Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,013,177 +C coding (543/999 nt) ysgA ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,013,1771.C99.5% 1913.9 / ‑4.1 556A181A (GCC→GCG) ysgAhypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base C (284/269);  minor base T (0/3);  total (284/272)
Fisher's exact test for biased strand distribution p-value = 1.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 571 total aligned reads displayed.

ATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCCG‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACGCCACT  >  NC_000913/4013091‑4013261
                                                                                       |                                                                                    
 ttAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTaa                                                                               >  2:336718/1‑94 (MQ=255)
 ttAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTaa                                                                               <  2:4837584/94‑1 (MQ=255)
 ttAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTaa                                                                               >  1:635746/1‑94 (MQ=255)
  tAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAg                                                                              >  2:8522666/1‑94 (MQ=255)
   agcagcCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGa                                                                             >  2:9298814/1‑94 (MQ=255)
    gcagcCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGAt                                                                            >  1:5849786/1‑94 (MQ=255)
    gcagcCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGAt                                                                            <  2:2742935/94‑1 (MQ=255)
     cagcCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATc                                                                           <  1:2687492/94‑1 (MQ=255)
     cagcCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATc                                                                           <  1:9912663/94‑1 (MQ=255)
     cagcCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATc                                                                           >  1:8748324/1‑94 (MQ=255)
       gcCCGCAGCGCCTGGCGCATGGTTTCAACTCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGTGCGTTAAGATCgg                                                                         <  1:7614423/94‑1 (MQ=255)
       gcCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCgg                                                                         <  1:1665587/94‑1 (MQ=255)
       gcCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCgg                                                                         <  2:6548186/94‑1 (MQ=255)
       gcCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCgg                                                                         <  2:7435272/94‑1 (MQ=255)
        cccGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGt                                                                        >  1:4193023/1‑94 (MQ=255)
         ccGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGtt                                                                       <  1:10619466/94‑1 (MQ=255)
          cGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTg                                                                      <  2:1595862/94‑1 (MQ=255)
          cGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTg                                                                      >  2:4996919/1‑94 (MQ=255)
           gCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATCTAAGCCGAGAATCGGCGCGTTAAGATCGGTTGc                                                                     >  2:10241030/1‑94 (MQ=255)
           gCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGc                                                                     <  2:4503585/94‑1 (MQ=255)
            cAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCg                                                                    <  2:5514665/94‑1 (MQ=255)
             aGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGa                                                                   >  2:10037287/1‑94 (MQ=255)
             aGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGa                                                                   >  2:6028858/1‑94 (MQ=255)
             aGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGa                                                                   >  2:1751559/1‑94 (MQ=255)
              gcgcCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGat                                                                  <  2:8861704/94‑1 (MQ=255)
                gcCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGatat                                                                <  1:3477489/94‑1 (MQ=255)
                gcCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGatat                                                                >  2:8123115/1‑94 (MQ=255)
                   tGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCaa                                                             <  2:3953987/94‑1 (MQ=255)
                   tGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCaa                                                             <  2:5429127/94‑1 (MQ=255)
                   tGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCaa                                                             <  2:5655336/94‑1 (MQ=255)
                    ggCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCCCCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAAc                                                            <  1:2452066/94‑1 (MQ=255)
                    ggCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAAc                                                            <  2:2194102/94‑1 (MQ=255)
                     gcgcATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACa                                                           <  2:1908027/94‑1 (MQ=255)
                     gcgcATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACa                                                           <  2:2379473/94‑1 (MQ=255)
                      cgcATGGTTTCAACGCCCTCCTGCGGAATGCTGTTATCCTGACCCCCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAg                                                          <  2:9232565/94‑1 (MQ=255)
                       gcATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAgg                                                         >  2:4752165/1‑94 (MQ=255)
                       gcATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAgg                                                         >  2:10473558/1‑94 (MQ=255)
                       gcATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAgg                                                         >  1:1267394/1‑94 (MQ=255)
