Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,441,820 A→G P44P (CCA→CCG)  ytfN → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,441,8200AG98.9% 1268.0 / 1.0 374P44P (CCA→CCG) ytfNhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base G (180/190);  total (182/192)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-01

Only 100 of 389 total aligned reads displayed.

TTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAGCGG  >  NC_000913/4441729‑4441911
                                                                                           |                                                                                           
ttatcTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCggg                                                                                           <  1:6536077/94‑1 (MQ=255)
ttatcTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCggg                                                                                           >  1:3115800/1‑94 (MQ=255)
 tatcTTTCTGTGGCTGGGAGCGGTGGCTTTCCTGATGGGCCCCACCAGCGGCCTGCATCTGGTCTTTAAAGCGGCGGATCGCTGGGTGCCGGGa                                                                                          <  1:2177494/94‑1 (MQ=255)
 tatcTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCgggg                                                                                          >  1:2839020/1‑93 (MQ=255)
  atcTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGAc                                                                                         >  2:3512523/1‑94 (MQ=255)
  atcTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGAc                                                                                         <  1:1659008/94‑1 (MQ=255)
   tcTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACt                                                                                        >  1:2977251/1‑94 (MQ=255)
    cTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTg                                                                                       >  2:5712514/1‑94 (MQ=255)
       aCTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGat                                                                                    >  2:8638817/1‑94 (MQ=255)
       aCTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGat                                                                                    >  1:5104233/1‑94 (MQ=255)
        cTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGata                                                                                   >  2:9336020/1‑94 (MQ=255)
              cTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCa                                                                             <  1:4270905/94‑1 (MQ=255)
               tGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCaa                                                                            <  2:6613916/94‑1 (MQ=255)
                gggATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAg                                                                           >  2:1531702/1‑94 (MQ=255)
                gggATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAg                                                                           <  1:6435913/94‑1 (MQ=255)
                gggATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAg                                                                           >  2:5904333/1‑94 (MQ=255)
                  gATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAggc                                                                         >  1:5244729/1‑93 (MQ=255)
                    tCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCa                                                                       <  2:7546903/94‑1 (MQ=255)
                    tCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCa                                                                       <  1:10619948/94‑1 (MQ=255)
                     cGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCAc                                                                      >  2:9649917/1‑94 (MQ=255)
                      ggTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCAcc                                                                     >  2:2456169/1‑94 (MQ=255)
                      ggTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCAcc                                                                     <  2:5294885/94‑1 (MQ=255)
                       gTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACcg                                                                    <  2:6045900/94‑1 (MQ=255)
                        tGGCGTGTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACcgg                                                                   <  2:5410714/94‑1 (MQ=255)
                          gCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACcggcg                                                                 <  2:7173299/94‑1 (MQ=255)
                          gCGTTTCGGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACcggcg                                                                 >  1:7561811/1‑94 (MQ=255)
                            gTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCggcggc                                                               >  2:3893177/1‑94 (MQ=255)
                            gTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCggcggc                                                               >  2:10197604/1‑94 (MQ=255)
                              ttCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTg                                                             <  1:9985973/94‑1 (MQ=255)
                                 tggtggGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGgcg                                                          >  2:8866129/1‑94 (MQ=255)
                                  ggtggGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGAGTCGGGACTGGATATTGGCTAGGACACCGGCGGCTGgcgc                                                         >  1:8057404/1‑94 (MQ=255)
                                   gtggGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGcgcg                                                        >  2:1655069/1‑94 (MQ=255)
                                   gtggGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGcgcg                                                        >  2:8283308/1‑94 (MQ=255)
                                     gggCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGAt                                                      <  1:3648893/94‑1 (MQ=255)
                                       gCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGTCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGAtct                                                    <  2:265485/94‑1 (MQ=255)
                                        caccacCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGAtctc                                                   <  2:6223748/94‑1 (MQ=255)
                                        caccacCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGAtctc                                                   >  1:416673/1‑94 (MQ=255)
