Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,024,435 A→G I107V (ATT→GTT)  ubiB → ferrisiderophore reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,024,4350AG98.9% 1872.0 / 3.4 555I107V (ATT→GTT) ubiBferrisiderophore reductase
Reads supporting (aligned to +/- strand):  ref base A (2/4);  new base G (253/296);  total (255/300)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

Only 100 of 581 total aligned reads displayed.

AGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGATTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAACCGTGATATCACCATTT  >  NC_000913/4024344‑4024525
                                                                                           |                                                                                          
aGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGtt                                                                                          >  2:8279926/1‑94 (MQ=255)
  tCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGtttt                                                                                        <  2:10060335/94‑1 (MQ=255)
     tGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGAt                                                                                     <  2:5183416/94‑1 (MQ=255)
      ggACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGAtt                                                                                    <  1:2400470/94‑1 (MQ=255)
      ggACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGAtt                                                                                    <  2:4024318/94‑1 (MQ=255)
       gACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAATAGCGGCCGATGGTTTTGATTg                                                                                   >  2:1398501/1‑94 (MQ=255)
       gACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTg                                                                                   <  1:2515704/94‑1 (MQ=255)
        gccGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTgc                                                                                  <  2:3503870/93‑1 (MQ=255)
        aCCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTgc                                                                                  <  1:4855813/94‑1 (MQ=255)
         tcGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTgcg                                                                                 >  2:6321206/2‑94 (MQ=255)
          cGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGCGCAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGGTTTGACTgggg                                                                                >  1:5423349/1‑94 (MQ=255)
          cGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTgcgg                                                                                <  2:9232609/94‑1 (MQ=255)
            cATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTgcgggg                                                                              >  1:7679182/1‑93 (MQ=255)
            cATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTgcgggc                                                                              <  2:3332280/94‑1 (MQ=255)
             aTCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTgcgggcg                                                                             >  2:9894328/1‑94 (MQ=255)
              tCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTgcgggcgg                                                                            <  2:5266677/94‑1 (MQ=255)
              tCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTgcgggcgg                                                                            <  1:6084822/94‑1 (MQ=255)
                 tCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCAc                                                                         >  1:644112/1‑94 (MQ=255)
                  cAAAGATCATCAAATCGTGGTCGACATTCGACACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCAcc                                                                        <  2:10237235/94‑1 (MQ=255)
                  cAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGCGCGGCAcc                                                                        >  1:2025737/1‑94 (MQ=255)
                   aaaGATCATCAAATCGTGGTCGACATCCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCg                                                                       <  2:666503/94‑1 (MQ=255)
                    aaGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCgg                                                                      >  1:10423667/1‑94 (MQ=255)
                      aatcatcAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGt                                                                    <  1:7929607/93‑1 (MQ=255)
                       atcatcAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGtt                                                                   >  2:7641614/1‑94 (MQ=255)
                        tcatcaAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTtc                                                                  <  2:4972494/94‑1 (MQ=255)
                        tcatcaAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTtc                                                                  >  2:9797661/1‑94 (MQ=255)
                         catcaAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGTGGGCGGCCCCGGGTtct                                                                 >  1:1442550/1‑94 (MQ=255)
                            caAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCtt                                                              >  1:8172062/1‑94 (MQ=255)
                             aaaTCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTa                                                             <  2:10367226/94‑1 (MQ=255)
                             aaaTCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTCTGATGGCGGGCGGCACCGGGCTCTCTTa                                                             >  2:9408054/1‑94 (MQ=255)
                              aaTCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTAt                                                            >  2:8860283/1‑94 (MQ=255)
                               aTCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATg                                                           <  1:4100612/94‑1 (MQ=255)
                               aTCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATg                                                           <  1:3365380/94‑1 (MQ=255)
                                 cGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGcc                                                         >  1:6989989/1‑94 (MQ=255)
                                  gTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGccc                                                        <  2:3767486/94‑1 (MQ=255)
