Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,272,173 A→G Q157R (CAG→CGG)  ssb → ssDNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,272,1730AG99.1% 1496.5 / 0.7 436Q157R (CAG→CGG) ssbssDNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base G (190/242);  total (191/245)
Fisher's exact test for biased strand distribution p-value = 6.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01

Only 100 of 487 total aligned reads displayed.

ATCGGTGGTGGTCAGCCGCAGGGCGGTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTA  >  NC_000913/4272082‑4272263
                                                                                           |                                                                                          
atCGGTGGTGGTCAGCCGCAGGGCGGTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCgcggc                                                                                          >  2:6466516/1‑94 (MQ=255)
  cGGTGGGAGTCAGCCGCAGGGCGGTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGcggcag                                                                                        <  2:7844808/94‑1 (MQ=255)
    gtggtggtCAGCCGCAGGGCGGTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTc                                                                                      >  1:7278829/1‑94 (MQ=255)
      ggtggtCAGCCGCAGGGCGGTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCg                                                                                    <  2:6395186/94‑1 (MQ=255)
       gtggtCAGCCGCAGGGCGGTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGc                                                                                   <  2:1590145/94‑1 (MQ=255)
         ggtCAGCCGCAGGGCGGTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTc                                                                                 >  2:2576593/1‑94 (MQ=255)
         ggtCAGCCGCAGGGCGGTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTc                                                                                 <  1:7842749/94‑1 (MQ=255)
           gcAGCCGCGGGGCGGTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCATCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCg                                                                               <  1:8775658/93‑1 (MQ=255)
            cAGCCGCAGGGCGGTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCgg                                                                              <  1:3620140/94‑1 (MQ=255)
             aGCCGCAGGGCGGTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGc                                                                             >  1:1004588/1‑94 (MQ=255)
              gCCGCAGGGCGGTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCa                                                                            <  1:2166391/94‑1 (MQ=255)
               ccGCAGGGCGGTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAg                                                                           >  2:9691683/1‑94 (MQ=255)
               ccGCAGGGCGGTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAg                                                                           >  2:6284762/1‑94 (MQ=255)
                cGCAGGGCGGTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAgc                                                                          >  1:6745185/1‑94 (MQ=255)
                cGCAGGGCGGTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAgc                                                                          <  2:218331/94‑1 (MQ=255)
                cGCAGGGCGGTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAgc                                                                          >  2:8825050/1‑94 (MQ=255)
                cGCAGGGCGGTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAgc                                                                          >  2:2071862/1‑94 (MQ=255)
                 gCAGGGCGGTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAgcg                                                                         >  2:7310961/1‑94 (MQ=255)
                  cAGGGCGGTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAgcgc                                                                        >  1:8382860/1‑94 (MQ=255)
                   aGGGCGGTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGcc                                                                       >  1:2737601/1‑94 (MQ=255)
                       gggTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCt                                                                   <  1:23633/93‑1 (MQ=255)
                           tGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACg                                                               <  1:8044167/94‑1 (MQ=255)
                            ggggTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGa                                                              <  2:7684132/94‑1 (MQ=255)
                              ggTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAgc                                                            <  2:8195304/94‑1 (MQ=255)
                              ggTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAgc                                                            >  1:344761/1‑94 (MQ=255)
                               gTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAgcc                                                           <  1:5064762/94‑1 (MQ=255)
                               gTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAgcc                                                           >  2:5369236/1‑94 (MQ=255)
                                tCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGTCAGCGCCGGCTAACGAgccc                                                          >  2:2606851/1‑93 (MQ=255)
                                tCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAgccg                                                          >  2:7545635/1‑94 (MQ=255)
                                  aGCGTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAgccgcc                                                        <  2:3903281/94‑1 (MQ=255)
                                  aGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAgccgcc                                                        >  1:1033313/1‑94 (MQ=255)
                                  aGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAgccgcc                                                        >  2:5303270/1‑94 (MQ=255)
                                  aGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAgccgcc                                                        <  1:8883943/94‑1 (MQ=255)
                                  aGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAgccgcc                                                        >  2:8492145/1‑94 (MQ=255)
                                   gCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGccgccg                                                       <  2:10443693/94‑1 (MQ=255)
