Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,051,144 Δ1 bp intergenic (+146/+305) hemN → / ← glnG O2‑independent coproporphyrinogen III oxidase/GlnG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,051,1430C.99.8% 1675.3 / ‑3.8 501intergenic (+145/+306)hemN/glnGO2‑independent coproporphyrinogen III oxidase/GlnG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base . (262/238);  minor base G (1/0);  total (263/238)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 533 total aligned reads displayed.

TGCTGAGAACAAGATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCCATCTGAGCATGATGCCTGATGCGGCG‑TGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCT  >  NC_000913/4051050‑4051232
                                                                                             |                                                                                          
 gCTGAGAACAAGATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGca                                                                                           <  1:9798228/94‑2 (MQ=255)
       aaCAAGATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAg                                                                                    >  1:1399509/1‑94 (MQ=255)
         cAAGATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCa                                                                                  <  1:5900630/94‑1 (MQ=255)
          aaGATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCat                                                                                 >  2:2435115/1‑94 (MQ=255)
          aaGATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCat                                                                                 >  2:1758175/1‑94 (MQ=255)
          aaGATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCat                                                                                 >  1:3830132/1‑94 (MQ=255)
          aaGATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCat                                                                                 >  1:3562989/1‑94 (MQ=255)
           aGATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCatg                                                                                <  1:548100/94‑1 (MQ=255)
            gATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCatga                                                                               <  2:1380690/94‑1 (MQ=255)
            gATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCatga                                                                               <  2:9003027/94‑1 (MQ=255)
             aTTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCatgat                                                                              <  1:10155207/94‑1 (MQ=255)
              ttGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCatgatg                                                                             <  1:5094520/94‑1 (MQ=255)
                 ccTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCt                                                                          >  1:8704178/1‑94 (MQ=255)
                  cTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTg                                                                         <  1:9495898/94‑1 (MQ=255)
                   tGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGa                                                                        >  1:2337865/1‑94 (MQ=255)
                    gATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGAt                                                                       <  2:6450529/94‑1 (MQ=255)
                    gATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGAt                                                                       <  2:10345617/94‑1 (MQ=255)
                     aTGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATg                                                                      >  1:10010470/1‑94 (MQ=255)
                      tGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgc                                                                     <  1:8199645/94‑1 (MQ=255)
                      tGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgc                                                                     <  2:5855554/94‑1 (MQ=255)
                       gcgcTGGGCAACAAAACCTGTTGAAATTATTTCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgca                                                                    <  1:700707/94‑2 (MQ=255)
                        cgcTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTCGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcgg                                                                   >  1:10392740/1‑94 (MQ=255)
                          cTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcg                                                                 <  2:2522900/94‑1 (MQ=255)
                          cTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcg                                                                 <  2:6138604/94‑1 (MQ=255)
                          cTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcg                                                                 >  2:6890291/1‑94 (MQ=255)
                            gggCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcgct                                                               >  1:42345/1‑92 (MQ=255)
                            gggCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcgct                                                               >  2:25959/1‑92 (MQ=255)
                              gCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcgctgg                                                             <  2:3061257/94‑5 (MQ=255)
                              gCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcgctgg                                                             <  1:3231646/94‑5 (MQ=255)
                                aacaaAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTc                                                           >  2:3776371/1‑94 (MQ=25)
                                aacaaAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTc                                                           >  2:8384756/1‑94 (MQ=25)
                                 acaaAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑GATCTGAGCATGCTGCCTGATGCGGCGCTGGTcg                                                          >  2:2231480/1‑94 (MQ=25)
                                  caaAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTcgc                                                         >  1:8100955/1‑94 (MQ=25)
                                   aaaaCCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTcgcg                                                        >  1:10294758/1‑94 (MQ=25)
                                    aaaCCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGt                                                       >  1:1731558/1‑94 (MQ=25)
                                     aaCCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTc                                                      >  2:10540152/1‑94 (MQ=25)
                                      aCCTGTTGAAATTATTGCTTTTGTAGGCCTGATAAGGCGTTTACGACGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCt                                                     >  2:6256047/1‑94 (MQ=21)
                                      aCCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCt                                                     >  2:3351462/1‑94 (MQ=21)
                                      aCCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCt                                                     <  2:1471512/94‑1 (MQ=25)
                                        cTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTa                                                   <  2:1983306/94‑1 (MQ=21)
                                        cTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTa                                                   <  2:5555695/94‑1 (MQ=21)
