Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,008,411 +C coding (494/516 nt) b4555 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,008,4101.C99.6% 833.5 / ‑6.3 240A165P (GCT→CCT) b4555hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base C (116/123);  minor base A (1/0);  total (117/123)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 242 total aligned reads displayed.

GTCTGGAAGTGATGGCGGGCGGCGTTTC‑AAAGGCCATGCGCTGGAAG‑GCTGGCG‑‑‑‑AACT‑GGCTACAG‑CTGAAGGATT‑G‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGAAA‑GC‑TGCATTATGGGCAGTGGCC  >  NC_000913/4008345‑4008493
                                                                         |                                                                                          
gTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGtt                                                                       >  1:7797595/1‑94 (MQ=255)
gTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGtt                                                                       <  1:4516742/94‑1 (MQ=255)
gTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGtt                                                                       <  2:6030018/94‑1 (MQ=255)
 tCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGttt                                                                      <  1:5051237/94‑2 (MQ=255)
 tCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGttt                                                                      >  1:790202/1‑93 (MQ=255)
  cTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGtttg                                                                     <  1:8584841/94‑3 (MQ=255)
  cTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGtttg                                                                     <  2:7792987/94‑3 (MQ=255)
  cTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGtttg                                                                     <  2:5695884/94‑3 (MQ=255)
  cTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGtttg                                                                     <  2:3159666/94‑3 (MQ=255)
   tGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGtttgg                                                                    <  1:9467560/94‑4 (MQ=255)
    ggAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGtttggt                                                                   <  2:4317332/94‑5 (MQ=255)
    ggAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGtttggt                                                                   <  2:10016862/94‑5 (MQ=255)
    ggAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGtttggt                                                                   <  2:8511693/94‑5 (MQ=255)
          gATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACggg                                                             <  1:1431356/94‑1 (MQ=255)
          gATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACggg                                                             <  1:1219882/94‑1 (MQ=255)
          gATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACggg                                                             <  2:6576245/94‑1 (MQ=255)
          gATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACggg                                                             >  1:10380994/1‑94 (MQ=255)
          gATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACggg                                                             <  1:10280941/94‑1 (MQ=255)
            tGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGag                                                           >  2:1347562/1‑93 (MQ=255)
            tGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGag                                                           >  2:716483/1‑93 (MQ=255)
            tGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGAt                                                           <  1:10271385/94‑1 (MQ=255)
            tGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGAt                                                           >  2:2192750/1‑94 (MQ=255)
             ggcgggcgGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATg                                                          >  2:7409542/1‑94 (MQ=255)
             ggcgggcgGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATg                                                          <  1:373493/94‑1 (MQ=255)
              gcgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGa                                                         >  1:7225388/1‑94 (MQ=255)
              gcgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGa                                                         <  2:4537753/94‑1 (MQ=255)
               cgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGaa                                                        >  2:8736980/1‑94 (MQ=255)
               cgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGaa                                                        <  2:2803961/94‑1 (MQ=255)
                gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGaag                                                       >  2:3717254/1‑93 (MQ=255)
                gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAac                                                       >  1:5008300/1‑94 (MQ=255)
                gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAac                                                       <  2:1996423/94‑1 (MQ=255)
                gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAac                                                       <  2:5461396/94‑1 (MQ=255)
                gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAac                                                       <  1:1928675/94‑1 (MQ=255)
                gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAac                                                       <  1:6153311/94‑1 (MQ=255)
                 ggcggcGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacg                                                      <  2:799367/94‑1 (MQ=255)
                 ggcggcGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacg                                                      >  2:9822787/1‑94 (MQ=255)
                  gcggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacga                                                     >  1:2160045/1‑94 (MQ=255)
                  gcggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacga                                                     <  1:9298965/94‑1 (MQ=255)
                  gcggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacga                                                     >  2:6573983/1‑94 (MQ=255)
                   gggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacgac                                                    <  2:2430425/93‑1 (MQ=255)
