Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	375,923	A→G	intergenic (+29/‑73)	mhpT → / → yaiL	putative transport protein/nucleoprotein/polynucleotide‑associated enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	375,923	0	A	G	99.5%	745.9 / NA	219	intergenic (+29/‑73)	mhpT/yaiL	putative transport protein/nucleoprotein/polynucleotide‑associated enzyme
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (126/92); minor base C (0/1); total (126/93)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 227 total aligned reads displayed.

GGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCTT  >  NC_000913/375833‑376012

 gTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCtt                                                                                          <  1:3847969/93‑1 (MQ=255)

  ttGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTc                                                                                         >  1:1386538/1‑93 (MQ=255)

  ttGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTc                                                                                         >  1:2371480/1‑93 (MQ=255)

    ggCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCAc                                                                                       >  1:4709099/1‑93 (MQ=255)

     gCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCAcc                                                                                      >  2:2970019/1‑93 (MQ=255)

     gCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCAcc                                                                                      >  1:2452768/1‑93 (MQ=255)

     gCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCCGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCAcc                                                                                      >  1:3926372/1‑93 (MQ=255)

      cGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCAccc                                                                                     <  2:2946249/93‑1 (MQ=255)

      cGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCAccc                                                                                     >  1:2712700/1‑93 (MQ=255)

       ggTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCAcccc                                                                                    >  1:3313391/1‑93 (MQ=255)

          gtgtATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAAt                                                                                 <  1:2036303/90‑1 (MQ=255)

          gtTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAAt                                                                                 >  1:3884700/1‑93 (MQ=255)

          gtTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAAt                                                                                 <  2:1512158/93‑1 (MQ=255)

           tttATTTTGATGAGCCGGAGATCACGAATACAGCCGTTCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATa                                                                                <  2:5356468/93‑1 (MQ=255)

           tttATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATa                                                                                >  1:1694201/1‑93 (MQ=255)

           tttATTTTGATGAGCCGGAAATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATa                                                                                >  2:6331849/1‑93 (MQ=255)

             tATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATa‑ca                                                                             >  1:5087303/1‑93 (MQ=255)

              aTTTTGATGAGCCGGAGATCACGAATGCAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCGCCCCAATa‑cac                                                                            <  2:4873628/93‑1 (MQ=255)

              aTTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATa‑cac                                                                            <  1:1143447/93‑1 (MQ=255)

               ttttGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CAcc                                                                           >  2:1481754/1‑93 (MQ=255)

                tttGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCt                                                                          >  2:1682226/1‑93 (MQ=255)

                tttGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCt                                                                          <  2:3723589/93‑1 (MQ=255)

                  tgatgaGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTAc                                                                        <  1:1014522/93‑1 (MQ=255)

                  tgatgaGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTAc                                                                        <  1:4777277/93‑1 (MQ=255)

                  tgatgaGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTAc                                                                        <  2:6054715/93‑1 (MQ=255)

                    atgaGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACgg                                                                      >  2:3013697/1‑93 (MQ=255)

                     tgaGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGc                                                                     >  2:4038509/1‑93 (MQ=255)

                        gCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGtt                                                                  >  1:4974378/1‑93 (MQ=255)

                        gCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGtt                                                                  <  2:6157912/93‑1 (MQ=255)

                           ggAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTAc                                                               <  2:1391884/93‑1 (MQ=255)

                           ggAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAAGGGCTTCACCCCAATA‑CACCTACGGCGTTTAc                                                               >  1:2757612/1‑93 (MQ=255)

                            gagaTCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACa                                                              >  1:2393513/1‑93 (MQ=255)

                             agaTCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAg                                                             <  2:4308780/93‑1 (MQ=255)

                                tCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGta                                                          >  2:3467222/1‑93 (MQ=255)

                                tCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGta                                                          <  1:2816495/93‑1 (MQ=255)

                                tCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGta                                                          <  2:2277332/93‑1 (MQ=255)

                                tCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGta                                                          <  2:5952835/93‑1 (MQ=255)

                                   cGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TAc                                                      <  2:3788250/93‑1 (MQ=255)

                                   cGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TAc                                                      >  1:3725666/1‑93 (MQ=255)

                                    gAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACt                                                     <  1:6058169/93‑1 (MQ=255)

                                     aaTACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTc                                                    >  1:1828176/1‑93 (MQ=255)

                                      aTACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCg                                                   >  1:712818/1‑93 (MQ=255)

                                      aTACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCg                                                   >  2:5858272/1‑93 (MQ=255)

                                      aTACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGAGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCg                                                   >  1:4323067/1‑93 (MQ=255)

