Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,026,252 2 bp→GC coding (110‑111/1164 nt) fadA ← thiolase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,026,2520CG100.0% 1717.2 / NA 501T37S (ACG→AGC) fadAthiolase I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (232/269);  total (232/269)
*NC_0009134,026,2530GC99.8% 1714.5 / ‑5.2 504T37S (ACG→AGC) fadAthiolase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (232/271);  minor base A (0/1);  total (232/272)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

Only 100 of 518 total aligned reads displayed.

ATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGCGTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCAT  >  NC_000913/4026160‑4026340
                                                                                            ||                                                                                       
    tAAACCCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACg                                                                                     <  1:6289183/94‑1 (MQ=255)
    tAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACg                                                                                     >  2:8584519/1‑94 (MQ=255)
      aaaCCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCa                                                                                   >  1:3705157/1‑94 (MQ=255)
       aaCCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCAt                                                                                  >  2:1172436/1‑94 (MQ=255)
          ccTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTaa                                                                               >  2:2974276/1‑94 (MQ=255)
          ccTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCCGGCTACGCGCCAGCAGGCTACGCATTaa                                                                               >  2:1879835/1‑94 (MQ=255)
           cTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTaaa                                                                              >  1:5048857/1‑94 (MQ=255)
           cTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTaaa                                                                              >  2:8381253/1‑94 (MQ=255)
            tGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAAt                                                                             <  2:970387/94‑1 (MQ=255)
            cgCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAAt                                                                             <  1:3186702/93‑1 (MQ=255)
              cTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGa                                                                           >  2:4378941/1‑94 (MQ=255)
              cTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGa                                                                           >  1:213735/1‑94 (MQ=255)
               cccAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAg                                                                          <  1:7506472/93‑1 (MQ=255)
                ccAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCCGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAgg                                                                         >  2:2459731/1‑93 (MQ=255)
                ccAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGc                                                                         >  1:72801/1‑94 (MQ=255)
                ccAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGc                                                                         <  2:1527170/94‑1 (MQ=255)
                ccAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGc                                                                         >  2:7797435/1‑94 (MQ=255)
                 cAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCg                                                                        >  1:6448026/1‑94 (MQ=255)
                 cAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCg                                                                        <  2:4704647/94‑1 (MQ=255)
                  aGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCgg                                                                       >  1:10302765/1‑94 (MQ=255)
                  aGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCgg                                                                       >  2:590159/1‑94 (MQ=255)
                    ggTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGgag                                                                     <  2:8115623/93‑1 (MQ=255)
                    cGTCTGCTGCCCACAACCCCAGTAAATACCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGgag                                                                     <  1:7231500/94‑1 (MQ=255)
                    cGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGgag                                                                     >  2:3826915/1‑94 (MQ=255)
                     gTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGgagg                                                                    >  1:3045476/1‑93 (MQ=255)
                      tCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGgagag                                                                   <  1:6728148/94‑1 (MQ=255)
                      tCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGgagag                                                                   >  2:8916713/1‑94 (MQ=255)
                        tgctgcACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAt                                                                 <  2:6551707/94‑1 (MQ=255)
                        tgctgcACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAt                                                                 >  2:4787498/1‑94 (MQ=255)
                         gctgcACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAtc                                                                >  1:2835483/1‑94 (MQ=255)
                         gctgcACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAtc                                                                <  2:3645405/94‑1 (MQ=255)
                             cacacaACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGAtcttct                                                            >  2:917275/1‑94 (MQ=255)
                              ccacaACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTg                                                           <  1:1407870/93‑1 (MQ=255)
                              acacacCCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTg                                                           <  1:496757/94‑1 (MQ=255)
                              acacaACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTg                                                           >  1:8071313/1‑94 (MQ=255)
                              acacaACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTg                                                           >  1:10138140/1‑94 (MQ=255)
