Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 346,725 A→G F315L (TTC→CTC)  prpR ← regulator for prp operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913346,7250AG94.7% 591.8 / 13.0 190F315L (TTC→CTC) prpRregulator for prp operon
Reads supporting (aligned to +/- strand):  ref base A (8/2);  new base G (129/51);  total (137/53)
Fisher's exact test for biased strand distribution p-value = 7.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.49e-01

Only 100 of 237 total aligned reads displayed.

CTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCG  >  NC_000913/346635‑346814
                                                                                          |                                                                                         
cttcCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCa                                                                                         <  2:510184/93‑1 (MQ=255)
 ttcCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAg                                                                                        <  2:5012703/93‑1 (MQ=255)
  tcCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGagcagc                                                                                       <  1:4767160/93‑1 (MQ=255)
    cAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGccc                                                                                     >  2:2639765/1‑93 (MQ=255)
    cAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGccc                                                                                     >  2:1314446/1‑93 (MQ=255)
    cAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGccc                                                                                     <  1:3104959/93‑1 (MQ=255)
         cccGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGgcgc                                                                                >  2:2198039/1‑93 (MQ=255)
          ccGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGcgcg                                                                               >  1:1225354/1‑93 (MQ=255)
           cGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGa                                                                              >  1:3453916/1‑93 (MQ=255)
            gcagcaGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAACAGCCCGGCGCGAc                                                                             >  2:1716666/1‑93 (MQ=255)
             cagcagCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGAcc                                                                            >  1:4698824/1‑93 (MQ=255)
               gcagcCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTc                                                                          >  2:3716794/1‑93 (MQ=255)
               gcagcCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTc                                                                          <  1:3029151/93‑1 (MQ=255)
                cagcCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTcc                                                                         <  2:923636/93‑1 (MQ=255)
                cagcCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTcc                                                                         <  2:4795218/93‑1 (MQ=255)
                  gcCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCcgc                                                                       >  1:1459004/1‑93 (MQ=255)
                  gcCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCcgc                                                                       >  1:4489666/1‑93 (MQ=255)
                  gcCGGGTCTACAGAGGTAGCGGTATTTCGCCAATCTCATCAAGAAACAGCGTACCGCCGTCGGCAATTTCGAGCAGCCTGTCTCGACCTCcgc                                                                       >  2:3806422/1‑93 (MQ=25)
                   ccGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCcgcg                                                                      <  1:214190/93‑1 (MQ=255)
                    cGGGTCTGCAAAGGTAGCGGCATTTCGCCAATGTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGGCCTCCGCGt                                                                     <  1:6045807/93‑1 (MQ=255)
                    cGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGt                                                                     >  1:6108584/1‑93 (MQ=255)
                    cGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGt                                                                     >  2:1958963/1‑93 (MQ=255)
                     gggTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTc                                                                    <  1:1152911/93‑1 (MQ=255)
                     gggTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTc                                                                    <  2:67330/93‑1 (MQ=255)
                         cTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGa                                                                <  2:4901280/93‑1 (MQ=255)
                         cTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGa                                                                <  1:3737741/93‑1 (MQ=255)
                          tGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAg                                                               <  1:1557013/93‑1 (MQ=255)
                          tGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAg                                                               >  1:3580901/1‑93 (MQ=255)
                            cAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGcc                                                             >  1:3596236/1‑93 (MQ=255)
                            cAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGcc                                                             >  2:4026899/1‑93 (MQ=255)
                            cAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGcc                                                             >  1:5201823/1‑93 (MQ=255)
                             aaaGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCg                                                            >  2:6111771/1‑93 (MQ=255)
                             aaaGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCg                                                            >  1:1012988/1‑93 (MQ=255)
                              aaGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCgg                                                           >  2:223275/1‑93 (MQ=255)
