Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,021,684	Δ1 bp	coding (542/609 nt)\|coding (1/243 nt)	b4556 → tatD →	hypothetical protein Mg‑dependent DNase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,021,683	0	C	.	99.5%	993.9 / ‑3.6	213	coding (541/609 nt)	b4556	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (1/0); new base . (107/105); total (108/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 227 total aligned reads displayed.

TTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACCGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCG  >  NC_000913/4021591‑4021772

ttcttcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAAc                                                                                           >  1:57445/1‑93 (MQ=255)

ttcttcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAAc                                                                                           <  2:4903240/93‑1 (MQ=255)

 tcttcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAAcg                                                                                          >  2:5789394/1‑92 (MQ=255)

   ttcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAAcgac                                                                                        <  2:6017015/93‑4 (MQ=255)

    tcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAAcgacg                                                                                       >  2:4806229/1‑89 (MQ=255)

     cATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGAcgc                                                                                     >  2:6396458/1‑93 (MQ=255)

      aTTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGAcgcg                                                                                    >  2:1238133/1‑93 (MQ=255)

          cTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACt                                                                                >  2:4356101/1‑93 (MQ=255)

           tttACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTg                                                                               <  2:3048216/93‑1 (MQ=255)

            ttcccGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTgg                                                                              <  1:4943534/90‑1 (MQ=255)

            ttACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTgg                                                                              <  1:2852561/93‑1 (MQ=255)

            ttACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTgg                                                                              <  2:2355474/93‑1 (MQ=255)

                cGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCt                                                                          <  2:2466648/93‑1 (MQ=255)

                 ggCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTg                                                                         <  1:3058914/93‑1 (MQ=255)

                  gCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGc                                                                        >  1:2147260/1‑93 (MQ=255)

                   cacaCGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCg                                                                       >  1:1290747/1‑93 (MQ=255)

                    acacGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCgg                                                                      <  2:4192104/93‑1 (MQ=255)

                          gAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACtt                                                                >  1:4600529/1‑93 (MQ=255)

                          gAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACtt                                                                >  2:2830939/1‑93 (MQ=255)

                           aaGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACttt                                                               <  1:589533/93‑1 (MQ=255)

                            agagATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACtttt                                                              >  1:2162850/1‑93 (MQ=255)

                            agagATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACtttt                                                              <  2:937626/93‑1 (MQ=255)

                            agagATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACtttt                                                              >  1:4327638/1‑93 (MQ=255)

                              agaTGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGc                                                            <  1:1367171/93‑1 (MQ=255)

                               gaTGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGcc                                                           <  1:1999418/93‑1 (MQ=255)

                               gaTGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGcc                                                           >  1:5447095/1‑93 (MQ=255)

                               gaTGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGcc                                                           >  1:5973705/1‑93 (MQ=255)

                               gaTGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGcc                                                           >  2:2533921/1‑93 (MQ=255)

                                aTGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCg                                                          <  1:1880947/93‑1 (MQ=255)

                                 tGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGt                                                         >  1:4050551/1‑93 (MQ=255)

                                 tGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGt                                                         >  2:5678703/1‑93 (MQ=255)

                                 tGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTCTTGCCGt                                                         >  1:5129039/1‑93 (MQ=255)

                                  gCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGtt                                                        <  2:3755400/93‑1 (MQ=255)

                                  gCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGtt                                                        <  1:1192725/93‑1 (MQ=255)

                                   cAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTg                                                       <  1:5059153/93‑1 (MQ=255)

                                    aGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGa                                                      >  1:4656557/1‑93 (MQ=255)

                                    aGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGa                                                      >  2:5272284/1‑93 (MQ=255)

                                     ggCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGAt                                                     >  1:1066365/1‑93 (MQ=255)

                                     ggCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGAt                                                     >  2:1450640/1‑93 (MQ=255)

                                     ggCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGAt                                                     >  2:4058397/1‑93 (MQ=255)

                                     ggCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGAt                                                     >  2:5078735/1‑93 (MQ=255)

                                     ggCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGAt                                                     >  2:5089499/1‑93 (MQ=255)

                                       cgtgcgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTc                                                   <  1:1730178/93‑1 (MQ=255)

                                       cgtgcgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTc                                                   <  2:3011311/93‑1 (MQ=255)

                                       cgtgcgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTc                                                   <  2:2128922/93‑1 (MQ=255)

                                        gtgcgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTcc                                                  >  2:3665047/1‑93 (MQ=255)