                                acaACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTgca                                                         >  2:6304041/1‑94 (MQ=255)
                                 caACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTgcac                                                        >  1:452484/1‑94 (MQ=255)
                                 caACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTgcac                                                        >  1:180318/1‑94 (MQ=255)
                                  aaCCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacg                                                       <  1:10021562/94‑1 (MQ=255)
                                   aCCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacgc                                                      >  1:7895635/1‑94 (MQ=255)
                                    ccccAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTgcacgca                                                     >  1:5557529/1‑94 (MQ=255)
                                        aGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGtt                                                 <  1:9470415/94‑1 (MQ=255)
                                         gTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTa                                                <  2:7294888/94‑1 (MQ=255)
                                          tAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTAc                                               <  1:5508492/94‑1 (MQ=255)
                                          tAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTAc                                               <  1:1977099/94‑1 (MQ=255)
                                          tAAATATCGTCGAGGCCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTAc                                               <  1:76526/94‑1 (MQ=255)
                                           aaaTATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACg                                              <  1:2716748/94‑1 (MQ=255)
                                            aaTATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGa                                             <  2:5471266/94‑1 (MQ=255)
                                             atatCGGCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGaa                                            <  2:6615873/94‑1 (MQ=255)
                                               atCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGaaaa                                          <  2:1467976/94‑1 (MQ=255)
                                               atCGTCGAGGGCCGCCGCTCCCGGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGaaaa                                          >  1:8969400/1‑94 (MQ=255)
                                                tcgtcgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAg                                         <  2:2323490/94‑1 (MQ=255)
                                                 cgtcgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGc                                        >  2:3159237/1‑94 (MQ=255)
                                                 cgtcgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGc                                        <  1:7766264/94‑1 (MQ=255)
                                                  gtcgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCa                                       >  1:1544313/1‑94 (MQ=255)
                                                   tcgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCAc                                      <  1:689539/94‑1 (MQ=255)
                                                    cgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCAcc                                     >  1:10416744/1‑94 (MQ=255)
                                                    cgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCAcc                                     <  1:3594662/94‑1 (MQ=255)
                                                    cgAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACCAAAAGCAcc                                     >  2:7919028/1‑94 (MQ=255)
                                                     gAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCAcgc                                    >  2:2249547/1‑92 (MQ=255)
                                                     gAGGCCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCg                                    <  2:2165410/94‑1 (MQ=255)
                                                      aGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGc                                   <  2:450358/94‑1 (MQ=255)
                                                       gggCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGcc                                  >  1:8455053/1‑94 (MQ=255)
                                                       gggCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGcc                                  >  1:1631729/1‑94 (MQ=255)
                                                        ggCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGccc                                 >  2:10057931/1‑94 (MQ=255)
                                                           cgccgcTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTc                              >  1:9132672/1‑94 (MQ=255)
                                                           cgccgcTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTc                              >  2:5206916/1‑94 (MQ=255)
                                                           cgccgcTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTc                              <  2:8023223/94‑1 (MQ=255)
                                                            cccgcTTCCAGCGCCGGGTTACGAGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCg                             <  1:5849260/93‑1 (MQ=255)
                                                              cgcTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGaa                           >  1:4752808/1‑94 (MQ=255)
                                                              cgcTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGaa                           >  1:3398267/1‑94 (MQ=255)
                                                               gcTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAAc                          <  1:7564411/94‑1 (MQ=255)
                                                               gcTTCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAAc                          >  1:1127237/1‑94 (MQ=255)
                                                                 ttCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACgg                        >  2:7706860/1‑94 (MQ=255)
                                                                 ttCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACgg                        >  2:9900582/1‑94 (MQ=255)
                                                                  tCCAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGc                       <  2:6995357/94‑1 (MQ=255)
