Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 28,465 T→C V31A (GTG→GCG)  dapB → dihydrodipicolinate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091328,4650TC95.9% 699.3 / 11.5 220V31A (GTG→GCG) dapBdihydrodipicolinate reductase
Reads supporting (aligned to +/- strand):  ref base T (3/6);  new base C (105/106);  total (108/112)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

Only 100 of 231 total aligned reads displayed.

TGCATGATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGTGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACAGGCGTT  >  NC_000913/28375‑28553
                                                                                          |                                                                                        
tGCATGATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGgcgcgc                                                                                        >  1:4685550/1‑93 (MQ=255)
tGCATGATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGgcgcgc                                                                                        <  1:4397268/93‑1 (MQ=255)
tGCATGATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGgcgcgc                                                                                        >  2:1530221/1‑93 (MQ=255)
   atgatgCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGt                                                                                     >  2:5803772/1‑93 (MQ=255)
   atgatgCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGt                                                                                     <  1:1942021/93‑1 (MQ=255)
    tgatgCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGtt                                                                                    >  2:6113780/1‑93 (MQ=255)
    tgatgCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGtt                                                                                    >  1:2645321/1‑93 (MQ=255)
    tgatgCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGtt                                                                                    <  2:2275594/93‑1 (MQ=255)
    tgatgCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGtt                                                                                    >  2:238612/1‑93 (MQ=255)
    tgatgCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGtt                                                                                    <  1:759591/93‑1 (MQ=255)
     gatgCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTg                                                                                   <  1:255826/93‑1 (MQ=255)
     gatgCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTg                                                                                   <  1:4174295/93‑1 (MQ=255)
     gatgCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCAGGCATTAGAGGGCGCGCAGTTg                                                                                   >  1:3154610/1‑93 (MQ=255)
     gatgCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGACGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTg                                                                                   >  1:6067131/1‑93 (MQ=255)
      atnaaanCATCCGCGTTCCCATCGCGGGAGCCGGGCGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTgg                                                                                  <  1:1339041/89‑1 (MQ=255)
      atgCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTgg                                                                                  <  2:6065138/93‑1 (MQ=255)
      atgCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTgg                                                                                  >  1:4918293/1‑93 (MQ=255)
      atgCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTgg                                                                                  <  2:3646306/93‑1 (MQ=255)
       tgCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTggg                                                                                 <  1:643761/93‑1 (MQ=255)
       tgCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTggg                                                                                 >  2:2918949/1‑93 (MQ=255)
         cAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGgcg                                                                               >  1:2112326/1‑93 (MQ=255)
         cAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGGTGGgcg                                                                               >  1:1220374/1‑93 (MQ=255)
          aaaCATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGTGCAGTTGGgcgc                                                                              <  1:3982434/93‑1 (MQ=255)
          aaaCATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGgcgc                                                                              <  2:5436074/93‑1 (MQ=255)
          aaaCATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGgcgc                                                                              <  1:3583514/93‑1 (MQ=255)
           aaCATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGGTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGgcgct                                                                             >  2:249096/1‑93 (MQ=255)
            aCATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGgcgctg                                                                            <  1:5661444/93‑1 (MQ=255)
            aCATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGgcgctg                                                                            >  1:638705/1‑93 (MQ=255)
            aCATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGgcgctg                                                                            >  2:638810/1‑93 (MQ=255)
              aTCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGgcgctgcg                                                                          >  1:3655676/1‑93 (MQ=255)
              aTCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGgcgctgcg                                                                          >  1:2998364/1‑93 (MQ=255)
               tCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGgcgctgcgc                                                                         >  2:5026155/1‑93 (MQ=255)
                ccGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGgcgctgcgct                                                                        <  2:2675529/93‑1 (MQ=255)
                 cgcgTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGcgctgcgctg                                                                       >  2:4243520/1‑93 (MQ=255)
                  gcgTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTgg                                                                      <  1:4820150/93‑1 (MQ=255)
                  gcgTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTgg                                                                      <  2:1475065/93‑1 (MQ=255)
                   cgTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGa                                                                     >  1:4154542/1‑93 (MQ=255)
                   cgTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGa                                                                     >  1:547438/1‑93 (MQ=255)
