTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                           <  1:3620840/94‑1 (MQ=255)
                                                      cccTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                           <  2:8267797/94‑1 (MQ=255)
                                                       ccTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCg                          <  1:4642311/94‑1 (MQ=255)
                                                        cTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                         <  2:10213980/94‑1 (MQ=255)
                                                         tACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAt                        >  2:711658/1‑94 (MQ=255)
                                                         tACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAt                        >  1:6172907/1‑94 (MQ=255)
                                                         tACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAt                        <  2:6097610/94‑1 (MQ=255)
                                                           cAACTGTCAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCCCTCAAACCGAAAGCGATGc                      >  2:1451219/1‑94 (MQ=255)
                                                           cAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGc                      >  1:1257924/1‑94 (MQ=255)
                                                           cAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGc                      >  2:7636917/1‑94 (MQ=255)
                                                           cAACTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGc                      <  1:9390505/94‑1 (MQ=255)
                                                            aaCTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                     >  1:9559962/1‑94 (MQ=255)
                                                            aaCTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                     >  1:8061079/1‑94 (MQ=255)
                                                            aaCTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                     <  1:413595/94‑1 (MQ=255)
                                                            aaCTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                     <  1:2277686/94‑1 (MQ=255)
                                                            aaCTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                     <  2:8843281/94‑1 (MQ=255)
                                                             aCTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCTATGCAg                    <  1:5858430/94‑1 (MQ=255)
                                                             aCTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAg                    <  1:1421003/94‑1 (MQ=255)
                                                              cTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGa                   >  1:2855949/1‑94 (MQ=255)
                                                              cTGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGCATCGTGGCGCTCAAACCGAAAGCGATGCAGa                   >  2:3345208/1‑94 (MQ=255)
                                                               tGACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaa                  >  2:1241011/1‑94 (MQ=255)
                                                                gACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaaa                 <  2:8725704/94‑1 (MQ=255)
                                                                gACAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaaa                 >  1:6422760/1‑94 (MQ=255)
                                                                 aCAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAt                <  2:9751621/94‑1 (MQ=255)
                                                                 aCAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAt                <  2:8448905/94‑1 (MQ=255)
                                                                 aCAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAt                >  2:4560208/1‑94 (MQ=255)
                                                                  cAGAGGCCGCGCGCCCGG‑‑TGCTGCGCGGG‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATc               <  2:3689752/94‑1 (MQ=255)
                                                                  cAGAGGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATc               <  1:8524088/94‑1 (MQ=255)
                                                                   agagGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCg              <  2:6449934/94‑1 (MQ=255)
                                                                   agagGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCg              <  1:8615792/94‑1 (MQ=255)
                                                                    gagGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGt             <  2:915705/94‑1 (MQ=255)
                                                                    gagGCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATAGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGt             >  2:10343145/1‑94 (MQ=255)
                                                                      ggCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTc           <  1:4889939/94‑1 (MQ=255)
                                                                       gCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcg          <  2:3076687/94‑1 (MQ=255)
                                                                       gCCGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcg          <  2:9560435/94‑1 (MQ=255)
                                                                        ccGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcgg         <  1:3533766/94‑1 (MQ=255)
                                                                        ccGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcgg         <  1:9173536/94‑1 (MQ=255)
                                                                        ccGCGCGCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcgg         >  1:5099356/1‑94 (MQ=255)
                                                                         cgcgcgCCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggg        >  2:7833428/1‑93 (MQ=255)
                                                                          gcgcgcCCGG‑‑TGCTGCGCT‑GC‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggcg       <  2:441729/94‑1 (MQ=255)
                                                                          gcgcgcCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggcg       <  2:10018647/94‑1 (MQ=255)
                                                                          gcgcgcCCGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggcg       <  1:1671468/94‑1 (MQ=255)
                                                                               cccGG‑‑TGCTGCGC‑‑GGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAAc  >  2:2163300/1‑94 (MQ=255)
                                                                                                 ||                                                                              
TCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGG‑‑TGCT‑CGC‑‑G‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAAC  >  NC_000913/4004892‑4005061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 334 total aligned reads displayed.
CGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑G‑CTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAA  >  NC_000913/4005508‑4005694
                                                                                                |                                                                                             
CGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑G‑C                                                                                               >  SRR4302545.362989/1‑94 (MQ=60)
 GTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑G‑CC                                                                                              >  SRR4302545.4831662/1‑94 (MQ=60)
 GTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑G‑CC                                                                                              >  SRR4302545.4634652/1‑94 (MQ=60)
 GTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑G‑CC                                                                                              >  SRR4302545.6447092/1‑94 (MQ=60)
 GTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑G‑CC                                                                                              <  SRR4302545.4471585/94‑1 (MQ=60)
 GTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑G‑CC                                                                                              <  SRR4302545.789367/94‑1 (MQ=60)
  TAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑G‑Cct                                                                                             >  SRR4302545.2050361/1‑92 (MQ=60)
  TAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑G‑Cct                                                                                             <  SRR4302545.9415740/94‑3 (MQ=60)
   AATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑G‑Cctg                                                                                            >  SRR4302545.8686975/1‑91 (MQ=60)
   AATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑G‑Cctg                                                                                            >  SRR4302545.6613602/1‑91 (MQ=60)
    ATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGT                                                                                            >  SRR4302545.5614289/1‑94 (MQ=60)
       GTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATC                                                                                         <  SRR4302545.2159964/94‑1 (MQ=60)
       GTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATC                                                                                         >  SRR4302545.6165012/1‑94 (MQ=60)
       GTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATC                                                                                         <  SRR4302545.8124298/94‑1 (MQ=60)
       GTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATC                                                                                         >  SRR4302545.7199333/1‑94 (MQ=60)
       GTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATC                                                                                         >  SRR4302545.3793121/1‑94 (MQ=60)
        TTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCT                                                                                        <  SRR4302545.40903/94‑1 (MQ=60)
        TTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCT                                                                                        <  SRR4302545.3206340/94‑1 (MQ=60)
        TTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCT                                                                                        <  SRR4302545.3191257/94‑1 (MQ=60)
        TTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCT                                                                                        <  SRR4302545.756024/94‑1 (MQ=60)
         TTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGGG‑GCCTGTATCT                                                                                        >  SRR4302545.8006432/1‑94 (MQ=60)
         TTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTC                                                                                       >  SRR4302545.7316164/1‑94 (MQ=60)
         TTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGGGGGCGGTG‑GCCTGTATCTC                                                                                       >  SRR4302545.8006430/1‑94 (MQ=60)
          TGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCT                                                                                      <  SRR4302545.10487702/94‑1 (MQ=60)
          TGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCT                                                                                      >  SRR4302545.7434011/1‑94 (MQ=60)
           GGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTG                                                                                     <  SRR4302545.10050801/94‑1 (MQ=60)
           GGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTG                                                                                     >  SRR4302545.10546957/1‑94 (MQ=60)
            GGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGC                                                                                    >  SRR4302545.10115230/1‑94 (MQ=60)
             GCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCT                                                                                   <  SRR4302545.2124978/94‑1 (MQ=60)
             GCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCT                                                                                   <  SRR4302545.807745/94‑1 (MQ=60)
              CTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGGCGTG‑GCCTGGATCTCTGCTG                                                                                  >  SRR4302545.5225182/1‑94 (MQ=60)
              CTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTG                                                                                  <  SRR4302545.3720608/94‑1 (MQ=60)
               gtggtgtttggttgtgtgggttgtgtttgggggggggggggATGGCCTCGCTGCATACGCTGATTATGGTGGGGGGTG‑GCCTGTATCTCTGCTG                                                                                  <  SRR4302545.3118124/53‑1 (MQ=42)
               TGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGG                                                                                 <  SRR4302545.6349280/94‑1 (MQ=60)
                GGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGA                                                                                >  SRR4302545.6815127/1‑94 (MQ=60)
                 GGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGAT                                                                               <  SRR4302545.4742025/94‑1 (MQ=60)
                 GGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGAT                                                                               <  SRR4302545.10772575/94‑1 (MQ=60)
                 GGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGAT                                                                               >  SRR4302545.5480749/1‑94 (MQ=60)
                  GATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATG                                                                              >  SRR4302545.3832927/1‑94 (MQ=60)
