Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,013,717	+G	coding (3/999 nt)	ysgA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,013,717	1	.	G	100.0%	684.1 / NA	199	M1M (ATG→ATC) †	ysgA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (104/95); total (104/95)

Only 100 of 215 total aligned reads displayed.

TAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCTTATAAAAATGTTGCGCAATGTTAACTATA  >  NC_000913/4013625‑4013802

tAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGc                                                                                         >  1:1084691/1‑93 (MQ=255)

 aaGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGcg                                                                                        >  2:1307478/1‑92 (MQ=255)

  aGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGcga                                                                                       >  2:4122278/1‑91 (MQ=255)

   gCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGcgat                                                                                      >  1:672270/1‑90 (MQ=255)

    cAGCCATGTTATCCCTTTGGGAAGGAATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGcgatg                                                                                     <  1:6022856/93‑5 (MQ=255)

      ggCTTGTTATCCCTTGGTGAGGGGATAGAGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATgca                                                                                   <  2:5627557/93‑1 (MQ=255)

      ggCATGTTATCCCCTTGTGAAGGGATAGAGGTGACGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATgca                                                                                   <  2:3664424/93‑1 (MQ=255)

      ggCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATgca                                                                                   >  2:2774396/1‑93 (MQ=255)

       gCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATgcag                                                                                  >  1:5934430/1‑93 (MQ=255)

        ctttttACCCCTTTGGGAGGGAATAGGGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATgcagc                                                                                 <  2:4175405/89‑1 (MQ=255)

         aTGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATgcagca                                                                                >  2:3264501/1‑93 (MQ=255)

          tGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGcagcag                                                                               >  2:1296928/1‑93 (MQ=255)

           tttACCCCTTTGTGAAGGGATAGAGGTGAGCCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAgg                                                                              <  2:1001208/92‑1 (MQ=255)

             tATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTg                                                                            >  1:4171060/1‑93 (MQ=255)

              aTCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGc                                                                           >  1:6357765/1‑93 (MQ=255)

                ccccTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCaa                                                                         >  1:4565540/1‑93 (MQ=255)

                ccccTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCaa                                                                         >  2:3607388/1‑93 (MQ=255)

                 cctttGTGAGGGAATAGAGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCaaa                                                                        <  1:4447933/90‑1 (MQ=255)

                 cccTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCaaa                                                                        >  2:3889619/1‑93 (MQ=255)

                   ctagtgAGGGGATAGGGGTGACGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATc                                                                      <  2:896569/90‑1 (MQ=255)

                   cTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCACATc                                                                      >  2:1164846/1‑93 (MQ=255)

                   cTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATc                                                                      >  2:5727470/1‑93 (MQ=255)

                   cTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATc                                                                      >  1:4797900/1‑93 (MQ=255)

                   cTTGAGAGGGAATAGAGCGGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATc                                                                      <  1:3432760/93‑1 (MQ=255)

                    ttGTGAAGGGATAGAGGTGAAGCCCGCCACAATCTCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATcc                                                                     >  1:4063577/1‑93 (MQ=255)

                    ttGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGGCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATcc                                                                     >  2:5250348/1‑93 (MQ=255)

                       tgAAGGGATAGAGGTGAGGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGa                                                                  <  1:2968990/93‑1 (MQ=255)

                        gAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCCGCAGGTGCAAATCCAGAt                                                                 >  2:974848/1‑93 (MQ=255)

                        gAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAt                                                                 >  1:3690997/1‑93 (MQ=255)

                           gggATAGAGTTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAttgt                                                              <  2:2882272/93‑1 (MQ=255)

                           gggATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGTTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAttgt                                                              >  1:4691484/1‑93 (MQ=255)

                            ggATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAttgtt                                                             <  2:5090693/93‑1 (MQ=255)

                              aTAGAGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATtgttgt                                                           <  2:1837523/93‑1 (MQ=255)

                               tAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATtgttgtg                                                          >  1:3921558/1‑93 (MQ=255)

