Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	312,219	T→C	intergenic (‑218/‑1362)	ykgM ← / → eaeH	putative ribosomal protein/attaching and effacing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	312,219	0	T	C	100.0%	846.6 / NA	240	intergenic (‑218/‑1362)	ykgM/eaeH	putative ribosomal protein/attaching and effacing protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (114/126); total (114/126)

Only 100 of 242 total aligned reads displayed.

CAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAA  >  NC_000913/312129‑312308

  gcCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCAc                                                                                       >  2:3263075/1‑93 (MQ=255)

  gcCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCAc                                                                                       >  2:2723682/1‑93 (MQ=255)

  gcCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCAc                                                                                       <  2:4940487/93‑1 (MQ=255)

   ccATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACt                                                                                      >  1:983355/1‑93 (MQ=255)

   ccATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACt                                                                                      >  1:1794395/1‑93 (MQ=255)

    cATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACtt                                                                                     >  1:6223901/1‑93 (MQ=255)

     aTTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACttt                                                                                    >  1:523700/1‑93 (MQ=255)

      ttGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTa                                                                                   >  1:4497243/1‑93 (MQ=255)

      ttGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTa                                                                                   <  2:6251946/93‑1 (MQ=255)

       tGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTAc                                                                                  <  2:1338642/93‑1 (MQ=255)

        ggTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACt                                                                                 <  1:3512510/93‑1 (MQ=255)

        ggTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACt                                                                                 >  2:5184602/1‑93 (MQ=255)

        ggTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACt                                                                                 <  1:6263628/93‑1 (MQ=255)

        ggTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTGTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACt                                                                                 <  2:5153173/93‑1 (MQ=255)

           gACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGc                                                                              >  1:5386380/1‑93 (MQ=255)

            aCGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGcc                                                                             >  2:4852093/1‑93 (MQ=255)

               ctaccATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGAt                                                                          >  2:882467/4‑93 (MQ=255)

                tCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATa                                                                         >  1:3264872/1‑93 (MQ=255)

                tCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATa                                                                         <  1:5058204/93‑1 (MQ=255)

                tCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATa                                                                         <  1:4404579/93‑1 (MQ=255)

                 cccATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATaa                                                                        <  2:167245/93‑1 (MQ=255)

                 cccATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATaa                                                                        >  1:3740883/1‑93 (MQ=255)

                 cccATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATaa                                                                        >  2:3188485/1‑93 (MQ=255)

                  ccATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAAt                                                                       >  2:2768896/1‑93 (MQ=255)

                     tAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTa                                                                    <  2:191558/93‑1 (MQ=255)

                     tAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTa                                                                    >  1:6156973/1‑93 (MQ=255)

                     tAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTa                                                                    <  1:1502355/93‑1 (MQ=255)

                     tAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTa                                                                    >  1:5188042/1‑93 (MQ=255)

                       aaCGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATc                                                                  <  2:2810490/93‑1 (MQ=255)

                       aaCGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATc                                                                  >  2:251345/1‑93 (MQ=255)

                          gCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCa                                                               >  1:4961106/1‑93 (MQ=255)

                           ccTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAg                                                              <  2:4166061/93‑1 (MQ=255)

                            cTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGt                                                             <  2:2091206/93‑1 (MQ=255)

                             tGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTa                                                            >  1:2293106/1‑93 (MQ=255)

                             tGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTa                                                            <  2:2079509/93‑1 (MQ=255)

                                aTGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTAtga                                                         <  2:3066106/93‑1 (MQ=255)

                                 tGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTAtgat                                                        <  1:2813490/93‑1 (MQ=255)

                                 tGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTAtgat                                                        >  1:3076973/1‑93 (MQ=255)

                                     tgattgatGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTAtgattgat                                                    <  1:4562118/93‑1 (MQ=255)

                                       attgatGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTa                                                  <  2:2596777/93‑1 (MQ=255)

                                        ttgatTATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTAc                                                 <  2:1508079/93‑1 (MQ=255)

                                        ttgatGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTAc                                                 <  1:1726965/93‑1 (MQ=255)

                                         tgatgaTGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTAcc                                                <  1:4300708/93‑1 (MQ=255)

                                          gatgatGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCt                                               <  1:3042652/93‑1 (MQ=255)

                                          gatgatGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCt                                               <  1:1540859/93‑1 (MQ=255)

                                          gatgatGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCt                                               >  1:3837708/1‑93 (MQ=255)