                       gcATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAgg                                                         >  1:3361987/1‑94 (MQ=255)
                       gcATGGTTGCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAgg                                                         <  2:6357103/94‑1 (MQ=255)
                        cATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGgt                                                        >  1:8370122/1‑94 (MQ=255)
                        cATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGgt                                                        <  1:4585270/94‑1 (MQ=255)
                         aTGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGgtt                                                       <  1:6715300/94‑1 (MQ=255)
                          tgatttCACCGCTCTCCTGCGGAATGCTGTTATCCTGACCCCCATAAAAGCCGAGCATCGGCGCGTTAAGATCGGTTGCGATATCAACAGgttg                                                      <  1:9382543/91‑1 (MQ=255)
                          tGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGgttg                                                      <  2:7902165/94‑1 (MQ=255)
                          tGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGgttg                                                      >  1:5077033/1‑94 (MQ=255)
                          tGGGTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGgttg                                                      >  1:7842301/1‑94 (MQ=255)
                           ggTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAGGATCGGTTGCGATATCAACAGgttgt                                                     >  1:6224378/1‑94 (MQ=255)
                               tCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCg                                                 <  2:3766331/94‑1 (MQ=255)
                               tCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCg                                                 <  2:4398107/94‑1 (MQ=255)
                                cAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCgg                                                >  2:6186452/1‑94 (MQ=255)
                                cAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCgg                                                <  1:2186216/94‑1 (MQ=255)
                                 aaCGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGt                                               >  1:9171019/1‑94 (MQ=255)
                                 aaCGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGt                                               >  2:2064373/1‑94 (MQ=255)
                                 aaCGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGGTTCggg                                               >  2:7973392/1‑93 (MQ=255)
                                  aCGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTg                                              <  2:7579067/94‑1 (MQ=255)
                                    gCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGTCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGaa                                            >  1:10322722/1‑94 (MQ=255)
                                      tctcCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAAtt                                          <  1:8715135/94‑1 (MQ=255)
                                       ctcCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTc                                         <  2:8244013/94‑1 (MQ=255)
                                        tcCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCa                                        >  1:6859573/1‑94 (MQ=255)
                                          cTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGc                                      >  2:5867162/1‑94 (MQ=255)
                                           tGCGGAATGTTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCg                                     >  1:2553736/1‑94 (MQ=255)
                                           tGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCg                                     <  1:1830058/94‑1 (MQ=255)
                                           tGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAACCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCg                                     <  1:7505663/94‑1 (MQ=255)
                                            gCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGa                                    <  2:1176672/94‑1 (MQ=255)
                                             cGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGgttgtt                                                    <  2:7660202/77‑1 (MQ=255)
                                             cGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGAc                                   <  2:307051/94‑1 (MQ=255)
                                               gAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACtt                                 <  1:6536300/94‑1 (MQ=255)
                                                 aTGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGt                               >  2:2880492/1‑94 (MQ=255)
                                                 aTGCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGt                               >  1:6122712/1‑94 (MQ=255)
                                                   gCTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCg                             >  1:8643538/1‑94 (MQ=255)
                                                    cTGTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGc                            >  2:1459417/1‑94 (MQ=255)
                                                      gTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGccc                          >  1:2958266/1‑94 (MQ=255)
                                                      gTTATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGccc                          >  2:9658415/1‑94 (MQ=255)
                                                      gTTATCCTGACCACCATATAAGACGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGCATTCAGCGACTTGTCGccc                          >  2:9540860/1‑94 (MQ=255)
                                                       ttATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGGTGTTTCGGTGAATGCAGCGGCTTGTCGCCCg                         >  2:908279/1‑94 (MQ=255)
                                                        tATCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGt                        >  2:3651903/1‑94 (MQ=255)
                                                         ttCCTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTc                       <  1:3748699/93‑1 (MQ=255)
                                                           ccTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAg                     <  1:3167725/94‑1 (MQ=255)
                                                           ccTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAg                     >  2:9896745/1‑94 (MQ=255)
