Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 337,988 C→T P147L (CCG→CTG)  yahG → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913337,9880CT99.5% 744.4 / ‑5.2 208P147L (CCG→CTG) yahGhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (90/117);  minor base A (1/0);  total (91/117)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 223 total aligned reads displayed.

GGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGGC  >  NC_000913/337899‑338076
                                                                                         |                                                                                        
 gTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTgtgt                                                                                      <  1:3140306/93‑1 (MQ=255)
 gTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTgtgt                                                                                      <  2:4434182/93‑1 (MQ=255)
  tCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTc                                                                                     >  1:4093418/1‑93 (MQ=255)
      cGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGa                                                                                 <  1:5945775/93‑1 (MQ=255)
      cGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGa                                                                                 <  1:1783527/93‑1 (MQ=255)
       ggAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAg                                                                                >  1:69391/1‑93 (MQ=255)
          gcgcTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCAc                                                                             <  1:1489258/93‑1 (MQ=255)
            gcTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGa                                                                           >  1:1980169/1‑93 (MQ=255)
             cTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGAc                                                                          <  1:616971/93‑1 (MQ=255)
             cTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGAc                                                                          >  2:696080/1‑93 (MQ=255)
              tGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACt                                                                         >  1:2046521/1‑93 (MQ=255)
              tGGTGTTCGAAGGACTGGCGAAAGAGCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACt                                                                         <  1:3117159/93‑1 (MQ=255)
               ggTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTg                                                                        <  2:5248767/93‑1 (MQ=255)
                gtgtTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGTAGATCACCTTCTCGCTGTGTCACGAGCACGACTGc                                                                       <  2:4398909/93‑1 (MQ=255)
                gtgtTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGc                                                                       <  1:5520136/93‑1 (MQ=255)
                gtgtTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGAACAACTTCTTGCTGTGTCACGAGCACGACTGc                                                                       >  1:2087838/1‑93 (MQ=255)
                  gtTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCgg                                                                     >  1:5647496/1‑92 (MQ=255)
                    tCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTgg                                                                   >  2:150977/1‑93 (MQ=255)
                    tCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTgg                                                                   <  1:4006362/93‑1 (MQ=255)
                    tCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTgg                                                                   <  1:4292986/93‑1 (MQ=255)
                    tCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTgg                                                                   >  2:5947682/1‑93 (MQ=255)
                      gAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGa                                                                 <  2:1942092/93‑1 (MQ=255)
                      gAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGa                                                                 >  1:5189427/1‑93 (MQ=255)
                       aaGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGAt                                                                <  2:1586191/93‑1 (MQ=255)
                       aaGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGAt                                                                <  2:1239240/93‑1 (MQ=255)
                        aGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATc                                                               >  1:2517398/1‑93 (MQ=255)
                        aGGACTGGCGAAAGATCTCGACGAGCCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATc                                                               <  2:1996968/93‑1 (MQ=255)
                         ggACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCg                                                              <  2:1857765/93‑1 (MQ=255)
                          gACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGa                                                             <  1:3816010/93‑1 (MQ=255)
                          gACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGa                                                             >  2:3511479/1‑93 (MQ=255)
                          gACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGa                                                             >  1:5573777/1‑93 (MQ=255)
                          gACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGa                                                             >  2:4907967/1‑93 (MQ=255)
                          gACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGa                                                             >  1:1115279/1‑93 (MQ=255)
                           aCTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGAt                                                            >  2:4840099/1‑93 (MQ=255)
                           aCTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGAt                                                            <  1:5638113/93‑1 (MQ=255)
                            cTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATg                                                           <  2:1129213/93‑1 (MQ=255)
                            cTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATg                                                           >  2:835226/1‑93 (MQ=255)
                            cTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATg                                                           >  1:2234826/1‑93 (MQ=255)
                              ggCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGc                                                         >  1:3288551/1‑93 (MQ=255)
                                cGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCgg                                                       <  2:2987571/93‑1 (MQ=255)
                                 gAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCggg                                                      >  2:2073204/1‑93 (MQ=255)
                                  aaaGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGgt                                                     >  2:4591500/1‑93 (MQ=255)
                                  aaaGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGgt                                                     >  1:919958/1‑93 (MQ=255)
                                   aaGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGgtg                                                    <  2:4941447/93‑1 (MQ=255)
                                      aTCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTa                                                 <  1:2649342/93‑1 (MQ=255)
