Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,080,993 T→C T804A (ACT→GCT)  fdoG ← formate dehydrogenase‑O, major subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,080,9930TC98.9% 1594.3 / 2.6 468T804A (ACT→GCT) fdoGformate dehydrogenase‑O, major subunit
Reads supporting (aligned to +/- strand):  ref base T (4/1);  new base C (266/197);  total (270/198)
Fisher's exact test for biased strand distribution p-value = 4.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01

Only 100 of 493 total aligned reads displayed.

CCCATGCCTTCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAG  >  NC_000913/4080902‑4081083
                                                                                           |                                                                                          
cccATGCCTTCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAgcgc                                                                                          >  2:6353035/1‑94 (MQ=255)
cccATGCCTTCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGAGCTGTAGTCCGCAATATCCCAGCCGGTCCACTTAgcgc                                                                                          >  1:10598605/1‑94 (MQ=255)
 ccATGCCTTCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGcc                                                                                         <  1:610064/94‑1 (MQ=255)
 ccATGCCTTCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGcc                                                                                         >  1:8946552/1‑94 (MQ=255)
  cATGCCTTCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCg                                                                                        >  2:9611180/1‑94 (MQ=255)
    tGCCTTCCTGCTGCATGATAAACGGCCCGACGCTGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTc                                                                                      >  2:683933/1‑94 (MQ=255)
      ccTTGCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTccc                                                                                    >  1:10369882/1‑94 (MQ=255)
       cTTCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCa                                                                                   <  2:20381/94‑1 (MQ=255)
        ttCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCAt                                                                                  <  1:4923477/94‑1 (MQ=255)
        ttCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCAt                                                                                  >  1:9092191/1‑94 (MQ=255)
           ctgctgCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTc                                                                               >  2:9088035/1‑94 (MQ=255)
           ctgctgCATGAGAGAGGGCCCGACGCCCCTACCCGGAGGCGCTGCGGTGTAGTCCGGAATACCCCAGCCGGTCCACTTAGCGCCGTCCCATTTc                                                                               <  2:6037783/94‑1 (MQ=255)
            tgctgcATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCa                                                                              >  1:5526493/1‑94 (MQ=255)
               tgcATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCa                                                                           >  1:10039834/1‑94 (MQ=255)
               tgcATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCa                                                                           >  1:5704169/1‑94 (MQ=255)
               tgcATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCa                                                                           >  2:7154025/1‑94 (MQ=255)
                gcATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCCTTTCAGCaa                                                                          >  2:737126/1‑94 (MQ=255)
                 cATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAAc                                                                         <  1:654116/94‑1 (MQ=255)
                  aTGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACt                                                                        <  2:1684997/94‑1 (MQ=255)
                   cgATAAACGGCCCGACGCCTGTTCCGGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTg                                                                       <  2:1201279/93‑1 (MQ=255)
                     aTAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCCTTTCAGCAACTGAc                                                                     >  2:2866731/1‑94 (MQ=255)
                      tAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACg                                                                    >  2:2646330/1‑94 (MQ=255)
                       aaaCGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAACGCCGTCCCATTTCAGAAACTGACGc                                                                   >  2:371510/1‑94 (MQ=255)
                        aaCGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCt                                                                  <  1:10179291/94‑1 (MQ=255)
                        aaCGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCt                                                                  >  1:455013/1‑94 (MQ=255)
                         aCGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCtt                                                                 >  2:4410921/1‑94 (MQ=255)
                         aCGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCtt                                                                 <  2:4741754/94‑1 (MQ=255)
                          cGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTc                                                                >  2:6197050/1‑94 (MQ=255)
                          cGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTc                                                                >  1:5978174/1‑94 (MQ=255)
                          cGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTc                                                                <  1:9978146/94‑1 (MQ=255)
                          cGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTc                                                                >  2:9758137/1‑94 (MQ=255)