                                                           ccTGACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAg                     >  1:4428972/1‑94 (MQ=255)
                                                              gACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGttt                  <  1:8898119/94‑1 (MQ=255)
                                                              gACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGttt                  <  1:3932335/94‑1 (MQ=255)
                                                              gACCACCATATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGttt                  >  1:8206706/1‑94 (MQ=255)
                                                                ccaccaTATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGc                <  1:10430700/94‑1 (MQ=255)
                                                                 caccaTATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGcc               >  1:5680542/1‑94 (MQ=255)
                                                                    caTATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTa            >  1:9746918/1‑94 (MQ=255)
                                                                    caTATAAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTa            >  2:4043317/1‑94 (MQ=255)
                                                                       ataAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCa         >  1:7051607/1‑94 (MQ=255)
                                                                        taAGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCAc        <  2:1267286/94‑1 (MQ=255)
                                                                         aaGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACg       >  1:4064177/1‑94 (MQ=255)
                                                                         aaGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACg       >  1:9505758/1‑94 (MQ=255)
                                                                         aaGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACg       >  1:10107744/1‑94 (MQ=255)
                                                                          aGCCGAGAGTCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACGc      >  1:7241168/1‑94 (MQ=255)
                                                                          aGCCGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACGc      >  2:3256128/1‑94 (MQ=255)
                                                                            ccGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACGCCa    >  2:6680942/1‑94 (MQ=255)
                                                                            ccGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACGCCa    >  1:5007379/1‑94 (MQ=255)
                                                                             cGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACGCCAc   <  2:6842940/94‑1 (MQ=255)
                                                                             cGAGAATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACGCCAc   <  1:5461013/94‑1 (MQ=255)
                                                                              gagaATCGGCGCGTTAAGGTCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACGCCACt  <  2:3500504/94‑1 (MQ=255)
                                                                                       |                                                                                    
ATTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGAATCCG‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACGCCACT  >  NC_000913/4013091‑4013261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 379 total aligned reads displayed.
GTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCA  >  NC_000913/4013624‑4013809
                                                                                              |                                                                                              
GTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC                                                                                                >  SRR4302545.6993180/1‑94 (MQ=60)
 taagccgctatgtaatcccttagtgaagaaaaAGAGGTAAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑G                                                                                              <  SRR4302545.8352815/62‑1 (MQ=60)
 gaagcaggccttttatccttgtttgaaggtagggagggGAAG‑CCCGCCACAATCCCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑T                                                                                              <  SRR4302545.3147733/56‑1 (MQ=60)
  AAGCAGGCATTTTACCCCCTTGTGAAGGAATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCga                                                                                              <  SRR4302545.7348237/94‑3 (MQ=60)
   aaagggcatattcccccctggtgaaaggatgaaaGTGAAG‑CCCGCCACAATCAAGTCGTCCGGGGTCAGAACGATGGTCGAAGCGAGAGGCgat                                                                                             <  SRR4302545.9679254/60‑4 (MQ=60)
   AGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCgat                                                                                             >  SRR4302545.9026427/1‑91 (MQ=60)
   AGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCgat                                                                                             >  SRR4302545.5217979/1‑91 (MQ=60)
    gcagccgtgttccCCCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATG                                                                                            <  SRR4302545.10005467/81‑1 (MQ=60)
    gaagcaataTTATCCCGTTGGGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATG                                                                                            <  SRR4302545.6407738/85‑1 (MQ=60)
    accttgcatgtttcccccttgcaaaagggaagaagtaaacCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCgat                                                                                             <  SRR4302545.1122339/54‑4 (MQ=60)
     CAGGCATGTTATCCCCTTGTGAAGGGATTGAGCTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGTTGGTCGAAGGGAGAGGCgatgt                                                                                           >  SRR4302545.9137432/1‑89 (MQ=60)
     CAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGC                                                                                           >  SRR4302545.1820197/1‑94 (MQ=60)
     CAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGC                                                                                           >  SRR4302545.4150730/1‑94 (MQ=60)
     CAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGC                                                                                           >  SRR4302545.9017778/1‑94 (MQ=60)
      AGGCATTTTACCCCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCA                                                                                          <  SRR4302545.9093105/94‑1 (MQ=60)
