Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,500,500 A→G V167A (GTC→GCC)  yi41 ← IS4 hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,500,5000AG99.1% 1548.6 / ‑0.9 458V167A (GTC→GCC) yi41IS4 hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base G (226/228);  total (228/230)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

Only 100 of 471 total aligned reads displayed.

TATAAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGACCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGTGTGCGCC  >  NC_000913/4500409‑4500590
                                                                                           |                                                                                          
tataAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGcc                                                                                          >  1:6673978/1‑94 (MQ=255)
tataAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGcc                                                                                          <  2:8996301/94‑1 (MQ=255)
tataAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGcc                                                                                          >  2:7555732/1‑94 (MQ=255)
  taAGTCGCTCAGCAAGCTCATTTTCGCTGTTCTTAATCGTACCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCCTCTGGCAGGCCAt                                                                                        <  1:1224445/94‑1 (MQ=255)
   aaGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCAtt                                                                                       >  2:4552901/1‑94 (MQ=255)
   aaGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCAtt                                                                                       >  1:7518605/1‑94 (MQ=255)
   aaGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCAtt                                                                                       <  1:5595267/94‑1 (MQ=255)
     gTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCAtttt                                                                                     >  2:1219326/1‑94 (MQ=255)
     gTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCAtttt                                                                                     <  1:458687/94‑1 (MQ=255)
     gTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCAtttt                                                                                     <  2:4779367/94‑1 (MQ=255)
      tggCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTg                                                                                    <  1:5766321/92‑1 (MQ=255)
       tGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGa                                                                                   <  1:6128233/94‑1 (MQ=255)
          tCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCt                                                                                >  2:2715619/1‑94 (MQ=255)
           cAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTg                                                                               <  2:5556483/94‑1 (MQ=255)
            aGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGc                                                                              >  1:7713052/1‑94 (MQ=255)
              cAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCgg                                                                            >  1:7971965/1‑94 (MQ=255)
              cAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCgg                                                                            <  2:9448588/94‑1 (MQ=255)
              cAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCgg                                                                            >  2:195368/1‑94 (MQ=255)
              cAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCgg                                                                            >  2:9294114/1‑94 (MQ=255)
              cAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGACAGGCCATTTTGACCTGCgg                                                                            >  1:9041058/1‑94 (MQ=255)
               aaGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCACCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCggg                                                                           >  1:1338008/1‑94 (MQ=255)
                 gCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTa                                                                         >  1:9428834/1‑94 (MQ=255)
                  cTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTag                                                                        <  1:3361314/94‑1 (MQ=255)
                    cATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTagag                                                                      <  1:3968262/94‑1 (MQ=255)
                    cATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTagag                                                                      <  1:4845703/94‑1 (MQ=255)
                     aTTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAgc                                                                     <  2:7708352/94‑1 (MQ=255)
                     aTTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAgc                                                                     >  2:1738690/1‑94 (MQ=255)
                     aTTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAgc                                                                     <  2:8315562/94‑1 (MQ=255)
                      ttttCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGGTGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAgcg                                                                    <  2:8769862/94‑1 (MQ=255)
                        ttCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGcc                                                                  <  1:8729639/94‑1 (MQ=255)
                        ttCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGcc                                                                  <  2:2915086/94‑1 (MQ=255)
                         tCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCg                                                                 >  1:2995840/1‑94 (MQ=255)
                         tCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCg                                                                 <  1:5023816/94‑1 (MQ=255)
                          cGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCgg                                                                <  2:5109685/94‑1 (MQ=255)
                          cGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCgg                                                                >  1:6463005/1‑94 (MQ=255)
                          cGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCgg                                                                >  2:7127419/1‑94 (MQ=255)