                              aaGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGACGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCgg                                                           >  2:2107627/1‑93 (MQ=255)
                                    gCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACg                                                     >  2:3873065/1‑93 (MQ=255)
                                     cGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTACACGc                                                    >  2:1518245/1‑93 (MQ=255)
                                      ggCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTcgcg                                                                 >  1:93986/1‑79 (MQ=255)
                                      ggCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTcgcg                                                                 >  1:1993802/1‑79 (MQ=255)
                                      ggCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGcc                                                   >  1:2027702/1‑93 (MQ=255)
                                      ggCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGcc                                                   >  1:6193364/1‑93 (MQ=255)
                                      ggCATTTCGCCAATCTCAACCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGcc                                                   >  1:5923056/1‑93 (MQ=255)
                                       gCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGCAAACGccc                                                  >  1:3950608/1‑93 (MQ=255)
                                         aTTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCt                                                >  1:184115/1‑93 (MQ=255)
                                         aTTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCt                                                >  2:2331873/1‑93 (MQ=255)
                                         aTTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCt                                                >  1:2377769/1‑93 (MQ=255)
                                          tttCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCGCCtt                                               >  1:2249168/1‑93 (MQ=255)
                                          tttCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCtt                                               >  1:4570589/1‑93 (MQ=255)
                                          tttCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCtt                                               >  2:2927708/1‑93 (MQ=255)
                                            tCGCCAATCTCATCCAGAAACAGCGTCCCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGCCCTCCGCGTCGCGAGCCGGTAAACGCCCCTTcc                                             <  1:2837140/93‑1 (MQ=255)
                                            tCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTcc                                             >  2:5269561/1‑93 (MQ=255)
                                             cGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCt                                            >  2:4701937/1‑93 (MQ=255)
                                              gCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTc                                           >  2:5678347/1‑93 (MQ=255)
                                               ccAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCa                                          >  2:6179225/1‑93 (MQ=255)
                                                cAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGCCCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCAt                                         <  1:2063828/93‑1 (MQ=255)
                                                 aaTCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATa                                        >  1:2081201/1‑93 (MQ=255)
                                                  aTCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAg                                       >  2:114375/1‑93 (MQ=255)
                                                  aTCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAg                                       >  1:3172113/1‑93 (MQ=255)
                                                   tctcATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGc                                      >  1:5436587/1‑93 (MQ=255)
                                                   tctcATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGc                                      >  1:4302039/1‑93 (MQ=255)
                                                    ctcATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGcc                                     >  2:4516264/1‑93 (MQ=255)
                                                     tcATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCa                                    >  2:3200620/1‑93 (MQ=255)
                                                       aTCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCaaa                                  >  2:3032277/1‑93 (MQ=255)
                                                        tCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAAc                                 >  2:829559/1‑93 (MQ=255)
                                                        tCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCCTCCTCATAGCCAAAc                                 >  1:2319348/1‑93 (MQ=255)
                                                        tCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGACTCGGTAAACGCCCCTTCCTCATAGCCaacc                                 >  1:4152813/1‑91 (MQ=255)
                                                         ccAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACa                                >  1:6008588/1‑93 (MQ=255)
                                                            aaaaCAGCGTCCCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGCCCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGtt                             <  2:2985198/92‑1 (MQ=255)
                                                             aaaCAGCTTCCCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCCCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTc                            <  2:390168/93‑1 (MQ=255)
                                                             aaaCAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTc                            >  1:6412203/1‑93 (MQ=255)
                                                              aaCAGCGTCCCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCt                           <  1:1761888/93‑1 (MQ=255)
                                                               aCAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTg                          >  2:786894/1‑93 (MQ=255)
