Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 63,753 A→G G676G (GGT→GGC)  polB ← DNA polymerase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091363,7530AG92.3% 262.2 / 5.7 91G676G (GGT→GGC) polBDNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (3/4);  new base G (75/9);  total (78/13)
Fisher's exact test for biased strand distribution p-value = 7.21e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 130 total aligned reads displayed.

AGCGGCGCGTACATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATAGCTCCTGCTGAAACTG  >  NC_000913/63663‑63842
                                                                                          |                                                                                         
tgcggccGGTACATGAGGCGCCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGcc                                                                                         <  1:3132126/92‑1 (MQ=255)
aGCGGCGCGTACATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGcc                                                                                         >  2:2497498/1‑93 (MQ=255)
aGCGGCGCGTACATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGcc                                                                                         <  1:3129223/93‑1 (MQ=255)
aGCGGCGCGTACATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGcc                                                                                         <  1:3132125/93‑1 (MQ=255)
 gcggcgCGTACATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGccc                                                                                        <  2:1671283/93‑1 (MQ=255)
 gcggcgCGTACATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGccc                                                                                        <  1:5723578/93‑1 (MQ=255)
   ggcgCGTACATGAGGCGACACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGc                                                                                      >  1:775506/1‑93 (MQ=255)
    gcgcGTACAGGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTcgcccgcc                                                                                     <  2:5468603/93‑1 (MQ=255)
       cgTACATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGAGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATc                                                                                  >  1:6074701/1‑93 (MQ=255)
        gTACATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCa                                                                                 >  2:1710711/1‑93 (MQ=255)
         tACATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAg                                                                                >  2:87588/1‑93 (MQ=255)
          aCATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGt                                                                               >  2:6383503/1‑93 (MQ=255)
           cATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGtt                                                                              >  2:6251073/1‑93 (MQ=255)
           cATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGtt                                                                              >  2:6010226/1‑93 (MQ=255)
           cATGAGGCGGCACATTACGCTGACACTCGCCCAGGGGGGGGCAACGGCGTTTACGAAAAACCAGTCCCCCATCCGGTCCGCCCGCCATCAGtt                                                                              <  2:6416195/93‑1 (MQ=255)
            aTGAGGCGGCACATTACGCTGATCCTCGCTCAGCGGACGGCGAAGGCTTTTACGGTAACCCAGTCGCGCATCCAGTTCGCCCGCCATCAGttt                                                                             <  2:939116/93‑1 (MQ=255)
             tGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTg                                                                            >  1:489928/1‑93 (MQ=255)
             tGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTCTg                                                                            >  2:3509953/1‑93 (MQ=255)
             tGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCATCAGTTTg                                                                            >  2:5662748/1‑93 (MQ=255)
             tGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACAAGTCGCGGATCCAGTTCGCCCGCCATCAGTTTg                                                                            >  2:1391616/1‑93 (MQ=255)
              gAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGt                                                                           >  1:3741059/1‑93 (MQ=255)
               aGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTc                                                                          >  1:2791245/1‑93 (MQ=255)
                 gcggcACATTAGGCTGATCCTCGCCCAGCGGAGGGCGAAGGTGTTTACGAAAAGCCGCTCGCGCTTCCATTTCCCCCGCCATCAGTTTGTCGa                                                                        <  2:934890/93‑1 (MQ=255)
                 gcggcACATTACGCTGATCCCGGCCAAGGGGACGGCGAAGGCGTTTACGGAAACCCAGCCGCGCATCCATTCCGCCCGCCATCAGTTTGTCGa                                                                        <  2:4790005/93‑1 (MQ=255)
                  cggcACATTCCGCTGATCCTCGCTCAGCGGACGGCGAGGGCTTTTACGGAAACCCAGCCGCGCACCCAGTTCGCCCGCCATCAGTTTGTCGAt                                                                       <  2:3652598/93‑1 (MQ=255)
                   ggcACATTCCGCTGATCCTCGCTCAGCGGACGGCGAGGGCTTTTACGGTAACCCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATg                                                                      <  2:2265012/93‑1 (MQ=255)
                   ggcACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATg                                                                      >  1:2154167/1‑93 (MQ=255)
                   ggcACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGATCGCCCGCCATCAGTTTGTCGATg                                                                      >  2:3987866/1‑93 (MQ=255)