                                       tACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGc                                                  <  1:4769205/93‑1 (MQ=255)

                                        aCAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCt                                                 >  2:5564222/1‑93 (MQ=255)

                                        aCAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCt                                                 >  2:441776/1‑93 (MQ=255)

                                        aCAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCt                                                 >  2:2786678/1‑93 (MQ=255)

                                        aCAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCt                                                 >  2:1258411/1‑93 (MQ=255)

                                        aCAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCt                                                 <  1:1969080/93‑1 (MQ=255)

                                         cAGCCGTGCTCCGATGCCTGACGTGCCTTATTAGGCAGGGGCTAAACGGGC‑TCACCCCAATC‑CACCTGCGGCGTTTACAGGTA‑TACTCGCTaa                                               <  1:4962219/93‑1 (MQ=255)

                                         cAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTa                                                <  2:518958/93‑1 (MQ=255)

                                         cAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTa                                                <  1:6315721/93‑1 (MQ=255)

                                           gCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACATACGGCGTTTACAGGTA‑TACTCGCTaaa                                              >  1:4396601/1‑93 (MQ=255)

                                             cGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTaaaaa                                            >  1:122761/1‑93 (MQ=255)

                                                   ccGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTc                                      <  2:4928027/93‑1 (MQ=255)

                                                      aTGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGc                                   <  1:3980653/93‑1 (MQ=255)

                                                      aTGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGc                                   <  2:3000900/93‑1 (MQ=255)

                                                       tGCCTGACGTCCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCg                                  <  2:2428292/93‑1 (MQ=255)

                                                        gCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCgg                                 >  2:316309/1‑93 (MQ=255)

                                                        gCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCgg                                 <  2:735811/93‑1 (MQ=255)

                                                        gCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCgg                                 >  2:2651933/1‑93 (MQ=255)

                                                         ccTGACGTGCCTTATTAGGCAGGGGCTAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCggg                                <  1:5126970/93‑1 (MQ=255)

                                                         ccTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCggg                                <  1:2886385/93‑1 (MQ=255)

                                                         ccTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCggg                                >  2:3126608/1‑93 (MQ=255)

                                                          cTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGt                               >  1:2197936/1‑93 (MQ=255)

                                                           tGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGtt                              <  1:5627817/93‑1 (MQ=255)

                                                            gACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCAACCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGttt                             >  2:2161678/1‑93 (MQ=255)

                                                             aCGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTg                            <  2:4655418/93‑1 (MQ=255)

                                                             aCGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTg                            >  1:1351457/1‑93 (MQ=255)

                                                             aCGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTg                            <  2:1142963/93‑1 (MQ=255)

                                                               gTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGa                          <  2:5711754/93‑1 (MQ=255)

                                                                tGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTC‑TACTCGCTAAAAATTATTCAGCGGGTTTGGaa                         >  1:5217897/1‑93 (MQ=255)

                                                                tGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGaa                         >  1:1571678/1‑93 (MQ=255)

                                                                 gCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGaaa                        >  1:5713857/1‑93 (MQ=255)

                                                                  ccTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGAAAc                       >  2:2323646/1‑93 (MQ=255)

                                                                    ttattaGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGAAACaa                     <  1:1624036/93‑1 (MQ=255)

                                                                     tattaGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGAAACaaa                    <  1:3563569/93‑1 (MQ=255)

                                                                     tattaGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGAA‑TACTCGCTAAAAATTATGCAGCGGGTTTAGCAACaaa                    >  2:2383008/1‑93 (MQ=255)

                                                                      attaGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAg                   <  2:791553/93‑1 (MQ=255)

                                                                       ttaGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGa                  <  1:2940262/93‑1 (MQ=255)

                                                                       ttaGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGa                  >  1:6367677/1‑93 (MQ=255)

                                                                       ttaGGCAGGGGCGAAAGGAACTTCACCCCAATA‑CACCTACGGCGTTTACAGATA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGa                  <  1:3459015/93‑1 (MQ=255)

                                                                        taGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGAt                 >  2:4892477/1‑93 (MQ=255)

                                                                        taGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGAt                 >  2:1969059/1‑93 (MQ=255)

                                                                         aGGCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATg                <  2:3211805/93‑1 (MQ=255)

                                                                          ggCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATgg               <  1:3273648/93‑1 (MQ=255)

                                                                          ggCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATgg               >  1:6163178/1‑93 (MQ=255)

                                                                           gCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGc              <  1:4481732/93‑1 (MQ=255)

                                                                           gCAGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGc              >  1:556520/1‑93 (MQ=255)