                                         accaccAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCa                                                  >  2:3380706/1‑94 (MQ=255)
                                         accaccAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCa                                                  >  2:4584025/1‑94 (MQ=255)
                                          ccaccaGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCAc                                                 <  2:10434561/94‑1 (MQ=255)
                                            accaGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCt                                               <  1:8428270/94‑1 (MQ=255)
                                            accaGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCt                                               >  1:1882516/1‑94 (MQ=255)
                                             ccaGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCtt                                              <  2:8109313/94‑1 (MQ=255)
                                             ccaGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCtt                                              <  1:4877381/94‑1 (MQ=255)
                                              caGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTg                                             <  2:4781456/94‑1 (MQ=255)
                                              caGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTg                                             >  1:881743/1‑94 (MQ=255)
                                               aGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGt                                            >  1:956622/1‑94 (MQ=255)
                                                gCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTc                                           <  2:6776231/94‑1 (MQ=255)
                                                 cGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCCCCCTGTCt                                          >  2:10270952/1‑94 (MQ=255)
                                                 cGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCt                                          >  1:5386365/1‑94 (MQ=255)
                                                  ggCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGCCTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTg                                         >  2:3696166/1‑94 (MQ=255)
                                                   gCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGa                                        <  2:4928210/94‑1 (MQ=255)
                                                    ccTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGAc                                       <  1:5279193/94‑1 (MQ=255)
                                                    ccTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGAc                                       <  2:2109124/94‑1 (MQ=255)
                                                    ccTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGAc                                       >  1:9785515/1‑94 (MQ=255)
                                                    ccTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGAc                                       >  2:6447622/1‑94 (MQ=255)
                                                     cTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGAcg                                      <  2:1091162/94‑1 (MQ=255)
                                                     cTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGAcg                                      >  1:7043615/1‑94 (MQ=255)
                                                      tGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGAcgt                                     >  1:2776978/1‑94 (MQ=255)
                                                        cATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGAcgttc                                   <  1:2597520/94‑1 (MQ=255)
                                                         aTCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGAcgttcg                                  >  2:224738/1‑94 (MQ=255)
                                                            tGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTa                               >  2:9828704/1‑94 (MQ=255)
                                                            tGGTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGGTCGTTa                               >  1:4746532/1‑94 (MQ=255)
                                                             tgTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTAt                              <  2:8705484/93‑1 (MQ=255)
                                                             ggTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTAt                              <  2:4077363/94‑1 (MQ=255)
                                                             ggTATTTAAAGAGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGTTGGCGCGATCTCACCTTGTCTGACGTTCGTTAt                              <  1:3314436/94‑1 (MQ=255)
                                                              gTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATg                             <  1:5225167/94‑1 (MQ=255)
                                                              gTATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATg                             <  1:5569074/94‑1 (MQ=255)
                                                               tATTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATgg                            >  2:8423365/1‑93 (MQ=255)
                                                                aTTTAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGag                           <  1:660341/94‑1 (MQ=255)
                                                                  ttAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGagca                         <  2:5138538/94‑1 (MQ=255)
                                                                  ttAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGagca                         <  1:2056348/94‑1 (MQ=255)
                                                                   tAAAGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGagcag                        <  1:5015867/94‑1 (MQ=255)
                                                                    aaaGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGagcagc                       >  1:5787970/1‑94 (MQ=255)
                                                                    aaaGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGagcagc                       <  1:6775214/94‑1 (MQ=255)
                                                                    aaaGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGagcagc                       >  1:7742670/1‑94 (MQ=255)
                                                                      aGCGGCGGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGGCGTTCGTTATGAGCAGCCa                     >  2:7839509/1‑94 (MQ=255)
                                                                       gcggcgGATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAg                    <  2:1817182/94‑1 (MQ=255)