      AGGCATGTTGTCCTCTTGTGAAGGTATAGGGGGTAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCA                                                                                          <  SRR4302545.3506497/94‑1 (MQ=60)
      AGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCA                                                                                          >  SRR4302545.2302901/1‑94 (MQ=60)
      AGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCA                                                                                          >  SRR4302545.9862982/1‑94 (MQ=60)
       GGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAG                                                                                         >  SRR4302545.6223205/1‑94 (MQ=60)
       GGCATGTTACCCCCTTGTGAGGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAG                                                                                         <  SRR4302545.3339127/94‑1 (MQ=60)
        ggcttctttcccctatgtgggggaacagggcagagcCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAG                                                                                         <  SRR4302545.4566131/58‑1 (MQ=60)
        GCATTTTACCCCCTTGTGAAGGGATAGAGGTAAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGC                                                                                        <  SRR4302545.990311/94‑1 (MQ=60)
        GCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCgatgcaga                                                                                        >  SRR4302545.4350364/1‑86 (MQ=60)
        GCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGC                                                                                        >  SRR4302545.10437511/1‑94 (MQ=60)
        GCATGTTACCCCCTGGTGAAGGGAAAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGC                                                                                        <  SRR4302545.6532865/94‑1 (MQ=60)
          ATGTTATCCCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAG                                                                                      >  SRR4302545.2230582/1‑94 (MQ=60)
          ATGTTATCCCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAG                                                                                      >  SRR4302545.10004611/1‑94 (MQ=60)
          ATGTTACCCCTTGGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAG                                                                                      <  SRR4302545.960897/94‑1 (MQ=60)
           TGTTATCCCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGG                                                                                     >  SRR4302545.6197524/1‑94 (MQ=60)
           TGTTATCCCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGG                                                                                     >  SRR4302545.2363182/1‑94 (MQ=60)
           TGTTATCCCCTGGAGAGGGGAAAGGGGTGAGG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGG                                                                                     <  SRR4302545.2707168/94‑1 (MQ=60)
            tttgcccccgggggaaaGGATAGAGGAGAAC‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGT                                                                                    <  SRR4302545.9447288/77‑1 (MQ=60)
            tttacccctttgaaaagggtaagcgggaAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGT                                                                                    <  SRR4302545.5962150/66‑1 (MQ=60)
            tttaccccttggggaggggaaAGAGGTGAGG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGT                                                                                    <  SRR4302545.7852216/73‑1 (MQ=60)
            TTTATCCCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGT                                                                                    <  SRR4302545.7225121/94‑1 (MQ=60)
            GTTATCCCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGT                                                                                    >  SRR4302545.10780658/1‑94 (MQ=60)
            GTTATCCCCTGGTGAAGGGAGAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGT                                                                                    <  SRR4302545.5080507/94‑1 (MQ=60)
             TTATCCCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTG                                                                                   >  SRR4302545.3025441/1‑94 (MQ=60)
              TACCCCCTTGTGAAGGGAGAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGC                                                                                  <  SRR4302545.5581299/94‑1 (MQ=60)
                TCCCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAA                                                                                >  SRR4302545.8907317/1‑94 (MQ=60)
                CCCCCTTGAGAAGGGATAGAGGTAAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAA                                                                                <  SRR4302545.6770286/94‑1 (MQ=60)
                 CCCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAA                                                                               >  SRR4302545.7726574/1‑94 (MQ=60)
                 CCCCTGGTAAAGGAAAAGAGGTGAAC‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAA                                                                               <  SRR4302545.2592993/94‑1 (MQ=60)
                  CCTTTGTGAGGGGATAGAGGTGACG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAAT                                                                              <  SRR4302545.3954591/94‑1 (MQ=60)
                  CCTTTGTGAGGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAAT                                                                              <  SRR4302545.6694845/94‑1 (MQ=60)
                  CCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAAT                                                                              >  SRR4302545.6663235/1‑94 (MQ=60)
                  CCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAAT                                                                              >  SRR4302545.2847838/1‑94 (MQ=60)
                   CCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATC                                                                             >  SRR4302545.7753864/1‑94 (MQ=60)
                     tggggagggaagAGAGGTAAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCA                                                                           <  SRR4302545.1886889/82‑1 (MQ=60)
                      ggagtggggaggtagggGAAG‑CCCGCCACAATCGCGGCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAG                                                                          <  SRR4302545.5529438/77‑1 (MQ=60)
                      TGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAG                                                                          >  SRR4302545.7817576/1‑94 (MQ=60)