                                                                             aGGGGCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCaa            >  2:3554699/1‑93 (MQ=255)

                                                                              ggggCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCaaa           >  2:4713948/1‑93 (MQ=255)

                                                                              ggggCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCaaa           <  1:6460303/93‑1 (MQ=255)

                                                                               gggCGAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGCTTGGAAACAAAGATGGCaaaa          >  2:3350352/1‑93 (MQ=255)

                                                                                tgCGAAAGGGGGTTTCCCCCCATACCACCTCCGGC‑TTTTCCGGTATTACTC‑CTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAAc         <  1:747091/92‑1 (MQ=255)

                                                                                   gAAAGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAActta      >  2:4502765/1‑93 (MQ=255)

                                                                                    aaaGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAActtac     >  1:3061163/1‑93 (MQ=255)

                                                                                     aaGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAActtacc    >  2:2608520/1‑93 (MQ=255)

                                                                                     aaGGGGCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAActtacc    >  2:1188937/1‑93 (MQ=255)

                                                                                       ggggCTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAActtacctt  >  1:5804888/1‑93 (MQ=255)

GGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATA‑CACCTACGGCGTTTACAGGTA‑TACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCTT  >  NC_000913/375833‑376012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCTTACAA  >  NC_000913/375830‑376016

CTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGG                                                                                               >  SRR4302537.2637562/1‑94 (MQ=60)

 TGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGC                                                                                              <  SRR4302537.4193465/94‑1 (MQ=60)

  GGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCT                                                                                             >  SRR4302537.5669384/1‑94 (MQ=60)

    GTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTC                                                                                           <  SRR4302537.3874911/94‑1 (MQ=60)

    GTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTC                                                                                           >  SRR4302537.1396206/1‑94 (MQ=60)

    GTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTC                                                                                           >  SRR4302537.2388191/1‑94 (MQ=60)

      TGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCAC                                                                                         >  SRR4302537.4741677/1‑94 (MQ=60)

       GGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACC                                                                                        >  SRR4302537.203154/1‑94 (MQ=60)

       GGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACC                                                                                        >  SRR4302537.2470025/1‑94 (MQ=60)

       GGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCCGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACC                                                                                        >  SRR4302537.3953804/1‑94 (MQ=60)

        GCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCC                                                                                       >  SRR4302537.2731644/1‑94 (MQ=60)

         CGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCC                                                                                      >  SRR4302537.3336830/1‑94 (MQ=60)

         CGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCC                                                                                      >  SRR4302537.3957867/1‑94 (MQ=60)

           GTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCTGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCCTCACCCCAA                                                                                    >  SRR4302537.4306656/1‑94 (MQ=60)

            TGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAAT                                                                                   >  SRR4302537.3911868/1‑94 (MQ=60)

             GTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA                                                                                  >  SRR4302537.2187140/1‑94 (MQ=60)

             GTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA                                                                                  >  SRR4302537.1706057/1‑94 (MQ=60)

             GTGTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATA                                                                                  <  SRR4302537.2050496/94‑1 (MQ=60)

              TTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATAC                                                                                 <  SRR4302537.1005265/94‑1 (MQ=60)

               TTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACA                                                                                >  SRR4302537.5122328/1‑94 (MQ=60)

               TTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACA                                                                                >  SRR4302537.5122329/1‑94 (MQ=60)

                 ATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACC                                                                              >  SRR4302537.4366938/1‑94 (MQ=60)

                 ATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACC                                                                              <  SRR4302537.1151399/94‑1 (MQ=60)

                   TTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTA                                                                            >  SRR4302537.5964563/1‑94 (MQ=60)

                    TTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTAC                                                                           <  SRR4302537.3186479/94‑1 (MQ=60)

                    TTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTAC                                                                           <  SRR4302537.4802827/94‑1 (MQ=60)

                     TGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACG                                                                          <  SRR4302537.4810259/94‑1 (MQ=60)

                     TGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACG                                                                          <  SRR4302537.1021509/94‑1 (MQ=60)

                     TGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACG                                                                          >  SRR4302537.120224/1‑94 (MQ=60)

                       ATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGC                                                                        >  SRR4302537.4850410/1‑94 (MQ=60)

                          AGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTT                                                                     >  SRR4302537.6448864/1‑94 (MQ=60)

                          AGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTT                                                                     >  SRR4302537.5008663/1‑94 (MQ=60)

                          AGCCGGAAATCACGAATACAGNCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTT                                                                     <  SRR4302537.2257395/94‑1 (MQ=60)

                            CCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTA                                                                   <  SRR4302537.1599875/94‑1 (MQ=60)

