Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,019,860 Δ2 bp intergenic (+59/‑24) b3836 → / → b3838 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,019,8600C.99.6% 2309.0 / ‑1.2 508intergenic (+59/‑25)b3836/b3838hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base . (254/252);  total (254/254)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.62e-01
*NC_0009134,019,8610C.99.8% 2312.1 / ‑3.7 508intergenic (+60/‑24)b3836/b3838hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base . (254/252);  total (254/254)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.96e-01

Only 100 of 518 total aligned reads displayed.

GAAGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGCCGCCTTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACGGT  >  NC_000913/4019774‑4019943
                                                                                      ||                                                                                  
gAAGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTgggg                                                                            >  1:1709570/1‑94 (MQ=255)
 aaGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGc                                                                           >  1:4896322/1‑94 (MQ=255)
 aaGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGc                                                                           >  1:1422869/1‑94 (MQ=255)
  aGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGcc                                                                          <  2:7877224/94‑1 (MQ=255)
  aGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGcc                                                                          <  1:2860325/94‑1 (MQ=255)
   gcgcCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCg                                                                         >  2:9680348/1‑94 (MQ=255)
   gcgcCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCCATTGGTGTTCATCATCGGCCTCG‑‑TCCTTCTGGGGCCg                                                                         <  1:6863469/94‑1 (MQ=255)
        aCGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACg                                                                    >  2:4079181/1‑94 (MQ=255)
        aCGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACg                                                                    >  2:3617553/1‑94 (MQ=255)
        aCGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACg                                                                    <  2:4637240/94‑1 (MQ=255)
          gATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGAc                                                                  >  1:5088695/1‑94 (MQ=255)
          gATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGAc                                                                  >  1:8037341/1‑94 (MQ=255)
           aTAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACt                                                                 <  1:1053688/94‑1 (MQ=255)
           aTAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGCCG‑‑TTCTGGGGCGGCAACgctt                                                                 >  1:8348379/1‑91 (MQ=255)
           aTAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCG‑‑TCATTCTGGGGCCGCAACGACt                                                                 <  1:2625604/94‑1 (MQ=255)
            tAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTg                                                                <  2:10340259/94‑1 (MQ=255)
              aaGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGcc                                                              >  1:7062048/1‑94 (MQ=255)
               atagCAGGTGTAATCCGTTTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCt                                                             <  1:2478089/92‑1 (MQ=255)
                 agCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCtgt                                                           <  2:8887517/94‑1 (MQ=255)
                 agCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCtgt                                                           <  1:9131507/94‑1 (MQ=255)
                 agCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCtgg                                                           >  2:162366/1‑93 (MQ=255)
                 agCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCtgg                                                           >  2:513862/1‑93 (MQ=255)
                   cAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTgg                                                         <  2:3840860/94‑1 (MQ=255)
                    aGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGc                                                        >  2:6135283/1‑94 (MQ=255)
                       tgtAATCCGTGTTTGATTTCGGTTTTAGCGAACTGCTCTTGGTGTTCATCATCGGCATCGTCG‑‑TTCTAGGCACGCAACGACTGCCTGTggtggt                                                     >  1:7602016/1‑94 (MQ=255)
                       tgtAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGt                                                     >  2:2432471/1‑94 (MQ=255)
                        gtAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTa                                                    <  1:10605612/94‑1 (MQ=255)
                         tAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTaa                                                   >  2:9240832/1‑94 (MQ=255)
                          aaTCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTaaa                                                  >  2:4842386/1‑94 (MQ=255)
                          aaTCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTaaa                                                  <  1:221794/94‑1 (MQ=255)
