Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,008,525 +G intergenic (+92/‑141) b4555 → / → yigM hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,008,5241.G99.7% 2020.6 / ‑2.3 573intergenic (+91/‑142)b4555/yigMhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base G (287/284);  total (287/286)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 625 total aligned reads displayed.

ACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGAAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTG  >  NC_000913/4008433‑4008612
                                                                                              |                                                                                        
aCGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCt                                                                                           >  1:555199/1‑94 (MQ=255)
aCGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCt                                                                                           <  2:9533196/94‑1 (MQ=255)
 cGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑g                                                                                         <  1:6269836/94‑1 (MQ=255)
  gggATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCGGTGCGCGCCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gg                                                                                        <  2:1797330/94‑2 (MQ=255)
  gggATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gg                                                                                        >  1:4947671/1‑93 (MQ=255)
  gggATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gg                                                                                        <  2:1879152/94‑2 (MQ=255)
  gggATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gg                                                                                        >  1:3290467/1‑93 (MQ=255)
   ggATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGCCCTTCATCCAGCGCT‑gga                                                                                       >  2:1330960/1‑92 (MQ=255)
   ggATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gga                                                                                       <  1:828341/94‑3 (MQ=255)
    gATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                                                                                      <  1:4104435/94‑4 (MQ=255)
    gATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                                                                                      <  1:2120915/94‑4 (MQ=255)
       gAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTg                                                                                    >  1:2990349/1‑94 (MQ=255)
        aaCGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                                                                                   >  1:8548317/1‑94 (MQ=255)
         acgacgCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAt                                                                                  <  1:3114285/94‑1 (MQ=255)
          cgacgCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                                                                                 >  2:8222654/1‑94 (MQ=255)
            acgCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGCAAGACCTTCATCCCGAGCTGGAAGTGATTgg                                                                               >  2:8771469/1‑94 (MQ=255)
            acgCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAATGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTgg                                                                               >  1:2874349/1‑94 (MQ=255)
              gCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTa                                                                             <  2:8475781/94‑1 (MQ=255)
              gCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTa                                                                             >  1:1002325/1‑94 (MQ=255)
               ccGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAc                                                                            <  1:2499528/94‑1 (MQ=255)
                 gAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTa                                                                          >  2:2746220/1‑94 (MQ=255)
                 gAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTa                                                                          >  1:9013836/1‑94 (MQ=255)
                 gAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTa                                                                          <  2:3659220/94‑1 (MQ=255)
                 gAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTa                                                                          >  2:5040869/1‑94 (MQ=255)
                   aaTGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt                                                                        <  1:7945278/94‑1 (MQ=255)
                   aaTGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt                                                                        <  2:2149986/94‑1 (MQ=255)
                    aTGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATg                                                                       >  1:117984/1‑94 (MQ=255)
                    aTGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATg                                                                       <  2:7982260/94‑1 (MQ=255)
                    aTGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATg                                                                       >  1:613124/1‑94 (MQ=255)
                       cTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg                                                                    <  1:5038496/94‑1 (MQ=255)
                       cTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg                                                                    >  1:1997155/1‑94 (MQ=255)
                        tGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcga                                                                   >  2:1036164/1‑94 (MQ=255)
                         gTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgac                                                                  <  1:6993022/94‑1 (MQ=255)
                         gTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgac                                                                  <  2:4735414/94‑1 (MQ=255)
                          tCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacg                                                                 >  2:6744316/1‑94 (MQ=255)
                          tCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacg                                                                 <  2:9180141/94‑1 (MQ=255)
                          gcGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacg                                                                 <  2:4097063/93‑1 (MQ=255)
                           cGATGGCGGGGAAATGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga                                                                <  2:2539768/94‑1 (MQ=255)
                           cGATGGCGGGGAAAGGCTGCATTCTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga                                                                >  1:6720739/1‑94 (MQ=255)
                            gATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCgacgac                                                               >  1:10174608/1‑94 (MQ=255)
                             aTGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGacgacg                                                              >  2:611664/1‑94 (MQ=255)
                             aTGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGacgacg                                                              >  2:10075810/1‑94 (MQ=255)
                             aTGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGacgacg                                                              <  1:1334173/94‑1 (MQ=255)
                               ggcggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAcgcg                                                            <  2:5977123/90‑1 (MQ=255)
                               ggcggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAcgcg                                                            <  2:9841800/90‑1 (MQ=255)
                                  ggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTg                                                         >  2:75557/2‑94 (MQ=255)
                                  ggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTACATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTg                                                         >  1:1710749/2‑94 (MQ=255)
                                  ggggAAAGGCTGCATTATGGGCAGCGCGCACCATCGTCTGAAAGACCTTCATCCCGACCTGGAATGGATTGGTACTAATGCCGACGACGCGTTg                                                         >  2:4249346/2‑94 (MQ=255)
                                   gggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGc                                                        >  1:7208197/1‑94 (MQ=255)