                                    ccTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGa                                                      >  1:10546372/1‑94 (MQ=255)
                                     cTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGAt                                                     <  1:6288379/94‑1 (MQ=255)
                                     cTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGAt                                                     >  2:3713479/1‑94 (MQ=255)
                                     cTCAGCAGCCGCAGGGTGGCAATCAGTTCAGAGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCCAAACAGCCGCCGAt                                                     >  2:5023824/1‑94 (MQ=255)
                                      tCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATg                                                    >  1:3909536/1‑94 (MQ=255)
                                       cagcagCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATgg                                                   <  1:7001231/94‑1 (MQ=255)
                                       cagcagCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATgg                                                   >  1:5764927/1‑94 (MQ=255)
                                        agcagcCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGa                                                  >  1:3290259/1‑94 (MQ=255)
                                        agcagcCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGa                                                  >  1:1414314/1‑94 (MQ=255)
                                          cagcCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACt                                                <  1:4737041/94‑1 (MQ=255)
                                            gcCGCAGGGTGGCAATCGGTTCAGCGGCGGCGCGCAGTCTCTCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACttt                                              <  1:4490862/94‑1 (MQ=255)
                                            gcCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACttt                                              <  2:6648361/94‑1 (MQ=255)
                                             ccGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTtg                                             <  2:8602335/94‑1 (MQ=255)
                                              cGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTtga                                            >  2:5822543/1‑94 (MQ=255)
                                                cAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTtgatg                                          >  2:3399556/1‑94 (MQ=255)
                                                 aGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTtgatga                                         >  2:6173177/1‑94 (MQ=255)
                                                  gggTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTtgatgat                                        >  1:7994357/1‑94 (MQ=255)
                                                   ggTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTtgatgatg                                       >  2:4964284/1‑94 (MQ=255)
                                                   ggTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTtgatgatg                                       <  2:4331524/94‑1 (MQ=255)
                                                    gTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTtgatgatga                                      >  1:5294218/1‑94 (MQ=255)
                                                    gTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTtgatgatga                                      <  1:2062679/94‑1 (MQ=255)
                                                     tGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGAc                                     <  2:9880739/94‑1 (MQ=255)
                                                      ggCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACa                                    >  2:2372244/1‑94 (MQ=255)
                                                       gCAATCGGTTCAGCGACGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTACGGCAGCGCCGTCTAACGAGCCGCCGATGGACTatgatgatgtcct                                   <  1:9393222/94‑5 (MQ=255)
                                                       gCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGTCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACAt                                   <  2:2838085/94‑1 (MQ=255)
                                                        cAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACAtt                                  >  2:460603/1‑94 (MQ=255)
                                                         aaTCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTc                                 >  1:8613795/1‑94 (MQ=255)
                                                           tCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCg                               <  1:6171089/94‑1 (MQ=255)
                                                             aGTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGtt                             >  1:5562105/1‑94 (MQ=255)
                                                              gTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTc                            >  2:1313148/1‑94 (MQ=255)
                                                              gTTCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTc                            <  2:5535192/94‑1 (MQ=255)
                                                               ttCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGCTGGACTTTGATGATGACATTCCGTTCt                           >  2:3191435/1‑94 (MQ=255)
                                                               ttCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCt                           <  1:10384075/94‑1 (MQ=255)
                                                               ttCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCt                           <  1:9551167/94‑1 (MQ=255)
                                                                tCAGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTg                          <  1:5634049/94‑1 (MQ=255)
                                                                  aGCGGCGGCGCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGAt                        <  2:926718/94‑1 (MQ=255)
                                                                   gcggcggcgCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGAtt                       >  2:10043982/1‑94 (MQ=255)
                                                                    gggcggcgCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGAttt                      <  1:4182609/93‑1 (MQ=255)
                                                                    cggcggcgCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGAttt                      <  1:649709/94‑1 (MQ=255)
                                                                     ggcggcgCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTg                     >  1:407896/1‑94 (MQ=255)