                                           atgatgGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTg                                              >  1:1890263/1‑93 (MQ=255)

                                            tgatgGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGa                                             <  2:4343370/93‑1 (MQ=255)

                                               tgGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAg                                          >  1:3692369/1‑93 (MQ=255)

                                               tgGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAg                                          <  2:2271341/93‑1 (MQ=255)

                                                     tGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTa                                    <  2:1099960/93‑1 (MQ=255)

                                                      gCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTAt                                   <  1:1625636/93‑1 (MQ=255)

                                                       cTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTAtt                                  >  2:4655615/1‑93 (MQ=255)

                                                       cTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTAtt                                  <  1:4257972/93‑1 (MQ=255)

                                                        tGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTc                                 >  1:2280599/1‑93 (MQ=255)

                                                        tGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTc                                 >  2:2281545/1‑93 (MQ=255)

                                                        tGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTc                                 >  1:2686263/1‑93 (MQ=255)

                                                         gagaGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTcc                                <  2:3620897/93‑1 (MQ=255)

                                                         gagaGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTcc                                >  2:3996297/1‑93 (MQ=255)

                                                         gagaGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTcc                                <  1:3406620/93‑1 (MQ=255)

                                                          agagTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCct                               <  2:6143883/93‑1 (MQ=255)

                                                           gagTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCctc                              <  2:4462736/93‑1 (MQ=255)

                                                            agTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCctct                             <  1:4362965/93‑1 (MQ=255)

                                                            agTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCctct                             >  1:5605061/1‑93 (MQ=255)

                                                             gTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCtctc                            >  2:1802495/1‑93 (MQ=255)

                                                              tATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTcc                           >  1:2750151/1‑93 (MQ=255)

                                                              tATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTcc                           >  2:3028552/1‑93 (MQ=255)

                                                              tATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTcc                           >  2:1981519/1‑93 (MQ=255)

                                                                tGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCa                         >  1:2860083/1‑93 (MQ=255)

                                                                tGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCa                         >  2:338332/1‑93 (MQ=255)

                                                                 ggATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCAc                        >  2:3737132/1‑93 (MQ=255)

                                                                 ggATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCAc                        <  2:3921948/93‑1 (MQ=255)

                                                                  gATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACg                       <  1:6111153/93‑1 (MQ=255)

                                                                  gATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACg                       >  1:1063450/1‑93 (MQ=255)

                                                                   aTTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACgg                      <  1:5415591/93‑1 (MQ=255)

                                                                    tttttCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGa                     <  2:5097646/93‑1 (MQ=255)

                                                                    tttttCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGa                     >  2:5695758/1‑93 (MQ=255)

                                                                    tttttCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGa                     <  2:6007757/93‑1 (MQ=255)

                                                                    tttttCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGa                     >  2:1185184/1‑93 (MQ=255)

                                                                    tttttCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGa                     <  1:2869638/93‑1 (MQ=255)

                                                                    tttttCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGa                     >  2:1418350/1‑93 (MQ=255)

                                                                     ttttCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGat                    <  2:2395969/93‑1 (MQ=255)

                                                                     ttttCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGat                    <  1:691905/93‑1 (MQ=255)

                                                                      tttCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGata                   <  2:153892/93‑1 (MQ=255)

                                                                      tttCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGata                   >  2:5135674/1‑93 (MQ=255)

                                                                      tttCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGata                   <  2:538802/93‑1 (MQ=255)

                                                                        tCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGAtata                 >  1:5341348/1‑93 (MQ=255)

                                                                        tCGAGAATGAAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGAtata                 >  1:3946745/1‑93 (MQ=255)

                                                                          gagaATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATataat               <  2:5018975/93‑1 (MQ=255)

                                                                          gagaATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATataat               >  1:5559383/1‑93 (MQ=255)

                                                                          gagaATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATataat               >  1:4758285/1‑93 (MQ=255)

                                                                              aTGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAAtt           >  2:695888/1‑93 (MQ=255)

                                                                               tGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAAtta          >  1:6353401/1‑93 (MQ=255)

                                                                               tGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAAtta          >  2:529494/1‑93 (MQ=255)

                                                                               tGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAAtta          >  1:4027073/1‑93 (MQ=255)

                                                                                    gTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAAttacttac     <  1:1375166/93‑1 (MQ=255)

                                                                                    gTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAAttacttac     <  2:471918/93‑1 (MQ=255)