                                      aaaggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGc                                                     >  2:6472131/5‑94 (MQ=255)
                                         ggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAtt                                                  <  2:7710500/93‑1 (MQ=255)
                                         ggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCACAtt                                                  >  2:5214793/2‑94 (MQ=255)
                                            tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATc                                               <  1:8395781/94‑1 (MQ=255)
                                             gCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt                                              <  2:7291882/94‑1 (MQ=255)
                                              gaTTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTg                                             <  2:3495309/93‑1 (MQ=255)
                                              cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTg                                             >  2:4396132/1‑94 (MQ=255)
                                              cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTg                                             <  1:4599552/94‑1 (MQ=255)
                                                ttATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCg                                           >  1:963422/1‑94 (MQ=255)
                                                  aTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTa                                         >  1:5612261/1‑94 (MQ=255)
                                                   tGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa                                        <  2:2406632/94‑1 (MQ=255)
                                                    gggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa                                       >  2:8623291/1‑94 (MQ=255)
                                                      gCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAct                                     <  1:9058941/94‑1 (MQ=255)
                                                          tgcgcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAt                                 <  1:9990461/90‑1 (MQ=255)
                                                           gcgcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAtt                                >  2:6989230/4‑94 (MQ=255)
                                                             gcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTa                              <  2:4948132/93‑1 (MQ=255)
                                                             gcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTa                              >  1:10351187/2‑94 (MQ=255)
                                                             gcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTa                              <  2:170961/93‑1 (MQ=255)
                                                              caccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTAt                             <  1:4315588/94‑1 (MQ=255)
                                                              caccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTAt                             <  2:161150/94‑1 (MQ=255)
                                                              caccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTAt                             <  1:654847/94‑1 (MQ=255)
                                                               accaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATc                            >  2:33263/1‑94 (MQ=255)
                                                               accaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATc                            <  2:109521/94‑1 (MQ=255)
                                                               accaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATc                            >  2:458220/1‑94 (MQ=255)
                                                                ccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCg                           <  1:3480540/94‑1 (MQ=255)
                                                                ccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCg                           >  2:10451916/1‑94 (MQ=255)
                                                                ccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCg                           <  2:3878589/94‑1 (MQ=255)
                                                                ccaGCGTCTGAAAGACCGTCATCCCGAGCTGGAAGTGATTCGTACTAATTCCGACGCCGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCg                           <  2:5612261/94‑1 (MQ=255)
                                                                 caGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGt                          >  2:2070032/1‑94 (MQ=255)
                                                                 caGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGt                          >  1:1448081/1‑94 (MQ=255)
                                                                 caGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGt                          <  2:5542562/94‑1 (MQ=255)
                                                                 caGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGt                          <  1:7409542/94‑1 (MQ=255)
                                                                  cgCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTa                         <  1:5460470/93‑1 (MQ=255)
                                                                  aGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTCAGTAAACTCTATTTATCGTa                         >  1:5220712/1‑94 (MQ=255)
                                                                    cGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAAt                       >  1:3794933/1‑94 (MQ=255)
                                                                         gAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTc                  <  2:8877248/94‑1 (MQ=255)
                                                                         gAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAACTGCGTAAACTCTATTTATCGTAATCGttt                  >  2:940396/1‑93 (MQ=255)
                                                                          aaaGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCAGCATTATCTGCGTAAACTCTATATATCGTAATCGTTCt                 >  1:9240024/1‑94 (MQ=255)
                                                                           aaGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCtt                >  1:8126377/1‑94 (MQ=255)
                                                                           aaGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCtt                >  1:8825694/1‑94 (MQ=255)
                                                                             gACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTa              <  1:3948378/94‑1 (MQ=255)
                                                                              aCCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAt             >  2:2937674/1‑94 (MQ=255)
                                                                                cTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAttt           >  2:1187038/1‑94 (MQ=255)
                                                                                 ttCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTg          >  1:5959731/1‑94 (MQ=255)
                                                                                   cATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGt        >  1:7539337/1‑94 (MQ=255)
                                                                                    aTCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTc       >  1:10544469/1‑94 (MQ=255)
                                                                                      cccGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAg     <  1:3642353/94‑1 (MQ=255)
                                                                                        cGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGtt   >  2:2860920/1‑94 (MQ=255)
                                                                                        cGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGtt   <  1:468644/94‑1 (MQ=255)
                                                                                        cGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGtt   >  1:5795826/1‑94 (MQ=255)
                                                                                         gAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTg  <  1:2255919/94‑1 (MQ=255)
                                                                                              |                                                                                        
ACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGAAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTG  >  NC_000913/4008433‑4008612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 292 total aligned reads displayed.
GCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCCG‑‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACGCCACTG  >  NC_000913/4013083‑4013262
                                                                                             |                                                                                        
GCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGGTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCG                                                                                          >  SRR4302545.10666131/1‑94 (MQ=60)
 CTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGG                                                                                         >  SRR4302545.9958303/1‑94 (MQ=60)
 CTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGG                                                                                         <  SRR4302545.2993006/94‑1 (MQ=60)
 CTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGG                                                                                         >  SRR4302545.9465308/1‑94 (MQ=60)
 CTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGG                                                                                         <  SRR4302545.6172541/94‑1 (MQ=60)
  TTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAAT‑CG‑‑GC                                                                                     >  SRR4302545.6438000/1‑94 (MQ=60)
   TTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAAT‑CG‑‑GCG                                                                                    >  SRR4302545.7162881/1‑94 (MQ=60)
   TTCGCATTAGCAGCACGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAAT‑CG‑‑GCG                                                                                    <  SRR4302545.4579648/94‑1 (MQ=60)
        ATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTA                                                                                 <  SRR4302545.1184520/94‑1 (MQ=60)
        ATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTA                                                                                 >  SRR4302545.6320623/1‑94 (MQ=60)
        ATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTA                                                                                 >  SRR4302545.1011817/1‑94 (MQ=60)
        ATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTA                                                                                 >  SRR4302545.5599596/1‑94 (MQ=60)
         TTAGCAGCCCGCGGCGCCTGGCGCATGGTTTCAACGCGCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAA                                                                                <  SRR4302545.9124237/94‑1 (MQ=60)
          TAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAG                                                                               <  SRR4302545.2621234/94‑1 (MQ=60)
            GCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGAT                                                                             >  SRR4302545.6007320/1‑94 (MQ=60)
            GCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGAT                                                                             <  SRR4302545.2757585/94‑1 (MQ=60)
             CAGCCCGCAGCGCCTGGCGCATGTTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAAAATCGGC‑GCGTTAAGATC                                                                            <  SRR4302545.9484769/94‑1 (MQ=60)
             CAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATC                                                                            <  SRR4302545.5827591/94‑1 (MQ=60)
             CAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATC                                                                            >  SRR4302545.9002265/1‑94 (MQ=60)
              CGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCG                                                                           <  SRR4302545.2681925/94‑1 (MQ=60)
              AGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCG                                                                           >  SRR4302545.4637364/1‑94 (MQ=60)
              AGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCG                                                                           <  SRR4302545.3852151/94‑1 (MQ=60)
              AGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCG                                                                           <  SRR4302545.3466653/94‑1 (MQ=60)
              AGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCG                                                                           >  SRR4302545.9763238/1‑94 (MQ=60)
              AGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAAAATCGGC‑GCGTTACGATCG                                                                           >  SRR4302545.3545734/1‑94 (MQ=60)
               GCCCGCAGCGCCTGGCGCATGGTTTCAACTCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGT‑GCGTTAAGATCGG                                                                          <  SRR4302545.7829835/94‑1 (MQ=60)
               GCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGT‑GCGTTAAGATCGG                                                                          <  SRR4302545.7829834/94‑1 (MQ=60)
               GCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGG                                                                          <  SRR4302545.9327151/94‑1 (MQ=60)
                CCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGT                                                                         >  SRR4302545.4297674/1‑94 (MQ=60)
                 CCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTT                                                                        <  SRR4302545.10932090/94‑1 (MQ=60)
                 CCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTT                                                                        >  SRR4302545.8169361/1‑94 (MQ=60)
                 CCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTT                                                                        <  SRR4302545.3472759/94‑1 (MQ=60)
                 CCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTT                                                                        <  SRR4302545.96817/94‑1 (MQ=60)
                  CGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTG                                                                       >  SRR4302545.9425869/1‑94 (MQ=60)
                  CGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTG                                                                       <  SRR4302545.1748962/94‑1 (MQ=60)
                  CGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTG                                                                       <  SRR4302545.7275008/94‑1 (MQ=60)
                  CGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTG                                                                       <  SRR4302545.849139/94‑1 (MQ=60)
                  CGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGACGAAAATCGGC‑GCGTTAAGATCGGTTG                                                                       >  SRR4302545.5954281/1‑94 (MQ=60)
                   GCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGC                                                                      >  SRR4302545.4567273/1‑94 (MQ=60)