                   ATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGG                                                                             <  SRR4302545.2106705/94‑1 (MQ=60)
                     TGCGCTGCTTGGCCTGCATTGGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGT                                                                           <  SRR4302545.9367702/94‑1 (MQ=60)
                      GCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTT                                                                          >  SRR4302545.7120436/1‑94 (MQ=60)
                       CGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTA                                                                         >  SRR4302545.4788133/1‑94 (MQ=60)
                       CGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTA                                                                         <  SRR4302545.8158463/94‑1 (MQ=60)
                       CGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTA                                                                         >  SRR4302545.1431680/1‑94 (MQ=60)
                       CGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGGTA                                                                         >  SRR4302545.5438987/1‑94 (MQ=60)
                        GCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTAC                                                                        >  SRR4302545.10767741/1‑94 (MQ=60)
                         CTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACC                                                                       <  SRR4302545.8413905/94‑1 (MQ=60)
                         CTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACC                                                                       <  SRR4302545.8413906/94‑1 (MQ=60)
                          TGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCA                                                                      <  SRR4302545.2245495/94‑1 (MQ=60)
                          TGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCA                                                                      <  SRR4302545.4960869/94‑1 (MQ=60)
                          TGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTAGCTGCCTACGCTGATTATGGTGGGCGGTG‑GCCTGTTTCTCTGCTGGATGGGTTACCA                                                                      >  SRR4302545.4169262/1‑94 (MQ=60)
                           GCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAG                                                                     <  SRR4302545.5113431/94‑1 (MQ=60)
                           GCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAG                                                                     <  SRR4302545.5602429/94‑1 (MQ=60)
                            CTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGCC‑CTGTATCTCTGCTGGATGGGTTACCAGA                                                                    <  SRR4302545.2809070/94‑1 (MQ=60)
                            CTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGA                                                                    <  SRR4302545.9478360/94‑1 (MQ=60)
                            CTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGA                                                                    <  SRR4302545.9478361/94‑1 (MQ=60)
                            CTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGA                                                                    <  SRR4302545.403242/94‑1 (MQ=60)
                             TTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGAT                                                                   <  SRR4302545.520786/94‑1 (MQ=60)
                             TTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGAT                                                                   <  SRR4302545.9627493/94‑1 (MQ=60)
                               GGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGC                                                                 <  SRR4302545.9566352/94‑1 (MQ=60)
                               GGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGC                                                                 >  SRR4302545.448838/1‑94 (MQ=60)
                                GCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCT                                                                >  SRR4302545.7616433/1‑94 (MQ=60)
                                GCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCT                                                                <  SRR4302545.4548256/94‑1 (MQ=60)
                                GCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCT                                                                >  SRR4302545.8861497/1‑94 (MQ=60)
                                GCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCT                                                                <  SRR4302545.8265224/94‑1 (MQ=60)
                                 CCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGCTTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTA                                                               >  SRR4302545.1683267/1‑94 (MQ=60)
                                 CCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTA                                                               <  SRR4302545.2224918/94‑1 (MQ=60)
                                 CCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGGTACCAGATGCTA                                                               >  SRR4302545.5216846/1‑94 (MQ=60)
                                  CTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTAC                                                              >  SRR4302545.4684463/1‑94 (MQ=60)
                                  CTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGACGCTAC                                                              >  SRR4302545.3349083/1‑94 (MQ=60)
                                  CTGCATTTGATTATCG‑AAAAAATGGCCTCGCTGCATACGCTGATTATGGTGGGCGGAG‑GCCTGTATCTCTGCTGGTTGGGTTACCAGATGCTAC                                                              >  SRR4302545.3266994/1‑94 (MQ=60)
                                     CATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGCTGCTAGGTG                                                           >  SRR4302545.6983096/1‑94 (MQ=60)
                                      ATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGG                                                          >  SRR4302545.7305759/1‑94 (MQ=60)
                                      ATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGG                                                          <  SRR4302545.654377/94‑1 (MQ=60)
                                      ATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGG                                                          <  SRR4302545.6489608/94‑1 (MQ=60)
                                      ATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGG                                                          >  SRR4302545.8644502/1‑94 (MQ=60)
                                        TTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTG                                                        >  SRR4302545.9637160/1‑94 (MQ=60)
                                        TTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTG                                                        <  SRR4302545.517477/94‑1 (MQ=60)
                                        TTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTG                                                        <  SRR4302545.2086188/94‑1 (MQ=60)