                   cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGTacgac                                                    >  1:4542112/1‑94 (MQ=255)
                   cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacgac                                                    <  1:8710398/94‑1 (MQ=255)
                   cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTCCGTTTGGTGACGGGATGAaagac                                                    >  2:8629716/1‑94 (MQ=255)
                    ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacgacg                                                   <  2:4709098/94‑1 (MQ=255)
                    ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacgacg                                                   >  1:4820196/1‑94 (MQ=255)
                    ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacgacg                                                   >  1:3482095/1‑94 (MQ=255)
                    ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacgacg                                                   >  1:4606483/1‑94 (MQ=255)
                    ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacgacg                                                   >  1:4117323/1‑94 (MQ=255)
                     gcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGc                                                  <  2:10174737/94‑1 (MQ=255)
                     gcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGc                                                  <  2:3943776/94‑1 (MQ=255)
                      cgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGcc                                                 >  2:9058941/1‑94 (MQ=255)
                      cgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGcc                                                 <  2:4805074/94‑1 (MQ=255)
                      cgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGcc                                                 <  1:5342983/94‑1 (MQ=255)
                       gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGG‑ATTGCGTTTGGTGACGGGATGAACGACCCCg                                                >  2:4874565/1‑94 (MQ=255)
                       gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCg                                                <  2:4971713/94‑1 (MQ=255)
                       gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCg                                                >  2:3693511/1‑94 (MQ=255)
                       gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCg                                                >  1:7543234/1‑94 (MQ=255)
                         ttcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGCACGACGCCCaa                                              >  1:2731701/5‑94 (MQ=255)
                         ttcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGaa                                              <  2:3465770/90‑1 (MQ=255)
                         ttcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGaa                                              <  2:4195817/90‑1 (MQ=255)
                          tcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGaaa                                             <  2:4897641/91‑1 (MQ=255)
                          tcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGaaa                                             >  2:4588664/4‑94 (MQ=255)
                           caaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAt                                            <  1:8337776/92‑1 (MQ=255)
                           caaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAt                                            >  2:1724717/3‑94 (MQ=255)
                           caaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAt                                            <  1:2514554/92‑1 (MQ=255)
                           caaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAt                                            <  1:8198576/92‑1 (MQ=255)
                           caaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAt                                            <  2:8968878/92‑1 (MQ=255)
                           caaaaGGCCATGCGCTGGAAGCGGAGGCGAAGAAACTGGGCTACAGCCTGAAGGGTT‑GTATTGCGTTTGGTGACGGGATGAACGACGTCGaact                                            >  1:1501248/3‑92 (MQ=255)
                            aaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATg                                           >  1:5472649/2‑94 (MQ=255)
                            aaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATg                                           <  2:705039/93‑1 (MQ=255)
                            aaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATg                                           >  1:4787698/2‑94 (MQ=255)
                             aaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGc                                          <  1:1012806/94‑1 (MQ=255)
                               aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTg                                        >  2:1637322/1‑94 (MQ=255)
                               aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTg                                        <  1:10413929/94‑1 (MQ=255)
                               aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTg                                        >  2:295532/1‑94 (MQ=255)
                               aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTg                                        <  2:2778905/94‑1 (MQ=255)
                                ggCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGt                                       <  2:398554/94‑1 (MQ=255)
                                ggCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGt                                       >  1:3013830/1‑94 (MQ=255)
                                                             actgggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGC‑TGCATTATGGGc         <  1:6282792/90‑1 (MQ=255)
                                                              ctgggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGC‑TGCATTATGGGCa        >  2:5255261/4‑94 (MQ=255)
                                                              ctgggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGC‑TGCATTATGGGCa        <  2:5316245/91‑1 (MQ=255)
                                                              ctgggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGC‑TGCATTATGGGCa        <  2:4995888/91‑1 (MQ=255)
                                                              ctgggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGC‑TGCATTATGGGCa        >  1:7767463/4‑94 (MQ=255)
                                                              ctgggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGC‑TGCATTATGGGCa        >  2:4390419/4‑94 (MQ=255)