                            CCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTA                                                                   <  SRR4302537.3325371/94‑1 (MQ=60)

                            CCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTA                                                                   >  SRR4302537.3004550/1‑94 (MQ=60)

                             CGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTAC                                                                  >  SRR4302537.2465448/1‑94 (MQ=60)

                             CGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTAC                                                                  <  SRR4302537.3855572/94‑1 (MQ=60)

                             CGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTAC                                                                  <  SRR4302537.3662218/94‑1 (MQ=60)

                             CGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAAGGGCTTCACCCCAATACACCTACGGCGTTTAC                                                                  >  SRR4302537.2776861/1‑94 (MQ=60)

                              GGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACA                                                                 <  SRR4302537.5614801/94‑1 (MQ=60)

                              GGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACA                                                                 >  SRR4302537.2410368/1‑94 (MQ=60)

                              GGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACA                                                                 >  SRR4302537.347401/1‑94 (MQ=60)

                               GAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAG                                                                >  SRR4302537.2941320/1‑94 (MQ=60)

                                AGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGG                                                               <  SRR4302537.6239075/94‑1 (MQ=60)

                                AGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGG                                                               <  SRR4302537.2391518/94‑1 (MQ=60)

                                  ATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTA                                                             >  SRR4302537.2739479/1‑94 (MQ=60)

                                   TCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTAT                                                            <  SRR4302537.2836188/94‑1 (MQ=60)

                                   ACACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGTGAAAGGGGCTTCACCCCAATACTCCTACGGCGTTTACAGGTAT                                                            <  SRR4302537.844509/94‑1 (MQ=60)

                                     ACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATAC                                                          >  SRR4302537.3751844/1‑94 (MQ=60)

                                      CGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACT                                                         <  SRR4302537.5228634/94‑1 (MQ=60)

                                       GAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTC                                                        >  SRR4302537.1840914/1‑94 (MQ=60)

                                       GAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTC                                                        <  SRR4302537.6099789/94‑1 (MQ=60)

                                       GAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTCTACAGGTATACTC                                                        >  SRR4302537.6126154/1‑94 (MQ=60)

                                        AATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCG                                                       >  SRR4302537.717695/1‑94 (MQ=60)

                                        AATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGAGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCG                                                       >  SRR4302537.4353126/1‑94 (MQ=60)

                                         ATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGC                                                      >  SRR4302537.479671/1‑94 (MQ=60)

                                          TACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCT                                                     <  SRR4302537.4802135/94‑1 (MQ=60)

                                           ACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTA                                                    <  SRR4302537.1982774/94‑1 (MQ=60)

                                            CAGCCGTGCTCCGATGCCTGACGTGCCTTATTAGGCAGGGGCTAAACGGGC‑TCACCCCAATCCACCTGCGGCGTTTACAGGTATACTCGCTAAA                                                  <  SRR4302537.4996425/94‑1 (MQ=60)

                                            CAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAA                                                   <  SRR4302537.6358945/94‑1 (MQ=60)

                                            CAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAA                                                   <  SRR4302537.6358944/94‑1 (MQ=60)

                                            CAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGC‑TCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAA                                                  <  SRR4302537.4996426/94‑1 (MQ=60)

                                             AGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAA                                                  <  SRR4302537.719660/94‑1 (MQ=60)

                                             AGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACATACGGCGTTTACAGGTATACTCGCTAAA                                                  >  SRR4302537.4426934/1‑94 (MQ=60)

                                               CCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAA                                                >  SRR4302537.123647/1‑94 (MQ=60)

                                               CCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAA                                                >  SRR4302537.1249662/1‑94 (MQ=60)

                                               CCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAA                                                >  SRR4302537.3514276/1‑94 (MQ=60)

                                                 GTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATT                                              <  SRR4302537.1385321/94‑1 (MQ=60)

                                                  TGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTA                                             <  SRR4302537.1720250/94‑1 (MQ=60)

                                                    CGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATT                                           <  SRR4302537.2504899/94‑1 (MQ=60)

                                                     GCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTC                                          >  SRR4302537.5477816/1‑94 (MQ=60)

                                                     GCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTC                                          <  SRR4302537.5065000/94‑1 (MQ=60)

                                                       CGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAG                                        <  SRR4302537.5392698/94‑1 (MQ=60)

                                                       CGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAG                                        <  SRR4302537.2253190/94‑1 (MQ=60)

                                                       CGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAG                                        >  SRR4302537.2605974/1‑94 (MQ=60)

                                                         ATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACGCCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCG                                      >  SRR4302537.4330293/1‑94 (MQ=60)