                   GCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGC                                                                      >  SRR4302545.5136113/1‑94 (MQ=60)
                    CAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCG                                                                     >  SRR4302545.6746682/1‑94 (MQ=60)
                    CAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCG                                                                     >  SRR4302545.551693/1‑94 (MQ=60)
                     AGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATCTAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGA                                                                    >  SRR4302545.9470994/1‑94 (MQ=60)
                      GCGCCTGGCGCATTGTTTCAACGCTTTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGAT                                                                   <  SRR4302545.3257410/94‑1 (MQ=60)
                      GCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGAT                                                                   <  SRR4302545.2362786/94‑1 (MQ=60)
                       CGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATA                                                                  >  SRR4302545.1094829/1‑94 (MQ=60)
                       CGCCTGGCGCATGGTGTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATA                                                                  <  SRR4302545.257576/94‑1 (MQ=60)
                        GCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATAT                                                                 <  SRR4302545.4620775/94‑1 (MQ=60)
                        GCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATAT                                                                 <  SRR4302545.3562721/94‑1 (MQ=60)
                        GCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATAT                                                                 <  SRR4302545.5303711/94‑1 (MQ=60)
                        GCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATAT                                                                 <  SRR4302545.893744/94‑1 (MQ=60)
                        GCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATAT                                                                 >  SRR4302545.2988886/1‑94 (MQ=60)
                        GCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATAT                                                                 >  SRR4302545.9860591/1‑94 (MQ=60)
                         CCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATC                                                                <  SRR4302545.581097/94‑1 (MQ=60)
                         CCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATC                                                                <  SRR4302545.9186238/94‑1 (MQ=60)
                           TGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAA                                                              >  SRR4302545.3569921/1‑94 (MQ=60)
                            GGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAAC                                                             >  SRR4302545.3051889/1‑94 (MQ=60)
                            GGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAAC                                                             >  SRR4302545.7786629/1‑94 (MQ=60)
                             GCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACA                                                            >  SRR4302545.2486444/1‑94 (MQ=60)
                             GCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACA                                                            >  SRR4302545.3662424/1‑94 (MQ=60)
                             GCGCATAGTTTCACCGCTCTCCTACGGAATGCTGTTATCCTGACCACCATATAAGCGGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACA                                                            <  SRR4302545.6079566/94‑1 (MQ=60)
                              CGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAG                                                           >  SRR4302545.1560106/1‑94 (MQ=60)
                              CGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAG                                                           >  SRR4302545.4283712/1‑94 (MQ=60)
                              CGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAG                                                           >  SRR4302545.8661096/1‑94 (MQ=60)
                              CGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTCCGATATCAACAG                                                           >  SRR4302545.1829471/1‑94 (MQ=60)
                               GCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGG                                                          <  SRR4302545.5039832/94‑1 (MQ=60)
                               GCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGG                                                          >  SRR4302545.1295725/1‑94 (MQ=60)
                               GCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGG                                                          >  SRR4302545.3444008/1‑94 (MQ=60)
                                CATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGT                                                         <  SRR4302545.4702728/94‑1 (MQ=60)
                                 ATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTT                                                        <  SRR4302545.6899396/94‑1 (MQ=60)
                                 ATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTT                                                        >  SRR4302545.10230354/1‑94 (MQ=60)
                                 ATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTT                                                        <  SRR4302545.8757237/94‑1 (MQ=60)
                                 ATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTT                                                        <  SRR4302545.2053883/94‑1 (MQ=60)
                                  TGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTG                                                       <  SRR4302545.7292266/94‑1 (MQ=60)
                                  TGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTG                                                       <  SRR4302545.10452250/94‑1 (MQ=60)
                                  TGGTTTCAACGCTCTCCTGCAGAATGCTGTTATCCTGACCACAATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTG                                                       <  SRR4302545.9947925/94‑1 (MQ=60)
                                  TGGGTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTG                                                       >  SRR4302545.8064647/1‑94 (MQ=60)
                                   GGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGT                                                      <  SRR4302545.8297399/94‑1 (MQ=60)
                                     TTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTT                                                    <  SRR4302545.7631033/94‑1 (MQ=60)
                                       TCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCG                                                  <  SRR4302545.2732726/94‑1 (MQ=60)
