Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,175,949 +G intergenic (+82/‑76) nusG → / → rplK NusG/50S ribosomal subunit protein L11

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,175,9471.G98.6% 1766.1 / 9.5 510intergenic (+80/‑78)nusG/rplKNusG/50S ribosomal subunit protein L11
Reads supporting (aligned to +/- strand):  ref base . (4/3);  new base G (228/275);  total (232/278)
Fisher's exact test for biased strand distribution p-value = 7.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 575 total aligned reads displayed.

TTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAA  >  NC_000913/4175854‑4176035
                                                                                              |                                                                                        
ttGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAt                                                                                           >  2:6796554/1‑94 (MQ=255)
 tGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑g                                                                                         <  2:9770331/94‑1 (MQ=255)
 tGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑g                                                                                         <  2:3990843/94‑1 (MQ=255)
   aaaaaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑ggg                                                                                       <  1:10218492/94‑2 (MQ=255)
   aaaaaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑ggg                                                                                       <  1:7850480/94‑2 (MQ=255)
    aaaaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑gggc                                                                                      <  1:6158328/94‑3 (MQ=255)
    aaaaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑gggc                                                                                      >  2:10369762/1‑92 (MQ=255)
    aaaaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑gggc                                                                                      >  2:5764924/1‑92 (MQ=255)
     aaaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑gggcc                                                                                     >  2:8620720/1‑91 (MQ=255)
      aaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑gggcct                                                                                    <  2:8788651/94‑5 (MQ=255)
      aaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑gggcct                                                                                    >  2:2136957/1‑90 (MQ=255)
        gCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTg                                                                                   <  2:3109430/94‑1 (MQ=255)
         ccTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGGGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGc                                                                                  >  2:8502274/1‑94 (MQ=255)
         ccTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGc                                                                                  >  1:2388464/1‑94 (MQ=255)
          cTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGcc                                                                                 >  2:6062245/1‑94 (MQ=255)
          cTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGcc                                                                                 >  1:40602/1‑94 (MQ=255)
           tAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGccc                                                                                <  2:7481502/94‑1 (MQ=255)
            aaCCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCg                                                                               >  2:766776/1‑94 (MQ=255)
            aaCCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCg                                                                               >  2:7836237/1‑94 (MQ=255)
            aaCCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCg                                                                               <  1:8084312/94‑1 (MQ=255)
             aCCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGt                                                                              >  2:6144604/1‑94 (MQ=255)
              cccAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTa                                                                             >  2:10565083/1‑94 (MQ=255)
                cAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTaaa                                                                           >  1:1765405/1‑94 (MQ=255)
                 aGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTaaac                                                                          >  2:3220182/1‑93 (MQ=255)
                 aGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTaaaa                                                                          >  1:6792482/1‑94 (MQ=255)
                   cGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACg                                                                        <  1:5978782/94‑1 (MQ=255)
                   cGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACg                                                                        <  1:2572419/94‑1 (MQ=255)
                   cGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTCTATGGGCCTTGCCCGTAAAACg                                                                        >  1:4888842/1‑94 (MQ=255)
                    gATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGa                                                                       >  1:727979/1‑94 (MQ=255)
                     aTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAt                                                                      >  1:8909189/1‑94 (MQ=255)
                          aaaaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAtttttt                                                                 <  1:4344946/94‑1 (MQ=255)
                          aaaaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAtttttt                                                                 >  1:7106529/1‑94 (MQ=255)
                             aGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTtata                                                              <  1:3214239/94‑1 (MQ=255)
                             aGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTtata                                                              <  2:5044132/94‑1 (MQ=255)
                             aGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACCATTTTTtata                                                              <  2:6864851/94‑1 (MQ=255)
                               cggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATc                                                            <  1:3246433/94‑1 (MQ=255)