                    gcACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATgg                                                                     >  2:2483187/1‑93 (MQ=255)
                      acaTTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGtt                                                                   >  1:807843/1‑93 (MQ=255)
                        tttccGCTAATCCTCGCCCGGCGGACGGCGAGGTCTTTTAGGGAAACCCAGTCGCGCATCCATTTCGCCCGCCATCAGTTTGTCGATGGTTTc                                                                 <  2:2285147/89‑1 (MQ=255)
                           aCGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTcgct                                                              >  1:1356365/1‑92 (MQ=255)
                            ctcTGATGCCCGCCGAGCGGACGGGAAAGCTGTTTAGGGAAACCCATTCCCGCCCCCCGTCCGCCCGCCATCAGTTTGTCGATGGTTTCGCGt                                                             <  2:6198845/91‑1 (MQ=255)
                             gCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTa                                                            >  2:2311892/1‑93 (MQ=255)
                             gCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTa                                                            >  1:4531465/1‑93 (MQ=255)
                             gCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCATCAGTTTGTCGATGGTTTCGCGTa                                                            <  1:494936/93‑1 (MQ=255)
                              cTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTAc                                                           >  2:3696214/1‑93 (MQ=255)
                              cTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTAc                                                           >  1:5324198/1‑93 (MQ=255)
                               tGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTata                                                          >  2:6335712/1‑91 (MQ=255)
                                gATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACat                                                         >  1:3900779/1‑93 (MQ=255)
                                   ccTCGCGCAGCGGGGGGGGAAGGTGTTTACGGAAAACCGGTCGCGCACCCAGTCCCCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATAtt                                                      <  2:4987771/92‑1 (MQ=255)
                                   aCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATAtt                                                      >  2:245451/1‑93 (MQ=255)
                                   aCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATAtt                                                      >  2:3767326/1‑93 (MQ=255)
                                   aCTCGCGCCGGGGAGGGCGAAGGTGTTTACGGTAACCCGGTCGCCCACCCAGTCCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATAtt                                                      <  2:1728139/93‑1 (MQ=255)
                                    cccGCTCAGCGGGGGGCGAGGGCTTTTCCGGTAACCCGGTCCCCCACCCAGTCCCCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTc                                                     <  1:1202226/91‑1 (MQ=255)
                                    cTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTc                                                     >  1:5806215/1‑93 (MQ=255)
                                    cTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGGTTCGCGTACATATTc                                                     >  2:529763/1‑93 (MQ=255)
                                    cTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTc                                                     <  2:4687040/93‑1 (MQ=255)
                                     tCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTcc                                                    >  2:2298682/1‑93 (MQ=255)
                                      cGCTCGGGGGACGGCGAGGGCTTTTCGGGTAACCCGCTCGCCCATCTGTTCCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCt                                                   <  2:1115405/93‑1 (MQ=255)
                                        cTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGa                                                 >  2:4604901/1‑93 (MQ=255)
                                        cTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGa                                                 >  2:1838254/1‑93 (MQ=255)
                                         tCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGat                                                >  2:601867/1‑93 (MQ=255)
                                          gaGCGGGCGGAGAAGGCTTTGACGAAACCCCAGTCGCGCATCCAGTTCCCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGata                                               <  1:642873/92‑1 (MQ=255)
                                          cggCGGAGGGCGAGGGATTTTACGTAAACCCAGCCGGCTATCCTGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGata                                               <  2:503983/91‑1 (MQ=255)
                                           aGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGatat                                              >  1:4600207/1‑93 (MQ=255)
                                           aGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGatat                                              >  2:4639161/1‑93 (MQ=255)
                                           aGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGatat                                              >  2:309785/1‑93 (MQ=255)
                                            gCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATg                                             >  1:4400081/1‑93 (MQ=255)
                                            gCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATg                                             >  1:373990/1‑93 (MQ=255)
                                              ggggggCGAAGGGTTTTAGGGTAACCCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGc                                           <  2:4110238/89‑1 (MQ=255)