                    gTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAg                                                                    <  1:2518725/93‑1 (MQ=255)
                    gTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAg                                                                    >  2:5264198/1‑93 (MQ=255)
                    gTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAg                                                                    >  2:369169/1‑93 (MQ=255)
                     ttGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGc                                                                   <  1:582539/93‑1 (MQ=255)
                     ttGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGc                                                                   >  2:4733031/1‑93 (MQ=255)
                     ttGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGc                                                                   >  1:2174987/1‑93 (MQ=255)
                     ttGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGCTGGGCGCTGCGCTGGAgg                                                                   >  2:2707263/1‑92 (MQ=255)
                     ttGCCATCGCGGGAGCCGGGGGGCGTATGGGACGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGAGCGCAGTTGGGCGCTGCGCTGGAGc                                                                   >  2:1867519/1‑93 (MQ=255)
                      tGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCg                                                                  <  1:3282353/93‑1 (MQ=255)
                          aTCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGag                                                              >  2:1647042/1‑92 (MQ=255)
                          aTCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGaa                                                              <  1:2065835/93‑1 (MQ=255)
                          aTCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGaa                                                              <  2:5093217/93‑1 (MQ=255)
                              cgGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGAt                                                          >  1:851230/1‑93 (MQ=255)
                              cgGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGAt                                                          >  1:2801569/1‑93 (MQ=255)
                               gggAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGAtc                                                         >  2:4295747/1‑93 (MQ=255)
                                  aGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGAtcttc                                                      <  2:6195952/93‑1 (MQ=255)
                                   gCCGGGGTGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGAtcttct                                                     <  2:3868312/93‑1 (MQ=255)
                                   gCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGAtcttct                                                     >  1:1724447/1‑93 (MQ=255)
                                     cGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCttt                                                   >  2:3555402/1‑93 (MQ=255)
                                       gggggCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTAc                                                 <  2:3346664/93‑1 (MQ=255)
                                       gggggCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTAc                                                 >  2:5700611/1‑93 (MQ=255)
                                        ggggCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACt                                                >  1:3048641/1‑93 (MQ=255)
                                        ggggCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACt                                                <  1:1661012/93‑1 (MQ=255)
                                         gggCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTg                                               >  2:4789165/1‑93 (MQ=255)
                                         gggCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTg                                               <  2:5513445/93‑1 (MQ=255)
                                           gCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTggg                                             >  2:3473073/1‑93 (MQ=255)
                                              tATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAg                                          <  1:1649863/93‑1 (MQ=255)
                                              tATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAg                                          >  2:6044942/1‑93 (MQ=255)
                                              tATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAg                                          >  1:1514222/1‑93 (MQ=255)
                                               aTGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGc                                         >  1:1310879/1‑93 (MQ=255)
                                                tGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCg                                        >  1:5934856/1‑93 (MQ=255)
                                                tGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAACGTGAAGGATCTTCTTTACTGGGCAGCg                                        >  1:6215731/1‑93 (MQ=255)
                                                 gggCCGCCAGTTGATTCAGGCTGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGa                                       >  1:5229930/1‑93 (MQ=255)
                                                  ggCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGAc                                      <  1:1588708/93‑1 (MQ=255)
                                                    ccgccAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGc                                    <  1:4335585/93‑1 (MQ=255)
                                                     cgccAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGcc                                   <  2:1379575/93‑1 (MQ=255)
                                                      gccAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCg                                  >  1:914184/1‑93 (MQ=255)
                                                      gccAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCg                                  <  1:3956757/93‑1 (MQ=255)
                                                         aGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTg                               >  2:4058018/1‑93 (MQ=255)
                                                           ttGATTCAGGCGGCGCTGTCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAg                             >  2:2549222/1‑93 (MQ=255)
                                                            tGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGc                            <  1:1020605/93‑1 (MQ=255)
                                                            tGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGc                            <  1:1698332/93‑1 (MQ=255)
                                                             gATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCt                           <  1:612912/93‑1 (MQ=255)