                                agagGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATtgttgtgt                                                         >  1:5764863/1‑93 (MQ=255)

                                 gagGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATtgttgtgtt                                                        >  1:3978382/1‑93 (MQ=255)

                                 gagGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATtgttgtgtt                                                        >  2:712239/1‑93 (MQ=255)

                                   ggTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTgttgtgttgt                                                      >  2:4568601/1‑93 (MQ=255)

                                    gTGACGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTgttgtt                                                     <  2:218885/93‑1 (MQ=255)

                                    gTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTgttgtt                                                     >  2:1914029/1‑93 (MQ=255)

                                    gTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTgttgtt                                                     >  2:1588274/1‑93 (MQ=255)

                                    gTGAAGCCCGCCACAATCGCGTCGTCCGGGGACTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAACTCAAGATTGTTGAgttgtt                                                     >  2:2110941/1‑93 (MQ=255)

                                      gAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGc                                                   >  2:5465257/1‑93 (MQ=255)

                                      gAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGc                                                   <  1:5195993/93‑1 (MQ=255)

                                      gAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGc                                                   >  2:1480282/1‑93 (MQ=255)

                                       acgCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGcc                                                  <  1:3451331/91‑1 (MQ=255)

                                        aGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCa                                                 >  1:6135489/1‑93 (MQ=255)

                                          cccGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATg                                               <  1:3598807/93‑1 (MQ=255)

                                          cccGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATg                                               <  1:5359117/93‑1 (MQ=255)

                                           ccGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATgg                                              <  1:1400802/93‑1 (MQ=255)

                                           ccGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATgg                                              <  1:1973100/93‑1 (MQ=255)

                                            cGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGt                                             <  1:805377/93‑1 (MQ=255)

                                            cGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGt                                             >  2:3768144/1‑93 (MQ=255)

                                             gCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTa                                            <  2:3176340/93‑1 (MQ=255)

                                             gCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTa                                            >  2:1963994/1‑93 (MQ=255)

                                             gCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTa                                            <  2:5913573/93‑1 (MQ=255)

                                             gCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTa                                            <  2:6263174/93‑1 (MQ=255)

                                               cacaATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTAtt                                          >  1:5103857/1‑93 (MQ=255)

                                                acaATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTAttt                                         >  2:1603247/1‑92 (MQ=255)

                                                  aaTCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGCGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATtctc                                       >  2:3408508/1‑93 (MQ=255)

                                                  aaTCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATtctc                                       >  2:6066597/1‑93 (MQ=255)

                                                   aTCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTcc                                      <  2:1212609/93‑1 (MQ=255)

                                                   aTCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTcc                                      <  2:1084691/93‑1 (MQ=255)

                                                   aTCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTcc                                      >  1:3177141/1‑93 (MQ=255)

                                                    tCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCCTCGTATTCTCCg                                     >  2:2417159/1‑93 (MQ=255)

                                                    tCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCg                                     >  1:4468181/1‑93 (MQ=255)

                                                      gcgTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGtc                                   >  1:5341068/1‑92 (MQ=255)

                                                      gcgTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTa                                   <  2:470590/93‑1 (MQ=255)

                                                        gtcgtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑cc                                >  1:1282231/1‑93 (MQ=255)

                                                        gtcgtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑cc                                >  1:946661/1‑93 (MQ=255)

                                                              cGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTtata                           <  1:184882/93‑1 (MQ=255)

                                                              cGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTtata                           <  1:1495030/93‑1 (MQ=255)

                                                               ggggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATaa                          <  2:5262180/93‑1 (MQ=255)

                                                               ggggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATaa                          >  1:4868499/1‑93 (MQ=255)

                                                                gggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATaaa                         >  1:152299/1‑93 (MQ=255)

                                                                gggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTACTCTCCGTACCCTTATaaa                         >  1:5606313/1‑93 (MQ=255)