                                              ggACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGc                                           >  2:2329615/1‑93 (MQ=255)
                                               gACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCt                                          >  2:6133077/1‑93 (MQ=255)
                                                 gggCGAAGGGTTTTACGGAAACCCTGGCGCACACCCAGTCCCCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCg                                        <  1:1959684/92‑1 (MQ=255)
                                                 cGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTcc                                        >  2:1138552/1‑92 (MQ=255)
                                                   gCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGtt                                      >  1:921121/1‑93 (MQ=255)
                                                   gCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGtt                                      >  1:4131365/1‑93 (MQ=255)
                                                     gAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGc                                    >  1:1292028/1‑93 (MQ=255)
                                                       aGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCCTTGCgg                                  >  1:5764188/1‑93 (MQ=255)
                                                        ggCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGGTGCCGa                                 >  1:1445713/1‑93 (MQ=255)
                                                        ggCGTTTACGGTAAACCAGTCGCCCATCCAGTTCACCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGa                                 <  1:4057245/93‑1 (MQ=255)
                                                         gtttttACGATAACCCACTCGCGCATCCAGTCCCCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGaa                                <  2:6461008/90‑1 (MQ=255)
                                                         gCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGaa                                <  2:1029915/93‑1 (MQ=255)
                                                         gCGTTGACGATAACCCAGGCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGaa                                <  2:2869311/93‑1 (MQ=255)
                                                          cGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAg                               >  2:3756282/1‑93 (MQ=255)
                                                           gTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGCTGCGGgaga                              >  1:1221997/1‑93 (MQ=255)
                                                            tttACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGGAGAt                             >  1:5106603/1‑93 (MQ=255)
                                                             ttAGGGAAACCCAGTCGCGCACCCGTTTCCCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATg                            <  1:2210003/93‑1 (MQ=255)
                                                             ttACGGTAACCCACTCGCGCACCCAGTCCCCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATg                            <  2:1399454/93‑1 (MQ=255)
                                                             ttACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGGAGATg                            >  2:231019/1‑93 (MQ=255)
                                                             ttACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGGTGCGGGAGATg                            >  1:1518379/1‑93 (MQ=255)
                                                             ttACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGCTGCGGAAGGTg                            >  2:476528/1‑93 (MQ=255)
                                                              tACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGCTGTGGAAGATgg                           >  2:4287303/1‑92 (MQ=255)
                                                                 ggTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGGAGATGCGCa                        >  2:3119359/1‑93 (MQ=255)
                                                                  gaaaaCCAGTCGCCCATCCAGTCCCCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAg                       <  1:3529078/91‑1 (MQ=255)
                                                                  gTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGGAGATGTGCAg                       >  1:1843588/1‑93 (MQ=255)
                                                                      aCCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGgat                   >  1:3315454/1‑93 (MQ=255)
                                                                          gTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGGAGAAGTGCAGGTATAgat               >  1:6240147/1‑91 (MQ=255)
                                                                          gTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCGGGGATAGCt               >  2:1572836/1‑93 (MQ=255)
                                                                          gTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGCTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGAGCAGGTATAGCt               >  1:3372309/1‑93 (MQ=255)
                                                                            cgcaCATCGAGTCCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATAGCTcc             <  1:89949/93‑1 (MQ=255)
                                                                             ccgcACCCAGTCCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATAGCTCct            <  2:357755/92‑1 (MQ=255)
                                                                               ccATCCTGTTCCCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATAGCTCctgc          <  1:1846770/92‑1 (MQ=255)