                           aTCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTaaca                                                 >  2:8131071/1‑92 (MQ=255)
                           aTCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTaaaa                                                 >  2:175735/1‑94 (MQ=255)
                            tCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTaaaaa                                                <  1:5167901/94‑1 (MQ=255)
                             ccGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAAc                                               >  2:2774582/1‑94 (MQ=255)
                             ccGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAAc                                               >  1:8765539/1‑94 (MQ=255)
                                tgtTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGt                                            >  2:2071498/1‑94 (MQ=255)
                                 gtTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTa                                           <  2:1122855/94‑1 (MQ=255)
                                 gtTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTa                                           <  2:2891732/94‑1 (MQ=255)
                                 gtTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTa                                           <  1:1918593/94‑1 (MQ=255)
                                 gtTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTa                                           <  1:5016002/94‑1 (MQ=255)
                                 gtTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTa                                           >  2:9473887/1‑94 (MQ=255)
                                 gtTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTa                                           <  2:1964648/94‑1 (MQ=255)
                                 gtTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTa                                           <  1:9027056/94‑1 (MQ=255)
                                  tttGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAg                                          <  2:431164/94‑1 (MQ=255)
                                   ttGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGc                                         <  2:7649471/94‑1 (MQ=255)
                                    tGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTACAAACGGTAGCg                                        >  2:10549745/1‑94 (MQ=255)
                                     gATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCgg                                       <  2:4473750/94‑1 (MQ=255)
                                     gATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCgg                                       >  2:7510809/1‑94 (MQ=255)
                                      atatCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCggg                                      >  1:10104852/1‑94 (MQ=255)
                                      atatCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCggg                                      >  2:6798351/1‑94 (MQ=255)
                                      atatCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCggg                                      >  1:3222450/1‑94 (MQ=255)
                                      atatCAGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCggg                                      <  2:1264175/94‑1 (MQ=255)
                                        atCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCt                                    <  2:9065091/94‑1 (MQ=255)
                                        atCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCt                                    >  1:395332/1‑94 (MQ=255)
                                        atCGGTGTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCt                                    <  2:532773/94‑1 (MQ=255)
                                          cGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTgg                                  <  1:6091777/94‑1 (MQ=255)
                                          cGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTgg                                  >  2:8268694/1‑94 (MQ=255)
                                           ggTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGa                                 >  2:10031054/1‑94 (MQ=255)
                                           ggTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGa                                 >  2:400569/1‑94 (MQ=255)
                                            gTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGAt                                >  1:6829408/1‑94 (MQ=255)
                                            gTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGAt                                >  2:3043433/1‑94 (MQ=255)
                                            gTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGAt                                <  1:2961165/94‑1 (MQ=255)
                                            gTTTTAGCGAACTGCTATTGGTGTTCATCAGCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGAt                                >  1:6529308/1‑94 (MQ=255)
                                              tttAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACCGTAGCGGGCTGGATTc                              >  1:7794467/1‑94 (MQ=255)
                                               ttAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTcg                             <  1:9596964/94‑1 (MQ=255)
                                               ttAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTcg                             >  1:5799896/1‑94 (MQ=255)
                                                tAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTcgc                            >  1:7862315/1‑94 (MQ=255)