                                    ggTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGc                                                      <  2:2238972/94‑1 (MQ=255)
                                    ggTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGc                                                      <  1:8384224/94‑1 (MQ=255)
                                        gACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCgt                                                  >  1:1246768/1‑93 (MQ=255)
                                        gACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGa                                                  >  2:6922437/1‑94 (MQ=255)
                                        gACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGa                                                  <  2:1176043/94‑1 (MQ=255)
                                        gACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGa                                                  >  1:6555438/1‑94 (MQ=255)
                                          cATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGAtt                                                >  2:605083/1‑94 (MQ=255)
                                          cATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCAAtt                                                <  1:5237101/94‑1 (MQ=255)
                                           aTTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGAttt                                               >  2:7829836/1‑94 (MQ=255)
                                            ttCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGAtttt                                              <  1:8896823/94‑1 (MQ=255)
                                             tCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTg                                             <  2:9101772/94‑1 (MQ=255)
                                              ccccACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCTATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGc                                            >  1:5168292/1‑94 (MQ=255)
                                              ccccACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTgt                                            >  2:5634407/1‑93 (MQ=255)
                                               cccACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCt                                           >  2:416481/1‑94 (MQ=255)
                                               cccACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCt                                           <  1:9251146/94‑1 (MQ=255)
                                               cccACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCt                                           >  1:5895276/1‑94 (MQ=255)
                                                ccACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTg                                          <  1:7799539/94‑1 (MQ=255)
                                                 cACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGa                                         <  1:2173166/94‑1 (MQ=255)
                                                  acagagaAGCGTGGCTGCTCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTCCTGAc                                        >  1:10523188/4‑94 (MQ=255)
                                                   cGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACa                                       <  1:1006094/94‑1 (MQ=255)
                                                    ggAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAg                                      >  2:7347988/1‑94 (MQ=255)
                                                     gagaAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGc                                     <  2:7024195/94‑1 (MQ=255)
                                                      agaAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCg                                    >  1:4755661/1‑94 (MQ=255)
                                                        aaGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGtt                                  >  2:848019/1‑94 (MQ=255)
                                                        aaGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGtt                                  <  1:6845718/94‑1 (MQ=255)
                                                         aGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTg                                 <  2:235673/94‑1 (MQ=255)
                                                         aGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTg                                 <  2:4664526/94‑1 (MQ=255)
                                                         aGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTg                                 <  2:5942726/94‑1 (MQ=255)
                                                          gCGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTgg                                <  2:2889906/94‑1 (MQ=255)
                                                           cGTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGgc                               <  1:9665864/94‑1 (MQ=255)
                                                            gTGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGgcg                              <  2:3912358/94‑1 (MQ=255)
                                                             tGGCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGgcgc                             >  2:952709/1‑94 (MQ=255)
                                                              ggCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGcgcg                            <  1:2604665/94‑1 (MQ=255)
                                                               gCTGCGCGATGATGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGt                           <  1:8588578/94‑1 (MQ=255)
                                                                      gatgatGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCaa                    >  1:2783434/1‑94 (MQ=255)
                                                                      gatgatGAAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCaa                    <  2:8052027/94‑1 (MQ=255)
                                                                        tgatgaAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAAc                  >  1:3966598/1‑94 (MQ=255)
                                                                        tgatgaAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAAc                  <  2:3206949/94‑1 (MQ=255)
                                                                        tgatgaAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGCCGCGTAACCCAAAc                  <  1:6243315/94‑1 (MQ=255)
                                                                         gatgaAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAAcc                 >  1:369064/1‑94 (MQ=255)
                                                                         gatgaAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAAcc                 <  1:7493128/94‑1 (MQ=255)