                                      aTCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTa                                                 <  2:22656/93‑1 (MQ=255)
                                          cgacgaGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTc                                             <  1:1635026/93‑1 (MQ=255)
                                          cgacgaGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTc                                             <  2:3745848/93‑1 (MQ=255)
                                           gacgaGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCg                                            <  2:3082110/93‑1 (MQ=255)
                                            acgaGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCgg                                           >  1:4732619/1‑93 (MQ=255)
                                             cgaGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCCCGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGc                                          <  1:2680469/93‑1 (MQ=255)
                                              gaGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGcc                                         <  1:4924266/93‑1 (MQ=255)
                                               aGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCt                                        >  1:3197200/1‑93 (MQ=255)
                                               aGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCt                                        <  1:4541211/93‑1 (MQ=255)
                                                ggcggcTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTc                                       <  2:3451120/93‑1 (MQ=255)
                                                 gcggcTGAACTGGCGGCTTCCCGGGAGATCACCTTCTCGCTGTGTCACGAGCGCGACTGGGTGGGATCCAGTGCGGGTGTTACCTCGGCCTCg                                      >  2:3308159/1‑93 (MQ=255)
                                                  cggcTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGa                                     <  1:3719926/93‑1 (MQ=255)
                                                  cggcTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGa                                     <  2:2819830/93‑1 (MQ=255)
                                                    gcTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATg                                   >  2:5960295/1‑93 (MQ=255)
                                                     cTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGt                                  <  2:2462446/93‑1 (MQ=255)
                                                     cTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGt                                  >  2:5356849/1‑93 (MQ=255)
                                                      tGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGtt                                 <  2:1091776/93‑1 (MQ=255)
                                                      tGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGtt                                 >  1:219588/1‑93 (MQ=255)
                                                        aaCTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTa                               <  2:4103369/93‑1 (MQ=255)
                                                        aaCTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTa                               >  2:5823038/1‑93 (MQ=255)
                                                        aaCTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTa                               <  1:4144253/93‑1 (MQ=255)
                                                          cTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATg                             >  1:3767356/1‑93 (MQ=255)
                                                            ggcggcTTCCGGGGAGATCACCTTCTCGCTGTGTCACTAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGca                           <  1:6436482/93‑1 (MQ=255)
                                                             gcggcTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGcac                          >  1:2300566/1‑93 (MQ=255)
                                                             gcggcTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGcac                          <  1:3569116/93‑1 (MQ=255)
                                                             gcggcTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGcac                          >  1:1716284/1‑93 (MQ=255)
                                                             gcggcTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGcac                          <  2:3388417/93‑1 (MQ=255)
                                                              cggcTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGcaca                         <  1:1227848/93‑1 (MQ=255)
                                                               ggcTTCCGGGGAGATCGCCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACAt                        <  2:4496058/93‑1 (MQ=255)
                                                               ggcTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCCATGCTTATGCACAt                        >  2:6371805/1‑93 (MQ=255)
                                                                  ttCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGt                     >  1:1901515/1‑93 (MQ=255)
                                                                      ggggAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTaaaa                 >  1:6019242/1‑93 (MQ=255)
                                                                      ggggAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTaaaa                 <  2:5649695/93‑1 (MQ=255)
                                                                       gggAGATCTCCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTaaaaa                <  1:982546/93‑1 (MQ=255)
                                                                       gggAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTaaaaa                <  1:3000113/93‑1 (MQ=255)
                                                                       gggAGATCACCTTCTCGCTGTGTCACAAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTaaaaa                >  1:246859/1‑93 (MQ=255)
                                                                        ggAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTaaaaaa               <  1:6358043/93‑1 (MQ=255)
                                                                         gagaTCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAaaaac              <  1:6401706/93‑1 (MQ=255)
                                                                         gagaTCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAaaaac              >  2:2304539/1‑93 (MQ=255)
                                                                         gagaTCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAaaaac              >  2:2654610/1‑93 (MQ=255)
                                                                         gagaTCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAaaaac              <  1:5018892/93‑1 (MQ=255)
                                                                           gaTCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAaaaacaa            <  2:4194747/93‑1 (MQ=255)
                                                                           gaTCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAaaaacaa            >  1:29359/1‑93 (MQ=255)
                                                                            aTCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAaaaacaaa           <  1:3334007/93‑1 (MQ=255)
                                                                            aTCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAaaaacaaa           >  2:6421091/1‑93 (MQ=255)
                                                                              cACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAaaaacaaaac         >  2:6080434/1‑93 (MQ=255)
                                                                               cccTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAAcc        <  1:794054/92‑1 (MQ=255)
                                                                               aCCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAAcc        >  1:1293108/1‑93 (MQ=255)