                                ggcgATTTAAGCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGTCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCa                                                           <  1:6254832/94‑1 (MQ=255)
                                 gcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCAc                                                          <  2:1154110/94‑1 (MQ=255)
                                   gATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACgg                                                        >  2:9015867/1‑94 (MQ=255)
                                   gATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACgg                                                        >  1:3528329/1‑94 (MQ=255)
                                    aTTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACggg                                                       <  1:5180494/94‑1 (MQ=255)
                                      ttAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGa                                                     <  1:9822997/94‑1 (MQ=255)
                                        aaTCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGc                                                   >  1:1651812/1‑94 (MQ=255)
                                         aTCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGcc                                                  <  2:3723780/94‑1 (MQ=255)
                                          tCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACAATTTTTTATATCTCGGGGAGCct                                                 <  2:5488231/94‑1 (MQ=255)
                                           cGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCctc                                                >  2:8341922/1‑94 (MQ=255)
                                            ggtGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCctct                                               <  1:5399137/92‑1 (MQ=255)
                                            gTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCctct                                               <  1:1914704/94‑1 (MQ=255)
                                            gTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCctct                                               >  2:6454997/1‑94 (MQ=255)
                                            gTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCctct                                               <  1:10018841/94‑1 (MQ=255)
                                             ttGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCtctc                                              >  2:496329/1‑94 (MQ=255)
                                              tGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCa                                             >  1:2040116/1‑94 (MQ=255)
                                               gCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCag                                            >  2:274844/1‑94 (MQ=255)
                                                cacaAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCaga                                           >  2:5899742/1‑94 (MQ=255)
                                                   aaGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGc                                        <  2:10060523/94‑1 (MQ=255)
                                                   aaGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGc                                        <  2:2583776/94‑1 (MQ=255)
                                                    aGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAACCGATTTTTTATATCACGGGGAGCCTCTCAGAGGCg                                       <  1:8278261/94‑1 (MQ=255)
                                                    aGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCg                                       <  2:4833001/94‑1 (MQ=255)
                                                    aGGCGTGAGATTGGAATACAATGTCGCGTCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCg                                       <  2:7312284/94‑1 (MQ=255)
                                                     ggCGTGAGATTGGAATACACTTTCGCGCCTTTTGTTTTTATGGGGCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGt                                      >  1:6896143/1‑94 (MQ=255)
                                                      gCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGtt                                     <  1:6534563/94‑1 (MQ=255)
                                                      gCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGtt                                     <  2:2324181/94‑1 (MQ=255)
                                                        gTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGttat                                   <  1:4497917/94‑1 (MQ=255)
                                                         tGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGttatt                                  <  1:3463530/94‑1 (MQ=255)
                                                         tGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGttatt                                  <  1:1514508/94‑1 (MQ=255)
                                                          gagaTTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGttatta                                 <  2:9239682/94‑1 (MQ=255)
                                                           agaTTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTAc                                >  1:2940529/1‑94 (MQ=255)
                                                           agaTTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTAc                                <  2:4641791/94‑1 (MQ=255)
                                                           agaTTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGCGAGCCTCTCAGAGGCGTTATTAc                                <  2:1923806/94‑1 (MQ=255)
                                                            gaTTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTAcc                               >  2:7135306/1‑94 (MQ=255)
                                                             aTTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTAccc                              >  1:9275730/1‑94 (MQ=255)
                                                              ttGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCa                             <  2:5308144/94‑1 (MQ=255)
                                                               tGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCaa                            >  2:3590215/1‑94 (MQ=255)
                                                                 gAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACt                          <  1:7758793/94‑1 (MQ=255)
                                                                 gAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACt                          >  1:7476024/1‑94 (MQ=255)
                                                                  aaTACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACtt                         >  1:9752282/1‑94 (MQ=255)