                                        gtgcgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTcc                                                  <  1:3016917/93‑1 (MQ=255)

                                        gtgcgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTcc                                                  <  1:3599349/93‑1 (MQ=255)

                                         tgcgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCcg                                                 <  1:1794962/93‑1 (MQ=255)

                                         tgcgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCcg                                                 >  1:5675054/1‑93 (MQ=255)

                                         tgcgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCcg                                                 >  2:1245153/1‑93 (MQ=255)

                                           cgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCcggc                                               <  2:6117402/93‑1 (MQ=255)

                                            gtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCcggcg                                              >  1:5459122/1‑93 (MQ=255)

                                             tgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCcggcgg                                             >  1:5049224/1‑93 (MQ=255)

                                               gcgcATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGaa                                           <  1:4802023/93‑1 (MQ=255)

                                                cgcATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGaaa                                          >  1:1963732/1‑93 (MQ=255)

                                                   aTGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAAt                                       >  1:2267876/1‑93 (MQ=255)

                                                    tGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAAtt                                      <  2:926658/93‑1 (MQ=255)

                                                    tGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAAtt                                      >  1:3756603/1‑93 (MQ=255)

                                                    tGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAAtt                                      >  1:2719387/1‑93 (MQ=255)

                                                    tGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAAtt                                      <  1:1929927/93‑1 (MQ=255)

                                                    tGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAAtt                                      >  1:6157030/1‑93 (MQ=255)

                                                    tGGAATTTATATCGGAATTACCGGTTGGGTTTGGGATGAA‑GGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAAta                                      >  2:459802/1‑92 (MQ=255)

                                                      gAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTAc                                    <  2:661611/93‑1 (MQ=255)

                                                      gAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTAc                                    >  1:261515/1‑93 (MQ=255)

                                                         tttATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGa                                 >  2:4294500/1‑93 (MQ=255)

                                                         tttATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGa                                 >  2:3193471/1‑93 (MQ=255)

                                                          ttATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGAt                                <  1:6289271/93‑1 (MQ=255)

                                                          ttATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGAt                                <  1:5806743/93‑1 (MQ=255)

                                                           tataTCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATc                               <  2:1758887/93‑1 (MQ=255)

                                                           tataTCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATc                               <  1:5403798/93‑1 (MQ=255)

                                                           tataTCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATc                               <  1:890378/93‑1 (MQ=255)

                                                            atatCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCg                              <  1:3129500/93‑1 (MQ=255)

                                                             tatCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGa                             >  2:5732049/1‑93 (MQ=255)

                                                             tatCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGa                             >  1:4436936/1‑93 (MQ=255)

                                                               tCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGaaa                           <  2:4528793/93‑1 (MQ=255)

                                                                 ggCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACt                         <  1:4507878/93‑1 (MQ=255)

                                                                 ggCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACt                         <  1:6411401/93‑1 (MQ=255)

                                                                 ggCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATAGAAACt                         >  1:898207/1‑93 (MQ=255)

                                                                   cATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGa                       <  1:4110906/93‑1 (MQ=255)

                                                                    aTTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGAt                      <  2:1199346/93‑1 (MQ=255)

                                                                    aTTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGAt                      >  1:670510/1‑93 (MQ=255)

                                                                     ttACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATg                     >  1:3112543/1‑93 (MQ=255)

                                                                      tACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATgc                    <  1:55574/93‑1 (MQ=255)

                                                                      tACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATgc                    <  1:5004751/93‑1 (MQ=255)

                                                                      tACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATgc                    <  1:160182/93‑1 (MQ=255)

                                                                       aCCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAATTGATgcg                   >  1:5697153/1‑93 (MQ=255)

                                                                          ggTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCg                >  2:1346503/1‑93 (MQ=255)

                                                                           gTTGGGGTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGGACTTTTGCCGTTGATTCCGGCGGGAAAATTACTGATCGAAACTGATGCGCCGt               >  2:818283/1‑93 (MQ=255)

                                                                            ttaggTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTa              <  1:1440811/90‑1 (MQ=255)

                                                                            ttGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTa              >  1:5619068/1‑93 (MQ=255)

                                                                             tGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTAt             <  1:6300918/93‑1 (MQ=255)

                                                                              gggTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATc            <  2:2332801/93‑1 (MQ=255)

                                                                                gTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTg          <  2:1592933/93‑1 (MQ=255)

                                                                                 tttGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGc         <  2:1286204/93‑1 (MQ=255)