                           gCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCggg                                                               <  2:3771577/94‑1 (MQ=255)
                           gCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCggg                                                               >  2:1551734/1‑94 (MQ=255)
                              tttcttcATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTc                                                            <  2:7294874/93‑1 (MQ=255)
                              gTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTc                                                            >  1:6045055/1‑94 (MQ=255)
                              gTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTc                                                            <  2:2104895/94‑1 (MQ=255)
                              gTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTc                                                            >  1:8315235/1‑94 (MQ=255)
                               ttcttcATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTcc                                                           >  2:6977958/1‑94 (MQ=255)
                               ttcttcATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTcc                                                           >  2:4885555/1‑94 (MQ=255)
                                tcttcATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTccc                                                          >  2:3618166/1‑94 (MQ=255)
                                tcttcATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGACCATTTTGACCTGCGGGTAGAGCGCCGGGTTccc                                                          >  1:3679550/1‑94 (MQ=255)
                                 cttcATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCg                                                         <  2:1022747/94‑1 (MQ=255)
                                  ttcATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCgg                                                        <  2:8420787/94‑1 (MQ=255)
                                   tcATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGc                                                       >  1:4187739/1‑94 (MQ=255)
                                     aTCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCAt                                                     >  2:4320978/1‑94 (MQ=255)
                                      tCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCAtg                                                    <  1:629522/94‑1 (MQ=255)
                                       cGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCAtgt                                                   <  1:10221235/94‑1 (MQ=255)
                                        gTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCAtgtg                                                  >  2:6416819/1‑94 (MQ=255)
                                          gCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTc                                                <  1:8148868/94‑1 (MQ=255)
                                            cGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCCTGTGTTTg                                              >  2:8199058/1‑94 (MQ=255)
                                            cGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTg                                              <  1:1659621/94‑1 (MQ=255)
                                            cGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTg                                              <  2:6723390/94‑1 (MQ=255)
                                             gAAGGCTGCCGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCGCGGCATGTGTCTgg                                             >  2:8512649/1‑94 (MQ=255)
                                             gAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTgg                                             <  1:230584/94‑1 (MQ=255)
                                             gAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGAAGGCCATTTTGCCCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTgg                                             <  2:5181918/94‑1 (MQ=255)
                                              aaGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCGTGTGTCTGGc                                            >  2:6161131/1‑94 (MQ=255)
                                               aGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCg                                           <  2:2383290/94‑1 (MQ=255)
                                               aGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCg                                           >  1:6946580/1‑94 (MQ=255)
                                                 gCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCggg                                         >  1:2638400/1‑94 (MQ=255)
                                                 gCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCggg                                         <  1:7094448/94‑1 (MQ=255)
                                                 gCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCggg                                         <  1:9899873/94‑1 (MQ=255)
                                                  cTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCgggg                                        <  2:801914/94‑1 (MQ=255)
                                                  cTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCgggg                                        <  2:7809975/94‑1 (MQ=255)
                                                   tGCAGCCGTCAGCAGAGGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCggggg                                       <  1:3839062/94‑1 (MQ=255)
                                                      aGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAg                                    <  1:5928641/94‑1 (MQ=255)
                                                        ccGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGc                                  <  2:9705389/94‑1 (MQ=255)
                                                         cGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCt                                 <  1:2422771/94‑1 (MQ=255)
                                                         cGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCt                                 <  1:6869772/94‑1 (MQ=255)
                                                          gTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTg                                >  1:10499568/1‑94 (MQ=255)
                                                           tCAGCAGATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGc                               >  1:4486278/1‑94 (MQ=255)
                                                            cagcagATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCa                              <  1:3280735/94‑1 (MQ=255)
                                                             agcagATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCAt                             <  2:5729802/94‑1 (MQ=255)