                                                                tCAGGCGGCGCTGGCATTAGAGGGCGTGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGc                        >  1:1026069/1‑93 (MQ=255)
                                                                tCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGc                        <  2:4436319/93‑1 (MQ=255)
                                                                tCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGc                        >  1:2420536/1‑93 (MQ=255)
                                                                tCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGc                        <  1:410691/93‑1 (MQ=255)
                                                                  aGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCg                      <  1:3834893/93‑1 (MQ=255)
                                                                  aGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCg                      <  1:4442240/93‑1 (MQ=255)
                                                                      ggcgCTGGCATTAGAGGGCGCGCAGTTGGGCTCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGc                  <  2:579523/93‑1 (MQ=255)
                                                                      ggcgCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGc                  <  2:68197/93‑1 (MQ=255)
                                                                          cTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCggg              >  1:1094029/1‑93 (MQ=255)
                                                                           tGGCATTAGAGGGCGCGCAGTTTGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGa             <  2:2163442/93‑1 (MQ=255)
                                                                            ggCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGaa            <  1:3253406/93‑1 (MQ=255)
                                                                             gCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGaaa           >  2:1313788/1‑93 (MQ=255)
                                                                                 tAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACAg       >  1:486419/1‑93 (MQ=255)
                                                                                 tAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACAg       <  2:4937374/93‑1 (MQ=255)
                                                                                  agagGGCGTGCAGTTGGGCGCTGCGCTGGAGCGTGAAGTATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGCCCGGAGCCGGGAAAACAgg      <  1:3479794/93‑1 (MQ=255)
                                                                                  agagGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACAgg      <  1:5448563/93‑1 (MQ=255)
                                                                                  agagGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACAgg      >  2:5040906/1‑93 (MQ=255)
                                                                                  agagGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACAgg      >  2:1726476/1‑93 (MQ=255)
                                                                                    agGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACAGGCg    >  1:2474950/1‑93 (MQ=255)
                                                                                          |                                                                                        
TGCATGATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGTGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACAGGCGTT  >  NC_000913/28375‑28553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTATGCATGATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGTGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACAGGCGTTACC  >  NC_000913/28372‑28556
                                                                                             |                                                                                           
CTATGCATGATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGC                                                                                             >  SRR4302537.961680/1‑94 (MQ=60)
 TATGCATGATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCG                                                                                            >  SRR4302537.2293448/1‑94 (MQ=60)
  ATGCATGATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGC                                                                                           >  SRR4302537.4717960/1‑94 (MQ=60)
   TGCATGATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCA                                                                                          <  SRR4302537.4427607/94‑1 (MQ=60)
     CATGATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGT                                                                                        <  SRR4302537.1712802/94‑1 (MQ=60)
      ATGATGCAAACATCCGCGTTGTCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGAGGCGCTGGCATTAGAGTGCGCGCAGTG                                                                                       >  SRR4302537.3921572/1‑94 (MQ=60)
      ATGATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTT                                                                                       >  SRR4302537.3292724/1‑94 (MQ=60)
      ATGATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTT                                                                                       <  SRR4302537.1955522/94‑1 (MQ=60)
      ATGATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTT                                                                                       >  SRR4302537.2663823/1‑94 (MQ=60)
       TGATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTG                                                                                      <  SRR4302537.764783/94‑1 (MQ=60)
       TGATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTG                                                                                      >  SRR4302537.1336890/1‑94 (MQ=60)
       TGATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCAGGCATTAGAGGGCGCGCAGTTG                                                                                      >  SRR4302537.3176758/1‑94 (MQ=60)
       TGATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGACGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTG                                                                                      >  SRR4302537.6108813/1‑94 (MQ=60)
        GATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGG                                                                                     <  SRR4302537.257644/94‑1 (MQ=60)
        GATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGG                                                                                     >  SRR4302537.4952224/1‑94 (MQ=60)
        GATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGG                                                                                     <  SRR4302537.4203441/94‑1 (MQ=60)
        GATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGG                                                                                     <  SRR4302537.1604290/94‑1 (MQ=60)
         ATNAAANCATCCGCGTTCCCATCGCGGGAGCCGGGCGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGG                                                                                    <  SRR4302537.1348366/94‑1 (MQ=60)
         ATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGG                                                                                    <  SRR4302537.4452260/94‑1 (MQ=60)
          TGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGC                                                                                   <  SRR4302537.648148/94‑1 (MQ=60)
          TGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGC                                                                                   <  SRR4302537.3182938/94‑1 (MQ=60)
           GCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCG                                                                                  >  SRR4302537.2127210/1‑94 (MQ=60)
           GCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCG                                                                                  <  SRR4302537.1534323/94‑1 (MQ=60)
           GCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGGTGGGCG                                                                                  >  SRR4302537.1228862/1‑94 (MQ=60)
             AAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGTGCAGTTGGGCGCT                                                                                <  SRR4302537.4010313/94‑1 (MQ=60)
             AAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCT                                                                                <  SRR4302537.3608773/94‑1 (MQ=60)
              AACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTG                                                                               >  SRR4302537.643054/1‑94 (MQ=60)
               ACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGC                                                                              <  SRR4302537.5700187/94‑1 (MQ=60)
               ACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGC                                                                              >  SRR4302537.3646590/1‑94 (MQ=60)
                CATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCG                                                                             >  SRR4302537.3019266/1‑94 (MQ=60)
                CATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCG                                                                             >  SRR4302537.3681402/1‑94 (MQ=60)
                CATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCG                                                                             <  SRR4302537.4899894/94‑1 (MQ=60)
                CATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCG                                                                             >  SRR4302537.2503052/1‑94 (MQ=60)
                  TCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGTGCAGTTGGGCGCTGCGCT                                                                           <  SRR4302537.2662121/94‑1 (MQ=60)
                    CGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGG                                                                         >  SRR4302537.4075311/1‑94 (MQ=60)
                    CGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGG                                                                         >  SRR4302537.535931/1‑94 (MQ=60)
                     GCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGA                                                                        >  SRR4302537.551211/1‑94 (MQ=60)
                     GCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGA                                                                        >  SRR4302537.4183553/1‑94 (MQ=60)
                     GCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGA                                                                        <  SRR4302537.4853432/94‑1 (MQ=60)
                     GCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGACGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGA                                                                        >  SRR4302537.551210/1‑94 (MQ=60)
                      CGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGTG                                                                       >  SRR4302537.3154954/1‑94 (MQ=60)
                       GTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGC                                                                      <  SRR4302537.2536415/94‑1 (MQ=60)
                       GTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGC                                                                      >  SRR4302537.2190317/1‑94 (MQ=60)
                       GTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGC                                                                      >  SRR4302537.2067733/1‑94 (MQ=60)
                       GTTGCCATCGCGGGAGCCGGCGGGCGTATGGGCCGCCAGTTGATTCAGACGGGGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGC                                                                      >  SRR4302537.3829352/1‑94 (MQ=60)
                        TTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCG                                                                     <  SRR4302537.586526/94‑1 (MQ=60)
                         TGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGT                                                                    <  SRR4302537.918505/94‑1 (MQ=60)
                         TGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGT                                                                    <  SRR4302537.3305524/94‑1 (MQ=60)
                         TGCCATCGCGGGAGCCGGGGGGCGTATGGGACGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGG                                                                    >  SRR4302537.1553538/1‑94 (MQ=60)
                          GCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTG                                                                   >  SRR4302537.4239484/1‑94 (MQ=60)
                          GCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTG                                                                   <  SRR4302537.1028816/94‑1 (MQ=60)
                            CATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAA                                                                 >  SRR4302537.3703239/1‑94 (MQ=60)
                             CTCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAG                                                                <  SRR4302537.1666787/94‑1 (MQ=60)
                             ATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAG                                                                <  SRR4302537.2080344/94‑1 (MQ=60)
                             ATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAG                                                                >  SRR4302537.1269516/1‑94 (MQ=60)
                             ATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAG                                                                >  SRR4302537.3285273/1‑94 (MQ=60)
                               CGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGA                                                              <  SRR4302537.6434343/94‑1 (MQ=60)
                                GCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGAT                                                             >  SRR4302537.2821154/1‑94 (MQ=60)