                                                                    cAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGccc                     <  2:9010487/94‑1 (MQ=255)
                                                                    cAGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGccc                     >  1:8238966/1‑94 (MQ=255)
                                                                     aGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCa                    <  2:9338287/94‑1 (MQ=255)
                                                                     aGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCa                    >  1:4147592/1‑94 (MQ=255)
                                                                     aGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCa                    <  2:3978276/94‑1 (MQ=255)
                                                                     aGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCa                    >  2:3442985/1‑94 (MQ=255)
                                                                     aGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCa                    <  2:10575406/94‑1 (MQ=255)
                                                                     aGCGCCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCa                    <  2:10247968/94‑1 (MQ=255)
                                                                      gcgcCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCAt                   <  2:6176266/94‑1 (MQ=255)
                                                                        gcCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCg                 <  2:5678542/94‑1 (MQ=255)
                                                                        gcCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCg                 <  2:7451117/94‑1 (MQ=255)
                                                                        gcCGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCg                 <  2:5395840/94‑1 (MQ=255)
                                                                         ccGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCGCGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCgg                >  2:2657927/1‑94 (MQ=255)
                                                                         ccGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCgg                >  2:10344036/1‑94 (MQ=255)
                                                                         ccGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCgg                >  2:6053295/1‑94 (MQ=255)
                                                                          cGGGTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCggg               <  1:3865694/94‑1 (MQ=255)
                                                                            ggTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGt             <  2:3368530/94‑1 (MQ=255)
                                                                            ggTTACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGt             <  1:10534634/94‑1 (MQ=255)
                                                                              ttACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGc           <  1:8621153/94‑1 (MQ=255)
                                                                              ttACGCGCCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGc           <  1:4391566/94‑1 (MQ=255)
                                                                                  gcgcCAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAAt       <  1:6153648/94‑1 (MQ=255)
                                                                                     ccAGCAGGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGc    >  1:9704474/1‑94 (MQ=255)
                                                                                      cagcagGCTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCa   <  2:4144896/94‑1 (MQ=255)
                                                                                            ||                                                                                       
ATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCCGCTTCCAGCGCCGGGTTACGCGCCAGCAGCGTACGCATTAAATGAGCGGAGAGATCTTCTGCACGCACGTTACGAAAAGCACCGCCCTTCGAACGGCCCATCGGGGTGCGAATTGCAT  >  NC_000913/4026160‑4026340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 287 total aligned reads displayed.
TGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCTAAC  >  NC_000913/4030281‑4030467
                                                                                             |                                                                                             
TGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGC                                                                                               <  SRR4302545.8775972/94‑1 (MQ=60)
 GGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCG                                                                                              <  SRR4302545.5447434/94‑1 (MQ=60)
 GGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCG                                                                                              <  SRR4302545.9820550/94‑1 (MQ=60)
  GGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGG                                                                                             <  SRR4302545.2386518/94‑1 (MQ=60)
   gttctTCTGACGACGGGGAGCCGGCGGAAACGGCAGGTAACCCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGT                                                                                            <  SRR4302545.5295182/89‑1 (MQ=60)
   GTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGT                                                                                            <  SRR4302545.6731983/94‑1 (MQ=60)
   GTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGT                                                                                            <  SRR4302545.9225536/94‑1 (MQ=60)
   GTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGT                                                                                            <  SRR4302545.3238010/94‑1 (MQ=60)
   GTTTCTCGGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGT                                                                                            <  SRR4302545.10259124/94‑1 (MQ=60)
    TTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTA                                                                                           <  SRR4302545.3026811/94‑1 (MQ=60)
    TTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTA                                                                                           <  SRR4302545.7642391/94‑1 (MQ=60)
     TTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAG                                                                                          >  SRR4302545.59907/1‑94 (MQ=60)
      TCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGT                                                                                         >  SRR4302545.1210812/1‑94 (MQ=60)
       CTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGGAATTACCGCTGCGGGAGTG                                                                                        >  SRR4302545.9812340/1‑94 (MQ=60)
       CTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGCAATTACCGCTGCCGTAGTG                                                                                        >  SRR4302545.1430128/1‑94 (MQ=60)
       CTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGGCGGGGGAATTACCGCTGCGGTAGGG                                                                                        >  SRR4302545.9571503/1‑94 (MQ=60)
       CTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAACGGCGTCGGGGGAATTACCGCTGCGGTAGTG                                                                                        >  SRR4302545.4980907/1‑94 (MQ=60)
        TCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGC                                                                                       <  SRR4302545.10236561/94‑1 (MQ=60)
        TCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGC                                                                                       <  SRR4302545.10431993/94‑1 (MQ=60)
        TCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGC                                                                                       <  SRR4302545.7920346/94‑1 (MQ=60)
         CTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCG                                                                                      <  SRR4302545.9395232/94‑1 (MQ=60)
         CTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCG                                                                                      >  SRR4302545.1691898/1‑94 (MQ=60)
         CTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCG                                                                                      <  SRR4302545.6915414/94‑1 (MQ=60)
         CTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCG                                                                                      <  SRR4302545.5212604/94‑1 (MQ=60)
         CTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCG                                                                                      <  SRR4302545.393190/94‑1 (MQ=60)
          TGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGC                                                                                     >  SRR4302545.3586416/1‑94 (MQ=60)
          TGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGGAGTGCGC                                                                                     >  SRR4302545.1779530/1‑94 (MQ=60)
           GACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGGT                                                                                    >  SRR4302545.1172825/1‑94 (MQ=60)
            ACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTA                                                                                   <  SRR4302545.3948910/94‑1 (MQ=60)
            ACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCCGCGGGAGTGCGCTA                                                                                   >  SRR4302545.6067931/1‑94 (MQ=60)
             CGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTAC                                                                                  <  SRR4302545.456911/94‑1 (MQ=60)
              GACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGGAATTACCGCTGCGGTAGTGCGCTACT                                                                                 >  SRR4302545.3490879/1‑94 (MQ=60)
              GACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGGAATTACCGCTGCGGTAGTGCGCTACT                                                                                 >  SRR4302545.4185065/1‑94 (MQ=60)
              GACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGGAATTACCGCTGCGGGAGTGCGCTACT                                                                                 >  SRR4302545.2093441/1‑94 (MQ=60)
              CACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACT                                                                                 <  SRR4302545.8020407/94‑1 (MQ=60)
                CGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTAC                                                                               <  SRR4302545.1136400/94‑1 (MQ=60)
                CGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTAC                                                                               >  SRR4302545.1455679/1‑94 (MQ=60)
                CGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTAC                                                                               <  SRR4302545.3071434/94‑1 (MQ=60)
                CGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTAC                                                                               <  SRR4302545.1313261/94‑1 (MQ=60)
                 GGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACG                                                                              <  SRR4302545.7681273/94‑1 (MQ=60)
                  GGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGG                                                                             <  SRR4302545.2415573/94‑1 (MQ=60)
                  GGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGG                                                                             <  SRR4302545.3765414/94‑1 (MQ=60)
                   GGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGC                                                                            <  SRR4302545.7850877/94‑1 (MQ=60)
                    GAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCG                                                                           <  SRR4302545.2247302/94‑1 (MQ=60)
                    GAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCG                                                                           <  SRR4302545.7415200/94‑1 (MQ=60)
                    GAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCG                                                                           >  SRR4302545.1181824/1‑94 (MQ=60)
                     AGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGTGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGG                                                                          <  SRR4302545.10752822/94‑1 (MQ=60)
                     AGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGG                                                                          <  SRR4302545.2003205/94‑1 (MQ=60)
                     AGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGG                                                                          <  SRR4302545.5661957/94‑1 (MQ=60)
                     AGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGG                                                                          <  SRR4302545.10249880/94‑1 (MQ=60)
                     AGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGG                                                                          <  SRR4302545.5715883/94‑1 (MQ=60)