                                        CAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGG                                                 <  SRR4302545.2239628/94‑1 (MQ=60)
                                        CAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGG                                                 >  SRR4302545.89044/1‑94 (MQ=60)
                                         acccCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGT                                                <  SRR4302545.1996280/90‑1 (MQ=60)
                                         AGCGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGT                                                <  SRR4302545.5203171/94‑1 (MQ=60)
                                         AACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGT                                                <  SRR4302545.5696472/94‑1 (MQ=60)
                                         AACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGT                                                >  SRR4302545.1386100/1‑94 (MQ=60)
                                         AACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGT                                                >  SRR4302545.9440077/1‑94 (MQ=60)
                                         AACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGT                                                >  SRR4302545.8917431/1‑94 (MQ=60)
                                         AACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGT                                                >  SRR4302545.6441037/1‑94 (MQ=60)
                                         AACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGT                                                >  SRR4302545.6570498/1‑94 (MQ=60)
                                          ACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTG                                               >  SRR4302545.2267699/1‑94 (MQ=60)
                                           CGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGA                                              >  SRR4302545.6390190/1‑94 (MQ=60)
                                           CGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGA                                              <  SRR4302545.1600501/94‑1 (MQ=60)
                                           CGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGA                                              >  SRR4302545.513824/1‑94 (MQ=60)
                                            GCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACATGTTGTTTCGGTGAA                                             >  SRR4302545.10747648/1‑94 (MQ=60)
                                            GCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAA                                             >  SRR4302545.6206600/1‑94 (MQ=60)
                                            GCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAA                                             >  SRR4302545.6491669/1‑94 (MQ=60)
                                            GCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAA                                             <  SRR4302545.3021444/94‑1 (MQ=60)
                                              TCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATT                                           <  SRR4302545.5049337/94‑1 (MQ=60)
                                              TCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATT                                           <  SRR4302545.8968014/94‑1 (MQ=60)
                                              TCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATT                                           >  SRR4302545.10734438/1‑94 (MQ=60)
                                               CTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTC                                          >  SRR4302545.4032434/1‑94 (MQ=60)
                                               CTCCTGCGGAATGCTGTGATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTC                                          <  SRR4302545.6325543/94‑1 (MQ=60)
                                                TCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTATGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCA                                         >  SRR4302545.10564143/1‑94 (MQ=60)
                                                TCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCA                                         <  SRR4302545.7623569/94‑1 (MQ=60)
                                                TCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCA                                         >  SRR4302545.7048030/1‑94 (MQ=60)
                                                CCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCA                                         <  SRR4302545.3982215/94‑1 (MQ=60)
                                                 ctggcgaAATGCTTTTATCCGGACCCCCATAAAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAG                                        <  SRR4302545.2784273/87‑1 (MQ=60)
                                                 CCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAG                                        >  SRR4302545.384730/1‑94 (MQ=60)
                                                 CCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAG                                        <  SRR4302545.5738470/94‑1 (MQ=60)
                                                  CTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGC                                       <  SRR4302545.8807930/94‑1 (MQ=60)
                                                  CTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGC                                       <  SRR4302545.7160124/94‑1 (MQ=60)
                                                  CTGCGGAATGCTGTTATCCTGACCACAATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGC                                       <  SRR4302545.4020799/94‑1 (MQ=60)
                                                   TGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCG                                      <  SRR4302545.4073805/94‑1 (MQ=60)
                                                   TGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCG                                      <  SRR4302545.9816085/94‑1 (MQ=60)
                                                   TGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAA‑CCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCG                                      <  SRR4302545.7718039/94‑1 (MQ=60)
                                                     CGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTagatcagagatcggaag                                    >  SRR4302545.7876991/1‑77 (MQ=60)
                                                      GGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACT                                   <  SRR4302545.8555654/94‑1 (MQ=60)
                                                       GAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTT                                  <  SRR4302545.6715113/94‑1 (MQ=60)
                                                       GAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTT                                  >  SRR4302545.10731675/1‑94 (MQ=60)