                                         TGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGC                                                       >  SRR4302545.2558137/1‑94 (MQ=60)
                                         TGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGC                                                       >  SRR4302545.1308429/1‑94 (MQ=60)
                                          GATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGggcc                                                      >  SRR4302545.2707453/1‑90 (MQ=60)
                                          GATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCA                                                      >  SRR4302545.8610568/1‑94 (MQ=60)
                                          GATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCA                                                      <  SRR4302545.9035773/94‑1 (MQ=60)
                                          GATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCA                                                      >  SRR4302545.9858106/1‑94 (MQ=60)
                                           ATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCAC                                                     >  SRR4302545.2892007/1‑94 (MQ=60)
                                            TTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACT                                                    <  SRR4302545.3546959/94‑1 (MQ=60)
                                            TTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACT                                                    >  SRR4302545.4879563/1‑94 (MQ=60)
                                             TATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTG                                                   >  SRR4302545.635215/1‑94 (MQ=60)
                                             TATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTG                                                   <  SRR4302545.7723231/94‑1 (MQ=60)
                                             TATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTG                                                   >  SRR4302545.5722261/1‑94 (MQ=60)
                                             TATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTG                                                   >  SRR4302545.5934017/1‑94 (MQ=60)
                                             TATCG‑AAAAAATGGCCTGGCTGCATACGCTGAGTATGGTGGGCGGTG‑GCCCGTATCTCTGCTGGATGGGGTACCAGATGCTCCGTGGTGCACTG                                                   >  SRR4302545.6041022/1‑94 (MQ=60)
                                              ATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGA                                                  >  SRR4302545.6858004/1‑94 (MQ=60)
                                               TCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAA                                                 <  SRR4302545.1347203/94‑1 (MQ=60)
                                                CG‑AAAAAATGGCCTGGCTGCCTACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGACA                                                >  SRR4302545.1131342/1‑94 (MQ=60)
                                                CG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAA                                                <  SRR4302545.3342667/94‑1 (MQ=60)
                                                CG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAA                                                >  SRR4302545.5509175/1‑94 (MQ=60)
                                                 G‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAA                                               <  SRR4302545.3811629/94‑1 (MQ=60)
                                                 G‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAA                                               >  SRR4302545.3499347/1‑94 (MQ=60)
                                                    AAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAA                                             >  SRR4302545.4281920/1‑94 (MQ=60)
                                                    AAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAA                                             >  SRR4302545.5387548/1‑94 (MQ=60)
                                                    AAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAA                                             >  SRR4302545.3078357/1‑94 (MQ=60)
                                                       AATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAG                                          >  SRR4302545.8824450/1‑94 (MQ=60)
                                                       AATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAG                                          <  SRR4302545.9037762/94‑1 (MQ=60)
                                                       AATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAG                                          <  SRR4302545.9864894/94‑1 (MQ=60)
                                                        ATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGG                                         >  SRR4302545.8008024/1‑94 (MQ=60)
                                                        ATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGG                                         >  SRR4302545.6523753/1‑94 (MQ=60)
                                                        ATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGG                                         >  SRR4302545.7243848/1‑94 (MQ=60)
                                                        ATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGG                                         <  SRR4302545.3182990/94‑1 (MQ=60)
                                                        ATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGG                                         >  SRR4302545.4556191/1‑94 (MQ=60)
                                                         TGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGC                                        <  SRR4302545.10216806/94‑1 (MQ=60)
                                                         TGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGC                                        <  SRR4302545.9272130/94‑1 (MQ=60)
                                                          GGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCG                                       <  SRR4302545.3223859/94‑1 (MQ=60)
                                                           GCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGG                                      <  SRR4302545.5990829/94‑1 (MQ=60)
                                                             CTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTT                                    <  SRR4302545.4459427/94‑1 (MQ=60)
                                                             CTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTT                                    >  SRR4302545.7083886/1‑94 (MQ=60)
                                                             CTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTT                                    <  SRR4302545.1014637/94‑1 (MQ=60)
                                                             CTGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGGT                                    >  SRR4302545.2127639/1‑94 (MQ=60)
                                                              TGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTT                                   <  SRR4302545.3882660/94‑1 (MQ=60)