                                                                ccgCGTCCCGCGGGGGCCAATTTCGAGCAGCCCGGCGCCCCCCCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGc                         <  2:4414213/91‑1 (MQ=255)
                                                                cAGCGTCCCGCCGTGGGCAATTTCGAACAGCCCGGCGCTCCCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGc                         <  1:1125931/93‑1 (MQ=255)
                                                                  gCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCtt                       >  1:406173/1‑93 (MQ=255)
                                                                   cGTCCCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTc                      <  2:419489/93‑1 (MQ=255)
                                                                   cGTCCCGCCGTGGGCAATTCCGAGCAGCCCGGCGCGCCCTCCGCGTCGGGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTc                      <  2:5067118/93‑1 (MQ=255)
                                                                   cGTCCCGCCGGGGGCATTTCCGAGCGCCCCGGCGCGCCCCCCGCTTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTc                      <  1:5878576/93‑1 (MQ=255)
                                                                    gTACCGCCGTGGGCAATTTCGAGCAGCCGGGCGCGACCTCCGCGTCGGGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTcc                     <  2:1992954/93‑1 (MQ=255)
                                                                     tACCGCCGTGGGCAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCca                    >  2:3388834/1‑93 (MQ=255)
                                                                     ccccgccgTGGCCAATTTCGAGCAGCCCGGCGCGCCCCCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCca                    <  2:140364/91‑1 (MQ=255)
                                                                       ccgccgTGGACAATTCCGACCAGCCCGGCCCCCCCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCcagc                  <  1:2683311/93‑1 (MQ=255)
                                                                        cgccgTGGGCAATTCCGAGCAGCCGGGCGCGCCCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCcagca                 <  2:2004498/93‑1 (MQ=255)
                                                                        cgccgGGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCcagca                 <  1:4821912/93‑1 (MQ=255)
                                                                         gccgTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCcagcag                >  1:5667987/1‑93 (MQ=255)
                                                                          cggTGGGAAATTCCGAGCAGCCGGGCGCGCCCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCagcagc               <  1:4559030/91‑1 (MQ=255)
                                                                          ccgTGGGCATTTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCagcagc               <  1:5271964/93‑1 (MQ=255)
                                                                          ccgTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCagcagc               >  1:4972303/1‑93 (MQ=255)
                                                                          ccgTGGCGAATTTCGAGCAGCCCGGCGCGACCTCCGCGGCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCagcagc               <  2:6417316/93‑1 (MQ=255)
                                                                          ccgGGGGAATTTTCGAGCAGCCGGGCGCGCCCCCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCagcagc               <  1:3898243/93‑1 (MQ=255)
                                                                            gTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGa             >  1:5324810/1‑93 (MQ=255)
                                                                            gTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGa             >  2:6076726/1‑93 (MQ=255)
                                                                            gTGGGCAATTGCGAGCAGCCCGGCGCGCCCCCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGa             <  1:834694/93‑1 (MQ=255)
                                                                             tGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGAt            >  2:1611011/1‑93 (MQ=255)
                                                                             tGGGCAATGTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGAt            <  2:3708495/93‑1 (MQ=255)
                                                                                gaattttCGAGCAGCCCGGCCCGCCCCCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCg         <  1:4411005/89‑1 (MQ=255)
                                                                                ccattttCGAGCAGCCGGGCGCGACCTCCGCGCCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCATACAGTTCTGCTTCCAGCAGCGATTCg         <  2:469174/89‑1 (MQ=255)
                                                                                  aaTTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGc       >  2:5478220/1‑93 (MQ=255)
                                                                                  aaTTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGc       >  2:1565823/1‑93 (MQ=255)
                                                                                    tttCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCaa     >  1:1708352/1‑93 (MQ=255)
                                                                                          |                                                                                         
CTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCG  >  NC_000913/346635‑346814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCC  >  NC_000913/346632‑346818
                                                                                             |                                                                                             
TTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAG                                                                                               <  SRR4302537.5562008/94‑1 (MQ=60)
TTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAG                                                                                               <  SRR4302537.4400330/94‑1 (MQ=60)
 TTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGC                                                                                              <  SRR4302537.698999/94‑1 (MQ=60)
 TTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGC                                                                                              <  SRR4302537.6377942/94‑1 (MQ=60)
   CTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGGATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAG                                                                                            <  SRR4302537.4187253/94‑1 (MQ=60)
     TCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCC                                                                                          <  SRR4302537.4800081/94‑1 (MQ=60)
      CCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCC                                                                                         <  SRR4302537.3082798/94‑1 (MQ=60)
      CCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAACAGCCC                                                                                         >  SRR4302537.5265044/1‑94 (MQ=60)
       CAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCG                                                                                        <  SRR4302537.3126647/94‑1 (MQ=60)
        AGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGG                                                                                       >  SRR4302537.4637855/1‑94 (MQ=60)
        AGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGG                                                                                       >  SRR4302537.5692605/1‑94 (MQ=60)
            CCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCG                                                                                   >  SRR4302537.1233869/1‑94 (MQ=60)
             CCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGA                                                                                  >  SRR4302537.3478341/1‑94 (MQ=60)
               GCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACC                                                                                >  SRR4302537.4731334/1‑94 (MQ=60)
                CAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCT                                                                               >  SRR4302537.860862/1‑94 (MQ=60)
                  GCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCC                                                                             <  SRR4302537.1650555/94‑1 (MQ=60)
                  GCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCC                                                                             <  SRR4302537.3050270/94‑1 (MQ=60)
                    AGCCGGGTCTGCAAAGGTAGCGGTATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGC                                                                           >  SRR4302537.3164190/1‑94 (MQ=60)
                    AGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGC                                                                           >  SRR4302537.1469159/1‑94 (MQ=60)
                    AGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGC                                                                           >  SRR4302537.4520724/1‑94 (MQ=60)
                      CCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGT                                                                         >  SRR4302537.212449/1‑94 (MQ=60)
                      CCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGT                                                                         <  SRR4302537.215716/94‑1 (MQ=60)
                      CCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGT                                                                         >  SRR4302537.6150560/1‑94 (MQ=60)
                       CGGGTCTGCAAAGGTAGCGGCATTTCGCCAATGTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGGCCTCCGCGTC                                                                        <  SRR4302537.6087328/94‑1 (MQ=60)
                       CGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTC                                                                        <  SRR4302537.4960155/94‑1 (MQ=60)
                        GGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCG                                                                       >  SRR4302537.2073576/1‑94 (MQ=60)
                        GGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCG                                                                       >  SRR4302537.3926380/1‑94 (MQ=60)
                        GGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCG                                                                       <  SRR4302537.1160932/94‑1 (MQ=60)
                         GGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGC                                                                      >  SRR4302537.3337822/1‑94 (MQ=60)
                          GTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCG                                                                     >  SRR4302537.5730821/1‑94 (MQ=60)
                            CTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAG                                                                   <  SRR4302537.583762/94‑1 (MQ=60)
                            CTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAG                                                                   >  SRR4302537.3606144/1‑94 (MQ=60)
                            CTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAG                                                                   >  SRR4302537.1696917/1‑94 (MQ=60)
                            CTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAG                                                                   <  SRR4302537.3763987/94‑1 (MQ=60)
                             TGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGC                                                                  >  SRR4302537.3021266/1‑94 (MQ=60)
                             TGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGC                                                                  <  SRR4302537.1567883/94‑1 (MQ=60)
                              GCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCC                                                                 >  SRR4302537.3621588/1‑94 (MQ=60)
                              GCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCC                                                                 >  SRR4302537.5237606/1‑94 (MQ=60)
                              GCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCC                                                                 >  SRR4302537.4995171/1‑94 (MQ=60)
                               CAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCG                                                                >  SRR4302537.1019970/1‑94 (MQ=60)
                               CAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCG                                                                <  SRR4302537.1378392/94‑1 (MQ=60)