                                                       GAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTT                                  <  SRR4302545.6715119/94‑1 (MQ=60)
                                                       GAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTT                                  >  SRR4302545.2544166/1‑94 (MQ=60)
                                                       GAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTT                                  >  SRR4302545.8991885/1‑94 (MQ=60)
                                                       GAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTT                                  >  SRR4302545.1165179/1‑94 (MQ=60)
                                                        AATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTG                                 >  SRR4302545.3349971/1‑94 (MQ=60)
                                                        AATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTG                                 >  SRR4302545.3069850/1‑94 (MQ=60)
                                                        AATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTA                                 <  SRR4302545.7027469/94‑1 (MQ=60)
                                                        AATGCTGTTATCCTGACAACCATAAAAACAGAAAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTG                                 <  SRR4302545.9623715/94‑1 (MQ=60)
                                                         ATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGT                                >  SRR4302545.6917257/1‑94 (MQ=60)
                                                         ATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGT                                >  SRR4302545.684217/1‑94 (MQ=60)
                                                          TGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTC                               >  SRR4302545.8969402/1‑94 (MQ=60)
                                                          TGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTC                               >  SRR4302545.2472283/1‑94 (MQ=60)
                                                          TGCTGTTATCCTGACCACCATATAAGACGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTC                               >  SRR4302545.4103993/1‑94 (MQ=60)
                                                           GCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCG                              >  SRR4302545.8894223/1‑94 (MQ=60)
                                                           GCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCG                              >  SRR4302545.3267995/1‑94 (MQ=60)
                                                           GCTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCG                              <  SRR4302545.6252298/94‑1 (MQ=60)
                                                            CTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGC                             <  SRR4302545.5186504/94‑1 (MQ=60)
                                                            CTGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGC                             >  SRR4302545.257916/1‑94 (MQ=60)
                                                             TGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCC                            >  SRR4302545.6576527/1‑94 (MQ=60)
                                                             TGTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCC                            >  SRR4302545.7475632/1‑94 (MQ=60)
                                                              GTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCC                           >  SRR4302545.10183885/1‑94 (MQ=60)
                                                              GTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCC                           >  SRR4302545.10817096/1‑94 (MQ=60)
                                                              GTTATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCC                           <  SRR4302545.9000084/94‑1 (MQ=60)
                                                                TATCCTGACCACCATATAAGCCTAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGT                         <  SRR4302545.8017854/94‑1 (MQ=60)
                                                                TATCCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGCTGTTTCGGTGAATTCAGCGACTTGTCGCCCGC                         >  SRR4302545.5229126/1‑94 (MQ=60)
                                                                   CCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAG                      <  SRR4302545.377783/94‑1 (MQ=60)
                                                                   CCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAG                      <  SRR4302545.10262645/94‑1 (MQ=60)
                                                                   CCTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAG                      >  SRR4302545.4541585/1‑94 (MQ=60)
                                                                    CTGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGACGCCCGTCAGT                     >  SRR4302545.1392724/1‑94 (MQ=60)
                                                                    CAGACCACGATATAAGCCGAGATTCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGT                     <  SRR4302545.4381149/94‑1 (MQ=60)
                                                                     TGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGGCTTGTCACCCGTCAGTT                    >  SRR4302545.3717967/1‑94 (MQ=60)
                                                                     TGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTT                    >  SRR4302545.7376829/1‑94 (MQ=60)
                                                                     TGACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTT                    <  SRR4302545.3361564/94‑1 (MQ=60)
                                                                      GACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTT                   >  SRR4302545.4223942/1‑94 (MQ=60)
                                                                      GACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTT                   <  SRR4302545.4306408/94‑1 (MQ=60)
                                                                      GACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTT                   >  SRR4302545.8444222/1‑94 (MQ=60)
                                                                      GACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTT                   <  SRR4302545.9111586/94‑1 (MQ=60)
                                                                      GACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTT                   >  SRR4302545.6385802/1‑94 (MQ=60)
                                                                      GACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTT                   <  SRR4302545.9156750/94‑1 (MQ=60)
                                                                      GACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTT                   <  SRR4302545.6934727/94‑1 (MQ=60)
                                                                      GACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTT                   <  SRR4302545.9283484/94‑1 (MQ=60)