                                                              TGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTT                                   <  SRR4302545.7963290/94‑1 (MQ=60)
                                                              TGGCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTT                                   >  SRR4302545.4856128/1‑94 (MQ=60)
                                                              TGGCTGCATACGCGGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTT                                   <  SRR4302545.7454643/94‑1 (MQ=60)
                                                                GCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCT                                 <  SRR4302545.10384923/94‑1 (MQ=60)
                                                                GCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCT                                 >  SRR4302545.7857429/1‑94 (MQ=60)
                                                                GCTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCT                                 <  SRR4302545.1680168/94‑1 (MQ=60)
                                                                 CTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGTACTGAAAAAAGAGGCGGTTTCTG                                >  SRR4302545.7465482/1‑94 (MQ=60)
                                                                 CTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTG                                >  SRR4302545.3227813/1‑94 (MQ=60)
                                                                 CTGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTG                                >  SRR4302545.2662527/1‑94 (MQ=60)
                                                                  TGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGC                               >  SRR4302545.10592333/1‑94 (MQ=60)
                                                                  TGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGC                               <  SRR4302545.8772741/94‑1 (MQ=60)
                                                                  TGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGC                               >  SRR4302545.238995/1‑94 (MQ=60)
                                                                  TGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGC                               >  SRR4302545.3012978/1‑94 (MQ=60)
                                                                  TGCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGC                               >  SRR4302545.4234812/1‑94 (MQ=60)
                                                                   GCATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCA                              >  SRR4302545.3472013/1‑94 (MQ=60)
                                                                    CATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCAC                             >  SRR4302545.1391579/1‑94 (MQ=60)
                                                                    CATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCAC                             >  SRR4302545.3561168/1‑94 (MQ=60)
                                                                     ATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACC                            >  SRR4302545.4606758/1‑94 (MQ=60)
                                                                     ATACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACC                            >  SRR4302545.10852663/1‑94 (MQ=60)
                                                                      TACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCT                           <  SRR4302545.672382/94‑1 (MQ=60)
                                                                      TACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCT                           <  SRR4302545.3051397/94‑1 (MQ=60)
                                                                      TACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCT                           >  SRR4302545.811995/1‑94 (MQ=60)
                                                                       ACGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTG                          <  SRR4302545.4379562/94‑1 (MQ=60)
                                                                        CGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGC                         >  SRR4302545.7055106/1‑94 (MQ=60)
                                                                        CGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGC                         >  SRR4302545.8578158/1‑94 (MQ=60)
                                                                        CGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGC                         <  SRR4302545.10930003/94‑1 (MQ=60)
                                                                        CGCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGC                         >  SRR4302545.9826741/1‑94 (MQ=60)
                                                                         GCTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCG                        >  SRR4302545.9960960/1‑94 (MQ=60)
                                                                          CTGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGCGGCGGTTTCTGGACCTGCGC                       >  SRR4302545.6048406/1‑94 (MQ=60)
                                                                           TGATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCC                      >  SRR4302545.7150744/1‑94 (MQ=60)
                                                                            GATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCA                     <  SRR4302545.3898924/94‑1 (MQ=60)
                                                                            GATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCA                     >  SRR4302545.8501090/1‑94 (MQ=60)
                                                                            GATTATGGTGGGCGGTG‑GCCTGATTCTCTGCTGGATGGGTTACCGGATGCTACGTGGTGCACTGAAAAAGGAGGCGGTTTCTGCACCTCAGCCA                     >  SRR4302545.5400251/1‑94 (MQ=60)
                                                                             ATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCAC                    >  SRR4302545.4078189/1‑94 (MQ=60)
                                                                             ATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCAC                    <  SRR4302545.8205934/94‑1 (MQ=60)
                                                                             ATTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCAC                    <  SRR4302545.8205935/94‑1 (MQ=60)
                                                                              TTATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACA                   >  SRR4302545.10035370/1‑94 (MQ=60)
                                                                               TATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAG                  <  SRR4302545.7652705/94‑1 (MQ=60)
                                                                               TATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAG                  >  SRR4302545.1593171/1‑94 (MQ=60)
                                                                               TATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAG                  >  SRR4302545.9269753/1‑94 (MQ=60)
                                                                               TATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAG                  <  SRR4302545.4701045/94‑1 (MQ=60)