                                                                          atgaAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAACCg                <  2:1959690/94‑1 (MQ=255)
                                                                           tgaAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGTGCGTAACCCAAACCGt               >  1:433589/1‑94 (MQ=255)
                                                                           tgaAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAACCGt               >  2:407492/1‑94 (MQ=255)
                                                                            gaAGAGCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAACCGTg              <  2:4347001/94‑1 (MQ=255)
                                                                              agagCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAACCGTGat            <  1:5593210/94‑1 (MQ=255)
                                                                              agagCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAACCGTGat            <  1:5742014/94‑1 (MQ=255)
                                                                               gagCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAACCGTGata           <  2:2531957/94‑1 (MQ=255)
                                                                               gagCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAACCGTGata           >  1:9430130/1‑94 (MQ=255)
                                                                               gagCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAACCGTGata           >  1:9885904/1‑94 (MQ=255)
                                                                                agCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAACCGTGatat          <  2:3663478/94‑1 (MQ=255)
                                                                                agCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAACCGTGatat          <  1:9033697/94‑1 (MQ=255)
                                                                                agCGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAACCGTGatat          <  2:10468027/94‑1 (MQ=255)
                                                                                  cGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAACCGTGATATCa        <  2:615837/94‑1 (MQ=255)
                                                                                  cGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAACCGTGATATCa        >  2:1787713/1‑94 (MQ=255)
                                                                                  cGTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAACCGTGATATCa        >  2:10593814/1‑94 (MQ=255)
                                                                                   gTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAACCGTGATATCAc       <  1:10297449/94‑1 (MQ=255)
                                                                                   gTCCGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCACTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAACCGTGATATCAc       >  2:5897923/1‑94 (MQ=255)
                                                                                     ccGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAACCGTGATATCACCa     <  1:7196294/94‑1 (MQ=255)
                                                                                      cGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAACCGTGATATCACCAt    >  1:3539050/1‑94 (MQ=255)
                                                                                      cGATGGTTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAACCGTGATATCACCAt    <  2:6467020/94‑1 (MQ=255)
                                                                                       gatggtTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAACCGTGATATCACCAtt   <  1:3017449/94‑1 (MQ=255)
                                                                                       gatggtTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAACCGTGATATCACCAtt   >  1:3776091/1‑94 (MQ=255)
                                                                                        atggtTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAACCGTGATATCACCAttt  <  2:2639786/94‑1 (MQ=255)
                                                                                        atggtTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAACCGTGATATCACCAttt  >  1:1721255/1‑94 (MQ=255)
                                                                                           |                                                                                          
AGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGATTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATTTTGCTGACAGCGTTGGCGCGTAACCCAAACCGTGATATCACCATTT  >  NC_000913/4024344‑4024525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 275 total aligned reads displayed.
GATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGCGTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATC  >  NC_000913/4026159‑4026341
                                                                                              |                                                                                         
GATATTAAACCCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGG                                                                                           <  SRR4302545.260154/94‑1 (MQ=60)
GATATTAAAACCCTGCTCCAGTGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGG                                                                                           >  SRR4302545.633704/1‑94 (MQ=60)
GATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGG                                                                                           >  SRR4302545.9837481/1‑94 (MQ=60)
GATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGG                                                                                           >  SRR4302545.4979099/1‑94 (MQ=60)
GATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGG                                                                                           <  SRR4302545.8979332/94‑1 (MQ=60)
GATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGGTACGCGCCAG‑CAGG                                                                                           >  SRR4302545.1392096/1‑94 (MQ=60)
     TAAACCCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACG                                                                                      <  SRR4302545.6460671/94‑1 (MQ=60)
     TAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGACGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACG                                                                                      <  SRR4302545.6785660/94‑1 (MQ=60)
      AAACCCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGC                                                                                     <  SRR4302545.2000684/94‑1 (MQ=60)
      AAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGC                                                                                     >  SRR4302545.3356685/1‑94 (MQ=60)
       AAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCA                                                                                    >  SRR4302545.10738535/1‑94 (MQ=60)
       AAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCA                                                                                    >  SRR4302545.3796036/1‑94 (MQ=60)
       AAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGGTACGCGCCAG‑CAGGCTACGCA                                                                                    >  SRR4302545.7457136/1‑94 (MQ=60)
         ACCCTGCTCCAGCGTCTTCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCACTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATT                                                                                  >  SRR4302545.7973461/1‑94 (MQ=60)
         ACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCAGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATT                                                                                  >  SRR4302545.7238122/1‑94 (MQ=60)
         ACCCTGCTCCAGCGCCGGCTGCACACACCCCCAGTAAATAGCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATT                                                                                  <  SRR4302545.174445/94‑1 (MQ=60)
          CCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTA                                                                                 <  SRR4302545.819545/94‑1 (MQ=60)
           CCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGGTACGCGCCAG‑CAGGCTACGCATTAA                                                                                <  SRR4302545.10451746/94‑1 (MQ=60)
            CTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAA                                                                               >  SRR4302545.5177198/1‑94 (MQ=60)
            CTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAA                                                                               >  SRR4302545.509535/1‑94 (MQ=60)
             TGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAAT                                                                              <  SRR4302545.377511/94‑1 (MQ=60)
             TGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAAT                                                                              >  SRR4302545.4954677/1‑94 (MQ=60)
             CGCTCCAGCTTCTGCTTCCCACACCCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAAT                                                                              <  SRR4302545.8447026/94‑1 (MQ=60)
              GCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATG                                                                             <  SRR4302545.10699178/94‑1 (MQ=60)
               CTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGA                                                                            >  SRR4302545.218175/1‑94 (MQ=60)
               CTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGA                                                                            >  SRR4302545.9399007/1‑94 (MQ=60)
                TCCAGCGTCTGCTGCACACAGCCCCAGTAAATATCGGCGAGGCCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAG                                                                           <  SRR4302545.1264494/94‑1 (MQ=60)
                TCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAG                                                                           <  SRR4302545.10726600/94‑1 (MQ=60)
                TCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAG                                                                           >  SRR4302545.3684257/1‑94 (MQ=60)
                TCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCATCCAGCGCCGGGGTACGTGCCAG‑CAGGCTACGCATTAAATGAG                                                                           >  SRR4302545.7035881/1‑94 (MQ=60)
                CCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAG                                                                           <  SRR4302545.7718863/94‑1 (MQ=60)
                 CCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGC                                                                          >  SRR4302545.9834198/1‑94 (MQ=60)
                 CCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGC                                                                          >  SRR4302545.10663138/1‑94 (MQ=60)
                 CCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGC                                                                          >  SRR4302545.6392600/1‑94 (MQ=60)
                  CAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCG                                                                         >  SRR4302545.6624324/1‑94 (MQ=60)
                   AGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGG                                                                        >  SRR4302545.9919195/1‑94 (MQ=60)
                    GCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCCTTAAATGAGCGGA                                                                       >  SRR4302545.165819/1‑94 (MQ=60)
                    GCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGA                                                                       <  SRR4302545.9721722/94‑1 (MQ=60)
                     CGTCTGCTGCCCACAACCCCAGTAAATACCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAG                                                                      <  SRR4302545.7433599/94‑1 (MQ=60)
                     CGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAG                                                                      >  SRR4302545.1744712/1‑94 (MQ=60)
                      GTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCCGCGCCGGGGTACGCGCCAGCCAGC‑CACGCATTAAAAGAGCGGAGA                                                                     >  SRR4302545.6186981/1‑94 (MQ=60)
                      GTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGG                                                                     >  SRR4302545.3120116/1‑94 (MQ=60)
                      GTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGA                                                                     >  SRR4302545.2131363/1‑94 (MQ=60)
                      GTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGA                                                                     >  SRR4302545.3007331/1‑94 (MQ=60)
                      GTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGA                                                                     <  SRR4302545.4518628/94‑1 (MQ=60)
                      GTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGA                                                                     >  SRR4302545.5970786/1‑94 (MQ=60)
                      GTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGCTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGA                                                                     >  SRR4302545.1543072/1‑94 (MQ=60)