                                                               cagATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCg                           >  1:2134404/1‑94 (MQ=255)
                                                               cagATGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCg                           >  1:528613/1‑94 (MQ=255)
                                                               cagATGGCTGGTAAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCTCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCg                           <  2:4056232/94‑1 (MQ=255)
                                                                   tGGCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTtct                       <  2:3280647/94‑1 (MQ=255)
                                                                     gCTGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTctct                     <  1:6622758/94‑1 (MQ=255)
                                                                       tGGTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTgg                   >  1:10031208/1‑94 (MQ=255)
                                                                        ggTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGgt                  <  2:10619924/94‑1 (MQ=255)
                                                                        ggTCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGgt                  <  2:5391107/94‑1 (MQ=255)
                                                                          tCAGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGgtgt                >  1:10138887/1‑94 (MQ=255)
                                                                            aGTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTAt              >  1:151207/1‑94 (MQ=255)
                                                                             gTTCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATc             >  1:9629446/1‑94 (MQ=255)
                                                                               tCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCg           <  1:8467192/94‑1 (MQ=255)
                                                                               tCCATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCg           <  2:1375307/94‑1 (MQ=255)
                                                                                ccATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCgg          <  1:3493673/94‑1 (MQ=255)
                                                                                ccATCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCgg          >  2:6828128/1‑94 (MQ=255)
                                                                                  aTCTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGgtg        <  1:1078623/94‑1 (MQ=255)
                                                                                    cTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGGAGTCTGCATCGTTCTCTGGGGTATCCGGtgtg      >  1:4692617/1‑94 (MQ=255)
                                                                                    cTGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGtgtg      <  2:3565836/94‑1 (MQ=255)
                                                                                    cTGACAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGtgtg      <  1:2062562/94‑1 (MQ=255)
                                                                                     tGGCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGTGTgc     <  1:10247517/94‑1 (MQ=255)
                                                                                      ggCAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGTGTgcg    <  2:2859099/94‑1 (MQ=255)
                                                                                        cAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGTGTGCGcc  >  1:9290381/1‑94 (MQ=255)
                                                                                        cAGGCCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGTGTGCGcc  >  2:3939217/1‑94 (MQ=255)
                                                                                           |                                                                                          
TATAAGTTGCTCAGCAAGCTCATTTTCGCTGTTCTTCATCGTGCCGAAGGCTGCAGCCGTCAGCAGATGGCTGGTCAGTTCCATCTGGCAGACCATTTTGACCTGCGGGTAGAGCGCCGGGTTCCCGGCATGTGTCTGGCGGGGGAAGGCTGCATCGTTCTCTGGTGTATCCGGTGTGCGCC  >  NC_000913/4500409‑4500590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 242 total aligned reads displayed.
GCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAAACACAGA  >  NC_000913/4525743‑4525929
                                                                                             |                                                                                             
GCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGC                                                                                               >  SRR4302545.768047/1‑94 (MQ=60)
GCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGC                                                                                               >  SRR4302545.4186654/1‑94 (MQ=60)
GCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGC                                                                                               <  SRR4302545.396654/94‑1 (MQ=60)
GCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGC                                                                                               >  SRR4302545.4186653/1‑94 (MQ=60)
 CAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCT                                                                                              <  SRR4302545.2264782/94‑1 (MQ=60)
 CAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCT                                                                                              >  SRR4302545.10857100/1‑94 (MQ=60)
  AAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGGGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTG                                                                                             >  SRR4302545.7192196/1‑94 (MQ=60)
  AAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTG                                                                                             <  SRR4302545.4800499/94‑1 (MQ=60)
  AAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTG                                                                                             >  SRR4302545.2392853/1‑94 (MQ=60)
  AAAAACGCCGCCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTG                                                                                             <  SRR4302545.9819616/94‑1 (MQ=60)
   AAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGG                                                                                            >  SRR4302545.671220/1‑94 (MQ=60)
   AAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGG                                                                                            >  SRR4302545.8831953/1‑94 (MQ=60)
     AACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCC                                                                                          >  SRR4302545.9312050/1‑94 (MQ=60)
     AACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCC                                                                                          >  SRR4302545.9490461/1‑94 (MQ=60)
       CGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAA                                                                                        >  SRR4302545.4389806/1‑94 (MQ=60)
        GCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAAC                                                                                       <  SRR4302545.7335176/94‑1 (MQ=60)
        GCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAAC                                                                                       <  SRR4302545.753354/94‑1 (MQ=60)
         CCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACA                                                                                      >  SRR4302545.624216/1‑94 (MQ=60)
         CCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACA                                                                                      >  SRR4302545.6591263/1‑94 (MQ=60)
          CGTCCTTTTTAAAGGTGGTTGCGGTTACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGTCAACAC                                                                                     >  SRR4302545.5708303/1‑94 (MQ=60)
          CGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACAC                                                                                     >  SRR4302545.8983848/1‑94 (MQ=60)
          CGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACAC                                                                                     <  SRR4302545.2070829/94‑1 (MQ=60)
          CGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACAC                                                                                     >  SRR4302545.2542619/1‑94 (MQ=60)
          CGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACAC                                                                                     >  SRR4302545.10615471/1‑94 (MQ=60)
          CGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACAC                                                                                     >  SRR4302545.10615470/1‑94 (MQ=60)
          CGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACAC                                                                                     >  SRR4302545.1693435/1‑94 (MQ=60)
          CGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACAC                                                                                     <  SRR4302545.9035135/94‑1 (MQ=60)
          CGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACAC                                                                                     >  SRR4302545.9579109/1‑94 (MQ=60)
           GTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACG                                                                                    >  SRR4302545.3934403/1‑94 (MQ=60)
           GTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACG                                                                                    >  SRR4302545.6294726/1‑94 (MQ=60)
           GTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACG                                                                                    >  SRR4302545.10424282/1‑94 (MQ=60)
           GTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACG                                                                                    >  SRR4302545.6294725/1‑94 (MQ=60)
           GTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACG                                                                                    >  SRR4302545.5649840/1‑94 (MQ=60)
            TCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGA                                                                                   >  SRR4302545.1763791/1‑94 (MQ=60)
             CCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGAC                                                                                  >  SRR4302545.5009811/1‑94 (MQ=60)
             CCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGAC                                                                                  >  SRR4302545.902688/1‑94 (MQ=60)
             CCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGAC                                                                                  >  SRR4302545.6501486/1‑94 (MQ=60)
              CTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATAGTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACG                                                                                 <  SRR4302545.8851450/94‑1 (MQ=60)
              CTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACG                                                                                 <  SRR4302545.4648388/94‑1 (MQ=60)
              CTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACG                                                                                 >  SRR4302545.2537777/1‑94 (MQ=60)
               TTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCGCGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGG                                                                                <  SRR4302545.6763009/94‑1 (MQ=60)
               TTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGG                                                                                >  SRR4302545.4524375/1‑94 (MQ=60)
               TTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGG                                                                                >  SRR4302545.3798596/1‑94 (MQ=60)
               TTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGG                                                                                >  SRR4302545.9367777/1‑94 (MQ=60)
                TTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGT                                                                               <  SRR4302545.7923576/94‑1 (MQ=60)
                TTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTACTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGT                                                                               <  SRR4302545.10160691/94‑1 (MQ=60)
                 TTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTG                                                                              >  SRR4302545.7525257/1‑94 (MQ=60)
                   TAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTC                                                                            <  SRR4302545.5732557/94‑1 (MQ=60)
                   TAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTC                                                                            >  SRR4302545.2180774/1‑94 (MQ=60)
                   TAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTC                                                                            <  SRR4302545.4148427/94‑1 (MQ=60)