                                                                      gcggcgCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGt                    <  2:1084781/94‑1 (MQ=255)
                                                                      gcggcgCGCAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGt                    <  1:9240314/94‑1 (MQ=255)
                                                                          cgcgcAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCAtt                >  1:4306874/1‑94 (MQ=255)
                                                                          cgcgcAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCAtt                <  2:6882605/94‑1 (MQ=255)
                                                                          cgcgcAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCAtt                <  1:9634361/94‑1 (MQ=255)
                                                                           gcgcAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTa               >  1:3769408/1‑94 (MQ=255)
                                                                           gcgcAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTa               <  1:1709490/94‑1 (MQ=255)
                                                                           gcgcAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTa               <  1:8965831/94‑1 (MQ=255)
                                                                           gcgcAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTa               >  2:9095947/1‑94 (MQ=255)
                                                                            cgcAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTaa              <  2:1018442/94‑1 (MQ=255)
                                                                             gcAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTaaa             <  2:4087133/94‑1 (MQ=255)
                                                                              cAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTaaaa            <  2:700380/94‑1 (MQ=255)
                                                                              cAGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTaaaa            <  1:7458455/94‑1 (MQ=255)
                                                                               aGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAAc           >  1:4912144/1‑94 (MQ=255)
                                                                               aGTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAAc           <  2:839215/94‑1 (MQ=255)
                                                                                gTCTCGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACa          <  1:7688071/94‑1 (MQ=255)
                                                                                   tcGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATa       <  1:3128849/94‑1 (MQ=255)
                                                                                   tcGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATa       >  2:2708775/1‑94 (MQ=255)
                                                                                   ccGCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATa       <  1:2586815/93‑1 (MQ=255)
                                                                                     gCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTTTGATTTGTCATTAAAACAATAgg     <  2:1935981/94‑1 (MQ=255)
                                                                                     gCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAgg     >  1:604014/1‑94 (MQ=255)
                                                                                     gCCCGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAgg     <  2:352954/94‑1 (MQ=255)
                                                                                      cccGCGGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGt    <  1:1138343/94‑1 (MQ=255)
                                                                                        cgcgGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTta  >  1:9745687/1‑94 (MQ=255)
                                                                                        cgcgGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTta  >  2:9447448/1‑94 (MQ=255)
                                                                                           |                                                                                          
ATCGGTGGTGGTCAGCCGCAGGGCGGTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTA  >  NC_000913/4272082‑4272263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 299 total aligned reads displayed.
GTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGC  >  NC_000913/4276397‑4276583
                                                                                             |                                                                                             
GTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCG                                                                                               >  SRR4302545.9583099/1‑94 (MQ=60)
 TGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCCCGGTGTGGCGCACGGCGC                                                                                              >  SRR4302545.1894603/1‑94 (MQ=60)
 TGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGC                                                                                              <  SRR4302545.7675877/94‑1 (MQ=60)
 TGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGC                                                                                              >  SRR4302545.2345181/1‑94 (MQ=60)
 TGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGC                                                                                              >  SRR4302545.820443/1‑94 (MQ=60)
  GTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCG                                                                                             >  SRR4302545.1772774/1‑94 (MQ=60)
   TTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGG                                                                                            <  SRR4302545.7200667/94‑1 (MQ=60)
   TTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGG                                                                                            <  SRR4302545.5705199/94‑1 (MQ=60)
   TTCCTGATGGGTGTGATATTTACGGTAATTTCTGCCACGGGTAACCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGG                                                                                            <  SRR4302545.9665208/94‑1 (MQ=60)
    TCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGG                                                                                           >  SRR4302545.10882492/1‑94 (MQ=60)
    TCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGG                                                                                           <  SRR4302545.1272932/94‑1 (MQ=60)
    TCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGG                                                                                           <  SRR4302545.7478964/94‑1 (MQ=60)
     CCTGTTGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGG                                                                                          >  SRR4302545.1294422/1‑94 (MQ=60)
      CTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGA                                                                                         >  SRR4302545.1602828/1‑94 (MQ=60)
       TGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGGT                                                                                        >  SRR4302545.6725058/1‑94 (MQ=60)
       TGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGAT                                                                                        >  SRR4302545.10820308/1‑94 (MQ=60)
        GATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATT                                                                                       >  SRR4302545.2696739/1‑94 (MQ=60)
        GATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATT                                                                                       <  SRR4302545.1675566/94‑1 (MQ=60)
         gtggtTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTG                                                                                      <  SRR4302545.9016045/89‑1 (MQ=60)
         ATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTG                                                                                      <  SRR4302545.304035/94‑1 (MQ=60)
         ATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTG                                                                                      >  SRR4302545.3574499/1‑94 (MQ=60)
          TGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGG                                                                                     <  SRR4302545.9948435/94‑1 (MQ=60)
           GGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGT                                                                                    <  SRR4302545.6426724/94‑1 (MQ=60)
           GGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGT                                                                                    <  SRR4302545.6426723/94‑1 (MQ=60)
           GGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGT                                                                                    >  SRR4302545.1011451/1‑94 (MQ=60)
            GGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTA                                                                                   >  SRR4302545.1128195/1‑94 (MQ=60)
            GGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTA                                                                                   >  SRR4302545.2871175/1‑94 (MQ=60)
             GTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTAT                                                                                  <  SRR4302545.2264470/94‑1 (MQ=60)
             GTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTAT                                                                                  >  SRR4302545.2111422/1‑94 (MQ=60)
             GTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTAT                                                                                  <  SRR4302545.3279417/94‑1 (MQ=60)
               GTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCG                                                                                >  SRR4302545.9438255/1‑94 (MQ=60)
               GTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCG                                                                                <  SRR4302545.7968479/94‑1 (MQ=60)
               GTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCG                                                                                <  SRR4302545.4999432/94‑1 (MQ=60)
               GTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCG                                                                                >  SRR4302545.6632713/1‑94 (MQ=60)
                TGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGG                                                                               <  SRR4302545.7725690/94‑1 (MQ=60)
                  CTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTC                                                                             <  SRR4302545.4323017/94‑1 (MQ=60)
                   TGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCT                                                                            <  SRR4302545.5529609/94‑1 (MQ=60)
                    GTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTG                                                                           <  SRR4302545.10782298/94‑1 (MQ=60)
                    GTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTG                                                                           <  SRR4302545.4199948/94‑1 (MQ=60)
                    GTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTG                                                                           <  SRR4302545.6137602/94‑1 (MQ=60)
                    GTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTG                                                                           >  SRR4302545.4683288/1‑94 (MQ=60)
                    GTTTACGGTAAGTTCTACCACGTGTATCCGTAGCTGGATTTTGCGCAACTAGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTG                                                                           <  SRR4302545.335565/94‑1 (MQ=60)
                     TTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGT                                                                          >  SRR4302545.4732372/1‑94 (MQ=60)
                      TTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTT                                                                         <  SRR4302545.2391863/94‑1 (MQ=60)
                       TACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTC                                                                        <  SRR4302545.9008544/94‑1 (MQ=60)
                       TACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTC                                                                        >  SRR4302545.8934249/1‑94 (MQ=60)
                       TACGGTAATTTCTGCCACGGGGATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGCTTGGGATCGGTGTGTTC                                                                        >  SRR4302545.8131944/1‑94 (MQ=60)
                         CGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCT                                                                      >  SRR4302545.4003910/1‑94 (MQ=60)
                          GGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAATTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTG                                                                     >  SRR4302545.10496036/1‑94 (MQ=60)
                          GGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTGCTG                                                                     >  SRR4302545.9811365/1‑94 (MQ=60)