                                                                        cggcggATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAgg                   >  1:6011959/1‑94 (MQ=255)
                                                                        cggcggATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAgg                   <  2:2784049/94‑1 (MQ=255)
                                                                        cggcggATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCAATCTGACCTTGTCTGACGTTCGTGTTGAGCGGCCCgg                   >  1:2318867/1‑94 (MQ=255)
                                                                         ggcggATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGc                  <  2:9137167/94‑1 (MQ=255)
                                                                           cggATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCgg                >  1:9046763/1‑93 (MQ=255)
                                                                            ggATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGGGCAGCCAGGCGTg               <  1:869719/94‑1 (MQ=255)
                                                                            ggATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTg               >  1:2453213/1‑94 (MQ=255)
                                                                             gATCGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTgg              <  1:141302/94‑1 (MQ=255)
                                                                             gATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTgg              <  2:4390030/94‑1 (MQ=255)
                                                                                cGCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCgg           <  2:4298042/94‑1 (MQ=255)
                                                                                 gCTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGt          >  1:4419877/1‑94 (MQ=255)
                                                                                  cTGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTa         >  1:1775981/1‑94 (MQ=255)
                                                                                  cTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTTGCGGTa         >  1:10007772/1‑94 (MQ=255)
                                                                                   tGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTaa        <  1:4694814/94‑1 (MQ=255)
                                                                                   tGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTaa        >  1:6280992/1‑94 (MQ=255)
                                                                                   tGGGTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTaa        <  1:1187876/94‑1 (MQ=255)
                                                                                    gggTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTaaa       >  1:3565375/1‑94 (MQ=255)
                                                                                    gggTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTaaa       <  2:9571629/94‑1 (MQ=255)
                                                                                     ggTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTaaaa      >  2:3027984/1‑94 (MQ=255)
                                                                                      gTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAg     <  1:37603/94‑1 (MQ=255)
                                                                                      gTGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAg     <  1:101668/94‑1 (MQ=255)
                                                                                       tGCCGGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAGc    <  2:125888/94‑1 (MQ=255)
                                                                                           |                                                                                           
TTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAGCGG  >  NC_000913/4441729‑4441911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 220 total aligned reads displayed.
GATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CG‑‑GCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCAT  >  NC_000913/4448448‑4448634
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GATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CG                                                                                                       <  SRR4302545.2175998/94‑1 (MQ=60)
 ATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CG‑‑C                                                                                                    >  SRR4302545.1730932/1‑94 (MQ=60)
 ATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CG‑‑C                                                                                                    >  SRR4302545.5143120/1‑94 (MQ=60)
 ATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CG‑‑C                                                                                                    >  SRR4302545.5143123/1‑94 (MQ=60)
 ATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CG‑‑C                                                                                                    <  SRR4302545.8897620/94‑1 (MQ=60)
  TACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CGcg                                                                                                     <  SRR4302545.793775/94‑3 (MQ=60)
  TACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CGcg                                                                                                     <  SRR4302545.298610/94‑3 (MQ=60)
  TACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CGcg                                                                                                     >  SRR4302545.6875336/1‑92 (MQ=60)
  TACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CGcg                                                                                                     >  SRR4302545.7043481/1‑92 (MQ=60)
   ACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑C‑‑‑GCG‑‑‑C                                                                                              >  SRR4302545.9463265/1‑94 (MQ=60)
   ACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑C‑‑‑GCG‑‑‑C                                                                                              <  SRR4302545.6716079/94‑1 (MQ=60)
   ACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑C‑‑‑GCG‑‑‑C                                                                                              >  SRR4302545.9574392/1‑94 (MQ=60)
   ACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑C‑‑‑GCG‑‑‑C                                                                                              >  SRR4302545.9574398/1‑94 (MQ=60)
   ACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑C‑‑‑GCG‑‑‑C                                                                                              >  SRR4302545.710028/1‑94 (MQ=60)
    CCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGTCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CGcgcc                                                                                                   >  SRR4302545.5216825/1‑90 (MQ=60)
    CCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CGcgcc                                                                                                   >  SRR4302545.9649680/1‑90 (MQ=60)
     CGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CGcgccg                                                                                                  >  SRR4302545.696977/1‑89 (MQ=60)
     CGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CGcgccg                                                                                                  <  SRR4302545.6374344/94‑6 (MQ=60)
     CGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CGcgccg                                                                                                  >  SRR4302545.7143010/1‑89 (MQ=60)
      GCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CGcgccgc                                                                                                 >  SRR4302545.5307459/1‑88 (MQ=60)
       CAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CGcgccgca                                                                                                >  SRR4302545.8835194/1‑87 (MQ=60)
       CAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CGcgccgca                                                                                                >  SRR4302545.1789766/1‑87 (MQ=60)
        AAAAGAAGAATTTGAGTAGAAGAAAAATATGGGGTATTGAGGGGTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGA‑‑GCT‑‑CGcggctgct                                                                                               >  SRR4302545.1489669/1‑86 (MQ=60)
        AAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CGcgccgcat                                                                                               <  SRR4302545.4195885/94‑9 (MQ=60)
        AAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CGcgccgcat                                                                                               <  SRR4302545.9408468/94‑9 (MQ=60)
        AAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CGcgccgcat                                                                                               <  SRR4302545.9802864/94‑9 (MQ=60)
         AAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CGcgccgcatc                                                                                              >  SRR4302545.8484694/1‑85 (MQ=60)
          AAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CGcgccgcatcc                                                                                             <  SRR4302545.4346903/94‑11 (MQ=60)
          AAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CGcgccgcatcc                                                                                             >  SRR4302545.1277322/1‑84 (MQ=60)
          AAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CGcgccgcatcc                                                                                             <  SRR4302545.6254504/94‑11 (MQ=60)
           AGAAGAATTAGAGAAGAAGAAATATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCG                                                                                      <  SRR4302545.3636614/94‑1 (MQ=60)
           AGAAGAATTAGAGAAGAAGAAAAATCTGGGGTATTGAGGGTTGCTATGCATGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCG                                                                                      >  SRR4302545.5286495/1‑94 (MQ=60)
            GAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGG                                                                                     <  SRR4302545.8038777/94‑1 (MQ=60)
            GAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGG                                                                                     >  SRR4302545.5859770/1‑94 (MQ=60)
             AAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CGcgccgcctccggc                                                                                          >  SRR4302545.6400420/1‑81 (MQ=60)
              AGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCG                                                                                   <  SRR4302545.3370107/94‑1 (MQ=60)
              AGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCG                                                                                   >  SRR4302545.4028957/1‑94 (MQ=60)
               TAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGA                                                                                  >  SRR4302545.2045844/1‑94 (MQ=60)
                AATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGAT                                                                                 >  SRR4302545.492639/1‑94 (MQ=60)
                AATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGAT                                                                                 >  SRR4302545.3734120/1‑94 (MQ=60)
                AATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGAT                                                                                 >  SRR4302545.3734121/1‑94 (MQ=60)
                 ATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATG                                                                                >  SRR4302545.3297415/1‑94 (MQ=60)
                   TAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGT                                                                              >  SRR4302545.7167320/1‑94 (MQ=60)
                   TAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGT                                                                              >  SRR4302545.9502332/1‑94 (MQ=60)
                   TAGAGAAGAAGAAAAATATGGGGTATGGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑C‑‑‑GCG‑‑‑CCGAATCCGGCGATGGT                                                                              <  SRR4302545.6704959/94‑1 (MQ=60)
                    AGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTG                                                                             >  SRR4302545.3217124/1‑94 (MQ=60)
                    AGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTG                                                                             >  SRR4302545.110716/1‑94 (MQ=60)
                    AGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTG                                                                             >  SRR4302545.7195060/1‑94 (MQ=60)
                     GAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGC                                                                            >  SRR4302545.10421585/1‑94 (MQ=60)
                     GAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGA                                                                            >  SRR4302545.9070342/1‑94 (MQ=60)
                      AGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCA                                                                           >  SRR4302545.9806633/1‑94 (MQ=60)
                      AGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCA                                                                           >  SRR4302545.2313109/1‑94 (MQ=60)