                          cGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTc                                                                <  1:693878/94‑1 (MQ=255)
                             cccGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGa                                                             <  1:8434371/94‑1 (MQ=255)
                              ccGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGAt                                                            >  2:5236155/1‑94 (MQ=255)
                              ccGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGAt                                                            >  1:8376459/1‑94 (MQ=255)
                                gACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATcc                                                          >  2:8641617/1‑94 (MQ=255)
                                gACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATcc                                                          <  2:2476120/94‑1 (MQ=255)
                                  cgccgcTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCa                                                        <  1:2868660/94‑1 (MQ=255)
                                   gccgcTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCAc                                                       >  1:938117/1‑94 (MQ=255)
                                   gccgcTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCAc                                                       >  1:3953700/1‑94 (MQ=255)
                                    ccgcTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACg                                                      <  1:1146864/94‑1 (MQ=255)
                                     cgcTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACgg                                                     >  1:2482678/1‑94 (MQ=255)
                                     cgcTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACgg                                                     >  1:5281393/1‑94 (MQ=255)
                                     cgcTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCAACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACgg                                                     >  1:953612/1‑94 (MQ=255)
                                      ccTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACggg                                                    <  2:9903037/93‑1 (MQ=255)
                                        tACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGtt                                                  <  2:7546243/94‑1 (MQ=255)
                                            cGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTAcgc                                              >  1:2189939/1‑92 (MQ=255)
                                             ggAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCt                                             >  2:6602011/1‑94 (MQ=255)
                                             ggAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCt                                             >  2:3987673/1‑94 (MQ=255)
                                             ggAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCt                                             <  2:1817108/94‑1 (MQ=255)
                                              gAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTg                                            <  2:3178682/94‑1 (MQ=255)
                                              gAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTg                                            <  2:824500/94‑1 (MQ=255)
                                                ggCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCg                                          <  1:8009215/94‑1 (MQ=255)
                                                 gcgctgcgctGTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCgg                                         <  2:4959388/94‑1 (MQ=255)
                                                  ggctccgctgTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGa                                        <  2:10335482/93‑1 (MQ=255)
                                                  cgctgcgctgTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGa                                        >  1:2355052/1‑94 (MQ=255)
                                                     tgcgctgTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCt                                     >  1:7239287/1‑94 (MQ=255)
                                                     tgcgctgTAGTCCGGAATACCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCt                                     <  2:10571512/94‑1 (MQ=255)
                                                      gcgctgTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTg                                    >  1:2740764/1‑94 (MQ=255)
                                                      gcgctgTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTg                                    >  1:409005/1‑94 (MQ=255)
                                                        gctgTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCg                                  <  1:683933/94‑1 (MQ=255)
                                                        gctgTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCg                                  >  1:467395/1‑94 (MQ=255)
                                                        gctgTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCg                                  <  2:7778176/94‑1 (MQ=255)
                                                         ctgTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTTCCCTGCGGATCTGCgg                                 <  1:568983/94‑1 (MQ=255)
                                                         ctgTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCgg                                 <  1:3222501/94‑1 (MQ=255)
                                                          tgTAGTCCGGACTATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCCGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGa                                >  1:5819446/1‑94 (MQ=255)
                                                          tgTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGa                                >  2:7313293/1‑94 (MQ=255)