                                         tGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTAt                                                  >  1:6500414/1‑94 (MQ=21)
                                         tGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGACGCATCAGGGC‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTAt                                                  >  2:4635041/1‑94 (MQ=21)
                                          gTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATc                                                 >  1:9051698/1‑94 (MQ=21)
                                            tGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAg                                               <  2:8141766/94‑1 (MQ=21)
                                            tGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAg                                               >  2:9173136/1‑94 (MQ=18)
                                            tGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAg                                               <  2:9455215/94‑1 (MQ=21)
                                             gAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAgg                                              <  2:1189589/94‑1 (MQ=18)
                                              aaaTTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGc                                             <  1:6625585/94‑1 (MQ=18)
                                                attcttGCTTTTGTAGGCCGGATAAGGCGTTTACGTCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGcccc                                           >  1:2102114/1‑93 (MQ=17)
                                                attattGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCt                                           >  1:2465448/1‑94 (MQ=18)
                                                 ttattGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTa                                          >  1:6182573/1‑94 (MQ=18)
                                                 ttattGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTa                                          <  1:3438225/94‑1 (MQ=18)
                                                 ttattGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTa                                          <  1:7229738/94‑1 (MQ=18)
                                                 ttattGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CAGATGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTa                                          <  1:3973571/94‑1 (MQ=17)
                                                  tattGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCTTGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTAc                                         <  1:7376910/94‑1 (MQ=17)
                                                  tattGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTAc                                         <  2:9708396/94‑1 (MQ=18)
                                                  tattGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTAc                                         <  2:8679460/94‑1 (MQ=18)
                                                   attGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACg                                        >  2:4985794/1‑94 (MQ=17)
                                                   attGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACg                                        >  1:2912115/1‑94 (MQ=17)
                                                   attGCTTTTGTAGGCCGGATAAGGCGTTTACGCCCCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACg                                        >  1:7762267/1‑94 (MQ=17)
                                                    ttGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCAGCGCTGGTCGCGTCTTATCAGGCCTACGa                                       >  2:7685235/1‑94 (MQ=17)
                                                      gCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACt                                     <  2:7310783/94‑1 (MQ=17)
                                                       cTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACtt                                    >  1:1250883/1‑94 (MQ=18)
                                                        ttttGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACttt                                   <  2:1589435/94‑1 (MQ=18)
                                                         tttGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTtc                                  <  2:562201/94‑1 (MQ=18)
                                                            gTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTcc                               <  2:3679573/94‑1 (MQ=18)
                                                             tAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCg                              >  1:4529308/1‑94 (MQ=18)
                                                             tAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCg                              >  1:4109220/1‑94 (MQ=18)
                                                              aGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGc                             >  2:10010473/1‑94 (MQ=18)
                                                              aGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGc                             >  1:5659264/1‑94 (MQ=18)
                                                               ggCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCa                            <  2:7991301/94‑1 (MQ=18)
                                                               ggCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCa                            >  2:7098040/1‑94 (MQ=18)
                                                                gCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCAt                           <  2:7036616/94‑1 (MQ=18)
                                                                  cGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATcc                         <  1:3050212/94‑1 (MQ=18)
                                                                  cGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATcc                         >  2:7804051/1‑94 (MQ=18)
                                                                  cGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATcc                         >  2:2746419/1‑94 (MQ=18)
                                                                  cGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATcc                         <  1:7067745/94‑1 (MQ=18)
                                                                   ggATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCg                        >  2:6764345/1‑94 (MQ=18)
                                                                    gATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCgg                       <  1:6348464/94‑1 (MQ=18)
                                                                    gATAAGGCAGTTACGCCGCAGCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCgg                       <  1:8301930/94‑1 (MQ=18)
                                                                    gATAAGGAGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCgg                       <  1:6822756/94‑1 (MQ=18)
                                                                     aTAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGc                      >  1:7470644/1‑94 (MQ=21)
                                                                      tAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCa                     >  2:4163874/1‑94 (MQ=21)
                                                                       aaGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCAt                    >  2:3972718/1‑94 (MQ=21)
                                                                       aaGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCAt                    >  1:1868517/1‑94 (MQ=21)
                                                                         ggCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCCTaa                  >  1:4818862/1‑94 (MQ=18)