                                                                    tACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGa                       >  1:1357482/1‑94 (MQ=255)
                                                                     aCAATTTCGCGCCTTTTGTTGTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAg                      <  2:1549750/94‑1 (MQ=255)
                                                                      cAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTTCCCAACTTGAgg                     <  2:1480809/94‑1 (MQ=255)
                                                                      cAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAgg                     >  2:4406176/1‑94 (MQ=255)
                                                                       aaTTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGa                    <  2:4153422/94‑1 (MQ=255)
                                                                        aTTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGaa                   <  1:279373/94‑1 (MQ=255)
                                                                        aTTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGaa                   <  2:3833104/94‑1 (MQ=255)
                                                                         tttCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAAt                  >  1:10502602/1‑94 (MQ=255)
                                                                          ttCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAAtt                 >  2:8057580/1‑94 (MQ=255)
                                                                          ttCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAAtt                 >  2:5223038/1‑94 (MQ=255)
                                                                           tCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAAttt                <  1:8713599/94‑1 (MQ=255)
                                                                            cgcgCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTta               >  1:9613327/1‑94 (MQ=255)
                                                                            cgcgCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGATATTACCCAACTTGAGGAATTta               >  1:5829737/1‑94 (MQ=255)
                                                                              cgcCTTTTGTTTTTATGGGACTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTtata             >  2:2874036/1‑94 (MQ=255)
                                                                               gcCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATaa            >  2:6574383/1‑94 (MQ=255)
                                                                               gcCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATaa            <  2:1230830/94‑1 (MQ=255)
                                                                                ccTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAAt           <  1:2256308/94‑1 (MQ=255)
                                                                                 cTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATg          >  2:9489616/1‑94 (MQ=255)
                                                                                  ttttGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATgg         <  1:3829198/94‑1 (MQ=255)
                                                                                   tttgtttgTATGGGCCTTGCACGTAAAACGATTGTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGc        <  2:10262891/94‑1 (MQ=255)
                                                                                     tGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCCGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTa      >  1:7662645/1‑94 (MQ=255)
                                                                                       tttttATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAg    <  1:8477520/94‑1 (MQ=255)
                                                                                        ttttATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGa   >  1:9486067/1‑94 (MQ=255)
                                                                                         tttATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGaa  >  1:6070452/1‑94 (MQ=255)
                                                                                              |                                                                                        
TTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAA  >  NC_000913/4175854‑4176035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 329 total aligned reads displayed.
GGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGACGG  >  NC_000913/4177255‑4177441
                                                                                             |                                                                                             
GGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGG                                                                                               >  SRR4302545.826577/1‑94 (MQ=60)
GGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGG                                                                                               <  SRR4302545.4437458/94‑1 (MQ=60)
GGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGG                                                                                               >  SRR4302545.7045778/1‑94 (MQ=60)
 GCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGT                                                                                              <  SRR4302545.5081683/94‑1 (MQ=60)
  CGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTA                                                                                             >  SRR4302545.1874263/1‑94 (MQ=60)
  CGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTA                                                                                             <  SRR4302545.4050127/94‑1 (MQ=60)
  CGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTA                                                                                             >  SRR4302545.524691/1‑94 (MQ=60)
   GGCAACTGAGTGAGTCGCCAGGAGCATTGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAG                                                                                            >  SRR4302545.9686886/1‑94 (MQ=60)
   GGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAG                                                                                            >  SRR4302545.6398004/1‑94 (MQ=60)
   GGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAG                                                                                            <  SRR4302545.8231301/94‑1 (MQ=60)
   GGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAG                                                                                            <  SRR4302545.4775031/94‑1 (MQ=60)
   GGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAG                                                                                            <  SRR4302545.652914/94‑1 (MQ=60)
    GCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGA                                                                                           <  SRR4302545.8271330/94‑1 (MQ=60)
    GCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGA                                                                                           >  SRR4302545.10097706/1‑94 (MQ=60)
     CAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCATCGCCGTCACAAGTTGAATAGCGACGGGTAGAA                                                                                          <  SRR4302545.7557209/94‑1 (MQ=60)
      AACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAA                                                                                         >  SRR4302545.3817959/1‑94 (MQ=60)
        CTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAG                                                                                       <  SRR4302545.10633276/94‑1 (MQ=60)
        CTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAG                                                                                       <  SRR4302545.10661536/94‑1 (MQ=60)
         TGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGA                                                                                      >  SRR4302545.3237145/1‑94 (MQ=60)
           AGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATT                                                                                    >  SRR4302545.8037237/1‑94 (MQ=60)
           AGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATT                                                                                    >  SRR4302545.5593034/1‑94 (MQ=60)
            GTTAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTT                                                                                   <  SRR4302545.7924357/94‑1 (MQ=60)
            GTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTT                                                                                   <  SRR4302545.10704965/94‑1 (MQ=60)
             TGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTG                                                                                  >  SRR4302545.6440718/1‑94 (MQ=60)
             TGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTG                                                                                  <  SRR4302545.10141691/94‑1 (MQ=60)
             TGAGTCGCAAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTG                                                                                  <  SRR4302545.6210362/94‑1 (MQ=60)
              GAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGT                                                                                 <  SRR4302545.3062535/94‑1 (MQ=60)
              GAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGT                                                                                 <  SRR4302545.3045818/94‑1 (MQ=60)
               AGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTT                                                                                <  SRR4302545.6179707/94‑1 (MQ=60)
               AGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTT                                                                                <  SRR4302545.4803711/94‑1 (MQ=60)
               AGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTT                                                                                <  SRR4302545.8616368/94‑1 (MQ=60)
               AGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTT                                                                                >  SRR4302545.9015847/1‑94 (MQ=60)
               AGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTT                                                                                <  SRR4302545.3237954/94‑1 (MQ=60)
               AGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTT                                                                                >  SRR4302545.10211339/1‑94 (MQ=60)
               AGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTT                                                                                <  SRR4302545.10482440/94‑1 (MQ=60)
               AGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTT                                                                                >  SRR4302545.10606121/1‑94 (MQ=60)
               AGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTT                                                                                >  SRR4302545.9015849/1‑94 (MQ=60)
                 TCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCG                                                                              <  SRR4302545.4527703/94‑1 (MQ=60)
                 TCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCG                                                                              <  SRR4302545.10758164/94‑1 (MQ=60)
                 TCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCG                                                                              <  SRR4302545.7803943/94‑1 (MQ=60)
                  CGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAACAGATTTGTTCGT                                                                             >  SRR4302545.3657943/1‑94 (MQ=60)
                  CGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGT                                                                             >  SRR4302545.3983368/1‑94 (MQ=60)
                   GCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTT                                                                            <  SRR4302545.10077507/94‑1 (MQ=60)
                   GCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTT                                                                            <  SRR4302545.5802674/94‑1 (MQ=60)
                    CCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTG                                                                           >  SRR4302545.1384145/1‑94 (MQ=60)
                    CCAGGAGCATAGCTAACTAGGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTG                                                                           >  SRR4302545.10671324/1‑94 (MQ=60)
                     CAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGG                                                                          <  SRR4302545.4315928/94‑1 (MQ=60)
                     CAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGG                                                                          >  SRR4302545.6109380/1‑94 (MQ=60)
                     CAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGG                                                                          <  SRR4302545.3366035/94‑1 (MQ=60)
                     CAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGG                                                                          >  SRR4302545.2081582/1‑94 (MQ=60)
                      AGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGA                                                                         <  SRR4302545.2704256/94‑1 (MQ=60)