                      TGTGAAGGGAATGA‑GTGAAGCCCCGCCAGAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAG                                                                          <  SRR4302545.4362453/94‑1 (MQ=60)
                       GTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGA                                                                         >  SRR4302545.1687001/1‑94 (MQ=60)
                        TGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGGGCAAATCCAGAT                                                                        >  SRR4302545.8379993/1‑94 (MQ=60)
                          AAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTG                                                                      >  SRR4302545.2848530/1‑94 (MQ=60)
                           AGGGATAGAGGTGAAG‑CCCGCCACGATCGCGTCGTCCGGGGTCTGAACGATGGGCGAAGCGAGAGGCGATGCAGCAGGGGCAAATCtggtttgt                                                                     >  SRR4302545.5287519/1‑86 (MQ=60)
                           AGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGT                                                                     >  SRR4302545.5179195/1‑94 (MQ=60)
                             GGAGAGAGGTGAAC‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTG                                                                   <  SRR4302545.2113879/94‑1 (MQ=60)
                              gagaaggtctgaacCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTG                                                                   <  SRR4302545.2674818/80‑1 (MQ=60)
                              gaagagaggtaaccCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTG                                                                   <  SRR4302545.2738300/80‑1 (MQ=60)
                              GATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGT                                                                  >  SRR4302545.6331141/1‑94 (MQ=60)
                              GATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGT                                                                  >  SRR4302545.10773040/1‑94 (MQ=60)
                              GATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGT                                                                  <  SRR4302545.515898/94‑1 (MQ=60)
                               aaggaggacAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTG                                                                 <  SRR4302545.8402287/85‑1 (MQ=60)
                               ATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTG                                                                 >  SRR4302545.6394331/1‑94 (MQ=60)
                                AAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGT                                                                <  SRR4302545.5503574/94‑1 (MQ=60)
                                 AGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTT                                                               <  SRR4302545.3470945/94‑1 (MQ=60)
                                 AGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTT                                                               >  SRR4302545.3625189/1‑94 (MQ=60)
                                 AGAGATGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTT                                                               >  SRR4302545.1577554/1‑94 (MQ=60)
                                  ggggaaagG‑CCCGCCACAATCGGGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTG                                                              <  SRR4302545.6373537/86‑1 (MQ=60)
                                  GAGGTGACG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTG                                                              <  SRR4302545.6066282/94‑1 (MQ=60)
                                   ggctaAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGT                                                             <  SRR4302545.1704762/89‑1 (MQ=60)
                                   gagaagacgaCCGCCACAACCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTG                                                              <  SRR4302545.6797283/84‑1 (MQ=60)
                                   GGGTGACG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGT                                                             <  SRR4302545.5878923/94‑1 (MQ=60)
                                   AGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGT                                                             >  SRR4302545.3276407/1‑94 (MQ=60)
                                   AGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGT                                                             >  SRR4302545.1767947/1‑94 (MQ=60)
                                    GGTGAGG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTT                                                            <  SRR4302545.307328/94‑1 (MQ=60)
                                    GGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTT                                                            <  SRR4302545.5926398/94‑1 (MQ=60)
                                    GGTGAAC‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTT                                                            <  SRR4302545.5926401/94‑1 (MQ=60)
                                    GGTGAAC‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTT                                                            <  SRR4302545.6477212/94‑1 (MQ=60)
                                     GTGAGG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTG                                                           <  SRR4302545.3357071/94‑1 (MQ=60)
                                     GTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTG                                                           <  SRR4302545.10412139/94‑1 (MQ=60)
                                     GTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTG                                                           <  SRR4302545.123980/94‑1 (MQ=60)
                                     GTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTG                                                           <  SRR4302545.8843333/94‑1 (MQ=60)
                                     ATGAAG‑CCCGCCACAAACGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTG                                                           <  SRR4302545.9953690/94‑1 (MQ=60)
                                       GAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCC                                                         >  SRR4302545.7492000/1‑94 (MQ=60)
                                        gagtccctCCACACTCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGCGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCC                                                         <  SRR4302545.9969952/86‑1 (MQ=60)
                                        AAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCA                                                        >  SRR4302545.2988407/1‑94 (MQ=60)