                          GGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTG                                                                     <  SRR4302545.3363440/94‑1 (MQ=60)
                          GGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTG                                                                     >  SRR4302545.2591233/1‑94 (MQ=60)
                          GGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCACACGGTGTGGCGCAGGGCGCGGGGATTGGTATCGGTCTGTTCCTG                                                                     <  SRR4302545.8166355/94‑1 (MQ=60)
                           GTAATTTCTGCCTCGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGCATCGGTCTGTTCCTGT                                                                    >  SRR4302545.3273108/1‑94 (MQ=60)
                           GTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGC                                                                    >  SRR4302545.6800449/1‑94 (MQ=60)
                           GTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGC                                                                    <  SRR4302545.4208528/94‑1 (MQ=60)
                            TAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCT                                                                   <  SRR4302545.7164580/94‑1 (MQ=60)
                             AATTTCTGCCACGGGTATCCGTAGCTGTATTTTGCGCAACTTGCCGCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTG                                                                  <  SRR4302545.6982729/94‑1 (MQ=60)
                             AATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTG                                                                  >  SRR4302545.1153754/1‑94 (MQ=60)
                                TTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTC                                                               <  SRR4302545.5074623/94‑1 (MQ=60)
                                TTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTC                                                               <  SRR4302545.5697414/94‑1 (MQ=60)
                                 TCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCA                                                              <  SRR4302545.1640614/94‑1 (MQ=60)
                                  ttggCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCAT                                                             <  SRR4302545.8627184/90‑1 (MQ=60)
                                  CTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCAT                                                             <  SRR4302545.8737188/94‑1 (MQ=60)
                                  CTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCAT                                                             >  SRR4302545.5050561/1‑94 (MQ=60)
                                  CTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCAT                                                             >  SRR4302545.2479976/1‑94 (MQ=60)
                                   TGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATT                                                            <  SRR4302545.4856440/94‑1 (MQ=60)
                                   TGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATT                                                            <  SRR4302545.9629414/94‑1 (MQ=60)
                                   TGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATT                                                            >  SRR4302545.1828858/1‑94 (MQ=60)
                                    GCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTG                                                           >  SRR4302545.2546263/1‑94 (MQ=60)
                                    GCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTG                                                           <  SRR4302545.7896480/94‑1 (MQ=60)
                                    GCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTG                                                           <  SRR4302545.4441710/94‑1 (MQ=60)
                                     CCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGCGGATTGGGATCGGTCTGTTCCTGCTGCTCATTGC                                                          >  SRR4302545.7059432/1‑94 (MQ=60)
                                      CACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCC                                                         >  SRR4302545.8705412/1‑94 (MQ=60)
                                       CCGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCG                                                        <  SRR4302545.8051501/94‑1 (MQ=60)
                                       ACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCG                                                        <  SRR4302545.5124638/94‑1 (MQ=60)
                                       ACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCG                                                        <  SRR4302545.6139061/94‑1 (MQ=60)
                                       ACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCG                                                        <  SRR4302545.7674240/94‑1 (MQ=60)
                                        CGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGC                                                       >  SRR4302545.6335657/1‑94 (MQ=60)
                                        CGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGC                                                       >  SRR4302545.813706/1‑94 (MQ=60)
                                         GGGTATCCGTAGCTGGATTTTGCGTATCTTGCCTCACGGTGTGGCGCACGGCTCGGGGATTGGTATCGGTCTGTcgctgttccgcattaccgct                                                      <  SRR4302545.5481006/94‑21 (MQ=60)
                                         GGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCT                                                      <  SRR4302545.539016/94‑1 (MQ=60)
                                         GGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCT                                                      >  SRR4302545.9593705/1‑94 (MQ=60)
                                         GGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCT                                                      <  SRR4302545.9459686/94‑1 (MQ=60)
                                         GGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCT                                                      >  SRR4302545.10115371/1‑94 (MQ=60)
                                         GGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCT                                                      <  SRR4302545.10530676/94‑1 (MQ=60)
                                         CGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCT                                                      <  SRR4302545.8340840/94‑1 (MQ=60)
                                          GGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTA                                                     <  SRR4302545.5986545/94‑1 (MQ=60)