                      AGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGA                                                                         >  SRR4302545.2227978/1‑94 (MQ=60)
                      AGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGA                                                                         >  SRR4302545.9771365/1‑94 (MQ=60)
                      AGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGA                                                                         <  SRR4302545.596809/94‑1 (MQ=60)
                       GGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAG                                                                        <  SRR4302545.8811256/94‑1 (MQ=60)
                       GGAGCATAGCTAACTATGTGACTGATGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAG                                                                        <  SRR4302545.6538294/94‑1 (MQ=60)
                        GAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGC                                                                       <  SRR4302545.2196257/94‑1 (MQ=60)
                        GAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGC                                                                       >  SRR4302545.2162370/1‑94 (MQ=60)
                        GAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGC                                                                       >  SRR4302545.5053686/1‑94 (MQ=60)
                         AGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCC                                                                      <  SRR4302545.958478/94‑1 (MQ=60)
                         AGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCC                                                                      <  SRR4302545.10646844/94‑1 (MQ=60)
                         AGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCC                                                                      <  SRR4302545.6300795/94‑1 (MQ=60)
                         AGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCC                                                                      >  SRR4302545.6606444/1‑94 (MQ=60)
                         AGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCC                                                                      <  SRR4302545.6681074/94‑1 (MQ=60)
                         AGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCC                                                                      <  SRR4302545.3541913/94‑1 (MQ=60)
                           CATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTG                                                                    <  SRR4302545.2884697/94‑1 (MQ=60)
                           CATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTG                                                                    >  SRR4302545.9624356/1‑94 (MQ=60)
                           CATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTG                                                                    <  SRR4302545.7123279/94‑1 (MQ=60)
                           CATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTG                                                                    <  SRR4302545.1686037/94‑1 (MQ=60)
                           CATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGATGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTG                                                                    >  SRR4302545.6062680/1‑94 (MQ=60)
                            ATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGG                                                                   <  SRR4302545.7190223/94‑1 (MQ=60)
                            ATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGG                                                                   >  SRR4302545.2917715/1‑94 (MQ=60)
                            ATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGG                                                                   >  SRR4302545.8709232/1‑94 (MQ=60)
                            ATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTAG                                                                   >  SRR4302545.234993/1‑94 (MQ=60)
                            ATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGG                                                                   <  SRR4302545.7543408/94‑1 (MQ=60)
                             TAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGC                                                                  >  SRR4302545.5796964/1‑94 (MQ=60)
                              AGCTAACTATGTGACTGGTGCGAATGAAGGAAGCGAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCC                                                                 <  SRR4302545.10810564/94‑1 (MQ=60)
                              AGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCC                                                                 <  SRR4302545.10206268/94‑1 (MQ=60)
                               GCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCT                                                                >  SRR4302545.5915448/1‑94 (MQ=60)
                                CTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGTTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTA                                                               <  SRR4302545.7648628/94‑1 (MQ=60)
                                CTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTA                                                               >  SRR4302545.7296977/1‑94 (MQ=60)
                                CTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTA                                                               <  SRR4302545.7648629/94‑1 (MQ=60)
                                 TAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTAT                                                              >  SRR4302545.349106/1‑94 (MQ=60)
                                  AACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATC                                                             >  SRR4302545.785907/1‑94 (MQ=60)
                                   ACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCC                                                            <  SRR4302545.9625344/94‑1 (MQ=60)
                                   ACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCC                                                            <  SRR4302545.1804966/94‑1 (MQ=60)
                                    CTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCA                                                           >  SRR4302545.3843907/1‑94 (MQ=60)
                                    CTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCA                                                           <  SRR4302545.9554158/94‑1 (MQ=60)
                                    CTATGTGACTGGTGCGAATGAAGAAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCA                                                           <  SRR4302545.10812562/94‑1 (MQ=60)