                                                tAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTcgc                            >  1:5583171/1‑94 (MQ=255)
                                                tAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTcgc                            <  2:7737904/94‑1 (MQ=255)
                                                 aGCGAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTcgcg                           >  1:1025434/1‑94 (MQ=255)
                                                   ctaaCTGCTATTGTTGTTCATCATCGGCCTCGTCG‑‑TTCTGCGGCCTCAACGACTGCCTTTGGCGGTAAAAACGGTAGCGGGCTGGATTcgcgcg                         <  2:7321820/92‑1 (MQ=255)
                                                   cGAACTGCTATTGGTGTTGATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTcgcgcg                         >  1:4413330/1‑94 (MQ=255)
                                                    gAACTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCgcgt                        <  2:872407/94‑1 (MQ=255)
                                                     aaCTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCgcgtt                       <  2:8419534/94‑1 (MQ=255)
                                                      aCTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCgcgttg                      <  1:9781363/94‑1 (MQ=255)
                                                      aCTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCgcgttg                      <  2:5552415/94‑1 (MQ=255)
                                                       cTGCTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCgcgttgc                     <  1:8648333/94‑1 (MQ=255)
                                                          cTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCgcgttgcgtt                  >  1:2097024/1‑94 (MQ=255)
                                                          cTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCgcgttgcgtt                  >  1:4256750/1‑94 (MQ=255)
                                                          cTATTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCATGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCgcgttgcgtt                  <  2:6449022/94‑1 (MQ=255)
                                                            aTTGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCa                >  1:9302425/1‑94 (MQ=255)
                                                              tGGTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACt              <  2:5923479/94‑1 (MQ=255)
                                                               ggTGTTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTg             >  2:6636049/1‑94 (MQ=255)
                                                                gtgtTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTgg            >  1:5906864/1‑94 (MQ=255)
                                                                gtgtTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTgg            <  2:6925163/94‑1 (MQ=255)
                                                                gtgtTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTgg            >  1:3512394/1‑94 (MQ=255)
                                                                gtgtTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTgg            >  2:4965403/1‑94 (MQ=255)
                                                                gtgtTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTgg            <  1:2430008/94‑1 (MQ=255)
                                                                gtgtTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTgg            <  1:4073664/94‑1 (MQ=255)
                                                                gtgtTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCCTGGCGTTCACTgc            >  2:7988198/1‑93 (MQ=255)
                                                                 tgtTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGc           >  1:5415992/1‑94 (MQ=255)
                                                                  gtTCATCATCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCg          <  2:265225/94‑1 (MQ=255)
                                                                    tcatcaTCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGAc        >  1:4659112/1‑94 (MQ=255)
                                                                    tcatcaTCGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGAc        >  1:1794096/1‑94 (MQ=255)
                                                                       tcatcGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGTCTGGATTCGCGCGTTGCGTTCACTGGCGACAAc     <  1:1208472/94‑1 (MQ=255)
                                                                       tcatcGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAAc     <  1:10446191/94‑1 (MQ=255)
                                                                         ctcGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACgg   <  2:3363932/93‑1 (MQ=255)
                                                                          tcGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGTTGGATTCGCGCGTTGCGTTCACTGGCGACAACGGt  <  1:9427979/94‑1 (MQ=255)
                                                                          tcGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACggg  >  1:7189282/1‑93 (MQ=255)
                                                                          tcGGCCTCGTCG‑‑TTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACGGt  <  2:6232249/94‑1 (MQ=255)
                                                                                      ||                                                                                  
GAAGCGCCACGATAAAGAGCAGGTGTAATCCGTGTTTGATATCGGTTTTAGCGAACTGCTATTGGTGTTCATCATCGGCCTCGCCGCCTTCTGGGGCCGCAACGACTGCCTGTGGCGGTAAAAACGGTAGCGGGCTGGATTCGCGCGTTGCGTTCACTGGCGACAACGGT  >  NC_000913/4019774‑4019943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 322 total aligned reads displayed.
TTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACCGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATC  >  NC_000913/4021591‑4021777
                                                                                             |                                                                                             
TTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACG                                                                                               <  SRR4302545.6811781/94‑1 (MQ=60)
TTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACG                                                                                               <  SRR4302545.7001626/94‑1 (MQ=60)
TTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACG                                                                                               <  SRR4302545.48861/94‑1 (MQ=60)
TTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACG                                                                                               >  SRR4302545.7160717/1‑94 (MQ=60)
TTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACG                                                                                               <  SRR4302545.7664719/94‑1 (MQ=60)
TTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACG                                                                                               <  SRR4302545.3981528/94‑1 (MQ=60)
  CTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGAC                                                                                            >  SRR4302545.2872611/1‑94 (MQ=60)
  CTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGAC                                                                                            >  SRR4302545.716628/1‑94 (MQ=60)
  CTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGAC                                                                                            >  SRR4302545.3934308/1‑94 (MQ=60)
  CTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGAC                                                                                            <  SRR4302545.9480992/94‑1 (MQ=60)
  CTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGAC                                                                                            <  SRR4302545.5806453/94‑1 (MQ=60)
  CTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGAC                                                                                            <  SRR4302545.4695808/94‑1 (MQ=60)
   TTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACG                                                                                           <  SRR4302545.6074338/94‑1 (MQ=60)
   TTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACG                                                                                           <  SRR4302545.6497301/94‑1 (MQ=60)
   TTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACG                                                                                           >  SRR4302545.2997439/1‑94 (MQ=60)
   TTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACG                                                                                           >  SRR4302545.3161969/1‑94 (MQ=60)
    TCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTCCCGGTTGGGTTTGCGATGAA‑CGACGC                                                                                          <  SRR4302545.8626005/94‑1 (MQ=60)
    TCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGC                                                                                          >  SRR4302545.4056276/1‑94 (MQ=60)
    TCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGC                                                                                          >  SRR4302545.9769374/1‑94 (MQ=60)
    TCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGC                                                                                          <  SRR4302545.4236755/94‑1 (MQ=60)
     CATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCG                                                                                         >  SRR4302545.6062467/1‑94 (MQ=60)
     CATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCG                                                                                         >  SRR4302545.2705969/1‑94 (MQ=60)
     CATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCG                                                                                         <  SRR4302545.9097754/94‑1 (MQ=60)
       TTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGTGATGAA‑CGACGCGGA                                                                                       >  SRR4302545.5106636/1‑94 (MQ=60)
         GCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACT                                                                                     <  SRR4302545.3898935/94‑1 (MQ=60)
          CTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTG                                                                                    >  SRR4302545.719329/1‑94 (MQ=60)
          CTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTG                                                                                    >  SRR4302545.1004474/1‑94 (MQ=60)
           TTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGG                                                                                   <  SRR4302545.8968997/94‑1 (MQ=60)
            TTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGA                                                                                  <  SRR4302545.5047430/94‑1 (MQ=60)
            TTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGA                                                                                  <  SRR4302545.10187268/94‑1 (MQ=60)
            TTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGA                                                                                  <  SRR4302545.6268739/94‑1 (MQ=60)
            TGACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGA                                                                                  <  SRR4302545.1595850/94‑1 (MQ=60)
             TACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAG                                                                                 <  SRR4302545.2460868/94‑1 (MQ=60)
             TACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAG                                                                                 <  SRR4302545.3608823/94‑1 (MQ=60)
              ACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CAACGCGGACTGGAGC                                                                                >  SRR4302545.8166111/1‑94 (MQ=60)
               CCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCT                                                                               <  SRR4302545.6815110/94‑1 (MQ=60)
               CCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCT                                                                               <  SRR4302545.10877483/94‑1 (MQ=60)
               CCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCT                                                                               <  SRR4302545.57144/94‑1 (MQ=60)
               CCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCT                                                                               <  SRR4302545.10255157/94‑1 (MQ=60)
               CCGGAACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGGTGGGTTTGCGATGAA‑CGCCGCGGACTGGAGCT                                                                               >  SRR4302545.5458382/1‑94 (MQ=60)
                CGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTG                                                                              >  SRR4302545.7158143/1‑94 (MQ=60)
                CGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTG                                                                              <  SRR4302545.5017729/94‑1 (MQ=60)
                CGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTG                                                                              <  SRR4302545.3849058/94‑1 (MQ=60)
                CGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTG                                                                              >  SRR4302545.595997/1‑94 (MQ=60)
                CGGCACACGCGAAGAGATGCAGGCGTGCGCGGCGCATGGAATTTATATCGGCTTTATCGGTTGGGTTTGCGACGAA‑CGCCGCGGACTGGGGCTG                                                                              >  SRR4302545.8866243/1‑94 (MQ=60)
                 GGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGC                                                                             <  SRR4302545.2357068/94‑1 (MQ=60)
                  GCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGATTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCG                                                                            <  SRR4302545.1204129/94‑1 (MQ=60)
                    ACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGGGCTGCGGG                                                                          >  SRR4302545.2207998/1‑94 (MQ=60)
                     CACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGA                                                                         >  SRR4302545.4421896/1‑94 (MQ=60)
                     CACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGA                                                                         >  SRR4302545.7144445/1‑94 (MQ=60)
                     CACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGA                                                                         >  SRR4302545.4421898/1‑94 (MQ=60)
                       CGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAAC                                                                       >  SRR4302545.2821376/1‑94 (MQ=60)
                        GCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACT                                                                      >  SRR4302545.9764331/1‑94 (MQ=60)
                        GCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACT                                                                      <  SRR4302545.10048031/94‑1 (MQ=60)
                        GCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACT                                                                      <  SRR4302545.3402824/94‑1 (MQ=60)
                         CGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTT                                                                     >  SRR4302545.2259825/1‑94 (MQ=60)
                         CGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCCGGAACTT                                                                     >  SRR4302545.4243772/1‑94 (MQ=60)
                          GAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTT                                                                    >  SRR4302545.3801728/1‑94 (MQ=60)
                          GAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTT                                                                    <  SRR4302545.8091132/94‑1 (MQ=60)
                          GAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTT                                                                    <  SRR4302545.8568961/94‑1 (MQ=60)
                           AAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTT                                                                   <  SRR4302545.4761751/94‑1 (MQ=60)
                           AAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTT                                                                   <  SRR4302545.10001423/94‑1 (MQ=60)
                           AAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTT                                                                   <  SRR4302545.5771460/94‑1 (MQ=60)
                           AAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTT                                                                   >  SRR4302545.9265392/1‑94 (MQ=60)
                            AGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTG                                                                  <  SRR4302545.4497370/94‑1 (MQ=60)
                            AGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTG                                                                  <  SRR4302545.883069/94‑1 (MQ=60)
                            AGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTG                                                                  <  SRR4302545.1680545/94‑1 (MQ=60)
                            AGAGATGAAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGGTGGGTTTGGGATGAA‑CGCCGCGGACTGGAGCTGCGGGgagttatg                                                                  >  SRR4302545.3393578/1‑86 (MQ=60)
                             GAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGGTGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGC                                                                 >  SRR4302545.6878350/1‑94 (MQ=60)
                             GAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGC                                                                 <  SRR4302545.8487999/94‑1 (MQ=60)
                              AGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCC                                                                <  SRR4302545.5119841/94‑1 (MQ=60)
                              AGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCC                                                                <  SRR4302545.10076401/94‑1 (MQ=60)
                              AGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCC                                                                <  SRR4302545.9032568/94‑1 (MQ=60)
                              AGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCC                                                                >  SRR4302545.748801/1‑94 (MQ=60)
                              AGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCC                                                                >  SRR4302545.2092721/1‑94 (MQ=60)
                              AGATGCAGGCGAGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCG                                                                >  SRR4302545.7585523/1‑94 (MQ=60)
                                ATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGT                                                              >  SRR4302545.7729062/1‑94 (MQ=60)
                                ATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGT                                                              <  SRR4302545.8315646/94‑1 (MQ=60)
                                ATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGT                                                              >  SRR4302545.7509081/1‑94 (MQ=60)
                                 TGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGATCTTTTGCCGTT                                                             <  SRR4302545.7051454/94‑1 (MQ=60)