                     AGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTAACGCTGCGGTAGTGCGCTACTACGGCGG                                                                          >  SRR4302545.5860544/1‑94 (MQ=60)
                        CGGCGGGAACGGCAGGTAAACCGATGCTCGCCCCGCTAATGGGCAGCGGCGTCGGGGGAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCAT                                                                       >  SRR4302545.8435172/1‑94 (MQ=60)
                        CGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCAT                                                                       >  SRR4302545.6308980/1‑94 (MQ=60)
                         GGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGGCGCGTCGGGGAAATTACCGCTTCGGTAGGGCGCTACTACGGCGGCATA                                                                      >  SRR4302545.7348142/1‑94 (MQ=60)
                         GGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATT                                                                      >  SRR4302545.3040558/1‑94 (MQ=60)
                         GGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATA                                                                      <  SRR4302545.342056/94‑1 (MQ=60)
                          GCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATAT                                                                     <  SRR4302545.10540664/94‑1 (MQ=60)
                           CGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATT                                                                    >  SRR4302545.3328750/1‑94 (MQ=60)
                           CGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATT                                                                    >  SRR4302545.9230162/1‑94 (MQ=60)
                           CGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATT                                                                    >  SRR4302545.3328751/1‑94 (MQ=60)
                           CGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATT                                                                    <  SRR4302545.2823293/94‑1 (MQ=60)
                           CGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATT                                                                    <  SRR4302545.2823613/94‑1 (MQ=60)
                            GGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTG                                                                   >  SRR4302545.9519635/1‑94 (MQ=60)
                             GGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGC                                                                  >  SRR4302545.3132729/1‑94 (MQ=60)
                              GAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCT                                                                 <  SRR4302545.6086916/94‑1 (MQ=60)
                               AACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTT                                                                <  SRR4302545.6405189/94‑1 (MQ=60)
                               AACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTT                                                                <  SRR4302545.5724954/94‑1 (MQ=60)
                                ACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTG                                                               <  SRR4302545.5151576/94‑1 (MQ=60)
                                ACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTG                                                               >  SRR4302545.3889247/1‑94 (MQ=60)
                                 CGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGG                                                              <  SRR4302545.1365137/94‑1 (MQ=60)
                                  GGCAGGTAAACCGATGCTCGCCTAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGC                                                             >  SRR4302545.6946908/1‑94 (MQ=60)
                                  GGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGC                                                             >  SRR4302545.4589353/1‑94 (MQ=60)
                                  GGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGC                                                             >  SRR4302545.5525889/1‑94 (MQ=60)
                                   GCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCA                                                            <  SRR4302545.10229808/94‑1 (MQ=60)
                                   GCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCA                                                            <  SRR4302545.3691686/94‑1 (MQ=60)
                                    CAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCAC                                                           <  SRR4302545.1286467/94‑1 (MQ=60)
                                     AGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACC                                                          >  SRR4302545.9391960/1‑94 (MQ=60)
                                     AGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACC                                                          >  SRR4302545.5082212/1‑94 (MQ=60)
                                      GGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTGCGGCGGCATATTGCTTGGCACCG                                                         >  SRR4302545.5969025/1‑94 (MQ=60)
                                      GGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCG                                                         <  SRR4302545.6715012/94‑1 (MQ=60)
                                      GGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCGGCGGTAGTGCGCTACTACGGCGGCATATGGCTTGGCACCG                                                         >  SRR4302545.8322532/1‑94 (MQ=60)
                                      GATAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCG                                                         <  SRR4302545.10201824/94‑1 (MQ=60)
                                       GTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGG                                                        >  SRR4302545.3345153/1‑94 (MQ=60)
                                       GTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGG                                                        >  SRR4302545.8871059/1‑94 (MQ=60)
                                        TAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGG                                                       >  SRR4302545.6280776/1‑94 (MQ=60)
                                         AAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGTGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTG                                                      <  SRR4302545.2438774/94‑1 (MQ=60)
                                         AAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGTCGGCATATTTCTTGGCACCGGTG                                                      >  SRR4302545.6218105/1‑94 (MQ=60)