                                                         ATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCG                                      <  SRR4302537.4008517/94‑1 (MQ=60)

                                                          TGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGG                                     <  SRR4302537.657891/94‑1 (MQ=60)

                                                            CCTGACGTGCCTTATTAGGCAGGGGCTAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGT                                   <  SRR4302537.5162301/94‑1 (MQ=60)

                                                            CCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGT                                   >  SRR4302537.2213422/1‑94 (MQ=60)

                                                            CCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGT                                   <  SRR4302537.2906643/94‑1 (MQ=60)

                                                              TGACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTT                                 <  SRR4302537.5666318/94‑1 (MQ=60)

                                                               GACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTG                                >  SRR4302537.1360871/1‑94 (MQ=60)

                                                                ACGTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGG                               <  SRR4302537.5775998/94‑1 (MQ=60)

                                                                  GTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTCTACTCGCTAAAAATTATTCAGCGGGTTTGGAA                             >  SRR4302537.5253781/1‑94 (MQ=60)

                                                                  GTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAA                             >  SRR4302537.1582646/1‑94 (MQ=60)

                                                                  GTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAA                             <  SRR4302537.1148707/94‑1 (MQ=60)

                                                                  GTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAA                             >  SRR4302537.2897095/1‑94 (MQ=60)

                                                                  GTGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAA                             <  SRR4302537.4762082/94‑1 (MQ=60)

                                                                   TGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAA                            >  SRR4302537.5752960/1‑94 (MQ=60)

                                                                   TGCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAA                            >  SRR4302537.1875223/1‑94 (MQ=60)

                                                                    GCCTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAAC                           >  SRR4302537.4739435/1‑94 (MQ=60)

                                                                      CTTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAA                         >  SRR4302537.6463471/1‑94 (MQ=60)

                                                                       TTATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAA                        <  SRR4302537.1635397/94‑1 (MQ=60)

                                                                        TATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAG                       <  SRR4302537.2916020/94‑1 (MQ=60)

                                                                        TATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAG                       <  SRR4302537.3588702/94‑1 (MQ=60)

                                                                         ATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGA                      >  SRR4302537.6411106/1‑94 (MQ=60)

                                                                         ATTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGA                      >  SRR4302537.602875/1‑94 (MQ=60)

                                                                          TTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGAT                     <  SRR4302537.4496669/94‑1 (MQ=60)

                                                                          TTAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGAT                     <  SRR4302537.2960903/94‑1 (MQ=60)

                                                                          TTAGGCAGGGGCGAAAGGAACTTCACCCCAATACACCTACGGCGTTTACAGATATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGAT                     <  SRR4302537.3483470/94‑1 (MQ=60)

                                                                           TAGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATG                    >  SRR4302537.1383882/1‑94 (MQ=60)

                                                                            AGGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGG                   >  SRR4302537.6205595/1‑94 (MQ=60)

                                                                             GGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGC                  >  SRR4302537.560352/1‑94 (MQ=60)

                                                                             GGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGC                  <  SRR4302537.3296749/94‑1 (MQ=60)

                                                                             GGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGC                  >  SRR4302537.3879777/1‑94 (MQ=60)

                                                                             GGCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGC                  >  SRR4302537.2779997/1‑94 (MQ=60)

                                                                              GCAGGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCA                 <  SRR4302537.4512733/94‑1 (MQ=60)

                                                                                 GGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAA              <  SRR4302537.6504082/94‑1 (MQ=60)

                                                                                 GGGGCGAAAGGGGCTTCACCCCAATACACCTACGGCGGTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAA              >  SRR4302537.3846097/1‑94 (MQ=60)

                                                                                    GCGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTT           <  SRR4302537.1798331/94‑1 (MQ=60)

                                                                                     CGAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTA          >  SRR4302537.1464245/1‑94 (MQ=60)

                                                                                      GAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTAC         >  SRR4302537.3082494/1‑94 (MQ=60)

                                                                                      GAAAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTAC         >  SRR4302537.514874/1‑94 (MQ=60)

                                                                                        AAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCT       <  SRR4302537.1667024/94‑1 (MQ=60)

                                                                                        AAGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCT       >  SRR4302537.3946249/1‑94 (MQ=60)

                                                                                         AGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCTT      >  SRR4302537.3378958/1‑94 (MQ=60)

                                                                                         AGGGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCTT      >  SRR4302537.5844692/1‑94 (MQ=60)

                                                                                           GGGCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCTTAC    >  SRR4302537.5618032/1‑94 (MQ=60)

                                                                                             GCTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCTTACAA  <  SRR4302537.3806404/94‑1 (MQ=60)

CTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCTTACAA  >  NC_000913/375830‑376016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`