                                 TGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTT                                                             <  SRR4302545.482648/94‑1 (MQ=60)
                                   CAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGA                                                           >  SRR4302545.9803420/1‑94 (MQ=60)
                                    AGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGAT                                                          >  SRR4302545.1817569/1‑94 (MQ=60)
                                    AGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGAT                                                          >  SRR4302545.6581422/1‑94 (MQ=60)
                                     GGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATT                                                         <  SRR4302545.3348729/94‑1 (MQ=60)
                                     GGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATT                                                         <  SRR4302545.4596874/94‑1 (MQ=60)
                                     GGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATT                                                         <  SRR4302545.10811131/94‑1 (MQ=60)
                                      GCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTTCGGGAACTTTTGCCGTTGATTC                                                        <  SRR4302545.6953516/94‑1 (MQ=60)
                                      GCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTC                                                        <  SRR4302545.1442161/94‑1 (MQ=60)
                                       CGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCC                                                       <  SRR4302545.2680982/94‑1 (MQ=60)
                                       CGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCC                                                       >  SRR4302545.153976/1‑94 (MQ=60)
                                       CGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCC                                                       <  SRR4302545.621323/94‑1 (MQ=60)
                                        GTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCG                                                      >  SRR4302545.115893/1‑94 (MQ=60)
                                        GTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCG                                                      >  SRR4302545.1560996/1‑94 (MQ=60)
                                        GTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCG                                                      >  SRR4302545.5061612/1‑94 (MQ=60)
                                        GTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCG                                                      <  SRR4302545.240178/94‑1 (MQ=60)
                                        GTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGGTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCG                                                      >  SRR4302545.3948827/1‑94 (MQ=60)
                                         TGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGG                                                     <  SRR4302545.5731619/94‑1 (MQ=60)
                                          GCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGC                                                    >  SRR4302545.1653078/1‑94 (MQ=60)
                                           CGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCG                                                   >  SRR4302545.8739118/1‑94 (MQ=60)
                                           CGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCG                                                   >  SRR4302545.10154609/1‑94 (MQ=60)
                                           CGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCG                                                   <  SRR4302545.9787972/94‑1 (MQ=60)
                                               GCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAA                                               >  SRR4302545.2548509/1‑94 (MQ=60)
                                                CGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAA                                              >  SRR4302545.7746047/1‑94 (MQ=60)
                                                CGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAA                                              <  SRR4302545.10787340/94‑1 (MQ=60)
                                                CGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAA                                              <  SRR4302545.342076/94‑1 (MQ=60)
                                                  CATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAAT                                            >  SRR4302545.6049756/1‑94 (MQ=60)
                                                  CATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAAT                                            >  SRR4302545.3507295/1‑94 (MQ=60)
                                                   ATGTAATGTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATT                                           <  SRR4302545.10005543/94‑1 (MQ=60)
                                                   ATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATT                                           >  SRR4302545.6604787/1‑94 (MQ=60)
                                                   ATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATT                                           <  SRR4302545.3508589/94‑1 (MQ=60)
                                                   ATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATT                                           <  SRR4302545.3508595/94‑1 (MQ=60)
                                                   ATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATT                                           >  SRR4302545.5897964/1‑94 (MQ=60)
                                                    TGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTA                                          >  SRR4302545.3225233/1‑94 (MQ=60)
                                                    TGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTA                                          <  SRR4302545.8255157/94‑1 (MQ=60)
                                                     GGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTAC                                         >  SRR4302545.2357047/1‑94 (MQ=60)
                                                     GGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTAC                                         <  SRR4302545.1950709/94‑1 (MQ=60)
                                                     GGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTAC                                         >  SRR4302545.6373400/1‑94 (MQ=60)
                                                     GGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTAC                                         >  SRR4302545.6373399/1‑94 (MQ=60)
                                                     GGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTAC                                         >  SRR4302545.4188016/1‑94 (MQ=60)
                                                     GGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTAC                                         >  SRR4302545.5884741/1‑94 (MQ=60)