                        CTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGA                                                                   >  SRR4302545.7217285/1‑94 (MQ=60)
                        CTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGA                                                                   >  SRR4302545.4076711/1‑94 (MQ=60)
                         TGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGAT                                                                  <  SRR4302545.1609896/94‑1 (MQ=60)
                         TGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGAT                                                                  <  SRR4302545.5114573/94‑1 (MQ=60)
                          GCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATC                                                                 >  SRR4302545.2904074/1‑94 (MQ=60)
                          GCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATC                                                                 <  SRR4302545.10795223/94‑1 (MQ=60)
                          GCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATC                                                                 <  SRR4302545.10337942/94‑1 (MQ=60)
                           CTGCACACACCCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCT                                                                <  SRR4302545.7883688/94‑1 (MQ=60)
                           CTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCT                                                                <  SRR4302545.6448183/94‑1 (MQ=60)
                           CTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGGTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCC                                                                >  SRR4302545.3199098/1‑94 (MQ=60)
                           CTGCACACAACCCCAGTAAATATCGTCGAGGGCCGACGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCT                                                                >  SRR4302545.50819/1‑94 (MQ=60)
                            TGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTT                                                               >  SRR4302545.6985964/1‑94 (MQ=60)
                            TGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGATACGCGCCAG‑CAGGCTACGCACTAAATGAACAGAGAAATCTT                                                               >  SRR4302545.9977570/1‑94 (MQ=60)
                              CACACACCCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCT                                                             <  SRR4302545.7649718/94‑1 (MQ=60)
                              CACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCT                                                             >  SRR4302545.7455279/1‑94 (MQ=60)
                               CCACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTG                                                            <  SRR4302545.1439646/94‑1 (MQ=60)
                               ACACACCCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTG                                                            <  SRR4302545.507875/94‑1 (MQ=60)
                               ACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTG                                                            >  SRR4302545.8302589/1‑94 (MQ=60)
                               ACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTG                                                            <  SRR4302545.8136136/94‑1 (MQ=60)
                               ACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTG                                                            <  SRR4302545.10378515/94‑1 (MQ=60)
                               ACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTG                                                            >  SRR4302545.10437956/1‑94 (MQ=60)
                                ccccaccccagaaaaaggtcgccggggccccccccTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGC                                                           <  SRR4302545.348947/59‑1 (MQ=60)
                                 ACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCA                                                          <  SRR4302545.2703613/94‑1 (MQ=60)
                                  CACCCCCAGTAAATATCCTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCAC                                                         <  SRR4302545.10396218/94‑1 (MQ=60)
                                  CAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCAC                                                         >  SRR4302545.184162/1‑94 (MQ=60)
                                  CAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCAC                                                         >  SRR4302545.462513/1‑94 (MQ=60)
                                   AACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACG                                                        <  SRR4302545.10318575/94‑1 (MQ=60)
                                    ACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGC                                                       >  SRR4302545.8119732/1‑94 (MQ=60)
                                     CCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCA                                                      >  SRR4302545.5704517/1‑94 (MQ=60)
                                     CCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCA                                                      >  SRR4302545.4376464/1‑94 (MQ=60)
                                     CCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCA                                                      >  SRR4302545.9241493/1‑94 (MQ=60)
                                      CCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGGTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCAC                                                     >  SRR4302545.889229/1‑94 (MQ=60)
                                       CCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACG                                                    >  SRR4302545.7736449/1‑94 (MQ=60)
                                        CAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGT                                                   <  SRR4302545.6892035/94‑1 (MQ=60)
                                        CAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGT                                                   >  SRR4302545.4737784/1‑94 (MQ=60)
                                        CAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGT                                                   >  SRR4302545.1862896/1‑94 (MQ=60)
                                        CAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGT                                                   <  SRR4302545.7557803/94‑1 (MQ=60)
                                         AGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTT                                                  >  SRR4302545.921850/1‑94 (MQ=60)