                                GCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGAT                                                             >  SRR4302537.857065/1‑94 (MQ=60)
                                 CGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATC                                                            <  SRR4302537.211012/94‑1 (MQ=60)
                                  GGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCT                                                           <  SRR4302537.3324923/94‑1 (MQ=60)
                                  GGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCT                                                           <  SRR4302537.4297802/94‑1 (MQ=60)
                                     AGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCT                                                        <  SRR4302537.821539/94‑1 (MQ=60)
                                     AGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCT                                                        >  SRR4302537.1736496/1‑94 (MQ=60)
                                       CCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTT                                                      <  SRR4302537.5995093/94‑1 (MQ=60)
                                       CCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGACATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTT                                                      <  SRR4302537.2743785/94‑1 (MQ=60)
                                        CGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGGGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTA                                                     <  SRR4302537.4207837/94‑1 (MQ=60)
                                          GGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACT                                                   >  SRR4302537.3069883/1‑94 (MQ=60)
                                          GGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACT                                                   >  SRR4302537.6233003/1‑94 (MQ=60)
                                          GGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACT                                                   >  SRR4302537.2565147/1‑94 (MQ=60)
                                           GGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTG                                                  <  SRR4302537.1672653/94‑1 (MQ=60)
                                               CGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCA                                              >  SRR4302537.1365166/1‑94 (MQ=60)
                                                GTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAG                                             <  SRR4302537.1250514/94‑1 (MQ=60)
                                                GTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAG                                             <  SRR4302537.190975/94‑1 (MQ=60)
                                                GTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAG                                             >  SRR4302537.1524802/1‑94 (MQ=60)
                                                GTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGAGTGAAGGATCTTCTTTACTGGGCAG                                             >  SRR4302537.5638975/1‑94 (MQ=60)
                                                 TATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGC                                            <  SRR4302537.1661439/94‑1 (MQ=60)
                                                 TATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGC                                            >  SRR4302537.1320003/1‑94 (MQ=60)
                                                 TATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGC                                            >  SRR4302537.4418927/1‑94 (MQ=60)
                                                  ATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCG                                           >  SRR4302537.5975585/1‑94 (MQ=60)
                                                  ATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAACGTGAAGGATCTTCTTTACTGGGCAGCG                                           >  SRR4302537.6258530/1‑94 (MQ=60)
                                                   TGGGCCGCCAGTTGATTCAGGCTGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGA                                          >  SRR4302537.5265896/1‑94 (MQ=60)
                                                   TGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGA                                          >  SRR4302537.3046919/1‑94 (MQ=60)
                                                   TGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGA                                          >  SRR4302537.1677165/1‑94 (MQ=60)
                                                     GGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACG                                        <  SRR4302537.1707992/94‑1 (MQ=60)
                                                     GGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACG                                        <  SRR4302537.1599813/94‑1 (MQ=60)
                                                       CCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCC                                      <  SRR4302537.4365690/94‑1 (MQ=60)
                                                       CCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCC                                      <  SRR4302537.4931439/94‑1 (MQ=60)
                                                        CGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCG                                     >  SRR4302537.920495/1‑94 (MQ=60)
                                                         GCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGG                                    <  SRR4302537.3984614/94‑1 (MQ=60)
                                                         GCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGG                                    <  SRR4302537.3984443/94‑1 (MQ=60)
                                                           CAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTG                                  >  SRR4302537.458353/1‑94 (MQ=60)
                                                            AGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGA                                 >  SRR4302537.6011440/1‑94 (MQ=60)
                                                              TTGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGC                               >  SRR4302537.1166190/1‑94 (MQ=60)
                                                               TGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCT                              <  SRR4302537.1710207/94‑1 (MQ=60)
                                                               TGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCT                              <  SRR4302537.1027638/94‑1 (MQ=60)
                                                               TGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCT                              <  SRR4302537.2441821/94‑1 (MQ=60)
                                                               TGATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCT                              <  SRR4302537.822461/94‑1 (MQ=60)
                                                                TATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTG                             <  SRR4302537.382942/94‑1 (MQ=60)