                                                                  gTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGTTTGTTGGGTTGTTGCCTTGGAATTCTCCTTA‑CCCTATTAAAAt                      >  1:2559824/1‑93 (MQ=255)

                                                                  gTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATaaaaa                       <  2:2599287/93‑1 (MQ=255)

                                                                  gTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATaaaaa                       >  1:3648623/1‑93 (MQ=255)

                                                                   tCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTcc                                      >  1:2477393/1‑77 (MQ=255)

                                                                   tCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAAt                      <  1:2736327/93‑1 (MQ=255)

                                                                     tGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGt                    >  1:4382796/1‑93 (MQ=255)

                                                                     tGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGt                    >  2:3482974/1‑93 (MQ=255)

                                                                      gAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGtt                   <  2:5433430/93‑1 (MQ=255)

                                                                       aaCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTg                  <  1:5852756/93‑1 (MQ=255)

                                                                       aaCGATGGACGAAGCGAGAGGCGATGAAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTg                  <  2:3589628/93‑1 (MQ=255)

                                                                        aCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTgc                 <  1:2228436/93‑1 (MQ=255)

                                                                         cGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTgcg                <  1:1722279/93‑1 (MQ=255)

                                                                          gATGGTCGAAGCGAGAGTCGATGCAGCAGGTGCAAATCCAGCTTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGCTgcgc               >  2:4925757/1‑93 (MQ=255)

                                                                               tCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGt          <  1:3890232/93‑1 (MQ=255)

                                                                               tCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGt          <  2:790641/93‑1 (MQ=255)

                                                                                cGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGtt         <  2:4382822/93‑1 (MQ=255)

                                                                                 gAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTa        >  1:22699/1‑93 (MQ=255)

                                                                                   aGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAAc      >  1:3401084/1‑93 (MQ=255)

                                                                                    gCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACt     >  2:1535942/1‑93 (MQ=255)

                                                                                     cGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACta    <  1:141377/93‑1 (MQ=255)

                                                                                      gagaGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTTTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACtat   <  1:5416281/93‑1 (MQ=255)

                                                                                      gagaGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACtat   >  2:4328069/1‑93 (MQ=255)

                                                                                      gagaGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACtat   >  2:1012960/1‑93 (MQ=255)

                                                                                       agagGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACtata  <  2:1778172/93‑1 (MQ=255)

TAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCTTATAAAAATGTTGCGCAATGTTAACTATA  >  NC_000913/4013625‑4013802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCA  >  NC_000913/4013624‑4013809

GTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGC                                                                                                >  SRR4302537.1092174/1‑94 (MQ=60)

GTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGC                                                                                                <  SRR4302537.6446298/94‑1 (MQ=60)

 TAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGC‑G                                                                                              >  SRR4302537.6112868/1‑94 (MQ=60)

  AAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCga                                                                                              >  SRR4302537.896127/1‑92 (MQ=60)

   AGCAGGCATGTTATCCCTTGGGGAAGGTATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCgat                                                                                             <  SRR4302537.1411067/94‑4 (MQ=60)

   AGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCgat                                                                                             >  SRR4302537.676872/1‑91 (MQ=60)

   AGCAGGCATGTTATCCCCTTGGGAGGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCgat                                                                                             <  SRR4302537.3673510/94‑4 (MQ=60)

    GCGGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATG                                                                                            <  SRR4302537.3131812/94‑1 (MQ=60)

     CAGCCATGTTATCCCTTTGGGAAGGAATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGC                                                                                           <  SRR4302537.6064235/94‑1 (MQ=60)

      AGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCA                                                                                          >  SRR4302537.2526787/1‑94 (MQ=60)

       GGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAG                                                                                         >  SRR4302537.5975156/1‑94 (MQ=60)

        gtatgttaccgcttgtggaggaaatgggggaGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGC                                                                                        <  SRR4302537.1312686/63‑1 (MQ=60)

          ATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAG                                                                                      >  SRR4302537.2718839/1‑94 (MQ=60)