                                         AGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTT                                                  <  SRR4302545.9794405/94‑1 (MQ=60)
                                          GTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTA                                                 >  SRR4302545.8045335/1‑94 (MQ=60)
                                          GTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTA                                                 <  SRR4302545.8087332/94‑1 (MQ=60)
                                           TAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTAC                                                <  SRR4302545.2026067/94‑1 (MQ=60)
                                           TAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTAC                                                <  SRR4302545.10604451/94‑1 (MQ=60)
                                           TAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTAC                                                <  SRR4302545.5653751/94‑1 (MQ=60)
                                           TAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTAC                                                >  SRR4302545.34066/1‑94 (MQ=60)
                                           TAAATATCGTCGAGGCCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTAC                                                <  SRR4302545.78243/94‑1 (MQ=60)
                                            AAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACG                                               >  SRR4302545.3555047/1‑94 (MQ=60)
                                            AAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACG                                               <  SRR4302545.2185021/94‑1 (MQ=60)
                                            AAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACG                                               >  SRR4302545.10698091/1‑94 (MQ=60)
                                            AAATATCGTAGAGGGCAGCCGCTTGCAGCGCCGGGGTAGGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGATACG                                               >  SRR4302545.7332829/1‑94 (MQ=60)
                                               TATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAA                                            <  SRR4302545.9568551/94‑1 (MQ=60)
                                               TATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAA                                            <  SRR4302545.1763004/94‑1 (MQ=60)
                                                ATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAA                                           <  SRR4302545.2791439/94‑1 (MQ=60)
                                                ATCGTCGAGGGCCGCCGCTCCCGGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAA                                           >  SRR4302545.9230286/1‑94 (MQ=60)
                                                 TCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAG                                          <  SRR4302545.2931371/94‑1 (MQ=60)
                                                 TCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAG                                          <  SRR4302545.545109/94‑1 (MQ=60)
                                                 TCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAG                                          <  SRR4302545.5919849/94‑1 (MQ=60)
                                                  CGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGC                                         <  SRR4302545.7986071/94‑1 (MQ=60)
                                                  CGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGC                                         <  SRR4302545.1461004/94‑1 (MQ=60)
                                                   GTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCA                                        <  SRR4302545.9462311/94‑1 (MQ=60)
                                                   GTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCA                                        >  SRR4302545.1580563/1‑94 (MQ=60)
                                                   GTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCA                                        >  SRR4302545.5658489/1‑94 (MQ=60)
                                                    TCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCAC                                       >  SRR4302545.8490614/1‑94 (MQ=60)
                                                    TCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCAC                                       <  SRR4302545.704980/94‑1 (MQ=60)
                                                    GCGAGGCCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCAC                                       <  SRR4302545.3955365/94‑1 (MQ=60)
                                                     CGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACC                                      <  SRR4302545.3682354/94‑1 (MQ=60)
                                                     CGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACC                                      >  SRR4302545.10723735/1‑94 (MQ=60)
                                                     CGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACC                                      <  SRR4302545.8557450/94‑1 (MQ=60)
                                                     CGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACC                                      <  SRR4302545.782028/94‑1 (MQ=60)
                                                      GAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTATGAAAAGCACCG                                     >  SRR4302545.5988193/1‑94 (MQ=60)
                                                      GAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCG                                     <  SRR4302545.5068202/94‑1 (MQ=60)
                                                      GAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCG                                     >  SRR4302545.6572387/1‑94 (MQ=60)
                                                       TGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGC                                    >  SRR4302545.1404098/1‑94 (MQ=60)
                                                       AGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGC                                    <  SRR4302545.7269392/94‑1 (MQ=60)
                                                        GGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCC                                   <  SRR4302545.7727189/94‑1 (MQ=60)
                                                        GGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCC                                   <  SRR4302545.4230028/94‑1 (MQ=60)
                                                        GGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCC                                   <  SRR4302545.326753/94‑1 (MQ=60)
                                                        GGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCC                                   >  SRR4302545.8699432/1‑94 (MQ=60)