                                                                                     ttATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACa    >  2:1693563/1‑93 (MQ=255)

                                                                                       aTCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACaaa  <  2:5803523/93‑1 (MQ=255)

                                                                                       aTCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACaaa  >  1:5771501/1‑93 (MQ=255)

CAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAA  >  NC_000913/312129‑312308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAG  >  NC_000913/312126‑312312

TAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCC                                                                                               <  SRR4302537.5940237/94‑1 (MQ=60)

TAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCC                                                                                               >  SRR4302537.4168239/1‑94 (MQ=60)

 AGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCC                                                                                              <  SRR4302537.4514378/94‑1 (MQ=60)

 AGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCC                                                                                              <  SRR4302537.371668/94‑1 (MQ=60)

   CAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCA                                                                                            >  SRR4302537.1515318/1‑94 (MQ=60)

     GCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACT                                                                                          >  SRR4302537.990148/1‑94 (MQ=60)

     GCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACT                                                                                          >  SRR4302537.1806915/1‑94 (MQ=60)

      CCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTT                                                                                         >  SRR4302537.1652238/1‑94 (MQ=60)

      CCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTT                                                                                         >  SRR4302537.6266737/1‑94 (MQ=60)

       CATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTT                                                                                        >  SRR4302537.527294/1‑94 (MQ=60)

        ATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTA                                                                                       >  SRR4302537.3446763/1‑94 (MQ=60)

        ATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTA                                                                                       >  SRR4302537.4528353/1‑94 (MQ=60)

        ATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTA                                                                                       >  SRR4302537.6304626/1‑94 (MQ=60)

          TGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACT                                                                                     <  SRR4302537.1859075/94‑1 (MQ=60)

           GGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTG                                                                                    <  SRR4302537.3537358/94‑1 (MQ=60)

           GGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTG                                                                                    <  SRR4302537.6306625/94‑1 (MQ=60)

             TGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGC                                                                                  >  SRR4302537.5423197/1‑94 (MQ=60)

                CGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGA                                                                               >  SRR4302537.1743454/1‑94 (MQ=60)

                CGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGA                                                                               <  SRR4302537.3022924/94‑1 (MQ=60)

                 GCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGAT                                                                              <  SRR4302537.3344597/94‑1 (MQ=60)

                  CTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATA                                                                             >  SRR4302537.3287902/1‑94 (MQ=60)

                   TCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAA                                                                            <  SRR4302537.4434970/94‑1 (MQ=60)

                   TCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAA                                                                            <  SRR4302537.5093023/94‑1 (MQ=60)

                   TCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAA                                                                            >  SRR4302537.3767156/1‑94 (MQ=60)

                    CCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAAT                                                                           >  SRR4302537.4014721/1‑94 (MQ=60)

                     CCATAAACGTCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACGTTACTGGCCGATAATT                                                                          >  SRR4302537.4328933/1‑94 (MQ=60)

                     CCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATT                                                                          >  SRR4302537.2865844/1‑94 (MQ=60)

                     CCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATT                                                                          <  SRR4302537.3085016/94‑1 (MQ=60)

                      CATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTT                                                                         <  SRR4302537.5331082/94‑1 (MQ=60)

                       ATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTA                                                                        >  SRR4302537.6199343/1‑94 (MQ=60)

                       ATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTA                                                                        >  SRR4302537.5223722/1‑94 (MQ=60)

                        TAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTAT                                                                       <  SRR4302537.1512844/94‑1 (MQ=60)

                        TAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTAT                                                                       <  SRR4302537.2819376/94‑1 (MQ=60)

                        TAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTAT                                                                       >  SRR4302537.5436795/1‑94 (MQ=60)

                         AAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATC                                                                      <  SRR4302537.443551/94‑1 (MQ=60)

                            CGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCA                                                                   >  SRR4302537.4995305/1‑94 (MQ=60)

                             GCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAG                                                                  >  SRR4302537.5006363/1‑94 (MQ=60)

                             GCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAG                                                                  <  SRR4302537.975787/94‑1 (MQ=60)

                              CCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGT                                                                 >  SRR4302537.5105795/1‑94 (MQ=60)

                              CCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGT                                                                 <  SRR4302537.5623368/94‑1 (MQ=60)

                               CTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTA                                                                >  SRR4302537.2309292/1‑94 (MQ=60)

                                 GGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATG                                                              <  SRR4302537.6099063/94‑1 (MQ=60)