                                    CTATGTGACTGGGGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCA                                                           >  SRR4302545.8221676/1‑94 (MQ=60)
                                    ATATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCA                                                           <  SRR4302545.7297710/94‑1 (MQ=60)
                                     TATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAG                                                          >  SRR4302545.4589015/1‑94 (MQ=60)
                                     TATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAG                                                          >  SRR4302545.4422324/1‑94 (MQ=60)
                                      ATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGG                                                         <  SRR4302545.5060054/94‑1 (MQ=60)
                                         TGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCT                                                      <  SRR4302545.9645507/94‑1 (MQ=60)
                                         TGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCT                                                      >  SRR4302545.880644/1‑94 (MQ=60)
                                         TGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCT                                                      <  SRR4302545.10823846/94‑1 (MQ=60)
                                         TGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCC                                                      >  SRR4302545.7665498/1‑94 (MQ=60)
                                           ACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCC                                                    <  SRR4302545.7409614/94‑1 (MQ=60)
                                           ACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCC                                                    <  SRR4302545.9245918/94‑1 (MQ=60)
                                           ACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCC                                                    >  SRR4302545.4455329/1‑94 (MQ=60)
                                           ACTGGTGCGAATGAAGGAAGCCAACACCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCCGGCCTCC                                                    <  SRR4302545.1967919/94‑1 (MQ=60)
                                            CTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCG                                                   <  SRR4302545.10348701/94‑1 (MQ=60)
                                            CTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCG                                                   >  SRR4302545.2678789/1‑94 (MQ=60)
                                            CTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCG                                                   >  SRR4302545.5325763/1‑94 (MQ=60)
                                             TGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGT                                                  <  SRR4302545.7777218/94‑1 (MQ=60)
                                               GTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCG                                                >  SRR4302545.1724766/1‑94 (MQ=60)
                                                TGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGA                                               >  SRR4302545.9326189/1‑94 (MQ=60)
                                                  CGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAG                                             >  SRR4302545.3272848/1‑94 (MQ=60)
                                                   GAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGA                                            <  SRR4302545.6434763/94‑1 (MQ=60)
                                                   GAATGAAGGAAGCCAACGACGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGC                                            >  SRR4302545.10765827/1‑94 (MQ=60)
                                                   GAATGAAGGAAGCCAACGACGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGA                                            >  SRR4302545.8270100/1‑94 (MQ=60)
                                                    AATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTGTCCAGGCCTCCGTCGAAGAC                                           >  SRR4302545.6090553/1‑94 (MQ=60)
                                                    AATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGAC                                           <  SRR4302545.802935/94‑1 (MQ=60)
                                                    AATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGAC                                           <  SRR4302545.5741658/94‑1 (MQ=60)
                                                     ATAAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACC                                          <  SRR4302545.773712/94‑1 (MQ=60)
                                                      TGAAGGAAGCCAGCGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCGGGCCTATCCAGGCCTCCGTCGAAGACCG                                         <  SRR4302545.3157768/94‑1 (MQ=60)
                                                      TGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCG                                         >  SRR4302545.840106/1‑94 (MQ=60)
                                                      TGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCG                                         >  SRR4302545.8669529/1‑94 (MQ=60)
                                                       GAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGC                                        <  SRR4302545.5719710/94‑1 (MQ=60)
                                                        AAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCA                                       <  SRR4302545.5310193/94‑1 (MQ=60)
                                                        AAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCA                                       <  SRR4302545.7451563/94‑1 (MQ=60)
                                                         AGGAAGCCAACGCCGTCACAAGCTGAATAGCTACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAG                                      >  SRR4302545.4523847/1‑94 (MQ=60)
                                                         AGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAG                                      >  SRR4302545.1034802/1‑94 (MQ=60)
                                                          GGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGG                                     <  SRR4302545.4723427/94‑1 (MQ=60)
                                                          AGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGG                                     <  SRR4302545.8256286/94‑1 (MQ=60)
                                                           GAAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCATGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGA                                    <  SRR4302545.2109205/94‑1 (MQ=60)