                                          GGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTA                                                     >  SRR4302545.10911598/1‑94 (MQ=60)
                                          GGTATCCGTAGCTGGATTTTGCGCAACTTGACTCACGGTGCGGCGCACGGCGCGGGGATTGGTATCGGTCTGGTCCTACTGCTCATTGCCCCTA                                                     >  SRR4302545.3719155/1‑94 (MQ=60)
                                           GTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAA                                                    >  SRR4302545.1647118/1‑94 (MQ=60)
                                           GTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAA                                                    >  SRR4302545.3876353/1‑94 (MQ=60)
                                           GTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAA                                                    <  SRR4302545.7948984/94‑1 (MQ=60)
                                           GTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAA                                                    >  SRR4302545.7096117/1‑94 (MQ=60)
                                            TATCCGTAGCTGGATTTTGCTCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAAC                                                   <  SRR4302545.8971784/94‑1 (MQ=60)
                                            TATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAAC                                                   >  SRR4302545.29399/1‑94 (MQ=60)
                                            TATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAAC                                                   <  SRR4302545.8560238/94‑1 (MQ=60)
                                            GATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAAC                                                   <  SRR4302545.8384419/94‑1 (MQ=60)
                                             ATCCGTAGCTGGATTTTGCGCAAGTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGATCTGTTCCTGCTGCTCATTGCCGCTAACG                                                  <  SRR4302545.7802918/94‑1 (MQ=60)
                                             ATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACG                                                  >  SRR4302545.6399046/1‑94 (MQ=60)
                                             ATCAGTAGCTGGATTTTGCGCAGCTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCAGTCTGTTCCTGCTGCTCATTGCCGCTAAGG                                                  <  SRR4302545.1548100/94‑1 (MQ=60)
                                               CCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGT                                                <  SRR4302545.7617651/94‑1 (MQ=60)
                                               CCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGT                                                <  SRR4302545.2076947/94‑1 (MQ=60)
                                               CCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGT                                                <  SRR4302545.3531230/94‑1 (MQ=60)
                                                CGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGGTTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTG                                               >  SRR4302545.3850157/1‑94 (MQ=60)
                                                CGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTG                                               >  SRR4302545.7275398/1‑94 (MQ=60)
                                                CGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTG                                               <  SRR4302545.1993387/94‑1 (MQ=60)
                                                CGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTG                                               >  SRR4302545.3893843/1‑94 (MQ=60)
                                                  TAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTC                                             <  SRR4302545.1881516/94‑1 (MQ=60)
                                                   AGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCG                                            >  SRR4302545.6973589/1‑94 (MQ=60)
                                                   AGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCG                                            <  SRR4302545.9027065/94‑1 (MQ=60)
                                                   AGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCG                                            >  SRR4302545.1683057/1‑94 (MQ=60)
                                                   AGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCG                                            >  SRR4302545.4034538/1‑94 (MQ=60)
                                                   AGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGGGTCG                                            >  SRR4302545.7872034/1‑94 (MQ=60)
                                                    GCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGG                                           <  SRR4302545.4062174/94‑1 (MQ=60)
                                                    GCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGG                                           >  SRR4302545.3081550/1‑94 (MQ=60)
                                                    GCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGG                                           >  SRR4302545.3081549/1‑94 (MQ=60)
                                                    GCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGG                                           >  SRR4302545.2666915/1‑94 (MQ=60)
                                                     CTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGT                                          >  SRR4302545.7492281/1‑94 (MQ=60)
                                                     CTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGT                                          >  SRR4302545.6678244/1‑94 (MQ=60)
                                                     CTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGT                                          >  SRR4302545.7492291/1‑94 (MQ=60)
                                                      TGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTC                                         >  SRR4302545.4455654/1‑94 (MQ=60)
                                                      TGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTC                                         >  SRR4302545.8746371/1‑94 (MQ=60)
                                                      TGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTC                                         <  SRR4302545.1489608/94‑1 (MQ=60)
                                                      TGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTC                                         >  SRR4302545.971489/1‑94 (MQ=60)
                                                      TGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTC                                         >  SRR4302545.2500959/1‑94 (MQ=60)