                                                                                 tttGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGc         <  2:3405812/93‑1 (MQ=255)

                                                                                      gATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCt    >  1:2513953/1‑93 (MQ=255)

                                                                                       aTGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTc   <  2:6084669/93‑1 (MQ=255)

                                                                                       aTGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTc   <  2:3255886/93‑1 (MQ=255)

                                                                                         tcaCCGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTcg  >  2:2720593/3‑93 (MQ=255)

TTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACCGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCG  >  NC_000913/4021591‑4021772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACCGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATC  >  NC_000913/4021594‑4021777

TTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACG                                                                                           >  SRR4302537.699267/1‑94 (MQ=60)

  CATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCG                                                                                         <  SRR4302537.6433545/94‑1 (MQ=60)

   ATTGCTGTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGG                                                                                        <  SRR4302537.576326/94‑1 (MQ=60)

    TTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGA                                                                                       <  SRR4302537.380443/94‑1 (MQ=60)

     TGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGAC                                                                                      <  SRR4302537.6052023/94‑1 (MQ=60)

     TGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGAC                                                                                      >  SRR4302537.2871558/1‑94 (MQ=60)

        TTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGG                                                                                   <  SRR4302537.5060669/94‑1 (MQ=60)

         TTCCCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGA                                                                                  <  SRR4302537.4977623/94‑1 (MQ=60)

         TTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGA                                                                                  <  SRR4302537.2872547/94‑1 (MQ=60)

            CCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCT                                                                               >  SRR4302537.119849/1‑94 (MQ=60)

            CCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCT                                                                               <  SRR4302537.5804442/94‑1 (MQ=60)

             CGGCACACGCGAAGAGATGCAGGGGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGAAGCGGACTGGAGCTG                                                                              >  SRR4302537.3069759/1‑94 (MQ=60)

              GGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGC                                                                             <  SRR4302537.3080227/94‑1 (MQ=60)

              GGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGC                                                                             >  SRR4302537.6300483/1‑94 (MQ=60)

              GGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGC                                                                             >  SRR4302537.2162363/1‑94 (MQ=60)

               GCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCG                                                                            >  SRR4302537.1299723/1‑94 (MQ=60)

                 ACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGG                                                                          <  SRR4302537.499196/94‑1 (MQ=60)

                 ACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGG                                                                          >  SRR4302537.2167331/1‑94 (MQ=60)

                   ACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAA                                                                        >  SRR4302537.4862980/1‑94 (MQ=60)

                    CGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAAC                                                                       >  SRR4302537.4663872/1‑94 (MQ=60)

                      CGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTT                                                                     >  SRR4302537.4632373/1‑94 (MQ=60)

                       GAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTT                                                                    >  SRR4302537.5093041/1‑94 (MQ=60)

                       GAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTT                                                                    <  SRR4302537.3213571/94‑1 (MQ=60)

                       GAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTT                                                                    <  SRR4302537.3691054/94‑1 (MQ=60)

                       GAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTT                                                                    >  SRR4302537.5577612/1‑94 (MQ=60)

                        AAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTT                                                                   <  SRR4302537.1600419/94‑1 (MQ=60)

                        AAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTT                                                                   >  SRR4302537.2178090/1‑94 (MQ=60)

                        AAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTT                                                                   >  SRR4302537.4357716/1‑94 (MQ=60)

                        AAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTT                                                                   <  SRR4302537.593558/94‑1 (MQ=60)

                        AAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTT                                                                   <  SRR4302537.4109780/94‑1 (MQ=60)

                          GAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGC                                                                 <  SRR4302537.6026089/94‑1 (MQ=60)

                           AGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCC                                                                <  SRR4302537.1376700/94‑1 (MQ=60)

                           AGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCC                                                                >  SRR4302537.6014711/1‑94 (MQ=60)

                           AGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCC                                                                >  SRR4302537.904095/1‑94 (MQ=60)

                           AGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCC                                                                >  SRR4302537.5484254/1‑94 (MQ=60)

                           AGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCC                                                                >  SRR4302537.5959134/1‑94 (MQ=60)

                           AGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCC                                                                <  SRR4302537.4085914/94‑1 (MQ=60)

                            GATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCG                                                               <  SRR4302537.2013321/94‑1 (MQ=60)

                             ATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGT                                                              >  SRR4302537.4078874/1‑94 (MQ=60)

                             ATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGT                                                              <  SRR4302537.1894028/94‑1 (MQ=60)

