Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,030,374 T→C V98A (GTG→GCG)  b3848 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,030,3740TC100.0% 771.4 / NA 222V98A (GTG→GCG) b3848hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (104/118);  total (104/118)

Only 100 of 235 total aligned reads displayed.

GTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGC  >  NC_000913/4030284‑4030463
                                                                                          |                                                                                         
gTTTCTCTTACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCgg                                                                                         >  1:4833376/1‑93 (MQ=255)
gTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCgg                                                                                         <  1:4617514/93‑1 (MQ=255)
gTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAACCGATGCTCGCCCCGCTTATGTGGAGCGGGGTCGGGGAAAGTGCCGCTgggg                                                                                         >  2:2291908/1‑93 (MQ=255)
 tttCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGt                                                                                        <  1:1137251/93‑1 (MQ=255)
 tttCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGt                                                                                        <  1:5471292/93‑1 (MQ=255)
 tttCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAACCGATGCTCGCCCACCTAATCGGCAGCGGCGTCGGGGAAATTACCGCTGCGGt                                                                                        >  1:6139445/1‑93 (MQ=255)
   tctcTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAg                                                                                      <  2:27840/93‑1 (MQ=255)
   tctcTGACGACGCGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCATCGGCGTCGGGGAAATTGCCGCCGCGGTAg                                                                                      <  2:833154/93‑1 (MQ=255)
     tctGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGGAGTg                                                                                    >  2:3560356/1‑93 (MQ=255)
      ctGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTgc                                                                                   <  1:5845086/93‑1 (MQ=255)
      ctGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTgc                                                                                   <  2:778838/93‑1 (MQ=255)
      ctGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTgc                                                                                   <  2:6034397/93‑1 (MQ=255)
      ctGACGACGGGGAGCCGGCGGGAACGGCAGGGAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGGCGGGGAAATTACCGCTGCGGGAGTgc                                                                                   >  1:3073293/1‑93 (MQ=255)
        gacgacGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTgcgc                                                                                 <  2:244358/93‑1 (MQ=255)
        gacgacGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTgcgc                                                                                 <  2:12018/93‑1 (MQ=255)
         acgacgGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGct                                                                                <  1:4112199/93‑1 (MQ=255)
           gacgGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGctac                                                                              <  1:3937955/93‑1 (MQ=255)
            acgGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGGAATTACCGCTGCGGTAGTGCGctact                                                                             >  2:2757355/1‑93 (MQ=255)
            acgGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGctact                                                                             >  1:5176696/1‑93 (MQ=255)
            acgGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATAGGGAGCGGCGTCGGGGGAATTACCGCTGCGGTAGTGCGctact                                                                             >  2:4047352/1‑93 (MQ=255)
             cgGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGctacta                                                                            <  1:5959755/93‑1 (MQ=255)
              ggggAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCtactac                                                                           <  1:644500/93‑1 (MQ=255)
              ggggAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCtactac                                                                           <  1:1292765/93‑1 (MQ=255)
               gggAGCCGGCGGGAACGGCAGGGAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTAACCCTGCGGTAGTGCGCTCCTAcg                                                                          >  1:5708279/1‑93 (MQ=255)
                 gAGCCGGCGGGAACGGCAGGTAAACCGAAGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGGAATTACCGCTGCGGTAGTGCGCTACCAcggg                                                                        >  2:5894806/1‑92 (MQ=255)
                   gCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTAcggcgg                                                                      >  2:1594361/1‑93 (MQ=255)
                    ccGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACcgcggc                                                                     >  1:4649579/1‑93 (MQ=255)
                     cggcggGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCa                                                                    >  2:2625055/1‑93 (MQ=255)
                      ggcggGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCat                                                                   >  1:3798459/1‑93 (MQ=255)
                      ggcggGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCat                                                                   <  1:1600909/93‑1 (MQ=255)
                       gcggGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCata                                                                  <  1:1751835/93‑1 (MQ=255)
                       gcggGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCata                                                                  <  1:1472862/93‑1 (MQ=255)
                         gggAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATAtt                                                                <  1:5628441/93‑1 (MQ=255)
                         gggAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATAtt                                                                <  2:4958123/93‑1 (MQ=255)
                               ggCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTgg                                                          <  2:4805056/93‑1 (MQ=255)
                                gCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGc                                                         <  1:2013679/93‑1 (MQ=255)
                                   ggTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCAcc                                                      >  1:6261888/1‑93 (MQ=255)
                                   ggTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCAcc                                                      <  2:4438113/93‑1 (MQ=255)