                                        AAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCA                                                        >  SRR4302545.8569327/1‑94 (MQ=60)
                                        AAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCA                                                        >  SRR4302545.219951/1‑94 (MQ=60)
                                        AAG‑CCCGCCAAAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCA                                                        >  SRR4302545.5775744/1‑94 (MQ=60)
                                         AG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCAT                                                       >  SRR4302545.453201/1‑94 (MQ=60)
                                         AG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCAT                                                       >  SRR4302545.7515232/1‑94 (MQ=60)
                                          gcCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTTCCAT                                                       <  SRR4302545.4987213/92‑1 (MQ=60)
                                          G‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATG                                                      >  SRR4302545.1614819/1‑94 (MQ=60)
                                            CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGG                                                     <  SRR4302545.4844082/94‑1 (MQ=60)
                                            CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGG                                                     >  SRR4302545.5394024/1‑94 (MQ=60)
                                             CCGCCACAATCGCGTCGTCCGGTGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGT                                                    <  SRR4302545.4748082/94‑1 (MQ=60)
                                             CCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAACTCCAGATTGTTGTGTTGTTGCCATGGT                                                    <  SRR4302545.6313689/94‑1 (MQ=60)
                                             CCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGT                                                    >  SRR4302545.2890055/1‑94 (MQ=60)
                                              CGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTA                                                   <  SRR4302545.7874144/94‑1 (MQ=60)
                                               GCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTAT                                                  <  SRR4302545.8578937/94‑1 (MQ=60)
                                                CCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATT                                                 >  SRR4302545.8120268/1‑94 (MQ=60)
                                                CCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATT                                                 <  SRR4302545.6576397/94‑1 (MQ=60)
                                                 CACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTC                                                >  SRR4302545.4051022/1‑94 (MQ=60)
                                                 CACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTC                                                >  SRR4302545.898452/1‑94 (MQ=60)
                                                 CACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTC                                                >  SRR4302545.1121877/1‑94 (MQ=60)
                                                 CACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTC                                                >  SRR4302545.6046908/1‑94 (MQ=60)
                                                  CCAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCT                                               <  SRR4302545.2503848/94‑1 (MQ=60)
                                                  ACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCT                                               >  SRR4302545.8686037/1‑94 (MQ=60)
                                                  ACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCT                                               <  SRR4302545.10516461/94‑1 (MQ=60)
                                                  ACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCT                                               >  SRR4302545.6176747/1‑94 (MQ=60)
                                                  ACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCT                                               >  SRR4302545.8393959/1‑94 (MQ=60)
                                                   CAATCGCGTCGTCCAGGGACTGAACGATCGGCGAAGGGAGAGGCGATGCCGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTC                                              >  SRR4302545.1781639/1‑94 (MQ=60)
                                                    AATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCC                                             >  SRR4302545.9199810/1‑94 (MQ=60)
                                                     ATCGCGTCGTGCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG                                            <  SRR4302545.3535618/94‑1 (MQ=60)
                                                        GCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑C                                        >  SRR4302545.3198139/1‑94 (MQ=60)
                                                        GCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑C                                        >  SRR4302545.9268944/1‑94 (MQ=60)
                                                         CGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CC                                       >  SRR4302545.4882201/1‑94 (MQ=60)
                                                         CGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CC                                       >  SRR4302545.4426813/1‑94 (MQ=60)
                                                          GTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCT                                      >  SRR4302545.3146245/1‑94 (MQ=60)
                                                            CGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCctt                                    >  SRR4302545.10119327/1‑91 (MQ=60)
                                                             GTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTA                                    <  SRR4302545.9525635/94‑1 (MQ=60)
                                                              TCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTAT                                   >  SRR4302545.9775800/1‑94 (MQ=60)
                                                              TCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCAATGGTATTCTCCGTACCCTTAT                                   >  SRR4302545.5064551/1‑94 (MQ=60)
                                                               CCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATA                                  >  SRR4302545.2368486/1‑94 (MQ=60)