                                                      GAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACT                                        >  SRR4302545.7820744/1‑94 (MQ=60)
                                                      GAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCATTGATTCCGGCGGAAAAATTACT                                        <  SRR4302545.3987953/94‑1 (MQ=60)
                                                      GAATGTATGTCGGCGTTGCCGGTTGGGTTTGCGATGAA‑TGACGCGGGCTGGAGCTGCTGGAACTTTTGCCGTTGATTCCTGCGGAAAAATTAGT                                        <  SRR4302545.6651183/94‑1 (MQ=48)
                                                       AATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTG                                       <  SRR4302545.4468381/94‑1 (MQ=60)
                                                       AATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTG                                       >  SRR4302545.9549800/1‑94 (MQ=60)
                                                       AATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTG                                       <  SRR4302545.8785122/94‑1 (MQ=60)
                                                       AATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGAAAAAATTACTG                                       <  SRR4302545.7899328/94‑1 (MQ=60)
                                                        ATTTTTATCGGCACTTGCGGTTGGGTTGGCGATGAA‑CGACGCAGACTGGAGCTGCGCGAGCTTTTCCCGTTGATTCCGGCGGAAAAATTACTGA                                      <  SRR4302545.7767469/94‑1 (MQ=44)
                                                         TTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGTGACCTTTTGCCGTTGATTCCGGCGGAAAAATTACTGAT                                     <  SRR4302545.2079061/94‑1 (MQ=60)
                                                         TTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGAT                                     <  SRR4302545.2006029/94‑1 (MQ=60)
                                                          TTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGCTTCCGGCGGAAAAATTACTGATC                                    >  SRR4302545.10681424/1‑94 (MQ=60)
                                                          TTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATC                                    <  SRR4302545.1472302/94‑1 (MQ=60)
                                                          TTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATC                                    <  SRR4302545.4705070/94‑1 (MQ=60)
                                                           TATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCG                                   >  SRR4302545.1243782/1‑94 (MQ=60)
                                                           TATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCG                                   <  SRR4302545.6292296/94‑1 (MQ=60)
                                                           TATATCGGCATTACCGGTTGGGTTTGAGCTGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGCTCG                                   <  SRR4302545.6292295/94‑1 (MQ=60)
                                                            ATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGA                                  >  SRR4302545.8633634/1‑94 (MQ=60)
                                                            ATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGA                                  >  SRR4302545.1789938/1‑94 (MQ=60)
                                                            ATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGA                                  <  SRR4302545.7445237/94‑1 (MQ=60)
                                                            ATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGA                                  <  SRR4302545.2960669/94‑1 (MQ=60)
                                                            ATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGA                                  >  SRR4302545.6320966/1‑94 (MQ=60)
                                                             TATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAA                                 >  SRR4302545.10486804/1‑94 (MQ=60)
                                                             TATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAA                                 >  SRR4302545.8496136/1‑94 (MQ=60)
                                                             TATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAA                                 >  SRR4302545.8242043/1‑94 (MQ=60)
                                                              ATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAA                                >  SRR4302545.5722206/1‑94 (MQ=60)
                                                               TCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAAC                               <  SRR4302545.6603704/94‑1 (MQ=60)
                                                                CGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACT                              <  SRR4302545.7167882/94‑1 (MQ=60)
                                                                 GGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTG                             <  SRR4302545.7141299/94‑1 (MQ=60)
                                                                 GGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTG                             >  SRR4302545.5029700/1‑94 (MQ=60)
                                                                  GCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGA                            <  SRR4302545.7573671/94‑1 (MQ=60)
                                                                  GCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGA                            >  SRR4302545.5502847/1‑94 (MQ=60)
                                                                   CATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGAT                           <  SRR4302545.5298944/94‑1 (MQ=60)
                                                                   CATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGAT                           >  SRR4302545.7353502/1‑94 (MQ=60)
                                                                   CATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGAT                           >  SRR4302545.9495741/1‑94 (MQ=60)
                                                                   CATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGAT                           <  SRR4302545.10927074/94‑1 (MQ=60)
                                                                    ATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGTTGCGGGAACTTTTGCCGTTGATTCCGGCCGAACAATTCCTGATCGcaaatcatg                          >  SRR4302545.3259133/1‑85 (MQ=60)
                                                                    ATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATG                          <  SRR4302545.5010312/94‑1 (MQ=60)
                                                                    ATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATG                          >  SRR4302545.4006622/1‑94 (MQ=60)
                                                                    ATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATG                          >  SRR4302545.4649425/1‑94 (MQ=60)
                                                                     TTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGACCTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGC                         <  SRR4302545.5338062/94‑1 (MQ=60)