          ATGTTACCCCCTTGGGAAGGAAGAGAGGCAATGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAG                                                                                      <  SRR4302537.4880287/94‑1 (MQ=60)

             ttaccccctggggaaggaaagggggcaacGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTG                                                                                   <  SRR4302537.2438356/65‑1 (MQ=60)

             TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTG                                                                                   >  SRR4302537.4200191/1‑94 (MQ=60)

             TTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTG                                                                                   >  SRR4302537.3711843/1‑94 (MQ=60)

              TATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCGGCAGGGGC                                                                                  >  SRR4302537.12797/1‑94 (MQ=60)

              TATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGC                                                                                  >  SRR4302537.6401153/1‑94 (MQ=60)

              TACCCCCTGGGGAAGGGATAGAGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGC                                                                                  <  SRR4302537.1615003/94‑1 (MQ=60)

               tcccctttgtaagggaaaaggggggagcCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCA                                                                                 <  SRR4302537.4215909/66‑1 (MQ=60)

               ATCCCCCTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCA                                                                                 >  SRR4302537.644518/1‑94 (MQ=60)

                TCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAA                                                                                >  SRR4302537.4597141/1‑94 (MQ=60)

                  CCTTTGTGAGGGAATAGAGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAAT                                                                              <  SRR4302537.4478648/94‑1 (MQ=60)

                   CCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATC                                                                             >  SRR4302537.4831006/1‑94 (MQ=60)

                   CCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATC                                                                             >  SRR4302537.5805071/1‑94 (MQ=60)

                    cttgagagggaatagagcgGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCC                                                                            <  SRR4302537.3457056/75‑1 (MQ=60)

                    CTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCTCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCC                                                                            >  SRR4302537.4091972/1‑94 (MQ=60)

                    CTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCC                                                                            >  SRR4302537.1004483/1‑94 (MQ=60)

                    CTTGTGAAGGGATAGAGGGGAAGCCCGCCACAATCGCGACGTCCGGGGACCGCACGATGGTAGAAGCGAGA‑GGCGATGCAGCGGGTGCAAcacc                                                                            >  SRR4302537.941024/1‑90 (MQ=50)

                    CTGGTGAGGGGAAAGAGGTGAGGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCC                                                                            <  SRR4302537.2284430/94‑1 (MQ=60)

                     TGGTGAGGGGATAGAGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCA                                                                           <  SRR4302537.4490939/94‑1 (MQ=60)

                      TGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAG                                                                          >  SRR4302537.3842768/1‑94 (MQ=60)

                        TGAAGGGATAGAGGTGAGGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGAT                                                                        <  SRR4302537.2989806/94‑1 (MQ=60)

                        TGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGAT                                                                        >  SRR4302537.3716969/1‑94 (MQ=60)

                         GAGGGGATAGGGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATT                                                                       <  SRR4302537.491693/94‑1 (MQ=60)

                           AGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGTTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGT                                                                     >  SRR4302537.4723931/1‑94 (MQ=60)

                            gggaaagaggaaagcCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTT                                                                    <  SRR4302537.5565716/79‑1 (MQ=60)

                            GGAATAGAGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTT                                                                    <  SRR4302537.2346761/94‑1 (MQ=60)

                             GGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTG                                                                   <  SRR4302537.1886371/94‑1 (MQ=60)

                              GATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGT                                                                  >  SRR4302537.3961029/1‑94 (MQ=60)

                               agagagtggaacCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTG                                                                 <  SRR4302537.2526665/82‑1 (MQ=60)

                               ATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTG                                                                 >  SRR4302537.3948965/1‑94 (MQ=60)

                               ATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTG                                                                 >  SRR4302537.3471851/1‑94 (MQ=60)

                               AAAGAGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTG                                                                 <  SRR4302537.4563935/94‑1 (MQ=60)

                                TAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGT                                                                >  SRR4302537.5804352/1‑94 (MQ=60)

                                AAGAGGTGAGGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGT                                                                <  SRR4302537.2820330/94‑1 (MQ=60)