                                    gTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCg                                                     <  1:1909204/93‑1 (MQ=255)
                                    gTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCg                                                     >  1:3656340/1‑93 (MQ=255)
                                     tAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCgg                                                    <  2:5871633/93‑1 (MQ=255)
                                      aagccGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGt                                                   <  1:698638/90‑1 (MQ=255)
                                      aaaCCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGt                                                   <  2:4971680/93‑1 (MQ=255)
                                       aaCCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTg                                                  >  1:2808630/1‑93 (MQ=255)
                                       aaCCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTg                                                  <  2:2346535/93‑1 (MQ=255)
                                        aCCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTgg                                                 >  1:6218742/1‑93 (MQ=255)
                                        aCCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTgg                                                 <  2:5467922/93‑1 (MQ=255)
                                         ccGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTggg                                                <  1:4403084/93‑1 (MQ=255)
                                         ccGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTggg                                                <  1:4934285/93‑1 (MQ=255)
                                          cGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGt                                               >  1:3916848/1‑93 (MQ=255)
                                           gATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGtt                                              >  1:1959765/1‑93 (MQ=255)
                                            aTGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTa                                             <  1:6386056/93‑1 (MQ=255)
                                            aTGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTa                                             >  2:4183829/1‑93 (MQ=255)
                                            aTGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTa                                             <  2:1066273/93‑1 (MQ=255)
                                            aTGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGGTa                                             >  2:5576326/1‑93 (MQ=255)
                                             tGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAg                                            >  1:4802874/1‑93 (MQ=255)
                                             tGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAg                                            >  1:2407481/1‑93 (MQ=255)
                                             tGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAg                                            >  2:4278760/1‑93 (MQ=255)
                                              gCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGt                                           <  2:4483866/93‑1 (MQ=255)
                                               cTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTg                                          >  1:2137058/1‑93 (MQ=255)
                                                tCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGa                                         >  2:5752481/1‑93 (MQ=255)
                                                tCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGa                                         >  1:912606/1‑93 (MQ=255)
                                                 cGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGaa                                        >  2:5228991/1‑93 (MQ=255)
                                                  gCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGaaa                                       <  1:235407/93‑1 (MQ=255)
                                                  gCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGaaa                                       <  1:1305178/93‑1 (MQ=255)
                                                     cAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCg                                    >  1:4045693/1‑93 (MQ=255)
                                                      aGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGt                                   >  1:1007165/1‑93 (MQ=255)
                                                       gCTAATGGGCAGCGGCGTCGGGTAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTa                                  <  2:1094911/93‑1 (MQ=255)
                                                        cTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTAt                                 <  1:1194709/93‑1 (MQ=255)
                                                         tAATGGTCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATg                                <  2:3184735/93‑1 (MQ=255)
                                                          aaTGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATgg                               >  1:4355357/1‑93 (MQ=255)
                                                              ggCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATggcggg                           >  2:6069570/1‑92 (MQ=255)
                                                              ggCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATggcggc                           <  1:2259800/93‑1 (MQ=255)
                                                                cAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATggcggcgg                         >  2:620909/1‑93 (MQ=255)
                                                                 aGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATggcggcggc                        <  2:293551/93‑1 (MQ=255)
                                                                  gcggcgTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGgcggcggcg                       >  2:198549/1‑93 (MQ=255)
                                                                  gcggcgTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGgcggcggcg                       >  1:2625690/1‑93 (MQ=255)
                                                                     gcgTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAGGCGTATGGCGGCGGCGTGa                    <  2:6321189/93‑1 (MQ=255)
                                                                        tCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATc                 >  2:5783220/1‑93 (MQ=255)
                                                                        tCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGGTATTGAAAGCGTATGGCAGCGGCGTGAATc                 >  1:3129438/1‑93 (MQ=255)
                                                                         cGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCa                <  2:2101592/93‑1 (MQ=255)