                                                                     TTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGC                         >  SRR4302545.2950175/1‑94 (MQ=60)
                                                                      TACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCG                        <  SRR4302545.2905377/94‑1 (MQ=60)
                                                                      TACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCG                        >  SRR4302545.2618758/1‑94 (MQ=60)
                                                                       ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGC                       >  SRR4302545.504053/1‑94 (MQ=60)
                                                                       ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGC                       <  SRR4302545.5949337/94‑1 (MQ=60)
                                                                       ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGC                       >  SRR4302545.6555083/1‑94 (MQ=60)
                                                                       ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGC                       >  SRR4302545.8629557/1‑94 (MQ=60)
                                                                        CCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCC                      >  SRR4302545.8709048/1‑94 (MQ=60)
                                                                        CCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCC                      >  SRR4302545.6924928/1‑94 (MQ=60)
                                                                         CGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCG                     <  SRR4302545.4467842/94‑1 (MQ=60)
                                                                         CGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCG                     >  SRR4302545.10112198/1‑94 (MQ=60)
                                                                         CGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCG                     <  SRR4302545.6886274/94‑1 (MQ=60)
                                                                          GGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGT                    >  SRR4302545.4312245/1‑94 (MQ=60)
                                                                           GTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTA                   >  SRR4302545.7385711/1‑94 (MQ=60)
                                                                             TGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATC                 >  SRR4302545.5692549/1‑94 (MQ=60)
                                                                             TGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATC                 <  SRR4302545.9071350/94‑1 (MQ=60)
                                                                             TGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATC                 <  SRR4302545.9040173/94‑1 (MQ=60)
                                                                             TGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATC                 >  SRR4302545.4124195/1‑94 (MQ=60)
                                                                              GGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAAGTACTGATCGAAGCTGATGCGCCGTATCT                <  SRR4302545.1860025/94‑1 (MQ=60)
                                                                               GGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTG               >  SRR4302545.10610188/1‑94 (MQ=60)
                                                                               GGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTC               >  SRR4302545.1942500/1‑94 (MQ=60)
                                                                                GTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGT              >  SRR4302545.2230242/1‑94 (MQ=60)
                                                                                GTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGC              >  SRR4302545.6977891/1‑94 (MQ=60)
                                                                                 TTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCT             <  SRR4302545.473205/94‑1 (MQ=60)
                                                                                  TTGCGATTAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTC            <  SRR4302545.6079619/94‑1 (MQ=60)
                                                                                  TTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTC            <  SRR4302545.10656373/94‑1 (MQ=60)
                                                                                    GCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCC          >  SRR4302545.8399658/1‑94 (MQ=60)
                                                                                     CGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCT         <  SRR4302545.3132229/94‑1 (MQ=60)
                                                                                      GATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTC        <  SRR4302545.821156/94‑1 (MQ=60)
                                                                                      GATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTC        >  SRR4302545.6703200/1‑94 (MQ=60)
                                                                                      GATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTC        <  SRR4302545.1693935/94‑1 (MQ=60)
                                                                                       ATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCC       >  SRR4302545.1105101/1‑94 (MQ=60)
                                                                                        TGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGC      <  SRR4302545.8817678/94‑1 (MQ=60)
                                                                                         GAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATTGAAACTGATGCGCCGTATCTGCTCCCTCGCG     <  SRR4302545.7942314/94‑1 (MQ=60)
                                                                                         GAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCG     >  SRR4302545.7946546/1‑94 (MQ=60)
                                                                                         GAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCG     <  SRR4302545.5282008/94‑1 (MQ=60)
                                                                                           AACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGA    <  SRR4302545.4617585/94‑1 (MQ=60)
                                                                                           AACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGA    <  SRR4302545.2623382/94‑1 (MQ=60)
                                                                                            ACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGAT   <  SRR4302545.10323550/94‑1 (MQ=60)
                                                                                             |                                                                                             
TTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACCGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATC  >  NC_000913/4021591‑4021777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