                             ATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTCTTGCCGT                                                              >  SRR4302537.5164383/1‑94 (MQ=60)

                               GCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTG                                                            <  SRR4302537.1201008/94‑1 (MQ=60)

                                CAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGA                                                           >  SRR4302537.4688748/1‑94 (MQ=60)

                                CAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGA                                                           <  SRR4302537.5093979/94‑1 (MQ=60)

                                 AGGCGTTCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGAT                                                          <  SRR4302537.1399769/94‑1 (MQ=60)

                                 AGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGAT                                                          >  SRR4302537.2335456/1‑94 (MQ=60)

                                 AGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGAT                                                          >  SRR4302537.1073710/1‑94 (MQ=60)

                                 AGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGAT                                                          <  SRR4302537.3483677/94‑1 (MQ=60)

                                    CGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCC                                                       >  SRR4302537.2209199/1‑94 (MQ=60)

                                    CGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCC                                                       <  SRR4302537.1742253/94‑1 (MQ=60)

                                     GTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCG                                                      >  SRR4302537.5713884/1‑94 (MQ=60)

                                     GTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCG                                                      <  SRR4302537.3624718/94‑1 (MQ=60)

                                     GTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCG                                                      >  SRR4302537.3106852/1‑94 (MQ=60)

                                     GTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCG                                                      <  SRR4302537.3037947/94‑1 (MQ=60)

                                      TGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGG                                                     <  SRR4302537.1807489/94‑1 (MQ=60)

                                      TGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGG                                                     <  SRR4302537.5638818/94‑1 (MQ=60)

                                       GCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGC                                                    <  SRR4302537.2506015/94‑1 (MQ=60)

                                        CGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCG                                                   >  SRR4302537.5496362/1‑94 (MQ=60)

                                         GTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGG                                                  >  SRR4302537.263408/1‑94 (MQ=60)

                                         GTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGG                                                  >  SRR4302537.5083989/1‑94 (MQ=60)

                                            GCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAA                                               <  SRR4302537.4835151/94‑1 (MQ=60)

                                            GCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAA                                               >  SRR4302537.1977386/1‑94 (MQ=60)

                                             CGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAA                                              >  SRR4302537.1719196/1‑94 (MQ=60)

                                               CATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAAT                                            >  SRR4302537.2283891/1‑94 (MQ=60)

                                               CATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAAT                                            <  SRR4302537.6510419/94‑1 (MQ=60)

                                                ATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATT                                           >  SRR4302537.2738380/1‑94 (MQ=60)

                                                ATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATT                                           >  SRR4302537.5239223/1‑94 (MQ=60)

                                                ATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATT                                           >  SRR4302537.3782976/1‑94 (MQ=60)

                                                ATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATT                                           >  SRR4302537.6199402/1‑94 (MQ=60)

                                                 TGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTA                                          <  SRR4302537.1943322/94‑1 (MQ=60)

                                                  GGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTAC                                         >  SRR4302537.5467681/1‑94 (MQ=60)

                                                  GGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTAC                                         >  SRR4302537.263359/1‑94 (MQ=60)

                                                    AATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTG                                       >  SRR4302537.5918421/1‑94 (MQ=60)

                                                    AATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTG                                       >  SRR4302537.1350382/1‑94 (MQ=60)

                                                    AATTTATATCGCCATTACTGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTG                                       <  SRR4302537.1414681/94‑1 (MQ=60)

                                                     ATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGA                                      >  SRR4302537.1933423/1‑94 (MQ=60)

                                                      TTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGAT                                     >  SRR4302537.2002522/1‑94 (MQ=60)

                                                       TTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATC                                    <  SRR4302537.5846562/94‑1 (MQ=60)

                                                       TTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATC                                    <  SRR4302537.5552027/94‑1 (MQ=60)

                                                       TTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATC                                    <  SRR4302537.6332380/94‑1 (MQ=60)

                                                       TTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATC                                    >  SRR4302537.1757193/1‑94 (MQ=60)

                                                        TATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGCGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCG                                   <  SRR4302537.215724/94‑1 (MQ=60)

                                                        TATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCG                                   <  SRR4302537.896508/94‑1 (MQ=60)

                                                        TATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCG                                   <  SRR4302537.5440668/94‑1 (MQ=60)

                                                         ATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGCACAATTACTGATCGA                                  >  SRR4302537.5699739/1‑94 (MQ=60)

                                                         ATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGA                                  >  SRR4302537.4467570/1‑94 (MQ=60)

                                                         ATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGA                                  <  SRR4302537.3151437/94‑1 (MQ=60)