                                                                                ccTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCt       <  1:4982983/93‑1 (MQ=255)
                                                                                 cTTCTCGCTGTTACACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTa      <  2:6026989/93‑1 (MQ=255)
                                                                                 cTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTa      >  2:5487832/1‑93 (MQ=255)
                                                                                 cTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTa      >  2:1308291/1‑93 (MQ=255)
                                                                                  ttCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTAc     <  2:5046941/93‑1 (MQ=255)
                                                                                  ttCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTAc     <  2:3667922/93‑1 (MQ=255)
                                                                                     tcGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGGc  >  1:4371065/1‑93 (MQ=255)
                                                                                         |                                                                                        
GGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGGC  >  NC_000913/337899‑338076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGGCAACAT  >  NC_000913/337895‑338081
                                                                                             |                                                                                             
GCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCCCCTTCTCGCT                                                                                               <  SRR4302537.5138247/94‑1 (MQ=60)
 CGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTG                                                                                              >  SRR4302537.2670191/1‑94 (MQ=60)
   CGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTG                                                                                            >  SRR4302537.3826801/1‑94 (MQ=60)
     GTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTC                                                                                          <  SRR4302537.3162346/94‑1 (MQ=60)
     GTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTC                                                                                          >  SRR4302537.4122007/1‑94 (MQ=60)
       CACAGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCGCCTTCTCGCTGTGTCAC                                                                                        <  SRR4302537.159901/94‑1 (MQ=60)
        ACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACG                                                                                       <  SRR4302537.3170424/94‑1 (MQ=60)
         CCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGA                                                                                      <  SRR4302537.5603523/94‑1 (MQ=60)
          CGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAG                                                                                     <  SRR4302537.1795964/94‑1 (MQ=60)
          CGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAG                                                                                     >  SRR4302537.69898/1‑94 (MQ=60)
          CGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAG                                                                                     <  SRR4302537.5986585/94‑1 (MQ=60)
           GGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGC                                                                                    >  SRR4302537.2250097/1‑94 (MQ=60)
            GAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCA                                                                                   <  SRR4302537.4169362/94‑1 (MQ=60)
             AGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCAC                                                                                  >  SRR4302537.2040355/1‑94 (MQ=60)
              GCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACG                                                                                 <  SRR4302537.1499623/94‑1 (MQ=60)
              GCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACG                                                                                 <  SRR4302537.763600/94‑1 (MQ=60)
               CGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGA                                                                                >  SRR4302537.89994/1‑94 (MQ=60)
               CGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGA                                                                                >  SRR4302537.1993928/1‑94 (MQ=60)
                GCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTTGCTGTGTCACGAGCACGAC                                                                               >  SRR4302537.1786887/1‑94 (MQ=60)
                 CTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACT                                                                              <  SRR4302537.621172/94‑1 (MQ=60)
                 CTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACT                                                                              >  SRR4302537.2060867/1‑94 (MQ=60)
                 CTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACT                                                                              <  SRR4302537.1854963/94‑1 (MQ=60)
                  TGGTGTTCGAAGGACTGGCGAAAGAGCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTG                                                                             <  SRR4302537.3138930/94‑1 (MQ=60)
                   GGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGAACAACTTCTTGCTGTGTCACGAGCACGACTGC                                                                            >  SRR4302537.2102565/1‑94 (MQ=60)
                    GTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCG                                                                           <  SRR4302537.5557823/94‑1 (MQ=60)
                    GTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCG                                                                           >  SRR4302537.3168151/1‑94 (MQ=60)
                     TGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGG                                                                          >  SRR4302537.267342/1‑94 (MQ=60)
                     TGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGG                                                                          >  SRR4302537.5686150/1‑94 (MQ=60)
                      GTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTG                                                                         >  SRR4302537.2158337/1‑94 (MQ=60)
                      GTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTG                                                                         >  SRR4302537.295090/1‑94 (MQ=60)
                      GTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTG                                                                         <  SRR4302537.3387376/94‑1 (MQ=60)
                      GTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTG                                                                         <  SRR4302537.3153113/94‑1 (MQ=60)
                       TTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGG                                                                        >  SRR4302537.98689/1‑94 (MQ=60)
                        TCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGG                                                                       <  SRR4302537.4322826/94‑1 (MQ=60)
                        TCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGG                                                                       <  SRR4302537.4034400/94‑1 (MQ=60)
                         CGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGA                                                                      >  SRR4302537.5225120/1‑94 (MQ=60)