                                                              ctgggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGC‑TGCATTATGGGCa        <  2:7030967/91‑1 (MQ=255)
                                                              ctgggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGC‑TGCATTATGGGCa        >  2:2657698/4‑94 (MQ=255)
                                                               tgggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGC‑TGCATTATGGGCAg       <  2:9895128/92‑1 (MQ=255)
                                                               tgggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGC‑TGCATTATGGGCAg       >  2:7078220/3‑94 (MQ=255)
                                                               tgggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGC‑TGCATTATGGGCAg       >  2:591943/3‑94 (MQ=255)
                                                                gggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGC‑TGCATTATGGGCAGt      >  2:818008/2‑94 (MQ=255)
                                                                gggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGC‑TGCATTATGGGCAGt      >  2:4779606/2‑94 (MQ=255)
                                                                gggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGC‑TGCATTATGGGCAGt      <  2:10542244/93‑1 (MQ=255)
                                                                 ggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGC‑TGCATTATGGGCAGTg     >  2:6170472/1‑94 (MQ=255)
                                                                 ggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGC‑TGCATTATGGGCAGTg     >  1:8258348/1‑94 (MQ=255)
                                                                 ggCTACAGCCTGAAGGATT‑GTATTGCGTTGGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGC‑TGCATTATGGGCAGTg     <  2:8197733/94‑1 (MQ=255)
                                                                  gCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGC‑TGCATTATGGGCAGTgc    <  2:2298838/94‑2 (MQ=255)
                                                                   cTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGC‑TGCATTATGGGCAGTgcg   >  2:6649350/1‑92 (MQ=255)
                                                                   cTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGC‑TGCATTATGGGCAGTgcg   >  1:5564699/1‑92 (MQ=255)
                                                                   cTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGC‑TGCATTATGGGCAGTgcg   <  1:3271413/94‑3 (MQ=255)
                                                                    tACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAA‑GCATGCATTATGGGTAGTgcgc  >  2:10097940/1‑91 (MQ=255)
                                                                         |                                                                                          
GTCTGGAAGTGATGGCGGGCGGCGTTTC‑AAAGGCCATGCGCTGGAAG‑GCTGGCG‑‑‑‑AACT‑GGCTACAG‑CTGAAGGATT‑G‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGAAA‑GC‑TGCATTATGGGCAGTGGCC  >  NC_000913/4008345‑4008493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 208 total aligned reads displayed.
TTCAAAGGCCATGCGCTGGAAG‑GCTGGCG‑‑‑‑AACT‑G‑GCTACAG‑CTGAAGGATT‑G‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑AAAGCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGAC  >  NC_000913/4008370‑4008510
                                                            |                                                                                                
tcaAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAA                                                               >  SRR4302545.4767244/4‑94 (MQ=16)
 caAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT                                                              <  SRR4302545.8480524/92‑1 (MQ=18)
 caAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT                                                              <  SRR4302545.8435863/92‑1 (MQ=18)
 caAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT                                                              <  SRR4302545.2575333/92‑1 (MQ=18)
 caAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT                                                              >  SRR4302545.2354825/3‑94 (MQ=18)
 caAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT                                                              <  SRR4302545.2348517/92‑1 (MQ=18)
 caAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT                                                              <  SRR4302545.8579375/92‑1 (MQ=18)
 caAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT                                                              <  SRR4302545.8387026/92‑1 (MQ=18)
  AAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATG                                                             >  SRR4302545.312860/1‑94 (MQ=19)
  AAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATG                                                             >  SRR4302545.3592584/1‑94 (MQ=19)
  AAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATG                                                             >  SRR4302545.4910112/1‑94 (MQ=19)
  AAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATG                                                             >  SRR4302545.874445/1‑94 (MQ=19)
  AAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATG                                                             >  SRR4302545.5616317/1‑94 (MQ=19)
   AAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGC                                                            <  SRR4302545.1035173/94‑1 (MQ=21)
     AGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTG                                                          <  SRR4302545.10720851/94‑1 (MQ=23)
     AGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTG                                                          <  SRR4302545.5627053/94‑1 (MQ=23)
     AGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTG                                                          >  SRR4302545.287453/1‑94 (MQ=23)
      tgccatgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGT                                                         >  SRR4302545.3143726/34‑94 (MQ=36)
      GGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGT                                                         <  SRR4302545.6870088/94‑1 (MQ=25)
       GCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTC                                                        <  SRR4302545.5377252/94‑1 (MQ=26)
       GCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTC                                                        <  SRR4302545.7325608/94‑1 (MQ=26)
        CCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCG                                                       <  SRR4302545.10756070/94‑1 (MQ=28)
        CCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCG                                                       <  SRR4302545.1999026/94‑1 (MQ=28)
        CCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCG                                                       <  SRR4302545.5176382/94‑1 (MQ=28)
        CCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCG                                                       >  SRR4302545.1430924/1‑94 (MQ=28)
          atgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                     >  SRR4302545.9191077/30‑94 (MQ=45)
          atgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                     >  SRR4302545.7973788/30‑94 (MQ=45)
          atgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                     <  SRR4302545.3770210/65‑1 (MQ=45)
          atgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                     <  SRR4302545.10200980/65‑1 (MQ=45)
          atgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                     >  SRR4302545.10257309/30‑94 (MQ=45)
          atgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                     <  SRR4302545.1609605/65‑1 (MQ=45)
          atgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                     >  SRR4302545.10257310/30‑94 (MQ=45)
          atgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                     <  SRR4302545.2072725/65‑1 (MQ=45)
          atgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                     <  SRR4302545.1925737/65‑1 (MQ=45)
           tgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG                                                    <  SRR4302545.4651061/66‑1 (MQ=47)
           tgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG                                                    <  SRR4302545.8547752/66‑1 (MQ=47)
           tgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG                                                    <  SRR4302545.8547751/66‑1 (MQ=47)
           tgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACAGGATGAACGACGCCGAAATGCTGTCGATG                                                    <  SRR4302545.6922890/66‑1 (MQ=33)
           tgcgctggaagcggtggcgaagaaacggG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG                                                    <  SRR4302545.3225758/66‑1 (MQ=47)
            gcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG                                                   >  SRR4302545.250520/28‑94 (MQ=49)
            gcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG                                                   >  SRR4302545.10067493/28‑94 (MQ=49)
            gcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG                                                   <  SRR4302545.173343/67‑1 (MQ=49)
            gcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG                                                   <  SRR4302545.8176748/67‑1 (MQ=49)
             cgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGG                                                  >  SRR4302545.9244872/27‑94 (MQ=48)
             cgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC                                                  <  SRR4302545.1747799/68‑1 (MQ=51)
             cgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC                                                  <  SRR4302545.9103505/68‑1 (MQ=51)
              gctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGTTGTCGATGGC‑‑G                                               >  SRR4302545.2127916/26‑94 (MQ=39)
              gctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G                                               <  SRR4302545.10832781/69‑1 (MQ=53)
              gctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G                                               >  SRR4302545.8562300/26‑94 (MQ=53)
              gctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G                                               <  SRR4302545.6111778/69‑1 (MQ=53)
              gctggaagcgggggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGGTGGC‑‑G                                               >  SRR4302545.8562301/26‑94 (MQ=39)
               ctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GG                                              >  SRR4302545.8077099/25‑94 (MQ=55)
                tggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG                                             >  SRR4302545.7317854/24‑94 (MQ=57)
                tggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG                                             >  SRR4302545.8654571/24‑94 (MQ=57)
                tggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG                                             <  SRR4302545.5270725/71‑1 (MQ=57)
                tggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG                                             >  SRR4302545.9437685/24‑94 (MQ=57)
                tggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG                                             <  SRR4302545.10079788/71‑1 (MQ=57)
                 ggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑G                                          <  SRR4302545.6428066/72‑1 (MQ=56)
                 ggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑G                                          <  SRR4302545.4147849/72‑1 (MQ=56)
                 ggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑G                                          >  SRR4302545.5418160/23‑94 (MQ=56)
                  tgaagcggtgtcgaagaatctggtctacagcCTGAAGGATTGG‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑G                                          <  SRR4302545.1588683/63‑1 (MQ=60)
                  gaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑GA                                         >  SRR4302545.9454680/22‑94 (MQ=56)
                  gaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑GA                                         <  SRR4302545.4514185/73‑1 (MQ=56)
                  gaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑GA                                         >  SRR4302545.1487729/22‑94 (MQ=56)
                   aagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑GAA                                        <  SRR4302545.1008841/74‑1 (MQ=55)
                   aagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑GAA                                        <  SRR4302545.5455299/74‑1 (MQ=55)
                   aagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑GAA                                        <  SRR4302545.1672674/74‑1 (MQ=55)
                   aagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑GAA                                        >  SRR4302545.4116772/21‑94 (MQ=55)
                   aagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑GAA                                        <  SRR4302545.2524331/74‑1 (MQ=55)