                                         AAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTG                                                      <  SRR4302545.8230542/94‑1 (MQ=60)
                                         AAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTG                                                      <  SRR4302545.8508947/94‑1 (MQ=60)
                                         AAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTG                                                      >  SRR4302545.379785/1‑94 (MQ=60)
                                          AACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGG                                                     <  SRR4302545.913237/94‑1 (MQ=60)
                                          AACAGATCCTCGCCCAGCTAATGCGCAGCGGCGTCGTGGAAATTCCCGCTGCGGTAGTGCGCTACTAGGGCGACATATTGCTTGGCACCGGTGC                                                     <  SRR4302545.8982697/94‑1 (MQ=60)
                                           ACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGG                                                    <  SRR4302545.6719241/94‑1 (MQ=60)
                                           ACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGG                                                    <  SRR4302545.1891474/94‑1 (MQ=60)
                                           ACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGG                                                    >  SRR4302545.2620254/1‑94 (MQ=60)
                                           ACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGG                                                    <  SRR4302545.9518027/94‑1 (MQ=60)
                                           ACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCAACGGTGGG                                                    >  SRR4302545.4829542/1‑94 (MQ=60)
                                            CCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGT                                                   >  SRR4302545.2344570/1‑94 (MQ=60)
                                            CCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGT                                                   <  SRR4302545.10853767/94‑1 (MQ=60)
                                             CGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTT                                                  <  SRR4302545.6460155/94‑1 (MQ=60)
                                             CGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTT                                                  >  SRR4302545.6533300/1‑94 (MQ=60)
                                             CGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTT                                                  >  SRR4302545.7683465/1‑94 (MQ=60)
                                             CGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGGT                                                  >  SRR4302545.810381/1‑94 (MQ=60)
                                              GATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTA                                                 >  SRR4302545.3245729/1‑94 (MQ=60)
                                              GATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTA                                                 <  SRR4302545.10215097/94‑1 (MQ=60)
                                              GATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTA                                                 <  SRR4302545.577782/94‑1 (MQ=60)
                                                  CTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGA                                             >  SRR4302545.3213449/1‑94 (MQ=60)
                                                   TCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAA                                            <  SRR4302545.6458032/94‑1 (MQ=60)
                                                    CGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAA                                           >  SRR4302545.3623885/1‑94 (MQ=60)
                                                    CGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAA                                           <  SRR4302545.4530737/94‑1 (MQ=60)
                                                    CGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAA                                           >  SRR4302545.1747518/1‑94 (MQ=60)
                                                    CGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAA                                           <  SRR4302545.7804270/94‑1 (MQ=60)
                                                    CGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGCAGTGCGCTACTACGGCCGCATATTGCTTGGCACCGGTGGGTTAGTGAAA                                           >  SRR4302545.6068725/1‑94 (MQ=60)
                                                     GCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAG                                          <  SRR4302545.1864676/94‑1 (MQ=60)
                                                     GCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAG                                          <  SRR4302545.613469/94‑1 (MQ=60)
                                                     CCCCAGATAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAG                                          <  SRR4302545.3007806/94‑1 (MQ=60)
                                                      CCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGC                                         >  SRR4302545.8286980/1‑94 (MQ=60)
                                                      CCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAAC                                         >  SRR4302545.3418237/1‑94 (MQ=60)
                                                      CCCAGATACTGGGCAGCGGCGTCGGGGCACTTAGCGCTGCGGTAACGCGCTACTACGGCGGCCTATTGCTTGGCACCGGTGGGTTCGTGAAAGC                                         >  SRR4302545.7353808/1‑94 (MQ=44)
                                                       CCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCG                                        >  SRR4302545.3698480/1‑94 (MQ=60)
                                                       CCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCG                                        <  SRR4302545.10890702/94‑1 (MQ=60)
                                                       CCAGATAGTGGGTAGCGGCGTCGGGGAAATTACTGCTGCGGTAGCGCGCTACTACGGCGGCATATGGGTTGGTACCGGTGGGggagtgggagcg                                        >  SRR4302545.5369478/1‑82 (MQ=36)
                                                        CAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTaaggaaaaggga                                       >  SRR4302545.10748993/1‑82 (MQ=60)
                                                        CAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGT                                       <  SRR4302545.4387572/94‑1 (MQ=60)
                                                         AGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTA                                      <  SRR4302545.5620313/94‑1 (MQ=60)