                                                               CCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATA                                  <  SRR4302545.6254723/94‑1 (MQ=60)
                                                                CGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAA                                 >  SRR4302545.7433924/1‑94 (MQ=60)
                                                                CGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAA                                 >  SRR4302545.366726/1‑94 (MQ=60)
                                                                CGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAA                                 <  SRR4302545.2632264/94‑1 (MQ=60)
                                                                CGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAA                                 <  SRR4302545.7858605/94‑1 (MQ=60)
                                                                CGGGGGCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAA                                 <  SRR4302545.8506160/94‑1 (MQ=60)
                                                                 GGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAA                                >  SRR4302545.1766790/1‑94 (MQ=60)
                                                                 GGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAA                                >  SRR4302545.5311790/1‑94 (MQ=60)
                                                                  GGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAA                               <  SRR4302545.8081011/94‑1 (MQ=60)
                                                                    GTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCTGTACCCTTATAAAAAT                             >  SRR4302545.3978112/1‑94 (MQ=60)
                                                                     TCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATG                            >  SRR4302545.10815331/1‑94 (MQ=60)
                                                                     TCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATG                            <  SRR4302545.345381/94‑1 (MQ=60)
                                                                      CTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGT                           <  SRR4302545.9366051/94‑1 (MQ=60)
                                                                      CTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGT                           >  SRR4302545.7942123/1‑94 (MQ=60)
                                                                      CTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGT                           <  SRR4302545.2805103/94‑1 (MQ=60)
                                                                      CTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGT                           >  SRR4302545.4571940/1‑94 (MQ=60)
                                                                      CTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGT                           <  SRR4302545.3774559/94‑1 (MQ=60)
                                                                       TGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTT                          >  SRR4302545.8590785/1‑94 (MQ=60)
                                                                       TGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTT                          >  SRR4302545.1426928/1‑94 (MQ=60)
                                                                       TGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTT                          <  SRR4302545.5756496/94‑1 (MQ=60)
                                                                       TGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTT                          >  SRR4302545.4848377/1‑94 (MQ=60)
                                                                        GAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTG                         >  SRR4302545.10933734/1‑94 (MQ=60)
                                                                        GAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTG                         >  SRR4302545.3718046/1‑94 (MQ=60)
                                                                        GAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTG                         >  SRR4302545.89645/1‑94 (MQ=60)
                                                                        GAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTG                         >  SRR4302545.6944551/1‑94 (MQ=60)
                                                                        GAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTG                         <  SRR4302545.2049370/94‑1 (MQ=60)
                                                                        GAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTG                         >  SRR4302545.4683480/1‑94 (MQ=60)
                                                                        GAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTG                         >  SRR4302545.10583993/1‑94 (MQ=60)
                                                                         GACGATGGTGGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGC                        <  SRR4302545.4345576/94‑1 (MQ=60)
                                                                         AACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGC                        <  SRR4302545.10066038/94‑1 (MQ=60)
                                                                         AACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGC                        >  SRR4302545.7557944/1‑94 (MQ=60)
                                                                         AACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGC                        <  SRR4302545.883294/94‑1 (MQ=60)
                                                                          ACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCG                       >  SRR4302545.1224812/1‑94 (MQ=60)
                                                                          ACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCG                       <  SRR4302545.9880765/94‑1 (MQ=60)
                                                                           CGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGGGC                      >  SRR4302545.7697685/1‑94 (MQ=60)
                                                                           CGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGC                      <  SRR4302545.1867442/94‑1 (MQ=60)
                                                                            GATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCA                     <  SRR4302545.8454314/94‑1 (MQ=60)
                                                                             ATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAA                    >  SRR4302545.4179774/1‑94 (MQ=60)