                      AGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCA                                                                           >  SRR4302545.2008597/1‑94 (MQ=60)
                      AGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCA                                                                           >  SRR4302545.8226375/1‑94 (MQ=60)
                       GAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCAC                                                                          >  SRR4302545.8826331/1‑94 (MQ=60)
                       GAAGAAAAAAAATAGGGGGTATTGAGGGTTGCTATGCCGGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCAC                                                                          <  SRR4302545.7945565/94‑1 (MQ=56)
                        AAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACT                                                                         >  SRR4302545.4875142/1‑94 (MQ=60)
                        AAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACT                                                                         >  SRR4302545.7555942/1‑94 (MQ=60)
                        AAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑AGCCGGCGATGGTGCACT                                                                         <  SRR4302545.1053419/94‑1 (MQ=60)
                          GAAGAAAAATATGGGGTATTGAGGTTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGA                                                                       <  SRR4302545.1946109/94‑1 (MQ=60)
                           AAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAA                                                                      <  SRR4302545.3400458/94‑1 (MQ=60)
                           AAAAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAA                                                                      <  SRR4302545.6059158/94‑1 (MQ=60)
                           AAAAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAA                                                                      <  SRR4302545.2240838/94‑1 (MQ=60)
                            AGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAG                                                                     <  SRR4302545.6623810/94‑1 (MQ=60)
                            AGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAG                                                                     <  SRR4302545.1133714/94‑1 (MQ=60)
                               AAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCT                                                                  >  SRR4302545.8263130/1‑94 (MQ=60)
                               AAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCT                                                                  <  SRR4302545.5755106/94‑1 (MQ=60)
                               AAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCT                                                                  >  SRR4302545.2096295/1‑94 (MQ=60)
                               AAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCT                                                                  >  SRR4302545.2043623/1‑94 (MQ=60)
                               AAAAAAGGGGGTATTGAGGTTTCCTAGGCCTGATGCCGATTCGTAGGCCGGAAAAGGCGTGCG‑‑CG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCT                                                                  <  SRR4302545.2599686/94‑1 (MQ=48)
                                AAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTG                                                                 <  SRR4302545.6379418/94‑1 (MQ=60)
                                 AATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGA                                                                >  SRR4302545.6867258/1‑94 (MQ=60)
                                  ATATGGGGTATTGGGGTTTGCTATGCCTGATGCCGTTTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGAT                                                               <  SRR4302545.2014975/94‑1 (MQ=56)
                                  ATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑GTCCAGCGATGGTGCACTGAAGCCTGAT                                                               >  SRR4302545.5959896/1‑94 (MQ=60)
                                  ATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGGACTGAAGACTGAT                                                               >  SRR4302545.2213811/1‑94 (MQ=60)
                                  ATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGAT                                                               <  SRR4302545.697205/94‑1 (MQ=60)
                                  ATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGAT                                                               <  SRR4302545.343186/94‑1 (MQ=60)
                                  ATATGGGGTATGGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCGGGATAGGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGAT                                                               <  SRR4302545.2301386/94‑1 (MQ=60)
                                  ATATGGGGCATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGcg                                                               >  SRR4302545.6328724/1‑92 (MQ=60)
                                   TATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATG                                                              >  SRR4302545.577071/1‑94 (MQ=60)
                                   TATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGCCGATGGTGCACTGAAGCCTGATG                                                              <  SRR4302545.6908654/94‑1 (MQ=60)
                                   TATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CGCCCCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATG                                                              <  SRR4302545.6536725/94‑1 (MQ=60)
                                    ATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGC                                                             <  SRR4302545.4902870/94‑1 (MQ=60)
                                    ATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGC                                                             >  SRR4302545.9344171/1‑94 (MQ=60)
                                    ATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGC                                                             >  SRR4302545.5582515/1‑94 (MQ=60)
                                    ATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGC                                                             <  SRR4302545.1445820/94‑1 (MQ=60)
                                     TGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCG                                                            >  SRR4302545.8511673/1‑94 (MQ=60)
                                     TGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCG                                                            >  SRR4302545.7742624/1‑94 (MQ=60)
                                     TGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCG                                                            <  SRR4302545.124037/94‑1 (MQ=60)
                                     TGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCG                                                            >  SRR4302545.3654659/1‑94 (MQ=60)