                    AAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCG                                                                           >  SRR4302545.8896122/1‑94 (MQ=60)
                    AAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCA                                                                           <  SRR4302545.6920476/94‑1 (MQ=60)
                    AAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCA                                                                           >  SRR4302545.4604614/1‑94 (MQ=60)
                    AAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCA                                                                           >  SRR4302545.6205852/1‑94 (MQ=60)
                    AAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCA                                                                           >  SRR4302545.9793762/1‑94 (MQ=60)
                    AAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGGGTCA                                                                           >  SRR4302545.500339/1‑94 (MQ=60)
                     AAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAG                                                                          <  SRR4302545.2761306/94‑1 (MQ=60)
                      AGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGG                                                                         >  SRR4302545.6720080/1‑94 (MQ=60)
                      AGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGG                                                                         <  SRR4302545.7720874/94‑1 (MQ=60)
                      AGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGG                                                                         >  SRR4302545.2490093/1‑94 (MQ=60)
                        GTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCA                                                                       >  SRR4302545.7293799/1‑94 (MQ=60)
                         TGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCAC                                                                      <  SRR4302545.4985259/94‑1 (MQ=60)
                          GGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCGCGTTTACCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACC                                                                     <  SRR4302545.7252824/94‑1 (MQ=60)
                          GGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACC                                                                     >  SRR4302545.7947223/1‑94 (MQ=60)
                          GGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACC                                                                     <  SRR4302545.9905958/94‑1 (MQ=60)
                          GGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACC                                                                     <  SRR4302545.9905957/94‑1 (MQ=60)
                          GGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACC                                                                     <  SRR4302545.10455592/94‑1 (MQ=60)
                          GGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACC                                                                     >  SRR4302545.3323300/1‑94 (MQ=60)
                          GGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACC                                                                     >  SRR4302545.10936400/1‑94 (MQ=60)
                           GTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCG                                                                    <  SRR4302545.10622466/94‑1 (MQ=60)
                           GTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGGGTCAGGCACCG                                                                    <  SRR4302545.10232088/94‑1 (MQ=60)
                            TTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGT                                                                   <  SRR4302545.718984/94‑1 (MQ=60)
                             TGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTT                                                                  <  SRR4302545.8025384/94‑1 (MQ=60)
                             TGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTT                                                                  >  SRR4302545.980547/1‑94 (MQ=60)
                              GCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTT                                                                 >  SRR4302545.3215757/1‑94 (MQ=60)
                              GCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTT                                                                 <  SRR4302545.7204785/94‑1 (MQ=60)
                              GCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTT                                                                 <  SRR4302545.964250/94‑1 (MQ=60)
                              GCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTT                                                                 <  SRR4302545.82080/94‑1 (MQ=60)
                               CGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTC                                                                >  SRR4302545.8289960/1‑94 (MQ=60)
                                GGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCT                                                               <  SRR4302545.9161011/94‑1 (MQ=60)
                                GGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCT                                                               >  SRR4302545.3393190/1‑94 (MQ=60)
                                GGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCT                                                               >  SRR4302545.10426243/1‑94 (MQ=60)
                                 GTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTT                                                              <  SRR4302545.4162515/94‑1 (MQ=60)
                                 GTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTT                                                              <  SRR4302545.7179181/94‑1 (MQ=60)
                                  TCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAGCACGACGGTGTCCGGGACCGTTTCTTT                                                             >  SRR4302545.8417563/1‑94 (MQ=60)
                                  TCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTT                                                             <  SRR4302545.2142243/94‑1 (MQ=60)
                                  TCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTT                                                             >  SRR4302545.8417562/1‑94 (MQ=60)
                                  TCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTT                                                             <  SRR4302545.4032183/94‑1 (MQ=60)