                                                                GATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTG                             <  SRR4302537.617093/94‑1 (MQ=60)
                                                                 ATTCAGGCGGCGCTGGCATTAGAGGGCGTGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGG                            <  SRR4302537.6314331/94‑1 (MQ=60)
                                                                 ATTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGG                            >  SRR4302537.5914789/1‑94 (MQ=60)
                                                                  TTCAGGCGGCGCTGGCATTAGAGGGCGTGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGC                           >  SRR4302537.1033134/1‑94 (MQ=60)
                                                                  TTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGC                           >  SRR4302537.6124015/1‑94 (MQ=60)
                                                                  TTCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGC                           >  SRR4302537.2437593/1‑94 (MQ=60)
                                                                   TCAGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCC                          <  SRR4302537.413534/94‑1 (MQ=60)
                                                                     AGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGG                        <  SRR4302537.3861769/94‑1 (MQ=60)
                                                                     AGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGG                        <  SRR4302537.4472920/94‑1 (MQ=60)
                                                                     AGGCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGG                        <  SRR4302537.4861581/94‑1 (MQ=60)
                                                                       GCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAG                      <  SRR4302537.3066617/94‑1 (MQ=60)
                                                                       GCGGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAG                      >  SRR4302537.4778182/1‑94 (MQ=60)
                                                                         GGCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCC                    <  SRR4302537.4145679/94‑1 (MQ=60)
                                                                         GGCGCTGGCATTAGAGGGCGCGCAGGTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCC                    >  SRR4302537.2473882/1‑94 (MQ=60)
                                                                          GCGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCG                   <  SRR4302537.2853095/94‑1 (MQ=60)
                                                                           CGCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGG                  <  SRR4302537.5794505/94‑1 (MQ=60)
                                                                            GCTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGG                 >  SRR4302537.1101585/1‑94 (MQ=60)
                                                                             CTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGA                >  SRR4302537.2276857/1‑94 (MQ=60)
                                                                             CTGGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGA                <  SRR4302537.4194103/94‑1 (MQ=60)
                                                                               GGCATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAA              <  SRR4302537.3276351/94‑1 (MQ=60)
                                                                                 CATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAAC            >  SRR4302537.5624616/1‑94 (MQ=60)
                                                                                  ATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACA           <  SRR4302537.2634130/94‑1 (MQ=60)
                                                                                  ATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACA           >  SRR4302537.724386/1‑94 (MQ=60)
                                                                                  ATTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACA           >  SRR4302537.1095728/1‑94 (MQ=60)
                                                                                   TTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACAG          >  SRR4302537.489759/1‑94 (MQ=60)
                                                                                   TTAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACAG          <  SRR4302537.200303/94‑1 (MQ=60)
                                                                                    TAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGTAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACAGG         <  SRR4302537.2141554/94‑1 (MQ=60)
                                                                                    TAGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACAGG         <  SRR4302537.6429860/94‑1 (MQ=60)
                                                                                     AGAGGGCGTGCAGTTGGGCGCTGCGCTGGAGCGTGAAGTATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGCCCGGAGCCGGGAAAACAGGC        <  SRR4302537.3504414/94‑1 (MQ=60)
                                                                                     AGAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACAGGC        <  SRR4302537.5485728/94‑1 (MQ=60)
                                                                                      GAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACAGGCG       >  SRR4302537.2492349/1‑94 (MQ=60)
                                                                                      GAGGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACAGGCG       <  SRR4302537.4617277/94‑1 (MQ=60)
                                                                                        GGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACAGGCGTT     >  SRR4302537.3607073/1‑94 (MQ=60)
                                                                                        GGGCGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACAGGCGTT     >  SRR4302537.5032488/1‑94 (MQ=60)
                                                                                           CGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACAGGCGTTACC  <  SRR4302537.3580123/94‑1 (MQ=60)
                                                                                           CGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACAGGCGTTACC  >  SRR4302537.6022437/1‑94 (MQ=60)
                                                                                           CGCGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACAGGCGTTACC  <  SRR4302537.3147/94‑1 (MQ=60)
                                                                                             |                                                                                           
CTATGCATGATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAGTTGATTCAGGCGGCGCTGGCATTAGAGGGCGTGCAGTTGGGCGCTGCGCTGGAGCGTGAAGGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACAGGCGTTACC  >  NC_000913/28372‑28556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