                                       GGGTATTGAGGGTTGCTATGCCTGCTGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCCCG‑‑CCT‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGctgggat                                                          >  SRR4302545.4626548/1‑87 (MQ=60)
                                       GGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGAC                                                          <  SRR4302545.8865584/94‑1 (MQ=60)
                                       GGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGAC                                                          <  SRR4302545.5377048/94‑1 (MQ=60)
                                        GGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCAGTGAAGGCTGAAGCGCCG                                                         <  SRR4302545.6650588/94‑1 (MQ=51)
                                        GGTATGGAGGTTTCCTATGCTTGAGGCCGATTCGTGGGCGGGATAGGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCGGGCGATGGTGCACTGAAGCCTGATGCGACG                                                         <  SRR4302545.9636336/94‑1 (MQ=25)
                                          TATTGAGGGTTGCTCTGCCTGATGCAGCTTCGTAGGCCGGATAAGGA‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAAGCTGATGAGACGCT                                                       >  SRR4302545.7634163/1‑94 (MQ=22)
                                          TATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGGCGCT                                                       >  SRR4302545.7675212/1‑94 (MQ=60)
                                          TATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCT                                                       <  SRR4302545.1698761/94‑1 (MQ=60)
                                          TATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCT                                                       >  SRR4302545.5490141/1‑94 (MQ=60)
                                          TATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCT                                                       >  SRR4302545.8953183/1‑94 (MQ=60)
                                          TATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCT                                                       >  SRR4302545.8507346/1‑94 (MQ=60)
                                          TATTGAAGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCT                                                       >  SRR4302545.10834187/1‑94 (MQ=60)
                                           ATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTT                                                      <  SRR4302545.9139369/94‑1 (MQ=60)
                                           ATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTT                                                      <  SRR4302545.4904910/94‑1 (MQ=60)
                                           ATTGAGGGTTGCTATGCCGTATGCCGATTCGTAGGCCGGATAAGGC‑‑CCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTT                                                      <  SRR4302545.2294762/94‑1 (MQ=60)
                                            TTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTA                                                     >  SRR4302545.8431537/1‑94 (MQ=60)
                                            TTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTA                                                     >  SRR4302545.4904859/1‑94 (MQ=60)
                                            TTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTA                                                     <  SRR4302545.6960264/94‑1 (MQ=60)
                                            TTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTA                                                     <  SRR4302545.1925935/94‑1 (MQ=60)
                                             TGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAC                                                    >  SRR4302545.8522251/1‑94 (MQ=60)
                                             TGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAC                                                    <  SRR4302545.10030887/94‑1 (MQ=60)
                                              GAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACC                                                   >  SRR4302545.3422269/1‑94 (MQ=60)
                                               GGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑C‑‑‑GCGCCTC‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCG                                                  <  SRR4302545.225676/94‑1 (MQ=60)
                                               AGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCG                                                  >  SRR4302545.8428346/1‑94 (MQ=60)
                                               AGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATCGTGCACTGAAGCCTGATGCGACGCTTACCG                                                  >  SRR4302545.5935137/1‑94 (MQ=60)
                                                 GGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCG                                                <  SRR4302545.5593739/94‑1 (MQ=60)
                                                  ATTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGGCTGATGCGACGCATACCGCGT                                               >  SRR4302545.190470/1‑94 (MQ=60)
                                                    TGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCT                                             <  SRR4302545.2235617/94‑1 (MQ=60)
                                                      TTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTA                                           <  SRR4302545.6782680/94‑1 (MQ=60)
                                                      CTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTA                                           <  SRR4302545.647656/94‑1 (MQ=60)
                                                      CTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAACGCGTCTTA                                           >  SRR4302545.5881019/1‑94 (MQ=60)
                                                       TATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTAT                                          >  SRR4302545.8752405/1‑94 (MQ=60)
                                                       TATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTAT                                          <  SRR4302545.10818587/94‑1 (MQ=60)
                                                        ATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATC                                         <  SRR4302545.5268224/94‑1 (MQ=60)
                                                         TGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCA                                        >  SRR4302545.7901796/1‑94 (MQ=60)
                                                         TGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCA                                        <  SRR4302545.1243069/94‑1 (MQ=60)
                                                          GCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCAT                                       >  SRR4302545.10221364/1‑94 (MQ=60)