                                                         ATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTAATTCCGGCGGAAAAATTACTGATCGA                                  >  SRR4302537.5484463/1‑94 (MQ=60)

                                                         ATATCGGCATTACCGGTTGGGCTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGA                                  >  SRR4302537.1831449/1‑94 (MQ=60)

                                                             CGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATAGAAACT                              >  SRR4302537.904400/1‑94 (MQ=60)

                                                              GGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTG                             <  SRR4302537.6455019/94‑1 (MQ=60)

                                                              GGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTG                             <  SRR4302537.6399123/94‑1 (MQ=60)

                                                              GGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTG                             <  SRR4302537.5726913/94‑1 (MQ=60)

                                                              GGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTG                             <  SRR4302537.4539051/94‑1 (MQ=60)

                                                                CATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGAT                           >  SRR4302537.675093/1‑94 (MQ=60)

                                                                CATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGAT                           <  SRR4302537.4426685/94‑1 (MQ=60)

                                                                CATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGAT                           <  SRR4302537.4139622/94‑1 (MQ=60)

                                                                 ATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATG                          >  SRR4302537.942034/1‑94 (MQ=60)

                                                                 ATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATG                          >  SRR4302537.1859395/1‑94 (MQ=60)

                                                                 ATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATG                          <  SRR4302537.5033629/94‑1 (MQ=60)

                                                                 ATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATG                          >  SRR4302537.3134283/1‑94 (MQ=60)

                                                                 ATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATG                          <  SRR4302537.5765160/94‑1 (MQ=60)

                                                                 ATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATG                          <  SRR4302537.4168178/94‑1 (MQ=60)

                                                                  TTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGC                         <  SRR4302537.4583681/94‑1 (MQ=60)

                                                                   TACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAATTGATGCG                        >  SRR4302537.5736142/1‑94 (MQ=60)

                                                                   TACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCG                        <  SRR4302537.55987/94‑1 (MQ=60)

                                                                   TACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCG                        <  SRR4302537.161319/94‑1 (MQ=60)

                                                                   TACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCG                        <  SRR4302537.5039220/94‑1 (MQ=60)

                                                                   TACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCG                        >  SRR4302537.4617230/1‑94 (MQ=60)

                                                                     CCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCC                      >  SRR4302537.5226381/1‑94 (MQ=60)

                                                                      CGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCG                     >  SRR4302537.1291414/1‑94 (MQ=60)

                                                                       GGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGT                    >  SRR4302537.5120783/1‑94 (MQ=60)

                                                                       GGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTCATCGAAACTGATGCGCCGT                    >  SRR4302537.2543891/1‑94 (MQ=60)

                                                                        GTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTA                   >  SRR4302537.5657492/1‑94 (MQ=60)

                                                                         TTAGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTAT                  <  SRR4302537.1450853/94‑1 (MQ=60)

                                                                          TGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATC                 <  SRR4302537.6344069/94‑1 (MQ=60)

                                                                           GGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAAGTACTGATCGAAACTGATGCGCCGTAGCT                >  SRR4302537.1950500/1‑94 (MQ=60)

                                                                            GGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTG               <  SRR4302537.6337465/94‑1 (MQ=60)

                                                                            GGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTG               <  SRR4302537.4817675/94‑1 (MQ=60)

                                                                             GTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGC              >  SRR4302537.4475351/1‑94 (MQ=60)

                                                                              TTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCT             <  SRR4302537.2100037/94‑1 (MQ=60)

                                                                               TTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTC            >  SRR4302537.2941898/1‑94 (MQ=60)

                                                                                TGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCC           <  SRR4302537.3141510/94‑1 (MQ=60)

                                                                                  CGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCT         >  SRR4302537.2531623/1‑94 (MQ=60)

                                                                                  CGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCT         >  SRR4302537.3027296/1‑94 (MQ=60)

                                                                                      GAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAGAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCG     >  SRR4302537.3729112/1‑94 (MQ=60)

                                                                                      GAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCG     <  SRR4302537.5334638/94‑1 (MQ=60)

                                                                                        AACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGA    <  SRR4302537.4509235/94‑1 (MQ=60)

                                                                                         ACGACGCGGACTGGAGCTGCGGGAACTTTTGCTGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGAT   <  SRR4302537.2308066/94‑1 (MQ=60)

                                                                                          CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATC  <  SRR4302537.1904086/94‑1 (MQ=60)

TTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACCGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATC  >  NC_000913/4021594‑4021777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`