                                 AGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTT                                                               >  SRR4302537.4006226/1‑94 (MQ=60)

                                    GGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTT                                                            <  SRR4302537.4339452/94‑1 (MQ=60)

                                     GTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTG                                                           >  SRR4302537.694939/1‑94 (MQ=60)

                                       GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCC                                                         <  SRR4302537.5231711/94‑1 (MQ=60)

                                       GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCC                                                         <  SRR4302537.5228687/94‑1 (MQ=60)

                                        ACGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCA                                                        <  SRR4302537.3475739/94‑1 (MQ=60)

                                        AAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCA                                                        >  SRR4302537.5998226/1‑94 (MQ=60)

                                        AAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCA                                                        >  SRR4302537.5500653/1‑94 (MQ=60)

                                        AAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCA                                                        >  SRR4302537.6177668/1‑94 (MQ=60)

                                           CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGG                                                     <  SRR4302537.3624174/94‑1 (MQ=60)

                                           CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGG                                                     <  SRR4302537.5395816/94‑1 (MQ=60)

                                            CCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGT                                                    <  SRR4302537.1410563/94‑1 (MQ=60)

                                            CCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGT                                                    <  SRR4302537.1986816/94‑1 (MQ=60)

                                            CCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGT                                                    >  SRR4302537.1318107/1‑94 (MQ=60)

                                            CCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGT                                                    <  SRR4302537.2879403/94‑1 (MQ=60)

                                             CGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTA                                                   <  SRR4302537.810908/94‑1 (MQ=60)

                                               CCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATT                                                 >  SRR4302537.5139030/1‑94 (MQ=60)

                                                CACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTC                                                <  SRR4302537.5670650/94‑1 (MQ=60)

                                                 ACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCGAATCCAGATTGTTGTGTTGTTGCCATGGTATTCT                                               <  SRR4302537.789961/94‑1 (MQ=60)

                                                 ACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCT                                               >  SRR4302537.3545129/1‑94 (MQ=60)

                                                   AATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCC                                             >  SRR4302537.5894291/1‑94 (MQ=60)

                                                   AATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCC                                             >  SRR4302537.3438738/1‑94 (MQ=60)

                                                   AATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCC                                             >  SRR4302537.3199471/1‑94 (MQ=60)

                                                    ATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG                                            <  SRR4302537.5033185/94‑1 (MQ=60)

                                                    ATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG                                            >  SRR4302537.4499082/1‑94 (MQ=60)

                                                    ATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGACTGTTGTGTTGTTGCCATGGTATTCTCCG                                            >  SRR4302537.6258890/1‑94 (MQ=60)

                                                     TCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGT                                           <  SRR4302537.4458145/94‑1 (MQ=60)

                                                      CGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTC                                          >  SRR4302537.5377674/1‑94 (MQ=60)

                                                        CGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CC                                       >  SRR4302537.953206/1‑94 (MQ=60)

                                                        CGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CC                                       >  SRR4302537.1291145/1‑94 (MQ=60)

                                                         GTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCC                                      >  SRR4302537.5286055/1‑94 (MQ=60)

                                                          TCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCCT                                     <  SRR4302537.1525233/94‑1 (MQ=60)

                                                              CCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATA                                  <  SRR4302537.6247153/94‑1 (MQ=60)

                                                               CGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAA                                 >  SRR4302537.4902093/1‑94 (MQ=60)

                                                               CGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAA                                 <  SRR4302537.5644769/94‑1 (MQ=60)

                                                               CGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAA                                 <  SRR4302537.186200/94‑1 (MQ=60)

                                                               CGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAA                                 <  SRR4302537.4099067/94‑1 (MQ=60)

                                                               CGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAA                                 <  SRR4302537.1505452/94‑1 (MQ=60)

                                                               CGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAA                                 <  SRR4302537.39388/94‑1 (MQ=60)

                                                                GGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAA                                >  SRR4302537.153387/1‑94 (MQ=60)