                         CGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGA                                                                      >  SRR4302537.6413390/1‑94 (MQ=60)
                           AAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATC                                                                    >  SRR4302537.2535087/1‑94 (MQ=60)
                            AGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCG                                                                   <  SRR4302537.4850600/94‑1 (MQ=60)
                            AGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCG                                                                   <  SRR4302537.5680147/94‑1 (MQ=60)
                             GGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGA                                                                  >  SRR4302537.5611875/1‑94 (MQ=60)
                             GGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGA                                                                  >  SRR4302537.1122975/1‑94 (MQ=60)
                              GACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGAT                                                                 <  SRR4302537.1916505/94‑1 (MQ=60)
                              GACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGAT                                                                 <  SRR4302537.3842774/94‑1 (MQ=60)
                               ACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATG                                                                <  SRR4302537.3777264/94‑1 (MQ=60)
                               ACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATG                                                                >  SRR4302537.2250588/1‑94 (MQ=60)
                               ACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATG                                                                <  SRR4302537.5676697/94‑1 (MQ=60)
                                 TGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGC                                                              >  SRR4302537.3311794/1‑94 (MQ=60)
                                  GGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCG                                                             >  SRR4302537.5691786/1‑94 (MQ=60)
                                    CGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGG                                                           >  SRR4302537.3765215/1‑94 (MQ=60)
                                     GAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGT                                                          <  SRR4302537.6045402/94‑1 (MQ=60)
                                     GAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGT                                                          <  SRR4302537.677436/94‑1 (MQ=60)
                                     GAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGT                                                          <  SRR4302537.4961742/94‑1 (MQ=60)
                                     GAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGT                                                          >  SRR4302537.926308/1‑94 (MQ=60)
                                      AAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTG                                                         >  SRR4302537.973712/1‑94 (MQ=60)
                                        AGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTT                                                       >  SRR4302537.3961208/1‑94 (MQ=60)
                                         GATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTA                                                      >  SRR4302537.6272073/1‑94 (MQ=60)
                                         GATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTA                                                      >  SRR4302537.6432715/1‑94 (MQ=60)
                                          ATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTAC                                                     <  SRR4302537.372776/94‑1 (MQ=60)
                                          ATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTAC                                                     <  SRR4302537.2667870/94‑1 (MQ=60)
                                          ATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTAC                                                     >  SRR4302537.3004296/1‑94 (MQ=60)
                                           TCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACC                                                    <  SRR4302537.3272707/94‑1 (MQ=60)
                                              CGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCG                                                 <  SRR4302537.1646478/94‑1 (MQ=60)
                                               GACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGG                                                >  SRR4302537.4765319/1‑94 (MQ=60)
                                                 CGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCCCGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCC                                              <  SRR4302537.2699185/94‑1 (MQ=60)
                                                  GAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCT                                             <  SRR4302537.4958221/94‑1 (MQ=60)
                                                  GAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCT                                             >  SRR4302537.3219684/1‑94 (MQ=60)
                                                  GAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCAGTGTCACGTGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCT                                             >  SRR4302537.4586540/1‑94 (MQ=60)
                                                   AGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTC                                            <  SRR4302537.4572645/94‑1 (MQ=60)
                                                     GCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGA                                          <  SRR4302537.126768/94‑1 (MQ=60)
                                                      CGGCTGAACTGGCGGCTTCCGGGGAGATCCCCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGAT                                         <  SRR4302537.2610254/94‑1 (MQ=60)
                                                      CGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGAT                                         <  SRR4302537.1670383/94‑1 (MQ=60)
                                                      CGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGAT                                         <  SRR4302537.3746065/94‑1 (MQ=60)
                                                       GGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATG                                        <  SRR4302537.4198009/94‑1 (MQ=60)
                                                         CTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTT                                      >  SRR4302537.221155/1‑94 (MQ=60)
                                                          TGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTT                                     >  SRR4302537.240218/1‑94 (MQ=60)
                                                            AACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTAT                                   <  SRR4302537.4173164/94‑1 (MQ=60)
                                                            AACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTAT                                   >  SRR4302537.5390975/1‑94 (MQ=60)
                                                            AACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGACCGACTGCGTGGGATCGATGGCGGGTGTTACGTGGGACTCGGTGTTTAT                                   >  SRR4302537.1453105/1‑94 (MQ=60)
                                                             ACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTAGCTCGGCCTCGATGTTTATT                                  >  SRR4302537.2038185/1‑94 (MQ=60)