                                                                          gCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATaaa                 >  1:5280027/1‑94 (MQ=21)
                                                                           cGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAaac                >  1:1576154/1‑94 (MQ=21)
                                                                              ttACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAaacaac             <  1:2766541/94‑1 (MQ=25)
                                                                              ttACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAaacaac             >  1:10551147/1‑94 (MQ=25)
                                                                               tACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACg            >  2:7616741/1‑94 (MQ=25)
                                                                                 cgccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAg          >  2:985269/1‑94 (MQ=25)
                                                                                   ccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCt        <  1:9322073/94‑1 (MQ=25)
                                                                                   ccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCt        <  2:289650/94‑1 (MQ=25)
                                                                                    cgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCtt       <  2:5180129/94‑1 (MQ=25)
                                                                                     gcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTc      <  2:6651588/94‑1 (MQ=25)
                                                                                      cATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCa     >  1:8873726/1‑94 (MQ=37)
                                                                                        tCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGc   <  2:10211300/94‑1 (MQ=37)
                                                                                        tCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGc   >  2:4328401/1‑94 (MQ=38)
                                                                                             |                                                                                          
TGCTGAGAACAAGATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCCATCTGAGCATGATGCCTGATGCGGCG‑TGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCT  >  NC_000913/4051050‑4051232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 289 total aligned reads displayed.
CTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCCA‑TCTGAGCATGATGCCTGATGCGGCG‑TGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAGCACG  >  NC_000913/4051076‑4051263
                                                                                               |                                                                                              
CTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCG                                                                                                >  SRR4302545.2392656/1‑94 (MQ=60)
 TGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCG‑C                                                                                              >  SRR4302545.1890322/1‑94 (MQ=60)
  GGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGct                                                                                              >  SRR4302545.43328/1‑92 (MQ=60)
  GGGCAACAAAACCTGTTGAAATTATTGCTTTTGCAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGct                                                                                              <  SRR4302545.8498470/94‑3 (MQ=60)
   GGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGctg                                                                                             <  SRR4302545.672403/94‑4 (MQ=60)
   GGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGctg                                                                                             >  SRR4302545.16982/1‑91 (MQ=60)
   GGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGctg                                                                                             <  SRR4302545.2616986/94‑4 (MQ=60)
    GCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGG                                                                                            <  SRR4302545.3310997/94‑1 (MQ=60)
    GCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGG                                                                                            >  SRR4302545.7863896/1‑94 (MQ=60)
    GCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGG                                                                                            <  SRR4302545.7903164/94‑1 (MQ=60)
      AACAAAACGTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTC                                                                                          <  SRR4302545.4833584/94‑1 (MQ=60)
       ACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCG                                                                                         >  SRR4302545.847621/1‑94 (MQ=60)
         AAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCG                                                                                       <  SRR4302545.2889417/94‑1 (MQ=60)
         AAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCG                                                                                       >  SRR4302545.10598350/1‑94 (MQ=60)
         AAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCG                                                                                       <  SRR4302545.2439145/94‑1 (MQ=60)
          AAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGT                                                                                      >  SRR4302545.1772931/1‑94 (MQ=60)
          AAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGT                                                                                      >  SRR4302545.4564500/1‑94 (MQ=60)
           AACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTC                                                                                     >  SRR4302545.2057471/1‑94 (MQ=60)
           AACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTC                                                                                     >  SRR4302545.1314202/1‑94 (MQ=60)
           AACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTC                                                                                     >  SRR4302545.9129397/1‑94 (MQ=60)
           AACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTC                                                                                     >  SRR4302545.4147844/1‑94 (MQ=60)
           AACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTC                                                                                     <  SRR4302545.6990169/94‑1 (MQ=60)
           AACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTC                                                                                     >  SRR4302545.2667767/1‑94 (MQ=60)
           AACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTC                                                                                     >  SRR4302545.3240906/1‑94 (MQ=60)
             CCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTT                                                                                   >  SRR4302545.2421681/1‑94 (MQ=60)
             CCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTT                                                                                   >  SRR4302545.10840135/1‑94 (MQ=60)
             CCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTT                                                                                   >  SRR4302545.7360172/1‑94 (MQ=60)
             CCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTT                                                                                   >  SRR4302545.7360173/1‑94 (MQ=60)
              CTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTA                                                                                  >  SRR4302545.5255236/1‑94 (MQ=60)
              CTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTA                                                                                  >  SRR4302545.3927124/1‑94 (MQ=60)
               TGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTAT                                                                                 >  SRR4302545.1692909/1‑94 (MQ=60)
               TGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTAT                                                                                 >  SRR4302545.2668273/1‑94 (MQ=60)
                GTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATC                                                                                >  SRR4302545.9315841/1‑94 (MQ=60)
                GTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATC                                                                                <  SRR4302545.1363821/94‑1 (MQ=60)
                GTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATC                                                                                >  SRR4302545.5194144/1‑94 (MQ=60)
                  TGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAG                                                                              >  SRR4302545.819526/1‑94 (MQ=60)
                  TGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAG                                                                              >  SRR4302545.6428746/1‑94 (MQ=60)
                  TGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAG                                                                              >  SRR4302545.5796242/1‑94 (MQ=60)
                   GAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGG                                                                             <  SRR4302545.6580092/94‑1 (MQ=60)
                   GAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGG                                                                             <  SRR4302545.9259897/94‑1 (MQ=60)
                   GAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCATGATGCGGCGCTGGTCGCGTCTTATCAGG                                                                             <  SRR4302545.9259892/94‑1 (MQ=60)
                    AAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGC                                                                            <  SRR4302545.803093/94‑1 (MQ=60)
                    AAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGC                                                                            <  SRR4302545.6807181/94‑1 (MQ=60)
                      ATTCTTGCTTTTGTAGGCCGGATAAGGCGTTTACGTCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGcccc                                                                          >  SRR4302545.2153775/1‑90 (MQ=60)
                      ATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCT                                                                          >  SRR4302545.2525122/1‑94 (MQ=60)
                      ATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCT                                                                          <  SRR4302545.6809289/94‑1 (MQ=60)
                      ATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCT                                                                          >  SRR4302545.9393675/1‑94 (MQ=60)
                      ATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCT                                                                          <  SRR4302545.9074112/94‑1 (MQ=60)
                       TTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTC                                                                         >  SRR4302545.8361571/1‑94 (MQ=60)
                       TTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTC                                                                         >  SRR4302545.8361567/1‑94 (MQ=60)
                       TTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTA                                                                         <  SRR4302545.7431741/94‑1 (MQ=60)
                       TTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTA                                                                         >  SRR4302545.6350838/1‑94 (MQ=60)
                        TATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCTTGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTAC                                                                        <  SRR4302545.7584914/94‑1 (MQ=60)
                        TATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTAC                                                                        >  SRR4302545.4695304/1‑94 (MQ=60)
                        TATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTAC                                                                        >  SRR4302545.1911426/1‑94 (MQ=60)
                         ATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACG                                                                       >  SRR4302545.2982747/1‑94 (MQ=60)
                         ATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCCCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACG                                                                       >  SRR4302545.7981951/1‑94 (MQ=60)
                          TTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGA                                                                      >  SRR4302545.3763413/1‑94 (MQ=60)
                            GCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACT                                                                    <  SRR4302545.9433205/94‑1 (MQ=60)
                             CTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTT                                                                   >  SRR4302545.1278973/1‑94 (MQ=60)
                             CTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTT                                                                   >  SRR4302545.3540741/1‑94 (MQ=60)
                              TTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTGCGACTTT                                                                  >  SRR4302545.992799/1‑94 (MQ=60)
                              TTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTT                                                                  >  SRR4302545.4575842/1‑94 (MQ=60)
                              TTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTT                                                                  >  SRR4302545.9807112/1‑94 (MQ=60)
                               tttgtggccgGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTC                                                                 <  SRR4302545.3959914/84‑1 (MQ=60)
                                TTGTAGGCCGGATAAGGCTTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCT                                                                <  SRR4302545.6206387/94‑1 (MQ=60)
                                TTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTCAGACTTTCT                                                                >  SRR4302545.9808198/1‑94 (MQ=60)
                                TTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCT                                                                <  SRR4302545.5856023/94‑1 (MQ=60)
                                TTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCT                                                                >  SRR4302545.4183008/1‑94 (MQ=60)
                                TTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCT                                                                >  SRR4302545.4232106/1‑94 (MQ=60)
                                 TGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTC                                                               >  SRR4302545.10568350/1‑94 (MQ=60)
                                   TAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCG                                                             >  SRR4302545.4645358/1‑94 (MQ=60)