                                   CACACAGCCGTCGGCGATACTGAGCTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGTTGGCCAGCACGACGGTGTCAGGGACCGTTTCTTTG                                                            <  SRR4302545.9942481/94‑1 (MQ=60)
                                    ACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGA                                                           <  SRR4302545.598390/94‑1 (MQ=60)
                                    ACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGA                                                           <  SRR4302545.5454838/94‑1 (MQ=60)
                                     CACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGAC                                                          >  SRR4302545.9281201/1‑94 (MQ=60)
                                        AGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCG                                                       <  SRR4302545.9876349/94‑1 (MQ=60)
                                        AGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCG                                                       <  SRR4302545.4072201/94‑1 (MQ=60)
                                        AGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCG                                                       <  SRR4302545.6111573/94‑1 (MQ=60)
                                         GCCATCGGCGATACTGAGTTGTTCCTCCGCGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGT                                                      <  SRR4302545.650525/94‑1 (MQ=60)
                                         GCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGT                                                      >  SRR4302545.6205333/1‑94 (MQ=60)
                                         GCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGT                                                      <  SRR4302545.6060431/94‑1 (MQ=60)
                                         GCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGT                                                      <  SRR4302545.8123984/94‑1 (MQ=60)
                                         GCAATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGT                                                      <  SRR4302545.7493279/94‑1 (MQ=60)
                                          CCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTT                                                     <  SRR4302545.5390460/94‑1 (MQ=60)
                                          CCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTT                                                     <  SRR4302545.5294154/94‑1 (MQ=60)
                                          CCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTT                                                     >  SRR4302545.8724068/1‑94 (MQ=60)
                                            ctgGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCTTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTTACCCGTTTT                                                   <  SRR4302545.3884863/91‑1 (MQ=60)
                                            ATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTT                                                   >  SRR4302545.2165861/1‑94 (MQ=60)
                                             TCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTA                                                  <  SRR4302545.5376864/94‑1 (MQ=60)
                                             TCGGCGATACTGAGTTGTTCCTCAACGTTTTCCAGGCAGCCGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTA                                                  <  SRR4302545.10499298/94‑1 (MQ=60)
                                              CGGCGATTCTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAA                                                 <  SRR4302545.7846652/94‑1 (MQ=60)
                                               GGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAAC                                                <  SRR4302545.7700266/94‑1 (MQ=60)
                                               GGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAAC                                                <  SRR4302545.48637/94‑1 (MQ=60)
                                               GGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAAC                                                >  SRR4302545.7028986/1‑94 (MQ=60)
                                               GGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAAC                                                <  SRR4302545.6703988/94‑1 (MQ=60)
                                                GCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACA                                               <  SRR4302545.8339510/94‑1 (MQ=60)
                                                GCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACA                                               <  SRR4302545.192130/94‑1 (MQ=60)
                                                GCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACA                                               <  SRR4302545.4114793/94‑1 (MQ=60)
                                                 CGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAG                                              <  SRR4302545.4417553/94‑1 (MQ=60)
                                                   ATACTGAGTTGTTCCTCCGCGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCG                                            <  SRR4302545.3288571/94‑1 (MQ=60)
                                                    TACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGC                                           <  SRR4302545.8481063/94‑1 (MQ=60)
                                                    TACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGC                                           >  SRR4302545.3316310/1‑94 (MQ=60)
                                                    TACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGC                                           <  SRR4302545.8916459/94‑1 (MQ=60)
                                                     ACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCG                                          >  SRR4302545.9524098/1‑94 (MQ=60)
                                                     ACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCG                                          >  SRR4302545.10079608/1‑94 (MQ=60)
                                                     ACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCG                                          <  SRR4302545.4537819/94‑1 (MQ=60)
                                                      CTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGC                                         >  SRR4302545.3283054/1‑94 (MQ=60)
                                                       TGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCT                                        >  SRR4302545.6098141/1‑94 (MQ=60)
                                                       TGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCT                                        <  SRR4302545.2356443/94‑1 (MQ=60)