                                                         GGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCC                                  <  SRR4302545.9105322/94‑1 (MQ=60)
                                                         GGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCC                                  <  SRR4302545.4651494/94‑1 (MQ=60)
                                                         GGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCC                                  <  SRR4302545.5675428/94‑1 (MQ=60)
                                                         GGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCC                                  <  SRR4302545.3808419/94‑1 (MQ=60)
                                                          GCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCT                                 <  SRR4302545.3735132/94‑1 (MQ=60)
                                                            CGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTC                               >  SRR4302545.246623/1‑94 (MQ=60)
                                                            CGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTC                               >  SRR4302545.238066/1‑94 (MQ=60)
                                                            CGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTC                               >  SRR4302545.9400869/1‑94 (MQ=60)
                                                            CGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTC                               >  SRR4302545.7978519/1‑94 (MQ=60)
                                                             GCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCG                              <  SRR4302545.3579784/94‑1 (MQ=60)
                                                             GCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCG                              >  SRR4302545.8845315/1‑94 (MQ=60)
                                                             GCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCG                              <  SRR4302545.8359125/94‑1 (MQ=60)
                                                             CCCGCTTCCAGCGCCGGGTTACGAGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCG                              <  SRR4302545.6006779/94‑1 (MQ=60)
                                                               CGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAC                            >  SRR4302545.10903534/1‑94 (MQ=60)
                                                               CGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAA                            >  SRR4302545.3481110/1‑94 (MQ=60)
                                                               CGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAA                            >  SRR4302545.4874201/1‑94 (MQ=60)
                                                               CGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAAGGAGCGGAGATATCTTCTGTACGGGTGTTACGAAAAGCACCGCCCTGCGAA                            <  SRR4302545.1277452/94‑1 (MQ=53)
                                                                GCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAAC                           <  SRR4302545.7778217/94‑1 (MQ=60)
                                                                GCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAAC                           >  SRR4302545.6318785/1‑94 (MQ=60)
                                                                GCTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAAC                           >  SRR4302545.4734057/1‑94 (MQ=60)
                                                                 CTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACG                          <  SRR4302545.9139871/94‑1 (MQ=60)
                                                                 CTTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACG                          <  SRR4302545.6750220/94‑1 (MQ=60)
                                                                  TTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGG                         <  SRR4302545.2874497/94‑1 (MQ=60)
                                                                  TTCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGG                         <  SRR4302545.6129198/94‑1 (MQ=60)
                                                                   TCCAGCGCCGTGTTACGCGCCAG‑CAGGCTACTCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGC                        <  SRR4302545.788372/94‑1 (MQ=60)
                                                                   TCCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGC                        <  SRR4302545.8202943/94‑1 (MQ=60)
                                                                   TCCAGCGCCGGATTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGC                        <  SRR4302545.10099104/94‑1 (MQ=60)
                                                                    CCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCC                       <  SRR4302545.4566543/94‑1 (MQ=60)
                                                                    CCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCC                       <  SRR4302545.8847079/94‑1 (MQ=60)
                                                                    CCAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCC                       >  SRR4302545.5349885/1‑94 (MQ=60)
                                                                     CAGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCC                      >  SRR4302545.8477490/1‑94 (MQ=60)
                                                                      AGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCA                     <  SRR4302545.3594400/94‑1 (MQ=60)
                                                                      AGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCA                     <  SRR4302545.380727/94‑1 (MQ=60)
                                                                      AGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCA                     >  SRR4302545.4251188/1‑94 (MQ=60)
                                                                      AGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCA                     <  SRR4302545.8929523/94‑1 (MQ=60)
                                                                      AGCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCA                     >  SRR4302545.2014124/1‑94 (MQ=60)
                                                                       GCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCAT                    >  SRR4302545.7778193/1‑94 (MQ=60)
                                                                       GCGCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCAT                    <  SRR4302545.2334572/94‑1 (MQ=60)
                                                                         GCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCG                  >  SRR4302545.8332464/1‑94 (MQ=60)