                                                         AGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTA                                      >  SRR4302545.10619837/1‑94 (MQ=60)
                                                         AGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTA                                      >  SRR4302545.2461173/1‑94 (MQ=60)
                                                          GCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTAT                                     <  SRR4302545.7517642/94‑1 (MQ=60)
                                                          GCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTAT                                     <  SRR4302545.8561296/94‑1 (MQ=60)
                                                           CTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATG                                    <  SRR4302545.1435673/94‑1 (MQ=60)
                                                           CTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATG                                    >  SRR4302545.3565613/1‑94 (MQ=60)
                                                           CTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATG                                    <  SRR4302545.5164793/94‑1 (MQ=60)
                                                           CTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATG                                    >  SRR4302545.2846358/1‑94 (MQ=60)
                                                           CTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATG                                    <  SRR4302545.1055670/94‑1 (MQ=60)
                                                           CTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATG                                    >  SRR4302545.6426120/1‑94 (MQ=60)
                                                           CTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATG                                    >  SRR4302545.6426121/1‑94 (MQ=60)
                                                           CTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGCTAGTGAACGCGTACG                                    >  SRR4302545.3375337/1‑94 (MQ=60)
                                                            TAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATTTTGCTTGGCACCGGTGGGGTAGTGAAAGCGTATGG                                   >  SRR4302545.8249184/1‑94 (MQ=60)
                                                            TAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGG                                   >  SRR4302545.9716667/1‑94 (MQ=60)
                                                              ATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCG                                 <  SRR4302545.10043491/94‑1 (MQ=60)
                                                              ATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCG                                 <  SRR4302545.6384466/94‑1 (MQ=60)
                                                              ATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCG                                 >  SRR4302545.3788154/1‑94 (MQ=60)
                                                               TGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGG                                <  SRR4302545.9512603/94‑1 (MQ=60)
                                                                GGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGC                               <  SRR4302545.5451969/94‑1 (MQ=60)
                                                                 GGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCG                              >  SRR4302545.9937380/1‑94 (MQ=60)
                                                                 GGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCG                              >  SRR4302545.876568/1‑94 (MQ=60)
                                                                 GGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCG                              <  SRR4302545.663554/94‑1 (MQ=60)
                                                                    AGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCG                           >  SRR4302545.396837/1‑94 (MQ=60)
                                                                    AGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCCGCGGCG                           >  SRR4302545.5473197/1‑94 (MQ=60)
                                                                      CGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTG                         <  SRR4302545.2669994/94‑1 (MQ=60)
                                                                      CGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTG                         <  SRR4302545.1201553/94‑1 (MQ=60)
                                                                      CGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTG                         >  SRR4302545.4895305/1‑94 (MQ=60)
                                                                       GGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGA                        <  SRR4302545.523846/94‑1 (MQ=60)
                                                                       GGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGA                        <  SRR4302545.1047507/94‑1 (MQ=60)
                                                                       GGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGA                        >  SRR4302545.7177751/1‑94 (MQ=60)
                                                                       GGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTAGGGCGGTGGCGTGA                        >  SRR4302545.7738303/1‑94 (MQ=60)
                                                                       GGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGGTAGTGAAAGCGTATGGGGGCGGCGTGA                        >  SRR4302545.7738302/1‑94 (MQ=60)
                                                                        GCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAA                       <  SRR4302545.3643344/94‑1 (MQ=60)
                                                                         CGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAAT                      >  SRR4302545.5494898/1‑94 (MQ=60)
                                                                         CGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAAT                      <  SRR4302545.10880323/94‑1 (MQ=60)
                                                                         CGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAAT                      >  SRR4302545.4395964/1‑94 (MQ=60)
                                                                          GTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATC                     <  SRR4302545.1063466/94‑1 (MQ=60)
                                                                          GTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATC                     <  SRR4302545.3968147/94‑1 (MQ=60)
                                                                          GTCGGGGAAATTACCGCTGCGGTAGAGCGCTACTACGGCGGCATATTCCTTGGCACCGGTGGGataggcatggcgtatcgaggacgcgggaatc                     >  SRR4302545.1684385/1‑63 (MQ=60)