                                                       TGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCT                                        >  SRR4302545.604862/1‑94 (MQ=60)
                                                        GATTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTG                                       <  SRR4302545.8189956/94‑1 (MQ=60)
                                                        GAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTG                                       <  SRR4302545.1155167/94‑1 (MQ=60)
                                                        GAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTG                                       <  SRR4302545.7694668/94‑1 (MQ=60)
                                                        GAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTG                                       >  SRR4302545.4935448/1‑94 (MQ=60)
                                                         AGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGT                                      <  SRR4302545.10650467/94‑1 (MQ=60)
                                                         AGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGT                                      >  SRR4302545.426655/1‑94 (MQ=60)
                                                         AGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGT                                      <  SRR4302545.9077147/94‑1 (MQ=60)
                                                         AGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGT                                      <  SRR4302545.9418285/94‑1 (MQ=60)
                                                          GTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTC                                     >  SRR4302545.8589948/1‑94 (MQ=60)
                                                          GTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTC                                     >  SRR4302545.8589949/1‑94 (MQ=60)
                                                          GTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTC                                     <  SRR4302545.8928447/94‑1 (MQ=60)
                                                           TTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCG                                    <  SRR4302545.8018435/94‑1 (MQ=60)
                                                           TTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCG                                    <  SRR4302545.3799513/94‑1 (MQ=60)
                                                           TTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCG                                    <  SRR4302545.2155153/94‑1 (MQ=60)
                                                             ttcCCTCCTCGTTTTCCAGGCAGCCGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGT                                  <  SRR4302545.519560/91‑1 (MQ=60)
                                                              TTCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTA                                 >  SRR4302545.2827222/1‑94 (MQ=60)
                                                               TCCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTAC                                >  SRR4302545.1425925/1‑94 (MQ=60)
                                                                CCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACG                               >  SRR4302545.4651273/1‑94 (MQ=60)
                                                                CCTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACG                               <  SRR4302545.4782089/94‑1 (MQ=60)
                                                                 CTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGC                              >  SRR4302545.4326948/1‑94 (MQ=60)
                                                                 CTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGC                              >  SRR4302545.7159216/1‑94 (MQ=60)
                                                                 CTCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGC                              >  SRR4302545.7237250/1‑94 (MQ=60)
                                                                  TCCACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCG                             <  SRR4302545.7646777/94‑1 (MQ=60)
                                                                    CACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTC                           >  SRR4302545.5161467/1‑94 (MQ=60)
                                                                    CACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTC                           <  SRR4302545.5298826/94‑1 (MQ=60)
                                                                     ACGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCC                          >  SRR4302545.3044617/1‑94 (MQ=60)
                                                                      CGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCC                         <  SRR4302545.1765712/94‑1 (MQ=60)
                                                                      CGTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCC                         <  SRR4302545.4076212/94‑1 (MQ=60)
                                                                       GTTTTCCAGGGAGACGCCCGTGCTGGCCAACACGACGGTGTCAGTCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCG                        <  SRR4302545.4998133/94‑1 (MQ=60)
                                                                       GTTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCG                        <  SRR4302545.1981023/94‑1 (MQ=60)
                                                                        TTTTCCAGGCGGACGCCCGTGCGGGCAAACGCGACGGTGTCAGGCACCGTTTCTTTGTCCCGTTTTAGCAGCGCACTGTCGGGACGCGTCCCGG                       <  SRR4302545.6802950/94‑1 (MQ=53)
                                                                        TTTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGG                       <  SRR4302545.10118363/94‑1 (MQ=60)
                                                                         TTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGC                      <  SRR4302545.8941428/94‑1 (MQ=60)
                                                                         TTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGC                      <  SRR4302545.3320963/94‑1 (MQ=60)
                                                                         TTTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGC                      >  SRR4302545.2522228/1‑94 (MQ=60)
                                                                         TTTCCAGGCAGACGCCCGTCCTGGCCAACACGACGGTTGCAGCCACCGTTTCTTTGACCCGTTTTACCAGCGCGCTGTCGGTACGCGTCCCGGC                      <  SRR4302545.6224481/94‑1 (MQ=60)
                                                                          TTCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCG                     <  SRR4302545.1376374/94‑1 (MQ=60)