                                                                         GCCGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCG                  >  SRR4302545.2838302/1‑94 (MQ=60)
                                                                           CGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGG                >  SRR4302545.3250213/1‑94 (MQ=60)
                                                                           CGGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGG                <  SRR4302545.3962193/94‑1 (MQ=60)
                                                                           CGGGTCACGCGCCAG‑GAGGCTACGCATTAAAGGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGG                <  SRR4302545.7791445/94‑1 (MQ=60)
                                                                            GGGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGG               >  SRR4302545.3232624/1‑94 (MQ=60)
                                                                             GGTTACGCGGCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAGAGCACCGCCCTTCGAACGGCCCATCGGGGT              <  SRR4302545.10844972/94‑1 (MQ=60)
                                                                             GGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGT              <  SRR4302545.138924/94‑1 (MQ=60)
                                                                             GGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGT              <  SRR4302545.10015091/94‑1 (MQ=60)
                                                                             GGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGG              >  SRR4302545.10161279/1‑94 (MQ=60)
                                                                             GGTTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTATGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGT              >  SRR4302545.1638269/1‑94 (MQ=60)
                                                                               TTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGC            <  SRR4302545.8870851/94‑1 (MQ=60)
                                                                               TTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGC            <  SRR4302545.4491288/94‑1 (MQ=60)
                                                                               TTACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGC            >  SRR4302545.10923502/1‑94 (MQ=60)
                                                                               TGACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGATATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGC            <  SRR4302545.4502834/94‑1 (MQ=60)
                                                                                TACGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCG           <  SRR4302545.1470642/94‑1 (MQ=60)
                                                                                  CGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAA         >  SRR4302545.6491163/1‑94 (MQ=60)
                                                                                  CGCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAA         <  SRR4302545.5767350/94‑1 (MQ=60)
                                                                                   GCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAAT        <  SRR4302545.6505800/94‑1 (MQ=60)
                                                                                   GCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAAT        <  SRR4302545.4536030/94‑1 (MQ=60)
                                                                                   GCGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAAT        <  SRR4302545.6321100/94‑1 (MQ=60)
                                                                                    CGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATT       <  SRR4302545.6968214/94‑1 (MQ=60)
                                                                                    CGCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGCGCGAATT       >  SRR4302545.107890/1‑94 (MQ=60)
                                                                                     GCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTG      <  SRR4302545.5313373/94‑1 (MQ=60)
                                                                                     GCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTG      <  SRR4302545.8051682/94‑1 (MQ=60)
                                                                                     GCCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTG      <  SRR4302545.7480209/94‑1 (MQ=60)
                                                                                      CCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGC     <  SRR4302545.8703914/94‑1 (MQ=60)
                                                                                      CCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGC     >  SRR4302545.2973115/1‑94 (MQ=60)
                                                                                      CCAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGC     >  SRR4302545.744578/1‑94 (MQ=60)
                                                                                       CAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCA    >  SRR4302545.407634/1‑94 (MQ=60)
                                                                                       CAG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCA    <  SRR4302545.471235/94‑1 (MQ=60)
                                                                                        AG‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCAT   <  SRR4302545.10604163/94‑1 (MQ=60)
                                                                                         G‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATC  >  SRR4302545.4016420/1‑94 (MQ=60)
                                                                                         G‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATC  <  SRR4302545.6993833/94‑1 (MQ=60)
                                                                                         G‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATC  >  SRR4302545.3814469/1‑94 (MQ=60)
                                                                                         G‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATC  >  SRR4302545.6597660/1‑94 (MQ=60)
                                                                                         G‑CAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATC  <  SRR4302545.5592856/94‑1 (MQ=60)
                                                                                              |                                                                                         
GATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAG‑CAGCGTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCATC  >  NC_000913/4026159‑4026341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