                                                                                 aTCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATAGCTCctgctg        >  2:3118170/1‑93 (MQ=255)
                                                                                 aTCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGGATAGGTCccgctg        >  1:358253/1‑93 (MQ=255)
                                                                                 aTCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGCTGCGGAAGATGCGCAGGGATATCTTccgctg        >  2:6262314/1‑93 (MQ=255)
                                                                                    cAGTCCCCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATAGCTCCTGCTGaaa     <  1:2604374/93‑1 (MQ=255)
                                                                                     aGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGGAAATGGGCCGGTATAAGCCCTTCTGaaaa    >  2:3463993/1‑92 (MQ=255)
                                                                                      cttCCCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATAGCTCCTGCTGAAAct   <  1:1653657/92‑1 (MQ=255)
                                                                                       ttCACCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGGATAGCTTCTGCTGAAActg  >  1:3630153/1‑93 (MQ=255)
                                                                                          |                                                                                         
AGCGGCGCGTACATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATAGCTCCTGCTGAAACTG  >  NC_000913/63663‑63842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATAGCTCCTGCTGAAACTGCTGG  >  NC_000913/63660‑63846
                                                                                             |                                                                                             
GCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCG                                                                                               <  SRR4302537.5536989/94‑1 (MQ=60)
 CGAGCGGCGCGTACATGAGGCGGCACACTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGC                                                                                              >  SRR4302537.2720586/1‑94 (MQ=60)
  GAGCGGCGCGTACATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCC                                                                                             >  SRR4302537.4299509/1‑94 (MQ=60)
   tgcggccgGTACATGAGGCGCCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCC                                                                                            <  SRR4302537.3154101/86‑1 (MQ=60)
   AGCGGCGCGTACATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCC                                                                                            <  SRR4302537.3151159/94‑1 (MQ=60)
   AGCGGCGCGTACATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCC                                                                                            <  SRR4302537.3154100/94‑1 (MQ=60)
    GCGGCGCGTACATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCG                                                                                           <  SRR4302537.2091499/94‑1 (MQ=60)
    GCGGCGCGTACATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCG                                                                                           <  SRR4302537.5762760/94‑1 (MQ=60)
     CGGCGCGTACATGAGGCGACACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGC                                                                                          >  SRR4302537.780822/1‑94 (MQ=60)
         GCGTACATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGAGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATC                                                                                      >  SRR4302537.6116447/1‑94 (MQ=60)
             ACATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTT                                                                                  >  SRR4302537.1839322/1‑94 (MQ=60)
               ATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTG                                                                                >  SRR4302537.493292/1‑94 (MQ=60)
               ATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTG                                                                                >  SRR4302537.5986018/1‑94 (MQ=60)
                TGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGT                                                                               >  SRR4302537.3767333/1‑94 (MQ=60)
                GGAGGCGGCACATTACGCTGATCCTCGCTCAGCGGACGGAGAAGGCGTTTACGAAAACCCAGTCGCCCATCCAGTCCGCCCGCCATCAGTTTGT                                                                               <  SRR4302537.3587174/94‑1 (MQ=60)
                 GGGGCGGCACATTACGCTAATCCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAACCCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTC                                                                              <  SRR4302537.4700506/94‑1 (MQ=60)
                 GAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTC                                                                              >  SRR4302537.2810756/1‑94 (MQ=60)
                    GCGGCACTTTCCGCTGATCCTCGCTCAGCGGACGGCGAAGGTGTTTACGTAAACCCAGTCGCGCATCCATTCCGCCCGCCATCAGTTTGTCGAT                                                                           <  SRR4302537.1201579/94‑1 (MQ=42)
                     CGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATG                                                                          >  SRR4302537.2169351/1‑94 (MQ=60)
                       gcatatgcCGCTGATCCTCGCGCGGCGGACGGCGAAGTCTTTTACGGAAAACCAGTCGCCCATCCGGTCCGCCCGCCATCAGTTTGTCGATGGT                                                                        <  SRR4302537.3554/86‑1 (MQ=28)