                                                                               TATGGTGGGCGCTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCGCCTGCGCCCCAG                  <  SRR4302545.945054/94‑1 (MQ=60)
                                                                                ATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGG                 >  SRR4302545.1454818/1‑94 (MQ=60)
                                                                                ATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGG                 <  SRR4302545.6954544/94‑1 (MQ=60)
                                                                                ATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGG                 >  SRR4302545.4066520/1‑94 (MQ=60)
                                                                                ATGGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGG                 >  SRR4302545.472585/1‑94 (MQ=60)
                                                                                  GGTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTC               <  SRR4302545.512552/94‑1 (MQ=60)
                                                                                   GTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCG              <  SRR4302545.8582050/94‑1 (MQ=60)
                                                                                   GTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCG              <  SRR4302545.5244877/94‑1 (MQ=60)
                                                                                   GTGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCG              >  SRR4302545.2886934/1‑94 (MQ=60)
                                                                                    TTGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGA             >  SRR4302545.6647856/1‑94 (MQ=60)
                                                                                    TGGTCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACTTGGTGCACTGAAAAAAGAGCCGGTTTCTGCgccttgcccacacgtaga             <  SRR4302545.7418874/94‑19 (MQ=60)
                                                                                    TGGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGA             <  SRR4302545.888494/94‑1 (MQ=60)
                                                                                     GGGCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAG            <  SRR4302545.1934549/94‑1 (MQ=60)
                                                                                       GCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCT          <  SRR4302545.10918937/94‑1 (MQ=60)
                                                                                       GCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCT          <  SRR4302545.8846106/94‑1 (MQ=60)
                                                                                       GCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCT          <  SRR4302545.6634755/94‑1 (MQ=60)
                                                                                       GCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCT          <  SRR4302545.9716600/94‑1 (MQ=60)
                                                                                       GCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCT          <  SRR4302545.9716601/94‑1 (MQ=60)
                                                                                       GCGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCT          <  SRR4302545.5977420/94‑1 (MQ=60)
                                                                                        CGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTG         >  SRR4302545.9897573/1‑94 (MQ=60)
                                                                                        CGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTG         <  SRR4302545.892713/94‑1 (MQ=60)
                                                                                        CGGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTG         >  SRR4302545.5331990/1‑94 (MQ=60)
                                                                                        CGGTG‑GCCAGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTG         >  SRR4302545.4841395/1‑94 (MQ=60)
                                                                                         GGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGG        >  SRR4302545.9262033/1‑94 (MQ=60)
                                                                                         GGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGG        <  SRR4302545.3334151/94‑1 (MQ=60)
                                                                                         GGTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGG        <  SRR4302545.5351319/94‑1 (MQ=60)
                                                                                          GTG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGC       <  SRR4302545.5573075/94‑1 (MQ=60)
                                                                                           ggggcCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGGGGTGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGC       <  SRR4302545.9414301/89‑1 (MQ=60)
                                                                                           TG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCG      <  SRR4302545.3986767/94‑1 (MQ=60)
                                                                                           TG‑GCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCG      <  SRR4302545.2206920/94‑1 (MQ=60)
                                                                                             ggcCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGA     <  SRR4302545.4039544/91‑1 (MQ=60)
                                                                                             ggcCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGA     >  SRR4302545.5492164/4‑94 (MQ=60)
                                                                                              C‑CTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGGCGAGCTGGCGAAA   >  SRR4302545.2321170/1‑94 (MQ=60)
                                                                                              C‑CTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGGTTCTGCACCTGCGCCACAGGTCGAGCTCGCGAAA   >  SRR4302545.9705343/1‑94 (MQ=60)
                                                                                                CTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAA  >  SRR4302545.7755352/1‑94 (MQ=60)
                                                                                                CTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAA  <  SRR4302545.6751074/94‑1 (MQ=60)
                                                                                                CTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAA  <  SRR4302545.9195083/94‑1 (MQ=60)
                                                                                                CTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAA  >  SRR4302545.4459358/1‑94 (MQ=60)
                                                                                                |                                                                                             
CGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCG‑AAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTG‑G‑CTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAA  >  NC_000913/4005508‑4005694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