                                                                           TCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGG                    <  SRR4302545.10123408/94‑1 (MQ=60)
                                                                           TCCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGG                    >  SRR4302545.1792321/1‑94 (MQ=60)
                                                                            CCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGT                   <  SRR4302545.5043787/94‑1 (MQ=60)
                                                                            CCAGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTCTTAACAGCGCGCTGTCCGTACGCGTCCCGGCGGT                   >  SRR4302545.4081763/1‑94 (MQ=60)
                                                                            CCAGGCAGACCCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGT                   <  SRR4302545.8361260/94‑1 (MQ=60)
                                                                              TGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCA                 >  SRR4302545.6942173/1‑94 (MQ=60)
                                                                              AGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCA                 <  SRR4302545.8553480/94‑1 (MQ=60)
                                                                              AGGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCA                 <  SRR4302545.8050856/94‑1 (MQ=60)
                                                                               GTCAGACGCCCGTGCTGGCCAACACGACGGTGGCAGGCTCCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAG                <  SRR4302545.7067135/94‑1 (MQ=60)
                                                                               GGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAG                <  SRR4302545.1131247/94‑1 (MQ=60)
                                                                               GGCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAG                <  SRR4302545.6442884/94‑1 (MQ=60)
                                                                                GCAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGG               <  SRR4302545.10428295/94‑1 (MQ=60)
                                                                                 CAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGC              <  SRR4302545.2482966/94‑1 (MQ=60)
                                                                                 CAGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGC              >  SRR4302545.4814721/1‑94 (MQ=60)
                                                                                  AGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCC             >  SRR4302545.5825006/1‑94 (MQ=60)
                                                                                  AGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCC             <  SRR4302545.10550763/94‑1 (MQ=60)
                                                                                  AGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCC             >  SRR4302545.7368689/1‑94 (MQ=60)
                                                                                  AGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGGCAGGCC             >  SRR4302545.9606151/1‑94 (MQ=60)
                                                                                  AGACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGACAGGCC             >  SRR4302545.1320895/1‑94 (MQ=60)
                                                                                   GACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCA            >  SRR4302545.539140/1‑94 (MQ=60)
                                                                                   GACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCA            <  SRR4302545.6584573/94‑1 (MQ=60)
                                                                                    ACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAG           >  SRR4302545.6409762/1‑94 (MQ=60)
                                                                                    ACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAG           >  SRR4302545.2642349/1‑94 (MQ=60)
                                                                                    ACGCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAG           >  SRR4302545.6409761/1‑94 (MQ=60)
                                                                                      GCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAA         >  SRR4302545.4370989/1‑94 (MQ=60)
                                                                                      GCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAA         >  SRR4302545.9573094/1‑94 (MQ=60)
                                                                                      GCCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAA         <  SRR4302545.579289/94‑1 (MQ=60)
                                                                                       CCCGTGCTGGCCAACACGACGGTGTCATGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAAA        >  SRR4302545.3439086/1‑94 (MQ=60)
                                                                                       CCCGTGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGCCCAGAAA        >  SRR4302545.7059124/1‑94 (MQ=60)
                                                                                           TGCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAAACACA    <  SRR4302545.499253/94‑1 (MQ=60)
                                                                                            GCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAAACACAG   >  SRR4302545.3317087/1‑94 (MQ=60)
                                                                                            GCTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAAACACAG   <  SRR4302545.9278233/94‑1 (MQ=60)
                                                                                             CTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAAACACAGA  <  SRR4302545.10939594/94‑1 (MQ=60)
                                                                                             CTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAAACACAGA  <  SRR4302545.6210099/94‑1 (MQ=60)
                                                                                             CTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAAACACAGA  <  SRR4302545.4591619/94‑1 (MQ=60)
                                                                                             CTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAAACACAGA  >  SRR4302545.2964403/1‑94 (MQ=60)
                                                                                             |                                                                                             
GCAAAAACGCCGTCCTTTTTAAAGGTGGTTGCGGTCACACAGCCATCGGCGATACTGAGTTGTTCCTCCACGTTTTCCAGGCAGACGCCCGTGTTGGCCAACACGACGGTGTCAGGCACCGTTTCTTTGACCCGTTTTAACAGCGCGCTGTCGGTACGCGTCCCGGCGGTCAGGCCAGAAACACAGA  >  NC_000913/4525743‑4525929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