                    agcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAA                                        <  SRR4302545.9144131/75‑1 (MQ=54)
                    agcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAA                                        <  SRR4302545.9144130/75‑1 (MQ=54)
                    agcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAA                                        <  SRR4302545.8738634/75‑1 (MQ=54)
                    agcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAA                                        >  SRR4302545.4913966/20‑94 (MQ=54)
                     gcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAAG                                       <  SRR4302545.4463207/76‑1 (MQ=53)
                     gcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAAG                                       <  SRR4302545.4463206/76‑1 (MQ=53)
                     gcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAAG                                       <  SRR4302545.7721502/76‑1 (MQ=53)
                     gcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAAG                                       <  SRR4302545.8626951/76‑1 (MQ=53)
                      cggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaagg                                       >  SRR4302545.1879854/18‑88 (MQ=57)
                      cggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaagg                                       >  SRR4302545.7545326/18‑88 (MQ=57)
                      cggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaagg                                       <  SRR4302545.299968/77‑7 (MQ=57)
                       ggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGCCACCGAAATGCTGTCGATGGC‑‑GGagaaatgc                                      >  SRR4302545.4473925/17‑86 (MQ=29)
                       ggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggc                                      >  SRR4302545.2026337/17‑87 (MQ=57)
                       ggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggc                                      >  SRR4302545.3424649/17‑87 (MQ=57)
                       ggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggc                                      <  SRR4302545.5686161/78‑8 (MQ=57)
                       ggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggc                                      <  SRR4302545.2116743/78‑8 (MQ=57)
                       ggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggc                                      >  SRR4302545.6965074/17‑87 (MQ=57)
                        gtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGtgaaaggct                                     <  SRR4302545.10696059/79‑10 (MQ=55)
                        gtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggct                                     <  SRR4302545.189557/79‑9 (MQ=57)
                        gtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggct                                     <  SRR4302545.2199793/79‑9 (MQ=57)
                        gtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggct                                     >  SRR4302545.5640384/16‑86 (MQ=57)
                        gtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggct                                     <  SRR4302545.2598604/79‑9 (MQ=57)
                        gtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggct                                     <  SRR4302545.10508561/79‑9 (MQ=57)
                         tggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctg                                    <  SRR4302545.491815/80‑10 (MQ=57)
                         tggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctg                                    <  SRR4302545.4970368/80‑10 (MQ=57)
                         tggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctg                                    >  SRR4302545.5167863/15‑85 (MQ=57)
                         gggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctg                                    <  SRR4302545.8742684/80‑10 (MQ=57)
                          ggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctgc                                   >  SRR4302545.571596/14‑84 (MQ=57)
                          ggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctgc                                   <  SRR4302545.4187247/81‑11 (MQ=57)
                          ggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctgc                                   >  SRR4302545.3340190/14‑84 (MQ=57)
                          ggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctgc                                   <  SRR4302545.286979/81‑11 (MQ=57)
                          ggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctgc                                   <  SRR4302545.329098/81‑11 (MQ=57)
                           gcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCA                                  <  SRR4302545.2737884/82‑1 (MQ=50)
                            cgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGT‑‑GGGGAAAGGCTGCAT                                 <  SRR4302545.5034143/83‑1 (MQ=40)
                            cgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCAT                                 <  SRR4302545.778260/83‑1 (MQ=49)
                            cgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCAT                                 >  SRR4302545.4203564/12‑94 (MQ=49)
                            cgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCAT                                 <  SRR4302545.5813065/83‑1 (MQ=49)
                            cgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCAT                                 <  SRR4302545.5053182/83‑1 (MQ=49)
                             gaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCTGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATT                                <  SRR4302545.9350761/84‑1 (MQ=37)
                             gaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATT                                >  SRR4302545.1482517/11‑94 (MQ=49)
                             gaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATT                                >  SRR4302545.4757716/11‑94 (MQ=49)
                             gaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATT                                >  SRR4302545.4277279/11‑94 (MQ=49)
                             gaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATT                                <  SRR4302545.10191557/84‑1 (MQ=49)
                             gaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATT                                >  SRR4302545.10371611/11‑94 (MQ=49)
                             gaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATT                                >  SRR4302545.10842559/11‑94 (MQ=49)