                                                             ACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATG                                  <  SRR4302537.5030275/94‑1 (MQ=60)
                                                             ACTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATG                                  >  SRR4302537.3793809/1‑94 (MQ=60)
                                                              CTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGC                                 >  SRR4302537.4336522/1‑94 (MQ=60)
                                                              CTGGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGC                                 >  SRR4302537.5212777/1‑94 (MQ=60)
                                                                GGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACTAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCAC                               <  SRR4302537.6480182/94‑1 (MQ=60)
                                                                GGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCAC                               >  SRR4302537.2316807/1‑94 (MQ=60)
                                                                GGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCAC                               <  SRR4302537.5532881/94‑1 (MQ=60)
                                                                GGCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCAC                               >  SRR4302537.1728264/1‑94 (MQ=60)
                                                                 GCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACA                              <  SRR4302537.3594283/94‑1 (MQ=60)
                                                                 GCGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACA                              <  SRR4302537.3085157/94‑1 (MQ=60)
                                                                  CGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACAT                             <  SRR4302537.4600914/94‑1 (MQ=60)
                                                                  CGGCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACAT                             <  SRR4302537.1236388/94‑1 (MQ=60)
                                                                    GCTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCG                           <  SRR4302537.4405420/94‑1 (MQ=60)
                                                                     CTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGT                          >  SRR4302537.1914720/1‑94 (MQ=60)
                                                                     CTTCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGT                          >  SRR4302537.6478287/1‑94 (MQ=60)
                                                                       TCCGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAA                        <  SRR4302537.2088723/94‑1 (MQ=60)
                                                                         CGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAA                      >  SRR4302537.6060593/1‑94 (MQ=60)
                                                                         CGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAA                      >  SRR4302537.4071019/1‑94 (MQ=60)
                                                                         CGGGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAA                      >  SRR4302537.235782/1‑94 (MQ=60)
                                                                          GGGGAGATCACCTTCTCGCTGTGTCACAAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAA                     >  SRR4302537.248612/1‑94 (MQ=60)
                                                                           GGGAGATCTCCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAA                    <  SRR4302537.989332/94‑1 (MQ=60)
                                                                           GGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAA                    <  SRR4302537.1130185/94‑1 (MQ=60)
                                                                           GGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAA                    >  SRR4302537.2726079/1‑94 (MQ=60)
                                                                           GGGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAA                    <  SRR4302537.3021024/94‑1 (MQ=60)
                                                                            GGAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAAC                   <  SRR4302537.6401431/94‑1 (MQ=60)
                                                                             GAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACA                  <  SRR4302537.6445285/94‑1 (MQ=60)
                                                                             GAGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACA                  <  SRR4302537.5053463/94‑1 (MQ=60)
                                                                              AGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAA                 >  SRR4302537.29597/1‑94 (MQ=60)
                                                                              AGATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAA                 <  SRR4302537.562050/94‑1 (MQ=60)
                                                                               GATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAA                >  SRR4302537.2288618/1‑94 (MQ=60)
                                                                                ATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAA               >  SRR4302537.4140349/1‑94 (MQ=60)
                                                                                ATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAA               <  SRR4302537.1647491/94‑1 (MQ=60)
                                                                                ATCACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAA               <  SRR4302537.3357596/94‑1 (MQ=60)
                                                                                  CACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACC             <  SRR4302537.2530505/94‑1 (MQ=60)
                                                                                  CACCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACC             >  SRR4302537.1302099/1‑94 (MQ=60)
                                                                                   CCCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCT            <  SRR4302537.799503/94‑1 (MQ=60)
                                                                                    CCTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTA           <  SRR4302537.5017327/94‑1 (MQ=60)
                                                                                     CTTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTAC          <  SRR4302537.3353136/94‑1 (MQ=60)
                                                                                      TTCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACG         <  SRR4302537.5802026/94‑1 (MQ=60)
                                                                                       TCTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGG        <  SRR4302537.6079989/94‑1 (MQ=60)
                                                                                        CTCGCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGGC       >  SRR4302537.4401268/1‑94 (MQ=60)
                                                                                           GCTGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGGAAAC    >  SRR4302537.2510546/1‑94 (MQ=60)
                                                                                             TGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGGCAACAT  <  SRR4302537.3856874/94‑1 (MQ=60)
                                                                                             TGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGGCAACAT  <  SRR4302537.1288685/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCGCGGTCACCGGAGCGCTGGTGTTCGAAGGACTGGCGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAAACCTACGGCAACAT  >  NC_000913/337895‑338081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