                                   ATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGAT                                                            >  SRR4302537.3098408/1‑94 (MQ=60)

                                    TGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATT                                                           <  SRR4302537.2833164/94‑1 (MQ=60)

                                     GAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTG                                                          <  SRR4302537.2537115/94‑1 (MQ=60)

                                     GAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTG                                                          >  SRR4302537.1595063/1‑94 (MQ=60)

                                     GAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTG                                                          <  SRR4302537.3721168/94‑1 (MQ=60)

                                        TGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATT                                                       <  SRR4302537.4593699/94‑1 (MQ=60)

                                        TGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATT                                                       <  SRR4302537.4555234/94‑1 (MQ=60)

                                        TGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATT                                                       <  SRR4302537.3818169/94‑1 (MQ=60)

                                           TTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACC                                                    >  SRR4302537.3183524/1‑94 (MQ=60)

                                           TTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACC                                                    <  SRR4302537.1739029/94‑1 (MQ=60)

                                            TGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCT                                                   <  SRR4302537.4773783/94‑1 (MQ=60)

                                            TGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCT                                                   >  SRR4302537.3864593/1‑94 (MQ=60)

                                            TGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCT                                                   <  SRR4302537.4330598/94‑1 (MQ=60)

                                            TGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCT                                                   <  SRR4302537.3243390/94‑1 (MQ=60)

                                             GATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTG                                                  <  SRR4302537.1551613/94‑1 (MQ=60)

                                             GATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTG                                                  <  SRR4302537.3063851/94‑1 (MQ=60)

                                             GATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTG                                                  >  SRR4302537.1903401/1‑94 (MQ=60)

                                                GATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACCGGCCGATAATTTATCGCAGTATGATTGATTACCTGACA                                               >  SRR4302537.3633510/1‑94 (MQ=60)

                                                 ATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAG                                              >  SRR4302537.3718354/1‑94 (MQ=60)

                                                  TGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGC                                             >  SRR4302537.3982738/1‑94 (MQ=60)

                                                      TTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGT                                         >  SRR4302537.1530444/1‑94 (MQ=60)

                                                        TGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTAT                                       <  SRR4302537.69561/94‑1 (MQ=60)

                                                        TGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTAT                                       >  SRR4302537.4447183/1‑94 (MQ=60)

                                                        TGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTAT                                       <  SRR4302537.618764/94‑1 (MQ=60)

                                                        TGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTAT                                       <  SRR4302537.4428900/94‑1 (MQ=60)

                                                         GCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATT                                      <  SRR4302537.1637006/94‑1 (MQ=60)

                                                          CTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTC                                     >  SRR4302537.2296693/1‑94 (MQ=60)

                                                          CTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTC                                     >  SRR4302537.2705012/1‑94 (MQ=60)

                                                          CTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTC                                     <  SRR4302537.4287562/94‑1 (MQ=60)

                                                          CTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTC                                     >  SRR4302537.2304601/1‑94 (MQ=60)

                                                           TGAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCC                                    <  SRR4302537.6187540/94‑1 (MQ=60)

                                                            GAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCT                                   <  SRR4302537.2665861/94‑1 (MQ=60)

                                                            GAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCT                                   <  SRR4302537.3430751/94‑1 (MQ=60)

                                                            GAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCT                                   <  SRR4302537.4839428/94‑1 (MQ=60)

                                                            GAGAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCT                                   <  SRR4302537.3779866/94‑1 (MQ=60)

                                                              GAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCT                                 >  SRR4302537.5643382/1‑94 (MQ=60)

                                                              GAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCT                                 <  SRR4302537.4743954/94‑1 (MQ=60)

                                                              GAGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCT                                 <  SRR4302537.2976449/94‑1 (MQ=60)

                                                               AGTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTC                                <  SRR4302537.4393144/94‑1 (MQ=60)

                                                                GTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCC                               <  SRR4302537.5474630/94‑1 (MQ=60)

                                                                GTATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCC                               >  SRR4302537.2769339/1‑94 (MQ=60)

                                                                 TATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCC                              >  SRR4302537.1133146/1‑94 (MQ=60)

                                                                  ATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCA                             >  SRR4302537.149387/1‑94 (MQ=60)

                                                                  ATGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCA                             >  SRR4302537.2880137/1‑94 (MQ=60)

                                                                   TGGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCAC                            <  SRR4302537.4258786/94‑1 (MQ=60)

                                                                    GGATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACG                           >  SRR4302537.1070778/1‑94 (MQ=60)