                                                        GATTTTGCGCAACTTGCCTCACGGGGTGGCGCACGGCGCGGGGTCTGGTATCGGCCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGACTG                                       >  SRR4302545.4558461/1‑94 (MQ=60)
                                                         ATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGG                                      <  SRR4302545.7823357/94‑1 (MQ=60)
                                                         ATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGG                                      >  SRR4302545.1982972/1‑94 (MQ=60)
                                                          TTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGT                                     <  SRR4302545.10656713/94‑1 (MQ=60)
                                                          TTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGAGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGT                                     <  SRR4302545.7629204/94‑1 (MQ=60)
                                                          TTTTGCGCAACTTGCCTCACGGCGTGGCGCACGGCGCGGGGATTGGTATCGGTATGTTCCTGCTGCTCATTGACGCTAACTGTATCGGTCTGGG                                     >  SRR4302545.3745221/1‑94 (MQ=60)
                                                           TTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTG                                    >  SRR4302545.4385875/1‑94 (MQ=60)
                                                           TTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTG                                    <  SRR4302545.2431911/94‑1 (MQ=60)
                                                           TTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTG                                    >  SRR4302545.4385874/1‑94 (MQ=60)
                                                           TTTGCGCAACTTGCCTCACGGTGCGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTG                                    >  SRR4302545.4317655/1‑94 (MQ=60)
                                                            TTGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGA                                   <  SRR4302545.472403/94‑1 (MQ=60)
                                                             TGCGCACCTTGCCTCACGGTGTGGCCAACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTACCGGTGTCGGTCTGGTGAT                                  <  SRR4302545.2375887/94‑1 (MQ=60)
                                                             TGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGAT                                  <  SRR4302545.8755629/94‑1 (MQ=60)
                                                             TGCGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGAT                                  <  SRR4302545.9067978/94‑1 (MQ=60)
                                                               CGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTA                                >  SRR4302545.7939589/1‑94 (MQ=60)
                                                               CGCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTA                                <  SRR4302545.11498/94‑1 (MQ=60)
                                                                GCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAA                               >  SRR4302545.809814/1‑94 (MQ=60)
                                                                GCAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAA                               <  SRR4302545.3028574/94‑1 (MQ=60)
                                                                 CAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAA                              >  SRR4302545.10931876/1‑94 (MQ=60)
                                                                 CAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAA                              <  SRR4302545.2413144/94‑1 (MQ=60)
                                                                 CAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAA                              >  SRR4302545.9191829/1‑94 (MQ=60)
                                                                 CAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAA                              >  SRR4302545.9172841/1‑94 (MQ=60)
                                                                 CAACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAA                              <  SRR4302545.3613072/94‑1 (MQ=60)
                                                                  AACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAA                             >  SRR4302545.3923734/1‑94 (MQ=60)
                                                                   ACTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAA                            >  SRR4302545.4567593/1‑94 (MQ=60)
                                                                    CTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAAC                           >  SRR4302545.9933317/1‑94 (MQ=60)
                                                                    CTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAAC                           >  SRR4302545.3029506/1‑94 (MQ=60)
                                                                    CTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAAC                           >  SRR4302545.3814068/1‑94 (MQ=60)
                                                                    CTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAAC                           >  SRR4302545.499406/1‑94 (MQ=60)
                                                                    CTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAAC                           <  SRR4302545.1389679/94‑1 (MQ=60)
                                                                    CTTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAAC                           >  SRR4302545.1370360/1‑94 (MQ=60)
                                                                     TTGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACC                          <  SRR4302545.3058513/94‑1 (MQ=60)
                                                                      TGCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCC                         >  SRR4302545.7055467/1‑94 (MQ=60)
                                                                       GCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCG                        <  SRR4302545.5257481/94‑1 (MQ=60)
                                                                       GCCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCG                        >  SRR4302545.1431826/1‑94 (MQ=60)
                                                                        CCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGC                       <  SRR4302545.5250838/94‑1 (MQ=60)
                                                                        CCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGC                       <  SRR4302545.1766675/94‑1 (MQ=60)
                                                                        CCTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGC                       >  SRR4302545.5464238/1‑94 (MQ=60)