                        CACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTT                                                                       >  SRR4302537.813386/1‑94 (MQ=60)
                         cccttacgctaatcccCGCTCAGCGGAGGGAGAAGGCGTTTAGGGAAACCCAGTCGCGCACCCATTTCGCCCGCCATCAGTTTGTCGATGGTTT                                                                      <  SRR4302537.4325894/78‑1 (MQ=35)
                          CATTGCGCTGATCCTCGCCCAGCGGACGGCGAAGGTTTTTACGGTAACCCAGTCGCGCATCCAGTCCCCCCGCCATCAGTTTGTCGATGGTTTC                                                                     <  SRR4302537.6365801/94‑1 (MQ=60)
                           ATTACGCTGATACTCGCTGAGGGGAGGGCGAAGGGTTTTACGGAAAACCAGTCGCGCATCCATTTCCCCCGCCATCAGTTTGTCGATGGTTTCG                                                                    <  SRR4302537.5873775/94‑1 (MQ=60)
                             TACGCTGATACTCGCTCAGCGGACGGCGAGGGCTTTTACGGTAACCCAGTCGCCCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCG                                                                  <  SRR4302537.356389/94‑1 (MQ=60)
                             TACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCT                                                                  >  SRR4302537.1365815/1‑94 (MQ=60)
                               CGCTGATCCGCGCTCGGGGGAGGGCGAAGGCGTTTACGGTAACCCAGTCGCGCACCCAGTCCCCCCGCCATCAGTTTGTCGATGGTTTCGCGTA                                                                <  SRR4302537.5093560/94‑1 (MQ=60)
                               CGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTA                                                                >  SRR4302537.4562843/1‑94 (MQ=60)
                                GCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTAC                                                               >  SRR4302537.5360735/1‑94 (MQ=60)
                                GCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCATCAGTTTGTCGATGGTTTCGCGTAC                                                               <  SRR4302537.498342/94‑1 (MQ=60)
                                  TGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACAT                                                             >  SRR4302537.3928049/1‑94 (MQ=60)
                                    atcctcgcgcggcggggggggagggtGTTTACGGAAAACCGGTCGCGCACCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATAT                                                           <  SRR4302537.2824739/68‑1 (MQ=60)
                                    ATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATAT                                                           >  SRR4302537.3328747/1‑94 (MQ=60)
                                     tcccgcccgagggggcggcgaggctgtttacgtaaaaccggccgccCATCCAGTCCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATT                                                          <  SRR4302537.5798122/48‑1 (MQ=60)
                                      ACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTC                                                         >  SRR4302537.5846031/1‑94 (MQ=60)
                                       cccgcccgggggagggggaggggtTTTACGGTAACCCAGTCGCCCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCC                                                        <  SRR4302537.378090/70‑1 (MQ=60)
                                        cggcgcggcgggcggcaagggcgtttccgtaAAACCAGTCGCCCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCT                                                       <  SRR4302537.35001/63‑1 (MQ=60)
                                           ctcgaggcacggagaagactttcaggcgaaacaaccggagcaaaaagcgCACCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGAT                                                    <  SRR4302537.4515977/45‑1 (MQ=60)
                                             gagcgggcggagaaggctttgacgaaaccCCAGTCGCGCATCCAGTTCCCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATAT                                                  <  SRR4302537.647252/65‑1 (MQ=60)
                                             cggcggggggggagggctTTTACGGAAAACCGGGCGCGCATCCATTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATAT                                                  <  SRR4302537.5790344/76‑1 (MQ=60)
                                             CAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATAT                                                  >  SRR4302537.4632046/1‑94 (MQ=60)
                                              AGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATG                                                 >  SRR4302537.4430446/1‑94 (MQ=60)
                                              AGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATG                                                 >  SRR4302537.376579/1‑94 (MQ=60)
                                                 ggagggcgagggcttttaggataacccgctcccccacCCAGTTCCCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCT                                              <  SRR4302537.741435/57‑1 (MQ=60)
                                                     GGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTT                                          >  SRR4302537.4160194/1‑94 (MQ=60)
                                                     GGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTT                                          >  SRR4302537.927477/1‑94 (MQ=60)
                                                       CGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGC                                        >  SRR4302537.1301013/1‑94 (MQ=60)
                                                        gagtgtttttaggaaaacccgttcgcccacCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGCACATATTCCTGATATGGCTCGTTGCG                                       <  SRR4302537.3260058/64‑1 (MQ=60)
                                                        GAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGcttcg                                       >  SRR4302537.4150093/1‑89 (MQ=60)