                                                          tgTAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGa                                >  1:3425650/1‑94 (MQ=255)
                                                           ctAGTCGGTAATACCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAg                               <  1:4824810/93‑1 (MQ=255)
                                                            tAGTCCGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAgg                              >  1:4250457/1‑94 (MQ=255)
                                                                ccGGAATCTCCCAGCCGGTCCACTTAGCGCCCTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGcgcg                          <  2:2109344/94‑1 (MQ=255)
                                                                ccGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGCCGCTTCGGATCCCCCGGGTTACCCTGCGGATCTGCGGAGGcgcg                          >  1:2081972/1‑94 (MQ=255)
                                                                ccGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGcgcg                          >  2:893108/1‑94 (MQ=255)
                                                                ccGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGcgcg                          >  2:5422114/1‑94 (MQ=255)
                                                                ccGGAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGcgcg                          <  2:139447/94‑1 (MQ=255)
                                                                  ggAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGt                        >  1:3719794/1‑94 (MQ=255)
                                                                   gAATATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGtt                       >  1:2624560/1‑94 (MQ=255)
                                                                    aaTATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCTCGGTta                      >  1:8879754/1‑94 (MQ=255)
                                                                    aaTATCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTta                      >  1:9690307/1‑94 (MQ=255)
                                                                     atatCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTtat                     >  1:7403167/1‑94 (MQ=255)
                                                                      tatCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTtata                    <  2:505940/94‑1 (MQ=255)
                                                                       atCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCTCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGGTATAc                   >  1:6448448/1‑94 (MQ=255)
                                                                       atCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATAc                   >  2:4307369/1‑94 (MQ=255)
                                                                       atCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATAc                   <  1:1744542/94‑1 (MQ=255)
                                                                        tCCCAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACa                  >  1:3065525/1‑94 (MQ=255)
                                                                        tCCCAGCCGGTCCACGTAGCGCCCTACCATGTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACa                  <  2:7694077/94‑1 (MQ=255)
                                                                         cccAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGCGGCGGGTATACaa                 >  1:7154617/1‑93 (MQ=255)
                                                                          ccAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGa                >  2:5860743/1‑94 (MQ=255)
                                                                          ccAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGa                >  1:776312/1‑94 (MQ=255)
                                                                          ccAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATATGCGGAGGCGCGGTTATACAGa                >  1:520350/1‑94 (MQ=255)
                                                                           cAGCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGaa               >  1:6718403/1‑94 (MQ=255)
                                                                             gCCGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATg             <  1:1451814/94‑1 (MQ=255)
                                                                               cGGTCCGCTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATgcg           <  2:8399603/94‑1 (MQ=255)
                                                                               cGGTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATgcg           >  1:8248484/1‑94 (MQ=255)
                                                                                ggTCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATgcgg          >  2:942809/1‑94 (MQ=255)
                                                                                  tCCACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATgcggcg        >  2:223821/1‑94 (MQ=255)
                                                                                   ccACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATGcggcgg       >  2:8028999/1‑94 (MQ=255)
                                                                                    cACTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGt      >  1:8698316/1‑94 (MQ=255)
                                                                                      cTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTa    <  1:6293743/94‑1 (MQ=255)
                                                                                      cTTAGCGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTa    >  2:3385619/1‑94 (MQ=255)
                                                                                           |                                                                                          
CCCATGCCTTCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAG  >  NC_000913/4080902‑4081083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 324 total aligned reads displayed.
GGAGAGCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACC  >  NC_000913/4082923‑4083109
                                                                                             |                                                                                             
GGAGAGCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAAT                                                                                               >  SRR4302545.1760110/1‑94 (MQ=60)
GGAGAGCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAAT                                                                                               <  SRR4302545.7060584/94‑1 (MQ=60)
GGAGAGCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAAT                                                                                               <  SRR4302545.5927210/94‑1 (MQ=60)
 GAGAGCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATG                                                                                              <  SRR4302545.2229403/94‑1 (MQ=60)
 GAGAGCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATG                                                                                              <  SRR4302545.3135391/94‑1 (MQ=60)
 GAGAGCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATG                                                                                              <  SRR4302545.8164793/94‑1 (MQ=60)
 GAGAGCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATG                                                                                              >  SRR4302545.520842/1‑94 (MQ=60)
 GAGAGCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATG                                                                                              <  SRR4302545.10452637/94‑1 (MQ=60)
  AGAGCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGC                                                                                             <  SRR4302545.10298249/94‑1 (MQ=60)
  AGAGCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGC                                                                                             >  SRR4302545.10304008/1‑94 (MQ=60)
   GAGCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTTGCGATGCGATCAAACGCC                                                                                            <  SRR4302545.4462808/94‑1 (MQ=60)
   GAGCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCC                                                                                            <  SRR4302545.8891625/94‑1 (MQ=60)
   GAGCCAGCGGTTAAAAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCC                                                                                            <  SRR4302545.8891627/94‑1 (MQ=60)
    AGCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCT                                                                                           <  SRR4302545.6845118/94‑1 (MQ=60)
      CCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCT                                                                                         <  SRR4302545.3429544/94‑1 (MQ=60)
      CCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGACTCT                                                                                         >  SRR4302545.9812072/1‑94 (MQ=60)
       CAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTT                                                                                        >  SRR4302545.2868859/1‑94 (MQ=60)
       CAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTT                                                                                        <  SRR4302545.6768559/94‑1 (MQ=60)
       CAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTT                                                                                        <  SRR4302545.2532297/94‑1 (MQ=60)
         GCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCC                                                                                      >  SRR4302545.643538/1‑94 (MQ=60)
         GCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCC                                                                                      <  SRR4302545.9369716/94‑1 (MQ=60)
          CGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCC                                                                                     <  SRR4302545.103057/94‑1 (MQ=60)
          CGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCC                                                                                     >  SRR4302545.5669203/1‑94 (MQ=60)
          CGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCC                                                                                     >  SRR4302545.6229934/1‑94 (MQ=60)
           GGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCTATGCGATCAAATGCCTCTTCCCA                                                                                    >  SRR4302545.6007733/1‑94 (MQ=60)
           GGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCA                                                                                    >  SRR4302545.4981662/1‑94 (MQ=60)
            GTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAA                                                                                   >  SRR4302545.4115024/1‑94 (MQ=60)
             TTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAAC                                                                                  >  SRR4302545.429753/1‑94 (MQ=60)
             TTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAAC                                                                                  <  SRR4302545.197834/94‑1 (MQ=60)
              TAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACT                                                                                 <  SRR4302545.8598640/94‑1 (MQ=60)
              TAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACT                                                                                 >  SRR4302545.2007100/1‑94 (MQ=60)
              TAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACT                                                                                 >  SRR4302545.8932414/1‑94 (MQ=60)
              TAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATAGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACT                                                                                 >  SRR4302545.5717397/1‑94 (MQ=60)
               AACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTG                                                                                >  SRR4302545.10091931/1‑94 (MQ=60)
               AACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTG                                                                                >  SRR4302545.2432012/1‑94 (MQ=60)
               AACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTG                                                                                >  SRR4302545.10911912/1‑94 (MQ=60)
                ACAGTCACGCCTTCGGCGTTTTGCGCAATGCAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGA                                                                               >  SRR4302545.9069561/1‑94 (MQ=60)
                 CAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGCT                                                                              <  SRR4302545.6649111/94‑1 (MQ=60)
                 CAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGAT                                                                              >  SRR4302545.6901093/1‑94 (MQ=60)