                                                                         CTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCT                      >  SRR4302545.10610004/1‑94 (MQ=60)
                                                                         CTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCT                      >  SRR4302545.4156078/1‑94 (MQ=60)
                                                                         CTCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCT                      >  SRR4302545.7329051/1‑94 (MQ=60)
                                                                          TCACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTT                     <  SRR4302545.2170683/94‑1 (MQ=60)
                                                                           CACGGTGTGGCGGACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTCAAAACCCGCTTG                    >  SRR4302545.2928719/1‑94 (MQ=60)
                                                                           CACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTG                    >  SRR4302545.1263702/1‑94 (MQ=60)
                                                                           CACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTG                    <  SRR4302545.4035397/94‑1 (MQ=60)
                                                                           CACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTG                    <  SRR4302545.8099107/94‑1 (MQ=60)
                                                                           CACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTG                    <  SRR4302545.3560492/94‑1 (MQ=60)
                                                                            ACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGA                   >  SRR4302545.445113/1‑94 (MQ=60)
                                                                            ACGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGA                   >  SRR4302545.1499201/1‑94 (MQ=60)
                                                                             CGGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGAT                  >  SRR4302545.10421599/1‑94 (MQ=60)
                                                                              GGTGTGGCGCACGGCGCGTGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATG                 <  SRR4302545.5661761/94‑1 (MQ=60)
                                                                              GGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATG                 <  SRR4302545.3512342/94‑1 (MQ=60)
                                                                              GGTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATG                 >  SRR4302545.5754231/1‑94 (MQ=60)
                                                                               GTGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGG                >  SRR4302545.7722392/1‑94 (MQ=60)
                                                                                TGTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGT               <  SRR4302545.4518076/94‑1 (MQ=60)
                                                                                 GTGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTC              <  SRR4302545.2805981/94‑1 (MQ=60)
                                                                                  TGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCT             >  SRR4302545.5182848/1‑94 (MQ=60)
                                                                                  TGGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCT             <  SRR4302545.2764646/94‑1 (MQ=60)
                                                                                   GGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTG            <  SRR4302545.1600092/94‑1 (MQ=60)
                                                                                   GGCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTG            <  SRR4302545.7098153/94‑1 (MQ=60)
                                                                                    GCGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGC           <  SRR4302545.9221496/94‑1 (MQ=60)
                                                                                     CGCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCC          >  SRR4302545.9068394/1‑94 (MQ=60)
                                                                                      GCACGTCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCC         <  SRR4302545.4930585/94‑1 (MQ=60)
                                                                                      GCACGGCGCGGGGATTGGTATCGTTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCC         <  SRR4302545.1827711/94‑1 (MQ=60)
                                                                                      GCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCC         >  SRR4302545.5872051/1‑94 (MQ=60)
                                                                                      GCACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCC         <  SRR4302545.6060463/94‑1 (MQ=60)
                                                                                       CACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCG        >  SRR4302545.5619108/1‑94 (MQ=60)
                                                                                       CACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCA        <  SRR4302545.3613851/94‑1 (MQ=60)
                                                                                       CACGGCGCGGGGATTGGTATCGGTCGGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGGGATTAAAAACTCGCTTGCTGGTCTGCCgt        >  SRR4302545.7364272/1‑92 (MQ=60)
                                                                                        ACGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGT       <  SRR4302545.6971126/94‑1 (MQ=60)
                                                                                         CGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTT      >  SRR4302545.6999991/1‑94 (MQ=60)
                                                                                         CGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTT      <  SRR4302545.2352661/94‑1 (MQ=60)
                                                                                         CGGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGCGATTAAAAACCCGCTTGATGGTCTGCCCGTT      >  SRR4302545.2866074/1‑94 (MQ=60)
                                                                                          GGCGCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTG     >  SRR4302545.1259978/1‑94 (MQ=60)
                                                                                             |                                                                                             
GTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGC  >  NC_000913/4276397‑4276583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