                                                                         cGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCa                <  1:1143717/93‑1 (MQ=255)
                                                                         cGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCa                <  2:128680/93‑1 (MQ=255)
                                                                          ggggAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAg               <  1:3417006/93‑1 (MQ=255)
                                                                           gggAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAgg              >  2:1840680/1‑93 (MQ=255)
                                                                            ggAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGgc             >  2:1498684/1‑93 (MQ=255)
                                                                            ggAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGgc             >  1:2900614/1‑93 (MQ=255)
                                                                            ggAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGgc             >  2:3873081/1‑93 (MQ=255)
                                                                             gAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGgcg            >  1:954921/1‑93 (MQ=255)
                                                                              aaaTTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGgcgc           <  1:3673006/93‑1 (MQ=255)
                                                                              aaaTTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGgcgc           <  1:5039537/93‑1 (MQ=255)
                                                                               aaTTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCt          >  2:1436673/1‑93 (MQ=255)
                                                                                aTTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTg         >  2:1248820/1‑93 (MQ=255)
                                                                                 ttACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTgc        >  2:5494229/1‑93 (MQ=255)
                                                                                 ttACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTgc        <  1:4087806/93‑1 (MQ=255)
                                                                                 ttACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTgc        <  1:2657906/93‑1 (MQ=255)
                                                                                    ccGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGcc     >  2:3406838/1‑93 (MQ=255)
                                                                                    ccGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGcc     >  2:3143609/1‑93 (MQ=255)
                                                                                     cGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCa    >  1:3227955/1‑93 (MQ=255)
                                                                                     cGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCa    <  1:5371103/93‑1 (MQ=255)
                                                                                          |                                                                                         
GTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGC  >  NC_000913/4030284‑4030463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCTAAC  >  NC_000913/4030281‑4030467
                                                                                             |                                                                                             
TGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAACCGAAGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGGAATTACCGCTGC                                                                                               >  SRR4302537.4541307/1‑94 (MQ=60)
 GGGTGTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCG                                                                                              <  SRR4302537.3215606/94‑1 (MQ=60)
  GGTTTCTCTTACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGG                                                                                             >  SRR4302537.4866751/1‑94 (MQ=60)
   GTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGT                                                                                            <  SRR4302537.5170968/94‑1 (MQ=60)
   GTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGT                                                                                            <  SRR4302537.4649495/94‑1 (MQ=60)
   GTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGT                                                                                            <  SRR4302537.2006202/94‑1 (MQ=60)
   GTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAACCGATGCTCGCCCACCTAATCGGCAGCGGCGTCGGGGAAATTACCGCTGCGGT                                                                                            >  SRR4302537.6181659/1‑94 (MQ=60)
    TTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTA                                                                                           <  SRR4302537.1145152/94‑1 (MQ=60)
    TTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTA                                                                                           <  SRR4302537.5508621/94‑1 (MQ=60)
     TTCTCTGACGACGGGGAGCCGGCGGGAACTGCAGGTAAACCGATGATCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAG                                                                                          >  SRR4302537.2395205/1‑94 (MQ=60)
     TTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAG                                                                                          <  SRR4302537.2376459/94‑1 (MQ=60)
       CTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTG                                                                                        <  SRR4302537.3745050/94‑1 (MQ=60)
       CTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTG                                                                                        <  SRR4302537.406892/94‑1 (MQ=60)
       CTCTGACGACGGGGAGCCGGCGGGAACGGCAGGCAAACCGATGCTCGCCCAGCTAATAGGCAGCGGCGTCGGGGAAATTACCGGTGCGGTAGTG                                                                                        >  SRR4302537.3320195/1‑94 (MQ=60)
        TCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGC                                                                                       <  SRR4302537.1302406/94‑1 (MQ=60)
        TCTGACGACGGGGAGCCGGCGGGAACGGCAGGGAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGGCGGGGAAATTACCGCTGCGGGAGTGC                                                                                       >  SRR4302537.3094704/1‑94 (MQ=60)
         CTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCG                                                                                      <  SRR4302537.5911363/94‑1 (MQ=60)
         CTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCG                                                                                      >  SRR4302537.4033389/1‑94 (MQ=60)
         CTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCG                                                                                      <  SRR4302537.5885166/94‑1 (MQ=60)
           GACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCT                                                                                    <  SRR4302537.2663260/94‑1 (MQ=60)
           GACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCT                                                                                    <  SRR4302537.2786789/94‑1 (MQ=60)
            ACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTA                                                                                   <  SRR4302537.4140923/94‑1 (MQ=60)
             CGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGGAATTACCGCTGCGGTAGTGCGCTAC                                                                                  >  SRR4302537.5369749/1‑94 (MQ=60)
              GACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACT                                                                                 <  SRR4302537.3965484/94‑1 (MQ=60)
              GACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACT                                                                                 >  SRR4302537.5212322/1‑94 (MQ=60)
                CGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTAC                                                                               <  SRR4302537.6000661/94‑1 (MQ=60)
                CGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTAC                                                                               >  SRR4302537.3550013/1‑94 (MQ=60)
                 GGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACG                                                                              <  SRR4302537.648887/94‑1 (MQ=60)
                 GGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACG                                                                              <  SRR4302537.1445120/94‑1 (MQ=60)
                 GGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACG                                                                              <  SRR4302537.1301755/94‑1 (MQ=60)
                 GGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACG                                                                              <  SRR4302537.4394523/94‑1 (MQ=60)
                 GGGGAGCCGGCGGGAACGGCAGGGAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTAACCCTGCGGTAGTGCGCTCCTACG                                                                              >  SRR4302537.5747341/1‑94 (MQ=60)
                  GGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGGAATTACCGCTGCGGGAGTGCGCTAATACGG                                                                             >  SRR4302537.5541540/1‑94 (MQ=60)
                     AGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGG                                                                          >  SRR4302537.5813653/1‑94 (MQ=60)
                      GCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACCGCGGC                                                                         >  SRR4302537.4681724/1‑94 (MQ=60)
                       CCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCA                                                                        <  SRR4302537.6101913/94‑1 (MQ=60)
                       CCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCA                                                                        <  SRR4302537.4790769/94‑1 (MQ=60)
                        CGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCAT                                                                       >  SRR4302537.3959013/1‑94 (MQ=60)
                        CGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCAT                                                                       >  SRR4302537.3825107/1‑94 (MQ=60)
                         GGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATA                                                                      <  SRR4302537.1612102/94‑1 (MQ=60)
                          GCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATAT                                                                     <  SRR4302537.1764046/94‑1 (MQ=60)
                          GCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATAT                                                                     <  SRR4302537.2486245/94‑1 (MQ=60)
                          GCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATAT                                                                     <  SRR4302537.1483098/94‑1 (MQ=60)
                            GGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTG                                                                   <  SRR4302537.5666946/94‑1 (MQ=60)
                              GAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCT                                                                 <  SRR4302537.1890064/94‑1 (MQ=60)
                               AACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTT                                                                <  SRR4302537.2921500/94‑1 (MQ=60)
                               AACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTT                                                                <  SRR4302537.4854043/94‑1 (MQ=60)
                                ACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTG                                                               <  SRR4302537.4659859/94‑1 (MQ=60)
                                ACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTG                                                               <  SRR4302537.2049496/94‑1 (MQ=60)
                                  GGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGC                                                             >  SRR4302537.6439356/1‑94 (MQ=60)
                                   GCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCA                                                            <  SRR4302537.2027716/94‑1 (MQ=60)
                                     AGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACC                                                          <  SRR4302537.326003/94‑1 (MQ=60)
                                     AGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACC                                                          >  SRR4302537.6304877/1‑94 (MQ=60)
                                      GGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCG                                                         >  SRR4302537.3104079/1‑94 (MQ=60)
                                      GGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCG                                                         <  SRR4302537.2398902/94‑1 (MQ=60)
                                      GGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCG                                                         >  SRR4302537.3682072/1‑94 (MQ=60)
                                       GTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTTGCACCGG                                                        >  SRR4302537.1724054/1‑94 (MQ=60)
                                       GTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGG                                                        <  SRR4302537.1922458/94‑1 (MQ=60)