                                AAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGG                                                               >  SRR4302537.1146150/1‑94 (MQ=60)
                                 AAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGCCCTCCGCGTCGCGAGCCGGT                                                              <  SRR4302537.6102139/94‑1 (MQ=60)
                                 AAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGT                                                              >  SRR4302537.5958621/1‑94 (MQ=60)
                                    GTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAC                                                           >  SRR4302537.1798392/1‑94 (MQ=60)
                                    GTAGCGGCATTCCGCCAATCTCATCCAGAAACAGCGTCCCGCCGTGGGCAATTCCGAGCAGCCCGGCGCGCCCTCCGCGTCGCGAGCCGGTAAA                                                           <  SRR4302537.1246645/94‑1 (MQ=60)
                                      AGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTCCCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACG                                                         <  SRR4302537.3799994/94‑1 (MQ=60)
                                      AGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACG                                                         >  SRR4302537.4774810/1‑94 (MQ=60)
                                       GCGGCATTTCGCAAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGC                                                        <  SRR4302537.3699507/94‑1 (MQ=60)
                                        CGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAacggaatagatcg                                                       >  SRR4302537.2007666/1‑81 (MQ=60)
                                        CGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAacggaatagatcg                                                       >  SRR4302537.94671/1‑81 (MQ=60)
                                        CGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCC                                                       >  SRR4302537.539204/1‑94 (MQ=60)
                                        CGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCC                                                       >  SRR4302537.2041840/1‑94 (MQ=60)
                                        CGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCC                                                       >  SRR4302537.6236038/1‑94 (MQ=60)
                                        CGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCC                                                       >  SRR4302537.3632090/1‑94 (MQ=60)
                                        CGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCC                                                       <  SRR4302537.2892945/94‑1 (MQ=60)
                                        CGGCATTTCGCCAATCTCAACCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCC                                                       >  SRR4302537.5963696/1‑94 (MQ=60)
                                         GGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCC                                                      >  SRR4302537.3978239/1‑94 (MQ=60)
                                         GGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGCAAACGCCC                                                      >  SRR4302537.3978238/1‑94 (MQ=60)
                                           CATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCT                                                    >  SRR4302537.5325055/1‑94 (MQ=60)
                                           CATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCT                                                    >  SRR4302537.2171575/1‑94 (MQ=60)
                                           CATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCT                                                    >  SRR4302537.2394524/1‑94 (MQ=60)
                                           CATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCT                                                    >  SRR4302537.185427/1‑94 (MQ=60)
                                            ATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCGCCTT                                                   >  SRR4302537.2265042/1‑94 (MQ=60)
                                            ATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTT                                                   >  SRR4302537.4602223/1‑94 (MQ=60)
                                            ATTTCGCCAACCTCATCCAGAAACAGCGTCCCGCCGTGGGCAATTGCGAGCAGCCCGGCGCGCCCTCGGCGCCGCGAGCCGGTAAACGCCCCTT                                                   <  SRR4302537.2891681/94‑1 (MQ=60)
                                            ATTTCGAAAACCTCATCCAGAAACAGCGTCCCGCCGGGGACATTTTGGAGACGCCCGGCGCGCCCCCCGCGTCGCGAGCCGGTAAACGCCCCTT                                                   <  SRR4302537.3495162/94‑1 (MQ=21)
                                             TTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGCCCTCCGCGTCGCGAGCCGGTAAACGCCCCTTC                                                  <  SRR4302537.1768318/94‑1 (MQ=60)
                                              TTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCC                                                 >  SRR4302537.1054535/1‑94 (MQ=60)
                                              TTCGCAAATCACAGCCAGAACCAGCCTACCGCCGTGGGCATTTCCGAGCAGCCCGGCGCCACCCCCCCGTCGGGAGCCGGTAAACGCCCCTTCC                                                 <  SRR4302537.2424048/94‑1 (MQ=25)
                                              TTCGCAAACCTCATCCAGAAACAGCGTCCCGCCGTGGGCAATTTCGAACAGCCCGGCGCGCCCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCC                                                 <  SRR4302537.6217948/94‑1 (MQ=60)
                                               TCGCCAATCTCATCCAGAAACAGCGTCCCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGCCCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCT                                                <  SRR4302537.2856989/94‑1 (MQ=60)
                                               TCGCCAATCTCATCCAGAAACAGCGTCCCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCT                                                <  SRR4302537.585379/94‑1 (MQ=60)
                                                CGCCAATCTCATCCAGAAACAGCGTCCCGCGGTGGGCAATTTCGAGCAGCCCGGCGCGCCCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTC                                               <  SRR4302537.1078094/94‑1 (MQ=60)
                                                CGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTC                                               >  SRR4302537.359528/1‑94 (MQ=60)