                                                            AAGCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAG                                   >  SRR4302545.4250226/1‑94 (MQ=60)
                                                             AGCCAACACCGTCACAAGCTTAATAGCGACGGGTTGAAAATATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGT                                  <  SRR4302545.3099511/94‑1 (MQ=60)
                                                              GCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTT                                 <  SRR4302545.5078690/94‑1 (MQ=60)
                                                              GCCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTT                                 >  SRR4302545.840427/1‑94 (MQ=60)
                                                               CCAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTT                                >  SRR4302545.2571165/1‑94 (MQ=60)
                                                                CAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTC                               <  SRR4302545.2935705/94‑1 (MQ=60)
                                                                CAACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTC                               <  SRR4302545.5704112/94‑1 (MQ=60)
                                                                 AACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCG                              >  SRR4302545.10262364/1‑94 (MQ=60)
                                                                 AACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCG                              >  SRR4302545.7957795/1‑94 (MQ=60)
                                                                 AACGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTAGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCG                              >  SRR4302545.6874015/1‑94 (MQ=60)
                                                                   CGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCA                            >  SRR4302545.7539783/1‑94 (MQ=60)
                                                                   CGCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCA                            >  SRR4302545.2459683/1‑94 (MQ=60)
                                                                    GCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAA                           >  SRR4302545.4319395/1‑94 (MQ=60)
                                                                    GCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAA                           >  SRR4302545.8108752/1‑94 (MQ=60)
                                                                    GCCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAA                           >  SRR4302545.5789982/1‑94 (MQ=60)
                                                                     CCGTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAG                          <  SRR4302545.9298476/94‑1 (MQ=60)
                                                                       GTCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAA                        >  SRR4302545.572881/1‑94 (MQ=60)
                                                                        TCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAA                       <  SRR4302545.9375165/94‑1 (MQ=60)
                                                                        TCACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAA                       >  SRR4302545.5931567/1‑94 (MQ=60)
                                                                         CACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAACC                      <  SRR4302545.8494220/94‑1 (MQ=60)
                                                                         CACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAAC                      >  SRR4302545.10389965/1‑94 (MQ=60)
                                                                         CACAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAAC                      >  SRR4302545.9781391/1‑94 (MQ=60)
                                                                          ACAAGCTGAAGAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACT                     <  SRR4302545.2811910/94‑1 (MQ=60)
                                                                           CAAGCTGACTAGCGACGTGTAGAAAAGATTTGTTCGTTGGAGACTGGCCTATCCAGGCCTCCGCCGAAGCCCGCCGGGGTTTCGCAAGAAACTT                    <  SRR4302545.3497645/94‑1 (MQ=60)
                                                                           CAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTT                    <  SRR4302545.8789061/94‑1 (MQ=60)
                                                                           CAAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTT                    >  SRR4302545.6707882/1‑94 (MQ=60)
                                                                            AAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTA                   >  SRR4302545.8783822/1‑94 (MQ=60)
                                                                            AAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTA                   <  SRR4302545.10073522/94‑1 (MQ=60)
                                                                            AAGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTA                   <  SRR4302545.9020756/94‑1 (MQ=60)
                                                                             AGCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAA                  >  SRR4302545.6277072/1‑94 (MQ=60)
                                                                              ggtggATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAAT                 <  SRR4302545.4087824/89‑1 (MQ=60)
                                                                              GCTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCACGAAACTTAAT                 <  SRR4302545.7240474/94‑1 (MQ=60)
                                                                               CTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTTGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATC                >  SRR4302545.1518537/1‑94 (MQ=60)
                                                                               CTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATC                >  SRR4302545.3691190/1‑94 (MQ=60)
                                                                               CTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATC                >  SRR4302545.8305483/1‑94 (MQ=60)
                                                                               CTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATC                >  SRR4302545.2478405/1‑94 (MQ=60)
                                                                               CTGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATC                >  SRR4302545.5468384/1‑94 (MQ=60)