                                                           cgtgatcctGATTCGTAGGCCGGAAAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATG                                      <  SRR4302545.309903/85‑1 (MQ=60)
                                                           CCTGATGCCGATTCGTAGGCGGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATG                                      <  SRR4302545.2146202/94‑1 (MQ=60)
                                                           CCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTTTTATCATG                                      >  SRR4302545.10006616/1‑94 (MQ=60)
                                                            CTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGC                                     >  SRR4302545.10105767/1‑94 (MQ=60)
                                                            CTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGC                                     >  SRR4302545.10939071/1‑94 (MQ=60)
                                                             TGATGCCGATTCGTAGGCCGGATAGGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCC                                    <  SRR4302545.4504292/94‑1 (MQ=60)
                                                             TGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCC                                    <  SRR4302545.8902374/94‑1 (MQ=60)
                                                             TGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCC                                    <  SRR4302545.280607/94‑1 (MQ=60)
                                                              GATGCGGATTCGTAGGCCCGATAAGCC‑‑CCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCT                                   <  SRR4302545.1234218/94‑1 (MQ=56)
                                                              GAGGCCGATGCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCT                                   <  SRR4302545.6185143/94‑1 (MQ=60)
                                                               ATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTA                                  <  SRR4302545.7132718/94‑1 (MQ=60)
                                                               ATGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTA                                  >  SRR4302545.8855948/1‑94 (MQ=60)
                                                                ggccaATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTAC                                 <  SRR4302545.9776727/89‑1 (MQ=60)
                                                                TGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTAC                                 <  SRR4302545.907615/94‑1 (MQ=60)
                                                                TGCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTAC                                 <  SRR4302545.3820098/94‑1 (MQ=60)
                                                                 GCCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACT                                >  SRR4302545.8897366/1‑94 (MQ=60)
                                                                  CCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTG                               >  SRR4302545.6685342/1‑94 (MQ=60)
                                                                  CCGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTG                               <  SRR4302545.10387606/94‑1 (MQ=60)
                                                                   CGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGG                              >  SRR4302545.7289583/1‑94 (MQ=60)
                                                                   CGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGG                              >  SRR4302545.7637397/1‑94 (MQ=60)
                                                                   CGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGG                              >  SRR4302545.4975101/1‑94 (MQ=60)
                                                                   CGATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGG                              >  SRR4302545.1057098/1‑94 (MQ=60)
                                                                    GATTCGTAGGCCGGATAGGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ACCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGG                             <  SRR4302545.4608401/94‑1 (MQ=60)
                                                                    GATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGG                             >  SRR4302545.7719034/1‑94 (MQ=60)
                                                                    GATTCGTAGGCCGGATAAGGC‑‑GC‑‑‑CC‑‑GCGCCCC‑‑‑ATCGGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGG                             <  SRR4302545.9768014/94‑1 (MQ=60)
                                                                    GATCCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGG                             <  SRR4302545.6548795/94‑1 (MQ=60)
                                                                     ATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGA                            <  SRR4302545.5770349/94‑1 (MQ=60)
                                                                     ATTCGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGGGCACTGAAGCCTGATGCGACGCTTACCGCGGCTTATCATGCCTCCTGGGG                            >  SRR4302545.7955584/1‑94 (MQ=54)
                                                                       TCGGAGGCCGGATAAGGC‑‑GCTCCCC‑‑CCG‑‑‑A‑‑‑ATCCGTCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGC                          <  SRR4302545.6360581/94‑1 (MQ=54)
                                                                        CGTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCA                         >  SRR4302545.10845496/1‑94 (MQ=60)
                                                                         GTAGGCGGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCAC                        <  SRR4302545.4560978/94‑1 (MQ=60)
                                                                         GTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCAC                        <  SRR4302545.5480623/94‑1 (MQ=60)
                                                                         GTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCAC                        >  SRR4302545.6543552/1‑94 (MQ=60)
                                                                         GTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCAC                        <  SRR4302545.2826706/94‑1 (MQ=60)
                                                                         GTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCAC                        >  SRR4302545.7659245/1‑94 (MQ=60)
                                                                         GTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTCAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCAC                        >  SRR4302545.9598093/1‑94 (MQ=60)
                                                                         GTAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCAGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCAC                        >  SRR4302545.4166059/1‑94 (MQ=60)