                                                CGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTC                                               <  SRR4302537.2079217/94‑1 (MQ=60)
                                                CGCCAACCTCACCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCCCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTC                                               <  SRR4302537.2442252/94‑1 (MQ=60)
                                                 GCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCA                                              >  SRR4302537.639300/1‑94 (MQ=60)
                                                   CAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGCCCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATA                                            <  SRR4302537.2078319/94‑1 (MQ=60)
                                                   CAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATA                                            >  SRR4302537.5021762/1‑94 (MQ=60)
                                                   CAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATA                                            >  SRR4302537.2095860/1‑94 (MQ=60)
                                                    AATCTCATCCAGAAACAGCGTCCCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGCCCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAG                                           <  SRR4302537.2639579/94‑1 (MQ=60)
                                                    AATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAG                                           >  SRR4302537.3194400/1‑94 (MQ=60)
                                                    AATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAC                                           >  SRR4302537.4123933/1‑94 (MQ=60)
                                                     ATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGC                                          >  SRR4302537.4331936/1‑94 (MQ=60)
                                                     ATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGC                                          >  SRR4302537.5473670/1‑94 (MQ=60)
                                                     ATCGCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGTGACCTCCGCGTCGCGTGCCGGTAAACGCCCCGTCCTCATAGC                                          >  SRR4302537.6517363/1‑94 (MQ=60)
                                                      TCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCC                                         >  SRR4302537.2033622/1‑94 (MQ=60)
                                                        TCATCCAGAAACGGCGTCCCGCCGTGGGCAATTTCGAGCAGCCGGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAA                                       <  SRR4302537.1193695/94‑1 (MQ=60)
                                                          ATCCAGAAACAGCGTACCGCGGTGGGAAATTCCGAGCAGCCGGGCGCGCCCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAAC                                     <  SRR4302537.6194733/94‑1 (MQ=60)
                                                          ATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAAC                                     >  SRR4302537.4346435/1‑94 (MQ=60)
                                                          ATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCCTCCTCATAGCCAAAC                                     >  SRR4302537.2335726/1‑94 (MQ=60)
                                                          ATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGACTCGGTAAACGCCCCTTCCTCATAGCCAACC                                     >  SRR4302537.4181810/1‑94 (MQ=60)
                                                           ccaAGAAACAGCGTCCCGCCGTGGGCAATTCCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACA                                    <  SRR4302537.233241/91‑1 (MQ=60)
                                                           ccaAGAAACAGCGTACCGCGGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACA                                    <  SRR4302537.1990001/91‑1 (MQ=60)
                                                           TCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACA                                    >  SRR4302537.6049873/1‑94 (MQ=60)
                                                              AGAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTT                                 >  SRR4302537.275034/1‑94 (MQ=60)
                                                               GAAACAGCGTCCCGCCAGGGGCAATTTCGAGCAGCCCGGCGCGCCCCCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTC                                <  SRR4302537.4041887/94‑1 (MQ=60)
                                                               GAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTC                                <  SRR4302537.5327780/94‑1 (MQ=60)
                                                               GAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTC                                >  SRR4302537.2057397/1‑94 (MQ=60)
                                                               GAAACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTC                                >  SRR4302537.6455823/1‑94 (MQ=60)
                                                                 AACAGCGTCCCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTG                              <  SRR4302537.1774165/94‑1 (MQ=60)
                                                                 AACAGCGTCCCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGCCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTG                              <  SRR4302537.4580631/94‑1 (MQ=60)
                                                                 AACAGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTG                              >  SRR4302537.849621/1‑94 (MQ=60)
                                                                 AACAGCGTACCCCCGTGGGCAATTTCGAGCACCCCGGCGCGACCCCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTG                              <  SRR4302537.432984/94‑1 (MQ=60)
                                                                  ACAGCGTCCCGCGGTGGGCAATTTCGAGCAGCCCGGCGCGCCCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGC                             <  SRR4302537.198903/94‑1 (MQ=60)
                                                                   CAGCGTCCCGCCGTGGGCAATTTCGAACAGCCCGGCGCTCCCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCT                            <  SRR4302537.1133737/94‑1 (MQ=60)
                                                                   CAGCGTCCCCCCGTGGGCAATTTCGAGCAGCCCGGCGCGCCCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCT                            <  SRR4302537.4807716/94‑1 (MQ=60)