                                        TAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGAAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGT                                                       <  SRR4302537.460078/94‑1 (MQ=60)
                                         AAGCCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTG                                                      <  SRR4302537.703422/94‑1 (MQ=60)
                                         AAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTG                                                      >  SRR4302537.2828265/1‑94 (MQ=60)
                                          AACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGG                                                     >  SRR4302537.6261558/1‑94 (MQ=60)
                                          AACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGG                                                     <  SRR4302537.1941759/94‑1 (MQ=60)
                                          AACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGG                                                     >  SRR4302537.934054/1‑94 (MQ=60)
                                            CCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGT                                                   <  SRR4302537.4433468/94‑1 (MQ=60)
                                            CCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGT                                                   >  SRR4302537.5081672/1‑94 (MQ=60)
                                            CCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGT                                                   >  SRR4302537.3944226/1‑94 (MQ=60)
                                            CCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGT                                                   <  SRR4302537.4968319/94‑1 (MQ=60)
                                             CGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTT                                                  <  SRR4302537.2297540/94‑1 (MQ=60)
                                             CGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTT                                                  >  SRR4302537.5909393/1‑94 (MQ=60)
                                             CGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTT                                                  >  SRR4302537.1973397/1‑94 (MQ=60)
                                               CTGCTCGCCCAGCTAATGGGCGGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTCCTACGGCGGCATATTGCTTGGCGCCGGTGGGTTAG                                                <  SRR4302537.4620709/94‑1 (MQ=60)
                                               ATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAG                                                <  SRR4302537.6429580/94‑1 (MQ=60)
                                               ATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAG                                                >  SRR4302537.3690682/1‑94 (MQ=60)
                                               ATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAG                                                >  SRR4302537.2424427/1‑94 (MQ=60)
                                               ATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAG                                                >  SRR4302537.4836007/1‑94 (MQ=60)
                                                TGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTCGGCACCGGTGGGTTAGT                                               <  SRR4302537.1861010/94‑1 (MQ=60)
                                                 GCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTG                                              >  SRR4302537.2152104/1‑94 (MQ=60)
                                                  CTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGA                                             >  SRR4302537.918902/1‑94 (MQ=60)
                                                  CTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGA                                             >  SRR4302537.6407931/1‑94 (MQ=60)
                                                    CGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAA                                           <  SRR4302537.3234109/94‑1 (MQ=60)
                                                     GCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAG                                          <  SRR4302537.1314251/94‑1 (MQ=60)
                                                     GCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAG                                          <  SRR4302537.1178059/94‑1 (MQ=60)
                                                     GCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAG                                          <  SRR4302537.237067/94‑1 (MQ=60)
                                                     GCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAG                                          <  SRR4302537.4958246/94‑1 (MQ=60)
                                                      CCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGGTAGTGAAAGG                                         >  SRR4302537.759670/1‑94 (MQ=60)
                                                       CCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCG                                        >  SRR4302537.4073981/1‑94 (MQ=60)
                                                       CCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCG                                        >  SRR4302537.4653379/1‑94 (MQ=60)
                                                        CAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGT                                       >  SRR4302537.1014117/1‑94 (MQ=60)
                                                          GCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTAT                                     <  SRR4302537.2217169/94‑1 (MQ=60)
                                                           CTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATG                                    <  SRR4302537.1203005/94‑1 (MQ=60)
                                                            TAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGG                                   <  SRR4302537.5701468/94‑1 (MQ=60)
                                                            TAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGG                                   >  SRR4302537.1526132/1‑94 (MQ=60)
                                                            TAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGG                                   <  SRR4302537.4933256/94‑1 (MQ=60)
                                                            TAATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGG                                   >  SRR4302537.4385508/1‑94 (MQ=60)
                                                              ATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCG                                 >  SRR4302537.5530295/1‑94 (MQ=60)
                                                              ATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCG                                 <  SRR4302537.4992885/94‑1 (MQ=60)
                                                              ATGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCG                                 <  SRR4302537.5411160/94‑1 (MQ=60)