                                                                             ATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAA                    <  SRR4302545.9788138/94‑1 (MQ=60)
                                                                              TGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAAT                   >  SRR4302545.2216459/1‑94 (MQ=60)
                                                                              TGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAAT                   >  SRR4302545.3879850/1‑94 (MQ=60)
                                                                              TGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAAT                   <  SRR4302545.4372977/94‑1 (MQ=60)
                                                                              TGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAAT                   <  SRR4302545.7545573/94‑1 (MQ=60)
                                                                               GGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATG                  <  SRR4302545.9615786/94‑1 (MQ=60)
                                                                               GGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATG                  <  SRR4302545.6380567/94‑1 (MQ=60)
                                                                                 TCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTT                >  SRR4302545.9822111/1‑94 (MQ=60)
                                                                                 TCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTT                <  SRR4302545.8327338/94‑1 (MQ=60)
                                                                                 TCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTT                >  SRR4302545.10193308/1‑94 (MQ=60)
                                                                                  CGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTA               >  SRR4302545.6812064/1‑94 (MQ=60)
                                                                                  CGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTA               >  SRR4302545.10870764/1‑94 (MQ=60)
                                                                                  CGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTA               <  SRR4302545.477755/94‑1 (MQ=60)
                                                                                  CGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGGATTCTACGTACCCTTATAAAAATGTTGCGCCATGTTA               >  SRR4302545.10879475/1‑94 (MQ=60)
                                                                                   GAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAA              >  SRR4302545.8759160/1‑94 (MQ=60)
                                                                                   GAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAA              <  SRR4302545.8607670/94‑1 (MQ=60)
                                                                                   GAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAA              <  SRR4302545.7048165/94‑1 (MQ=60)
                                                                                   GAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAA              >  SRR4302545.3369644/1‑94 (MQ=60)
                                                                                    AAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAAC             <  SRR4302545.8656629/94‑1 (MQ=60)
                                                                                     AGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACT            >  SRR4302545.8861626/1‑94 (MQ=60)
                                                                                     AGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACT            <  SRR4302545.7144559/94‑1 (MQ=60)
                                                                                       CGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTAT          <  SRR4302545.10547120/94‑1 (MQ=60)
                                                                                       CGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTAT          <  SRR4302545.8157586/94‑1 (MQ=60)
                                                                                       CGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTAT          <  SRR4302545.25214/94‑1 (MQ=60)
                                                                                       AGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTAT          <  SRR4302545.1399029/94‑1 (MQ=60)
                                                                                        GAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATA         >  SRR4302545.7645969/1‑94 (MQ=60)
                                                                                        GAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATA         >  SRR4302545.7361268/1‑94 (MQ=60)
                                                                                        GAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATA         >  SRR4302545.5027636/1‑94 (MQ=60)
                                                                                        GAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATA         <  SRR4302545.3690399/94‑1 (MQ=60)
                                                                                         AGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAG        >  SRR4302545.852159/1‑94 (MQ=60)
                                                                                          GAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGT       <  SRR4302545.2148120/94‑1 (MQ=60)
                                                                                           AGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTC      >  SRR4302545.10235680/1‑94 (MQ=60)
                                                                                           AGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTC      <  SRR4302545.49034/94‑1 (MQ=60)
                                                                                            GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCA     <  SRR4302545.5655558/94‑1 (MQ=60)
                                                                                              G‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCA  >  SRR4302545.5482015/1‑94 (MQ=60)
                                                                                              G‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCA  >  SRR4302545.1256341/1‑94 (MQ=60)
                                                                                              |                                                                                              
GTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAG‑CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCA  >  NC_000913/4013624‑4013809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