                                                                    AGCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTT                           >  SRR4302537.408988/1‑94 (MQ=60)
                                                                     GCGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTC                          >  SRR4302537.4746294/1‑94 (MQ=60)
                                                                      CGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGCCCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCC                         <  SRR4302537.6152253/94‑1 (MQ=60)
                                                                      CGTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCC                         >  SRR4302537.3613112/1‑94 (MQ=60)
                                                                       GTACCGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCA                        >  SRR4302537.6038782/1‑94 (MQ=60)
                                                                        tccccccggGGGCAATTCCGAGCAGCCGGGCGCGCCCCCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAG                       <  SRR4302537.5838932/85‑1 (MQ=60)
                                                                          CCGCCGTGGACAATTCCGACCAGCCCGGCCCCCCCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCA                     <  SRR4302537.2702043/94‑1 (MQ=60)
                                                                           CGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAG                    >  SRR4302537.5706768/1‑94 (MQ=60)
                                                                           CGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAG                    >  SRR4302537.1181243/1‑94 (MQ=60)
                                                                           CGCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAG                    >  SRR4302537.5996466/1‑94 (MQ=60)
                                                                           CGCCGGGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAG                    <  SRR4302537.4855219/94‑1 (MQ=60)
                                                                            GCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGC                   >  SRR4302537.2210671/1‑94 (MQ=60)
                                                                            GCCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGC                   >  SRR4302537.5006575/1‑94 (MQ=60)
                                                                             cgggGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCG                  <  SRR4302537.4485754/90‑1 (MQ=60)
                                                                             ccgggggaatTTTCGAGCAGCCGGGCGCGCCCCCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCG                  <  SRR4302537.3925495/84‑1 (MQ=60)
                                                                             CGGTGGGAAATTCCGAGCAGCCGGGCGCGCCCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCG                  <  SRR4302537.4590588/94‑1 (MQ=60)
                                                                             CCGTGGGCATTTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCG                  <  SRR4302537.5308246/94‑1 (MQ=60)
                                                                             CCGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCG                  <  SRR4302537.3235458/94‑1 (MQ=60)
                                                                              CGTGGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGA                 >  SRR4302537.5361349/1‑94 (MQ=60)
                                                                               GTGGGCAATTGCGAGCAGCCCGGCGCGCCCCCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGAT                <  SRR4302537.840406/94‑1 (MQ=60)
                                                                                 GGGCAATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTC              >  SRR4302537.6057260/1‑94 (MQ=60)
                                                                                 GGGCAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTC              >  SRR4302537.2365288/1‑94 (MQ=60)
                                                                                 GGGCAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTC              >  SRR4302537.2597849/1‑94 (MQ=60)
                                                                                 GGGAAATTTCGAGCACCCCGGCGCGCCCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTC              <  SRR4302537.1522790/94‑1 (MQ=60)
                                                                                  GGCAATTTCGAGCAGCCCGGCGCGACCTCTGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCG             >  SRR4302537.4557055/1‑94 (MQ=60)
                                                                                  GGCAATTCCGACCAGCCCGGCGCGACCTCCGCTTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCG             <  SRR4302537.2506735/94‑1 (MQ=60)
                                                                                   gaatTTTCGAGCAGCCCGGCCCGCCCCCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGG            <  SRR4302537.4441441/90‑1 (MQ=60)
                                                                                     AATTTCGAGCAGCCGGGCGCGCCCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCA          <  SRR4302537.4602164/94‑1 (MQ=60)
                                                                                      ATTTCGAGCAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAA         >  SRR4302537.1720292/1‑94 (MQ=60)
                                                                                        TTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATC       >  SRR4302537.1177156/1‑94 (MQ=60)
                                                                                          CGAGCAGCCCGGCGCCCCCCCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGC     <  SRR4302537.988748/94‑1 (MQ=60)
                                                                                             GCAGCCGGGCGCGACCTCGGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCC  <  SRR4302537.6436546/94‑1 (MQ=60)
                                                                                             |                                                                                             
TTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAACAGCCCGGCGCGACCTCCGCGTCGCGAGCCGGTAAACGCCCCTTCCTCATAGCCAAACAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCC  >  NC_000913/346632‑346818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