                                                                GGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTACTCTCCGTACCCTTATAAA                                >  SRR4302537.5644643/1‑94 (MQ=60)

                                                                  GGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGTTTGTTGGGTTGTTGCCTTGGAATTCTCCTTA‑CCCTATTAAAAT                             >  SRR4302537.2577763/1‑94 (MQ=57)

                                                                  GGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAA                              >  SRR4302537.3674309/1‑94 (MQ=60)

                                                                   GTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGacggaatagatcgga                             >  SRR4302537.2494808/1‑79 (MQ=60)

                                                                   GTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAAT                             <  SRR4302537.14541/94‑1 (MQ=60)

                                                                   GTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAAT                             <  SRR4302537.5421072/94‑1 (MQ=60)

                                                                    TCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATG                            <  SRR4302537.2755427/94‑1 (MQ=60)

                                                                     CTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGT                           >  SRR4302537.4413042/1‑94 (MQ=60)

                                                                     CTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGT                           >  SRR4302537.4638329/1‑94 (MQ=60)

                                                                        AACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGC                        >  SRR4302537.3343421/1‑94 (MQ=60)

                                                                        AACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGC                        <  SRR4302537.5892906/94‑1 (MQ=60)

                                                                         ACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCG                       <  SRR4302537.4087971/94‑1 (MQ=60)

                                                                         ACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCG                       <  SRR4302537.2244150/94‑1 (MQ=60)

                                                                          CGATGGTCGAAGCGAGAGGGG‑ATGCAGCGGGTGCAAACCCAGATTGTTGGTGTGTTGACATGGTATTCTCCGTACCCTTATCAAAATGTTGCGC                      >  SRR4302537.3157130/1‑94 (MQ=30)

                                                                          CGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGC                      >  SRR4302537.3157131/1‑94 (MQ=60)

                                                                          CGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGC                      <  SRR4302537.1734314/94‑1 (MQ=60)

                                                                             TGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAAT                   <  SRR4302537.3639197/94‑1 (MQ=60)

                                                                               GTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGT                 >  SRR4302537.1835146/1‑94 (MQ=60)

                                                                               GTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGT                 <  SRR4302537.4747519/94‑1 (MQ=60)

                                                                                TCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTT                <  SRR4302537.3917439/94‑1 (MQ=60)

                                                                                 CGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTA               <  SRR4302537.4211302/94‑1 (MQ=60)

                                                                                 CGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTA               >  SRR4302537.22874/1‑94 (MQ=60)

                                                                                   AAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAAC             >  SRR4302537.3425172/1‑94 (MQ=60)

                                                                                    AGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACT            <  SRR4302537.1835783/94‑1 (MQ=60)

                                                                                      CGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTAT          <  SRR4302537.5387298/94‑1 (MQ=60)

                                                                                      CGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTAT          <  SRR4302537.142381/94‑1 (MQ=60)

                                                                                       GAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTTTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATA         <  SRR4302537.5453208/94‑1 (MQ=60)

                                                                                        AGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAG        <  SRR4302537.2050303/94‑1 (MQ=60)

                                                                                         GA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGT       <  SRR4302537.980855/94‑1 (MQ=60)

                                                                                          A‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTC      <  SRR4302537.5228823/94‑1 (MQ=60)

                                                                                          A‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTC      <  SRR4302537.1987321/94‑1 (MQ=60)

                                                                                          A‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTC      <  SRR4302537.1795742/94‑1 (MQ=60)

                                                                                            GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCA     >  SRR4302537.6345007/1‑94 (MQ=60)

                                                                                            GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCA     <  SRR4302537.4876519/94‑1 (MQ=60)

                                                                                            GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCA     <  SRR4302537.2869334/94‑1 (MQ=60)

                                                                                            GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCA     <  SRR4302537.2007470/94‑1 (MQ=60)

                                                                                            GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCA     <  SRR4302537.1080361/94‑1 (MQ=60)

                                                                                              G‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCA  >  SRR4302537.687313/1‑94 (MQ=60)

GTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCA  >  NC_000913/4013624‑4013809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`