                 CAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGAT                                                                              >  SRR4302545.3738974/1‑94 (MQ=60)
                 CAGACACGCCTTCGGCGTTTTGCGCAATTTAGTTAGCATCGCGGACTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCACCTGAT                                                                              >  SRR4302545.972864/1‑94 (MQ=60)
                  AGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATTCCTCTTCCCAACTGATT                                                                             <  SRR4302545.4952879/94‑1 (MQ=60)
                  AGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATT                                                                             <  SRR4302545.7398106/94‑1 (MQ=60)
                    TCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTG                                                                           <  SRR4302545.286231/94‑1 (MQ=60)
                     CACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGC                                                                          <  SRR4302545.7276373/94‑1 (MQ=60)
                     CACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGC                                                                          >  SRR4302545.10644634/1‑94 (MQ=60)
                     CACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGC                                                                          >  SRR4302545.9424395/1‑94 (MQ=60)
                     CACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGC                                                                          >  SRR4302545.322295/1‑94 (MQ=60)
                     CACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGACTCTTCCCAACTGATTTGC                                                                          >  SRR4302545.2440802/1‑94 (MQ=60)
                      ACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCT                                                                         <  SRR4302545.7923197/94‑1 (MQ=60)
                      ACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCT                                                                         >  SRR4302545.10182605/1‑94 (MQ=60)
                       CGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTG                                                                        <  SRR4302545.1677489/94‑1 (MQ=60)
                       CGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTG                                                                        <  SRR4302545.3278729/94‑1 (MQ=60)
                       CGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTA                                                                        <  SRR4302545.7411252/94‑1 (MQ=60)
                        GCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGTCTCTTCCCAACTGATTTGCTGC                                                                       >  SRR4302545.5428740/1‑94 (MQ=60)
                        GCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGC                                                                       <  SRR4302545.4495151/94‑1 (MQ=60)
                          CTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCA                                                                     >  SRR4302545.698880/1‑94 (MQ=60)
                          CTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCA                                                                     >  SRR4302545.2790814/1‑94 (MQ=60)
                           TTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCAT                                                                    >  SRR4302545.9023071/1‑94 (MQ=60)
                            TCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATT                                                                   <  SRR4302545.4035031/94‑1 (MQ=60)
                             CGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTT                                                                  >  SRR4302545.1582910/1‑94 (MQ=60)
                             CGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTT                                                                  <  SRR4302545.4564549/94‑1 (MQ=60)
                             CGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCATCCCAACTGATTTGCTGCCATTT                                                                  >  SRR4302545.7790793/1‑94 (MQ=60)
                              GGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTA                                                                 >  SRR4302545.2617147/1‑94 (MQ=60)
                              GGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTA                                                                 >  SRR4302545.3754932/1‑94 (MQ=60)
                               GCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTAT                                                                <  SRR4302545.249723/94‑1 (MQ=60)
                               GCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTAT                                                                <  SRR4302545.9109761/94‑1 (MQ=60)
                               GCGTTTAGCGCGATGAAGTTAGCATCGCGGTCTTCTTTCATCAGTTTAGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATATAT                                                                <  SRR4302545.9688415/94‑1 (MQ=60)
                                CGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATC                                                               <  SRR4302545.8394804/94‑1 (MQ=60)
                                CGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATC                                                               >  SRR4302545.42296/1‑94 (MQ=60)
                                 GTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCA                                                              <  SRR4302545.99078/94‑1 (MQ=60)
                                 GTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCA                                                              >  SRR4302545.8583563/1‑94 (MQ=60)
                                 GTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCA                                                              >  SRR4302545.7386846/1‑94 (MQ=60)
                                  TTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAG                                                             <  SRR4302545.7674171/94‑1 (MQ=60)
                                  TTTTGCGAAATGTAGTTAGCATCGCGTTCTTCTTTCATCAGTTTGGCGATGCGATAAAATCCCTCTTCCCAACTGATgaaagaagaccgcgatg                                                             <  SRR4302545.7928980/94‑18 (MQ=60)
                                   TTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGA                                                            <  SRR4302545.8751193/94‑1 (MQ=60)
                                    TTGCGCAATGTATTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAC                                                           >  SRR4302545.5831055/1‑94 (MQ=60)
                                    TTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAA                                                           >  SRR4302545.4677023/1‑94 (MQ=60)
                                     TGCGCAATGTAGTTAGCATTGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAAC                                                          <  SRR4302545.3180331/94‑1 (MQ=60)