                             gaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATT                                >  SRR4302545.3702744/11‑94 (MQ=49)
                              aagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctggatta                               >  SRR4302545.1375441/10‑80 (MQ=57)
                              aagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTA                               >  SRR4302545.7639809/10‑94 (MQ=48)
                              aagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTA                               >  SRR4302545.8001913/10‑94 (MQ=48)
                               agaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTAT                              <  SRR4302545.4729605/86‑1 (MQ=48)
                               agaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTAT                              <  SRR4302545.305200/86‑1 (MQ=48)
                               agaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTAT                              >  SRR4302545.8437115/9‑94 (MQ=48)
                               agaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTAT                              <  SRR4302545.2413800/86‑1 (MQ=48)
                               agaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTAT                              >  SRR4302545.4264311/9‑94 (MQ=48)
                                gaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATG                             >  SRR4302545.8468224/8‑94 (MQ=49)
                                gaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATG                             >  SRR4302545.1730896/8‑94 (MQ=49)
                                 aaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGG                            <  SRR4302545.8404804/88‑1 (MQ=51)
                                 aaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGG                            >  SRR4302545.8101187/7‑94 (MQ=51)
                                 aaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGG                            >  SRR4302545.7928784/7‑94 (MQ=51)
                                 aaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGG                            <  SRR4302545.7755049/88‑1 (MQ=51)
                                 aaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGG                            <  SRR4302545.10022409/88‑1 (MQ=51)
                                 aaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGG                            >  SRR4302545.3332614/7‑94 (MQ=51)
                                 aaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGG                            >  SRR4302545.3680290/7‑94 (MQ=51)
                                  AACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGG                           <  SRR4302545.2835914/94‑1 (MQ=50)
                                  AACT‑GTGCTACAGCCTGAAGGATT‑GTAGTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGG                           <  SRR4302545.1171544/94‑1 (MQ=40)
                                   ACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGC                          <  SRR4302545.6454083/94‑1 (MQ=53)
                                    ctgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCA                         >  SRR4302545.7987309/4‑94 (MQ=57)
                                    ctgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCA                         <  SRR4302545.3599983/91‑1 (MQ=57)
                                    ctgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGCGACGGGATGAACGAAGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctgcattattggcg                         >  SRR4302545.4655664/4‑74 (MQ=31)
                                     tgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAG                        >  SRR4302545.1231582/3‑94 (MQ=58)
                                     G‑G‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGT                       >  SRR4302545.105536/1‑94 (MQ=60)
                                       G‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCCTTATGGGCAGTG                      >  SRR4302545.8497386/1‑94 (MQ=50)
                                       G‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTG                      <  SRR4302545.1502884/94‑1 (MQ=60)
                                         GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGC                     >  SRR4302545.1400189/1‑94 (MQ=60)
                                          CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGcg                    <  SRR4302545.4869935/94‑3 (MQ=60)
                                          CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGcg                    <  SRR4302545.3351600/94‑3 (MQ=60)
                                          CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGcg                    <  SRR4302545.6397730/94‑3 (MQ=60)
                                          CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGcg                    >  SRR4302545.2681045/1‑92 (MQ=60)
                                          CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGcg                    >  SRR4302545.5711976/1‑92 (MQ=60)
                                          CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGcg                    >  SRR4302545.10718452/1‑92 (MQ=60)
                                           TACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGCTGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAGAGGCTGCATTATGGGCAGTGGGC                   >  SRR4302545.7445607/1‑94 (MQ=40)
                                            ACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGcgca                  >  SRR4302545.9250123/1‑90 (MQ=60)
                                            ACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGcgca                  <  SRR4302545.1585454/94‑5 (MQ=60)
                                            ACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGcgca                  >  SRR4302545.10093443/1‑90 (MQ=60)
                                             CAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGTGGC‑‑AAGGCTGCATTATGGGCAGTGcgtac                 >  SRR4302545.9405280/1‑89 (MQ=51)
                                             CAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGcgcac                 >  SRR4302545.1896862/1‑89 (MQ=60)
                                             CAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGcgcac                 >  SRR4302545.1896861/1‑89 (MQ=60)
                                             CAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGcgcac                 >  SRR4302545.8713038/1‑89 (MQ=60)
                                             CAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGcgcac                 >  SRR4302545.8713037/1‑89 (MQ=60)