                                   TAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCG                                                             >  SRR4302545.8385327/1‑94 (MQ=60)
                                     GGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCA                                                           <  SRR4302545.7510085/94‑1 (MQ=60)
                                     GGCCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCA                                                           <  SRR4302545.5558083/94‑1 (MQ=60)
                                       CCGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATC                                                         >  SRR4302545.7654191/1‑94 (MQ=60)
                                        CGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCC                                                        >  SRR4302545.509297/1‑94 (MQ=60)
                                        CGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCC                                                        >  SRR4302545.319547/1‑94 (MQ=60)
                                        CGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCC                                                        >  SRR4302545.6732760/1‑94 (MQ=60)
                                        CGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCC                                                        <  SRR4302545.7263232/94‑1 (MQ=60)
                                        CGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCC                                                        >  SRR4302545.1594705/1‑94 (MQ=60)
                                        CGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCC                                                        <  SRR4302545.7263230/94‑1 (MQ=60)
                                        CGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCC                                                        <  SRR4302545.2098408/94‑1 (MQ=60)
                                        CGGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCC                                                        >  SRR4302545.8004661/1‑94 (MQ=60)
                                         GGATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCG                                                       <  SRR4302545.5778763/94‑1 (MQ=60)
                                          GATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGG                                                      <  SRR4302545.6521883/94‑1 (MQ=60)
                                          GATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGG                                                      <  SRR4302545.6521882/94‑1 (MQ=60)
                                          GATAAGGCAGTTACGCCGCAGCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGG                                                      <  SRR4302545.8542459/94‑1 (MQ=60)
                                          GATAAGGAGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGG                                                      <  SRR4302545.7010239/94‑1 (MQ=60)
                                           ATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGC                                                     >  SRR4302545.7682140/1‑94 (MQ=60)
                                           ATAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGC                                                     >  SRR4302545.427026/1‑94 (MQ=60)
                                            TAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCA                                                    >  SRR4302545.5992322/1‑94 (MQ=60)
                                            TAAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCA                                                    >  SRR4302545.5992323/1‑94 (MQ=60)
                                             AAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCAT                                                   >  SRR4302545.3073815/1‑94 (MQ=60)
                                             AAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCAT                                                   <  SRR4302545.919644/94‑1 (MQ=60)
                                             AAGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCAT                                                   >  SRR4302545.1914285/1‑94 (MQ=60)
                                              AGGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATA                                                  >  SRR4302545.666327/1‑94 (MQ=60)
                                               GGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCCTAA                                                 >  SRR4302545.4941918/1‑94 (MQ=60)
                                               GGCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAA                                                 >  SRR4302545.9453748/1‑94 (MQ=60)
                                               GGCGTTTACGACGCATCAGG‑CA‑TCTGAGCATGATGCCCGATGCGGCGCTGGTCGCATCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAA                                                 >  SRR4302545.1772873/1‑94 (MQ=60)
                                                GCGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAA                                                >  SRR4302545.7250018/1‑94 (MQ=60)
                                                 CGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAAC                                               >  SRR4302545.1613339/1‑94 (MQ=60)
                                                 CGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAAC                                               >  SRR4302545.8320343/1‑94 (MQ=60)
                                                 CGTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAAC                                               <  SRR4302545.10658978/94‑1 (MQ=60)
                                                  GTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATACACA                                              >  SRR4302545.4197388/1‑94 (MQ=60)
                                                  GTTTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACA                                              >  SRR4302545.7356886/1‑94 (MQ=60)
                                                    TTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAAC                                            <  SRR4302545.6484721/94‑1 (MQ=60)
                                                    TTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAAC                                            >  SRR4302545.10861929/1‑94 (MQ=60)
                                                    TTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAAC                                            <  SRR4302545.2833311/94‑1 (MQ=60)
                                                    TTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAAC                                            >  SRR4302545.352775/1‑94 (MQ=60)
                                                    TTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAAC                                            <  SRR4302545.8768732/94‑1 (MQ=60)
                                                    TTACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAAC                                            >  SRR4302545.10640739/1‑94 (MQ=60)
                                                     TACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACG                                           <  SRR4302545.5740175/94‑1 (MQ=60)
                                                     TACGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACG                                           <  SRR4302545.5492687/94‑1 (MQ=60)
                                                       CGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAG                                         <  SRR4302545.1453844/94‑1 (MQ=60)
                                                       CGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAG                                         <  SRR4302545.9596139/94‑1 (MQ=60)