                                                               TGGGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGG                                >  SRR4302537.1225962/1‑94 (MQ=60)
                                                                 GGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCG                              <  SRR4302537.2275748/94‑1 (MQ=60)
                                                                 GGCAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCG                              >  SRR4302537.6499975/1‑94 (MQ=60)
                                                                   CAGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGC                            <  SRR4302537.6286840/94‑1 (MQ=60)
                                                                    AGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCG                           >  SRR4302537.2644086/1‑94 (MQ=60)
                                                                    AGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCG                           >  SRR4302537.3743757/1‑94 (MQ=60)
                                                                    AGCGGCGTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCG                           >  SRR4302537.4135412/1‑94 (MQ=60)
                                                                         CGTCGGGGAAATTACCGCTGCGGGAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGGTAGTGAAAGCGTATGGCGGCGGCGTGAAT                      >  SRR4302537.3912965/1‑94 (MQ=60)
                                                                          GTCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGGTATTGAAAGCGTATGGCAGCGGCGTGAATC                     >  SRR4302537.3151375/1‑94 (MQ=60)
                                                                           TCGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCA                    <  SRR4302537.1198349/94‑1 (MQ=60)
                                                                            CGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAG                   <  SRR4302537.1151670/94‑1 (MQ=60)
                                                                            CGGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGAGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAG                   <  SRR4302537.3395150/94‑1 (MQ=60)
                                                                             GGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGG                  <  SRR4302537.3441197/94‑1 (MQ=60)
                                                                             GGGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGGGAATCAGG                  >  SRR4302537.2037683/1‑94 (MQ=60)
                                                                              GGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGC                 >  SRR4302537.2920991/1‑94 (MQ=60)
                                                                              GGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGC                 >  SRR4302537.3047056/1‑94 (MQ=60)
                                                                              GGGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATAAGGC                 >  SRR4302537.2768520/1‑94 (MQ=60)
                                                                               GGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCG                >  SRR4302537.961512/1‑94 (MQ=60)
                                                                               GGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCG                >  SRR4302537.4114202/1‑94 (MQ=60)
                                                                               GGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCG                >  SRR4302537.2872208/1‑94 (MQ=60)
                                                                               GGAAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATACTGCTTGGCAACGGCGGGTTAGTGCAAGCGTATCGCGGCcgggtgaatcgaggg                >  SRR4302537.6444252/1‑79 (MQ=60)
                                                                                 AAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCT              <  SRR4302537.5074233/94‑1 (MQ=60)
                                                                                 AAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCT              <  SRR4302537.1151486/94‑1 (MQ=60)
                                                                                 AAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCT              <  SRR4302537.4242749/94‑1 (MQ=60)
                                                                                 AAATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCT              <  SRR4302537.3698848/94‑1 (MQ=60)
                                                                                  AATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTG             <  SRR4302537.4269199/94‑1 (MQ=60)
                                                                                   ATTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGC            >  SRR4302537.1033928/1‑94 (MQ=60)
                                                                                    TTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCG           <  SRR4302537.4116354/94‑1 (MQ=60)
                                                                                    TTACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCG           <  SRR4302537.2676480/94‑1 (MQ=60)
                                                                                      ACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCC         <  SRR4302537.6217121/94‑1 (MQ=60)
                                                                                      ACCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCC         >  SRR4302537.1411170/1‑94 (MQ=60)
                                                                                       CCGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCA        >  SRR4302537.3250703/1‑94 (MQ=60)
                                                                                        CGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAG       >  SRR4302537.6491928/1‑94 (MQ=60)
                                                                                        CGCTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAG       <  SRR4302537.5407846/94‑1 (MQ=60)
                                                                                          CTGCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCT     >  SRR4302537.5059477/1‑94 (MQ=60)
                                                                                            GCGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCTAA   <  SRR4302537.169026/94‑1 (MQ=60)
                                                                                             CGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCTAAC  <  SRR4302537.2837099/94‑1 (MQ=60)
                                                                                             CGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCTAAC  <  SRR4302537.5925393/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGGGTTTCTCTGACGACGGGGAGCCGGCGGGAACGGCAGGTAAACCGATGCTCGCCCAGCTAATGGGCAGCGGCGTCGGGGAAATTACCGCTGTGGTAGTGCGCTACTACGGCGGCATATTGCTTGGCACCGGTGGGTTAGTGAAAGCGTATGGCGGCGGCGTGAATCAGGCGCTGCGCCAGCTAAC  >  NC_000913/4030281‑4030467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