                                     TGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAAC                                                          <  SRR4302545.2360672/94‑1 (MQ=60)
                                     TGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAAC                                                          <  SRR4302545.8499966/94‑1 (MQ=60)
                                     TGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAAC                                                          >  SRR4302545.9736451/1‑94 (MQ=60)
                                     TGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAAC                                                          <  SRR4302545.4505237/94‑1 (MQ=60)
                                      GCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACC                                                         <  SRR4302545.132270/94‑1 (MQ=60)
                                      GCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACC                                                         >  SRR4302545.5422617/1‑94 (MQ=60)
                                      GCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACC                                                         <  SRR4302545.8846192/94‑1 (MQ=60)
                                      GCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACC                                                         <  SRR4302545.4856140/94‑1 (MQ=60)
                                       CGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCT                                                        <  SRR4302545.8508215/94‑1 (MQ=60)
                                       CGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCT                                                        <  SRR4302545.8034256/94‑1 (MQ=60)
                                       CGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCT                                                        <  SRR4302545.10916714/94‑1 (MQ=60)
                                        GCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAGATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTG                                                       <  SRR4302545.1225621/94‑1 (MQ=60)
                                        GCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTG                                                       <  SRR4302545.7860595/94‑1 (MQ=60)
                                        GCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTG                                                       <  SRR4302545.10742463/94‑1 (MQ=60)
                                        GCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTG                                                       <  SRR4302545.7128687/94‑1 (MQ=60)
                                          AATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGC                                                     <  SRR4302545.1118117/94‑1 (MQ=60)
                                          AATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGC                                                     <  SRR4302545.3050256/94‑1 (MQ=60)
                                          AATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGA                                                     >  SRR4302545.7054325/1‑94 (MQ=60)
                                           ATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCG                                                    <  SRR4302545.953199/94‑1 (MQ=60)
                                           ATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCG                                                    <  SRR4302545.5242965/94‑1 (MQ=60)
                                           ATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCG                                                    <  SRR4302545.10028987/94‑1 (MQ=60)
                                           ATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTAGCG                                                    <  SRR4302545.953198/94‑1 (MQ=60)
                                              TAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCAC                                                 >  SRR4302545.8843373/1‑94 (MQ=60)
                                               AGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACG                                                >  SRR4302545.5596809/1‑94 (MQ=60)
                                               AGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACG                                                >  SRR4302545.1224527/1‑94 (MQ=60)
                                               AGTTAGCATCGCGGTCTTCTTTCATCAATTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACG                                                <  SRR4302545.4432009/94‑1 (MQ=60)
                                                GTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGG                                               >  SRR4302545.2589085/1‑94 (MQ=60)
                                                 TTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCATCTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGT                                              >  SRR4302545.5241289/1‑94 (MQ=60)
                                                 TTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGT                                              >  SRR4302545.10394044/1‑94 (MQ=60)
                                                 TTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGT                                              >  SRR4302545.6703542/1‑94 (MQ=60)
                                                 TTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGT                                              <  SRR4302545.4021871/94‑1 (MQ=60)
                                                   AGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTAT                                            >  SRR4302545.5142637/1‑94 (MQ=60)
                                                   AGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTAT                                            >  SRR4302545.10729287/1‑94 (MQ=60)
                                                   AGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTAT                                            <  SRR4302545.10826041/94‑1 (MQ=60)
                                                    GCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATT                                           <  SRR4302545.4059360/94‑1 (MQ=60)
                                                     CATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTC                                          <  SRR4302545.1044425/94‑1 (MQ=60)
                                                      ATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCC                                         >  SRR4302545.8476462/1‑94 (MQ=60)
                                                      ATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCC                                         >  SRR4302545.559572/1‑94 (MQ=60)
                                                      ATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCC                                         >  SRR4302545.4217552/1‑94 (MQ=60)
                                                       TCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCG                                        <  SRR4302545.3523150/94‑1 (MQ=60)
                                                       TCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCG                                        <  SRR4302545.789158/94‑1 (MQ=60)