                                                       CGCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAG                                         >  SRR4302545.9135964/1‑94 (MQ=60)
                                                        GCCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGCGC                                        >  SRR4302545.7659009/1‑94 (MQ=60)
                                                        GCCGCATCAGG‑‑ATTCTGAGCATGATGACTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTAACTCAGCATCCGGCAAAAACAACGAGC                                        <  SRR4302545.9651988/94‑1 (MQ=45)
                                                         CCGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCT                                       <  SRR4302545.9595396/94‑1 (MQ=60)
                                                          CGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTT                                      >  SRR4302545.3933341/1‑94 (MQ=60)
                                                          CGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTT                                      <  SRR4302545.1092064/94‑1 (MQ=60)
                                                          CGCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTT                                      >  SRR4302545.5442877/1‑94 (MQ=60)
                                                           GCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTC                                     >  SRR4302545.10324418/1‑94 (MQ=60)
                                                           GCATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTC                                     <  SRR4302545.3395770/94‑1 (MQ=60)
                                                            CATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCA                                    >  SRR4302545.10161055/1‑94 (MQ=60)
                                                            CATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCA                                    <  SRR4302545.4654977/94‑1 (MQ=60)
                                                             ATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAG                                   >  SRR4302545.2514603/1‑94 (MQ=60)
                                                             ATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAG                                   <  SRR4302545.4315118/94‑1 (MQ=60)
                                                             ATCAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAG                                   >  SRR4302545.2049382/1‑94 (MQ=60)
                                                               CAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCT                                 <  SRR4302545.10784912/94‑1 (MQ=60)
                                                               CAGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCT                                 >  SRR4302545.7258205/1‑94 (MQ=60)
                                                                AGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATGCTGCATAAACAACGAGCTTCAGCTA                                >  SRR4302545.6154210/1‑94 (MQ=60)
                                                                AGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTA                                <  SRR4302545.6325829/94‑1 (MQ=60)
                                                                AGG‑CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTA                                >  SRR4302545.10714488/1‑94 (MQ=60)
                                                                  GGCA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAA                               <  SRR4302545.3674569/94‑1 (MQ=60)
                                                                   GCA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAA                              >  SRR4302545.7872105/1‑94 (MQ=60)
                                                                   GCA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAA                              <  SRR4302545.3087625/94‑1 (MQ=60)
                                                                   GCA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAA                              >  SRR4302545.7182054/1‑94 (MQ=60)
                                                                    CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAAC                             >  SRR4302545.8883842/1‑94 (MQ=60)
                                                                    CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAAC                             <  SRR4302545.7041382/94‑1 (MQ=60)
                                                                    CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAAC                             >  SRR4302545.1534310/1‑94 (MQ=60)
                                                                    CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAAC                             <  SRR4302545.1072075/94‑1 (MQ=60)
                                                                    CA‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGACTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAAC                             >  SRR4302545.908514/1‑94 (MQ=60)
                                                                     A‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTcaacc                            >  SRR4302545.10381379/1‑89 (MQ=60)
                                                                     A‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACA                            >  SRR4302545.8603017/1‑94 (MQ=60)
                                                                     A‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACA                            <  SRR4302545.1163649/94‑1 (MQ=60)
                                                                     A‑TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACA                            >  SRR4302545.7516579/1‑94 (MQ=60)
                                                                       TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAG                           <  SRR4302545.10071723/94‑1 (MQ=60)
                                                                       TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAG                           <  SRR4302545.1793714/94‑1 (MQ=60)
                                                                       TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAG                           <  SRR4302545.5225587/94‑1 (MQ=60)
                                                                        CTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGC                          <  SRR4302545.1576119/94‑1 (MQ=60)
                                                                          GAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCC                        <  SRR4302545.5070018/94‑1 (MQ=60)
                                                                          GAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCC                        <  SRR4302545.3626083/94‑1 (MQ=60)
                                                                          GAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCC                        >  SRR4302545.10841231/1‑94 (MQ=60)
                                                                          GAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCC                        >  SRR4302545.2652895/1‑94 (MQ=60)
                                                                           AGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCA                       <  SRR4302545.3772717/94‑1 (MQ=60)
                                                                           AGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCA                       >  SRR4302545.4558426/1‑94 (MQ=60)
                                                                           AGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCA                       >  SRR4302545.6089314/1‑94 (MQ=60)