                                                                     GATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGG                          <  SRR4302537.6153149/94‑1 (MQ=60)

                                                                      ATTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGA                         <  SRR4302537.5452513/94‑1 (MQ=60)

                                                                       TTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGAT                        >  SRR4302537.5705209/1‑94 (MQ=60)

                                                                       TTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGAT                        <  SRR4302537.2889779/94‑1 (MQ=60)

                                                                       TTTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGAT                        >  SRR4302537.5418761/1‑94 (MQ=60)

                                                                        TTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATA                       <  SRR4302537.696642/94‑1 (MQ=60)

                                                                        TTTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATA                       >  SRR4302537.16679/1‑94 (MQ=60)

                                                                         TTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATAT                      <  SRR4302537.2140030/94‑1 (MQ=60)

                                                                         TTTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATAT                      <  SRR4302537.572616/94‑1 (MQ=60)

                                                                          TTCGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATA                     >  SRR4302537.5377955/1‑94 (MQ=60)

                                                                          TTCGAGAATGAAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATA                     >  SRR4302537.3974350/1‑94 (MQ=60)

                                                                            CGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAAT                   >  SRR4302537.4791149/1‑94 (MQ=60)

                                                                            CGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAAT                   <  SRR4302537.2020794/94‑1 (MQ=60)

                                                                            CGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAAT                   >  SRR4302537.5597372/1‑94 (MQ=60)

                                                                            CGAGAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAAT                   <  SRR4302537.5225713/94‑1 (MQ=60)

                                                                              AGAATGGAGGTTATCCCCACTGTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAA                 <  SRR4302537.6308565/94‑1 (MQ=60)

                                                                               GAATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAAT                <  SRR4302537.5345968/94‑1 (MQ=60)

                                                                                AATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATT               >  SRR4302537.6219747/1‑94 (MQ=60)

                                                                                 ATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTA              >  SRR4302537.6396773/1‑94 (MQ=60)

                                                                                 ATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTA              <  SRR4302537.4355352/94‑1 (MQ=60)

                                                                                 ATGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTA              >  SRR4302537.4055266/1‑94 (MQ=60)

                                                                                  TGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTAC             <  SRR4302537.5553245/94‑1 (MQ=60)

                                                                                  TGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTAC             <  SRR4302537.2885179/94‑1 (MQ=60)

                                                                                  TGGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTAC             >  SRR4302537.3967448/1‑94 (MQ=60)

                                                                                   GGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACT            <  SRR4302537.888537/94‑1 (MQ=60)

                                                                                   GGAGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACT            <  SRR4302537.2762664/94‑1 (MQ=60)

                                                                                     AGGTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTA          >  SRR4302537.4375400/1‑94 (MQ=60)

                                                                                       GTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACA        <  SRR4302537.3636189/94‑1 (MQ=60)

                                                                                       GTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACA        <  SRR4302537.1384765/94‑1 (MQ=60)

                                                                                       GTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACA        >  SRR4302537.6389419/1‑94 (MQ=60)

                                                                                       GTTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACA        >  SRR4302537.5214002/1‑94 (MQ=60)

                                                                                        TTATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAA       <  SRR4302537.2788244/94‑1 (MQ=60)

                                                                                         TATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAA      >  SRR4302537.5811060/1‑94 (MQ=60)

                                                                                          ATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAA     >  SRR4302537.3774668/1‑94 (MQ=60)

                                                                                          ATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAA     >  SRR4302537.5751966/1‑94 (MQ=60)

                                                                                          ATCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAA     >  SRR4302537.3394693/1‑94 (MQ=60)

                                                                                           TCCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAA    >  SRR4302537.5943289/1‑94 (MQ=60)

                                                                                            CCCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAA   <  SRR4302537.2320706/94‑1 (MQ=60)

                                                                                             CCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAG  >  SRR4302537.518362/1‑94 (MQ=60)

                                                                                             CCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAG  <  SRR4302537.4984035/94‑1 (MQ=60)

                                                                                             CCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAG  <  SRR4302537.5281313/94‑1 (MQ=60)

TAGCAGCCATTGGTGACGCTCCCATAAACGCCTGGATGAGTGATTGATGATGGTTTTGCTGAGAGTATGGATTTTTCGAGAATGGAGGTTATCTCCACTTTACTGGCCGATAATTTATCGCAGTATGATTGATTACCTGACAGCGTGTATTCCTCTCCCACGGATATAATAATTACTTACAAAAAAG  >  NC_000913/312126‑312312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`