                                                       TCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCG                                        <  SRR4302545.4084818/94‑1 (MQ=60)
                                                        CGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGG                                       <  SRR4302545.5511229/94‑1 (MQ=60)
                                                          CGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAA                                     <  SRR4302545.1504310/94‑1 (MQ=60)
                                                          CGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAA                                     >  SRR4302545.9904510/1‑94 (MQ=60)
                                                          CGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAA                                     >  SRR4302545.4880404/1‑94 (MQ=60)
                                                           GGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAA                                    >  SRR4302545.8250340/1‑94 (MQ=60)
                                                           GGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAA                                    >  SRR4302545.1125239/1‑94 (MQ=60)
                                                           GGTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAA                                    >  SRR4302545.7637216/1‑94 (MQ=60)
                                                            GTCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAAC                                   >  SRR4302545.2208309/1‑94 (MQ=60)
                                                             TCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACT                                  >  SRR4302545.1511360/1‑94 (MQ=60)
                                                             TCTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACT                                  >  SRR4302545.6465643/1‑94 (MQ=60)
                                                              CTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTT                                 <  SRR4302545.4930406/94‑1 (MQ=60)
                                                              CTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTT                                 <  SRR4302545.3032053/94‑1 (MQ=60)
                                                              CTTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTT                                 >  SRR4302545.3104622/1‑94 (MQ=60)
                                                               TTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTC                                >  SRR4302545.3531566/1‑94 (MQ=60)
                                                               TTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTC                                >  SRR4302545.6092064/1‑94 (MQ=60)
                                                               TTCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTC                                <  SRR4302545.4731213/94‑1 (MQ=60)
                                                                TCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCA                               <  SRR4302545.2074663/94‑1 (MQ=60)
                                                                TCTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCA                               <  SRR4302545.3994491/94‑1 (MQ=60)
                                                                 CTTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAG                              <  SRR4302545.355618/94‑1 (MQ=60)
                                                                  TTTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGA                             >  SRR4302545.4631946/1‑94 (MQ=60)
                                                                  TTTCATCAGTTTGGCGACGCGATCAAATGCCTCTTCCCAACTGATTTGCTGTCATTTATCAGAACCTGGCGCACGGGAGTCCGGAAACTTCAGA                             >  SRR4302545.9688185/1‑94 (MQ=60)
                                                                   TTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGAC                            <  SRR4302545.9165639/94‑1 (MQ=60)
                                                                   TTCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGAC                            >  SRR4302545.1689610/1‑94 (MQ=60)
                                                                    TCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACG                           >  SRR4302545.6356448/1‑94 (MQ=60)
                                                                    TCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACG                           <  SRR4302545.7978940/94‑1 (MQ=60)
                                                                    TCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACG                           <  SRR4302545.3608042/94‑1 (MQ=60)
                                                                    TCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACG                           <  SRR4302545.4509122/94‑1 (MQ=60)
                                                                    TCATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACG                           >  SRR4302545.7998326/1‑94 (MQ=60)
                                                                     CATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGG                          >  SRR4302545.6873344/1‑94 (MQ=60)
                                                                     CATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGG                          >  SRR4302545.4427977/1‑94 (MQ=60)
                                                                     CATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGG                          <  SRR4302545.1992557/94‑1 (MQ=60)
                                                                     CATCAGTTTGGCGATGCGATCAAATACCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCATGGTATTCCGGAAACTTCAGACGG                          <  SRR4302545.8625350/94‑1 (MQ=60)
                                                                      ATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGC                         <  SRR4302545.1065896/94‑1 (MQ=60)
                                                                      ATCAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGC                         >  SRR4302545.2551109/1‑94 (MQ=60)
                                                                        CAGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTT                       >  SRR4302545.5875146/1‑94 (MQ=60)
                                                                         AGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTT                      <  SRR4302545.103229/94‑1 (MQ=60)
                                                                         AGTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTT                      <  SRR4302545.4150303/94‑1 (MQ=60)
                                                                          GTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTC                     >  SRR4302545.8548416/1‑94 (MQ=60)
                                                                          GTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTC                     <  SRR4302545.957863/94‑1 (MQ=60)
                                                                          GTTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTC                     <  SRR4302545.10542066/94‑1 (MQ=60)