                                                         AAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGG                                      >  SRR4302537.1003420/1‑94 (MQ=60)
                                                         AAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCCTTGCGG                                      >  SRR4302537.5803671/1‑94 (MQ=60)
                                                          AGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGGTGCCGA                                     >  SRR4302537.1455787/1‑94 (MQ=60)
                                                           GGCGTTTACGGTAAACCAGTCGCCCATCCAGTTCACCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAA                                    <  SRR4302537.4085597/94‑1 (MQ=60)
                                                            GCGTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCCTTGCGGGAG                                   >  SRR4302537.355566/1‑94 (MQ=60)
                                                             CGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGCTGCGGGAGA                                  >  SRR4302537.1230490/1‑94 (MQ=60)
                                                              GTTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGGAGAT                                 >  SRR4302537.5141794/1‑94 (MQ=60)
                                                               TTTACGGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGGTGCGGGAGATG                                >  SRR4302537.1528993/1‑94 (MQ=60)
                                                                TAACGGTAACCCAGTCGCCCACCCAGTCCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGC                               <  SRR4302537.5690615/94‑1 (MQ=60)
                                                                 tagggaaacccggcccCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCG                              <  SRR4302537.5667406/78‑1 (MQ=60)
                                                                   CGATACACCAGTCGCGCCTCCATTCCCCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCA                            <  SRR4302537.1912572/94‑1 (MQ=60)
                                                                    GGTAAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGGAGATGTGCAG                           >  SRR4302537.1856421/1‑94 (MQ=60)
                                                                     GAAAACCAGTCGCCCATCCAGTCCCCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGG                          <  SRR4302537.3554034/94‑1 (MQ=60)
                                                                       AAACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGGA                        >  SRR4302537.1415847/1‑94 (MQ=60)
                                                                        AACCAGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGGAT                       >  SRR4302537.3338907/1‑94 (MQ=60)
                                                                            AGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGGAGAAGTGCAGGTATAGAT                   >  SRR4302537.6283047/1‑94 (MQ=60)
                                                                            AGTCGCGCATCCAGTTCGCCCGCCATCAGTTTGTCGATGGCTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGAGCAGGTATAGCT                   >  SRR4302537.3396207/1‑94 (MQ=60)
                                                                                  CCATCCTGTTCCCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATAGCTCCTGCT             <  SRR4302537.1859616/94‑1 (MQ=60)
                                                                                   CATCCAGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGGATAGGTCCCGCTG            >  SRR4302537.360743/1‑94 (MQ=60)
                                                                                       CAGTCCCCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATAGCTCCTGCTGAAAC        <  SRR4302537.2622586/94‑1 (MQ=60)
                                                                                        AGTTCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGCAGATGCGCAGGTATAGCTCCTGCTGAAACT       >  SRR4302537.378848/1‑94 (MQ=60)
                                                                                         GTTCACCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGGATAGCTTCTGCTGAAACTG      >  SRR4302537.3655732/1‑94 (MQ=60)
                                                                                           TCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATAGCTCCTGCTGAAACTGCT    <  SRR4302537.4761343/94‑1 (MQ=60)
                                                                                           TCGCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATAGCTCCTGCTGAAACTGCT    <  SRR4302537.6027015/94‑1 (MQ=60)
                                                                                             GCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGGAGATGCGGCGGGATAGCTCCTGCTGAAACTGCTGG  >  SRR4302537.4709113/1‑94 (MQ=60)
                                                                                             GCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATAGCTCCTGCTGAAACTGCTGG  >  SRR4302537.1674535/1‑94 (MQ=60)
                                                                                             CCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATAGCTCCTGCTGAAACTGCTGG  <  SRR4302537.2142467/94‑1 (MQ=60)
                                                                                             CCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATAGCTCCTGCTGAAACTGCTGG  <  SRR4302537.4224796/94‑1 (MQ=60)
                                                                                             CCCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATAGCTCCTGCTGAAACTGCTGG  <  SRR4302537.853902/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCATCAGTTTGTCGATGGTTTCGCGTACATATTCCTGATATGGCTCGTTGCGGAAGATGCGCAGGTATAGCTCCTGCTGAAACTGCTGG  >  NC_000913/63660‑63846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