                                                                            GCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAA                      <  SRR4302545.321789/94‑1 (MQ=60)
                                                                             CATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAAT                     <  SRR4302545.1292206/94‑1 (MQ=60)
                                                                             CATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAAT                     <  SRR4302545.4590785/94‑1 (MQ=60)
                                                                              ATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATC                    <  SRR4302545.884555/94‑1 (MQ=60)
                                                                              ATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATC                    >  SRR4302545.1989390/1‑94 (MQ=60)
                                                                              ATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATC                    >  SRR4302545.10416796/1‑94 (MQ=60)
                                                                              ATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATC                    <  SRR4302545.5300592/94‑1 (MQ=60)
                                                                               TGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCA                   <  SRR4302545.10370679/94‑1 (MQ=60)
                                                                               TGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCA                   >  SRR4302545.5320318/1‑94 (MQ=60)
                                                                                GATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCAT                  <  SRR4302545.36762/94‑1 (MQ=60)
                                                                                GATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCAT                  <  SRR4302545.3000281/94‑1 (MQ=60)
                                                                                GATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCAT                  >  SRR4302545.3983568/1‑94 (MQ=60)
                                                                                GATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCAT                  <  SRR4302545.7413630/94‑1 (MQ=60)
                                                                                GATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGACCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCAT                  <  SRR4302545.10202192/94‑1 (MQ=60)
                                                                                 ATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATT                 <  SRR4302545.8399610/94‑1 (MQ=60)
                                                                                 ATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATT                 >  SRR4302545.8279729/1‑94 (MQ=60)
                                                                                 ATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATT                 >  SRR4302545.2233816/1‑94 (MQ=60)
                                                                                 ATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATT                 <  SRR4302545.10749292/94‑1 (MQ=60)
                                                                                  TGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTG                >  SRR4302545.5897615/1‑94 (MQ=60)
                                                                                   GCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGC               >  SRR4302545.3505765/1‑94 (MQ=60)
                                                                                    CCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCG              >  SRR4302545.9623908/1‑94 (MQ=60)
                                                                                    CCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCG              >  SRR4302545.644073/1‑94 (MQ=60)
                                                                                    CCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCG              >  SRR4302545.2442585/1‑94 (MQ=60)
                                                                                    CCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCG              >  SRR4302545.3511086/1‑94 (MQ=60)
                                                                                     CTGATGCGGCGCTGGTCGCGTCTTATCAGGTCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGG             <  SRR4302545.10652236/94‑1 (MQ=60)
                                                                                     CTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTTAGCTAAACAGCCCAATCATTGCGG             >  SRR4302545.7803456/1‑94 (MQ=60)
                                                                                      TGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGC            <  SRR4302545.9518779/94‑1 (MQ=60)
                                                                                        ATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCAC          <  SRR4302545.5684197/94‑1 (MQ=60)
                                                                                        ATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGATTCAGCTAAACAGCCCAATCATTGCGGCAC          >  SRR4302545.5226529/1‑94 (MQ=60)
                                                                                         TGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACA         >  SRR4302545.8603736/1‑94 (MQ=60)
                                                                                         TGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACA         >  SRR4302545.2827145/1‑94 (MQ=60)
                                                                                         TGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACA         >  SRR4302545.9731314/1‑94 (MQ=60)
                                                                                         TGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACA         >  SRR4302545.3430345/1‑94 (MQ=60)
                                                                                           CGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACA       <  SRR4302545.7132560/94‑1 (MQ=60)
                                                                                           CGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACA       >  SRR4302545.7600560/1‑94 (MQ=60)
                                                                                           CGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACA       >  SRR4302545.4202165/1‑94 (MQ=60)
                                                                                            GGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAG      >  SRR4302545.3460203/1‑94 (MQ=60)
                                                                                            GGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAG      >  SRR4302545.4150713/1‑94 (MQ=60)
                                                                                             GCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAGC     >  SRR4302545.809649/1‑94 (MQ=60)
                                                                                               C‑TGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAGCACG  <  SRR4302545.4309003/94‑1 (MQ=60)
                                                                                               C‑TGGTCGCGGCTTATCAGGCCTACGACTTTCTCCTCATTCTGCATAAACAACGACCTTCAGCTAAACAGCCCAATCATCGCGGCACACAGCACG  >  SRR4302545.8931070/1‑94 (MQ=60)
                                                                                               |                                                                                              
CTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCCA‑TCTGAGCATGATGCCTGATGCGGCG‑TGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAGCACG  >  NC_000913/4051076‑4051263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