                                                                           TTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCG                    <  SRR4302545.1414120/94‑1 (MQ=60)
                                                                           TTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCG                    <  SRR4302545.8236739/94‑1 (MQ=60)
                                                                           TTTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCG                    >  SRR4302545.1035216/1‑94 (MQ=60)
                                                                            TTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGG                   >  SRR4302545.1609812/1‑94 (MQ=60)
                                                                            TTGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGG                   <  SRR4302545.4694206/94‑1 (MQ=60)
                                                                             TGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGA                  <  SRR4302545.1383304/94‑1 (MQ=60)
                                                                             TGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGA                  <  SRR4302545.1133407/94‑1 (MQ=60)
                                                                             TGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGA                  >  SRR4302545.9966502/1‑94 (MQ=60)
                                                                             TGGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGA                  <  SRR4302545.7834317/94‑1 (MQ=60)
                                                                              GGCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAG                 <  SRR4302545.8619067/94‑1 (MQ=60)
                                                                               GCGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGT                <  SRR4302545.3569181/94‑1 (MQ=60)
                                                                                CGATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTG               <  SRR4302545.5720408/94‑1 (MQ=60)
                                                                                 GATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAATTTCAGACGGCTTTCGGAGTGG              <  SRR4302545.4099647/94‑1 (MQ=60)
                                                                                 GATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTTG              >  SRR4302545.10161326/1‑94 (MQ=60)
                                                                                 GATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGG              <  SRR4302545.6044220/94‑1 (MQ=60)
                                                                                  ATGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGA             >  SRR4302545.5127268/1‑94 (MQ=60)
                                                                                   TGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGAT            <  SRR4302545.326749/94‑1 (MQ=60)
                                                                                   TGCGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGAT            >  SRR4302545.2132768/1‑94 (MQ=60)
                                                                                     CGATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGA          >  SRR4302545.259903/1‑94 (MQ=60)
                                                                                      GATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAA         <  SRR4302545.2626395/94‑1 (MQ=60)
                                                                                      GATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAA         <  SRR4302545.9174108/94‑1 (MQ=60)
                                                                                      GATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAA         >  SRR4302545.9425608/1‑94 (MQ=60)
                                                                                       ATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAA        >  SRR4302545.2074848/1‑94 (MQ=60)
                                                                                       ATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAA        >  SRR4302545.1765410/1‑94 (MQ=60)
                                                                                       ATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAA        >  SRR4302545.7552581/1‑94 (MQ=60)
                                                                                       ATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAA        >  SRR4302545.7602037/1‑94 (MQ=60)
                                                                                       ATCAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCT‑GCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAAT       >  SRR4302545.3884577/1‑94 (MQ=60)
                                                                                        TCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAAT       >  SRR4302545.6264903/1‑94 (MQ=60)
                                                                                         CAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATC      >  SRR4302545.6517383/1‑94 (MQ=60)
                                                                                         CAAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATC      >  SRR4302545.4691046/1‑94 (MQ=60)
                                                                                          AAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCC     >  SRR4302545.2162778/1‑94 (MQ=60)
                                                                                          AAATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCC     >  SRR4302545.3862330/1‑94 (MQ=60)
                                                                                           AATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCA    <  SRR4302545.445964/94‑1 (MQ=60)
                                                                                           AATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCA    <  SRR4302545.244457/94‑1 (MQ=60)
                                                                                           AACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACCGCTTTCGGAGTGGATGAAATCCA    >  SRR4302545.5960415/1‑94 (MQ=60)
                                                                                            ATGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCAC   <  SRR4302545.4048673/94‑1 (MQ=60)
                                                                                             TGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACC  <  SRR4302545.1478126/94‑1 (MQ=60)
                                                                                             TGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACC  <  SRR4302545.1929164/94‑1 (MQ=60)
                                                                                             TGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATGCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACC  <  SRR4302545.1477825/94‑1 (MQ=60)
                                                                                             |                                                                                             
GGAGAGCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACC  >  NC_000913/4082923‑4083109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