                                                                                TGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCC               <  SRR4302545.5763867/94‑1 (MQ=60)
                                                                                TGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCC               >  SRR4302545.9138515/1‑94 (MQ=60)
                                                                                TGAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCC               >  SRR4302545.7371233/1‑94 (MQ=60)
                                                                                 GAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCC              <  SRR4302545.6302010/94‑1 (MQ=60)
                                                                                 GAATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCC              >  SRR4302545.8673884/1‑94 (MQ=60)
                                                                                  AATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCC             <  SRR4302545.2080875/94‑1 (MQ=60)
                                                                                   ATAGCGACGGGTAGAAAATATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCT            <  SRR4302545.2038550/94‑1 (MQ=60)
                                                                                   ATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCT            <  SRR4302545.3468815/94‑1 (MQ=60)
                                                                                   ATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCT            >  SRR4302545.5911938/1‑94 (MQ=60)
                                                                                   ATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCT            <  SRR4302545.327275/94‑1 (MQ=60)
                                                                                   ATAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACCTAATACCCT            >  SRR4302545.10526899/1‑94 (MQ=60)
                                                                                    TAGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTG           <  SRR4302545.6220111/94‑1 (MQ=60)
                                                                                     AGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGC          >  SRR4302545.8748730/1‑94 (MQ=60)
                                                                                     AGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGC          <  SRR4302545.1278949/94‑1 (MQ=60)
                                                                                     AGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGC          >  SRR4302545.9485737/1‑94 (MQ=60)
                                                                                     AGCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGC          <  SRR4302545.1562958/94‑1 (MQ=60)
                                                                                      GCGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGGG         <  SRR4302545.6889920/94‑1 (MQ=60)
                                                                                       CGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGT        >  SRR4302545.9884938/1‑94 (MQ=60)
                                                                                       CGACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGT        <  SRR4302545.18456/94‑1 (MQ=60)
                                                                                        GACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTA       <  SRR4302545.8471607/94‑1 (MQ=60)
                                                                                        GACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTA       >  SRR4302545.7721236/1‑94 (MQ=60)
                                                                                        GACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTA       >  SRR4302545.4594720/1‑94 (MQ=60)
                                                                                         ACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAG      <  SRR4302545.6840958/94‑1 (MQ=60)
                                                                                         ACGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAG      <  SRR4302545.7187225/94‑1 (MQ=60)
                                                                                          CGGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGTGTAGA     <  SRR4302545.6989477/94‑1 (MQ=60)
                                                                                           GGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGTGTTTCGCAAGAAACTTAATCCCCTGCGTAGAC    <  SRR4302545.9185596/94‑1 (MQ=60)
                                                                                           GGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGAC    <  SRR4302545.496437/94‑1 (MQ=60)
                                                                                           GGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGAC    <  SRR4302545.483359/94‑1 (MQ=60)
                                                                                           GGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGAC    >  SRR4302545.2356455/1‑94 (MQ=60)
                                                                                           GGGTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGAC    >  SRR4302545.1021115/1‑94 (MQ=60)
                                                                                           GGGTAGAAAAGATGTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGTGTTTCGCAAGAAACTTAATCCCCGGCGTAGAC    <  SRR4302545.9185595/94‑1 (MQ=60)
                                                                                            GATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGGTTCGCAAGAAACTTAATCCCCTGCGTAGACG   >  SRR4302545.9244759/1‑94 (MQ=60)
                                                                                             GTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGACGG  >  SRR4302545.2581789/1‑94 (MQ=60)
                                                                                             GTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGACGG  <  SRR4302545.1368162/94‑1 (MQ=60)
                                                                                             GTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGACGG  <  SRR4302545.7162401/94‑1 (MQ=60)
                                                                                             GTAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGACGG  >  SRR4302545.2157693/1‑94 (MQ=60)
                                                                                             |                                                                                             
GGCGGCAACTGAGTGAGTCGCCAGGAGCATAGCTAACTATGTGACTGGTGCGAATGAAGGAAGCCAACGCCGTCACAAGCTGAATAGCGACGGATAGAAAAGATTTGTTCGTTGGAGCCTGGCCTATCCAGGCCTCCGTCGAAGACCGCAGGAGTTTCGCAAGAAACTTAATCCCCTGCGTAGACGG  >  NC_000913/4177255‑4177441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