                                             CAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGcgcac                 >  SRR4302545.3255126/1‑89 (MQ=60)
                                             CAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGcgcac                 >  SRR4302545.6163185/1‑89 (MQ=60)
                                             CAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGcgcac                 >  SRR4302545.3956803/1‑89 (MQ=60)
                                              agcCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACC                >  SRR4302545.4746905/4‑94 (MQ=60)
                                              agcCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACC                >  SRR4302545.4171883/4‑94 (MQ=60)
                                              agcCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACC                >  SRR4302545.285765/4‑94 (MQ=60)
                                              agcCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACC                <  SRR4302545.2715239/91‑1 (MQ=60)
                                              AC‑CTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCA               <  SRR4302545.2610471/94‑1 (MQ=60)
                                               gcCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCA               >  SRR4302545.768570/3‑94 (MQ=60)
                                               C‑CTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAG              >  SRR4302545.4917408/1‑94 (MQ=60)
                                               C‑CTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAG              <  SRR4302545.4977374/94‑1 (MQ=60)
                                                 CTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGcgcaccagg             >  SRR4302545.8960570/1‑85 (MQ=60)
                                                 CTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGC             >  SRR4302545.3289969/1‑94 (MQ=60)
                                                 CTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGC             <  SRR4302545.553713/94‑1 (MQ=60)
                                                  TGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCG            <  SRR4302545.2426169/94‑1 (MQ=57)
                                                  TGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCG            <  SRR4302545.4043713/94‑1 (MQ=57)
                                                  TGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCG            >  SRR4302545.2397041/1‑94 (MQ=57)
                                                  TGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCG            <  SRR4302545.6617634/94‑1 (MQ=57)
                                                  TGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCG            >  SRR4302545.6798561/1‑94 (MQ=57)
                                                   GAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGTCGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGT           <  SRR4302545.2661731/94‑1 (MQ=46)
                                                   GAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGT           >  SRR4302545.6087898/1‑94 (MQ=57)
                                                    AAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTC          >  SRR4302545.10859226/1‑94 (MQ=57)
                                                    AAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTC          >  SRR4302545.10443577/1‑94 (MQ=57)
                                                    AAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTC          <  SRR4302545.7177697/94‑1 (MQ=57)
                                                     AGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCTTTATGGGCAGTGCGCACCAGCGTCT         <  SRR4302545.10450907/94‑1 (MQ=46)
                                                      GGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTG        >  SRR4302545.2477397/1‑94 (MQ=60)
                                                      GGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTG        <  SRR4302545.5730486/94‑1 (MQ=60)
                                                      GGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTG        >  SRR4302545.2704431/1‑94 (MQ=60)
                                                      GGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTG        >  SRR4302545.1541333/1‑94 (MQ=60)
                                                      GGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTG        >  SRR4302545.3464165/1‑94 (MQ=60)
                                                       GATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGA       >  SRR4302545.2626314/1‑94 (MQ=60)
                                                       GATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGA       <  SRR4302545.3728847/94‑1 (MQ=60)
                                                        ATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCCGCGTCTGAA      >  SRR4302545.5170333/1‑94 (MQ=53)
                                                        ATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAA      <  SRR4302545.403852/94‑1 (MQ=60)
                                                         TT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAA     <  SRR4302545.2336932/94‑1 (MQ=60)
                                                         TT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAA     >  SRR4302545.6020995/1‑94 (MQ=60)
                                                         TT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAA     >  SRR4302545.6015247/1‑94 (MQ=60)
                                                            T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAC  >  SRR4302545.2864931/1‑94 (MQ=60)
                                                            T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAC  <  SRR4302545.4523898/94‑1 (MQ=60)
                                                            T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAC  >  SRR4302545.5551456/1‑94 (MQ=60)
                                                            T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAC  <  SRR4302545.9434067/94‑1 (MQ=60)
                                                            T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAC  >  SRR4302545.10084193/1‑94 (MQ=60)
                                                            |                                                                                                
TTCAAAGGCCATGCGCTGGAAG‑GCTGGCG‑‑‑‑AACT‑G‑GCTACAG‑CTGAAGGATT‑G‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑AAAGCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGAC  >  NC_000913/4008370‑4008510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