                                                                           TCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCA                    >  SRR4302545.1545519/1‑94 (MQ=60)
                                                                           GCGGGGAAATTACCGCCGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCA                    <  SRR4302545.7355367/94‑1 (MQ=60)
                                                                            CGGGGAAATTACCGCTGCGGTAGGGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGATAGTGAAAGCGTATGGGGGCGGCGGGAAGCAG                   >  SRR4302545.2158217/1‑94 (MQ=60)
                                                                             GGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCCCCGGTGGGTTAGGGAAAGCGTATGGCGGGGGCGTGAATCAGG                  >  SRR4302545.4721080/1‑94 (MQ=60)
                                                                             GGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGCCGTGAAGCAGG                  >  SRR4302545.3200973/1‑94 (MQ=60)
                                                                              GGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGC                 <  SRR4302545.1995925/94‑1 (MQ=60)
                                                                              GGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGC                 <  SRR4302545.904132/94‑1 (MQ=60)
                                                                              GGGAAATAACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGC                 >  SRR4302545.10583821/1‑94 (MQ=60)
                                                                              GGGAAAGTACCCCTGCGGTAGTGCCCTACTACGGCCGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGC                 <  SRR4302545.9257382/94‑1 (MQ=60)
                                                                               GGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGGGTATGGggggggggggaacacgggg                >  SRR4302545.9263805/1‑75 (MQ=60)
                                                                               GGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCG                <  SRR4302545.5595429/94‑1 (MQ=60)
                                                                               GGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCG                >  SRR4302545.2571845/1‑94 (MQ=60)
                                                                               GGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCG                >  SRR4302545.8518293/1‑94 (MQ=60)
                                                                               GGAAATTACCGCGGCGGTATTACGCTAGTACGGCGGCATATTGCTTGTCACCGGTGGTTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCG                <  SRR4302545.7639557/94‑1 (MQ=60)
                                                                                GAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGC               >  SRR4302545.10361987/1‑94 (MQ=60)
                                                                                GAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGC               <  SRR4302545.4150384/94‑1 (MQ=60)
                                                                                 AAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCT              >  SRR4302545.9847988/1‑94 (MQ=60)
                                                                                 AAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGCGGGTTAGTGAAAGCGTTTGGCGGGGGCGGGAATCAGGCGCC              >  SRR4302545.3744058/1‑94 (MQ=60)
                                                                                  AATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTG             >  SRR4302545.8447825/1‑94 (MQ=60)
                                                                                  AATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCAACGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTG             >  SRR4302545.2656955/1‑94 (MQ=60)
                                                                                   ATTACCGCTGCGGTAGTGCGCTACTACGGCAGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGC            >  SRR4302545.4980709/1‑94 (MQ=60)
                                                                                    TTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCG           <  SRR4302545.355624/94‑1 (MQ=60)
                                                                                     TACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGGGGCGGGAATAAGGGGCTGCGC          >  SRR4302545.2205668/1‑94 (MQ=60)
                                                                                     TACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGGGAAACAGGCGCTGCGC          >  SRR4302545.2077650/1‑94 (MQ=60)
                                                                                      ACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCC         >  SRR4302545.2539072/1‑94 (MQ=60)
                                                                                      ACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCC         >  SRR4302545.4741293/1‑94 (MQ=60)
                                                                                         GCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGC      >  SRR4302545.7181525/1‑94 (MQ=60)
                                                                                          CTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCT     >  SRR4302545.7754346/1‑94 (MQ=60)
                                                                                          CTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCT     <  SRR4302545.8837386/94‑1 (MQ=60)
                                                                                          CTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCT     <  SRR4302545.2409053/94‑1 (MQ=60)
                                                                                            GCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCTAA   <  SRR4302545.606412/94‑1 (MQ=60)
                                                                                            GCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCTAA   <  SRR4302545.10132894/94‑1 (MQ=60)
                                                                                            GCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCTAA   >  SRR4302545.170277/1‑94 (MQ=60)
                                                                                             CGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCTAAC  <  SRR4302545.1521086/94‑1 (MQ=60)
                                                                                             CGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCTAAC  >  SRR4302545.1007512/1‑94 (MQ=60)
                                                                                             |                                                                                             
TGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCTAAC  >  NC_000913/4030281‑4030467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