                                                                          GAGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACG                       <  SRR4302545.9294207/94‑1 (MQ=60)
                                                                           AGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGC                      >  SRR4302545.9204150/1‑94 (MQ=60)
                                                                           AGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGC                      >  SRR4302545.8063312/1‑94 (MQ=60)
                                                                           AGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGC                      >  SRR4302545.1684921/1‑94 (MQ=60)
                                                                           AGGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGCGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGC                      >  SRR4302545.8063314/1‑94 (MQ=60)
                                                                            GGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCT                     >  SRR4302545.6049378/1‑94 (MQ=60)
                                                                            GGCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACAGGGAGCACGCT                     >  SRR4302545.3182978/1‑94 (MQ=60)
                                                                             GCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTT                    >  SRR4302545.1075053/1‑94 (MQ=60)
                                                                             GCCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTAGGAGCACGCTT                    >  SRR4302545.5432064/1‑94 (MQ=60)
                                                                              CCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTT                   <  SRR4302545.3659897/94‑1 (MQ=60)
                                                                              CCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTT                   >  SRR4302545.2969008/1‑94 (MQ=60)
                                                                              CCGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTT                   <  SRR4302545.3302358/94‑1 (MQ=60)
                                                                               CGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTA                  >  SRR4302545.8640414/1‑94 (MQ=60)
                                                                               CGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTA                  >  SRR4302545.8433728/1‑94 (MQ=60)
                                                                               CGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTA                  >  SRR4302545.10385169/1‑94 (MQ=60)
                                                                               CGGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTA                  >  SRR4302545.4999507/1‑94 (MQ=60)
                                                                                GGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTAC                 <  SRR4302545.5410840/94‑1 (MQ=60)
                                                                                GGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTAC                 >  SRR4302545.6968243/1‑94 (MQ=60)
                                                                                GGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTAC                 >  SRR4302545.4888448/1‑94 (MQ=60)
                                                                                GGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTAC                 <  SRR4302545.8343370/94‑1 (MQ=60)
                                                                                GGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTAC                 >  SRR4302545.10939008/1‑94 (MQ=60)
                                                                                GGATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTAC                 <  SRR4302545.8343377/94‑1 (MQ=60)
                                                                                 GATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACA                >  SRR4302545.1705244/1‑94 (MQ=60)
                                                                                 GATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACA                <  SRR4302545.9035534/94‑1 (MQ=60)
                                                                                 GATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACA                <  SRR4302545.3454243/94‑1 (MQ=60)
                                                                                  ATAAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACAC               <  SRR4302545.2217358/94‑1 (MQ=60)
                                                                                    AAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCG             >  SRR4302545.10542294/1‑94 (MQ=60)
                                                                                    AAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCG             >  SRR4302545.9608205/1‑94 (MQ=60)
                                                                                    AAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCG             >  SRR4302545.3244618/1‑94 (MQ=60)
                                                                                    AAGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCG             <  SRR4302545.5788137/94‑1 (MQ=60)
                                                                                     AGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGG            <  SRR4302545.1181641/94‑1 (MQ=60)
                                                                                     AGGC‑‑GCTCGCG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGG            <  SRR4302545.7155131/94‑1 (MQ=60)
                                                                                                CG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCA   >  SRR4302545.10745754/1‑94 (MQ=60)
                                                                                                CG‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCA   >  SRR4302545.8890309/1‑94 (MQ=60)
                                                                                                 G‑‑CCG‑‑‑C‑‑‑ATCGGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCATGCTTTACACCGGGGGAAACCAT  <  SRR4302545.7865310/94‑1 (MQ=60)
                                                                                                 G‑‑CCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCAT  <  SRR4302545.6508222/94‑1 (MQ=60)
                                                                                                 |                                                                                                     
GATACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGC‑‑GCT‑‑CG‑‑GCG‑‑‑C‑‑‑ATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCAT  >  NC_000913/4448448‑4448634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