                                                                       ACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTG                  >  SRR4302545.859476/1‑94 (MQ=60)
                                                                       ACCACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTG                  <  SRR4302545.4837522/94‑1 (MQ=60)
                                                                         CACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCC                <  SRR4302545.10548004/94‑1 (MQ=60)
                                                                         CACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCC                >  SRR4302545.8280849/1‑94 (MQ=60)
                                                                         CACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCC                >  SRR4302545.1748389/1‑94 (MQ=60)
                                                                         CACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCC                >  SRR4302545.5832370/1‑94 (MQ=60)
                                                                         CACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCC                <  SRR4302545.10656332/94‑1 (MQ=60)
                                                                          CCCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCG               <  SRR4302545.3158463/94‑1 (MQ=60)
                                                                          ACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCG               <  SRR4302545.3158461/94‑1 (MQ=60)
                                                                          ACCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCG               >  SRR4302545.10685311/1‑94 (MQ=60)
                                                                           CCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTGAGTTTGCCGT              >  SRR4302545.2514244/1‑94 (MQ=60)
                                                                           CCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGT              >  SRR4302545.2653919/1‑94 (MQ=60)
                                                                           CCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGT              <  SRR4302545.5903723/94‑1 (MQ=60)
                                                                           CCATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGT              >  SRR4302545.6769081/1‑94 (MQ=60)
                                                                            CATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTA             >  SRR4302545.10036992/1‑94 (MQ=60)
                                                                            CATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTA             >  SRR4302545.10036991/1‑94 (MQ=60)
                                                                            CATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTA             >  SRR4302545.6498710/1‑94 (MQ=60)
                                                                            CATATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTA             <  SRR4302545.7819495/94‑1 (MQ=60)
                                                                              TATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACC           >  SRR4302545.443120/1‑94 (MQ=60)
                                                                              TATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCCTCAGTTTGCCGTACC           >  SRR4302545.6120288/1‑94 (MQ=60)
                                                                              TATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGACCGTCAGTTTGCCGTACC           >  SRR4302545.8563843/1‑94 (MQ=60)
                                                                               ATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCA          >  SRR4302545.7246518/1‑94 (MQ=60)
                                                                               ATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCA          >  SRR4302545.7088991/1‑94 (MQ=60)
                                                                               ATAAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCA          <  SRR4302545.5452350/94‑1 (MQ=60)
                                                                                 AAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACG        >  SRR4302545.10406697/1‑94 (MQ=60)
                                                                                 AAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACG        >  SRR4302545.9785615/1‑94 (MQ=60)
                                                                                 AAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACG        >  SRR4302545.4165631/1‑94 (MQ=60)
                                                                                 AAGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACG        >  SRR4302545.4165915/1‑94 (MQ=60)
                                                                                  AGCCGAGAGTCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACGC       >  SRR4302545.7443761/1‑94 (MQ=60)
                                                                                  AGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACGC       >  SRR4302545.961656/1‑94 (MQ=60)
                                                                                  AGCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACGC       >  SRR4302545.10864502/1‑94 (MQ=60)
                                                                                   GCCGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACGCC      <  SRR4302545.6685455/94‑1 (MQ=60)
                                                                                   GCCGAGAATCGGC‑GCGTTAAGATCCGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCACCCATCAGTTTGCCGTACGAGGCC      >  SRR4302545.10772866/1‑94 (MQ=60)
                                                                                    CCGAGAATCGGC‑GCGTTTAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACaagacccccgtcaatttgcccttcctcgccc     >  SRR4302545.9738573/1‑63 (MQ=60)
                                                                                     CGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACGCCAC    <  SRR4302545.10047043/94‑1 (MQ=60)
                                                                                     CGAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACGCCAC    >  SRR4302545.7909647/1‑94 (MQ=60)
                                                                                      GAGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACGCCACT   <  SRR4302545.1795467/94‑1 (MQ=60)
                                                                                       AGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACGCCACTG  >  SRR4302545.8925377/1‑94 (MQ=60)
                                                                                       AGAATCGGC‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACGCCACTG  >  SRR4302545.8306609/1‑94 (MQ=60)
                                                                                             |                                                                                        
GCTTTCGCATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCCG‑‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACGCCACTG  >  NC_000913/4013083‑4013262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